Regulation of ApoE expression by lipopolysaccharide in head and neck squamous carcinoma cells:Roles for nuclear factor-κB and mitogen-activated protein kinase
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- 英文论文题名：Regulation of ApoE expression by lipopolysaccharide in head and neck squamous carcinoma cells:Roles for nuclear factor-κB and mitogen-activated protein kinase
- 论文作者：Qian Xuesong
- 英文论文作者：Qian Xuesong ; hebei medical university
- 年：2016
- 作者机构：hebei medical university;
- 英文论文关键词：Head and neck squamous cell carcinoma(HNSCC) ; apolipoprotein(apoE) ; lipopolysaccharide(LPS) ; mito
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R739.91
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1353k
- 原文格式：D
- 会议级别：全国
摘要
Purpose: ApoE is expressed in multiple mammalian cell types in which it supports cell differentiation function. The atheroprotective role of apoE has been well established for many years and has been recently identified as a potential tumorassociated marker in some kinds of malignancies. However, its expression and modulation by inflammatory factors such as lipopolysaccharide in head and neck squamous carcinoma(HNSC) cells has yet to be studied.Method: In this report we demonstrated the apoE expression and its regulation by LPS in HNSC cells. We also investigated the signaling pathways involved in induction of apoE gene expression in response to lipopolysaccharide(LPS) treatment. Result: It was interesting to see that the inhibition of either p38 mitogen-activated protein kinases(MAPK) or the Jun Nterminal kinase(JNK) enhanced LPS-induced apoE expression. Moreover, small interference RNA(siRNA) of NF-κB p65 attenuated cellular amount of p65 and suppressed LPS-induced apoE expression. Inhibition of apoE expression usingan apoE-specific siRNAin Fadu cells led to apoptosis and reduced tumor cell invasiveness. Conclusion: Our finding indicated apoE expression was modulated by NF-κB and MAPK in HNSC cells, apoE expression is important for tumor cell apoptosis and invasion.
Purpose: ApoE is expressed in multiple mammalian cell types in which it supports cell differentiation function. The atheroprotective role of apoE has been well established for many years and has been recently identified as a potential tumorassociated marker in some kinds of malignancies. However, its expression and modulation by inflammatory factors such as lipopolysaccharide in head and neck squamous carcinoma(HNSC) cells has yet to be studied.Method: In this report we demonstrated the apoE expression and its regulation by LPS in HNSC cells. We also investigated the signaling pathways involved in induction of apoE gene expression in response to lipopolysaccharide(LPS) treatment. Result: It was interesting to see that the inhibition of either p38 mitogen-activated protein kinases(MAPK) or the Jun Nterminal kinase(JNK) enhanced LPS-induced apoE expression. Moreover, small interference RNA(siRNA) of NF-κB p65 attenuated cellular amount of p65 and suppressed LPS-induced apoE expression. Inhibition of apoE expression usingan apoE-specific siRNAin Fadu cells led to apoptosis and reduced tumor cell invasiveness. Conclusion: Our finding indicated apoE expression was modulated by NF-κB and MAPK in HNSC cells, apoE expression is important for tumor cell apoptosis and invasion.
Purpose: ApoE is expressed in multiple mammalian cell types in which it supports cell differentiation function. The atheroprotective role of apoE has been well established for many years and has been recently identified as a potential tumorassociated marker in some kinds of malignancies. However, its expression and modulation by inflammatory factors such as lipopolysaccharide in head and neck squamous carcinoma(HNSC) cells has yet to be studied.Method: In this report we demonstrated the apoE expression and its regulation by LPS in HNSC cells. We also investigated the signaling pathways involved in induction of apoE gene expression in response to lipopolysaccharide(LPS) treatment. Result: It was interesting to see that the inhibition of either p38 mitogen-activated protein kinases(MAPK) or the Jun Nterminal kinase(JNK) enhanced LPS-induced apoE expression. Moreover, small interference RNA(siRNA) of NF-κB p65 attenuated cellular amount of p65 and suppressed LPS-induced apoE expression. Inhibition of apoE expression usingan apoE-specific siRNAin Fadu cells led to apoptosis and reduced tumor cell invasiveness. Conclusion: Our finding indicated apoE expression was modulated by NF-κB and MAPK in HNSC cells, apoE expression is important for tumor cell apoptosis and invasion.
引文