紫茎泽兰分子研究平台的建立及化感相关基因EaCHS1的克隆
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摘要
紫茎泽兰(Eupatorium adenophorum. Sprengel)是一种入侵性很强的恶性杂草,起源于墨西哥和哥斯达黎加一带,它具有生命力强、传播速度快,植株本身毒性大,对土壤养分的吸收性强,分泌化感物质等特点,目前已经广泛分布于世界热带、亚热带30多个国家和地区,对森林、农田等生态系统造成的严重威胁,对入侵地区的经济、生态和社会造成了重大损失。该恶性杂草于20世纪40年代由中缅边境传入我国,在国家环保局首批定为最重要的16种外来入侵物种中,紫茎泽兰名列首位。目前国内外对于紫茎泽兰的研究主要集中在其入侵模式、生态学特性、防除方法和利用等方面。紫茎泽兰化感物质在生物入侵过程中发挥了重要的作用,其作用机制可能是通过影响植物光合作用、各种代谢以及根系生长和对矿质元素吸收等许多方面来实现入侵。然而目前,对于与次生代谢相关的化感物质的代谢调控机理方面的研究还很少,本文就是围绕这一点开展研究工作。针对紫茎泽兰基因的克隆和功能分析建立了研究平台,通过开展紫茎泽兰的化感相关的代谢途径的研究,为这种恶性杂草的综合治理提供理论依据,以期能够找到调控化感物质代谢的关键酶的编码基因,发掘一些能够为农业生产所利用的新基因。本论文的研究结果如下:
1.构建了紫茎泽兰叶片cDNA文库。通过SMART的方法合作构建了高质量的紫茎泽兰cDNA文库。原始文库的滴度为1.23×107 cfu/mL,重组率达98 %,插入片段平均大小在900 bp左右,扩增文库滴度达到了1×1012,覆盖率达99 %以上,利用该文库筛选得到了紫茎泽兰核酮糖二磷酸羧化酶基因(RuBP)的cDNA片段(GenBank ID:FJ752421),表明文库质量合格。
2.建立了紫茎泽兰的遗传转化体系。通过农杆菌介导的方法建立了紫茎泽兰的遗传转化体系。经过多种检测手段的鉴定,目前已经获得7株转基因植株,转化效率达到2 %。
3.化感相关基因的克隆、筛选及表达谱研究。克隆了4个紫茎泽兰的基因片段,分别是3-羟基-3-甲基戊二酰辅酶A合成酶(HMGS)基因(GenBank ID:FJ913887)、1-去氧木糖-5-磷酸异构酶(DXR)基因(GenBank ID:FJ752422)、倍半萜烯环化酶(TS)基因(GenBank ID:FJ747651)和查尔酮合酶(CHS)基因。荧光定量PCR的结果表明,萜类代谢途径中的两个关键酶基因(HMGS和DXR)的表达水平,没有因为羟基泽兰酮的诱导而表现出显著的差异;CHS基因的表达水平,除了在羟基泽兰酮处理浓度为1.0 mM时的差异不显著之外,在其他处理浓度均有不同程度的下调,说明该基因与化感物质(羟基泽兰酮)的代谢途径相关。
4. EaCHS1基因全长的克隆及表达载体构建。通过RACE技术,首次克隆了紫茎泽兰查尔酮合酶基因的全长(GenBank ID:FJ913888),将其命名为EaCHS1。EaCHS1全长1178 bp,其中5’非翻译区27 bp,3’非翻译区200 bp,最大开放阅读框架948 bp,编码316个氨基酸,理论分子量为33.9 kDa。根据EaCHS1基因的3’非翻译区序列设计引物,进行Real-Time PCR检测,结果发现:经过不同浓度羟基泽兰酮处理,EaCHS1基因的表达水平出现不同程度上调,只在浓度为1.0 mM时的差异不显著。Southern杂交表明,EaCHS1基因在紫茎泽兰中不多于4个拷贝。针对EaCHS1基因构建了植物过量表达载体并利用紫茎泽兰CHS基因的保守序列构建了RNAi载体,分别转化紫茎泽兰和烟草;
5.羟基泽兰酮对植物根系发育的影响。利用模式植物—拟南芥,初步探索了化感物质(羟基泽兰酮)对受体植物根系发育的影响。结果表明:拟南芥根系成熟区的根毛生长在羟基泽兰酮浓度为0.025 mM时,受到促进,而在0.1 mM时开始受到抑制;主根的生长受羟基泽兰酮的影响,存在一个明显的低浓度促进、高浓度抑制的现象。
Eupatorium adenophorum is a kind of noxious invasive alien weed origin from Mexico and Costarica. It has some characteristics such as strong vitality and toxicity; it also can spread rapidly, absorb nutrients from soil strongly, and produce allelochemicals. At present, Eupatorium adenophorum has widely distributed about 30 countries and regions at tropical and subtropical zones. In these regions, it has threatened the ecological system of forest and farmland badly. As a result, it has caused serious loss in economy, ecology and society. This worst weed was firstly introduced into China from Burma in 1940s. In the 16 types of exotic invasive species confirmed by the Ministry of Environmental Protection of China, E. adenophorum is the first one as the most ecologically significant and potentially destructive species for China. Up to now, most of the study was focused on the invasion and dissemination patterns of E. adenophorum, its physiological ecology, prevention and utilization of the weed. Those allelochemicals of E. adenophorum play an important role in process of biological invasion. Its mechanism probably influences many aspects in plant, such as photosynthesis, various metabolisms, root growth and metal elements absorption. Little research was reported about regulation mechanism of allelochemical in the secondary metabolic pathways. Our research was carried out encircling this point.A research platform was constructed for gene cloning and functional analysis in Eupatorium adenophorum in this study to provide a theoretical basis for the all-sided control of this malignant weed through the research in metabolic pathway related to allelochemical. By the study, we try to find out the coding gene of key enzyme which is related with the metabolism of allelochemical and clone some novel genes which can be used in agricultural production. The results are as follows:
1. A cDNA library was constructed by using leaves of E. adenophorum as tissue materials and through the switching mechanism at 5’end of RNA transcript (SMART) method. The titer of primary library is 1.23×107 cfu/ml, and 98 % clones are recombinants. The average size of insert fragments is about 900 bp. The titer of amplified library is 1×1012, and 99 % coverage rates are obtained. The RuBP gene of E. adenophorum was screened out from this cDNA library (GenBank ID: FJ752421), this mean that the cDNA library has the qualified quality.
2. A effective protocol for the Agrobacterium tumefaciens-mediated genetic transformation of E. adenophorum with tender stem segments as tissue materials was constructed. Status of transgenic plants was confirmed by a variety of experiments including RT-PCR, PCR and GUS histochemical assays. The transformation efficiency in E. adenophorum was 2 %, and 7 transgenetic plants were obtained by now.
3. Four genes related to allelochemical were cloned, screened and analysed. They are 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGS) (GenBank ID: FJ913887), 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) (GenBank ID: FJ752422), sesquiterpene cyclase (TS) (GenBank ID: FJ747651) and Chalcone synthase (CHS). The real-time quantitative PCR results showed that the expression level of two genes (HMGS and DXR) did not be affected by HHO obviously, whereas, the expression level of CHS gene had down regulation of different degree, except in 1.0 mM of HHO’s concentration. It meaned that CHS gene is probably related with the metabolism of allelochemical (HHO).
4. The Chalcone synthase gene (EaCHS1) of E. adenophorum was cloned by RACE method (GenBank ID: FJ913888). The complete nucleotide sequence of EaCHS1 gene contains 1178 base pairs, deducing 316 amino acids. It was comprised of 5'-noncoding region (27 bp), open reading frame (948 bp) and 3'-noncoding region (200 bp). The molecular mass of the deduced protein is 33.9 kDa. Using the sequence of 3’UTR in EaCHS1 gene as primers, the real-time quantitative PCR result showed that the expression level of EaCHS1 gene was up regulated when induced by HHO except for in 1.0 mM of HHO. Southern blotting shows that EaCHS1 gene has four copies in E. adenophorum. We also constructed the over-expression vector of EaCHS1 gene and RNA interference vector which used the conservative sequence of CHS gene in E. adenophorum as target sequence respectively, and then transferred them into E. adenophorum and tobacco.
5. Arabidopsis Thaliana, as a kind of model plant, was used as receptor plant in order to preliminarily explore the root development affected by HHO. The result indicated that the growth of root hair was promoted in the root maturation zone of A. Thaliana when the concentration of HHO was 0.025 mM, whereas it was inhibited when the concentration of HHO was 0.1 mM and higher. This phenomenon showed that the growth of main root was promoted in a low concentration and inhibited in a high concentration of HHO.
