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杂交茎瘤芥营养与施肥效应研究
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摘要
茎瘤芥(Brassica juncea var. tumida Tsen et Lee)是榨菜的原料植物,以“涪陵榨菜”为代表的榨菜产业是重庆市尤其是涪陵区农业、农村、农民的重要经济支柱。茎瘤芥生产上,长期以来存在偏施氮肥、磷肥用量不足、钾肥用量极低、氮磷钾养分施用不平衡等问题,导致施肥量逐年增大、肥料面源污染日趋严重,土壤生态系统恶化,茎瘤芥品质下降,投入产出比呈递减趋势。
     随茎瘤芥杂交种的问世,推动了榨菜产业的发展,但与杂交种配套的栽培技术,尤其是施肥对杂交茎瘤芥的影响,缺少研究。
     本文针上述问题,采用田间试验和室内分析相结合的方法,研究了杂交茎瘤芥物质积累规律、氮磷钾吸收规律、不同施钾量和施磷量对杂交茎瘤芥产量和品质的影响,不同施肥水平对土壤脲酶活性的影响,目的在于探明杂交茎瘤芥营养规律及施肥对其产量和品质效应,了解不同施肥水平下,对土壤脲酶活性的影响,为杂交茎瘤芥丰产、优质施肥提供理论依据。
     主要研究结果如下:
     1、杂交茎瘤芥物质积累规律
     茎瘤芥杂交种与常规种一样,各器官物质积累规律均符合Logistic生长曲线。根在14.9叶龄物质积累速度最大、茎在17.8叶龄、叶在14.7叶龄。根旺盛生长期在12.7—17.0叶龄,持续4.3叶龄,此期积累66.4%的干物质;叶旺盛生长期在12.6—16.8叶龄,持续4.2叶龄,此期积累67.2%的干物质。根和叶的旺盛生长期基本一致,在旺盛生长期积累的物质量在整个营养生长阶段所占的比例也较为接近。
     茎旺盛生长期在15.3—20.2叶龄,持续4.9叶龄,此期积累85.4%的干物质;茎的旺盛生长期出现晚2.6—2.7个叶龄单位,但持续的时间却较根和叶长0.6-0.7个叶龄单位,旺盛生长期内物质积累占整个营养生长阶段的比例也较根和叶高18.2—19.0%。
     2、杂交茎瘤芥氮磷钾营养规律
     杂交茎瘤芥对氮、磷、钾的吸收是一个先慢后快,再逐渐减慢的过程。在12叶以前,氮、磷、钾的吸收量较小,氮36%、磷14%、钾16%左右;在13~18叶,各养分吸收量较大,氮59%,磷70%、钾73%左右;在后期对3种肥料的吸收量较小。杂交茎瘤芥对磷、钾的旺盛吸收期基本上在13~18叶龄,氮肥的旺盛吸收期偏早,基本上在11—16叶龄。旺盛吸收期对氮、磷、钾的吸收量占大田]营养生长阶段吸收总量的比例均较高,氮占68.9%、磷70.3%、钾72.7%,各肥料在旺盛吸收期吸收速率和吸收量有较大差异。
     生产上,栽种667m2的杂交茎瘤芥,目标产量若定为3500kg,需消耗纯氮33.56kg、磷(P2O5)7.63kg、钾(K2O)25.47kg,其比例为1:0.23:0.76。在生产实际中,施肥量的确定还需根据土壤养分含量情况,进行相关计算。
     3、钾肥对茎瘤芥产量和品质影响
     在当前施肥水平下,增施钾肥能显著提高瘤茎产量,施钾量为12 kg/667m2时,产量最高,超过此水平,不再显著提高。
     施钾在一定程度上能降低菜皮含量,改善瘤茎菜形指数,提高加工品质。提高瘤茎重要加工品质的施钾量为12.74kg/667m2。percentage
     施钾与茎瘤芥瘤茎营养品质呈显著正相关,施钾能快速提升可溶性糖含量、粗蛋白含量、Vc的含量。提高瘤茎重要营养品质的施钾量为12.67kg/667m2。
     适当增施钾肥,可降低硝酸盐含量,但过量施用钾肥则相反,当施钾量在12 kg/667m2时,硝酸盐约为1333mg/kg。
     本研究认为,在灰棕紫泥土壤杂交茎瘤芥生产实际中,氮、磷施用相同的基础上,综合考虑产量和品质,施钾量(K2O)以12kg/667m2为最佳。
     4、磷肥对茎瘤芥产量和品质影响
     茎瘤芥栽培上施磷具有较好的增产效应,但高施磷量(>7.8 kg/667m2),将促进叶的生长而不利于瘤茎生长。
     各营养品质中,可溶性糖含量与磷肥的呈正相关,低施磷量下粗蛋白质含量较高,高施磷量下粗蛋白质含量将大幅下降。在低于8.36 kg/667m2内,随施磷量的增加,Vc含量上升,即当施磷量超过8.36 kg/667m2后VC含量将下降。适当增施磷肥可降低硝酸盐含量,但过量施用磷肥则相反。
     本研究认为,在灰棕紫泥土壤杂交茎瘤芥生产实际中,氮、钾施用相同的基础上,综合考虑产量和品质,推荐施磷量(P2O5)6.5 kg/667m2较能保障各品质。
     5、施肥对茎瘤芥土壤脲酶活性的影响
     本研究发现,施肥均会提高土壤脲酶活性,一般而论,除快速膨大期外,施肥水平越高,脲酶活性越强。茎瘤芥快速膨大期,高施肥处理土壤脲酶活性低于中施肥处理。快速膨大期到采收期脲酶活性的增量与瘤茎产量呈显著正相关。
Stem mustard is the material plants of preserved szechuan pickle, which is the important economic pillar industry of agriculture,country and former in Chongqing city, especially in Fuling area.Some problem in stem mustard production such as partial Long-term Nitrogen, lack of phosphate fertilizer , less of potassium fertilizer, imbalance of NPK nutrient, which lead to fertilizer rate ascend,fertilizer Non-point source Pollution worsen,soil eco-system deteriorated, stem mustard quality declined andinput-output ratio descended.
