酪蛋白非磷酸肽对大鼠肌肉合成的作用

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• 英文论文题名：Effects ofcasein non-phosphopeptide on muscles synthesis in rats
• 论文作者：张娜 ; 郭长慧 ; 石彦国
• 英文论文作者：Zhang Na ; Guo Changhui ; Shi Yanguo ; College of Food Engineering ; Harbin University of Commerce
• 年：2016
• 作者机构：哈尔滨商业大学食品工程学院;
• 论文关键词：酪蛋白非磷酸肽 ; 肌肉衰减症 ; CT扫描
• 英文论文关键词：casein non-phosphopeptide ; sarcopenia ; CT scanning
• 会议召开时间：2016-11-09
• 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
• 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
• 语种：中文
• 分类号：TS201.4
• 学会代码：ZGSP
• 会议名称：中国食品科学技术学会第十三届年会
• 会议地点：中国北京
• 主办单位：中国食品科学技术学会
• 学会名称：中国食品科学技术学会
• 页数：2
• 文件大小：109k
• 原文格式：O
• 会议级别：全国

摘要

研究表明50岁患肌肉衰减症人群为2%,80岁以上患肌肉衰减症人群则超过50%。肌肉衰减症会导致身体虚弱,运动平衡能力下降,这些可能会影响新陈代谢,从而引起诸如糖尿病、关节炎、骨质疏松和心脏病等症状的发生。研究发现富含亮氨酸的α-乳清蛋白可以抑制肌肉衰减症引发的肌肉减少。酪蛋白非磷酸肽(casein nonphosphopeptide,CNPP)富含亮氨酸是生产酪蛋白磷酸肽的副产物。本研究的目的是探讨CNPP对健康大鼠肌肉生长的影响。10只32周龄雄性大鼠,被分为饲喂CNPP组和乳清蛋白组,每组分为三个剂量水平(高剂量,10 g/kg/d;中剂量,5 g/kg/d,低剂量,2.5g/kg/d)和空白(0g/kg/d),试验时间为50天。每个饲喂组分为抗阻力运动(resistive exercise group,REG)和不运动(no exercise group,NEG)两组。抗阻力运动爬5min楼梯休息1min,每天3组。通过横向螺旋CT扫描仪检测大鼠在第0天,15天,25天,35天和50天时肌肉横截面积。根据解剖学特征确定肌肉群。采用CT软件确定图像中肌肉群的区域。采用试剂盒测定血液中生长激素、胰岛素和睾酮的水平。利用SPSS统计软件对结果进行分析。结果表明在50天时饲喂中剂量CNPP的抗阻力运动大鼠躯干及下肢根部肌群,背部肌群,和上肢左侧根部肌群的横截面积显著增加(P<0.05)。在50天时饲喂高剂量CNPP的抗阻力运动大鼠躯干及下肢根部肌群和腰椎周围肌群的横截面积也显著增加(P<0.05)。此外,在50天时饲喂中剂量CNPP的不运动大鼠躯干及下肢根部肌群,肩胛内测肌群,和上肢左侧根部肌群的横截面积显著增加(P<0.05)。在50天时饲喂CNPP的REG和NEG血液中生长激素、胰岛素和睾酮的水平较0天显著增加(P<0.05)。我们研究表明富含亮氨酸的CNPP可以刺激大鼠的某些部位肌肉的合成,且增加血液中生长激素、胰岛素和睾酮的水平。
About 2%of the population at the age of 50 suffer from sarcopenia,and the proportion is more than 50%after the age of 80.Sarcopenia may lead to physical weakness and reduced balance and mobility,which in turn have possible metabolic effects such as diabetes,arthritis,osteoporosis,and heart diseases.The leucine-rich whey protein α-lactalbumin has been found to counteract the muscle loss caused by sarcopenia.Casein non-phosphopeptide(CNPP) is a byproduct from casein phosphopeptide production,and is rich in leucine.The objective of this study was to investigate the effect of CNPP on the muscle development of healthy rats.Ten thirty-two weeks old male rats were fed one of the four dosage levels of CNPP or α-lactalbumin(high dose,10 g/kg/d;moderate dose,5 g/kg/d;low dose,2.5 g/kg/d;and blank,0 g/kg/d) for 50 days.Each feeding group was divided into two exercise groups:resistive exercise group(REG) and no exercise group(NEG).The resistive exercise consisted of three sets of 5 min climbing of stairs with 1 min intervals daily.At 0,15,25,35,and 50 days of feeding,rats were anesthetized and their cross-sectional body images were obtained using a transverse spiral CT scanner.Muscle groups were identified based on anatomical features.The areas of these muscle groups in the obtained images were determined using the CT software.The blood levels of growth hormone,insulin,and testosterone were determined using assay kits.Results were analyzed statistically using SPSS software.The results showed that the cross-sectional area of the trunk,back muscle group,and left upper limb muscle roots of the REG rats fed the moderate dose CNPP for 50 days increased significantly(P < 0.05).The cross-sectional area of the trunk and lumbar muscle group of the REG rats fed the high dose CNPP for 50 days also increased significantly(P < 0.05).Furthermore,the cross-sectional area of the trunk,beta scapular muscle,and left upper limb muscle roots of the NEG rats fed the moderate dose CNPP for 50 days increased significantly(P < 0.05).In both CNPP-fed REG and NEG groups,the blood levels of growth hormone,insulin,and testosterone increased significantly after feeding for 50 days compared to those on day 0(P<0.05).Our studies have demonstrated that the leucine-rich CNPP stimulates the synthesis of certain rat muscles,and increases the blood levels of insulin,growth hormone,and testosterone.

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