大熊猫粪便中乳酸杆菌的分离筛选与鉴定
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- 英文论文题名：Isolation and screening of lactic acid bacteria from giant panda fecal
- 论文作者：Qian Liu ; XueQin Ni ; Dong Zeng
- 英文论文作者：Qian Liu ; XueQin Ni ; Dong Zeng ; Animal Microecology Institute ; College of Veterinary Medicine ; Sichuan Agricultural University ; Key Laboratory of Animal Disease and Human Health of Sichuan Province
- 年：2016
- 作者机构：Animal Microecology Institute,College of Veterinary Medicine,Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province;
- 论文关键词：大熊猫 ; 益生菌 ; 乳酸杆菌 ; 分离 ; 筛选
- 英文论文关键词：giant panda ; probiotic ; lactic acid bacteria ; isolation ; screening
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S865.3;Q93
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：33k
- 原文格式：O
- 会议级别：全国
摘要
粪样来自于7只健康的大熊猫。本研究共分离得到了20株肠球菌和5株乳酸杆菌(E1,G83,G88,G89,G90)。通过抑菌试验发现5株乳酸杆菌都对大肠杆菌、金黄色葡萄球菌和沙门氏菌都有抑制作用;通过体外耐酸耐胆盐和药敏试验发现G83有令人满意的益生效果,进一步的16S rDNA分子生物学鉴定结果显示为植物乳杆菌。
To fecal samples collected from seven health giant pandas.This study has gained 25 strains bacteria(20 Enterococcus and 5 Lactobacillus).The strains which exhibited widest zones of inhibition against enterotoxigenic Escherichia coli,Staphylococcus aureus,Salmonella were E1,G83,G88,G89 and G90 respectively.Isolate G83 was found to possess desirable probiotic properties in vitro and futher identification based on the nucleotide sequence of its 16 S rDNA showed 99% homology to Lactobacillus plantarum.
To fecal samples collected from seven health giant pandas.This study has gained 25 strains bacteria(20 Enterococcus and 5 Lactobacillus).The strains which exhibited widest zones of inhibition against enterotoxigenic Escherichia coli,Staphylococcus aureus,Salmonella were E1,G83,G88,G89 and G90 respectively.Isolate G83 was found to possess desirable probiotic properties in vitro and futher identification based on the nucleotide sequence of its 16 S rDNA showed 99% homology to Lactobacillus plantarum.
引文