Dietary administration of Bacillus subtilis HAINUP40 enhances growth,digestive enzyme activities,innate immune responses and disease resistance of tilapia,Oreochromis niloticus
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- 英文论文题名：Dietary administration of Bacillus subtilis HAINUP40 enhances growth,digestive enzyme activities,innate immune responses and disease resistance of tilapia,Oreochromis niloticus
- 论文作者：Shifeng Wang ; Haitian Liu ; Yan Cai ; Wei Yuan ; Yongcan Zhou
- 英文论文作者：Shifeng Wang ; Haitian Liu ; Yan Cai ; Wei Yuan ; Yongcan Zhou ; Key Laboratory of Tropical Biological Resources of Ministry of Education ; College of Marine Sciences ; Hainan University
- 年：2016
- 作者机构：Key Laboratory of Tropical Biological Resources of Ministry of Education,College of Marine Sciences,Hainan University;
- 英文论文关键词：Bacillus subtilis HAINUP40 ; Tilapia(Oreochromis niloticus) ; Growth ; Digestive enzyme activity ; Innate immunity ; Protection
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：英文
- 分类号：S965.125
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：53k
- 原文格式：O
- 会议级别：全国
摘要
The probiotic properties of Bacillus subtilis HAINUP40 isolated from the aquatic environment,and the effects of dietary administration of B.subtilis HAINUP40 on the growth performance,intestinal probiotic recovery,digestive enzyme activities,innate immunity and disease resistance of tilapia(Oreochromis niloticus) were evaluated.The probiotic properties investigated include tolerance to simulated gastrointestinal stress,auto-aggregation,cell surface hydrophobicity and extracellular enzyme production.The cell number of B.subtilis changed little after 4 h in simulated gastric fluid at pH = 2.0,3.0,4.0 and small intestinal fluid at pH = 6.8.B.subtilis HAINUP40 revealed a strong auto-aggregation property(34.6-87.0%) after a 24 h incubation period.It exhibited significant cell surface hydrophobicity in xylene(28.8%) and chloroform(41.3%) and produced extracellular proteases and amylase.After tilapia(mean weight = 95.02 ± 7.65 g) were fed with a diet containing 10~8cfu/g B.subtilis HAINUP40,their final body weight,percent weight gain(PWG),specific growth rate(SGR),total antioxidant capacity(T-AOC) and serum superoxide dismutase(SOD) increased significantly(p<0.05) after 8weeks;feed conversion rate(FCR) is significantly lower(p < 0.05) after 8 weeks;the protease and amylase activity in the digestive tract increased significantly(p<0.05) after 4 and 8 weeks;and respiratory bursts and serum lysozyme activity increased significantly(p< 0.05) after 2 weeks.Moreover,being challenged with pathogenic Streptococcus agalactiae for 2 weeks,the relative percent survival(RPS%) is 52.94%.The results of this study strongly suggest that dietary supplement of B.subtilis HAINUP40 can effectively enhances the growth performance,immune response,and disease resistance of Nile tilapia.
The probiotic properties of Bacillus subtilis HAINUP40 isolated from the aquatic environment,and the effects of dietary administration of B.subtilis HAINUP40 on the growth performance,intestinal probiotic recovery,digestive enzyme activities,innate immunity and disease resistance of tilapia(Oreochromis niloticus) were evaluated.The probiotic properties investigated include tolerance to simulated gastrointestinal stress,auto-aggregation,cell surface hydrophobicity and extracellular enzyme production.The cell number of B.subtilis changed little after 4 h in simulated gastric fluid at pH = 2.0,3.0,4.0 and small intestinal fluid at pH = 6.8.B.subtilis HAINUP40 revealed a strong auto-aggregation property(34.6-87.0%) after a 24 h incubation period.It exhibited significant cell surface hydrophobicity in xylene(28.8%) and chloroform(41.3%) and produced extracellular proteases and amylase.After tilapia(mean weight = 95.02 ± 7.65 g) were fed with a diet containing 10~8cfu/g B.subtilis HAINUP40,their final body weight,percent weight gain(PWG),specific growth rate(SGR),total antioxidant capacity(T-AOC) and serum superoxide dismutase(SOD) increased significantly(p<0.05) after 8weeks;feed conversion rate(FCR) is significantly lower(p < 0.05) after 8 weeks;the protease and amylase activity in the digestive tract increased significantly(p<0.05) after 4 and 8 weeks;and respiratory bursts and serum lysozyme activity increased significantly(p< 0.05) after 2 weeks.Moreover,being challenged with pathogenic Streptococcus agalactiae for 2 weeks,the relative percent survival(RPS%) is 52.94%.The results of this study strongly suggest that dietary supplement of B.subtilis HAINUP40 can effectively enhances the growth performance,immune response,and disease resistance of Nile tilapia.
The probiotic properties of Bacillus subtilis HAINUP40 isolated from the aquatic environment,and the effects of dietary administration of B.subtilis HAINUP40 on the growth performance,intestinal probiotic recovery,digestive enzyme activities,innate immunity and disease resistance of tilapia(Oreochromis niloticus) were evaluated.The probiotic properties investigated include tolerance to simulated gastrointestinal stress,auto-aggregation,cell surface hydrophobicity and extracellular enzyme production.The cell number of B.subtilis changed little after 4 h in simulated gastric fluid at pH = 2.0,3.0,4.0 and small intestinal fluid at pH = 6.8.B.subtilis HAINUP40 revealed a strong auto-aggregation property(34.6-87.0%) after a 24 h incubation period.It exhibited significant cell surface hydrophobicity in xylene(28.8%) and chloroform(41.3%) and produced extracellular proteases and amylase.After tilapia(mean weight = 95.02 ± 7.65 g) were fed with a diet containing 10~8cfu/g B.subtilis HAINUP40,their final body weight,percent weight gain(PWG),specific growth rate(SGR),total antioxidant capacity(T-AOC) and serum superoxide dismutase(SOD) increased significantly(p<0.05) after 8weeks;feed conversion rate(FCR) is significantly lower(p < 0.05) after 8 weeks;the protease and amylase activity in the digestive tract increased significantly(p<0.05) after 4 and 8 weeks;and respiratory bursts and serum lysozyme activity increased significantly(p< 0.05) after 2 weeks.Moreover,being challenged with pathogenic Streptococcus agalactiae for 2 weeks,the relative percent survival(RPS%) is 52.94%.The results of this study strongly suggest that dietary supplement of B.subtilis HAINUP40 can effectively enhances the growth performance,immune response,and disease resistance of Nile tilapia.
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