栉江珧繁殖生物学及人工苗种繁育技术研究
|
摘要
我国贝类养殖业历史悠久,是海水养殖业的支柱产业,对国民经济的发展具有重要的推动作用。牡蛎等贝类都已实现了产业化,但经济价值很高的栉江珧却由于人工育苗技术的制约,迟迟未能形成规模化养殖。本文在东山湾栉江珧繁殖生物学调查的基础上,进行了亲贝促熟、催产方法的研究,并结合胚胎、幼虫发育的观察开展了人工苗种繁育技术的研究。
每月观察东山湾栉江珧性腺的发育情况,并测定性腺指数。结果表明:其性腺发育大致可分为增殖期、生长期、成熟期、排放期和休止期。生殖周期为一年,繁殖期在5～10月,繁殖高峰期分别出现在6月上旬和9月中旬,成熟排放期为6月上旬～10月上旬,此间的生长、成熟和排放各期相互重叠。故每年的3～4月,可采用室内升温和饵料强化培育的方法对性腺发育程度低的亲贝进行促熟,然后开展人工苗种生产;也可在5月中旬～6月上旬和8～9月,采捕海区性腺发育程度较高的栉江珧,经短期的室内饵料强化培育后进行人工催产和苗种生产。
研究中首次设计并使用了贝类仿生态培育及催产孔架,不仅对亲贝的培育效果好,而且做为催产的辅助器具,可明显缩短亲贝的阴干时间并提高排放率。在室内进行了恒温促熟,以及不同饵料、培育水温对性腺发育的影响实验,结果表明:恒温24℃左右,投喂湛江等鞭金藻和混合藻的培育效果好,经1个月左右,亲贝性腺可发育成熟。使用不同方法催产,结果表明:解剖法不适合用于人工苗种生产;自然排放法获得的精、卵质量最佳,但亲贝排放率最低;阴干流水法、紫外线照射法和温度刺激法的操作中均使用了贝类仿生态培育及催产孔架阴干亲贝,获得了50﹪～80﹪的排放率。但考虑到紫外线照射对胚胎发育的影响,故使用阴干流水法和温度刺激法催产更合适。
使用0.5m3的锥形底塑料孵化桶和6m3、18m3和24m3的水泥池培育幼虫,预实验中幼虫生长、发育观察和幼虫培育结果表明:幼虫对暗光有趋向性,而强光会影响其正常游动和摄食;幼虫发育的不同时期,壳长和壳高会表现为交替性的快速生长,引起D形幼虫、壳顶幼虫和稚贝间明显的形态差异;壳薄而脆弱,尤其是稚贝颗粒状的新生壳,稍受挤压就会破裂;幼虫培育期会出现漂浮黏连和附着变态难等问题,漂浮黏连、强光照和敌害生物等都是幼虫成活率低的原因。人工苗种培育结果表明:使用大水体培育栉江珧幼虫,保持适宜的光照强度,培育海水经紫外线杀菌并曝气,降低幼虫密度,在不同时期适当混合投喂新鲜高浓度和已过滤的饵料,经常换水和吸底,及时分选幼苗,做好海水各指标的监测和日常管理工作,可有效地防止幼虫黏连。首次使用了涤纶垫做为附着基,结果表明:涤纶垫铺薄砂吊于水中的附苗率和变态成活率最高,但易受污染,稚贝成活率低;而且池底铺砂和托盘装砂吊于池中的稚贝成活率也仅为27.54﹪和34.37﹪,故如何克服涤纶垫易受污染的缺点,并提高幼虫变态成活率和稚贝成活率仍有待进一步的研究。
The shellfish breeding is a mainstay mariculture industry with a long history in china, which still possessed significant contribution to the development of national economy. Due to the immature artificial rearing technique, the breeding industrialization of Atrina pectinata Linnaeus has not formed as oyster. The study included fattening and induced spawning methods and key technology for artificial larvae cultivation based on observation of embryonic and larvae development after the reproductive biology investigation of Atrina pectinata Linnaeus, Dongshan Bay.
The monthly development of gonad, determination result of gonad index indicated that the gonad matures once a year and the development of gonad could be approximately divided into proliferating period, growing period, maturing period, spawning period, and resting period. The idophase is from May to October. The peak of idophase respectively are the first ten days of June and middle of September. The peak of spawning period are from the first ten days of June to October. Growing period, maturing period and spawning period appeared simultaneously during the peak of spawning period. Therefore, it is feasible for fattening parent shellfish of low maturity by rising temperature of seawater indoor and strengthening nutrition from March to April every year, preparing for induced spawning and artificial cultivation of larvae. Furthermore, artificial breeding could be carried out after fattening parent shellfish of relatively higher maturity by strengthening nutrition for short period from middle of May to the first ten days of June, or from August to September.
It could obviously shorten dry exposure time and increase spawning rate of parent shellfish by using the multigrid shelf of simulated ecological culturing and induced spawning for shellfish as a assistive devices of inducing spawn, which was devised and applied for the first time. The gonad development results of parent shellfish reared under constant temperature, different microalgae and water temperature showed that gonad matured reared with feeding of Isochrysis zhanjiangensis and mixed algae under 24℃approximatly for 1 month approximately. Among the five methods of inducing spawning, dissection was not suitable for artificial breeding. Using method of natural discharge could acquired spermatozoa and ova of best quality but lowest release rate. Back to water after dry exposure, ultraviolet irradiation and variable temperature irritation obtained the highest release rate of 50-80﹪. However, ultraviolet irradiation was not applicable to induced spawning considering the harmful effects to development of embryonic and larvae.
The growth and development of larvae in the preliminary experiment showed that the larvae trend to move toward light sources under subdued light conditions. As the shell length and height grow rapidly by turns in the different developmental periods, it exhibits apparent difference among d-larvae, umbo larvae and juvenile shellfish. Shell, especially the neonatal shell of juvenile shellfish would break even if under slightly press. It frequently occur problem of larvae conglutination and difficult of metamorphosis etc in artificial larvae culture. All as problem of larvae conglutination, highlight, and harmful organisms etc are the reason of low survival. The result of artificial cultivation of larvae indicated that cement pool of large surface, suitable intensity of illumination, seawater of ultraviolet sterilization-treated and high oxygen dissolution, larvae of low density, filtered mixed algae of fresh and high concentration, changing the water and cleaning the bottom often, separate larvae of different size in time, monitoring physical and chemical index of seawater, and reinforcing routine management could effectively solve the problem of larvae conglutination. Pulvinus of dacron was first ever used as cultch in spatfall study, the result indicated that the method of paving thin sand on the Pulvinus of dacronthe obtained the highest adhesive rate and survival of metamorphosis period of young shellfish. Nevertheless the Pulvinus of dacronthe is easily polluted, and resulting in low survival of young shellfish. Furthermore, the young shellfish survival of other two methods are merely 27.54﹪and 34.37﹪. Consequently, there still need to study further how to overcome the disadvantage of spatfall using Pulvinus of dacronthe and raise the survival of metamorphosis period of young shellfish.
