姜花中转录抑制因子HcJAZ1和HcJAZ2的克隆与功能信息学分析
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- 英文论文题名：Cloning and Functional Bioinformatics Analysis of HcJAZ1 and HcJAZ2 Gene from Hedychium coronarium
- 论文作者：付余霞 ; 李昕悦 ; 范燕萍
- 英文论文作者：FU Yu-xia ; LI Xin-yue ; FAN Yan-ping ; College of Forestry and Landscape Architecture ; Flower Research Center ; South China Agricultural University
- 年：2016
- 作者机构：华南农业大学林学与风景园林学院华南农业大学花卉研究中心;
- 论文关键词：姜花HcJAZ ; 基因克隆 ; 序列分析 ; 功能分析
- 英文论文关键词：Hedychium coronarium ; HcJAZ ; Gene cloning ; Sequence analysis ; Function analysis
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.19
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：9
- 文件大小：2263k
- 原文格式：O
- 会议级别：全国
摘要
以白姜花(Hedychium coronarium)为材料,利用RT-PCR技术克隆得到两个JA途径的转录抑制因子JAZ(Jasmonate ZIM-domain)基因,HcJAZ1和HcJAZ2。HcJAZ1基因全长1238bp,具有完整的开放阅读框708bp,编码235个氨基酸,预测分子量为25.7kDa,等电点(pI)为9.26。HcJAZ2基因全长1505bp,具有完整的开放阅读框780bp,编码259个氨基酸,预测分子量为28.5kDa,等电点(pI)为7.62。蛋白家族结构域分析显示HcJAZ1和HcJAZ2蛋白序列均含有和JAZs形成同源或者异源二聚体相关的高度保守的TIFY(又称ZIM)结构域,以及和泛素化降解,核定位相关的CCT_1(又称Jas)结构域。氨基酸同源序列比对结果表明不同的JAZ基因氨基酸序列的变化较小。聚类分析进化树结果表明,HcJAZ1和HcJAZ2与拟南芥的JAZ1和AtJAZ2亲缘关系相对最近,且具有较高的同源性。核定位信号分析预测结果显示HcJAZ1和HcJAZ2均在N端存在一段潜在核定位信号,NLS预测值分别为3.4和3.1。蛋白疏水性预测分析结果表明HcJAZ1,HcJAZ2均有较强的疏水性。二级结构预测分析结果显示HcJAZ1含有68.5%成α螺旋残基,37.9%成β折叠残基,15.7%成β转角残基;HcJAZ2含有56.9%成理螺旋残基,41.9%成β折叠残基,17.7%成β转角残基,这和AtJAZ1,AtJAZ2的二级结构表现出较高的相似性。此外,蛋白三维建模分析显示TIFY结构域和CCT_1结构域的空间位置关系在HcJAZ1和HcJAZ2中较为相似。
A transcription inhibitors gene,named HcJAZl and HcJAZ2,was isolated from the flowers of H.coronarium using the gene specific primers which obtained according to HcJAZ sequence by the method of RT-PCR technology.The HcJAZl gene was 1238 bp in length,containing a 708 bp ORF which encoded 235 amino acid residues of 25.7 kDa molecular weighth.The HcJAZ2 gene was 1505 bp in length,containing a 780 bp ORF which encoded 259 amino acid residues of 28.5 kDa molecular weighth.Protein predicted domain indicated that HcJAZl and HcJAZl have the typcial high conserved site TIFY domain which can form homodimer itself or heterodimers with other receptors and CCT_ 1 motif which can bind other transcripton factors including COIl.The result of amino acid sequence alignment reveals that the protein HcJAZl and HcJAZ2 poss some kind of the typical TIFY domain and CCT_ 1 motif of JAZ gene families compared to the JAZ in arabidopsis thaliana.Phylogenetic tree analysis for AtJAZl-12 as well as HcJAZl and HcJAZ2 indicates that AtJAZ1 and AtJAZ2 are the ones most closely related to HcJAZl and HcJAZ2.Subcellular localization shows the NLS scores of the HcJAZl and HcJAZ2 is 3.4 and 3.1,respectively.The prediction of the hydrophobic shows that HcJAZl and HcJAZ2 are high lyophobic.The secondary structure prediction results manifests that HcJAZl has 68.5%α-helix,37.9%p-sheet,15.7%β-turn;HcJAZ2 has 56.9%α-helix,41.9%13-sheet,17.7%p-turn,it is highly similar with what in AtJAZl and AtJAZ2.Besides,the results of the 3D structure prediction shows the similar special relationship between TIFY domain and CCT_ 1 domain in both HcJAZl and HcJAZ2.
