东北梅花鹿线粒体COⅡ基因序列的遗传结构及其系统发育分析
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- 英文论文题名：Genetic structure and phylogenetic analysis of the mtDNA COⅡ gene in Northeastern sika deer
- 论文作者：徐佳萍 ; 荣敏 ; 杨福合 ; 邢秀梅
- 英文论文作者：Xu JiaPing ; Rong Min ; Yang Fuhe ; Xing Xiumei ; Institute of Special Economic Animal and Plat Science ; Chinese Academy of Agricultural Sciebces ; State Key Laboratory of Special Economic Animal Molecular Biology ; Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction
- 年：2016
- 作者机构：中国农业科学院特产研究所吉林省特种经济动物分子生物学省部共建实验室特种经济动物种质资源遗传改良重点开放实验室;
- 论文关键词：东北梅花鹿 ; mtDNA COⅡ基因 ; 遗传结构 ; 系统发育
- 英文论文关键词：Northeastern sika deer ; mtDNA CO Ⅱ gene ; genetic structure ; phylogeny
- 会议召开时间：2016-06-01
- 会议录名称：“十二五” 鹿科学研究进展
- 语种：中文
- 分类号：S825
- 学会代码：ZXMY
- 学会名称：中国畜牧业协会
- 页数：7
- 文件大小：303k
- 原文格式：O
摘要
采用基因测序的方法,对3种东北梅花鹿的COⅡ基因片段进行了PCR扩增和测序。结果表明,3种东北梅花鹿COⅡ基因片段的长度均为740bp,A+T含量都大于G+C的含量。3个品种单倍型多样性较高(0.673~0.717),就单个品种而言,敖东梅花鹿的遗传多样性最高。
MtDNA CO Ⅱ gene fragments of three Northeastern sika deer species were amplified using PCR method,and the products were purified and sequenced.The result showed that all the mtDNA CO Ⅱ gene fragments had 740 bp,and had greater amount of the A + T pairs than the G + C pairs.As a whole,the haplotype diversity was high(0.673 ~0.717).In a single species,aodong sika deer had a much higher genetic diversities.
MtDNA CO Ⅱ gene fragments of three Northeastern sika deer species were amplified using PCR method,and the products were purified and sequenced.The result showed that all the mtDNA CO Ⅱ gene fragments had 740 bp,and had greater amount of the A + T pairs than the G + C pairs.As a whole,the haplotype diversity was high(0.673 ~0.717).In a single species,aodong sika deer had a much higher genetic diversities.
引文
[1]Whitehead G K.The Encyclopedia of Deer[M].Shrewsbury:Swann-Hill,1993:355.
[2]盛和林.中国鹿类动物[M].上海:华东师范大学出版社,1992:8.
[3]汪松.中国濒危动物红皮书.兽类[M].北京:科学出版社,1998:266-269.
[4]Avise JC,Amold J,Ball RM,et al.Intraspecific phylogeography them itochondrial DNA bridge between population genetics and system atics[J].Annu Rev Ecol Evol Syst,1987,18:489-522.
[5]Harrison RG.Animal mitochondrial DNA as a genetic marker in population and evolutionary biology[J].Trends Ecol Evol,1989,4(1):6-11.
[6]Jermiin L S,Crozier R H.The cytochrome b region in the mitochondrial DNA of the ant Teraponera rufoniger;sequence divergence in Hymenoptera may be associated with nucleotide content[J].J Mol Evol,1994,38(3):282-294.
[7]Whitlock MC,MoCauley DE.Indirect measures of gene flow and migration;Fst not equal to 1/(4Nm+1)[J].Heredity,1999,82(Pt2):117-125.
[8]杨燕.中国七个地方绵羊品种微卫星DNA的遗传多样性研究[D].杨凌:西北农林科技大学.2004.
[2]盛和林.中国鹿类动物[M].上海:华东师范大学出版社,1992:8.
[3]汪松.中国濒危动物红皮书.兽类[M].北京:科学出版社,1998:266-269.
[4]Avise JC,Amold J,Ball RM,et al.Intraspecific phylogeography them itochondrial DNA bridge between population genetics and system atics[J].Annu Rev Ecol Evol Syst,1987,18:489-522.
[5]Harrison RG.Animal mitochondrial DNA as a genetic marker in population and evolutionary biology[J].Trends Ecol Evol,1989,4(1):6-11.
[6]Jermiin L S,Crozier R H.The cytochrome b region in the mitochondrial DNA of the ant Teraponera rufoniger;sequence divergence in Hymenoptera may be associated with nucleotide content[J].J Mol Evol,1994,38(3):282-294.
[7]Whitlock MC,MoCauley DE.Indirect measures of gene flow and migration;Fst not equal to 1/(4Nm+1)[J].Heredity,1999,82(Pt2):117-125.
[8]杨燕.中国七个地方绵羊品种微卫星DNA的遗传多样性研究[D].杨凌:西北农林科技大学.2004.