     Hybrid Tumour stem mustard promote the develop of preserved szechuan pickle industry, the research on cultivati Techniques matching of hybird seed is scarcitied,especial the effect of fertilizer on hybird Stem mustard.
     In this study, field experiment and laboratory analysis were combined to study for the law of stem mustard hybrid matter accumulation , absorption regularity N,P,K, the effect on yield and quality of Stem Mustard Hybrid by different potassium and phosphor ,and the effect on soil urease activity by different fertilization levels. The objectives were as follows: prove up the Nutrition law of stem mustard hybrid,the effect of yield and quality by fertilization,find out the effect on oil urease activity by different fertilization levels, in order to provide theoretical basis for high yield and high effective fertilization of stem mustard hybrid.
     1、The law of stem mustard hybrid matter Accumulation
     The law of the various apparatustem matter accumulation conform to the logistic growth curve in mustard hybrid matter, the same as conventional cultivar. The accumulating rate reached the maximum value at 14.9 leaf age for root,17.8 leaf age for stem, 14.7 leaf age for leaf.
     Root reached the fast growth period in 12.7—17.0 leaf age and last for 4.3 leaf age,66.4%of dry matter were accumulated;leaf reached fast growth period in 12.6—16.8 leaf age and last for 4.2 leaf age,67.2% of dry matter were accumulated. The fast growth period of root was consisted with leaf,and the proportion of accumulation in fast growth period on vegetative growth phase in root was approach to leaf.
     Stem reached the fast growth period in 15.3—20.2 leaf age and last for 4.9leaf age,85.4% of dry matter were accumulated; the fast growth period of stem was lated 2.6-2.7 leaf age than root and leaf,but the lasting time was longer than then about 0.6-0.7 leaf age.The proportion of accumulation in fast growth period on vegetative growth phase was higher than root and leaf about 18.2—19.0%.
     2、The nutrition law of stem mustard hybrid to N,P,K
     The absorption process of stem mustard hybrid to N,P,K was form slow to fast,and to slow. Absorbing capacity of N,P,K were low before 12 leaf age, which only accounted for 36%,14%,and 16%;absorbing capacity of N,P,K were higher in 13-18 leaf age,about 59%,70% and 73%; absorbing capacity of three elements were lower in late stage.The vigorous absorption period of stem mustard hybrid to P,K is in 13-18 leaf age,later than N ,which is in 11-16 leaf age. The proportion of absorping capacity of N,P,K in vigorous absorption period in field vegetative growth phase was higher,about 68.9%,70.3%,727.7%.The absorption of three element was different in absorption velocity and absorption capacity in vigorous absorption period.
     was 3500kg,the consumption of pure nitrogen, P2O5, K2O was 33.56kg,7.63kg,25.47kg,with ratio of 1:0.23:0.76.
     In actual production,the fertilizer rate need to be determined by soil nutrient and correlation calculation.
     3、The effect of Potash on yield and quality of stem tumorous mustard
     In the current fertilization level, applying potash fertilizer can increased the yield of tumorous stem significantly .The highest yield was 12 kg/667m~2 for Potash, but will not increased significantly when over the limit.
     Applying potash fertilizer could reduce the content of fur of vegetable, improve tumorous stem index and processing quality. Potash fertilizer could impove the tumorous stem processing quality for 12.74kg/667m~2.
     Potash fertilizer had significant positive correlation with nutritional quality of tumorous stem, Potash fertilizer could impove the content of soluble sugar, crude protein and Vc. Potash fertilizer impove the tumorous stem processing quality for 12.67kg/667m~2.
     With applying Potash fertilizer reasonably could reduce the content of nitrate,excessive Potash did negative effects.When Potash was 12 kg/667m~2, nitrate was 1333mg/kg.
     The results showed that, during the stem mustard hybrid practical production in grey brown purple soil, based on the same N and P fertilizer application, on the consideration of the effects of yield and quality, the properly potash rate was 12kg/667m~2 .
     4、The effects of phosphate on yield and quality of stem tumorous mustard
     With phosphate application, yield increasing effect of stem tumorous mustard would better,but high phosphate rate (7.8 kg/667m2)would promote the leaf growth and go against to tumorous mustard.
     In various nutritional quality, the contents of soluble sugar positively correlated with phosphate rate, the contents of crude protein decreased under high level phosphate rate.Lower than 8.36 kg/667m2, Vc content would increase when phosphate fertilizer increaed,and decreased when phosphorus rate beyond 8.36 kg/667m2.
     Properly application of phosphate fertilizer could decrease nitrate content and go against to excessive application.
     The results showed that, during the stem mustard hybrid practical production in grey brown purple soil, based on the same N and P rate application, the properly phosphate rate(P2O5)was 6.5 kg/667m2.
     5、The effect of fertilization on soil urease activity in tumorous stem mustard
     The results showed that, fertilization would raise the soil urease activity.
     In general,except fast swelling phase,the higher fertilization level was, the higher soil urease activity was.In fast swelling phase of tumorous stem mustard, soil urease activity in high-fertilizer was lower than in moderate-amount fertilizer. The incremental soil urease activity had a significant positive correlation with the yield of the tumorous stem between fast swelling phase to collection period.
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