Somatosensory Neuron Types Identified by High-Coverage Single-Cell RNA-Sequencing and Functional Heterogeneity
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- 英文论文题名：Somatosensory Neuron Types Identified by High-Coverage Single-Cell RNA-Sequencing and Functional Heterogeneity
- 论文作者：李昌林 ; Kai-Cheng Li ; Dan Wu ; Yan Chen ; Hao Luo ; Jing-Rong Zhao ; Sa-Shuang Wang ; Ming-Ming Sun ; Ying-Jin Lu ; Yan-Qing Zhong ; Xu-Ye Hu ; Rui Hou ; Bei-Bei Zhou ; Lan Bao ; Hua-Sheng Xiao ; Xu Zhang
- 英文论文作者：Chang-Lin Li ; Kai-Cheng Li ; Dan Wu ; Yan Chen ; Hao Luo ; Jing-Rong Zhao ; Sa-Shuang Wang ; Ming-Ming Sun ; Ying-Jin Lu ; Yan-Qing Zhong ; Xu-Ye Hu ; Rui Hou ; Bei-Bei Zhou ; Lan Bao ; Hua-Sheng Xiao ; Xu Zhang ; Institute of Neuroscience and State Key Laboratory of Neuroscience ; CAS Center for Excellence in Brain Science ; Shanghai Institutes for Biological Sciences ; Chinese Academy of Sciences ; School of Life Science and Technology ; Shanghai Tec University ; National Engineering Center for Biochip at Shanghai ; Shanghai Clinical Center ; Chinese Academy of Sciences/Xu Hui Central Hospital ; State Key Laboratory of Cell Biology ; Institute of Biochemistry and Cell Biology ; Shanghai Institutes for Biological Sciences ; Chinese Academy of Sciences
- 年：2016
- 作者机构：Institute of Neuroscience and State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences;School of Life Science and Technology, Shanghai Tec University;National Engineering Center for Biochip at Shanghai;Shanghai Clinical Center, Chinese Academy of Sciences/Xu Hui Central Hospital;State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences;
- 会议召开时间：2016-09-24
- 会议录名称：长三角地区神经科学论坛2016暨第八次会员代表大会摘要集
- 语种：英文
- 分类号：R338
- 学会代码：ZGSK
- 会议名称：长三角地区神经科学论坛2016暨第八次会员代表大会
- 会议地点：中国上海
- 主办单位：上海市神经科学学会
- 学会名称：中国神经科学学会
- 页数：1
- 文件大小：496k
- 原文格式：D
- 会议级别：地方
摘要
Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron-typing with the sensory phenotypes. Here, we classified somatosensory neurons of the mouse dorsal root ganglion(DRG) by highcoverage single-cell RNA-sequencing(10,950 ± 1,218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli were recorded using an in vivo whole-cell patch-clamp technique and classified by neuron-type genetic markers. Small-diameter DRG neurons were classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptor(MHN). Each of the MHN types was further sorted into two subtypes. Large DRG neurons were categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors(MNs) marked by BAI1-associated protein 2-like 1(Baiap2l1) expression. Mechanoreceptors marked by trafficking protein particle complex 3-like and Baiap2/1-marked MNs were further clustered into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.
Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron-typing with the sensory phenotypes. Here, we classified somatosensory neurons of the mouse dorsal root ganglion(DRG) by highcoverage single-cell RNA-sequencing(10,950 ± 1,218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli were recorded using an in vivo whole-cell patch-clamp technique and classified by neuron-type genetic markers. Small-diameter DRG neurons were classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptor(MHN). Each of the MHN types was further sorted into two subtypes. Large DRG neurons were categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors(MNs) marked by BAI1-associated protein 2-like 1(Baiap2l1) expression. Mechanoreceptors marked by trafficking protein particle complex 3-like and Baiap2/1-marked MNs were further clustered into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.
Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron-typing with the sensory phenotypes. Here, we classified somatosensory neurons of the mouse dorsal root ganglion(DRG) by highcoverage single-cell RNA-sequencing(10,950 ± 1,218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli were recorded using an in vivo whole-cell patch-clamp technique and classified by neuron-type genetic markers. Small-diameter DRG neurons were classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptor(MHN). Each of the MHN types was further sorted into two subtypes. Large DRG neurons were categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors(MNs) marked by BAI1-associated protein 2-like 1(Baiap2l1) expression. Mechanoreceptors marked by trafficking protein particle complex 3-like and Baiap2/1-marked MNs were further clustered into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.
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