每月观察东山湾栉江珧性腺的发育情况,并测定性腺指数。结果表明:其性腺发育大致可分为增殖期、生长期、成熟期、排放期和休止期。生殖周期为一年,繁殖期在5～10月,繁殖高峰期分别出现在6月上旬和9月中旬,成熟排放期为6月上旬～10月上旬,此间的生长、成熟和排放各期相互重叠。故每年的3～4月,可采用室内升温和饵料强化培育的方法对性腺发育程度低的亲贝进行促熟,然后开展人工苗种生产;也可在5月中旬～6月上旬和8～9月,采捕海区性腺发育程度较高的栉江珧,经短期的室内饵料强化培育后进行人工催产和苗种生产。
研究中首次设计并使用了贝类仿生态培育及催产孔架,不仅对亲贝的培育效果好,而且做为催产的辅助器具,可明显缩短亲贝的阴干时间并提高排放率。在室内进行了恒温促熟,以及不同饵料、培育水温对性腺发育的影响实验,结果表明:恒温24℃左右,投喂湛江等鞭金藻和混合藻的培育效果好,经1个月左右,亲贝性腺可发育成熟。使用不同方法催产,结果表明:解剖法不适合用于人工苗种生产;自然排放法获得的精、卵质量最佳,但亲贝排放率最低;阴干流水法、紫外线照射法和温度刺激法的操作中均使用了贝类仿生态培育及催产孔架阴干亲贝,获得了50﹪～80﹪的排放率。但考虑到紫外线照射对胚胎发育的影响,故使用阴干流水法和温度刺激法催产更合适。
使用0.5m3的锥形底塑料孵化桶和6m3、18m3和24m3的水泥池培育幼虫,预实验中幼虫生长、发育观察和幼虫培育结果表明:幼虫对暗光有趋向性,而强光会影响其正常游动和摄食;幼虫发育的不同时期,壳长和壳高会表现为交替性的快速生长,引起D形幼虫、壳顶幼虫和稚贝间明显的形态差异;壳薄而脆弱,尤其是稚贝颗粒状的新生壳,稍受挤压就会破裂;幼虫培育期会出现漂浮黏连和附着变态难等问题,漂浮黏连、强光照和敌害生物等都是幼虫成活率低的原因。人工苗种培育结果表明:使用大水体培育栉江珧幼虫,保持适宜的光照强度,培育海水经紫外线杀菌并曝气,降低幼虫密度,在不同时期适当混合投喂新鲜高浓度和已过滤的饵料,经常换水和吸底,及时分选幼苗,做好海水各指标的监测和日常管理工作,可有效地防止幼虫黏连。首次使用了涤纶垫做为附着基,结果表明:涤纶垫铺薄砂吊于水中的附苗率和变态成活率最高,但易受污染,稚贝成活率低;而且池底铺砂和托盘装砂吊于池中的稚贝成活率也仅为27.54﹪和34.37﹪,故如何克服涤纶垫易受污染的缺点,并提高幼虫变态成活率和稚贝成活率仍有待进一步的研究。
The shellfish breeding is a mainstay mariculture industry with a long history in china, which still possessed significant contribution to the development of national economy. Due to the immature artificial rearing technique, the breeding industrialization of Atrina pectinata Linnaeus has not formed as oyster. The study included fattening and induced spawning methods and key technology for artificial larvae cultivation based on observation of embryonic and larvae development after the reproductive biology investigation of Atrina pectinata Linnaeus, Dongshan Bay.
The monthly development of gonad, determination result of gonad index indicated that the gonad matures once a year and the development of gonad could be approximately divided into proliferating period, growing period, maturing period, spawning period, and resting period. The idophase is from May to October. The peak of idophase respectively are the first ten days of June and middle of September. The peak of spawning period are from the first ten days of June to October. Growing period, maturing period and spawning period appeared simultaneously during the peak of spawning period. Therefore, it is feasible for fattening parent shellfish of low maturity by rising temperature of seawater indoor and strengthening nutrition from March to April every year, preparing for induced spawning and artificial cultivation of larvae. Furthermore, artificial breeding could be carried out after fattening parent shellfish of relatively higher maturity by strengthening nutrition for short period from middle of May to the first ten days of June, or from August to September.
It could obviously shorten dry exposure time and increase spawning rate of parent shellfish by using the multigrid shelf of simulated ecological culturing and induced spawning for shellfish as a assistive devices of inducing spawn, which was devised and applied for the first time. The gonad development results of parent shellfish reared under constant temperature, different microalgae and water temperature showed that gonad matured reared with feeding of Isochrysis zhanjiangensis and mixed algae under 24℃approximatly for 1 month approximately. Among the five methods of inducing spawning, dissection was not suitable for artificial breeding. Using method of natural discharge could acquired spermatozoa and ova of best quality but lowest release rate. Back to water after dry exposure, ultraviolet irradiation and variable temperature irritation obtained the highest release rate of 50-80﹪. However, ultraviolet irradiation was not applicable to induced spawning considering the harmful effects to development of embryonic and larvae.
The growth and development of larvae in the preliminary experiment showed that the larvae trend to move toward light sources under subdued light conditions. As the shell length and height grow rapidly by turns in the different developmental periods, it exhibits apparent difference among d-larvae, umbo larvae and juvenile shellfish. Shell, especially the neonatal shell of juvenile shellfish would break even if under slightly press. It frequently occur problem of larvae conglutination and difficult of metamorphosis etc in artificial larvae culture. All as problem of larvae conglutination, highlight, and harmful organisms etc are the reason of low survival. The result of artificial cultivation of larvae indicated that cement pool of large surface, suitable intensity of illumination, seawater of ultraviolet sterilization-treated and high oxygen dissolution, larvae of low density, filtered mixed algae of fresh and high concentration, changing the water and cleaning the bottom often, separate larvae of different size in time, monitoring physical and chemical index of seawater, and reinforcing routine management could effectively solve the problem of larvae conglutination. Pulvinus of dacron was first ever used as cultch in spatfall study, the result indicated that the method of paving thin sand on the Pulvinus of dacronthe obtained the highest adhesive rate and survival of metamorphosis period of young shellfish. Nevertheless the Pulvinus of dacronthe is easily polluted, and resulting in low survival of young shellfish. Furthermore, the young shellfish survival of other two methods are merely 27.54﹪and 34.37﹪. Consequently, there still need to study further how to overcome the disadvantage of spatfall using Pulvinus of dacronthe and raise the survival of metamorphosis period of young shellfish.
引文
[1]陈华絮.雷州市沿海几种贝类营养成分的分析[J].中国食物与营养, 2006 , (7): 49-51.
[2]雷晓凌,吴晓萍.南海八种贝类营养成分和限量元素含量的研究[J].中国海洋药物, 2001, 20 (2): 48-50.
[3]李丽,陶平,安凤飞.大连沿海8种双壳类贝的营养成分分析[J].中国公共卫生管理, 2003, 19 (2): 153-155.
