Characterization of the cellulolytic bacteria communities along the gastrointestinal tract of Chinese Mongolian sheep by using PCR-DGGE and real-time PCR analysis
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- 英文论文题名：Characterization of the cellulolytic bacteria communities along the gastrointestinal tract of Chinese Mongolian sheep by using PCR-DGGE and real-time PCR analysis
- 论文作者：Yan Zeng ; Dong Zeng ; Yan Zhang ; Xueqin Ni ; Yurui Tang ; Hui Zhu ; Hesong Wang ; Zhongqiong Yin ; Kangcheng Pan ; Bo Jing
- 英文论文作者：Yan Zeng ; Dong Zeng ; Yan Zhang ; Xueqin Ni ; Yurui Tang ; Hui Zhu ; Hesong Wang ; Zhongqiong Yin ; Kangcheng Pan ; Bo Jing ; Animal Microecology Institute ; College of Veterinary Medicine ; Sichuan Agricultural University ; Key Laboratory of Animal Disease and Human Health of Sichuan Province
- 年：2016
- 作者机构：Animal Microecology Institute,College of Veterinary Medicine,Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province;
- 英文论文关键词：Chinese Mongolian sheep ; gastrointestinal tract ; cellulolytic bacteria ; PCR-DGGE ; real-time PCR
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：英文
- 分类号：S826
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：66k
- 原文格式：O
- 会议级别：全国
摘要
A balanced gastrointestinal microbial ecosystem is crucial for the health and growth of animals.In the gastrointestinal tract(GIT) of ruminants,cellulolytic bacteria aid in the digestion and absorption of nutrients.Rumen contents and feces in ruminants are often used to assess gastrointestinal microbial communities;however,these sites do not guarantee to represent the diversity of microbes found in the entire GIT.In this study,we investigated the microbiota along the GIT of five Chinese Mongolian sheep using PCR-denaturing gradient gel electrophoresis(DGGE) and real-time PCR analysis.Results indicated that microbiota were more abundant in the stomach and large intestine than in the small intestine.DGGE and real-time PCR revealed the predominance of Firmicutes and Bacteroidetes in the GIT.Meanwhile,Ruminococcus flavefaciens and Clostridium cluster Ⅳ showed significant difference in their abundance along the GIT(P<0.05).Fibrobacter succinogenes was the most dominant species,followed by Ruminococcus albus and R.flavefaciens.The ileum harbored a larger number of cellulolytic bacteria,particlarly-Clostridium cluster Ⅳ,than reported previously.In addition,comparisons between microbiota in the rumen and rectum indicated similar number of total bacteria,Firmicutes,Bacteroidetes,F.succinogenes,Butyrivibrio fibrisolvens,Clostridium cluster Ⅳ,and Clostridium cluster ⅩⅣa,whereas the number of R.albus and R.flavefaciens was higher in the rumen.This study investigated the composition and quantification of GIT microbial community in Chinese Mongolian sheep,and revealed for the first time the cellulolytic bacterial community in these sheep.
A balanced gastrointestinal microbial ecosystem is crucial for the health and growth of animals.In the gastrointestinal tract(GIT) of ruminants,cellulolytic bacteria aid in the digestion and absorption of nutrients.Rumen contents and feces in ruminants are often used to assess gastrointestinal microbial communities;however,these sites do not guarantee to represent the diversity of microbes found in the entire GIT.In this study,we investigated the microbiota along the GIT of five Chinese Mongolian sheep using PCR-denaturing gradient gel electrophoresis(DGGE) and real-time PCR analysis.Results indicated that microbiota were more abundant in the stomach and large intestine than in the small intestine.DGGE and real-time PCR revealed the predominance of Firmicutes and Bacteroidetes in the GIT.Meanwhile,Ruminococcus flavefaciens and Clostridium cluster Ⅳ showed significant difference in their abundance along the GIT(P<0.05).Fibrobacter succinogenes was the most dominant species,followed by Ruminococcus albus and R.flavefaciens.The ileum harbored a larger number of cellulolytic bacteria,particlarly-Clostridium cluster Ⅳ,than reported previously.In addition,comparisons between microbiota in the rumen and rectum indicated similar number of total bacteria,Firmicutes,Bacteroidetes,F.succinogenes,Butyrivibrio fibrisolvens,Clostridium cluster Ⅳ,and Clostridium cluster ⅩⅣa,whereas the number of R.albus and R.flavefaciens was higher in the rumen.This study investigated the composition and quantification of GIT microbial community in Chinese Mongolian sheep,and revealed for the first time the cellulolytic bacterial community in these sheep.
A balanced gastrointestinal microbial ecosystem is crucial for the health and growth of animals.In the gastrointestinal tract(GIT) of ruminants,cellulolytic bacteria aid in the digestion and absorption of nutrients.Rumen contents and feces in ruminants are often used to assess gastrointestinal microbial communities;however,these sites do not guarantee to represent the diversity of microbes found in the entire GIT.In this study,we investigated the microbiota along the GIT of five Chinese Mongolian sheep using PCR-denaturing gradient gel electrophoresis(DGGE) and real-time PCR analysis.Results indicated that microbiota were more abundant in the stomach and large intestine than in the small intestine.DGGE and real-time PCR revealed the predominance of Firmicutes and Bacteroidetes in the GIT.Meanwhile,Ruminococcus flavefaciens and Clostridium cluster Ⅳ showed significant difference in their abundance along the GIT(P<0.05).Fibrobacter succinogenes was the most dominant species,followed by Ruminococcus albus and R.flavefaciens.The ileum harbored a larger number of cellulolytic bacteria,particlarly-Clostridium cluster Ⅳ,than reported previously.In addition,comparisons between microbiota in the rumen and rectum indicated similar number of total bacteria,Firmicutes,Bacteroidetes,F.succinogenes,Butyrivibrio fibrisolvens,Clostridium cluster Ⅳ,and Clostridium cluster ⅩⅣa,whereas the number of R.albus and R.flavefaciens was higher in the rumen.This study investigated the composition and quantification of GIT microbial community in Chinese Mongolian sheep,and revealed for the first time the cellulolytic bacterial community in these sheep.
引文
Abo El-Nor S,AbuGhazaleh AA,Potu RB,Hastings D,Khattab MSA(2010)Effects of differing levels of glycerol on rumen fermentation and bacteria.Anim Feed Sci Tech 162(3):99-105
Backhed F,Ley RE,Sonnenburg JL,Peterson DA,Gordon JI(2005)Host-bacterial mutualism in the human intestine.Science 307(5717):1915-1920
Bauer E,Williams BA,Bosch MW,Voigt C,Mosenthin R,Verstegen MW(2004)Differences in microbial activity of digesta from three sections of the porcine large intestine according to in vitro fermentation of carbohydrate-rich substrates.J Sci Food Agr 84(15):2097-2104
Backhed F,Ley RE,Sonnenburg JL,Peterson DA,Gordon JI(2005)Host-bacterial mutualism in the human intestine.Science 307(5717):1915-1920
Bauer E,Williams BA,Bosch MW,Voigt C,Mosenthin R,Verstegen MW(2004)Differences in microbial activity of digesta from three sections of the porcine large intestine according to in vitro fermentation of carbohydrate-rich substrates.J Sci Food Agr 84(15):2097-2104