猪血IgG的提取及其对仔猪生产性能和血液指标的影响
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摘要
本研究主要包括三部分：
(1)分别采用辛酸沉淀法和饱和硫酸铵盐析法从猪血浆中提取IgG，探讨提取IgG的有效方法，旨在为生产中大规模从猪血中提取IgG提供方法依据。结果显示，经改进后的辛酸沉淀法是提取高浓度、活性高，成本低IgG的有效方法。
(2)将提取IgG按不同剂量(0mg、300mg、600mg、900mg)饲喂10窝1日龄仔猪80头(每试验组20头，由每窝选1公1母计2头组成)，分别测定不同试验组仔猪平均日增重、腹泻头次、血清碱性磷酸酶活性、血清总蛋白含量、血清IgG浓度、血液T淋巴细胞百分率，旨在探明新生仔猪吸收IgG能力及外源IgG对仔猪生产性能及机体免疫力的影响。结果显示，高剂量IgG(900mg)试验组0-15日龄个体日增重、血清总蛋白含量、5日龄血清IgG浓度均显著高于对照组(0mg)(p＜0.05)，高剂量IgG(900mg)试验组5、10日龄血清碱性磷酸酶活性极显著高于对照组(p＜0.01)，并能有效降低仔猪腹头次。结果表明1日龄仔猪IgG吸收能力强，在自然哺乳基础上饲喂新生仔猪900mg的猪血来源IgG能提高生产性能以及血液生化、免疫指标。
(3)将提取IgG按不同剂量(0mg、300mg、600mg、900mg)注射10窝21日龄仔猪80头，试验分组与测定项目同(2)。结果显示，高剂量IgG(900mg)试验组个体21-30日龄平均日增重、22日龄血清总蛋白含量均显著高于对照组(p＜0.05)，并能有效降低仔猪腹头次。结果表明21日龄仔猪注射900mg猪血IgG能提高仔猪生产性能和免疫机能。
根据试验(2)、(3)结果，发现仔猪的血清碱性磷酸酶随日龄的增加而呈明显的下降趋势，同时也验证了仔猪T淋巴细胞百分率随仔猪日龄的增加呈上升趋势，从而为仔猪免疫系统发育的研究提供重要的试验数据。
Three parts were included in this study:
1. Procedure of salting out in ammonium sulfate and procedure of caprylic acid depositting were used to extract IgG from porcine blood respectively in order to compare two methods and offer the foundation for making the better into practice. The results suggest that IgG extracted by procedure of caprylic acid deposition and purified by improved technique is more practical and feasible for its high concentration and activity and low production cost.
2. Eighty one-day-old piglets from 10 litters were randomly divided into four groups fed with different doses (0mg, 300mg, 600mg and 900mg) of IgG extracted respectively. Each animal's average-day-gain(ADG), diarrhoea days, serum Alkline phosphatase (AKP) activity and total protein and IgG, and percentage of T-lymphocyte cells of blood were measured on day 5, 10, and 15 after feeding. The experiment aims at evaluating newborn piglets' ability to absorb exogenous IgG and estimate exogenous IgG's influence on piglets' performance and immune capacity. The results showed that the group fed with high dose IgG (900mg) are higher than the contrast group in ADG between day 0 and 15, both serum AKP activity and total protein on day 5 and 10, and serum IgG concentration on day 5 significantly(p<0.05). At the same time, the high dose IgG can decrease the number of diarrhoea piglets and the diarrhoea duration of piglets. It can be concluded that one-day-old piglets have high ability to absorb
exogenous IgG and feeding newborn piglets with 900mg IgG on the basis of nature lactation can improve their performance and boichemical and immune parameters of blood.
3. Eighty 21-day-old piglets from another 10 litters were evenly divided into four groups injected with different doses (Omg, 300mg, 600mg and 900mg) of IgG extracted respectively, and the same parameters as part 2 were measured. The results showed that the group injected with high dosage of IgG (900mg) are better than the contrast group in ADG between day 21 and 30 and serum total protein significantly (p<0.05). Meanwhile, the number and duration of diarrhoea piglets are less than that of the contrast. Conclusion can be made that 21-day-old piglets injected with 900mg IgG from porcine blood have high performance and immunity.
According to results of part 2 and 3, quantity of AKP in piglets' blood has a decline trend with days increasing. On the contrary, T-lymphocyte cells show an increasing trend, which provide an important experimental data for future study on immune system development of piglet.
