日粮中添加枯草芽孢杆菌B10对高脂日粮诱导的小鼠脂肪代谢及抗氧化的影响
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- 英文论文题名：The effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice
- 论文作者：Kai LEI ; Ya-li LI ; Yang WANG ; Jing WEN ; Hong-zhao WU ; Dong-you YU ; Wei-fen LI
- 英文论文作者：Kai LEI ; Ya-li LI ; Yang WANG ; Jing WEN ; Hong-zhao WU ; Dong-you YU ; Wei-fen LI ; Key Laboratory of Molecular Animal Nutrition of Ministry of Education ; Institute of Feed Science ; College of Animal Science ; Zhejiang University
- 年：2016
- 作者机构：Key Laboratory of Molecular Animal Nutrition of Ministry of Education,Institute of Feed Science,College of Animal Science,Zhejiang University;
- 论文关键词：枯草芽孢杆菌 ; 高脂日粮 ; 氧化应激 ; 脂肪代谢
- 英文论文关键词：Bacillus subtilis ; High-Fat Diet ; Oxidative Stress ; Lipid Metabolism
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S816
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：83k
- 原文格式：O
- 会议级别：全国
摘要
探究枯草芽孢杆菌B10对高脂日粮诱导的小鼠的脂肪代谢及氧化应激的改善作用,并初步探讨其作用机制。将C57BL/6J雄鼠分为对照组(饲喂高脂日粮)和实验组(饲喂添加有枯草芽孢杆菌菌粉的高脂日粮)。饲喂30天后,收集小鼠的血清及肝脏样品。采用试剂盒测定抗氧化及脂肪代谢相关指标和肝脏中8-OHdG含量。使用荧光定量PCR测定小鼠肝脏中脂肪代谢和氧化应激相关基因的表达水平。饲喂含有枯草芽孢杆菌B10的高脂日粮能够有效降低小鼠的体重,减少血糖含量,降低血清中三油酸甘油酯含量及谷草转氨酶和谷丙转氨酶活力;下调肝脏中脂肪合成相关基因表达量,但上调脂肪分解相关基因表达量,并提高肝脏中抗氧化相关基因表达量。综上所述,枯草芽孢杆菌B10能有效调节小鼠脂肪代谢,并改善其氧化应激。
While a high-fat diet(HFD) is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity,probiotics are believed to have positive effects on the regulation of HFD-induced obesity as well as lipid metabolism,energy homeostasis and anti-oxidation.Because Bacillus subtilis B10 has beneficial effects on the abnormal lipid metabolism and the oxidative stress in high-fat diet-induced obese mice,ICR mice were randomly assigned into an HFD group and the HFD was supplemented with 0.1%Bacillus Subtilis BIO(HFD +B10 group).Thereafter,30 days' treatments were run,and then hepatic lipid level and antioxidant status were measured.The genes expression-related lipid metabolism and oxidative stress in the liver were determined by reverse-transcription quantitative PCR(RT-qPCR).We found that high-fat diet-induced obese mice treated with BIO showed a decrease in weight gain,serum glucose activity as well as hepatic triglyceride(TG),glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT)activity.In addition,the gene expression of antioxidant genes,glutathione reductase(GR),xanthine oxidase(XO),heat-shock protein 90(HSP90) and lipid synthesis gene 3-P-hydro-xysteroid-24 reductase(DHCR24) in the HFD+B10group were down-regulated,suggesting alleviation of oxidative stress,while the lipolysis gene3-hydroxy-3-methylglutaryl-CoA synthase 2(HMGCS2),energy metabolism gene peroxisome proliferator-activated receptor a(PPARa) and the gene encoding tumor-suppressor protein p53 were up-regulated.The regulatory and positive effect of dietary supplementation of probiotic BIO induced high-fat diet in the serum and liver of obese mice suggests that it has a beneficial effect on the homeostasis of the lipid metabolism and in alleviating oxidative stress in high-fat diet-induced obese mice.
While a high-fat diet(HFD) is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity,probiotics are believed to have positive effects on the regulation of HFD-induced obesity as well as lipid metabolism,energy homeostasis and anti-oxidation.Because Bacillus subtilis B10 has beneficial effects on the abnormal lipid metabolism and the oxidative stress in high-fat diet-induced obese mice,ICR mice were randomly assigned into an HFD group and the HFD was supplemented with 0.1%Bacillus Subtilis BIO(HFD +B10 group).Thereafter,30 days' treatments were run,and then hepatic lipid level and antioxidant status were measured.The genes expression-related lipid metabolism and oxidative stress in the liver were determined by reverse-transcription quantitative PCR(RT-qPCR).We found that high-fat diet-induced obese mice treated with BIO showed a decrease in weight gain,serum glucose activity as well as hepatic triglyceride(TG),glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT)activity.In addition,the gene expression of antioxidant genes,glutathione reductase(GR),xanthine oxidase(XO),heat-shock protein 90(HSP90) and lipid synthesis gene 3-P-hydro-xysteroid-24 reductase(DHCR24) in the HFD+B10group were down-regulated,suggesting alleviation of oxidative stress,while the lipolysis gene3-hydroxy-3-methylglutaryl-CoA synthase 2(HMGCS2),energy metabolism gene peroxisome proliferator-activated receptor a(PPARa) and the gene encoding tumor-suppressor protein p53 were up-regulated.The regulatory and positive effect of dietary supplementation of probiotic BIO induced high-fat diet in the serum and liver of obese mice suggests that it has a beneficial effect on the homeostasis of the lipid metabolism and in alleviating oxidative stress in high-fat diet-induced obese mice.
引文
An,H.M.,Park,S.Y.,Lee,D.K.,et ah,2011.Antiobesity and lipid-lowering effects of Bifidobacterium spp.in high fat diet-induced obese rats.Lipids Health Dis.10(116).[doi:10.1186/1476-511X-10-116]