A transcription inhibitors gene,named HcJAZl and HcJAZ2,was isolated from the flowers of H.coronarium using the gene specific primers which obtained according to HcJAZ sequence by the method of RT-PCR technology.The HcJAZl gene was 1238 bp in length,containing a 708 bp ORF which encoded 235 amino acid residues of 25.7 kDa molecular weighth.The HcJAZ2 gene was 1505 bp in length,containing a 780 bp ORF which encoded 259 amino acid residues of 28.5 kDa molecular weighth.Protein predicted domain indicated that HcJAZl and HcJAZl have the typcial high conserved site TIFY domain which can form homodimer itself or heterodimers with other receptors and CCT_ 1 motif which can bind other transcripton factors including COIl.The result of amino acid sequence alignment reveals that the protein HcJAZl and HcJAZ2 poss some kind of the typical TIFY domain and CCT_ 1 motif of JAZ gene families compared to the JAZ in arabidopsis thaliana.Phylogenetic tree analysis for AtJAZl-12 as well as HcJAZl and HcJAZ2 indicates that AtJAZ1 and AtJAZ2 are the ones most closely related to HcJAZl and HcJAZ2.Subcellular localization shows the NLS scores of the HcJAZl and HcJAZ2 is 3.4 and 3.1,respectively.The prediction of the hydrophobic shows that HcJAZl and HcJAZ2 are high lyophobic.The secondary structure prediction results manifests that HcJAZl has 68.5%α-helix,37.9%p-sheet,15.7%β-turn;HcJAZ2 has 56.9%α-helix,41.9%13-sheet,17.7%p-turn,it is highly similar with what in AtJAZl and AtJAZ2.Besides,the results of the 3D structure prediction shows the similar special relationship between TIFY domain and CCT_ 1 domain in both HcJAZl and HcJAZ2.
引文
1.李瑞红,范燕萍.2007.白姜花不同开花时期的香味组分及其变化[J].植物生理学通讯,01:176-180.
2.蒋科技,皮妍,侯嵘,等.2010.植物内源茉莉酸类物质的生物合成途径及其生物学意义[J].植物学报,02:137-148.
3.王台,钱前,袁明,等.2010.2009年中国植物科学若干领域重要研究进展[J].植物学报,45(3):265-306.
4.Acosta I F,E E Farmer.2010.Jasmonates.The Arabidopsis book/American Society of Plant Biologists,8;e0129.
5.Bai Y,Y Meng,D Huang,et al.2011.Origin and evolutionary analysis of the plant-specific TIFY transcription factor family[J].Genomics,98(2):128-136.
6.Chini A,M Boter,R Solano.2009.Plant oxylipins;COIl/JAZs/MYC2 as the core jasmonic acid-signalling module[J].Febs Journal,276(17):4682-4692.
7.Chini A,S Fonseca,G Fernandez,et al.2007.The JAZ family of repressors is the missing link in jasmonate signalling[J].Nature,448(7154):666-671.
8.Chung H S,G A Howe.2009.A critical role for the TIFY motif in repression of jasmonate signaling by a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10in Arabidopsis[J].The Plant Cell,21(1):131-145.
9.Demianski A J,K M Chung,B N Kunkel.2012.Analysis of Arabidopsis JAZ gene expression during Pseudomonas syringae pathogenesis[J].Molecular plant pathology,13(1):46-57.
10.Derelle E,C Ferraz,S Rombauts,et al.2006.Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features[J].Proceedings of the National Academy of Sciences,103(31):11647-11652.
11.Grechkin A.1998.Recent developments in biochemistry of the plant lipoxygenase pathway[J].Progress in Lipid Research,37(5):317-352.
