Stachyose is a Preferential Carbon Source Utilized by the Rice False Smut pathogen,Ustilaginoidea virens
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- 英文论文题名：Stachyose is a Preferential Carbon Source Utilized by the Rice False Smut pathogen,Ustilaginoidea virens
- 论文作者：WANG Yu-qiu ; LI Guo-bang ; GONG Zhi-you ; LI Yan ; HUANG Fu ; FAN Jing ; WANG Wen-ming
- 英文论文作者：WANG Yu-qiu ; LI Guo-bang ; GONG Zhi-you ; LI Yan ; HUANG Fu ; FAN Jing ; WANG Wen-ming ; Rice Research Institute ; Sichuan Agricultural University at Wenjiang
- 年：2016
- 作者机构：Rice Research Institute,Sichuan Agricultural University at Wenjiang;
- 英文论文关键词：Major facilitator superfamily ; oligosaccharide ; rice false smut ; stachyose ; Villosiclava virens
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.111.4
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：43k
- 原文格式：O
- 会议级别：全国
摘要
Ustilaginoidea virens(Cooke) Tak.is the causal pathogen of rice false smut(RFS) disease.RFS is an emerging panicle disease,threatening rice production worldwide.However,the biology and pathogenicity of U.virens are still not well-understood.In this study,we found that U.virens preferentially utilize stachyose over sucrose that is previously reported to be the best carbon source for U.virens.Stachyose could promote conidia germination,hyphae elongation and mycelium growth of U.virens.Transcriptome and qRT-PCR analyses showed that genes involved in transporting carbohydrate,amino acid,inorganic ion and secondary metabolites were mostly up-regulated by stachyose than by sucrose.Moreover,Uv8b_6977 encoding a putative major facilitator superfamily(MFS) transporter was highly and specifically induced by stachyose,indicating that it may be a stachyose transporter.Expression levels of several stachyose-inducible MFS genes were also highly induced in U.wrens-infected rice spikelets especially when early false smut balls appeared,implicating that these genes may play important roles in the formation of false smut balls.Collectively,this work identifies stachyose as a preferential carbon source for U.virens,and provides gene candidates to be investigated for clarifying sugar utilization mechanism of U.virens.
Ustilaginoidea virens(Cooke) Tak.is the causal pathogen of rice false smut(RFS) disease.RFS is an emerging panicle disease,threatening rice production worldwide.However,the biology and pathogenicity of U.virens are still not well-understood.In this study,we found that U.virens preferentially utilize stachyose over sucrose that is previously reported to be the best carbon source for U.virens.Stachyose could promote conidia germination,hyphae elongation and mycelium growth of U.virens.Transcriptome and qRT-PCR analyses showed that genes involved in transporting carbohydrate,amino acid,inorganic ion and secondary metabolites were mostly up-regulated by stachyose than by sucrose.Moreover,Uv8b_6977 encoding a putative major facilitator superfamily(MFS) transporter was highly and specifically induced by stachyose,indicating that it may be a stachyose transporter.Expression levels of several stachyose-inducible MFS genes were also highly induced in U.wrens-infected rice spikelets especially when early false smut balls appeared,implicating that these genes may play important roles in the formation of false smut balls.Collectively,this work identifies stachyose as a preferential carbon source for U.virens,and provides gene candidates to be investigated for clarifying sugar utilization mechanism of U.virens.
Ustilaginoidea virens(Cooke) Tak.is the causal pathogen of rice false smut(RFS) disease.RFS is an emerging panicle disease,threatening rice production worldwide.However,the biology and pathogenicity of U.virens are still not well-understood.In this study,we found that U.virens preferentially utilize stachyose over sucrose that is previously reported to be the best carbon source for U.virens.Stachyose could promote conidia germination,hyphae elongation and mycelium growth of U.virens.Transcriptome and qRT-PCR analyses showed that genes involved in transporting carbohydrate,amino acid,inorganic ion and secondary metabolites were mostly up-regulated by stachyose than by sucrose.Moreover,Uv8b_6977 encoding a putative major facilitator superfamily(MFS) transporter was highly and specifically induced by stachyose,indicating that it may be a stachyose transporter.Expression levels of several stachyose-inducible MFS genes were also highly induced in U.wrens-infected rice spikelets especially when early false smut balls appeared,implicating that these genes may play important roles in the formation of false smut balls.Collectively,this work identifies stachyose as a preferential carbon source for U.virens,and provides gene candidates to be investigated for clarifying sugar utilization mechanism of U.virens.
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