(1)分别采用辛酸沉淀法和饱和硫酸铵盐析法从猪血浆中提取IgG，探讨提取IgG的有效方法，旨在为生产中大规模从猪血中提取IgG提供方法依据。结果显示，经改进后的辛酸沉淀法是提取高浓度、活性高，成本低IgG的有效方法。
(2)将提取IgG按不同剂量(0mg、300mg、600mg、900mg)饲喂10窝1日龄仔猪80头(每试验组20头，由每窝选1公1母计2头组成)，分别测定不同试验组仔猪平均日增重、腹泻头次、血清碱性磷酸酶活性、血清总蛋白含量、血清IgG浓度、血液T淋巴细胞百分率，旨在探明新生仔猪吸收IgG能力及外源IgG对仔猪生产性能及机体免疫力的影响。结果显示，高剂量IgG(900mg)试验组0-15日龄个体日增重、血清总蛋白含量、5日龄血清IgG浓度均显著高于对照组(0mg)(p＜0.05)，高剂量IgG(900mg)试验组5、10日龄血清碱性磷酸酶活性极显著高于对照组(p＜0.01)，并能有效降低仔猪腹头次。结果表明1日龄仔猪IgG吸收能力强，在自然哺乳基础上饲喂新生仔猪900mg的猪血来源IgG能提高生产性能以及血液生化、免疫指标。
(3)将提取IgG按不同剂量(0mg、300mg、600mg、900mg)注射10窝21日龄仔猪80头，试验分组与测定项目同(2)。结果显示，高剂量IgG(900mg)试验组个体21-30日龄平均日增重、22日龄血清总蛋白含量均显著高于对照组(p＜0.05)，并能有效降低仔猪腹头次。结果表明21日龄仔猪注射900mg猪血IgG能提高仔猪生产性能和免疫机能。
根据试验(2)、(3)结果，发现仔猪的血清碱性磷酸酶随日龄的增加而呈明显的下降趋势，同时也验证了仔猪T淋巴细胞百分率随仔猪日龄的增加呈上升趋势，从而为仔猪免疫系统发育的研究提供重要的试验数据。
Three parts were included in this study:
1. Procedure of salting out in ammonium sulfate and procedure of caprylic acid depositting were used to extract IgG from porcine blood respectively in order to compare two methods and offer the foundation for making the better into practice. The results suggest that IgG extracted by procedure of caprylic acid deposition and purified by improved technique is more practical and feasible for its high concentration and activity and low production cost.
2. Eighty one-day-old piglets from 10 litters were randomly divided into four groups fed with different doses (0mg, 300mg, 600mg and 900mg) of IgG extracted respectively. Each animal's average-day-gain(ADG), diarrhoea days, serum Alkline phosphatase (AKP) activity and total protein and IgG, and percentage of T-lymphocyte cells of blood were measured on day 5, 10, and 15 after feeding. The experiment aims at evaluating newborn piglets' ability to absorb exogenous IgG and estimate exogenous IgG's influence on piglets' performance and immune capacity. The results showed that the group fed with high dose IgG (900mg) are higher than the contrast group in ADG between day 0 and 15, both serum AKP activity and total protein on day 5 and 10, and serum IgG concentration on day 5 significantly(p<0.05). At the same time, the high dose IgG can decrease the number of diarrhoea piglets and the diarrhoea duration of piglets. It can be concluded that one-day-old piglets have high ability to absorb
exogenous IgG and feeding newborn piglets with 900mg IgG on the basis of nature lactation can improve their performance and boichemical and immune parameters of blood.
3. Eighty 21-day-old piglets from another 10 litters were evenly divided into four groups injected with different doses (Omg, 300mg, 600mg and 900mg) of IgG extracted respectively, and the same parameters as part 2 were measured. The results showed that the group injected with high dosage of IgG (900mg) are better than the contrast group in ADG between day 21 and 30 and serum total protein significantly (p<0.05). Meanwhile, the number and duration of diarrhoea piglets are less than that of the contrast. Conclusion can be made that 21-day-old piglets injected with 900mg IgG from porcine blood have high performance and immunity.
According to results of part 2 and 3, quantity of AKP in piglets' blood has a decline trend with days increasing. On the contrary, T-lymphocyte cells show an increasing trend, which provide an important experimental data for future study on immune system development of piglet.
引文
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2．敖长金，侯先志，于志红，等．猪乳中IgG和有关生长因子的动态变化．内蒙古农牧学院学报，1999，19(2)：36-40
3．单虎，陈伟华，陆天水，等．初乳对新生仔猪免疫功能影响的研究．畜牧兽医学报，1998，29(3)：255-259
4．方福德，周吕．现代医学试验技巧全书(上册)．北京：北京医科大学中国协和医科大学联合出版社，1995，P547
5．侯西庚，李健德，张守信，等．非特异性酸性酯酶染色法对幼猪T淋巴细胞正常值的测定．兽医科技杂志，1982，1：29-30
6．蒋成淦．酶联免疫测定法．北京：人民卫生出版社，1984，p94-95
7．蒋守群编译．免疫球蛋白源对新生仔猪存活率、生产性能、血液因子和免疫因子的影响．饲料工业，2000，21(5)：11-15
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