PLGF对缺血损伤大脑皮层神经元凋亡及相关蛋白的影响
详细信息 查看官网全文
- 英文论文题名：Effects of PLGF on neuronal Apoptosis and associated protein in ischemic lesions
- 论文作者：盖聪 ; 孙红梅 ; 李卫红 ; 强天遥 ; 冯琬迪 ; 马浩洁 ; 柴原 ; 郭振宇 ; 王媛媛
- 英文论文作者：GAI Cong ; SUN Hong-Mei ; LI Wei-Hong ; QIANG Tian-Yao ; FENG Wan-Di ; MA Hao-Jie ; CHAI Yuan ; GUO Zhen-Yu ; WANG Yuan-Yuan ; Department of Anatomy ; Beijing university of Chinese medicine ; Beijing university of Chinese medicine
- 年：2017
- 作者机构：北京中医药大学解剖教研室;北京中医药大学;
- 论文关键词：PLGF ; 脑缺血 ; 皮层神经元 ; 凋亡
- 英文论文关键词：PLGF ; cerebral ischemia ; cortex neuron ; apoptosis
- 会议召开时间：2017-08-07
- 会议录名称：中国解剖学会第六届全国解剖学技术学术会议论文汇编
- 语种：中文
- 分类号：R743
- 学会代码：ZGJP
- 会议名称：中国解剖学会第六届全国解剖学技术学术会议
- 会议地点：中国江西九江
- 主办单位：中国解剖学会科技开发和咨询工作委员会
- 学会名称：中国解剖学会
- 页数：4
- 文件大小：1910k
- 原文格式：D
- 会议级别：全国
摘要
目的探讨胎盘生长因子(Placenta growth factor,PLGF)抗拟缺血神经元凋亡作用。方法原代大鼠大脑皮层神经元培养,采用氧糖剥夺法建立模拟体外脑缺血神经元损伤模型,PLGF干预,CCK-8法检测细胞存活率;TUNEL法检测神经元凋亡情况;western blot法检测Bax、Bcl-2的表达。结果与正常组相比,模型组神经元存活率明显降低,凋亡率明显增高,Bcl-2表达显著减低,Bax表达显著升高(p<0.01);与模型相比,PLGF组神经元存活率明显提高,凋亡率明显降低,Bcl-2表达显著升高,Bax表达显著减低(P<0.01)。结论 PLGF可能是通过调节Bax、Bcl-2蛋白表达,减少神经元凋亡,发挥保护缺血损伤神经元作用。
Objective To investigate the effect of placental growth factor PLGF on neuronal apoptosis in ischemic lesions.Methods Primary rat cerebral cortical neurons were cultured.The in vitro model of Cerebral ischemic neuronal injury induced by cerebral ischemia was established by oxygen-glucose deprivation.After PLGF intervention,CCK-8 method was used to detect the cell viability,TUNEL method was used to detect neuronal apoptosis,and the expression of Bax and Bcl-2 was detected by western blot.Results Compared with the normal group,the survival rate of the neurons in the model group was significantly decreased,the apoptosis rate was significantly increased,the expression of Bcl-2 was significantly decreased and the expression of Bax was significantly increased(P<0.01).Compared with the model group,the survival rate of PLGF group was significantly increased,the apoptosis rate was significantly decreased,Bcl-2 expression was significantly increased,Bax expression was significantly reduced(P<0.01).Conclusion PLGF may play an important role in protecting the ischemic-injured neurons by reducing neuronal apoptosis and regulating the expression of Bax and Bcl-2 protein.
Objective To investigate the effect of placental growth factor PLGF on neuronal apoptosis in ischemic lesions.Methods Primary rat cerebral cortical neurons were cultured.The in vitro model of Cerebral ischemic neuronal injury induced by cerebral ischemia was established by oxygen-glucose deprivation.After PLGF intervention,CCK-8 method was used to detect the cell viability,TUNEL method was used to detect neuronal apoptosis,and the expression of Bax and Bcl-2 was detected by western blot.Results Compared with the normal group,the survival rate of the neurons in the model group was significantly decreased,the apoptosis rate was significantly increased,the expression of Bcl-2 was significantly decreased and the expression of Bax was significantly increased(P<0.01).Compared with the model group,the survival rate of PLGF group was significantly increased,the apoptosis rate was significantly decreased,Bcl-2 expression was significantly increased,Bax expression was significantly reduced(P<0.01).Conclusion PLGF may play an important role in protecting the ischemic-injured neurons by reducing neuronal apoptosis and regulating the expression of Bax and Bcl-2 protein.
引文
[1]Martinez B,Peplow P V.Blood micro RNAs as potential diagnostic and prognostic markers in cerebral ischemic injury[J].Neural Regeneration Research(中国神经再生研究(英文版)),2016,11(9):1375-1378.
[2]盖聪,李澎涛,孙红梅,等.通心络对急性脑梗死大鼠脑组织及胎盘生长因子表达的影响[J].中医学报,2014(11):1603-1607.
[3]Chaballe L,Schoenen J,Franzen R.Placental growth factor:a tissue modelling factor with therapeutic potentials in neurology?[J].Acta Neurologica Belgica,2011,111(1):10-17.
