OAZI-1 enhances the immunogenicity of Melan-A as tumor vaccine
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- 英文论文题名：OAZI-1 enhances the immunogenicity of Melan-A as tumor vaccine
- 论文作者：Wu Hongyan ; Sun Peng ; Wang Yanlin
- 英文论文作者：Wu Hongyan ; Sun Peng ; Wang Yanlin ; Department of Immunology ; Medical College of Three Gorges University ; Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy ; Medical College of Three Gorges University
- 年：2016
- 作者机构：Department of Immunology,Medical College of Three Gorges University;Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy,Medical College of Three Gorges University;
- 英文论文关键词：OAZI-1 ; Melan-A ; tumor ; immunogenicity
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R739.5
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：1291k
- 原文格式：D
- 会议级别：全国
摘要
Objective: To investigate whether ornithine decarboxylase antizyme inhibitor-1(OAZI-1) can enhance the immunogenicity of Melan-A and induce antitumor immune effect in the experimental animals or not. Methods: The eukaryotic expression plasmid pcDNA3.1(-) /OAZI-1, pcDNA3.1(-)/Melan-A and pcDNA3.1(-)/ MelanA-OAZI-1 were constructed and used to immune BALB/c mice. The spleen lymphocytes were prepared from the immunized mice and then used to determine the lymphocyte subsets by flow cytometry assay and tumor-killing activity by LDH release assay. The blood samples were collected from the immunized mice and used to test the serum INF-γ levels by ELISA.Results: The eukaryotic expression plasmid pcDNA3.1(-)/OAZI-1, pcDNA3.1(-)/Melan-A and pcDNA3.1(-)/ MelanA-OAZI-1 were constructed successfully. The results of flow cytometry assay show that all three gene vaccines could increase CD8~+ T cell ratio, among of them, the ratio in the pcDNA3.1(-)/Melan-A-OAZI-1 and pcDNA3.1(-)/Melan-A immune groups increased more significantly than other groups but no obvious differences was observed between these two groups. Similarly, all three gene vaccines could also increase CD8~+T cells ratio significantly, but, comparing with all other groups, the highest increase was observed in the pcDNA3.1(-)/Melan-A-OAZI-1 immune group(p<0.05). The LDH release analysis demonstrated that the pcDNA3.1(-)/Melan-A-OAZI-1 gene vaccines could significantly increase cytotoxic activity of the spleen lymphocyte in the immune mice,(p<0.05). The results of ELISA showed that, among the three gene vaccines only pcDNA3.1(-)/Melan-A-OAZI-1 could significantly increase the INF–γ level in the mice serum(p<0.05). Conclusion: OAZI-1 can increase antitumor immunity by promoting tumor antigen presentation.
Objective: To investigate whether ornithine decarboxylase antizyme inhibitor-1(OAZI-1) can enhance the immunogenicity of Melan-A and induce antitumor immune effect in the experimental animals or not. Methods: The eukaryotic expression plasmid pc DNA3.1(-) /OAZI-1, pc DNA3.1(-)/Melan-A and pc DNA3.1(-)/ MelanA-OAZI-1 were constructed and used to immune BALB/c mice. The spleen lymphocytes were prepared from the immunized mice and then used to determine the lymphocyte subsets by flow cytometry assay and tumor-killing activity by LDH release assay. The blood samples were collected from the immunized mice and used to test the serum INF-γ levels by ELISA.Results: The eukaryotic expression plasmid pc DNA3.1(-)/OAZI-1, pc DNA3.1(-)/Melan-A and pc DNA3.1(-)/ MelanA-OAZI-1 were constructed successfully. The results of flow cytometry assay show that all three gene vaccines could increase CD4+ T cell ratio, among of them, the ratio in the pc DNA3.1(-)/Melan-A-OAZI-1 and pc DNA3.1(-)/Melan-A immune groups increased more significantly than other groups but no obvious differences was observed between these two groups. Similarly, all three gene vaccines could also increase CD8+T cells ratio significantly, but, comparing with all other groups, the highest increase was observed in the pc DNA3.1(-)/Melan-A-OAZI-1 immune group(p<0.05). The LDH release analysis demonstrated that the pc DNA3.1(-)/Melan-A-OAZI-1 gene vaccines could significantly increase cytotoxic activity of the spleen lymphocyte in the immune mice,(p<0.05). The results of ELISA showed that, among the three gene vaccines only pc DNA3.1(-)/Melan-A-OAZI-1 could significantly increase the INF–γ level in the mice serum(p<0.05). Conclusion: OAZI-1 can increase antitumor immunity by promoting tumor antigen presentation.
Objective: To investigate whether ornithine decarboxylase antizyme inhibitor-1(OAZI-1) can enhance the immunogenicity of Melan-A and induce antitumor immune effect in the experimental animals or not. Methods: The eukaryotic expression plasmid pc DNA3.1(-) /OAZI-1, pc DNA3.1(-)/Melan-A and pc DNA3.1(-)/ MelanA-OAZI-1 were constructed and used to immune BALB/c mice. The spleen lymphocytes were prepared from the immunized mice and then used to determine the lymphocyte subsets by flow cytometry assay and tumor-killing activity by LDH release assay. The blood samples were collected from the immunized mice and used to test the serum INF-γ levels by ELISA.Results: The eukaryotic expression plasmid pc DNA3.1(-)/OAZI-1, pc DNA3.1(-)/Melan-A and pc DNA3.1(-)/ MelanA-OAZI-1 were constructed successfully. The results of flow cytometry assay show that all three gene vaccines could increase CD4+ T cell ratio, among of them, the ratio in the pc DNA3.1(-)/Melan-A-OAZI-1 and pc DNA3.1(-)/Melan-A immune groups increased more significantly than other groups but no obvious differences was observed between these two groups. Similarly, all three gene vaccines could also increase CD8+T cells ratio significantly, but, comparing with all other groups, the highest increase was observed in the pc DNA3.1(-)/Melan-A-OAZI-1 immune group(p<0.05). The LDH release analysis demonstrated that the pc DNA3.1(-)/Melan-A-OAZI-1 gene vaccines could significantly increase cytotoxic activity of the spleen lymphocyte in the immune mice,(p<0.05). The results of ELISA showed that, among the three gene vaccines only pc DNA3.1(-)/Melan-A-OAZI-1 could significantly increase the INF–γ level in the mice serum(p<0.05). Conclusion: OAZI-1 can increase antitumor immunity by promoting tumor antigen presentation.
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