1.构建了紫茎泽兰叶片cDNA文库。通过SMART的方法合作构建了高质量的紫茎泽兰cDNA文库。原始文库的滴度为1.23×107 cfu/mL,重组率达98 %,插入片段平均大小在900 bp左右,扩增文库滴度达到了1×1012,覆盖率达99 %以上,利用该文库筛选得到了紫茎泽兰核酮糖二磷酸羧化酶基因(RuBP)的cDNA片段(GenBank ID:FJ752421),表明文库质量合格。
2.建立了紫茎泽兰的遗传转化体系。通过农杆菌介导的方法建立了紫茎泽兰的遗传转化体系。经过多种检测手段的鉴定,目前已经获得7株转基因植株,转化效率达到2 %。
3.化感相关基因的克隆、筛选及表达谱研究。克隆了4个紫茎泽兰的基因片段,分别是3-羟基-3-甲基戊二酰辅酶A合成酶(HMGS)基因(GenBank ID:FJ913887)、1-去氧木糖-5-磷酸异构酶(DXR)基因(GenBank ID:FJ752422)、倍半萜烯环化酶(TS)基因(GenBank ID:FJ747651)和查尔酮合酶(CHS)基因。荧光定量PCR的结果表明,萜类代谢途径中的两个关键酶基因(HMGS和DXR)的表达水平,没有因为羟基泽兰酮的诱导而表现出显著的差异;CHS基因的表达水平,除了在羟基泽兰酮处理浓度为1.0 mM时的差异不显著之外,在其他处理浓度均有不同程度的下调,说明该基因与化感物质(羟基泽兰酮)的代谢途径相关。
4. EaCHS1基因全长的克隆及表达载体构建。通过RACE技术,首次克隆了紫茎泽兰查尔酮合酶基因的全长(GenBank ID:FJ913888),将其命名为EaCHS1。EaCHS1全长1178 bp,其中5’非翻译区27 bp,3’非翻译区200 bp,最大开放阅读框架948 bp,编码316个氨基酸,理论分子量为33.9 kDa。根据EaCHS1基因的3’非翻译区序列设计引物,进行Real-Time PCR检测,结果发现:经过不同浓度羟基泽兰酮处理,EaCHS1基因的表达水平出现不同程度上调,只在浓度为1.0 mM时的差异不显著。Southern杂交表明,EaCHS1基因在紫茎泽兰中不多于4个拷贝。针对EaCHS1基因构建了植物过量表达载体并利用紫茎泽兰CHS基因的保守序列构建了RNAi载体,分别转化紫茎泽兰和烟草;
5.羟基泽兰酮对植物根系发育的影响。利用模式植物—拟南芥,初步探索了化感物质(羟基泽兰酮)对受体植物根系发育的影响。结果表明:拟南芥根系成熟区的根毛生长在羟基泽兰酮浓度为0.025 mM时,受到促进,而在0.1 mM时开始受到抑制;主根的生长受羟基泽兰酮的影响,存在一个明显的低浓度促进、高浓度抑制的现象。
Eupatorium adenophorum is a kind of noxious invasive alien weed origin from Mexico and Costarica. It has some characteristics such as strong vitality and toxicity; it also can spread rapidly, absorb nutrients from soil strongly, and produce allelochemicals. At present, Eupatorium adenophorum has widely distributed about 30 countries and regions at tropical and subtropical zones. In these regions, it has threatened the ecological system of forest and farmland badly. As a result, it has caused serious loss in economy, ecology and society. This worst weed was firstly introduced into China from Burma in 1940s. In the 16 types of exotic invasive species confirmed by the Ministry of Environmental Protection of China, E. adenophorum is the first one as the most ecologically significant and potentially destructive species for China. Up to now, most of the study was focused on the invasion and dissemination patterns of E. adenophorum, its physiological ecology, prevention and utilization of the weed. Those allelochemicals of E. adenophorum play an important role in process of biological invasion. Its mechanism probably influences many aspects in plant, such as photosynthesis, various metabolisms, root growth and metal elements absorption. Little research was reported about regulation mechanism of allelochemical in the secondary metabolic pathways. Our research was carried out encircling this point.A research platform was constructed for gene cloning and functional analysis in Eupatorium adenophorum in this study to provide a theoretical basis for the all-sided control of this malignant weed through the research in metabolic pathway related to allelochemical. By the study, we try to find out the coding gene of key enzyme which is related with the metabolism of allelochemical and clone some novel genes which can be used in agricultural production. The results are as follows:
1. A cDNA library was constructed by using leaves of E. adenophorum as tissue materials and through the switching mechanism at 5’end of RNA transcript (SMART) method. The titer of primary library is 1.23×107 cfu/ml, and 98 % clones are recombinants. The average size of insert fragments is about 900 bp. The titer of amplified library is 1×1012, and 99 % coverage rates are obtained. The RuBP gene of E. adenophorum was screened out from this cDNA library (GenBank ID: FJ752421), this mean that the cDNA library has the qualified quality.
2. A effective protocol for the Agrobacterium tumefaciens-mediated genetic transformation of E. adenophorum with tender stem segments as tissue materials was constructed. Status of transgenic plants was confirmed by a variety of experiments including RT-PCR, PCR and GUS histochemical assays. The transformation efficiency in E. adenophorum was 2 %, and 7 transgenetic plants were obtained by now.
3. Four genes related to allelochemical were cloned, screened and analysed. They are 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGS) (GenBank ID: FJ913887), 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) (GenBank ID: FJ752422), sesquiterpene cyclase (TS) (GenBank ID: FJ747651) and Chalcone synthase (CHS). The real-time quantitative PCR results showed that the expression level of two genes (HMGS and DXR) did not be affected by HHO obviously, whereas, the expression level of CHS gene had down regulation of different degree, except in 1.0 mM of HHO’s concentration. It meaned that CHS gene is probably related with the metabolism of allelochemical (HHO).
4. The Chalcone synthase gene (EaCHS1) of E. adenophorum was cloned by RACE method (GenBank ID: FJ913888). The complete nucleotide sequence of EaCHS1 gene contains 1178 base pairs, deducing 316 amino acids. It was comprised of 5'-noncoding region (27 bp), open reading frame (948 bp) and 3'-noncoding region (200 bp). The molecular mass of the deduced protein is 33.9 kDa. Using the sequence of 3’UTR in EaCHS1 gene as primers, the real-time quantitative PCR result showed that the expression level of EaCHS1 gene was up regulated when induced by HHO except for in 1.0 mM of HHO. Southern blotting shows that EaCHS1 gene has four copies in E. adenophorum. We also constructed the over-expression vector of EaCHS1 gene and RNA interference vector which used the conservative sequence of CHS gene in E. adenophorum as target sequence respectively, and then transferred them into E. adenophorum and tobacco.
5. Arabidopsis Thaliana, as a kind of model plant, was used as receptor plant in order to preliminarily explore the root development affected by HHO. The result indicated that the growth of root hair was promoted in the root maturation zone of A. Thaliana when the concentration of HHO was 0.025 mM, whereas it was inhibited when the concentration of HHO was 0.1 mM and higher. This phenomenon showed that the growth of main root was promoted in a low concentration and inhibited in a high concentration of HHO.
引文
1.晏慧君,黄兴奇,程在全.cDNA文库构建策略及分析研究进展.云南农业大学学报,2006,21(1):1 ~ 6
2.柴强,黄高宝.植物化感作用的机理、影响因素及应用潜力.西北植物学报,2003,23 (3):509 ~ 515
3.陈晓亚,叶和春.植物次生代谢及其调控[A].见:李承森主编.植物科学进展(第1卷) [ C ].北京:高等教育出版社,1998,293 ~ 304
4.丁靖垲,余珍.紫茎泽兰精油的香气成分及应用研究[J].云南植物研究,1991,13(4):441 ~ 444
5.丁智慧,郭玉彬,丁靖恺.紫茎泽兰花的化学成分[J].云南植物研究,1999,21(4):505 ~ 51l
6.段惠,强胜,苏秀红,吴海荣,朱云枝,刘琳莉.用AFLP技术分析紫茎泽兰的遗传多样性.生态学报,2005,25 (8):2109 ~ 2114
7.冯玉龙,王跃华,刘元元,丁开宇.入侵物种飞机草和紫茎泽兰的核型研究.植物研究,2006,26(3):356 ~ 360
8.付洋,王洋,阎秀峰.萜类化合物的生理生态功能及经济价值[ J ].东北林业大学学报,2003,31 (6):59 ~ 62
9.高锦明.植物化学[M ].北京:科学出版社,2003
10.高贤明,唐廷贵,梁宇,郑天翔,桑卫国,陈艺林.外来植物黄顶菊的入侵警报及防控对策.生物多样性,2004,12(2):274 ~ 279
11.韩利红,冯玉龙.发育时期对紫茎泽兰化感作用的影响[J].生态学报,2007,27(3):1185 ~ 1191
12.郝立勤.关于加强控制紫茎泽兰疯狂蔓延危害的建议[J].科技发展研究,2001,4:23 ~ 25
13.和爱军,刘伦辉.紫茎泽兰浸提液对几种植物发芽的影响.杂草学报,1990,4(4):35 ~ 38
14.何萍,何永翔,陈莉.有害入侵植物—紫茎泽兰的综合治理.草业与畜牧,2006,8:27 ~ 29
15.何水林,林文雄,陈如凯,白镜焕,申东贤,金吉雄.辣椒倍半萜植保素代谢的分子生态学研究.农业生物技术学报,1999,7(4):377 ~ 381
16.侯太平,刘世贵.有毒植物紫茎泽兰研究进展.国外畜牧学—草原与牧草,1999,(4):6 ~ 8
17.黄益洪,周淼平,叶兴国,唐克轩,程红梅,陆维忠.农杆菌介导法获得小麦转基因植株的研究.作物学报,2002,28(4):510~515
18.黄文坤.紫茎泽兰群体遗传变异及化感作用相关基因的克隆.湖南农业大学博士学位论文,2007.
19.孔垂华,徐涛,胡飞.胜红蓟化感物质之间相互作用的研究.植物生态学报,1998,22(5):403 ~ 408
20.孔垂华,徐涛,胡飞.胜红蓟化感作用研究Ⅱ—主要化感物质的释放途径和活性.应用生态学报,1998,9(3):257 ~ 260
21.冷欣夫,邱星辉.细胞色素P450酶系的结构、功能与应用前景.北京:中国科学出版社,2001,3 ~ 5
22.李承森.植物科学进展(第一卷) [ C ].见:陈晓亚,叶和春编.植物次生代谢及其调控[A ].北京:高等教育出版社,1998
23.李丽,尹芳,张无敌.紫茎泽兰液和沼液抑制百合镰刀菌的研究[J].安徽农学通报,2007,13(3):136 ~ 137.
24.李蓉涛,丁智慧,丁靖垲.紫茎泽兰的化学成分[J].云南植物研究,1997,19(2):196 ~ 200
25.李双成,高江波.基于GARP模型的紫茎泽兰空间分布预测.生态学杂志,2008,27(9):1531 ~ 1536
26.李扬汉.中国杂草志.北京:中国农业出版社,1998
27.李云寿,邹华英,唐绍宗,杨益章,李晚谊,纳晓燕,佴注.14种菊科植物提取物对菜青虫的杀虫活性[J].华东昆虫学报,2000,9(2):99 ~ 101
28.林文雄,何华勤,郭春玉等.水稻化感作用及其生理生化特性的研究[J].应用生态学报,2001,12(6):871 ~ 875
29.刘栋,石强,李鹏丽,王宁宁.利用GUS基因瞬时表达对大豆子叶节和胚尖转化方法的比较及优化.生物学通报,2008,43(12):35 ~ 39
30.刘伦辉,刘文耀,郑征,荆桂芬.紫茎泽兰个体生物生态学特性研究.生态学报, 1989,9(1):66 ~ 70
31.刘文耀.紫茎泽兰的光合作用特征及其生态学意义.云南植物研究,1988,10(2):175 ~ 181
32.马利兵,王凤梅,潘建刚.Real-time PCR的研究进展及其应用.科技资讯,2008,31
33.毛建军,邱德文,杨秀芬,曾洪梅,袁京京.蛋白激发子基因pemG1转化三生烟及其对TMV抗性的提高.作物学报,2008,34(12):2070 ~ 2076
34.强胜.世界性恶性杂草──紫茎泽兰研究的历史及现状.武汉植物学研究,1998,16(4):354 ~ 365
35.瞿礼嘉等.植物生物化学与分子生物学.北京:科学出版社,2003,1057 ~ 1060
36. Sambrook J, Russell D W.分子克隆实验指南.北京:科学出版社,1993,611 ~ 618
37.宋启示,付昀,唐建维,冯志立,杨崇仁.紫茎泽兰的化学互感潜力.植物生态学报,2000,24(3):362 ~ 365
38.万方浩,郑小波,郭建英.重要农林外来入侵物种的生物学与控制.北京,科学出版社,2005,769 ~ 799
39.王东兰,郭建英,谢丙炎,万方浩,何龙飞.紫茎泽兰CYP75基因cDNA片段的克隆与鉴定.植物保护,2005,31 (4):65 ~ 69
40.王洪炯,何萍,马家林.紫茎泽兰传入凉山州草地调查研究报告.中国草地,1994,(1):62 ~ 64
41.王俊峰,冯玉龙.光强对两种入侵植物生物量分配、叶片形态和相对生长速率的影响.植物生态学报,2004,28 (6):781~786.
42.王世玉,郑用琏,刘亚,赵久然,张方东.玉米成熟胚胚性愈伤组织的诱导、高频再生及转化的研究.作物学报,2008,34(3):423 ~ 428
43.王亚玲,李群,何祖华.一个水稻P450CYP72基因簇受病原菌诱导和发育及组织特异性表达.科学通报,2003,48(21):2266 ~ 2270
44.王一丁,高平,张其红.紫茎泽兰灭蚜活性物质的分离、纯化及其对棉蚜乙酰胆碱脂酶的影响[J].高技术通讯,2002,(9):21~23
45.吴乃虎.基因工程原理.北京:科学出版社,1998,321 ~ 334
46.夏忠敏,金星,刘昌权.紫茎泽兰在贵州的发生危害情况及防除对策.植保技术与推广,2002,22(12):34 ~ 35
47.向业勋.紫茎泽兰的分布、危害及防除意见.杂草科学,1991,(4):10 ~ 11
48.许云龙,单欣宙,王宗玉.紫茎泽兰的化学成分初报[J].云南植物研究,1988,10(2):238 ~ 240
49.杨国庆.紫茎泽兰淋溶主效化感物质的分离鉴定及其对旱稻幼苗的作用机理.中国农业科学院博士学位论文,2006.
50.杨蓉西.危害严重的外来入侵—紫茎泽兰[J].生物学通报,2003,38 (1):20 ~ 21
51.于兴军,于丹,马克平.不同生境条件下紫茎泽兰化感作用的变化与入侵力关系的研究.植物生态学报,2004,28(6):773 ~ 780
52.赵国晶,马云萍.云南省紫茎泽兰的分布和危害的调查研究.杂草学报,1989,3(2):37 ~ 40
53.张培花,罗文富,杨艳丽.紫茎泽兰汁液及其萃取物对马铃薯晚疫病菌的抑制作用[J].西南农业学报,2006,19(2):246 ~ 250
54.赵宇玮,郝建国,步怀宇,王英娟,贾敬芬.青稞HvBADH1基因的克隆及其转化烟草的初步研究.作物学报,2008,34(7):1153 ~ 1159
55.郑丽,冯玉龙.紫茎泽兰叶片化感作用对10种草本植物种子萌发和幼苗生长的影响.生态学报,2005,25(10):2784 ~ 2787
56.朱正方,杨光忠,栗国强.紫茎泽兰化学成分的研究(I)[J].天然产物研究与开发,1997,9(3):35 ~ 39
57.祖元刚,王文杰,杨逢建,赵则海,曹建国.紫茎泽兰叶片气体交换的气孔调节特性:对其入侵能力的意义.林业科学,2005,41 (3):25 ~ 35
58. Abel S, Theologis A. Early genes and auxin action. Plant Physiol, 1996, 111: 9 ~ 17.
59. Abel S, Oeller P W, Theologis A. Early auxin-induced genes encode short-lived nuclear proteins. Proc.Natl.Acad.Sci, 1994, 91: 326 ~ 330.
60. Abdul-Wahab A S, Rice E L. Plant inhibition by Johnson grass and its possible significance in old-field succession. Bull Torrey Bot. Club, 1967, 94: 486 ~ 497.
61. Angiras N N, Singh S D, Singh C M. Allelopathic effects of some weeds on germination and growth of maize and soybean. Indian Journal of Weed Science, 1988, 20(2): 82 ~ 87.
62. Angiras N N, Singh S D, Singh C M. Allelopathic effects of some weeds on germination and growth of chickpea (Cicerariet). Indian Journal of Weed Science, 1989, 21, 85 ~ 87.
63. Arunachalam K, Arunachalam A, Melkania N P. Influence of soil properties on microbial populations, activity and biomass in humid subtropical mountainous ecosystems of India. Biol Fert Soils, 1999, 30: 217 ~ 223
64. Auld B A. Eupatorium weed species in Austrilia [J]. PestArticles and News Summaries, 1970, 16: 82 ~ 86.
65. Bach T J, Rogers D H, Rudney H. Detergent-solubilization, purification, and characterization of membrane-bound 3-hydroxy-3-methylglutaryl-coenzyme A reductase from radish seedlings. Eur J Biochem, 1986, 154 (1): 103 ~ 111
66. Bais H P, Vepachedu R, Gilroy S, Callaway R M, Vivanco J M. Allelopathy and exotic plants invasion: from molecules and genes to species interactions. Science, 2003, 301: 1377 ~ 1380.
67. Baker H G. The modes of origin of weeds [M]. / /Baker H G, Stebbines G L. The Genetics of Colonizing Species. New York: Academic Press, 1965: 147 ~ 168.
68. Ballas N, Wong L M, Theologis A. Identification of the auxin-responsive element, AuxRE, in the primary indoleacetic acid-inducible gene PS-IAA4/5 of pea (Pisum sativum). Proc.Natl.Acad.Sci, 1993, 92: 3483 ~ 3487.
69. Baruah N C, Sarma J C, Sarma S, Sharma R P. Seed germination and growth inhibitory cadinenes from Eupatorium adenophorum Spreng. Journal of Chemical Ecology, 1994, 20, 1885 ~ 1892.
70. Beres I, Kazinczi G. Allelopathic effects of shoot extracts and residues of weeds on field crops. Allelopathy Journal, 2000, 7(1): 93 ~ 98.
71. Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res, 1984, 12: 8711 ~ 8721.
72. Blum U. Effext of microbial utilization of phenolic acids and their phenolic acids break down products on allelopathic interaction [J]. Journal of Chemical Ecology, 1998, (24): 685 ~ 708
73. Bohlmann F, Gupta R K. Six cadinene derivatives from Ageratina Adenophora. Phytochemistry, 1981, 20 (6): 1432 ~ 1433.
74. Booker F L, Blum U, Fiscus E L. Short-term effects of ferulic acids on ion uptake and water relations on cucumber seedlings[J]. J. Exp. Bot., 1992, 43: 649 ~ 655
75. Bordoloi M J, Shakla V S, Sharam R P. Absolute stereochemistry of the insect antiffeedant cadinene from Eupatorinm adenophorum. Tetrahedron Letter, 1985, 26(4): 509 ~ 510.
76. Borthakur M, Dutta K, Nath SC, Singh RS. Micropropagation of Eclipta alba and Eupatorium adenophorum using a single-step nodal cutting technique. Plant Cell Tiss Org, 2000, 62: 239 ~ 242.
77. Brunner L, Luster J, Oche M, Davis J B. Phytotoixc effects of the high molecular weight fraction of an aqueous leaf litter extract on barley root development [J]. Plant and Soil, 1996, 178(1): 83 ~ 93.
78. Callaway R M, Aschehoug E T, Invasive plants versus their new and old neighbors: a mechanism for exotic invasion. Science, 2000, 290: 521 ~ 523.
79. Chappell J, Nable R. Induction of sesquiterpenoid biosynthesis in tobacco cell suspension cultures by fungal elicitor [J]. Plant Physiol, 1987,85: 469 ~ 473.
80. Chappell J, Wolf F, Proulx J, Cuellar R, Saunders C. Is the reaction catalysed by 3-hydroxy-3-methylglutaryl coenzyme A reductase a rate-limiting step for isoprenoid biosynthesis in plants? Plant Physiol, 1995, 109 (4): 1337 ~ 1343.
81. CHEN X Y, LIU P. Molecular biology and genetic engineering of plant secondary metabolism [J]. Life Science, 1996, 2 (8): 8211.
82. Choi D. Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in repose to Phytophthora infestans and to its elicitor arachidonic acid [J]. Plant Cell, 1992,4: 1333 ~ 1344.
83. Chou C H, Chang F J, Oka H I. Allelopathic potentials of wild rice oryza perennis[J]. Taiwania, 1991, 36(3): 201 ~ 210.
84. Christensen A B, Gregersen P L, Schroder J, Collinge D B. A chaleone synthase with an unusual substrate preference is expressed in barley Ieaves in response to UV-light and pathogen attack. Plant Mol. Biol, 1998, 37: 849 ~ 857.
85. Conner T W, Goekjian V H, Lafayette P R, Key J L. Structure and expression of two auxin-inducible genes from Arabidopsis. Plant Mol Biol, 1990, 15: 623 ~ 632.
86. Cruz O R, Anaya A L, Ramos L. Effects of allelopathic compounds of corn pollen on respiration and cell division of watermelon [J]. Journal of Chemical Ecology, 1988, 14(1): 71 ~ 86.
87. Dey S, Sinha B, Kalita J. Effect of Eupatorium adenophorum Spreng leaf extracts on the mustard aphid, Lipaphis erysimi Kalt: a scanning electron microscope study. Microsc Res Techniq, 2005, 66(1): 31 ~ 36.
88. Dixon R A, Paiva N L. Stress-induced phenylpropanoid metabolism [J]. Plant Cell, 1995, (7): 1085 ~ 1097.
89. Doyle J J. DNA protocols for plants-CTAB total DNA isolation. Hewitt G. Molecular technique in taxonomy. NATO ASI Series H, Cell Biology, 1991, 57: 283 ~ 293.
90. Duan H, Qiang S, Su X H, Wu H R, Zhu Y Z, Liu L L. Genetic diversity of Eupatorium adenophorum determined by AFLP marker. Acta Ecologica Sinica, 2004, 25(8): 2109 ~ 2114.
91. Durbin M L, MeCaig B, Clegg M T. Molecular evolution of chaleone synthase multigene fmily in the morning glory genome. Plant Mol. Biol, 2000, 42: 79 ~ 92.
92. Ebana K, Yan W G, Dilday R H, Namai H, Okuno K. Analysis of QYLs associated with the allelopathic effect of rice using water-soluble extracts. Breeding Science, 2001, 51 (1): 47 ~ 51.
93. El-Ghareeb R M. Suppression of annuals by Tribulus terrestris in an abandoned field in the sandy desert of Kuwait. J. Veg. Sci., 1991, 2: 147 ~ 154.
94. Enjuto M, Balcells L, Campos N. Arabidopsis thaliana contains two differentially expressed 3-hydroxy-3-methylglutaryl coenzyme A reductase genes, which encode microsomal forms of the enzyme. Proc Natl Acad Sci, 1994, 91: 927 ~ 931.
95. Estelle M. The plant hormone auxin: Insight in sight. Bioessays, 1992, 14: 439 ~ 443.
96. Feinbaum R L, Ausubel F M. Transcriptional regulateon of the Arabidopsis thaliana chalcone synthase gene [J]. Mol Cell Biol, 1988, 8: 1985 ~ 1992.
97. Feng Y L, Wang Y H, Liu Y Y, Ding K Y. Karyotype of Chromolaena odorata and Ageratina adenophora. Bulletin of Botanical Research, 2006, 26(3): 356 ~ 360.
98. Ferrer J L, Jez J M, Bowman M E, Dixon R A, Noel J P. Structure of chaleone synthase and the molecular basis of plant polyketide biosynthesis. Nat Struet Biol, 1999, 6(8): 775 ~ 84.
99. Flores-Benítez S, Jiménez~Bremont J F, Rosales-Mendoza S, Argüello-Astorga G R, Castillo-Collazo R, Alpuche-Solís AG. Genetic transformation of Agave salmiana by Agrobacterium tumefaciens and particle bombardment. Plant Cell Tiss Org, 2007, 91: 215 ~ 224.
100. Fraser A G, Kamath R S, Zipperlen P, Marnxa M C, Sohrmann M, Ahringer. J. Functional genomic analysis of C.elegans chromosome I by systematic RNA interference. Nature, 2000, 408: 325 ~ 330.
101. Gattas-Hallak A K, Davide L C, Souza L F. Effects of sorghum (Sorghub bicolor L.) root exudates on the cell cycle of the bean plant (Phaselus vulgaris.) root [J]. Genetics and Molecular Biology, 1999, 22(1): 95 ~ 99.
102. Greene D E, Johnson E A. Estimating the mean annual seed production of trees. Ecology, 1994, 75: 642 ~ 647.
103. Hagen G, Kleinscjmidt A J, Guilfoyle T J. Auxin-regulated gene expression in intact soybean hypocotyl and excised hypocotyl sections. Planta, 1984, 162: 147 ~ 153.
104. Hahlbrock K, Seheel D. Physiology and molecular Biology of phenylpropanoid metabolism. Ann Rev Plant Mol Biol, 1989, 40: 347 ~ 369.
105. Hajdukiewicz P, Svab Z, Maliga P. The small versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol, 1994, 25: 989 ~ 994.
106. Hassanein A, Chevreau E, Borion N. High efficient transformation of zonal (Pelargonium×hortorum) and scented (P. capitatum) geraniums via Agrobacterium tumefaciens using leaf discs. Plant Sci, 2005, 169: 532 ~ 541.
107. He S L, Zheng J G, Wang X F. Plant secondary metabolism, Function, reconcile, control and genetic engineering[J]. App lication and Environmental Biology, 2002, 8 (5): 558 ~ 563.
108. Hejl A M, Einhellig F A, Rasmussen J A. Effects of juglone on growth, photosynthesis, and respiration [J]. Journal of Chemical Ecology, 1993, 19(3): 559 ~ 568.
109. Herman I, Jacobs A, Van Montagu M, Depicker A. Plant chromosome/marker gene fusion assay for study of normal and truncated T-DNA integration events. Mol Gen Genet, 1990, 224: 248 ~ 256.
110. Herrmann A, Schulz W, Hahlbrock K. Two alleles of the single-copy chalcone synthase gene in Parsley differ by a transposen-like element [J]. Mol Gen Genet, 1988, 212: 93 ~ 98.
111. Hobbie L, Estelle M. Genetic approaches to auxin action. Plant Cell Environ, 1994, 17: 525 ~ 540.
112. Hwang B K. Effects of age-related resistance and metalaxyl on capsidiol production in pepper plants infected with phytophthora capsici. In: Handbook of phytoalexin metabolism and actin. (M.Daniel and R P Purkayastha eds), Marcel Dekker, Inc New York, 1995: 503 ~ 524.
113. Jefferson R A. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep,1987, 5: 387 ~ 405.
114. Jensen L B, Courtois B, Shen L, Li Z, Olofsdotter M, Mauleon R P. Locating genes controlling allelopathic effects against barnyardgrass in upland rice. Agronomy Journal, 2001, 93 (1): 261 ~ 264.
115. Jez J M, Noel J P. Meehanism of chalcone synthase: pKa of the catalytic cysteine and the role of the conserved histidine in a plant polyketide synthase. J Biol Chem, 2000, 275: 3960 ~ 3964.
116. JoséL B, Esmeralda H A, Lenin S C, et al. Phosphate Availability Alters Architecture and Causes Changes in Hormone Sensitivity in the Arabidopsis Root System. Plant Physiol, 2002, 129(1): 244 ~ 256.
117. Kaushal V, Dawra R K, Sharma O P, Kurade N P. Biochemical alterations in the blood plasma of rats associated with hepatotoxicity induced by Eupatorium adenophorum. Veterinary Research Communication, 2001, 25(7): 601 ~ 608.
118. Keil D J, Ludkow M A, Pinkava D J. Chromosome studies in Asteraceae from the United States, Mexico, the West Indies, and South America [J]. Am J Bot, 1988, 75: 652 ~ 668.
119. Kiran K, Kaul K. Autotoxicity in Tagetes erecta L. on its own germination and seedling growth. Allelophathy Journal, 2000, 7(1): 109 ~ 113.
120. Knogge W, Sehmelzer E, Weissenboek G. The role of chaleone synthase in the regulatjon of flavonoid biosynthesis in developingn oat primary leaves. Arch Biochem Biophys, 1986, 250: 364 ~ 372.
121. Koes R E, Spelt C E, Mol J N M, Gerats A G M. The chaleone synthase multigene family of Petunia hybrida: sequence homology, chromosomal location and evolutionary aspects. Plant Mol Biol, 1987, 10: 159 ~ 169.
122. Koes R E, Spelt C E, van den Elzen P J M, Mol J N M. Cloning and molecular charaeterization of the chalcone synthase multigene family of Petunia hybrida. Gene, 1989, 81: 245 ~ 257.
123. Koes R E, Quattrocehio F, Mol J N M. The flavonoid biosynthetic Pathway in Plants: funetion and evolution. Bioessays, 1994, 16: 123 ~ 132.
124. Kreuzaler F, Hahlbrock K. Ezymeic synthesis of aromatic compounds in higher plants: formation of naringenin (5, 7, 4’-rihydroxy-flavanone) from p-coumaroyl-coenzyme A and malonyl-coenzyme A. FEBS Lett, 1972, 28: 69 ~ 72.
125. Lanz T, Tropf S, Mamer F J, Sehroder J, Sehroder G. The role of cysteines in polyketide synthases. site direeted mutagenesis of resveratrol and chalcone synthases, two enzymes in different plant-specific pathways. J Biol Chem, 1991, 266: 9971 ~ 9976.
126. Li H, Qiang S, Cui J. Eupatroium adenophorum micro-propagation by bud and callus culture. Acta Bot Boreali-occident Sinica, 2005, 25 (7): 1458 ~ 1462.
127. Liu Z B, Ulmasov T, Shi X, Hagen G, Guilfoyle T J. The soybean GH3 promoter contains multiple auxin-inducible elements. Plant Cell, 1994, 6: 645 ~ 657.
128. Mahmoud S S, Croteau R B. Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase.Proc Natl Acad Sci USA, 2001, 98: 8915 ~ 8920.
129. Martin C R. Strueture, funetion, and regulation of the chaleone synthase in International Review of Cytology: A Survey of Cell Biology (K Jeon and J Jarvik, eds). Academic Press, 1993, PP: 233 ~ 284.
130. Mattice T, Lavy B, Skulman B. Searching for allelochemicals in rice that control ducksalad [A]. Olofsdotter M. Alleopathy in rice [C]. Manila, Philippines; IRRI, 1998.
131. Merlo L, Ghisi R, Rascio N. Effects of humic substances on carbohydrate metabolism of maize leaves [J]. Canadian Journal of Plant Science, 1991, 7(2): 419 ~ 425.
132. Molish H. Der Einflusseiner pflanze auf die andere-Allelopathie, Gustav Fischer Verlag, Jena, 1937.
133. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 1962, 15: 473 ~ 497
134. O’Sullivan B M. Investigations into Crofton weed (Eupatorium adenophorum) toxicity in horses. Aust Veter J, 1985, 62(1): 30 ~ 32.
135. Papes M, Peterson A T. Predicting the potential invasive distribution for Eupatorium adenophorum Spreng. Journal of Wuhan Botanical Research, 2003, 21:137 ~ 142.
136. Paxton J, Pocket botanical dictionary, 2nd ed. London: Bradbury and Evans, 1849.
137. Qiang S. The history and status of the study on croft on weed (Eupatorium adenophorum Spreng.) a worst worldwide weed. Journal of Wuhan Botanical Research, 1998, 16 (4): 366 ~ 372.
138. Ranjan S, Joanna K, Alan M, Daniel Z, et al. Structure-Function Analysis of the Presumptive Arabidopsis Auxin Permease AUX1. Plant Cell, 2004, 16(11): 3069 ~ 3083
139. Rebekah L, Gamble, X Q, Schaller G E. Mutational Analysis of the Ethylene Receptor ETR1. Role of the Histidine Kinase Domain in Dominant Ethylene Insensitivity. Plant Physiol, 2002, 128(4): 1428 ~ 1438.
140. Reimold U, Kroeger M, Kreuzaler F, Hahlbroek K. Coding and 3’noneoding nucleotide sequence of ehaleone synthase messenger RNA and assignment of amino acid sequence of the enzyme. EMBOJ, 1983, 2: 1801 ~ 1806.
141. Rice E L. Allelopathy. (Second Edition). New York: Academic Press, 1984, 23 ~ 28.
142. Ridenour W M, Callaway R M., The relative importance of allelopathy in interference: the effects of an invasive weed on a native bunchgrass. Oecologia, 2001, 126: 444 ~ 450.
143. Rizvi S J H, Rizvi V, Tahir M, Rahimian M H, Shimi P, Atri A. Genetic variation in allelopathic activity of wheat (Triticum aestivum L.) genotypes. Wheat Information Service, 2000, 91: 25 ~ 29.
144. Rohmer M, Knani M, Simonin P, Sutter B, Sahm H. Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate. Biochem J, 1993, 295: 517 ~ 524.
145. Ryder T B, Hedriek S A, Bell J N, Liang X, Clouse S D, Lamb C J. Organization and differential activation of a gene family encoding the Plant defense enzyme chaleone synthase in Phaseolus vulgaris. Mol Gen Genet, 1987, 210:219 ~ 233.
146. Sambrook J, Fritish E F, Manitis T. Molecular cloning: A laboratory Manaul, Second Edition (Coldspring harbor laboratory press, Cold spring harbor, NY).1987.
147. Sambrook, David W, Russell D W. Molecular Cloning: A Laboratory Mannual, 3rd ed. [M] New York: Cold Spring Harbor Laboratory Press, 2002, 549 ~ 552.
148. Sangeeta N, Maria G I, Gloria K M. Ethylene regulates lateral root formation and auxin transport in Arabidopsis thaliana. Plant J, 2008, 55(2): 175 ~ 187.
149. Sommer H, Saedler H. Structure of the chaleone synthase gene of Antirrhinum majus. Mol Gen Genet, 1986, 202: 429 ~ 434.
150. Song G Q, Loskutov A V, Sink K C. Highly efficient Agrobacterium tumefaciens-mediated transformation of celery (Apium graveolens L.) through somatic embryogenesis. Plant Cell Tiss Org, 2007, 88: 193 ~ 200.
151. Song Q S, Fu Y, Tang J W, Feng Z L, Yang C R. Allelopathic potential of Eupatorium adenophorum. Acta Phytoecologica Sinica, 2000, 24(3): 362 ~ 365.
152. Tel-Zur N, Abbo S, Mylaboski D, Mizrahi Y. Modified CTAB procedure for DNA isolation from epiphytic cacti of the Genera Hylocereus and Selenicereus (Cacataceae). Plant Mol Biol Rep, 1999, 17: 249 ~ 254.
153. Tripathi R S, Singh R S, Rai J P N. Allelopathic potential of Eupatorium adenophorum, a dominant ruderal weed of Meghalaya. Proceedings of Indian Academy of Sciences, 1981, 47: 458 ~ 465.
154. Tropf S, Kareher B, Sehroder G, Sehroder J. Reaction mechanisms of homodimeric plant polyketide synthases (stilbene and chaleone synthase). A single active site for the condensing reaction is suffieient for synthesis of stilbenes,chaleones,and 6’-deoxychaleones. J Biol Chem, 1995, 270: 7922 ~ 7928.
155. Van der Krol A R, Leniing P E, Veenstra J, van der Meer I M, Koes R E, Gerats A G M, Mol J N M, Stuitje A R. An anti-sense chaleone synthase gene in transgenic plants inhibits flower pigmentation. Nature, 1988, 333: 866 ~ 869.
156. Van der Krol A R, Mur L A, de Lange P, Gerats A G M, Mol J N M, Stuitje A R. Antisense chaleone synthase genes in petunia: visualization of variable transgene expression. Mol Gen Genet, 1990, 220: 204 ~ 212.
157. Vaughn S F, Berhow M A. Allelochemicals isolated from tissues of the invasive weed garlic mustard (Alliaria petiolata). Journal of Chemical Ecology, 1999, 25: 2495 ~ 2504.
158. Vogeli U, Chappell J. Induction of sesquiterpene cyclase and suppression of squalene synthelase activities in plant cell cultures treated with fungal elicitor. Plant Physiol, 1988, 88: 1291 ~ 1296.
159. Wang H J, He P, Ma J L. An Investigation and Research Report on the Dissemination of Ageratina adenophora on Rangeland Areas in Liangshan District of Sichuan Province. Grassland of China, 1994, 1: 62 ~ 64.
160. Wang R, Wang Y Z. Invasion dynamics and potential spread of the invasive alien plant species Ageratina adenophora (Asteraceae) in China. Diversity and Distributions, 2006, 12: 397 ~ 408.
161. WangY, Darnay B G, Rodwell V W. Identification of the principal catalytically important acidic residues of 3-hydroxy-3-methylglutaryl coenzyme A reductase. J Biol Chem, 1990, 265: 21634 ~21641.
162. Wienand U, Sommer H, Schwarz Z S. A general method to identify plant structureal genes amone genomics DNA clones using transposable element induced mutateons [J]. Mol Gen Genet, 1982, 187: 195 ~ 201.
163. Winkel-Shirtey B. Evdence for enzyme complexe in the phenylpropanoid and flavornoid pathway [J]. Physiol Plant, 1999, 107: 142 ~ 149.
164. Yan Y P, Wang Z Z. Genetic transformation of the medicinal plant Salvia miltiorrhiza by Agrobacterium tumefaciens~mediated method. Plant Cell Tiss Org, 2007, 88: 175 ~ 184.
165. Yang G Q, Wan F H, Liu W X, Zhang X W. Physiological effects of allelochemicals from leachates of Ageratina adenophora (Spreng.) on rice seedlings. Allelopathy Journal, 2006, 18 (2): 112 ~ 118.
166. Yang S F, Hoffman N E. Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol, 1984, 35: 155 ~ 189.Yin Z, Wang G L. Evidence of multiple complex patterns of T-DNA integration into the rice genome. Theor Appl Genet, 2000, 100: 461 ~ 470.
167. Yutaka O, Qianhong G C, Paul J O, Claudia K, Athanasios T. Age mutants of arabidopsis exhibit altered auxin-regulated gene expression. The Plant Cell, 1998, 10: 1649 ~ 1662.
168. Zhu Y Y, Machleder E M, Chenchik A. Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. Biotechniques, 2001, 30(4): 892 ~ 897.
2.柴强,黄高宝.植物化感作用的机理、影响因素及应用潜力.西北植物学报,2003,23 (3):509 ~ 515
3.陈晓亚,叶和春.植物次生代谢及其调控[A].见:李承森主编.植物科学进展(第1卷) [ C ].北京:高等教育出版社,1998,293 ~ 304
4.丁靖垲,余珍.紫茎泽兰精油的香气成分及应用研究[J].云南植物研究,1991,13(4):441 ~ 444
5.丁智慧,郭玉彬,丁靖恺.紫茎泽兰花的化学成分[J].云南植物研究,1999,21(4):505 ~ 51l
6.段惠,强胜,苏秀红,吴海荣,朱云枝,刘琳莉.用AFLP技术分析紫茎泽兰的遗传多样性.生态学报,2005,25 (8):2109 ~ 2114
7.冯玉龙,王跃华,刘元元,丁开宇.入侵物种飞机草和紫茎泽兰的核型研究.植物研究,2006,26(3):356 ~ 360
8.付洋,王洋,阎秀峰.萜类化合物的生理生态功能及经济价值[ J ].东北林业大学学报,2003,31 (6):59 ~ 62
9.高锦明.植物化学[M ].北京:科学出版社,2003
10.高贤明,唐廷贵,梁宇,郑天翔,桑卫国,陈艺林.外来植物黄顶菊的入侵警报及防控对策.生物多样性,2004,12(2):274 ~ 279
11.韩利红,冯玉龙.发育时期对紫茎泽兰化感作用的影响[J].生态学报,2007,27(3):1185 ~ 1191
12.郝立勤.关于加强控制紫茎泽兰疯狂蔓延危害的建议[J].科技发展研究,2001,4:23 ~ 25
13.和爱军,刘伦辉.紫茎泽兰浸提液对几种植物发芽的影响.杂草学报,1990,4(4):35 ~ 38
14.何萍,何永翔,陈莉.有害入侵植物—紫茎泽兰的综合治理.草业与畜牧,2006,8:27 ~ 29
15.何水林,林文雄,陈如凯,白镜焕,申东贤,金吉雄.辣椒倍半萜植保素代谢的分子生态学研究.农业生物技术学报,1999,7(4):377 ~ 381
16.侯太平,刘世贵.有毒植物紫茎泽兰研究进展.国外畜牧学—草原与牧草,1999,(4):6 ~ 8
17.黄益洪,周淼平,叶兴国,唐克轩,程红梅,陆维忠.农杆菌介导法获得小麦转基因植株的研究.作物学报,2002,28(4):510~515
18.黄文坤.紫茎泽兰群体遗传变异及化感作用相关基因的克隆.湖南农业大学博士学位论文,2007.
19.孔垂华,徐涛,胡飞.胜红蓟化感物质之间相互作用的研究.植物生态学报,1998,22(5):403 ~ 408
20.孔垂华,徐涛,胡飞.胜红蓟化感作用研究Ⅱ—主要化感物质的释放途径和活性.应用生态学报,1998,9(3):257 ~ 260
21.冷欣夫,邱星辉.细胞色素P450酶系的结构、功能与应用前景.北京:中国科学出版社,2001,3 ~ 5
22.李承森.植物科学进展(第一卷) [ C ].见:陈晓亚,叶和春编.植物次生代谢及其调控[A ].北京:高等教育出版社,1998
23.李丽,尹芳,张无敌.紫茎泽兰液和沼液抑制百合镰刀菌的研究[J].安徽农学通报,2007,13(3):136 ~ 137.
24.李蓉涛,丁智慧,丁靖垲.紫茎泽兰的化学成分[J].云南植物研究,1997,19(2):196 ~ 200
25.李双成,高江波.基于GARP模型的紫茎泽兰空间分布预测.生态学杂志,2008,27(9):1531 ~ 1536
26.李扬汉.中国杂草志.北京:中国农业出版社,1998
27.李云寿,邹华英,唐绍宗,杨益章,李晚谊,纳晓燕,佴注.14种菊科植物提取物对菜青虫的杀虫活性[J].华东昆虫学报,2000,9(2):99 ~ 101
28.林文雄,何华勤,郭春玉等.水稻化感作用及其生理生化特性的研究[J].应用生态学报,2001,12(6):871 ~ 875
29.刘栋,石强,李鹏丽,王宁宁.利用GUS基因瞬时表达对大豆子叶节和胚尖转化方法的比较及优化.生物学通报,2008,43(12):35 ~ 39
30.刘伦辉,刘文耀,郑征,荆桂芬.紫茎泽兰个体生物生态学特性研究.生态学报, 1989,9(1):66 ~ 70
31.刘文耀.紫茎泽兰的光合作用特征及其生态学意义.云南植物研究,1988,10(2):175 ~ 181
32.马利兵,王凤梅,潘建刚.Real-time PCR的研究进展及其应用.科技资讯,2008,31
33.毛建军,邱德文,杨秀芬,曾洪梅,袁京京.蛋白激发子基因pemG1转化三生烟及其对TMV抗性的提高.作物学报,2008,34(12):2070 ~ 2076
34.强胜.世界性恶性杂草──紫茎泽兰研究的历史及现状.武汉植物学研究,1998,16(4):354 ~ 365
35.瞿礼嘉等.植物生物化学与分子生物学.北京:科学出版社,2003,1057 ~ 1060
36. Sambrook J, Russell D W.分子克隆实验指南.北京:科学出版社,1993,611 ~ 618
37.宋启示,付昀,唐建维,冯志立,杨崇仁.紫茎泽兰的化学互感潜力.植物生态学报,2000,24(3):362 ~ 365
38.万方浩,郑小波,郭建英.重要农林外来入侵物种的生物学与控制.北京,科学出版社,2005,769 ~ 799
39.王东兰,郭建英,谢丙炎,万方浩,何龙飞.紫茎泽兰CYP75基因cDNA片段的克隆与鉴定.植物保护,2005,31 (4):65 ~ 69
40.王洪炯,何萍,马家林.紫茎泽兰传入凉山州草地调查研究报告.中国草地,1994,(1):62 ~ 64
41.王俊峰,冯玉龙.光强对两种入侵植物生物量分配、叶片形态和相对生长速率的影响.植物生态学报,2004,28 (6):781~786.
42.王世玉,郑用琏,刘亚,赵久然,张方东.玉米成熟胚胚性愈伤组织的诱导、高频再生及转化的研究.作物学报,2008,34(3):423 ~ 428
43.王亚玲,李群,何祖华.一个水稻P450CYP72基因簇受病原菌诱导和发育及组织特异性表达.科学通报,2003,48(21):2266 ~ 2270
44.王一丁,高平,张其红.紫茎泽兰灭蚜活性物质的分离、纯化及其对棉蚜乙酰胆碱脂酶的影响[J].高技术通讯,2002,(9):21~23
45.吴乃虎.基因工程原理.北京:科学出版社,1998,321 ~ 334
46.夏忠敏,金星,刘昌权.紫茎泽兰在贵州的发生危害情况及防除对策.植保技术与推广,2002,22(12):34 ~ 35
47.向业勋.紫茎泽兰的分布、危害及防除意见.杂草科学,1991,(4):10 ~ 11
48.许云龙,单欣宙,王宗玉.紫茎泽兰的化学成分初报[J].云南植物研究,1988,10(2):238 ~ 240
49.杨国庆.紫茎泽兰淋溶主效化感物质的分离鉴定及其对旱稻幼苗的作用机理.中国农业科学院博士学位论文,2006.
50.杨蓉西.危害严重的外来入侵—紫茎泽兰[J].生物学通报,2003,38 (1):20 ~ 21
51.于兴军,于丹,马克平.不同生境条件下紫茎泽兰化感作用的变化与入侵力关系的研究.植物生态学报,2004,28(6):773 ~ 780
52.赵国晶,马云萍.云南省紫茎泽兰的分布和危害的调查研究.杂草学报,1989,3(2):37 ~ 40
53.张培花,罗文富,杨艳丽.紫茎泽兰汁液及其萃取物对马铃薯晚疫病菌的抑制作用[J].西南农业学报,2006,19(2):246 ~ 250
54.赵宇玮,郝建国,步怀宇,王英娟,贾敬芬.青稞HvBADH1基因的克隆及其转化烟草的初步研究.作物学报,2008,34(7):1153 ~ 1159
55.郑丽,冯玉龙.紫茎泽兰叶片化感作用对10种草本植物种子萌发和幼苗生长的影响.生态学报,2005,25(10):2784 ~ 2787
56.朱正方,杨光忠,栗国强.紫茎泽兰化学成分的研究(I)[J].天然产物研究与开发,1997,9(3):35 ~ 39
57.祖元刚,王文杰,杨逢建,赵则海,曹建国.紫茎泽兰叶片气体交换的气孔调节特性:对其入侵能力的意义.林业科学,2005,41 (3):25 ~ 35
58. Abel S, Theologis A. Early genes and auxin action. Plant Physiol, 1996, 111: 9 ~ 17.
59. Abel S, Oeller P W, Theologis A. Early auxin-induced genes encode short-lived nuclear proteins. Proc.Natl.Acad.Sci, 1994, 91: 326 ~ 330.
60. Abdul-Wahab A S, Rice E L. Plant inhibition by Johnson grass and its possible significance in old-field succession. Bull Torrey Bot. Club, 1967, 94: 486 ~ 497.
61. Angiras N N, Singh S D, Singh C M. Allelopathic effects of some weeds on germination and growth of maize and soybean. Indian Journal of Weed Science, 1988, 20(2): 82 ~ 87.
62. Angiras N N, Singh S D, Singh C M. Allelopathic effects of some weeds on germination and growth of chickpea (Cicerariet). Indian Journal of Weed Science, 1989, 21, 85 ~ 87.
63. Arunachalam K, Arunachalam A, Melkania N P. Influence of soil properties on microbial populations, activity and biomass in humid subtropical mountainous ecosystems of India. Biol Fert Soils, 1999, 30: 217 ~ 223
64. Auld B A. Eupatorium weed species in Austrilia [J]. PestArticles and News Summaries, 1970, 16: 82 ~ 86.
65. Bach T J, Rogers D H, Rudney H. Detergent-solubilization, purification, and characterization of membrane-bound 3-hydroxy-3-methylglutaryl-coenzyme A reductase from radish seedlings. Eur J Biochem, 1986, 154 (1): 103 ~ 111
66. Bais H P, Vepachedu R, Gilroy S, Callaway R M, Vivanco J M. Allelopathy and exotic plants invasion: from molecules and genes to species interactions. Science, 2003, 301: 1377 ~ 1380.
67. Baker H G. The modes of origin of weeds [M]. / /Baker H G, Stebbines G L. The Genetics of Colonizing Species. New York: Academic Press, 1965: 147 ~ 168.
68. Ballas N, Wong L M, Theologis A. Identification of the auxin-responsive element, AuxRE, in the primary indoleacetic acid-inducible gene PS-IAA4/5 of pea (Pisum sativum). Proc.Natl.Acad.Sci, 1993, 92: 3483 ~ 3487.
69. Baruah N C, Sarma J C, Sarma S, Sharma R P. Seed germination and growth inhibitory cadinenes from Eupatorium adenophorum Spreng. Journal of Chemical Ecology, 1994, 20, 1885 ~ 1892.
70. Beres I, Kazinczi G. Allelopathic effects of shoot extracts and residues of weeds on field crops. Allelopathy Journal, 2000, 7(1): 93 ~ 98.
71. Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res, 1984, 12: 8711 ~ 8721.
72. Blum U. Effext of microbial utilization of phenolic acids and their phenolic acids break down products on allelopathic interaction [J]. Journal of Chemical Ecology, 1998, (24): 685 ~ 708
73. Bohlmann F, Gupta R K. Six cadinene derivatives from Ageratina Adenophora. Phytochemistry, 1981, 20 (6): 1432 ~ 1433.
74. Booker F L, Blum U, Fiscus E L. Short-term effects of ferulic acids on ion uptake and water relations on cucumber seedlings[J]. J. Exp. Bot., 1992, 43: 649 ~ 655
75. Bordoloi M J, Shakla V S, Sharam R P. Absolute stereochemistry of the insect antiffeedant cadinene from Eupatorinm adenophorum. Tetrahedron Letter, 1985, 26(4): 509 ~ 510.
76. Borthakur M, Dutta K, Nath SC, Singh RS. Micropropagation of Eclipta alba and Eupatorium adenophorum using a single-step nodal cutting technique. Plant Cell Tiss Org, 2000, 62: 239 ~ 242.
77. Brunner L, Luster J, Oche M, Davis J B. Phytotoixc effects of the high molecular weight fraction of an aqueous leaf litter extract on barley root development [J]. Plant and Soil, 1996, 178(1): 83 ~ 93.
78. Callaway R M, Aschehoug E T, Invasive plants versus their new and old neighbors: a mechanism for exotic invasion. Science, 2000, 290: 521 ~ 523.
79. Chappell J, Nable R. Induction of sesquiterpenoid biosynthesis in tobacco cell suspension cultures by fungal elicitor [J]. Plant Physiol, 1987,85: 469 ~ 473.
80. Chappell J, Wolf F, Proulx J, Cuellar R, Saunders C. Is the reaction catalysed by 3-hydroxy-3-methylglutaryl coenzyme A reductase a rate-limiting step for isoprenoid biosynthesis in plants? Plant Physiol, 1995, 109 (4): 1337 ~ 1343.
81. CHEN X Y, LIU P. Molecular biology and genetic engineering of plant secondary metabolism [J]. Life Science, 1996, 2 (8): 8211.
82. Choi D. Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in repose to Phytophthora infestans and to its elicitor arachidonic acid [J]. Plant Cell, 1992,4: 1333 ~ 1344.
83. Chou C H, Chang F J, Oka H I. Allelopathic potentials of wild rice oryza perennis[J]. Taiwania, 1991, 36(3): 201 ~ 210.
84. Christensen A B, Gregersen P L, Schroder J, Collinge D B. A chaleone synthase with an unusual substrate preference is expressed in barley Ieaves in response to UV-light and pathogen attack. Plant Mol. Biol, 1998, 37: 849 ~ 857.
85. Conner T W, Goekjian V H, Lafayette P R, Key J L. Structure and expression of two auxin-inducible genes from Arabidopsis. Plant Mol Biol, 1990, 15: 623 ~ 632.
86. Cruz O R, Anaya A L, Ramos L. Effects of allelopathic compounds of corn pollen on respiration and cell division of watermelon [J]. Journal of Chemical Ecology, 1988, 14(1): 71 ~ 86.
87. Dey S, Sinha B, Kalita J. Effect of Eupatorium adenophorum Spreng leaf extracts on the mustard aphid, Lipaphis erysimi Kalt: a scanning electron microscope study. Microsc Res Techniq, 2005, 66(1): 31 ~ 36.
88. Dixon R A, Paiva N L. Stress-induced phenylpropanoid metabolism [J]. Plant Cell, 1995, (7): 1085 ~ 1097.
89. Doyle J J. DNA protocols for plants-CTAB total DNA isolation. Hewitt G. Molecular technique in taxonomy. NATO ASI Series H, Cell Biology, 1991, 57: 283 ~ 293.
90. Duan H, Qiang S, Su X H, Wu H R, Zhu Y Z, Liu L L. Genetic diversity of Eupatorium adenophorum determined by AFLP marker. Acta Ecologica Sinica, 2004, 25(8): 2109 ~ 2114.
91. Durbin M L, MeCaig B, Clegg M T. Molecular evolution of chaleone synthase multigene fmily in the morning glory genome. Plant Mol. Biol, 2000, 42: 79 ~ 92.
92. Ebana K, Yan W G, Dilday R H, Namai H, Okuno K. Analysis of QYLs associated with the allelopathic effect of rice using water-soluble extracts. Breeding Science, 2001, 51 (1): 47 ~ 51.
93. El-Ghareeb R M. Suppression of annuals by Tribulus terrestris in an abandoned field in the sandy desert of Kuwait. J. Veg. Sci., 1991, 2: 147 ~ 154.
94. Enjuto M, Balcells L, Campos N. Arabidopsis thaliana contains two differentially expressed 3-hydroxy-3-methylglutaryl coenzyme A reductase genes, which encode microsomal forms of the enzyme. Proc Natl Acad Sci, 1994, 91: 927 ~ 931.
95. Estelle M. The plant hormone auxin: Insight in sight. Bioessays, 1992, 14: 439 ~ 443.
96. Feinbaum R L, Ausubel F M. Transcriptional regulateon of the Arabidopsis thaliana chalcone synthase gene [J]. Mol Cell Biol, 1988, 8: 1985 ~ 1992.
97. Feng Y L, Wang Y H, Liu Y Y, Ding K Y. Karyotype of Chromolaena odorata and Ageratina adenophora. Bulletin of Botanical Research, 2006, 26(3): 356 ~ 360.
98. Ferrer J L, Jez J M, Bowman M E, Dixon R A, Noel J P. Structure of chaleone synthase and the molecular basis of plant polyketide biosynthesis. Nat Struet Biol, 1999, 6(8): 775 ~ 84.
99. Flores-Benítez S, Jiménez~Bremont J F, Rosales-Mendoza S, Argüello-Astorga G R, Castillo-Collazo R, Alpuche-Solís AG. Genetic transformation of Agave salmiana by Agrobacterium tumefaciens and particle bombardment. Plant Cell Tiss Org, 2007, 91: 215 ~ 224.
100. Fraser A G, Kamath R S, Zipperlen P, Marnxa M C, Sohrmann M, Ahringer. J. Functional genomic analysis of C.elegans chromosome I by systematic RNA interference. Nature, 2000, 408: 325 ~ 330.
101. Gattas-Hallak A K, Davide L C, Souza L F. Effects of sorghum (Sorghub bicolor L.) root exudates on the cell cycle of the bean plant (Phaselus vulgaris.) root [J]. Genetics and Molecular Biology, 1999, 22(1): 95 ~ 99.
102. Greene D E, Johnson E A. Estimating the mean annual seed production of trees. Ecology, 1994, 75: 642 ~ 647.
103. Hagen G, Kleinscjmidt A J, Guilfoyle T J. Auxin-regulated gene expression in intact soybean hypocotyl and excised hypocotyl sections. Planta, 1984, 162: 147 ~ 153.
104. Hahlbrock K, Seheel D. Physiology and molecular Biology of phenylpropanoid metabolism. Ann Rev Plant Mol Biol, 1989, 40: 347 ~ 369.
105. Hajdukiewicz P, Svab Z, Maliga P. The small versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol, 1994, 25: 989 ~ 994.
106. Hassanein A, Chevreau E, Borion N. High efficient transformation of zonal (Pelargonium×hortorum) and scented (P. capitatum) geraniums via Agrobacterium tumefaciens using leaf discs. Plant Sci, 2005, 169: 532 ~ 541.
107. He S L, Zheng J G, Wang X F. Plant secondary metabolism, Function, reconcile, control and genetic engineering[J]. App lication and Environmental Biology, 2002, 8 (5): 558 ~ 563.
108. Hejl A M, Einhellig F A, Rasmussen J A. Effects of juglone on growth, photosynthesis, and respiration [J]. Journal of Chemical Ecology, 1993, 19(3): 559 ~ 568.
109. Herman I, Jacobs A, Van Montagu M, Depicker A. Plant chromosome/marker gene fusion assay for study of normal and truncated T-DNA integration events. Mol Gen Genet, 1990, 224: 248 ~ 256.
110. Herrmann A, Schulz W, Hahlbrock K. Two alleles of the single-copy chalcone synthase gene in Parsley differ by a transposen-like element [J]. Mol Gen Genet, 1988, 212: 93 ~ 98.
111. Hobbie L, Estelle M. Genetic approaches to auxin action. Plant Cell Environ, 1994, 17: 525 ~ 540.
112. Hwang B K. Effects of age-related resistance and metalaxyl on capsidiol production in pepper plants infected with phytophthora capsici. In: Handbook of phytoalexin metabolism and actin. (M.Daniel and R P Purkayastha eds), Marcel Dekker, Inc New York, 1995: 503 ~ 524.
113. Jefferson R A. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep,1987, 5: 387 ~ 405.
114. Jensen L B, Courtois B, Shen L, Li Z, Olofsdotter M, Mauleon R P. Locating genes controlling allelopathic effects against barnyardgrass in upland rice. Agronomy Journal, 2001, 93 (1): 261 ~ 264.
115. Jez J M, Noel J P. Meehanism of chalcone synthase: pKa of the catalytic cysteine and the role of the conserved histidine in a plant polyketide synthase. J Biol Chem, 2000, 275: 3960 ~ 3964.
116. JoséL B, Esmeralda H A, Lenin S C, et al. Phosphate Availability Alters Architecture and Causes Changes in Hormone Sensitivity in the Arabidopsis Root System. Plant Physiol, 2002, 129(1): 244 ~ 256.
117. Kaushal V, Dawra R K, Sharma O P, Kurade N P. Biochemical alterations in the blood plasma of rats associated with hepatotoxicity induced by Eupatorium adenophorum. Veterinary Research Communication, 2001, 25(7): 601 ~ 608.
118. Keil D J, Ludkow M A, Pinkava D J. Chromosome studies in Asteraceae from the United States, Mexico, the West Indies, and South America [J]. Am J Bot, 1988, 75: 652 ~ 668.
119. Kiran K, Kaul K. Autotoxicity in Tagetes erecta L. on its own germination and seedling growth. Allelophathy Journal, 2000, 7(1): 109 ~ 113.
120. Knogge W, Sehmelzer E, Weissenboek G. The role of chaleone synthase in the regulatjon of flavonoid biosynthesis in developingn oat primary leaves. Arch Biochem Biophys, 1986, 250: 364 ~ 372.
121. Koes R E, Spelt C E, Mol J N M, Gerats A G M. The chaleone synthase multigene family of Petunia hybrida: sequence homology, chromosomal location and evolutionary aspects. Plant Mol Biol, 1987, 10: 159 ~ 169.