12.Grunewald W,B Vanholme,L Pauwels,et al.2009.Expression of the Arabidopsis jasmonate signalling repressor JAZ1/TIFY10A is stimulated by auxin[J].EMBO reports,10(8):923-928.
13.Katsir L,H S Chung,A J Koo,et al.2008.Jasmonate signaling:a conserved mechanism of hormone sensing[J].Current opinion in plant biology,11(4):428-435.
14.Kazan K,J M Manners.2012.JAZ repressors and the orchestration of phytohormone crosstalk[J].Trends in plant science,17(1):22-31.
15.Melotto M,C Mecey,Y Niu,et al.2008.A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine-and jasmonoyl isoleucine-dependent interactions with the COI1 F-box protein[J].The Plant Journal,55(6):979-988.
16.Memelink J.2009.Regulation of gene expression by jasmonate hormones[J].Phytochemistry,70(13):1560-1570.
17.Navarro L,R Bari,P Achard,et al.2008.DELLAs control plant immune responses by modulating the balance of jasmonic acid and salicylic acid signaling[J].Current Biology,18(9):650-655.
18.Perez A C,A Goossens.2013.Jasmonate signalling;a copycat of auxin signalling?[J].Plant,cell&environment,36(12):2071-2084.
19.Shan X,J Yan,D Xie.2012.Comparison of phytohormone signaling mechanisms[J].Current opinion in plant biology,15(1):84-91.
20.Staswick P E.2008.JAZing up jasmonate signaling[J].Trends in plant science,13(2):66-71.
21.Thines B,L Katsir,M Melotto,et aL 2007.JAZ repressor proteins are targets of the SCFC0I1 complex during jasmonate signalling[J].Nature,448(7154):661-665.
22.Vanholme B,W Grunewald,A Bateman,et al.2007.The tify family previously known as ZIM[J].Trends in plant science,12(6):239-244.
23.Wasternack C.2014.Perception,signaling and cross-talk of jasmonates and the seminal contributions of the Daoxin Xie's lab and the Chuanyou Li's lab[J].Plant cell reports,33(5):707-718.
24.Yan J,C Zhang,M Gu,et al.2009.The Arabidopsis CORONATINE INSENSmVEl protein is a jasmonate receptor[J].The Plant Cell,21(8):2220-2236.
25.Yan Y,S Stolz,A Chetelat,et al.2007.A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway[J].The Plant Cell,19(8):2470-2483.
26.Yang M,W S May,T Ito.1999.JAZ requires the doublestranded RNA-binding zinc finger motifs for nuclear localization[J].Journal of Biological Chemistry,274(39):27399-27406.
27.Li Rui-hong,Fan Yan-ping.2007.Changes in floral aroma constituents in Hedychium coronarium Koening during different blooming stages[J].Plant physiology communications,(01):176-180.
28.Wang Tai,Qian qian,Yuan Ming,et al.2010.Scientific development in several aera of Chinese Botony[J].Chinese Bulletin of Botonay,45(3):265-306.
29.Jiang Ke-ji Pi Yan,Hou Rong,et al.2010.Jasmonate Biosynthetic Pathway:Its Physiological Role and Potential Application in Plant Secondary Metabolic Engineering[J].Chinese Bulletin of Botonay.(02):137-148.
2.蒋科技,皮妍,侯嵘,等.2010.植物内源茉莉酸类物质的生物合成途径及其生物学意义[J].植物学报,02:137-148.
3.王台,钱前,袁明,等.2010.2009年中国植物科学若干领域重要研究进展[J].植物学报,45(3):265-306.
4.Acosta I F,E E Farmer.2010.Jasmonates.The Arabidopsis book/American Society of Plant Biologists,8;e0129.
5.Bai Y,Y Meng,D Huang,et al.2011.Origin and evolutionary analysis of the plant-specific TIFY transcription factor family[J].Genomics,98(2):128-136.
6.Chini A,M Boter,R Solano.2009.Plant oxylipins;COIl/JAZs/MYC2 as the core jasmonic acid-signalling module[J].Febs Journal,276(17):4682-4692.
