桃仁质量研究
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摘要
桃仁来源于蔷薇科(Rosaceae)植物桃Prunus persica(L-)Batsch.或山桃P.davidiana(Carr.)Franch.的干燥成熟种子。其功效为活血祛瘀,润肠通便。用于经闭,痛经,癥瘕痞块,跌扑损伤,肠燥便秘。为活血化瘀要药,临床功效卓著,在血府逐瘀汤(《医林改错》)、生化汤(《傅青主女科》)、桂枝茯苓丸(《金匮要略》)、桃核承气汤(《伤寒论》)等医家名方中均重用桃仁,现代研究亦表明桃仁对心脑血管疾病疗效显著,近年来受到广泛关注。
本课题通过本草考证及现代商品调查研究,明确了桃仁的用药历史,及其历代药用概况,为追踪桃仁的功效物质基础提供了文献支撑,收集了2005年版《中国药典》收录的桃仁两个基源品种蔷薇科(Rosaceae)植物桃P.persica(L.)Batsch.或山桃P.davidiana(Carr.)Franch.的34个不同品种和不同产地的桃仁样品。总结了桃仁的分布概况、主产地、及商品流通地。
通过对桃仁化学成分的系统研究,明确了桃仁中的化学成分类型,以桃仁历代本草记载及临床应用的活血化瘀功效为研究目标,采用药理学方法,筛选出了能代表桃仁内在质量并与其功能主治活血化瘀基本吻合的有效部位——石油醚提取部位,在此基础上运用现代先进提取、分离、纯化技术及手段,从石油醚部分得到了3个含量较高化合物,用波谱解析、化学分析的方法鉴定其结构,分别是:棕榈酸、油酸、亚油酸。首次通过小鼠凝血时间的测定、小鼠耳廓微循环、大鼠寒凝血瘀证三个活血化瘀药效验证实验,证明桃仁油(亚油酸、油酸的相对含量＞90%)有显著的活血化瘀作用,确定了其中的不饱和脂肪酸亚油酸、油酸为桃仁活血化瘀有效组分之一。
首次采用气相色谱与质谱(GC-MS)联用技术,对桃仁活血化瘀有效部位——脂肪酸部位进行了分析研究,建立了桃仁药材34个样品脂肪酸GC-MS指纹图谱及采用GC-MS评价桃仁的方法,为对桃仁活血化瘀的物质基础进行控制,以及桃仁在临床上的深入应用和桃仁在制剂中的深开发提供了理论基础和客观指标。
采用气相色谱(GC)法测定了34个桃仁样品中的棕榈酸、油酸、亚油酸的含量,同时采用高效液相色谱(HPLC)法测定了34个桃仁样品中的苦杏仁苷的含量,建立了采用HPLC和GC评价桃仁的方法,并提出了标准。初次建立了基于桃仁有效组分、主要毒副作用成分苦杏仁苷的科学、先进、操作性强的多指标质量评价体系,制定能够确切反映桃仁内在质量的量化评价指标,并起草了桃仁的质量标准,争取成为国家参照执行的药材标准,为新版《中国药典》桃仁质量提供新标准,为其临床应用提供理论基础。
通过对桃仁品种、品质与药效相关性的分析,首次建立了桃仁品质与药效相关性的数学模型,该模型较好的反应与印证了前面章节所做研究和分析的结果。如桃仁的品种(桃仁、山桃仁)和产地(主产地、非主产地)都与桃仁的品质具有较好的相关性,该模型对桃仁的药效评价具有很好的参考价值,这对桃仁的药效筛选有一定的指导意义。
Semen persicae for Blood Circulation to drugs,clinical their effectiveness,and from Rosaceae(Rosaceae) plant peach Prunus persica(L.) Batsch.Prunus davidiana or Prunus davidiana(Carr.) Franch.Dry mature seeds.Huoxue Quyu for their effectiveness,Runchang purge.For the closure,dysmenorrhea,with Zheng Jia Seoul block or flutter injury,intestinaldry constipation.Xuefuzhuyu in soup("YiLin Gaicu"),biochemicalsoup("Section Fuqingzhunvke woman"),Guizhifuling Pill ("JinKuiYaoLue"),Taohe Dachengqitang("Typhoid" ) Doctors who are arguably the reuse Semen persicae,modern research also shows that Semen persicae on cardiovascular and cerebrovascular diseases notable effect,received extensive attention in recent years.