[4]高翔,高健.大连主要海水贝类养殖状况分析[J].海洋开发与管理, 2006, 23 (6): 158-161.
[5]王如才,郑小东.我国海产贝类养殖进展及发展前景[J].中国海洋大学学报, 2004, 34 (5): 775-780.
[6]谢忠明.海水经济贝类养殖技术[M].北京:中国农业出版社, 2003: 1-9.
[7]王如才,王昭萍,张建中.海水贝类养殖学[M].山东:青岛海洋大学出版社, 1993: 1-4.
[8]黄璘.我国渔业科技进步贡献率已超过50%[J].中国科技投资, 2009, (3): 12.
[9]史岩,张小栓,穆维松,等.渔业产量增长与结构变化研究[J].莱阳农学院学报, 2004, 21 (1): 71-73.
[10]洪惠馨,林利民.我国渔业问题与对策[J].集美大学学报:自然科学版, 2002, 7 (1): 5-10.
[11]黄璘. 2006年全国渔业生产概况(上)[J].中国畜牧杂志, 2007, 43 (12): 12-14.
[12]沈建,林蔚,郁蔚文,等.我国贝类加工现状与发展前景[J].中国水产, 2008, (3): 73-75.
[13]陈舒宁.保障水产品质量安全疾病防控是关键——中国水产科学研究院珠江水产研究所所长吴淑勤研究员专访[J].广东饲料, 2008, (5): 9-12.
[14]小远.二OO七年全年渔业生产情况预测及影响因素[J].渔业致富指南, 2008, (2): 4-6.
[15] Lauenstein G, Cantillo A, Oconnor T. The status and trends of trace element and organic contaminants in oysters, Crassostrea virginica, in the waters of the Carolinas, USA[J]. Science of the Total Environment, 2002, 285 (1-3): 79-87.
[16]钱忠直,齐平,王国荣.中国药典[J].中国药品标准, 2005, 6 (1): 25-30.
[17] Mullendore J, Sobsey M, Carol Shieh Y. Improved method for the recovery of hepatitis A virus from oysters[J]. Journal of virological methods, 2001, 94 (1): 25-35.
[18] García-Rico L, Ruiz R, Jiménez J. Determination of total metals in cultivated oysters (crassostrea gigas) from the northwest coast of mexico by microwave digestion and atomic absorption spectrometry[J]. Journal of AOAC International, 2001, 84 (6): 1909-1913.
[19]叶盛权.牡蛎产品的开发研究[J].食品研究与开发, 2003, 24 (6): 83-85.
[20]刘政.海之奶牡蛎[J].饮食科学, 2004, (2): 21.
[21] Wang J, Hu J, Cui J, et al. Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the antihypertensive effect of hydrolysate in spontaneously hypertensive rats[J]. Food Chemistry, 2008, 111 (2): 302-308.
[22]付猛,云霞,朱蓓薇.牡蛎双酶水解工艺的研究[J].食品科学, 2004, 25(7): 100-104.
[23]谭雯文,周延华,梁海秋,等.牡蛎蛋白的纯化及酶解条件的研究[J].现代食品科技, 2006, 22 (2): 79-82.
[24]张水华.食品分析[M].北京:中国轻工业出版社, 2004: 79-82.
[25] Cruz-Romero M, Kelly A, Kerry J. Effects of high-pressure and heat treatments on physical and biochemical characteristics of oysters (Crassostrea gigas)[J]. Innovative Food Science and Emerging Technologies, 2007, 8 (1): 30-38.
[26]陈惠源,蔡俊鹏.牡蛎的营养药用价值及其开发利用[J].中药材, 2005, 28 (3): 172-174.
[27]滕瑜,王彩理.牡蛎的营养和降糖作用研究[J].海洋水产研究, 2005, 26 (6): 39-44.
[28]徐静.牡蛎提取物的降血糖活性研究[D].山东:山东大学, 2005.
[29] TANAKA K, NISHIZONO S, KUGINO K, et al. Effects of dietary oyster extract on lipid metabolism, blood pressure, and blood glucose in SD rats, hypertensive rats, and diabetic rats[J]. Bioscience, biotechnology, and biochemistry, 2006, 70 (2): 462-470.
[30]张辉.牡蛎活性肽降血糖和抑制ACE作用研究[D].广西:广西医科大学, 2009.
[31]刘赛,仲伟珍,张健,等.牡蛎提取物对鹌鹑实验性动脉粥样硬化的抑制作用及机制[J].中国动脉硬化杂志, 2002, 10 (2): 97-100.
[32] Kimura Y, Ohminami H, Okuda H. Effects of extract of oyster on lipid metabolism in rats[J]. Journal of ethnopharmacology, 1998, 59 (3): 117-123.
[33]于娅,杨瑞金,王璋.牡蛎功能短肽的制备及ACE抑制活性[J].无锡轻工大学学报:食品与生物技术, 2004, 23 (2): 49-52.
[34]欧成坤,杨瑞金.牡蛎酶解产物ACE抑制活性和清除自由基活性研究[J].食品工业科技, 2005, 26 (3): 59-62.
[35] Bordenave S, Arnaudin I, Sannier F, et al. Crassostrea gigas peptic hydrolysate: a source of various biologically active molecules[J]. Marine bio-technology:an overview of leading fields. 9th ESMB meeting, Nantes, 12, 2002, 36: 163-168.
[36] Achour A, Lachgar A, Astgen A, et al. Potentialization of IL-2 effects on immune cells by oyster extract (JCOE) in normal and HIV-infected individuals[J]. Biomedicine & Pharmacotherapy, 1997, 51 (10): 427-429.
[37]张冬青,黄山平.牡蛎提取物对小鼠免疫功能的正向调节作用[J].上海免疫学杂志, 1995, 15 (1): 18-21.
[38]王俊,姚滢,张建鹏,等.牡蛎多糖的制备和生物学活性研究[J].医学研究生学报, 2006, 19 (3): 217-220.
[39]王元勋,刘兆乾.扇贝、牡蛎提取液的抗疲劳作用的实验研究[J].体育科学, 1993, 13(6): 70-71.
[40]张部昌,商桂春.牡蛎口服液抗疲劳作用的研究[J].安徽大学学报:自然科学版, 1999, 23 (1): 103-106.
[41]王海桃.牡蛎糖胺聚糖保护血管内皮细胞作用的研究[D].山东:青岛大学, 2007.
[42]王海桃,刘赛.牡蛎糖胺聚糖对损伤的血管内皮细胞功能的影响[J].海洋水产研究, 2007, 28 (5): 105-109.
[43]吴海涛,张彧,缪琪,等.牡蛎水提液的抗氧化特性[J].食品与发酵工业, 2005, 31 (4): 42-45.
[44]陈曾三. JCOE (牡蛎提取物)的抗癌功能[J].杭州食品科技, 1997, (3): 11-14.
