Co-transplantation of Hematopoietic Stem Cells and Cxcr4 Gene Transduced Mesenchymal Stem Cells Promotes Hematopoiesis
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- 英文论文题名：Co-transplantation of Hematopoietic Stem Cells and Cxcr4 Gene Transduced Mesenchymal Stem Cells Promotes Hematopoiesis
- 论文作者：Chen Wei ; Li Miao ; Zang Yu ; Yan Zhiling ; Cheng Hai ; Pan Bin ; Cao Jiang ; Wu Qingyun ; Zhao Kai ; Zhu Feng ; Zeng Lingyu ; Li Zhenyu ; Xu Kailin
- 英文论文作者：Chen Wei ; Li Miao ; Zang Yu ; Yan Zhiling ; Cheng Hai ; Pan Bin ; Cao Jiang ; Wu Qingyun ; Zhao Kai ; Zhu Feng ; Zeng Lingyu ; Li Zhenyu ; Xu Kailin ; Department of Hematology ; the Affiliated Hospital of Xuzhou medical College ; Xuzhou Children's Hospital
- 年：2016
- 作者机构：Department of Hematology,the Affiliated Hospital of Xuzhou medical College;Xuzhou Children's Hospital;
- 英文论文关键词：Mesenchymal Stem Cell ; Bone Marrow Transplantation ; CXCR4
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R457.7
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：985k
- 原文格式：D
- 会议级别：全国
摘要
Aims: Mesenchymal stem cells(MSCs) are a promising candidate for cellular therapies. Co-transplantation of MSCs and hematopoietic stem cells(HSCs) promotes successful engraftment and improves hematopoietic recovery. Methods: In this study, the effects of co-transplantation of HSCs and mouse bone marrow(BM)-derived MSCs overexpressing CXCR4(CXCR4-MSC) on CXCR4-MSC homing capacity and the reconstitution potential in lethally irradiated mice were evaluated. Results: Recovery of donor derived peripheral blood leukocytes(PBL) and platelets was accelerated when CXCR4-MSCs were co-transplanted with BM cells. The frequency of c-kit+Sca+Lin-(KSL) HSCs was higher in recipient BM following co-transplantation of CXCR4-MSCs compared with the EGFP-MSC control and the BMT only groups. Surprisingly, the rate of early engraftment of donor derived BM cells in recipients co-transplanted with CXCR4-MSCs was slightly lower than in the absence of MSCs on day 7. Moreover, co-transplantation of CXCR4-MSCs regulated the balance of T helper cells subsets. Hematopoietic tissue reconstitution was evaluated by histopathological analysis of BM and spleen. Co-transplantation of CXCR4-MSCs was shown to promote the recovery of hematopoietic organs. Conclusion: These findings indicate that co-transplantation of CXCR4-MSCs promotes the early phase of hematopoietic recovery and sustained hematopoiesis.
Aims: Mesenchymal stem cells(MSCs) are a promising candidate for cellular therapies. Co-transplantation of MSCs and hematopoietic stem cells(HSCs) promotes successful engraftment and improves hematopoietic recovery. Methods: In this study, the effects of co-transplantation of HSCs and mouse bone marrow(BM)-derived MSCs overexpressing CXCR4(CXCR4-MSC) on CXCR4-MSC homing capacity and the reconstitution potential in lethally irradiated mice were evaluated. Results: Recovery of donor derived peripheral blood leukocytes(PBL) and platelets was accelerated when CXCR4-MSCs were co-transplanted with BM cells. The frequency of c-kit+Sca+Lin-(KSL) HSCs was higher in recipient BM following co-transplantation of CXCR4-MSCs compared with the EGFP-MSC control and the BMT only groups. Surprisingly, the rate of early engraftment of donor derived BM cells in recipients co-transplanted with CXCR4-MSCs was slightly lower than in the absence of MSCs on day 7. Moreover, co-transplantation of CXCR4-MSCs regulated the balance of T helper cells subsets. Hematopoietic tissue reconstitution was evaluated by histopathological analysis of BM and spleen. Co-transplantation of CXCR4-MSCs was shown to promote the recovery of hematopoietic organs. Conclusion: These findings indicate that co-transplantation of CXCR4-MSCs promotes the early phase of hematopoietic recovery and sustained hematopoiesis.
Aims: Mesenchymal stem cells(MSCs) are a promising candidate for cellular therapies. Co-transplantation of MSCs and hematopoietic stem cells(HSCs) promotes successful engraftment and improves hematopoietic recovery. Methods: In this study, the effects of co-transplantation of HSCs and mouse bone marrow(BM)-derived MSCs overexpressing CXCR4(CXCR4-MSC) on CXCR4-MSC homing capacity and the reconstitution potential in lethally irradiated mice were evaluated. Results: Recovery of donor derived peripheral blood leukocytes(PBL) and platelets was accelerated when CXCR4-MSCs were co-transplanted with BM cells. The frequency of c-kit+Sca+Lin-(KSL) HSCs was higher in recipient BM following co-transplantation of CXCR4-MSCs compared with the EGFP-MSC control and the BMT only groups. Surprisingly, the rate of early engraftment of donor derived BM cells in recipients co-transplanted with CXCR4-MSCs was slightly lower than in the absence of MSCs on day 7. Moreover, co-transplantation of CXCR4-MSCs regulated the balance of T helper cells subsets. Hematopoietic tissue reconstitution was evaluated by histopathological analysis of BM and spleen. Co-transplantation of CXCR4-MSCs was shown to promote the recovery of hematopoietic organs. Conclusion: These findings indicate that co-transplantation of CXCR4-MSCs promotes the early phase of hematopoietic recovery and sustained hematopoiesis.
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