[4]Du h,Li P,Pan Y,Li W,hou J.et al.Vascular endothelial growth factor signaling implicated in neuroprotective effects of placental growth factor in an in vitro ischemic model.brain Res.2010,1357:1-8.
[5]Dai R L,Zhu S Y,Xai Y P et al.Sonic hedgehog protects cortical neurons against oxidative stress[J].Neurochem Res.2011 Apr;36(1):67-75
[6]盖聪,李澎涛,孙红梅,等.通心络干预的脑微血管内皮细胞条件液对大鼠脑皮层神经元的影响[J].中医学报,2014(6):769-771.
[7]Maglione D,Guerriero V,Viglietto G,et al.Isolation of a human placenta c DNA coding for a protein related to the vascular permeability factor[J].Proceedings of the National Academy of Sciences,1991,88(20):9267-9271.
[8]Ribatti D.The discovery of placental growth factor and its role in angiogenesis:ahistorical review[J].Angiogenesis,2008,11(3):215-221.
[9]De Falco S,Gigante b,Persico MG,Structure and function of Placental growth factor.Trends Cardiovasc.Med.2002;12:241-6.
[10]Lecarpentieré,Vieillefosse S,Haddad B,et al.Placental growth factor(Pl GF)and s Flt-1 during pregnancy:physiology,assay and interest in preeclampsia[J].Annales De Biologie Clinique,2016,74(3):259-267.
[11]Beck H,Acker T,Püschel AW J et al.Cell type-specific expression of neuropilins in an MCA-occlusion model in mice suggests a potential role in post-ischemic brain remodeling[J].Neuropathol Exp Neurol.2002,61(4):339-350.
[12]hayashi T,noshita n,Sugawara T,Chan Ph.Temporal profile of angiogenesis and expression of related genes in the brain after ischemia.J Cerebblood Flow Metab.2003;23:166-80.
[13]Liu H,Honmou O,Harada K,et al.Neuroprotection by PLGF genemodified human mesenchymal stem cells after cerebral ischaemia.Brain.2006 Oct;129(pt10):2734-45
[14]Patel n,Kalra VK.Placenta growth factor(PLGF)-induced early growth response 1(egr-1)regulateshypoxia inducible factor-1{alpha}(h IF-1{alpha})in endothelial cells.J biol Chem.2010;285:20570-9.
[2]盖聪,李澎涛,孙红梅,等.通心络对急性脑梗死大鼠脑组织及胎盘生长因子表达的影响[J].中医学报,2014(11):1603-1607.
[3]Chaballe L,Schoenen J,Franzen R.Placental growth factor:a tissue modelling factor with therapeutic potentials in neurology?[J].Acta Neurologica Belgica,2011,111(1):10-17.
[4]Du h,Li P,Pan Y,Li W,hou J.et al.Vascular endothelial growth factor signaling implicated in neuroprotective effects of placental growth factor in an in vitro ischemic model.brain Res.2010,1357:1-8.
[5]Dai R L,Zhu S Y,Xai Y P et al.Sonic hedgehog protects cortical neurons against oxidative stress[J].Neurochem Res.2011 Apr;36(1):67-75
[6]盖聪,李澎涛,孙红梅,等.通心络干预的脑微血管内皮细胞条件液对大鼠脑皮层神经元的影响[J].中医学报,2014(6):769-771.
[7]Maglione D,Guerriero V,Viglietto G,et al.Isolation of a human placenta c DNA coding for a protein related to the vascular permeability factor[J].Proceedings of the National Academy of Sciences,1991,88(20):9267-9271.
[8]Ribatti D.The discovery of placental growth factor and its role in angiogenesis:ahistorical review[J].Angiogenesis,2008,11(3):215-221.
[9]De Falco S,Gigante b,Persico MG,Structure and function of Placental growth factor.Trends Cardiovasc.Med.2002;12:241-6.
[10]Lecarpentieré,Vieillefosse S,Haddad B,et al.Placental growth factor(Pl GF)and s Flt-1 during pregnancy:physiology,assay and interest in preeclampsia[J].Annales De Biologie Clinique,2016,74(3):259-267.
[11]Beck H,Acker T,Püschel AW J et al.Cell type-specific expression of neuropilins in an MCA-occlusion model in mice suggests a potential role in post-ischemic brain remodeling[J].Neuropathol Exp Neurol.2002,61(4):339-350.
[12]hayashi T,noshita n,Sugawara T,Chan Ph.Temporal profile of angiogenesis and expression of related genes in the brain after ischemia.J Cerebblood Flow Metab.2003;23:166-80.
[13]Liu H,Honmou O,Harada K,et al.Neuroprotection by PLGF genemodified human mesenchymal stem cells after cerebral ischaemia.Brain.2006 Oct;129(pt10):2734-45
[14]Patel n,Kalra VK.Placenta growth factor(PLGF)-induced early growth response 1(egr-1)regulateshypoxia inducible factor-1{alpha}(h IF-1{alpha})in endothelial cells.J biol Chem.2010;285:20570-9.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |