绵羊瘤胃乙酸和丁酸的产生、吸收和利用规律及可代谢生脂物质（MLS）的测定
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摘要
本论文包括两个部分:
第一部分:基础日粮条件下,绵羊瘤胃乙酸、丁酸的产生、吸收和利用规律及可代谢生脂物质(MLS)的测定
试验一:基础日粮条件下,绵羊瘤胃乙酸﹑丁酸的产生﹑吸收规律及MLS测定本试验选用六只体况良好、1.5岁左右、体重为29-29.2 kg的内蒙古半细毛羯羊,安装有永久性瘤胃瘘管和近端十二指肠瘘管,通过模拟连续饲喂从而获得瘤胃稳态条件下,借助乙酸、丁酸溶液的启动-连续灌注,研究基础日粮条件下瘤胃乙酸、丁酸的产生、吸收和流通规律,并计算MLS值,结果如下:(1)基础日粮条件下,试验羊瘤胃乙酸﹑丁酸的基本产生速率分别为61.66 mmol/h和23.04 mmol/h;吸收速率分别为47.15 mmol/h和17.86 mmol/h,流通速率分别为14.52mmol/h和5.19mmol/h,吸收率分别为76.33%和77.52%。(2)瘤胃乙酸、丁酸的产生速率(X, mmol/h)与吸收速率(Y, mmol/h)的回归方程分别为:乙酸: Y=0.6905X+4.4554(R~2=0.9973,n=5),丁酸:Y=0.1514X+14.311(R~2=0.8227,n=5);瘤胃乙酸、丁酸的产生速率(X, mmol/h)与乙酸、丁酸瘤胃浓度(Y, mmol/L)的回归方程分别为:乙酸: y = 0.3739x + 5.4456(R~2 = 0.9899,n=5),丁酸: y = 0.3617x + 1.8599(R2 = 0.9247,n=5)。(3)本试验测出基础日粮每日提供可代谢生脂物质为135.69g,每日提供代谢葡萄糖43.83g,基础日粮MLS/MG值为3.10, C2+C4能、C3+C6能分别占日粮ME的89.24%和11.30%。
试验二:基础日粮条件下,绵羊瘤胃乙酸和丁酸全内脏组织利用规律的研究本试验选用三只体况良好,体重29-31 kg的1.5岁左右内蒙古半细毛羯羊,安装有肝静脉、门静脉、肠系膜静脉、颈动脉慢性血插管。通过模拟连续饲喂从而获得瘤胃稳态条件下,研究试验羊全内脏组织对乙酸、丁酸的利用。结果如下:基础日粮条件下,试验羊MDV、PDV及肝脏的乙酸流量分别为5.55、19.12和1.23 mmol/h,MDV、PDV和肝脏各消耗瘤胃乙酸产生量的9%、31%和2%;而MDV、PDV及肝脏的丁酸流量分别为1.38、21.43和1.41mmol/h,MDV、PDV和肝脏分别消耗瘤胃丁酸产生量的6%、93%和6%。
第二部分:瘤胃乙酸﹑丁酸产生量的提高对其消化道吸收和流通的影响本试验选用六只体况良好、安装有永久性瘤胃瘘管的内蒙古半细毛羯羊,试验安排三个处理,每个处理代表一个灌注的乙酸﹑丁酸水平及一次启动-连续灌注试验,通过模拟连续饲喂从而获得瘤胃稳态条件下,研究瘤胃乙酸、丁酸产生量的提高对其消化道吸收流通的影响。结果表明:(1)随着乙酸产生速率的提高,瘤胃pH、乙酸吸收率显著下降(P<0.01),乙酸浓度、乙酸吸收速率和乙酸流通速率显著上升
This paper consisted of two parts:
Part1: Study on the production, absorption and utilization of acetate and butyrate in the rumen of sheep and measurement of dietary MLS
Exp1: The production and absorption of acetate and butyrate in the rumen of sheep and the measurement of MLS
Six Inner Mongolian semifine-wool wethers (1.5 old years, 29-29.2 kg BW) fed a 1.0 M basal diet and fitted with a ruminal and a duodenum cannula were used to study the production, absorption, passage and utilization of acetate and butyrate in the rumen by simulating continuous feeding and pulse-continuous infusion technology. Rumen steady state was obtained through eight equal portions feeding daily, and dietary metabolizable lipogenous substances (MLS) was calculated subsequently. The results showed that: (1) Under the basal diet condition , the principal production rate of acetate and butyrate in the rumen of sheep were 61.66 mmol/h and 23.04 mmol/h respectively; the absorption rate of acetate and butyrate were 47.86 mmol/h and 17.86 mmol/h respectively; the passage rate of acetate and butyrate were 14.52 mmol/h and 5.19 mmol/h respectively; the fractional absorption rate of acetate and butyrate were 76.33% and 77.52% respectively. (2) the regression equation between the principal production rate (X, mmol/h)and the absorption rate of acetate and butyrate(Y, mmol/h) in the rumen of sheep were as follows: for acetate: Y=0.6905X+4.4554(R2=0.9973,n=5),for butyrate: Y=0.1514X+14.311(R2=0.8227,n=5);the regression equation between the production rate of acetate and butyrate(X, mmol/h) in the rumen of sheep and the rumen concentration of acetate and butyrate(Y, mmol/L) were as follows: for acetate: y = 0.3739x + 5.4456(R2 = 0.9899, n=5), for butyrate: y = 0.3617x + 1.8599(R2 = 0.9247,n=5.)(3)Dietary MLS and MG value were 135.69g and 43.83g respectively; the ratio of MLS/MG was 3.10; the ratio of C2+C4 energy and of C3+C6 energy in dietary ME were 89.24%和11.30% respectively.
Exp2: The utilization of acetate and butyrate in the rumen in splanchnic tissues of the sheep
Three inner Mongolian semifine-wool wethers (1.5 old years, 29-31kg BW) fed 1.0 M basal diet and fitted with chronic indwelling catheters in the mesenteric vein, hepatic
第一部分:基础日粮条件下,绵羊瘤胃乙酸、丁酸的产生、吸收和利用规律及可代谢生脂物质(MLS)的测定
试验一:基础日粮条件下,绵羊瘤胃乙酸﹑丁酸的产生﹑吸收规律及MLS测定本试验选用六只体况良好、1.5岁左右、体重为29-29.2 kg的内蒙古半细毛羯羊,安装有永久性瘤胃瘘管和近端十二指肠瘘管,通过模拟连续饲喂从而获得瘤胃稳态条件下,借助乙酸、丁酸溶液的启动-连续灌注,研究基础日粮条件下瘤胃乙酸、丁酸的产生、吸收和流通规律,并计算MLS值,结果如下:(1)基础日粮条件下,试验羊瘤胃乙酸﹑丁酸的基本产生速率分别为61.66 mmol/h和23.04 mmol/h;吸收速率分别为47.15 mmol/h和17.86 mmol/h,流通速率分别为14.52mmol/h和5.19mmol/h,吸收率分别为76.33%和77.52%。(2)瘤胃乙酸、丁酸的产生速率(X, mmol/h)与吸收速率(Y, mmol/h)的回归方程分别为:乙酸: Y=0.6905X+4.4554(R~2=0.9973,n=5),丁酸:Y=0.1514X+14.311(R~2=0.8227,n=5);瘤胃乙酸、丁酸的产生速率(X, mmol/h)与乙酸、丁酸瘤胃浓度(Y, mmol/L)的回归方程分别为:乙酸: y = 0.3739x + 5.4456(R~2 = 0.9899,n=5),丁酸: y = 0.3617x + 1.8599(R2 = 0.9247,n=5)。(3)本试验测出基础日粮每日提供可代谢生脂物质为135.69g,每日提供代谢葡萄糖43.83g,基础日粮MLS/MG值为3.10, C2+C4能、C3+C6能分别占日粮ME的89.24%和11.30%。
试验二:基础日粮条件下,绵羊瘤胃乙酸和丁酸全内脏组织利用规律的研究本试验选用三只体况良好,体重29-31 kg的1.5岁左右内蒙古半细毛羯羊,安装有肝静脉、门静脉、肠系膜静脉、颈动脉慢性血插管。通过模拟连续饲喂从而获得瘤胃稳态条件下,研究试验羊全内脏组织对乙酸、丁酸的利用。结果如下:基础日粮条件下,试验羊MDV、PDV及肝脏的乙酸流量分别为5.55、19.12和1.23 mmol/h,MDV、PDV和肝脏各消耗瘤胃乙酸产生量的9%、31%和2%;而MDV、PDV及肝脏的丁酸流量分别为1.38、21.43和1.41mmol/h,MDV、PDV和肝脏分别消耗瘤胃丁酸产生量的6%、93%和6%。
第二部分:瘤胃乙酸﹑丁酸产生量的提高对其消化道吸收和流通的影响本试验选用六只体况良好、安装有永久性瘤胃瘘管的内蒙古半细毛羯羊,试验安排三个处理,每个处理代表一个灌注的乙酸﹑丁酸水平及一次启动-连续灌注试验,通过模拟连续饲喂从而获得瘤胃稳态条件下,研究瘤胃乙酸、丁酸产生量的提高对其消化道吸收流通的影响。结果表明:(1)随着乙酸产生速率的提高,瘤胃pH、乙酸吸收率显著下降(P<0.01),乙酸浓度、乙酸吸收速率和乙酸流通速率显著上升