122. Koes R E, Spelt C E, van den Elzen P J M, Mol J N M. Cloning and molecular charaeterization of the chalcone synthase multigene family of Petunia hybrida. Gene, 1989, 81: 245 ~ 257.
123. Koes R E, Quattrocehio F, Mol J N M. The flavonoid biosynthetic Pathway in Plants: funetion and evolution. Bioessays, 1994, 16: 123 ~ 132.
124. Kreuzaler F, Hahlbrock K. Ezymeic synthesis of aromatic compounds in higher plants: formation of naringenin (5, 7, 4’-rihydroxy-flavanone) from p-coumaroyl-coenzyme A and malonyl-coenzyme A. FEBS Lett, 1972, 28: 69 ~ 72.
125. Lanz T, Tropf S, Mamer F J, Sehroder J, Sehroder G. The role of cysteines in polyketide synthases. site direeted mutagenesis of resveratrol and chalcone synthases, two enzymes in different plant-specific pathways. J Biol Chem, 1991, 266: 9971 ~ 9976.
126. Li H, Qiang S, Cui J. Eupatroium adenophorum micro-propagation by bud and callus culture. Acta Bot Boreali-occident Sinica, 2005, 25 (7): 1458 ~ 1462.
127. Liu Z B, Ulmasov T, Shi X, Hagen G, Guilfoyle T J. The soybean GH3 promoter contains multiple auxin-inducible elements. Plant Cell, 1994, 6: 645 ~ 657.
128. Mahmoud S S, Croteau R B. Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase.Proc Natl Acad Sci USA, 2001, 98: 8915 ~ 8920.
129. Martin C R. Strueture, funetion, and regulation of the chaleone synthase in International Review of Cytology: A Survey of Cell Biology (K Jeon and J Jarvik, eds). Academic Press, 1993, PP: 233 ~ 284.
130. Mattice T, Lavy B, Skulman B. Searching for allelochemicals in rice that control ducksalad [A]. Olofsdotter M. Alleopathy in rice [C]. Manila, Philippines; IRRI, 1998.
131. Merlo L, Ghisi R, Rascio N. Effects of humic substances on carbohydrate metabolism of maize leaves [J]. Canadian Journal of Plant Science, 1991, 7(2): 419 ~ 425.
132. Molish H. Der Einflusseiner pflanze auf die andere-Allelopathie, Gustav Fischer Verlag, Jena, 1937.
133. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 1962, 15: 473 ~ 497
134. O’Sullivan B M. Investigations into Crofton weed (Eupatorium adenophorum) toxicity in horses. Aust Veter J, 1985, 62(1): 30 ~ 32.
135. Papes M, Peterson A T. Predicting the potential invasive distribution for Eupatorium adenophorum Spreng. Journal of Wuhan Botanical Research, 2003, 21:137 ~ 142.
136. Paxton J, Pocket botanical dictionary, 2nd ed. London: Bradbury and Evans, 1849.
137. Qiang S. The history and status of the study on croft on weed (Eupatorium adenophorum Spreng.) a worst worldwide weed. Journal of Wuhan Botanical Research, 1998, 16 (4): 366 ~ 372.
138. Ranjan S, Joanna K, Alan M, Daniel Z, et al. Structure-Function Analysis of the Presumptive Arabidopsis Auxin Permease AUX1. Plant Cell, 2004, 16(11): 3069 ~ 3083
139. Rebekah L, Gamble, X Q, Schaller G E. Mutational Analysis of the Ethylene Receptor ETR1. Role of the Histidine Kinase Domain in Dominant Ethylene Insensitivity. Plant Physiol, 2002, 128(4): 1428 ~ 1438.
140. Reimold U, Kroeger M, Kreuzaler F, Hahlbroek K. Coding and 3’noneoding nucleotide sequence of ehaleone synthase messenger RNA and assignment of amino acid sequence of the enzyme. EMBOJ, 1983, 2: 1801 ~ 1806.
141. Rice E L. Allelopathy. (Second Edition). New York: Academic Press, 1984, 23 ~ 28.
142. Ridenour W M, Callaway R M., The relative importance of allelopathy in interference: the effects of an invasive weed on a native bunchgrass. Oecologia, 2001, 126: 444 ~ 450.
143. Rizvi S J H, Rizvi V, Tahir M, Rahimian M H, Shimi P, Atri A. Genetic variation in allelopathic activity of wheat (Triticum aestivum L.) genotypes. Wheat Information Service, 2000, 91: 25 ~ 29.
144. Rohmer M, Knani M, Simonin P, Sutter B, Sahm H. Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate. Biochem J, 1993, 295: 517 ~ 524.
145. Ryder T B, Hedriek S A, Bell J N, Liang X, Clouse S D, Lamb C J. Organization and differential activation of a gene family encoding the Plant defense enzyme chaleone synthase in Phaseolus vulgaris. Mol Gen Genet, 1987, 210:219 ~ 233.
146. Sambrook J, Fritish E F, Manitis T. Molecular cloning: A laboratory Manaul, Second Edition (Coldspring harbor laboratory press, Cold spring harbor, NY).1987.
147. Sambrook, David W, Russell D W. Molecular Cloning: A Laboratory Mannual, 3rd ed. [M] New York: Cold Spring Harbor Laboratory Press, 2002, 549 ~ 552.
148. Sangeeta N, Maria G I, Gloria K M. Ethylene regulates lateral root formation and auxin transport in Arabidopsis thaliana. Plant J, 2008, 55(2): 175 ~ 187.
149. Sommer H, Saedler H. Structure of the chaleone synthase gene of Antirrhinum majus. Mol Gen Genet, 1986, 202: 429 ~ 434.
150. Song G Q, Loskutov A V, Sink K C. Highly efficient Agrobacterium tumefaciens-mediated transformation of celery (Apium graveolens L.) through somatic embryogenesis. Plant Cell Tiss Org, 2007, 88: 193 ~ 200.
151. Song Q S, Fu Y, Tang J W, Feng Z L, Yang C R. Allelopathic potential of Eupatorium adenophorum. Acta Phytoecologica Sinica, 2000, 24(3): 362 ~ 365.
152. Tel-Zur N, Abbo S, Mylaboski D, Mizrahi Y. Modified CTAB procedure for DNA isolation from epiphytic cacti of the Genera Hylocereus and Selenicereus (Cacataceae). Plant Mol Biol Rep, 1999, 17: 249 ~ 254.
153. Tripathi R S, Singh R S, Rai J P N. Allelopathic potential of Eupatorium adenophorum, a dominant ruderal weed of Meghalaya. Proceedings of Indian Academy of Sciences, 1981, 47: 458 ~ 465.
154. Tropf S, Kareher B, Sehroder G, Sehroder J. Reaction mechanisms of homodimeric plant polyketide synthases (stilbene and chaleone synthase). A single active site for the condensing reaction is suffieient for synthesis of stilbenes,chaleones,and 6’-deoxychaleones. J Biol Chem, 1995, 270: 7922 ~ 7928.
155. Van der Krol A R, Leniing P E, Veenstra J, van der Meer I M, Koes R E, Gerats A G M, Mol J N M, Stuitje A R. An anti-sense chaleone synthase gene in transgenic plants inhibits flower pigmentation. Nature, 1988, 333: 866 ~ 869.
156. Van der Krol A R, Mur L A, de Lange P, Gerats A G M, Mol J N M, Stuitje A R. Antisense chaleone synthase genes in petunia: visualization of variable transgene expression. Mol Gen Genet, 1990, 220: 204 ~ 212.
157. Vaughn S F, Berhow M A. Allelochemicals isolated from tissues of the invasive weed garlic mustard (Alliaria petiolata). Journal of Chemical Ecology, 1999, 25: 2495 ~ 2504.
158. Vogeli U, Chappell J. Induction of sesquiterpene cyclase and suppression of squalene synthelase activities in plant cell cultures treated with fungal elicitor. Plant Physiol, 1988, 88: 1291 ~ 1296.
159. Wang H J, He P, Ma J L. An Investigation and Research Report on the Dissemination of Ageratina adenophora on Rangeland Areas in Liangshan District of Sichuan Province. Grassland of China, 1994, 1: 62 ~ 64.
160. Wang R, Wang Y Z. Invasion dynamics and potential spread of the invasive alien plant species Ageratina adenophora (Asteraceae) in China. Diversity and Distributions, 2006, 12: 397 ~ 408.
161. WangY, Darnay B G, Rodwell V W. Identification of the principal catalytically important acidic residues of 3-hydroxy-3-methylglutaryl coenzyme A reductase. J Biol Chem, 1990, 265: 21634 ~21641.
162. Wienand U, Sommer H, Schwarz Z S. A general method to identify plant structureal genes amone genomics DNA clones using transposable element induced mutateons [J]. Mol Gen Genet, 1982, 187: 195 ~ 201.
163. Winkel-Shirtey B. Evdence for enzyme complexe in the phenylpropanoid and flavornoid pathway [J]. Physiol Plant, 1999, 107: 142 ~ 149.
164. Yan Y P, Wang Z Z. Genetic transformation of the medicinal plant Salvia miltiorrhiza by Agrobacterium tumefaciens~mediated method. Plant Cell Tiss Org, 2007, 88: 175 ~ 184.
165. Yang G Q, Wan F H, Liu W X, Zhang X W. Physiological effects of allelochemicals from leachates of Ageratina adenophora (Spreng.) on rice seedlings. Allelopathy Journal, 2006, 18 (2): 112 ~ 118.
166. Yang S F, Hoffman N E. Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol, 1984, 35: 155 ~ 189.Yin Z, Wang G L. Evidence of multiple complex patterns of T-DNA integration into the rice genome. Theor Appl Genet, 2000, 100: 461 ~ 470.
167. Yutaka O, Qianhong G C, Paul J O, Claudia K, Athanasios T. Age mutants of arabidopsis exhibit altered auxin-regulated gene expression. The Plant Cell, 1998, 10: 1649 ~ 1662.
168. Zhu Y Y, Machleder E M, Chenchik A. Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. Biotechniques, 2001, 30(4): 892 ~ 897.
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