7.Chini A,S Fonseca,G Fernandez,et al.2007.The JAZ family of repressors is the missing link in jasmonate signalling[J].Nature,448(7154):666-671.
8.Chung H S,G A Howe.2009.A critical role for the TIFY motif in repression of jasmonate signaling by a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10in Arabidopsis[J].The Plant Cell,21(1):131-145.
9.Demianski A J,K M Chung,B N Kunkel.2012.Analysis of Arabidopsis JAZ gene expression during Pseudomonas syringae pathogenesis[J].Molecular plant pathology,13(1):46-57.
10.Derelle E,C Ferraz,S Rombauts,et al.2006.Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features[J].Proceedings of the National Academy of Sciences,103(31):11647-11652.
11.Grechkin A.1998.Recent developments in biochemistry of the plant lipoxygenase pathway[J].Progress in Lipid Research,37(5):317-352.
12.Grunewald W,B Vanholme,L Pauwels,et al.2009.Expression of the Arabidopsis jasmonate signalling repressor JAZ1/TIFY10A is stimulated by auxin[J].EMBO reports,10(8):923-928.
13.Katsir L,H S Chung,A J Koo,et al.2008.Jasmonate signaling:a conserved mechanism of hormone sensing[J].Current opinion in plant biology,11(4):428-435.
14.Kazan K,J M Manners.2012.JAZ repressors and the orchestration of phytohormone crosstalk[J].Trends in plant science,17(1):22-31.
15.Melotto M,C Mecey,Y Niu,et al.2008.A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine-and jasmonoyl isoleucine-dependent interactions with the COI1 F-box protein[J].The Plant Journal,55(6):979-988.
16.Memelink J.2009.Regulation of gene expression by jasmonate hormones[J].Phytochemistry,70(13):1560-1570.
17.Navarro L,R Bari,P Achard,et al.2008.DELLAs control plant immune responses by modulating the balance of jasmonic acid and salicylic acid signaling[J].Current Biology,18(9):650-655.
18.Perez A C,A Goossens.2013.Jasmonate signalling;a copycat of auxin signalling?[J].Plant,cell&environment,36(12):2071-2084.
19.Shan X,J Yan,D Xie.2012.Comparison of phytohormone signaling mechanisms[J].Current opinion in plant biology,15(1):84-91.
20.Staswick P E.2008.JAZing up jasmonate signaling[J].Trends in plant science,13(2):66-71.
21.Thines B,L Katsir,M Melotto,et aL 2007.JAZ repressor proteins are targets of the SCFC0I1 complex during jasmonate signalling[J].Nature,448(7154):661-665.
22.Vanholme B,W Grunewald,A Bateman,et al.2007.The tify family previously known as ZIM[J].Trends in plant science,12(6):239-244.
23.Wasternack C.2014.Perception,signaling and cross-talk of jasmonates and the seminal contributions of the Daoxin Xie's lab and the Chuanyou Li's lab[J].Plant cell reports,33(5):707-718.
24.Yan J,C Zhang,M Gu,et al.2009.The Arabidopsis CORONATINE INSENSmVEl protein is a jasmonate receptor[J].The Plant Cell,21(8):2220-2236.
25.Yan Y,S Stolz,A Chetelat,et al.2007.A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway[J].The Plant Cell,19(8):2470-2483.
26.Yang M,W S May,T Ito.1999.JAZ requires the doublestranded RNA-binding zinc finger motifs for nuclear localization[J].Journal of Biological Chemistry,274(39):27399-27406.
27.Li Rui-hong,Fan Yan-ping.2007.Changes in floral aroma constituents in Hedychium coronarium Koening during different blooming stages[J].Plant physiology communications,(01):176-180.
28.Wang Tai,Qian qian,Yuan Ming,et al.2010.Scientific development in several aera of Chinese Botony[J].Chinese Bulletin of Botonay,45(3):265-306.
29.Jiang Ke-ji Pi Yan,Hou Rong,et al.2010.Jasmonate Biosynthetic Pathway:Its Physiological Role and Potential Application in Plant Secondary Metabolic Engineering[J].Chinese Bulletin of Botonay.(02):137-148.
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