This paper consisted of two parts:
Part1: Study on the production, absorption and utilization of acetate and butyrate in the rumen of sheep and measurement of dietary MLS
Exp1: The production and absorption of acetate and butyrate in the rumen of sheep and the measurement of MLS
Six Inner Mongolian semifine-wool wethers (1.5 old years, 29-29.2 kg BW) fed a 1.0 M basal diet and fitted with a ruminal and a duodenum cannula were used to study the production, absorption, passage and utilization of acetate and butyrate in the rumen by simulating continuous feeding and pulse-continuous infusion technology. Rumen steady state was obtained through eight equal portions feeding daily, and dietary metabolizable lipogenous substances (MLS) was calculated subsequently. The results showed that: (1) Under the basal diet condition , the principal production rate of acetate and butyrate in the rumen of sheep were 61.66 mmol/h and 23.04 mmol/h respectively; the absorption rate of acetate and butyrate were 47.86 mmol/h and 17.86 mmol/h respectively; the passage rate of acetate and butyrate were 14.52 mmol/h and 5.19 mmol/h respectively; the fractional absorption rate of acetate and butyrate were 76.33% and 77.52% respectively. (2) the regression equation between the principal production rate (X, mmol/h)and the absorption rate of acetate and butyrate(Y, mmol/h) in the rumen of sheep were as follows: for acetate: Y=0.6905X+4.4554(R2=0.9973,n=5),for butyrate: Y=0.1514X+14.311(R2=0.8227,n=5);the regression equation between the production rate of acetate and butyrate(X, mmol/h) in the rumen of sheep and the rumen concentration of acetate and butyrate(Y, mmol/L) were as follows: for acetate: y = 0.3739x + 5.4456(R2 = 0.9899, n=5), for butyrate: y = 0.3617x + 1.8599(R2 = 0.9247,n=5.)(3)Dietary MLS and MG value were 135.69g and 43.83g respectively; the ratio of MLS/MG was 3.10; the ratio of C2+C4 energy and of C3+C6 energy in dietary ME were 89.24%和11.30% respectively.
Exp2: The utilization of acetate and butyrate in the rumen in splanchnic tissues of the sheep
Three inner Mongolian semifine-wool wethers (1.5 old years, 29-31kg BW) fed 1.0 M basal diet and fitted with chronic indwelling catheters in the mesenteric vein, hepatic
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