Textual research this issue through research and modern goods,defined Semen persicae medication history,and ancient medicinal Overview,Below Semen persicae tracking the effectiveness of the base to provide material support literature,the 2005 edition of the collection "Chinese Pharmacopoeia" recorded two Semen persicae a variety of sources Rosaceae(Rosaceae) plant peach Prunus persica(L.) Batsch.or Prunus davidiana(Carr.) Franch.in 34 different varieties and different origin Semen persicaesamples.Familiar with the profile of peach kernel,the main origin,and the circulation of commodities.
The topic of Semen persicae chemical constituents through systematic study on the Semen persicae clear in the chemical composition of type,combining ancient herbal medicine Semen persicae Blood Circulation documented effectiveness of pharmacology at the same time,selected to represent Semen persicae inherent quality, and its function Indications -- Blood Circulation effective basic anastomosis site proved Semen persicae Blood Circulation for the material basis of the petroleum ether extract Semen persicae site and,based on this use of modern advanced extraction, separation,purification techniques and means,that is,use of modern advanced separation Semen persicae means of the chemical composition of petroleum ether site study from the petroleum ether have been part of three high content of compounds, using spectral analysis,chemical analysis of the identified structure,namely:palmitic acid,oleic acid,linoleic acid.The first time the clotting time of mice,mouse ear microcirculation,Hanning rat blood stasis three Blood Circulation.pharmacodynamics verification test to prove that palm oil acid,linoleic acid,unsaturated fatty acids for two Huoxue of Semen persicae Stasis effective component.
First used gas chromatography and mass spectrometry(GC-MS) was used for the peach kernel fatty acid sites were analyzed studies,the establishment of the 34 samples Semen persicae medicine fatty acids by GC-MS fingerprinting for Semen persicae Blood Circulation basis of the material control,and peach kernel in a more in-depth clinical applications and peach kernel in the preparation of the application and provides the theoretical basis of objective indicators.
First used gas chromatography(GC) Determination of the 34 samples of Semen persicae palmitic acid,oleic acid,linoleic acid content,using high performance liquid chromatography(HPLC) determination of the 34 samples of the plight Semen persicae Almond by HPLC,peach kernel based on the initial establishment of an effective component,the main side effects ingredients amygdalin science,advanced, easy-to the Multiple Indicator quality evaluation system developed to accurately reflect the inherent quality of Semen persicae quantified appraisal,and drafted Semen persicae quality standards,a national reference for the implementation of the standard medicines for the new version of "Chinese Pharmacopoeia" Semen persicae provide a new quality standard for its clinical application to provide'a theoretical basis.
Through the peach seed varieties,the quality and relevance of the effect of the establishment of a peach kernel quality and relevance of the effect mathematical model,the model better response and confirmed the previous chapters by the results of research and analysis.If Semen persicae species(Semen persicae mountain peach kernel),and origin(the origin,the non-origin) are and the quality is better Semen persicae the relevance of the peach kernel of the model has a good evaluation of the efficacy of the reference value,which the Semen persicae Screening effect to a certain extent guiding significance.
