Intraspecific variation of residual heterozygosity and its utility for quantitative genetics studies in maize

详细信息    查看官网全文

• 英文论文题名：Intraspecific variation of residual heterozygosity and its utility for quantitative genetics studies in maize
• 论文作者：Nannan Liu ; Yingjie Xiao ; Jianxiao Liu ; Qingchun Pan ; Hong Wang ; Wei Zhan ; Jie Liu ; Xiaohong Yang ; Jianbing Yan
• 英文论文作者：Nannan Liu ; Yingjie Xiao ; Jianxiao Liu ; Qingchun Pan ; Hong Wang ; Wei Zhan ; Jie Liu ; Xiaohong Yang ; Jianbing Yan ; National Key Laboratory of Crop Genetic Improvement ; Huazhong Agricultural University ; College of Informatics ; Huazhong Agricultural University ; National Maize Improvement Center of ChinaBeijing Key Laboratory of Crop Genetic Improvement ; China Agricultural University
• 年：2016
• 作者机构：National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University;College of Informatics,Huazhong Agricultural University;National Maize Improvement Center of ChinaBeijing Key Laboratory of Crop Genetic Improvement,China Agricultural University;
• 英文论文关键词：maize ; residual heterozygosity ; heterogeneous inbred family ; QTL mapping ; recombinant inbred lines
• 会议召开时间：2016-10-09
• 会议录名称：2016年全国植物生物学大会摘要集
• 语种：英文
• 分类号：S513
• 学会代码：ZGZO
• 会议名称：2016年全国植物生物学大会
• 会议地点：中国湖北武汉
• 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
• 学会名称：中国植物学会
• 页数：1
• 文件大小：852k
• 原文格式：D
• 会议级别：全国

摘要

Residual heterozygosity(RH) in advanced inbred progenies of plants benefits quantitative trait locus(QTL) mapping studies. However,knowledge of factors affecting its genome-wide distribution remains limited. In this study,a collection of maize heterogeneous inbred family(HIF) lines was assembled,comprising a set of 12 recombinant inbred line(RIL) populations with 2,196 lines that were genotyped using a Maize50 K commercial SNP chip. Each population revealed 505 to 2095 RH intervals which collapsed to 18,615 unique RH intervals across 12 populations,covering 94.8% of the maize genome. On average,a given RH region was present in 5 different individuals. We found that some genomic regions were enriched in RH segments and seven RH hotspots were identified in the genome. The RH patterns varied significantly across populations,presumably reflecting differences in genetic backgrounds and 8 QTLs were identified for the RH hotspots in and of themselves. QTL fine mapping of kernel tocopherol content demonstrated a potential of the HIF library to efficiently map QTL locations,down to approximately ≤ 1Mb-resolution based on publicly available information for the 12 populations. The library of HIF lines gave us new insights into the RH landscape and its intraspecific variation and provides a useful resource for QTL cloning of agronomic important traits in maize.
Residual heterozygosity(RH) in advanced inbred progenies of plants benefits quantitative trait locus(QTL) mapping studies. However,knowledge of factors affecting its genome-wide distribution remains limited. In this study,a collection of maize heterogeneous inbred family(HIF) lines was assembled,comprising a set of 12 recombinant inbred line(RIL) populations with 2,196 lines that were genotyped using a Maize50 K commercial SNP chip. Each population revealed 505 to 2095 RH intervals which collapsed to 18,615 unique RH intervals across 12 populations,covering 94.8% of the maize genome. On average,a given RH region was present in 5 different individuals. We found that some genomic regions were enriched in RH segments and seven RH hotspots were identified in the genome. The RH patterns varied significantly across populations,presumably reflecting differences in genetic backgrounds and 8 QTLs were identified for the RH hotspots in and of themselves. QTL fine mapping of kernel tocopherol content demonstrated a potential of the HIF library to efficiently map QTL locations,down to approximately ≤ 1Mb-resolution based on publicly available information for the 12 populations. The library of HIF lines gave us new insights into the RH landscape and its intraspecific variation and provides a useful resource for QTL cloning of agronomic important traits in maize.

引文