Enhanced triterpene acid production by Ganoderma lucidum using a feeding stimulus with integration of a two-stage pH control strategy
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- 英文论文题名：Enhanced triterpene acid production by Ganoderma lucidum using a feeding stimulus with integration of a two-stage pH control strategy
- 论文作者：刘高强 ; 王晓玲 ; 杨海龙
- 英文论文作者：College of Life Science and Technology ; Central South University of Forestry and Technology ; College of Life and Environmental Science ; Wenzhou University
- 年：2016
- 作者机构：College of Life Science and Technology,Central South University of Forestry and Technology;College of Life and Environmental Science,Wenzhou University;
- 英文论文关键词：Ganoderma lucidum ; Triterpene acids ; Fermentation ; Process control ; Biochemical engineering ; Bioprocesses
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：英文
- 分类号：TQ921
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：1
- 文件大小：721k
- 原文格式：D
- 会议级别：全国
摘要
BACKGROUND:Triterpenes(especially triterpene acids) is one of the major physiologically active components of Ganoderma lucidum.However,submerged fermentation techniques of triterpenes is not optimized for commercial use,as total triterpene yield is still very low.Our aims are to develop a two-stage p H control strategy integrated with a stimulus,an ether extract(EECM,from the medicinal insect,Catharsius molossus),for enhanced G.lucidum triterpene acid production.RESULTS:The triterpene acid production of 302.76 mg/L was obtained in the G.lucidum culture with EECM,which was higher by 60% compared with control cultures not fed EECM.Based on the feeding EECM method,a new two-stage p H control strategy was integrated resulting in triterpene acid yields of 361.47 mg/L.Subsequent studies optimized interactions between EECM feeding and two-stage p H control strategies,which resulted in an optimal EECM concentration of 185.89 mg/L,optimal p H values of 5.07 in the first 72 h incubation and 4.37 after 72 h with a maximum triterpene acid concentration of 449.37 mg/L.This represented a 137% increase compared to that of the control cultures.Furthermore,using a 150-L fermenter under these conditions,a triterpene acid concentration of 431.83 mg/L was achieved.CONCLUSIONS:Enhanced production of triterpene acid in submerged cultures of G.lucidum was achieved using an EECM feeding stimulus integrated with a two-stage p H control strategy in a large scale.
BACKGROUND:Triterpenes(especially triterpene acids) is one of the major physiologically active components of Ganoderma lucidum.However,submerged fermentation techniques of triterpenes is not optimized for commercial use,as total triterpene yield is still very low.Our aims are to develop a two-stage p H control strategy integrated with a stimulus,an ether extract(EECM,from the medicinal insect,Catharsius molossus),for enhanced G.lucidum triterpene acid production.RESULTS:The triterpene acid production of 302.76 mg/L was obtained in the G.lucidum culture with EECM,which was higher by 60% compared with control cultures not fed EECM.Based on the feeding EECM method,a new two-stage p H control strategy was integrated resulting in triterpene acid yields of 361.47 mg/L.Subsequent studies optimized interactions between EECM feeding and two-stage p H control strategies,which resulted in an optimal EECM concentration of 185.89 mg/L,optimal p H values of 5.07 in the first 72 h incubation and 4.37 after 72 h with a maximum triterpene acid concentration of 449.37 mg/L.This represented a 137% increase compared to that of the control cultures.Furthermore,using a 150-L fermenter under these conditions,a triterpene acid concentration of 431.83 mg/L was achieved.CONCLUSIONS:Enhanced production of triterpene acid in submerged cultures of G.lucidum was achieved using an EECM feeding stimulus integrated with a two-stage p H control strategy in a large scale.
BACKGROUND:Triterpenes(especially triterpene acids) is one of the major physiologically active components of Ganoderma lucidum.However,submerged fermentation techniques of triterpenes is not optimized for commercial use,as total triterpene yield is still very low.Our aims are to develop a two-stage p H control strategy integrated with a stimulus,an ether extract(EECM,from the medicinal insect,Catharsius molossus),for enhanced G.lucidum triterpene acid production.RESULTS:The triterpene acid production of 302.76 mg/L was obtained in the G.lucidum culture with EECM,which was higher by 60% compared with control cultures not fed EECM.Based on the feeding EECM method,a new two-stage p H control strategy was integrated resulting in triterpene acid yields of 361.47 mg/L.Subsequent studies optimized interactions between EECM feeding and two-stage p H control strategies,which resulted in an optimal EECM concentration of 185.89 mg/L,optimal p H values of 5.07 in the first 72 h incubation and 4.37 after 72 h with a maximum triterpene acid concentration of 449.37 mg/L.This represented a 137% increase compared to that of the control cultures.Furthermore,using a 150-L fermenter under these conditions,a triterpene acid concentration of 431.83 mg/L was achieved.CONCLUSIONS:Enhanced production of triterpene acid in submerged cultures of G.lucidum was achieved using an EECM feeding stimulus integrated with a two-stage p H control strategy in a large scale.
引文
Miyamoto I,Liu J,Shimizu K,Sato M,Kukita A,Kukita T,Kondo R,2009.Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum.EUR J Pharmacol 602:1-7.
Chen NH,Liu JW and Zhong JJ,2010.Ganoderic acid T inhibits tumor invasion in vitro and in vivo through inhibition of MMP expression.Pharmacol Rep 62:150-163.
Liu GQ,Zhang KC,2007.Enhancement of polysaccharides production in Ganoderma lucidum by the addition of ethyl acetate extracts from Eupolyphaga sinensis and Catharsius molossus.Appl Microbiol Biotechnol74:572-577.
Liu GQ,Xiao HX,Wang XL,Zhao Y,Zhang YG,Ren GP,2011.Stimulated production of triterpenoids of Ganoderma lucidum by an ether extract from the medicinal insect,Catharsius molossus and identification of the key stimulating active components.Appl Biochem Biotechnol 165:87-97.
Chen NH,Liu JW and Zhong JJ,2010.Ganoderic acid T inhibits tumor invasion in vitro and in vivo through inhibition of MMP expression.Pharmacol Rep 62:150-163.
Liu GQ,Zhang KC,2007.Enhancement of polysaccharides production in Ganoderma lucidum by the addition of ethyl acetate extracts from Eupolyphaga sinensis and Catharsius molossus.Appl Microbiol Biotechnol74:572-577.
Liu GQ,Xiao HX,Wang XL,Zhao Y,Zhang YG,Ren GP,2011.Stimulated production of triterpenoids of Ganoderma lucidum by an ether extract from the medicinal insect,Catharsius molossus and identification of the key stimulating active components.Appl Biochem Biotechnol 165:87-97.