本课题通过本草考证及现代商品调查研究,明确了桃仁的用药历史,及其历代药用概况,为追踪桃仁的功效物质基础提供了文献支撑,收集了2005年版《中国药典》收录的桃仁两个基源品种蔷薇科(Rosaceae)植物桃P.persica(L.)Batsch.或山桃P.davidiana(Carr.)Franch.的34个不同品种和不同产地的桃仁样品。总结了桃仁的分布概况、主产地、及商品流通地。
通过对桃仁化学成分的系统研究,明确了桃仁中的化学成分类型,以桃仁历代本草记载及临床应用的活血化瘀功效为研究目标,采用药理学方法,筛选出了能代表桃仁内在质量并与其功能主治活血化瘀基本吻合的有效部位——石油醚提取部位,在此基础上运用现代先进提取、分离、纯化技术及手段,从石油醚部分得到了3个含量较高化合物,用波谱解析、化学分析的方法鉴定其结构,分别是:棕榈酸、油酸、亚油酸。首次通过小鼠凝血时间的测定、小鼠耳廓微循环、大鼠寒凝血瘀证三个活血化瘀药效验证实验,证明桃仁油(亚油酸、油酸的相对含量＞90%)有显著的活血化瘀作用,确定了其中的不饱和脂肪酸亚油酸、油酸为桃仁活血化瘀有效组分之一。
首次采用气相色谱与质谱(GC-MS)联用技术,对桃仁活血化瘀有效部位——脂肪酸部位进行了分析研究,建立了桃仁药材34个样品脂肪酸GC-MS指纹图谱及采用GC-MS评价桃仁的方法,为对桃仁活血化瘀的物质基础进行控制,以及桃仁在临床上的深入应用和桃仁在制剂中的深开发提供了理论基础和客观指标。
采用气相色谱(GC)法测定了34个桃仁样品中的棕榈酸、油酸、亚油酸的含量,同时采用高效液相色谱(HPLC)法测定了34个桃仁样品中的苦杏仁苷的含量,建立了采用HPLC和GC评价桃仁的方法,并提出了标准。初次建立了基于桃仁有效组分、主要毒副作用成分苦杏仁苷的科学、先进、操作性强的多指标质量评价体系,制定能够确切反映桃仁内在质量的量化评价指标,并起草了桃仁的质量标准,争取成为国家参照执行的药材标准,为新版《中国药典》桃仁质量提供新标准,为其临床应用提供理论基础。
通过对桃仁品种、品质与药效相关性的分析,首次建立了桃仁品质与药效相关性的数学模型,该模型较好的反应与印证了前面章节所做研究和分析的结果。如桃仁的品种(桃仁、山桃仁)和产地(主产地、非主产地)都与桃仁的品质具有较好的相关性,该模型对桃仁的药效评价具有很好的参考价值,这对桃仁的药效筛选有一定的指导意义。
Semen persicae for Blood Circulation to drugs,clinical their effectiveness,and from Rosaceae(Rosaceae) plant peach Prunus persica(L.) Batsch.Prunus davidiana or Prunus davidiana(Carr.) Franch.Dry mature seeds.Huoxue Quyu for their effectiveness,Runchang purge.For the closure,dysmenorrhea,with Zheng Jia Seoul block or flutter injury,intestinaldry constipation.Xuefuzhuyu in soup("YiLin Gaicu"),biochemicalsoup("Section Fuqingzhunvke woman"),Guizhifuling Pill ("JinKuiYaoLue"),Taohe Dachengqitang("Typhoid" ) Doctors who are arguably the reuse Semen persicae,modern research also shows that Semen persicae on cardiovascular and cerebrovascular diseases notable effect,received extensive attention in recent years.
Textual research this issue through research and modern goods,defined Semen persicae medication history,and ancient medicinal Overview,Below Semen persicae tracking the effectiveness of the base to provide material support literature,the 2005 edition of the collection "Chinese Pharmacopoeia" recorded two Semen persicae a variety of sources Rosaceae(Rosaceae) plant peach Prunus persica(L.) Batsch.or Prunus davidiana(Carr.) Franch.in 34 different varieties and different origin Semen persicaesamples.Familiar with the profile of peach kernel,the main origin,and the circulation of commodities.
The topic of Semen persicae chemical constituents through systematic study on the Semen persicae clear in the chemical composition of type,combining ancient herbal medicine Semen persicae Blood Circulation documented effectiveness of pharmacology at the same time,selected to represent Semen persicae inherent quality, and its function Indications -- Blood Circulation effective basic anastomosis site proved Semen persicae Blood Circulation for the material basis of the petroleum ether extract Semen persicae site and,based on this use of modern advanced extraction, separation,purification techniques and means,that is,use of modern advanced separation Semen persicae means of the chemical composition of petroleum ether site study from the petroleum ether have been part of three high content of compounds, using spectral analysis,chemical analysis of the identified structure,namely:palmitic acid,oleic acid,linoleic acid.The first time the clotting time of mice,mouse ear microcirculation,Hanning rat blood stasis three Blood Circulation.pharmacodynamics verification test to prove that palm oil acid,linoleic acid,unsaturated fatty acids for two Huoxue of Semen persicae Stasis effective component.
First used gas chromatography and mass spectrometry(GC-MS) was used for the peach kernel fatty acid sites were analyzed studies,the establishment of the 34 samples Semen persicae medicine fatty acids by GC-MS fingerprinting for Semen persicae Blood Circulation basis of the material control,and peach kernel in a more in-depth clinical applications and peach kernel in the preparation of the application and provides the theoretical basis of objective indicators.