[45]王颖,赵振军.牡蛎提取物抗肿瘤作用的实验研究[J].中国海洋药物, 1997, 16 (1): 18-22.
[46]朱海波.牡蛎提取物对化学性肝损伤保护作用的研究[D].山东:烟台大学, 2007.
[47]李文立,赵敏,陈瑞仪,等.牡蛎粉对化学性肝损伤保护作用的实验研究[J].现代临床医学生物工程学杂志, 2003, 9 (6): 466-467.
[48]李旭,苑隆国,王晓辉.牡蛎提取物对小鼠肝脏保护作用研究[J].医学研究通讯, 2005, 34 (1): 51-52.
[49]王广军.鲍的养殖技术[J].水产科技, 2001, (6): 9-12.
[50]唐保军,王慧.我国鲍养殖研究现状及展望[J].渔业现代化, 2009, (1): 30-34.
[51]李钧,林祥志,谢双如,等.贝类养殖技术之一皱纹盘鲍南北接力养殖技术研究[J].中国水产, 2007, (11): 46-47.
[52]王吉桥,杨义,程鑫,等.不同密度的皱纹盘鲍(Haliotis discus hannai Ino)与仿刺参(Apostichopus japonicus Selenka)混养的研究[J].现代渔业信息, 2007, 22 (10): 3-7.
[53]常忠岳,胡志伟.潮间带养鲍池垒石养参技术[J].水产科学, 2000, 19 (1): 1.
[54]李胜宽,王立超,高永刚.潮间带围塘网箱鲍、海参混养技术[J].中国水产, 2001, 12: 63-64.
[55]刘刚,丁增明,孙先凯.海州湾浅海筏式参鲍混养技术[J].齐鲁渔业, 2009, 26(10): 38-39.
[56]李玉武,苏秀榕.皱纹盘鲍中几种营养成分的测定[J].中国海洋药物, 1995, 14 (1): 47-48.
[57]彭汶铎,陈启亮,赵金华.鲍鱼酶解提取物的营养成分及对免疫低下小鼠的免疫调节作用[J].中国食品卫生杂志, 2005, 17 (6): 494-497.
[58]许东晖,何泉华.皱纹盘鲍抗肿瘤活性物质的分离及药理研究[J].中药材, 1997, 20 (1): 40-41.
[59]王兵,蒋建敏.鲍鱼多糖对人鼻咽癌裸鼠抗癌作用的研究[J].中草药, 2000, 31 (8): 597-599.
[60]陈倩超.鲍鱼多糖的提取及抗肿瘤试验[J].中国现代应用药学, 1998, 15 (1): 8-10.
[61]魏利平.山东省扇贝养殖现状及持续发展的技术措施[J].齐鲁渔业, 2000, 17 (2): 21-23.
[62]段德麟,王远红.四种扇贝氨基酸和脂肪酸含量分析[J].营养学报, 1994, 16 (4): 433-435.
[63]杨凤影,张弼.不同地理种群栉孔扇贝营养成分的比较分析[J].安徽农业科学, 2009, 37 (9): 4073-4075.
[64]苏秀榕,李太武,丁明进.扇贝营养成分的研究[J].海洋科学, 1997, 2: 10-11.
[65]林文业,吴四海.扇贝肉营养成分分析研究[J].广东微量元素科学, 2000, 7 (1): 57-59.
[66]黄翠丽,刘赛.扇贝的生理活性物质和药用价值[J].中国海洋药物, 2001, 20 (2): 45-47.
[67] Yanagita T, Enomoto N, Yamamoto K. Cholesterol-lowering effect of Agemaki, a kind of shellfish, in mice[J]. JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 1991, 37: 313–318.
[68]侯文璞,徐明起,张守海,等.贻贝的营养价值[J].食品科学, 1985, 12 (1): 13-13.
[69]谢宗墉.海洋水产品营养与保健[M].青岛:青岛海洋大学出版社, 1991: 24-57.
[70]温扬敏,高如承.泥蚶的营养与药用价值[J].经济动物学报, 2009, 13 (3): 168-170.
[71]孙同秋,韩松,鞠东,等.渤海南部毛蚶营养成分分析及评价[J].齐鲁渔业, 2009, (8): 10-12.
[72]姜凤吾,张玉顺.中国海洋药物辞典[M].北京:海洋出版社, 1994: 67-68.
[73]冯维希,黄宜鹤.七种海产中药中微量元素含量测定[J].中药材, 1989, 12 (11): 35-36.
[74]张永普,贾守菊.不同种群泥蚶肉营养成分的比较研究[J].海洋湖沼通报, 2002, (2): 33-38.
[75]吴洪喜,柴雪良,李元中.乐清湾泥蚶肉营养成分的分析及评价[J].海洋科学, 2004, 28 (8): 19-22.
[76]李谦,李泰明.毛蚶提取物生化性质初步分析[J].药物生物技术, 1998, 5 (4): 245-247.
[77]燕春义,申洪波,王哲.毛蚶提取物对荷瘤小鼠免疫功能的影响[J].辽宁中医杂志, 2009, 36 (4): 656-657.
[78]王莉,何赟绵,姚全胜.毛蚶多糖免疫调节作用的实验研究[J].华西药学杂志, 2009, 24 (4): 340-342.
[79]胡雪琼,吴红棉,林志明,等.毛蚶糖胺聚糖的理化性质及其对小鼠免疫脏器影响的初步研究[J].现代食品科技, 2008, 24 (8): 763-766.
[80]窦昌贵,严永清,张征.毛蚶水解液降血糖降血脂的实验研究[J].中国海洋药物,1996, 15 (1) : 13.
[81]王贞丽,初晓,杨秀,等.泥蚶提取物抗肿瘤作用的实验研究[J].山东医药, 2006, 46 (3): 15-17.
[82]姚如永,初晓,张玉江,等.泥蚶提取物-海生素抗肿瘤作用的实验研究[J].中国海洋药物, 2005, 24 (01): 33-36.
[83]姚如永,初晓,陈守国,等.海洋泥蚶多肽抗肿瘤作用的实验研究[J].中国药学杂志, 2006, 41 (11): 868-870.
[84] Winckworth R. Marine Mollusca from South India and Ceylon. III: Pinna. With an Index to the Recent Species of Pinna[J]. Proceedings of the Malacological Society, 1929, 18: 176-297.
[85]梁象秋,方纪祖,杨和荃.水生生物学[M].北京:中国农业出版社, 1995: 200-252.
[86]吴天明,许章程.兴化湾栉江珧生态调查[J].海洋渔业, 1985, 7 (3): 106-110.
[87] Abbott R, Dance S, Abbott T. Compendium of seashells[M]. American Malacologists, Inc. Florida, 1988: 247-262.
[88] Bernard F, Cai Y, Morton B. Catalogue of the living marine bivalve molluscs of China[M]. Hong Kong: Hong Kong University Press, 1993: 1-121.
[89]张玺,齐钟彦,李洁民.南海双壳类软体动物[M].北京:科学出版社, 1960: 65-66.
