摘要
A new aerobic,Gram stain-negative,oxidase- and catalase- positive,ovoid or rod-shaped bacterial strain,designated HTCJW17~T,was isolated from the sediment collected from the South China Sea and subjected to a polyphasic taxonomic characterization.The isolate forms small,creamy-white,opaque and circular colonies on agar plates.Growth occurs between 15 and45℃,1-7%(w/v) NaCl and pH 6-9.Phylogenetic analysis based on 16 S rRNA gene sequences showed that strain HTCJW17~T belongs to the family Kordiimonadaceae of the order Kordiimonadales,with highest sequence similarity to Kordiimonas gwangyangensis GW14-5~T(91.1%).The major fatty acids were identified to be summed feature 8(C_(18:1)ω7c/ω6c;56.6%),C_(14:0)(8.5%),C_(14:0)2-OH(8.4%),summed feature 3(C_(16:1)ω7c/C_(16 1)ω6c;6.2%),and C_(16:0)(5.2%).The polar lipids were determined to be phosphatidylcholine,phosphatidylethanolamine,psphatidylglycerol,six unidentified glycolipids and two undentified phospholipids.The respiratory quinone was identified as ubiquinone Q-10.The genomic DNA G+C content was determined to be 56.3 mol%.On the basis of genotypic,chemotaxonomic and phenotypic distinctness,we propose a novel genus,Emcibacter gen.nov.,with Emcibacter nanhaiensis sp.nov.as the type species.The type strain is HTCJW17~T(= CGMCC 1.12471~T= LMG 27419~T=MCCC 1A06723~T).
A new aerobic,Gram stain-negative,oxidase- and catalase- positive,ovoid or rod-shaped bacterial strain,designated HTCJW17~T,was isolated from the sediment collected from the South China Sea and subjected to a polyphasic taxonomic characterization.The isolate forms small,creamy-white,opaque and circular colonies on agar plates.Growth occurs between 15 and45℃,1-7%(w/v) NaCl and pH 6-9.Phylogenetic analysis based on 16 S rRNA gene sequences showed that strain HTCJW17~T belongs to the family Kordiimonadaceae of the order Kordiimonadales,with highest sequence similarity to Kordiimonas gwangyangensis GW14-5~T(91.1%).The major fatty acids were identified to be summed feature 8(C_(18:1)ω7c/ω6c;56.6%),C_(14:0)(8.5%),C_(14:0)2-OH(8.4%),summed feature 3(C_(16:1)ω7c/C_(16 1)ω6c;6.2%),and C_(16:0)(5.2%).The polar lipids were determined to be phosphatidylcholine,phosphatidylethanolamine,psphatidylglycerol,six unidentified glycolipids and two undentified phospholipids.The respiratory quinone was identified as ubiquinone Q-10.The genomic DNA G+C content was determined to be 56.3 mol%.On the basis of genotypic,chemotaxonomic and phenotypic distinctness,we propose a novel genus,Emcibacter gen.nov.,with Emcibacter nanhaiensis sp.nov.as the type species.The type strain is HTCJW17~T(= CGMCC 1.12471~T= LMG 27419~T=MCCC 1A06723~T).
引文