First used gas chromatography(GC) Determination of the 34 samples of Semen persicae palmitic acid,oleic acid,linoleic acid content,using high performance liquid chromatography(HPLC) determination of the 34 samples of the plight Semen persicae Almond by HPLC,peach kernel based on the initial establishment of an effective component,the main side effects ingredients amygdalin science,advanced, easy-to the Multiple Indicator quality evaluation system developed to accurately reflect the inherent quality of Semen persicae quantified appraisal,and drafted Semen persicae quality standards,a national reference for the implementation of the standard medicines for the new version of "Chinese Pharmacopoeia" Semen persicae provide a new quality standard for its clinical application to provide'a theoretical basis.
Through the peach seed varieties,the quality and relevance of the effect of the establishment of a peach kernel quality and relevance of the effect mathematical model,the model better response and confirmed the previous chapters by the results of research and analysis.If Semen persicae species(Semen persicae mountain peach kernel),and origin(the origin,the non-origin) are and the quality is better Semen persicae the relevance of the peach kernel of the model has a good evaluation of the efficacy of the reference value,which the Semen persicae Screening effect to a certain extent guiding significance.
引文
[1]中国药典委员会.中华人民共和国药典(一部)(2005年版)[M].北京:化学工业出版社,2005:196
[2]罗国安,王义明,曹进,等.建立我国现代中药质量标准体系的研究.世界科学技术-中药现代化,2002 4(4):5.
[3]李晓光,陈志霞.中药质量标准研究方法与思路.山东中医药大学学报,2001 25(3):235-237.
[4]罗国安,王义明.中药材的质量标准.中药研究与信息,1999(6):21-23.
[5]罗国安,王义明,饶毅.中药中成药现代化进展.中成药,2000,22(1)71-79.
[6]罗国安,王义明.中药复方的化学研究体系.世界科学技术-中药现代化,1999(1):16-19.
[7]徐亚静.中药复方药效物质基础研究重点.中国医药报,2003 10,30.
[8]罗国安,王义明,饶毅.中药中成药现代化进展.中成药,2000 20(1):71.
[9]张南平,肖新月,张萍.建立中药“对照指纹图谱”的可行性探讨.中国药事,2003(17)6:347-350,
[10]NatalieLazarowyeh.UseofFingerprintingandMakerCompoundsforIndentifieationandSandardi zatinofBotanieal(J).DrugInformationJournal,1998,32:497- 512.
[11]PeterJoneHoughton.EstablishingIdentifieationCriteriaforBotanieals (J).DruglnformationJournal,1998,32:461- 469.
[12]FDAGuidanceforlndustry- BotaniealDrugProduets(DraftGuidanee),Ⅷ,B,Ze:3e:August 2000.
[13]BrankaBarl.QualityAnalysisandStandardizationExtraetsofMedieinalHerbs (M).PMAP1997eonfereneefrooInternet.
[14]WHO Guidenees For the Assessment of Herbal Medieines,1996 Genev.
[15]RudolfBauer.QualityCriteriaandPhytopharmaeeutieals:CanAeeeptableDrugStandardsbeaehie ved?[J].DrugInforationJournal,1998,32:101- 110.
[16]谢培山.中药制剂色谱指纹图谱(图象)鉴别.中成药,2000,22(6):390-395.
[17]苏颂撰.胡乃长,王致谱辑.图经本草.福建科学技术出版社,1988:493
[18]吴普等述.孙星衍,孙冯翼辑.神农本草经.上海:科学技术文献出版社,1996:118
[19]陶弘景.名医别录(辑校本).北京:人民卫生出版社,1986:306
[20]朱丹溪,撰.陶广正,刘玉玮,点校.本草衍义补遗.见:天津科学技术出版社总纂.金元四大家医学全书.天津:天津科学技术出版社,1994:1022
[21]缪希奎,撰.本草经疏.见:马子密,傅延龄,主编.历代本草药性汇解.北京:中国医药科技出版社,2002:540
[22]刘国强.桃仁承气汤治疗慢性肾盂肾炎46例.吉林中医药,1986,(4):10
[23]田浴尘.桃仁汤治疗妊娠高血压病27例.山东中医学院学报,1978,(增刊):19
[24]严淑珍.中药外敷法治疗小儿疮积.河北中医,1991,(4):10
[25]张廷模.中药学[M].高等教育出版社,2002.265.