[90]王祯瑞.中国近海江珧科的初步报告[C]//中国科学院海洋研究所.海洋科学集刊(5).山东:科学出版社, 1964, 5: 30-39.
[91]王祯瑞.中国动物志(软体动物门·双壳纲·贻贝目) [M].北京:科学出版社, 1997: 214-239.
[92]谢开恩,陈炳能,陈世杰,等.泉州湾栉江珧调查研究报告[J].福建水产科技, 1980, 2: 10-23.
[93]王如才,俞开康,姚善成.海水养殖技术手册[M].上海:上海科学技术出版社, 2001: 35-43.
[94]江宇,陈炳能.福建沿海栉江珧的生态习性观察[J].福建水产, 1981, 1: 27-29.
[95]董存有.栉江珧生态学的初步观察[J].四川动物, 1993, 12 (4): 29-31.
[96]任建峰,杨爱国.栉江珧研究现状及开发利用前景[J].海洋水产研究, 2005, 26 (4): 84-88.
[97]邱盛尧,杨建敏.栉江珧的繁殖生物学[J].水产学报, 2000, 24 (1): 28-31.
[98]王梅芳,余祥勇.北部湾及附近海域栉江珧性腺发育研究[J].广西科学, 2000, 7 (2): 140-143.
[99]王梅芳.江珧科经济种类同工酶表型差异与繁殖生物学研究[D].山东:青岛海洋大学, 2002.
[100]郭世茂,陈成枞,何丽璇,等.栉江珧人工育苗初步研究[J].海洋科学, 1987, 1: 35-39.
[101]林笔水,何进金,韦信敏,等.栉江珧人工育苗的初步研究[J].台湾海峡, 1987, 6 (3): 261-268.
[102]吕振波,丛日祥,曲世科,等.栉江珧人工育苗的初步研究[J].齐鲁渔业, 2004, 21 (6): 36-38.
[103]柯继.栉江珧人工育苗技术通过鉴定[J].水产科技, 1996, (1): 23-23.
[104]周维武,于瑞海.栉江珧人工升温繁育技术要点[J].渔业现代化, 2006, (1): 30-31.
[105]王海涛,王世党,周维武,等.栉江珧室内人工育苗技术[J].水产养殖, 2006, 27 (2): 34-36.
[106]曾祥玲,庞田胜,陈小宏.栉江珧人工育苗试验[J].海洋科学, 2005, 29 (8): 53-55.
[107]于瑞海,王昭萍,李琪,等.栉江珧工厂化育苗技术研究[J].中国海洋大学学报,2007, 37(5): 704-708.
[108] Inoue S, Oshima Y, Abe S, et al. Effects of tributyltin on the energy metabolism of pen shell (Atrina pectinata japonica)[J]. Chemosphere, 2007, 66 (7): 1226-1229.
[109]王梅芳,叶富良. 3种江珧同工酶遗传标记[J].湛江海洋大学学报, 2000, 20 (2): 1-5.
[110]余祥勇,王梅芳,杨书婷,等.栉江珧同工酶多态性的分析[J].华中农业大学学报, 2005, 24 (2): 203-206.
[111]杨青,李琪,于瑞海,等.人工诱导栉江珧雌核发育的初步研究[J].中国水产科学, 2006, 13 (2): 310-315.
[112]毛勇,余祥勇,周莉,等.应用RAPD技术初步分析无裂栉江珧四种类型的基因组DNA[J].广西科学, 2003, 10 (4): 309-311.
[113]邱盛尧,张锡佳.山东北部近海栉江珧的年龄与生长[J].水产学报, 1996, 20 (4): 301-306.
[114]吴宝铃.贝类繁殖附着变态生物学[M].济南:山东科学技术出版社, 1999: 14-27.
[115]罗伟,林聘玲,金启增.大亚湾环境与资源[M].合肥:安徽科学技术出版社, 1989: 352-358.
[116]陈冲,陈永亮.文蛤种贝人工促熟试验[J].水产科学, 1998, 17 (6): 9-11.
[117]于瑞海,孙志纲.魁蚶大水体升温育苗技术研究[J].海洋科学, 1995, (2): 5-7.
[118]赵新生,徐国成,万东善.毛蚶升温促熟人工育苗技术研究[J].中国水产, 2008, (3): 58-61.
[119]杜美荣,方建光,毛玉泽,等.栉孔扇贝春季升温育苗与保苗技术研究[J].南方水产, 2009, 5 (2): 40-44.
[120]张付国,郭建军,侯明泉.太平洋牡蛎亲贝升温促熟与产卵时间[J].中国水产, 1996(10): 31-32.
[121]罗有声.贻贝养殖技术[M].上海:上海科学技术出版社, 1983: 145-194.
[122]严正凛.九孔鲍的极体释放和第一次卵裂与水温的关系[J].上海海洋大学学报, 2009, 18 (2): 248-251.
[123]郑志芳,李雪艳,刘飞宏,等.利用臭氧刺激鲍产卵的试验研究与应用[J].齐鲁渔业, 2007, 24 (2): 31-32.
[124]王伟定,于谨兰,姚海富,等.皱纹盘鲍大规格苗种培育工艺流程和技术要点[J].上海水产大学学报, 2001, 10 (2): 179-182.
[125]严正凛,关瑞章,胡家财,等.紫外线照射剂量对九孔鲍胚胎及幼虫的影响[J].台湾海峡, 2007, 26 (3): 404-409.
[126]蔡英亚,张英,魏若飞.贝类学概论[M].上海:上海科学技术出版社, 1995: 28-30.
[127]孙晓庆,董树刚.生物饵料在水产养殖中的综合应用现状[J].齐鲁渔业, 2006, 23 (10): 31-33.
[128]张秋会,马莺.海洋微藻粗脂肪, EPA和DHA含量的测定[J].粮油食品科技, 2006, 14 (2): 48-49.
[129]蒋霞敏,郑亦周. 14种微藻总脂含量和脂肪酸组成研究[J].水生生物学报, 2003, 27 (3): 243-247.
[130]王明清,迟晓元,秦松,等.海洋微藻总脂含量和脂肪酸组成的测定[J].中国油脂, 2008, 33(11): 67-70.
[131]刘广丰,陈慧,沈和定,等.四种微藻对缢蛏稚贝暂养效果研究[J].水产科技情报, 2009, 36 (1): 44-47.
[132]刘永,张春芳,焦宗垚.大珠母贝对常用饵料摄食及消化能力研究[J].水产养殖, 2009, (10): 51-54.
[133]梁英,孙世春,魏建功,等.海水生物饵料培养技术[M].山东:青岛海洋大学出版社, 1998: 32-35.
[134]兰国宝,聂振平,赖彬.温度对泥蚶胚胎发育,幼体生长发育及变态的影响[J].广西科学, 1999, 6 (4): 307-310.
[2]雷晓凌,吴晓萍.南海八种贝类营养成分和限量元素含量的研究[J].中国海洋药物, 2001, 20 (2): 48-50.
