miR-21 Regulates TNF-α-Induced CD40 Expression via SIRT1-NF-κB Pathway in Renal Inner Medulla Collecting Duct Cells
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- 英文论文题名：miR-21 Regulates TNF-α-Induced CD40 Expression via SIRT1-NF-κB Pathway in Renal Inner Medulla Collecting Duct Cells
- 论文作者：林琴琴 ; 耿元文 ; 田振军
- 年：2016
- 作者机构：燕山大学体育学院;陕西师范大学体育学院;
- 英文论文关键词：miR-21 ; SIRT1 ; CD40 ; NF-κB ; IMCD cells
- 会议召开时间：2016-10-21
- 会议录名称：第四届（2016）全国运动生理与生物化学学术会议——运动·体质·健康论文摘要汇编
- 语种：英文
- 分类号：R692
- 学会代码：ZGTK
- 会议名称：第四届（2016）全国运动生理与生物化学学术会议——运动·体质·健康
- 会议地点：中国江苏无锡
- 主办单位：中国体育科学学会运动生理与生物化学分会
- 学会名称：中国体育科学学会
- 页数：2
- 文件大小：79k
- 原文格式：O
- 会议级别：全国
摘要
Objective:To study the potential effect of miR-21 and SIRT1 on CD40 expression induced by TNF-α and underlying mechanisms in renal inner medulla collecting duct(IMCD)cells.Method:After stimulation with the indicated times and doses of TNF-α,miR-21 mRNA levels were analyzed by RT-qPCR.Following transfection with miR-21 mimics or miR-21 inhibitor,the mRNA and protein expression of SIRT1 and CD40 were identified by RT-qPCR and Western blot,respectively.Furthermore,the underlying molecular mechanism was also investigated.Results:TNF-α increased miR-21,CD40 and Ac-p65 and reduced SIRT1 expression.miR-21 mimics increased SIRT1 and reduced Ac-p65 and CD40 expressions,the corresponding changes were observed by miR-21 inhibitor,which was impeded by pre-treating with SIRT1 siRNA.Overexpression of SIRT1 reduced CD40 and Ac-p65 expression,which was reversed by silencing SIRT1 and augmented by NF-κB siRNA.Conclusion:miR-21 inhibits TNF-α-induced CD40 expression in IMCD cells via the SIRT1-NF-κB signaling pathway.
Objective:To study the potential effect of miR-21 and SIRT1 on CD40 expression induced by TNF-α and underlying mechanisms in renal inner medulla collecting duct(IMCD)cells.Method:After stimulation with the indicated times and doses of TNF-α,miR-21 mRNA levels were analyzed by RT-qPCR.Following transfection with miR-21 mimics or miR-21 inhibitor,the mRNA and protein expression of SIRT1 and CD40 were identified by RT-qPCR and Western blot,respectively.Furthermore,the underlying molecular mechanism was also investigated.Results:TNF-α increased miR-21,CD40 and Ac-p65 and reduced SIRT1 expression.miR-21 mimics increased SIRT1 and reduced Ac-p65 and CD40 expressions,the corresponding changes were observed by miR-21 inhibitor,which was impeded by pre-treating with SIRT1 siRNA.Overexpression of SIRT1 reduced CD40 and Ac-p65 expression,which was reversed by silencing SIRT1 and augmented by NF-κB siRNA.Conclusion:miR-21 inhibits TNF-α-induced CD40 expression in IMCD cells via the SIRT1-NF-κB signaling pathway.
Objective:To study the potential effect of miR-21 and SIRT1 on CD40 expression induced by TNF-α and underlying mechanisms in renal inner medulla collecting duct(IMCD)cells.Method:After stimulation with the indicated times and doses of TNF-α,miR-21 mRNA levels were analyzed by RT-qPCR.Following transfection with miR-21 mimics or miR-21 inhibitor,the mRNA and protein expression of SIRT1 and CD40 were identified by RT-qPCR and Western blot,respectively.Furthermore,the underlying molecular mechanism was also investigated.Results:TNF-α increased miR-21,CD40 and Ac-p65 and reduced SIRT1 expression.miR-21 mimics increased SIRT1 and reduced Ac-p65 and CD40 expressions,the corresponding changes were observed by miR-21 inhibitor,which was impeded by pre-treating with SIRT1 siRNA.Overexpression of SIRT1 reduced CD40 and Ac-p65 expression,which was reversed by silencing SIRT1 and augmented by NF-κB siRNA.Conclusion:miR-21 inhibits TNF-α-induced CD40 expression in IMCD cells via the SIRT1-NF-κB signaling pathway.
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