[26]原田正敏.汉方生药制剂品质评价[M].东京:广川书店株式会社,1992:76.
[27]袁丹,胡爽等.桃仁药材质量评价法的研究[J].中国药学杂志,2003,3(1):53
[28]徐国钧,徐珞珊.常用中药材品种整理和质量研究.福建科学出版社,1997:832
[29]王旭.苦杏仁混充桃仁的原因及解决办法的探讨.基层中药杂志,2001,15(3):38
[30]森重英明等.Studies on the Constituents of Persicae Semen(Ⅰ)生药学杂志,1983,37(1):46
[31]有地滋等.汉药桃仁的研究.国外医学,中医中药分册,1983,5(3):50
[32]蔡双莲,李敏.多不饱和脂肪酸的研究进展.生命科学研究,2003,7(4):289-304
[33]周兴,林成仁,饶琳,等.n-3多烯脂肪酸对血脂影响的实验研究[J].中国中医基础医学杂志,1997,3(4):29
[34]张清波等 用激光多普耳血流量仪及胆汁流量计测定桃仁提取物对肝脏微循环的影响.上海中医药杂志,1985,(7):45
[35]朱萱萱等.桃仁、防已提取物对大鼠血小板聚集作用的研究.中医药研究,2000,16(3):45
[36]Zheng JX..Functionality Eatables(功能食品)[M].Beijing.China Light Industry Press.1995
[37]Takou Kosuge,Hitoshi lshida,Hiochiro Yamazaki,etnl.Study on active sub stances in the herbs used for oket-SU:Blood coagulation,in Chinese med icine on anticoagu-lative activitical of the herbs used for oketsu[J].Journal of the Pharmaocutical Society of Japan,1984,104(10):1050.
[38]韩婷,宓鹤鸣等.姜黄的化学成分及药理活性研究进展[J].解放军药学学报,2001,17(2):95
[39]原田正敏.汉方生药制剂品质评价[M].东京:广川书店株式会社,1992:76.
[40]袁丹,胡爽等.桃仁药材质量评价法的研究[J].中国药学杂志,2003,3(1):53
[41]崔玉荣,卢涛.以油角为原料制取精油酸[J].郑州轻工业学院学报,1992,7(2):29-34
[42]傅红,裘爱咏.分子蒸馏法制备鱼油多不饱和脂肪酸[J].无锡轻工大学学报,2002,21(6):617-621
[43]杨村,于宏奇,冯武文.分子蒸馏技术[M].北京:科学出版社,2003:79-87
[44]王明霞,黄凤洪.多不饱和脂肪酸萃取中超临界技术的应用进展[J].中国油脂,2005,30(8):60-64
[45]Michihata T,Matsud Y,Yasuo S,et al.Extraction of lipid from the reside of ika-ishiru using supercritical cbom dioxide[J].Journal of Japanese society offood science,1997,44(11):795-800
[46]陈永,赵辉,江鸿等.混合脂肪酸的分离 化学世界,2000,6(3):156-159
[47]黄泰康.常用中药成分与药理手册.下册[M].北京:中国医药科技出版社,1999:1491
[48]文川,徐浩,黄启福,等.活血中药对ApoE基因缺陷小鼠血脂及动脉粥样硬化斑块炎症反应的影响[J].中国 中西医结合杂志,2005,25(4):345.
[49]Park wH,Hong MY,Chung KH,et al.Effects of traditional herbal medicine,Hwaotang,on atherosclerosis using the spontaneous familial hypercholes-terolemia model,Kurosawa and K usanagi-hypercholes-terolemic rabbits and the venous thrombosis rats[J].Phytother Res,2005,19(10):846.
[50]Park WH,Shin SS,Lee YC,et al.The inhibitory effects of Silso-San-Gami-onatherosclerosis in KHC rabbits[J].Thrornb Res,2004,113(3-4):235.