[3]李丽,陶平,安凤飞.大连沿海8种双壳类贝的营养成分分析[J].中国公共卫生管理, 2003, 19 (2): 153-155.
[4]高翔,高健.大连主要海水贝类养殖状况分析[J].海洋开发与管理, 2006, 23 (6): 158-161.
[5]王如才,郑小东.我国海产贝类养殖进展及发展前景[J].中国海洋大学学报, 2004, 34 (5): 775-780.
[6]谢忠明.海水经济贝类养殖技术[M].北京:中国农业出版社, 2003: 1-9.
[7]王如才,王昭萍,张建中.海水贝类养殖学[M].山东:青岛海洋大学出版社, 1993: 1-4.
[8]黄璘.我国渔业科技进步贡献率已超过50%[J].中国科技投资, 2009, (3): 12.
[9]史岩,张小栓,穆维松,等.渔业产量增长与结构变化研究[J].莱阳农学院学报, 2004, 21 (1): 71-73.
[10]洪惠馨,林利民.我国渔业问题与对策[J].集美大学学报:自然科学版, 2002, 7 (1): 5-10.
[11]黄璘. 2006年全国渔业生产概况(上)[J].中国畜牧杂志, 2007, 43 (12): 12-14.
[12]沈建,林蔚,郁蔚文,等.我国贝类加工现状与发展前景[J].中国水产, 2008, (3): 73-75.
[13]陈舒宁.保障水产品质量安全疾病防控是关键——中国水产科学研究院珠江水产研究所所长吴淑勤研究员专访[J].广东饲料, 2008, (5): 9-12.
[14]小远.二OO七年全年渔业生产情况预测及影响因素[J].渔业致富指南, 2008, (2): 4-6.
[15] Lauenstein G, Cantillo A, Oconnor T. The status and trends of trace element and organic contaminants in oysters, Crassostrea virginica, in the waters of the Carolinas, USA[J]. Science of the Total Environment, 2002, 285 (1-3): 79-87.
[16]钱忠直,齐平,王国荣.中国药典[J].中国药品标准, 2005, 6 (1): 25-30.
[17] Mullendore J, Sobsey M, Carol Shieh Y. Improved method for the recovery of hepatitis A virus from oysters[J]. Journal of virological methods, 2001, 94 (1): 25-35.
[18] García-Rico L, Ruiz R, Jiménez J. Determination of total metals in cultivated oysters (crassostrea gigas) from the northwest coast of mexico by microwave digestion and atomic absorption spectrometry[J]. Journal of AOAC International, 2001, 84 (6): 1909-1913.
[19]叶盛权.牡蛎产品的开发研究[J].食品研究与开发, 2003, 24 (6): 83-85.
[20]刘政.海之奶牡蛎[J].饮食科学, 2004, (2): 21.
[21] Wang J, Hu J, Cui J, et al. Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the antihypertensive effect of hydrolysate in spontaneously hypertensive rats[J]. Food Chemistry, 2008, 111 (2): 302-308.
[22]付猛,云霞,朱蓓薇.牡蛎双酶水解工艺的研究[J].食品科学, 2004, 25(7): 100-104.
[23]谭雯文,周延华,梁海秋,等.牡蛎蛋白的纯化及酶解条件的研究[J].现代食品科技, 2006, 22 (2): 79-82.
[24]张水华.食品分析[M].北京:中国轻工业出版社, 2004: 79-82.
[25] Cruz-Romero M, Kelly A, Kerry J. Effects of high-pressure and heat treatments on physical and biochemical characteristics of oysters (Crassostrea gigas)[J]. Innovative Food Science and Emerging Technologies, 2007, 8 (1): 30-38.
[26]陈惠源,蔡俊鹏.牡蛎的营养药用价值及其开发利用[J].中药材, 2005, 28 (3): 172-174.
[27]滕瑜,王彩理.牡蛎的营养和降糖作用研究[J].海洋水产研究, 2005, 26 (6): 39-44.
[28]徐静.牡蛎提取物的降血糖活性研究[D].山东:山东大学, 2005.
[29] TANAKA K, NISHIZONO S, KUGINO K, et al. Effects of dietary oyster extract on lipid metabolism, blood pressure, and blood glucose in SD rats, hypertensive rats, and diabetic rats[J]. Bioscience, biotechnology, and biochemistry, 2006, 70 (2): 462-470.
[30]张辉.牡蛎活性肽降血糖和抑制ACE作用研究[D].广西:广西医科大学, 2009.
[31]刘赛,仲伟珍,张健,等.牡蛎提取物对鹌鹑实验性动脉粥样硬化的抑制作用及机制[J].中国动脉硬化杂志, 2002, 10 (2): 97-100.
[32] Kimura Y, Ohminami H, Okuda H. Effects of extract of oyster on lipid metabolism in rats[J]. Journal of ethnopharmacology, 1998, 59 (3): 117-123.
[33]于娅,杨瑞金,王璋.牡蛎功能短肽的制备及ACE抑制活性[J].无锡轻工大学学报:食品与生物技术, 2004, 23 (2): 49-52.
[34]欧成坤,杨瑞金.牡蛎酶解产物ACE抑制活性和清除自由基活性研究[J].食品工业科技, 2005, 26 (3): 59-62.
[35] Bordenave S, Arnaudin I, Sannier F, et al. Crassostrea gigas peptic hydrolysate: a source of various biologically active molecules[J]. Marine bio-technology:an overview of leading fields. 9th ESMB meeting, Nantes, 12, 2002, 36: 163-168.
[36] Achour A, Lachgar A, Astgen A, et al. Potentialization of IL-2 effects on immune cells by oyster extract (JCOE) in normal and HIV-infected individuals[J]. Biomedicine & Pharmacotherapy, 1997, 51 (10): 427-429.
[37]张冬青,黄山平.牡蛎提取物对小鼠免疫功能的正向调节作用[J].上海免疫学杂志, 1995, 15 (1): 18-21.
[38]王俊,姚滢,张建鹏,等.牡蛎多糖的制备和生物学活性研究[J].医学研究生学报, 2006, 19 (3): 217-220.
[39]王元勋,刘兆乾.扇贝、牡蛎提取液的抗疲劳作用的实验研究[J].体育科学, 1993, 13(6): 70-71.
[40]张部昌,商桂春.牡蛎口服液抗疲劳作用的研究[J].安徽大学学报:自然科学版, 1999, 23 (1): 103-106.
[41]王海桃.牡蛎糖胺聚糖保护血管内皮细胞作用的研究[D].山东:青岛大学, 2007.
[42]王海桃,刘赛.牡蛎糖胺聚糖对损伤的血管内皮细胞功能的影响[J].海洋水产研究, 2007, 28 (5): 105-109.
[43]吴海涛,张彧,缪琪,等.牡蛎水提液的抗氧化特性[J].食品与发酵工业, 2005, 31 (4): 42-45.
[44]陈曾三. JCOE (牡蛎提取物)的抗癌功能[J].杭州食品科技, 1997, (3): 11-14.
[45]王颖,赵振军.牡蛎提取物抗肿瘤作用的实验研究[J].中国海洋药物, 1997, 16 (1): 18-22.
[46]朱海波.牡蛎提取物对化学性肝损伤保护作用的研究[D].山东:烟台大学, 2007.
[47]李文立,赵敏,陈瑞仪,等.牡蛎粉对化学性肝损伤保护作用的实验研究[J].现代临床医学生物工程学杂志, 2003, 9 (6): 466-467.
[48]李旭,苑隆国,王晓辉.牡蛎提取物对小鼠肝脏保护作用研究[J].医学研究通讯, 2005, 34 (1): 51-52.
[49]王广军.鲍的养殖技术[J].水产科技, 2001, (6): 9-12.
[50]唐保军,王慧.我国鲍养殖研究现状及展望[J].渔业现代化, 2009, (1): 30-34.
[51]李钧,林祥志,谢双如,等.贝类养殖技术之一皱纹盘鲍南北接力养殖技术研究[J].中国水产, 2007, (11): 46-47.
[52]王吉桥,杨义,程鑫,等.不同密度的皱纹盘鲍(Haliotis discus hannai Ino)与仿刺参(Apostichopus japonicus Selenka)混养的研究[J].现代渔业信息, 2007, 22 (10): 3-7.
[53]常忠岳,胡志伟.潮间带养鲍池垒石养参技术[J].水产科学, 2000, 19 (1): 1.
[54]李胜宽,王立超,高永刚.潮间带围塘网箱鲍、海参混养技术[J].中国水产, 2001, 12: 63-64.
[55]刘刚,丁增明,孙先凯.海州湾浅海筏式参鲍混养技术[J].齐鲁渔业, 2009, 26(10): 38-39.
[56]李玉武,苏秀榕.皱纹盘鲍中几种营养成分的测定[J].中国海洋药物, 1995, 14 (1): 47-48.
[57]彭汶铎,陈启亮,赵金华.鲍鱼酶解提取物的营养成分及对免疫低下小鼠的免疫调节作用[J].中国食品卫生杂志, 2005, 17 (6): 494-497.
[58]许东晖,何泉华.皱纹盘鲍抗肿瘤活性物质的分离及药理研究[J].中药材, 1997, 20 (1): 40-41.
[59]王兵,蒋建敏.鲍鱼多糖对人鼻咽癌裸鼠抗癌作用的研究[J].中草药, 2000, 31 (8): 597-599.
[60]陈倩超.鲍鱼多糖的提取及抗肿瘤试验[J].中国现代应用药学, 1998, 15 (1): 8-10.
[61]魏利平.山东省扇贝养殖现状及持续发展的技术措施[J].齐鲁渔业, 2000, 17 (2): 21-23.
[62]段德麟,王远红.四种扇贝氨基酸和脂肪酸含量分析[J].营养学报, 1994, 16 (4): 433-435.
[63]杨凤影,张弼.不同地理种群栉孔扇贝营养成分的比较分析[J].安徽农业科学, 2009, 37 (9): 4073-4075.
[64]苏秀榕,李太武,丁明进.扇贝营养成分的研究[J].海洋科学, 1997, 2: 10-11.
[65]林文业,吴四海.扇贝肉营养成分分析研究[J].广东微量元素科学, 2000, 7 (1): 57-59.
[66]黄翠丽,刘赛.扇贝的生理活性物质和药用价值[J].中国海洋药物, 2001, 20 (2): 45-47.
[67] Yanagita T, Enomoto N, Yamamoto K. Cholesterol-lowering effect of Agemaki, a kind of shellfish, in mice[J]. JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 1991, 37: 313–318.
[68]侯文璞,徐明起,张守海,等.贻贝的营养价值[J].食品科学, 1985, 12 (1): 13-13.
[69]谢宗墉.海洋水产品营养与保健[M].青岛:青岛海洋大学出版社, 1991: 24-57.
[70]温扬敏,高如承.泥蚶的营养与药用价值[J].经济动物学报, 2009, 13 (3): 168-170.
[71]孙同秋,韩松,鞠东,等.渤海南部毛蚶营养成分分析及评价[J].齐鲁渔业, 2009, (8): 10-12.
[72]姜凤吾,张玉顺.中国海洋药物辞典[M].北京:海洋出版社, 1994: 67-68.
[73]冯维希,黄宜鹤.七种海产中药中微量元素含量测定[J].中药材, 1989, 12 (11): 35-36.
[74]张永普,贾守菊.不同种群泥蚶肉营养成分的比较研究[J].海洋湖沼通报, 2002, (2): 33-38.
[75]吴洪喜,柴雪良,李元中.乐清湾泥蚶肉营养成分的分析及评价[J].海洋科学, 2004, 28 (8): 19-22.
[76]李谦,李泰明.毛蚶提取物生化性质初步分析[J].药物生物技术, 1998, 5 (4): 245-247.
[77]燕春义,申洪波,王哲.毛蚶提取物对荷瘤小鼠免疫功能的影响[J].辽宁中医杂志, 2009, 36 (4): 656-657.
[78]王莉,何赟绵,姚全胜.毛蚶多糖免疫调节作用的实验研究[J].华西药学杂志, 2009, 24 (4): 340-342.
[79]胡雪琼,吴红棉,林志明,等.毛蚶糖胺聚糖的理化性质及其对小鼠免疫脏器影响的初步研究[J].现代食品科技, 2008, 24 (8): 763-766.
[80]窦昌贵,严永清,张征.毛蚶水解液降血糖降血脂的实验研究[J].中国海洋药物,1996, 15 (1) : 13.
[81]王贞丽,初晓,杨秀,等.泥蚶提取物抗肿瘤作用的实验研究[J].山东医药, 2006, 46 (3): 15-17.
[82]姚如永,初晓,张玉江,等.泥蚶提取物-海生素抗肿瘤作用的实验研究[J].中国海洋药物, 2005, 24 (01): 33-36.
[83]姚如永,初晓,陈守国,等.海洋泥蚶多肽抗肿瘤作用的实验研究[J].中国药学杂志, 2006, 41 (11): 868-870.
[84] Winckworth R. Marine Mollusca from South India and Ceylon. III: Pinna. With an Index to the Recent Species of Pinna[J]. Proceedings of the Malacological Society, 1929, 18: 176-297.
[85]梁象秋,方纪祖,杨和荃.水生生物学[M].北京:中国农业出版社, 1995: 200-252.
[86]吴天明,许章程.兴化湾栉江珧生态调查[J].海洋渔业, 1985, 7 (3): 106-110.
[87] Abbott R, Dance S, Abbott T. Compendium of seashells[M]. American Malacologists, Inc. Florida, 1988: 247-262.
[88] Bernard F, Cai Y, Morton B. Catalogue of the living marine bivalve molluscs of China[M]. Hong Kong: Hong Kong University Press, 1993: 1-121.