[51]朱萱萱,朱芳,施荣山.桃仁、防己提取物对大鼠血小板聚集作用的研究.中医药研究,2000,16(3):44
[52]赵国荣等.四逆散、逍遥散及其配伍丹参或桃仁对大鼠免疫性肝损伤影响的对比研究.湖南中医学院学报,1999,19(4):9
[53]徐列明等.桃仁提取物抗实验性肝纤维化的作用观察.中国中药杂志,1994,19(8):491
[54]孙维强等.桃仁抗肝脏脂质过氧化损坏作用的实验研究,湖南中医杂志,1993,9(6):178
[55]柴瑞雾等.桃仁“止咳“析议.上海中医药杂志,1991,(1):48
[56]邢国秀,李楠.天然苦杏仁苷的研究进展[J].中成药,2003,25(12):1007-1009
[57]李琴韵.HPLG法测定乳宁冲剂中苦杏仁苷的含量.中成药,1998,20(9):12-14
[58]Ma C,Li CH Determination of amygdalin in apricot,kernel[J]Chin J Exp Tradit M ed Form,2000,6(2):1 6-7.
[59]胡润淮.薄层扫描法测定麻黄汤冲剂中苦杏仁苷含量的研究[J].河南中医药学刊,1995,10(5):14-5
[60]赵索莲,等.苦杏仁苷对小鼠脾脏 细胞活性的影响.山西医学院学报,1993,(1):14
[61]赵素莲,等苦杏仁苷对小鼠免疫功能的影响 山西医药杂志,1993,(3):166
[62]李春华,等苦杏仁苷对小鼠肝枯否细胞DNA活性及吞噬功能的影响.实用中西医结合杂志,1994,(1):7
[63]张宝香,金春爱,赵延平.鹿角盘的化学成分与开发利用.[J]特种经济动植物.2005.(8)12:7
[64]李泽鸿,姚玉霞,王全凯等..二杠鹿茸和二杈鹿茸中营养元素含量的差异[J].微量元素和健康研究.2003.20(4):30-31
[65]罗国安,王义明.中药复方物质基础和药效相关性研究[J]世界科学技术-中药现代化,1999,1(1):11.
[66]甘师俊,李振吉,邹健强.中药现代化发展战略[M]北京:科学技术文献出版社,1998
[67]谢培山.中药制剂色谱指纹图谱(图像)鉴别[J]中成药,2001,22(6):391
[1]吴普等述.孙星衍,孙冯翼辑.神农本草经.上海:科学技术文献出版社,1996:118
[2]陶弘景.名医别录(辑校本).北京:人民卫生出版社,1986306
[3]朱丹溪,撰.陶广正,刘玉玮,点校.本草衍义补遗.见:天津科学技术出版社总纂.金元四大家医学全书.天津:天津科学技术出版社,1994:1022
[4]缪希塑,撰.本草经疏.见:马子密,傅延龄,主编.历代本草药性汇解.北京:中国医药科技出版社,2002:540
[5]刘国强.桃仁承气汤治疗慢性肾盂肾炎46例.吉林中医药,1986,(4):10
[6]田浴尘.桃仁汤治疗妊娠高血压病27例.山东中医学院学报,1978,(增刊):19
[7]严淑珍.中药外敷法治疗小儿疮积.河北中医,1991,(4):10
[8]国家药典委员会 中华人民共和国药典一部.2005版.北京:北京化学工业出版社,2005:196
[9]江苏新医学院.中药大辞典.下册 上海:上海人民出版社。1977:1787
[10]明李时珍《本草纲目》校点本第三册.北京:人民卫生出版社,1978:1741
[11]徐国钧,徐珞珊.常用中药材品种整理和质量研究.福建科学出版社,1997.832
[12]王旭.苦杏仁混充桃仁的原因及解决办法的探讨.基层中药杂志,2001,15(3):38
[13]有地滋等.第四回天然药物0)开发七应用,夕术犷少A要旨集 1982:7
[14]庄司顺三.相七仁的化学,药理和生物化学 国外医学·中医中药分册,1987,9(4):31
[15]森重英明等.Studies on the Constituents of Persicae Semen(Ⅰ)生药学杂志,1983,37(1):46
[16]有地滋等.汉药桃仁的研究.国外医学,中医中药分册,1983,5(3):50
[17]苗和恺等.桃仁精油的化学成分研究 中成药,1992,14(2):33
[18]Choi Jae Sue et al.Antihyperhpide nic Effect of Flauonoids from Prunesdavidiana.J.Nat.Prod,1991,54(1):218
[19]国家中医药管理局《中华本草》编委会.中华本草.四册.上海:上海科技出版社,1999.75
[20]文川,徐浩,黄启福,等.活血中药对ApoE基因缺陷小鼠血脂及动脉粥样硬化斑块炎症反应的影响[J].中国 中西医结合杂志,2005,25(4):345.