[89]张玺,齐钟彦,李洁民.南海双壳类软体动物[M].北京:科学出版社, 1960: 65-66.
[90]王祯瑞.中国近海江珧科的初步报告[C]//中国科学院海洋研究所.海洋科学集刊(5).山东:科学出版社, 1964, 5: 30-39.
[91]王祯瑞.中国动物志(软体动物门·双壳纲·贻贝目) [M].北京:科学出版社, 1997: 214-239.
[92]谢开恩,陈炳能,陈世杰,等.泉州湾栉江珧调查研究报告[J].福建水产科技, 1980, 2: 10-23.
[93]王如才,俞开康,姚善成.海水养殖技术手册[M].上海:上海科学技术出版社, 2001: 35-43.
[94]江宇,陈炳能.福建沿海栉江珧的生态习性观察[J].福建水产, 1981, 1: 27-29.
[95]董存有.栉江珧生态学的初步观察[J].四川动物, 1993, 12 (4): 29-31.
[96]任建峰,杨爱国.栉江珧研究现状及开发利用前景[J].海洋水产研究, 2005, 26 (4): 84-88.
[97]邱盛尧,杨建敏.栉江珧的繁殖生物学[J].水产学报, 2000, 24 (1): 28-31.
[98]王梅芳,余祥勇.北部湾及附近海域栉江珧性腺发育研究[J].广西科学, 2000, 7 (2): 140-143.
[99]王梅芳.江珧科经济种类同工酶表型差异与繁殖生物学研究[D].山东:青岛海洋大学, 2002.
[100]郭世茂,陈成枞,何丽璇,等.栉江珧人工育苗初步研究[J].海洋科学, 1987, 1: 35-39.
[101]林笔水,何进金,韦信敏,等.栉江珧人工育苗的初步研究[J].台湾海峡, 1987, 6 (3): 261-268.
[102]吕振波,丛日祥,曲世科,等.栉江珧人工育苗的初步研究[J].齐鲁渔业, 2004, 21 (6): 36-38.
[103]柯继.栉江珧人工育苗技术通过鉴定[J].水产科技, 1996, (1): 23-23.
[104]周维武,于瑞海.栉江珧人工升温繁育技术要点[J].渔业现代化, 2006, (1): 30-31.
[105]王海涛,王世党,周维武,等.栉江珧室内人工育苗技术[J].水产养殖, 2006, 27 (2): 34-36.
[106]曾祥玲,庞田胜,陈小宏.栉江珧人工育苗试验[J].海洋科学, 2005, 29 (8): 53-55.
[107]于瑞海,王昭萍,李琪,等.栉江珧工厂化育苗技术研究[J].中国海洋大学学报,2007, 37(5): 704-708.
[108] Inoue S, Oshima Y, Abe S, et al. Effects of tributyltin on the energy metabolism of pen shell (Atrina pectinata japonica)[J]. Chemosphere, 2007, 66 (7): 1226-1229.
[109]王梅芳,叶富良. 3种江珧同工酶遗传标记[J].湛江海洋大学学报, 2000, 20 (2): 1-5.
[110]余祥勇,王梅芳,杨书婷,等.栉江珧同工酶多态性的分析[J].华中农业大学学报, 2005, 24 (2): 203-206.
[111]杨青,李琪,于瑞海,等.人工诱导栉江珧雌核发育的初步研究[J].中国水产科学, 2006, 13 (2): 310-315.
[112]毛勇,余祥勇,周莉,等.应用RAPD技术初步分析无裂栉江珧四种类型的基因组DNA[J].广西科学, 2003, 10 (4): 309-311.
[113]邱盛尧,张锡佳.山东北部近海栉江珧的年龄与生长[J].水产学报, 1996, 20 (4): 301-306.
[114]吴宝铃.贝类繁殖附着变态生物学[M].济南:山东科学技术出版社, 1999: 14-27.
[115]罗伟,林聘玲,金启增.大亚湾环境与资源[M].合肥:安徽科学技术出版社, 1989: 352-358.
[116]陈冲,陈永亮.文蛤种贝人工促熟试验[J].水产科学, 1998, 17 (6): 9-11.
[117]于瑞海,孙志纲.魁蚶大水体升温育苗技术研究[J].海洋科学, 1995, (2): 5-7.
[118]赵新生,徐国成,万东善.毛蚶升温促熟人工育苗技术研究[J].中国水产, 2008, (3): 58-61.
[119]杜美荣,方建光,毛玉泽,等.栉孔扇贝春季升温育苗与保苗技术研究[J].南方水产, 2009, 5 (2): 40-44.
[120]张付国,郭建军,侯明泉.太平洋牡蛎亲贝升温促熟与产卵时间[J].中国水产, 1996(10): 31-32.
[121]罗有声.贻贝养殖技术[M].上海:上海科学技术出版社, 1983: 145-194.
[122]严正凛.九孔鲍的极体释放和第一次卵裂与水温的关系[J].上海海洋大学学报, 2009, 18 (2): 248-251.
[123]郑志芳,李雪艳,刘飞宏,等.利用臭氧刺激鲍产卵的试验研究与应用[J].齐鲁渔业, 2007, 24 (2): 31-32.
[124]王伟定,于谨兰,姚海富,等.皱纹盘鲍大规格苗种培育工艺流程和技术要点[J].上海水产大学学报, 2001, 10 (2): 179-182.
[125]严正凛,关瑞章,胡家财,等.紫外线照射剂量对九孔鲍胚胎及幼虫的影响[J].台湾海峡, 2007, 26 (3): 404-409.
[126]蔡英亚,张英,魏若飞.贝类学概论[M].上海:上海科学技术出版社, 1995: 28-30.
[127]孙晓庆,董树刚.生物饵料在水产养殖中的综合应用现状[J].齐鲁渔业, 2006, 23 (10): 31-33.
[128]张秋会,马莺.海洋微藻粗脂肪, EPA和DHA含量的测定[J].粮油食品科技, 2006, 14 (2): 48-49.
[129]蒋霞敏,郑亦周. 14种微藻总脂含量和脂肪酸组成研究[J].水生生物学报, 2003, 27 (3): 243-247.
[130]王明清,迟晓元,秦松,等.海洋微藻总脂含量和脂肪酸组成的测定[J].中国油脂, 2008, 33(11): 67-70.
[131]刘广丰,陈慧,沈和定,等.四种微藻对缢蛏稚贝暂养效果研究[J].水产科技情报, 2009, 36 (1): 44-47.
[132]刘永,张春芳,焦宗垚.大珠母贝对常用饵料摄食及消化能力研究[J].水产养殖, 2009, (10): 51-54.
[133]梁英,孙世春,魏建功,等.海水生物饵料培养技术[M].山东:青岛海洋大学出版社, 1998: 32-35.
[134]兰国宝,聂振平,赖彬.温度对泥蚶胚胎发育,幼体生长发育及变态的影响[J].广西科学, 1999, 6 (4): 307-310.