[21]Park wH,Hong MY,Chung KH,et al.Effects of traditional herbal medicine,Hwaotang,on atherosclerosis using the spontaneous familial hypercholes-terolemia model,Kurosawa and K usanagi-hypercholes-terolemic rabbits and the venous thrombosis rats[J].Phytother Res,2005,19(10):846.
[22]Park WH,Shin SS,Lee YC,et al.The inhibitory effects of Silso-San-Gami-onatherosclerosis in KHC rabbits[J],Thrornb Res,2004,113(3-4):235.
[23]朱萱萱,朱芳,施荣山.桃仁、防己提取物对大鼠血小板聚集作用的研究.中医药研究,2000,16(3):44
[24]张秋海等.桃仁的研究进展.实用中西医结合杂志,1993,6(3):163
[25]朱萱萱等.桃仁、防已提取物对大鼠血小板聚集作用的研究.中医药研究,2000,16(3):45
[26]张清波等 用激光多普耳血流量仪及胆汁流量计测定桃仁提取物对肝脏微循环的影响.上海中医药杂志,1985,(7):45
[27]赵国荣等.四逆散、逍遥散及其配伍丹参或桃仁对大鼠免疫性肝损伤影响的对比研究.湖南中医学院学报,1999.19(4):9
[28]徐列明等.桃仁提取物抗实验性肝纤维化的作用观察.中国中药杂志,1994,19(8):491
[29]孙维强等.桃仁抗肝脏脂质过氧化损坏作用的实验研究,湖南中医杂志,1993,9(6):47
[30]吕文海等.桃仁炮制品的初步药理研究.中药材,1994,17(3):29
[31]张秋海等.桃仁的研究进展.实用中西医结合杂志,1993,6(3):163
[32]刘英等.炒桃仁总蛋白对小鼠B细胞功能影响的实验研究.中医药学报,2001,29(2):55
[33]国家中医药管理局《中华本草》编委会.中华本草.四册.上海:上海科技出版社,1999.75
[34]雷载权,张廷模主编.中华临床中药学(下卷).北京:人民卫生出版社,1998.1116
[35]方文贤,宋崇顺,周立孝,医用中药药理学。北京,人民卫生出版社,1998,280
[36]Chang HK,Shin MS,Yang HY,et al.Amygdalin induces apoptosis through regulation of Bax and Bcl-2 B expressions in human DU145 and LNCaP prostate cancer cells[J].Biol Pharrfl Bull,2006,29(8):1 597.
[37]Park I-tl,Yoon SH,Han LS,et al.Amygdalin inhibits genes related to cell cycle in SNU -C4 human colon cancer cells[J].Worle J Gastroenterol,2005,11(33):5 156.
[38]Kwon HY,Hong SP,Hahn DH,et al.Apoptosis induction of Persicae Semen extract in human promyelocytic leukemia(HL-60)cells[J].Arch Pharm Res,2003,26(2):157.
[39].孙秀坤,许爱娥.7种中药乙醇提取物及补骨脂素对人黑素痛YUGEN8细胞酪氨酸酶的影响[J].中华皮肤科杂志,2006,39(6):328.
[40]潘晶,宋淑云,蔡可津等.谈生桃仁炮制后对临床疗效的影响.吉林中医药,1994,(5):34
[41]吴斌等.“831”注射液治疗缺血性中风临床及实验观察.中西医结合杂志,1991,11(12):738
[42]刘成等.桃仁提取物合虫草菌丝治疗肝炎后肝硬化的观察.中医杂志,1991,32(7):20
[43]柴瑞雾等.桃仁“止咳“析议.上海中医药杂志,1991,(1):48
[44]刘乾和等.56例慢性咽炎治疗小结.上海中医药杂志,1989,(1):40
[45]田浴尘.活血化瘀法临床应用及其实验研究的进展.山东中医学院学报,1978:17
[46]周仲瑛等 泻下通奈法治疗流行性出血热少尿期86例疗效观察.江苏中医杂志,1985,6(10):27
[47]吕文海,卜永春.桃仁炮制品的初步药理研究.中药材,1994,17(3):29
[48]方文贤,宋崇顺,周立孝,医用中药药理学。北京,人民卫生出版社,1998,280
[49]崔玉荣,卢涛.以油角为原料制取精油酸[J].郑州轻工业学院学报,1992,7(2):29-34
[50]傅红,裘爱咏.分子蒸馏法制备鱼油多不饱和脂肪酸[J].无锡轻工大学学报,2002,21(6):617-621
[51]杨村,于宏奇,冯武文.分子蒸馏技术[M].北京:科学出版社,2003:79-87
[52]王明霞,黄凤洪.多不饱和脂肪酸萃取中超临界技术的应用进展[J].中国油脂,2005,30(8):60-64
[53]Michihata T,Matsud Y,Yasuo S,et al.Extraction of lipid from the reside of ika-ishiru using supercritical c bom dioxide[J].Journal of Japanese society offood science,1997,44(11):795-800
[54]陈永,赵辉,江鸿,等.混合脂肪酸的分离 化学世界,2000,6(3):156-159
[55]侯镜德,骆小明,冯建跃,等.顺-15-二十四碳烯酸的分离制备方法:中国,96 122232.8[P].
[56]Douglas G.Hayes.Effect of temperature programming on the perfor-mance of urea inclusionc ompound-based free fatty acid fractionation[J].Journal of the American Oil Chemists Society,2006,83(3):253-259
[57]Robert W,John R S.Chain separation of monounsaturated fatty acid methyl estem by argentation thin layer chromatography[J].Journal of chromatography A,2001,905(1):251-257
[58]张氽,阚建全,陈宗道.硝酸银-硅胶纯化a-亚麻酸的研究[J]离子交换与吸附,2005,21(1):47-54
[59]Rezanka T.Two-dimensional separation of fatty acids by thin-layer chromatography on urea and silver nitrate silica gel plates[J].Journal of chromatography A.1996,727(Ⅰ):147-151
[60]周兴,林成仁,饶琳,等.n-3多烯脂肪酸对血脂影响的实验研究[J].中国中医基础医学杂志,1997,3(4):29
[61]徐静,李姜溶,王健敏.多烯脂肪酸胶丸治疗高脂血症疗效观察[J].Heilongjiang medicine and pharmacy,1998,21(4):65
[62]周兴,林成仁,饶琳,等.n-3多烯脂肪酸对血小板聚、血栓形成、6-keto-PGR_(??)/TXB2比值的影响[J].中药药理与临床,1997,13(1):27
[63]Zimmer L,Delpal S,Guiloteau D,et al.Chronic n-3 polyunsaturated fatty acid deficiency alters dopamine vesicle density in the rat fomtal cortes[J].Neurosci Lett,2000,284:25
[64]Moussa M,Le Boucher J,Garcia J,et al.In vivo effects of olive oil-based lipid emulsion on lymphocyte activation in rats[J].Clin Nutr,2000,19:49
[65]Chavali SR,Forse RA.Decreased production of interleukin-6 an d pros- taglandin E2associated with inhibition of delta-5 desaturation of ome- ga-6 fatty acids in mice fed safflower oil diets supplemented with 8esa-mol[J].Prostaglandins Leukotrienes Essential Fatty Acids,1999.61:347
[66]Arbogast.BW,Neuman n JK,Arbogast LY,et al.Transient loss of serllm protective activity following short-term stress:A possible bioc hemical link between stress and atherosclerosis[J].J Psychosom Res,1994,38:871
[67]Sudheera S,Leitch J,Garg M.N-3 Polyunsaturated fatty acid supplementation alters inositol phosphate metabolism and protein kinase C activity in adult porcin cardiacmyocytes.Journal of Nutritional Biochemistry,2001,(12):7-11
[68]Leifert W.R,Dorian C.L,Jahangiri A,al e.Dietary fish oil prevents asynchronous contractility and alters Ca'-' handling in adult rat cardiomyocytes.J Nutr Biochem,2001,12(6):365-376
[69]Rinaldi B,Di Pien'o P,ViteUi MR,D'Amico M,Berrino L' Rossi F,Filippelli A.Effects of docosahexaenoic acid on calcium pathway in adult rat cardiomyocytes.Life Sci,2002,71(9):993-1004
[70]蔡双莲,李敏.多不饱和脂肪酸的研究进展.生命科学研究,2003,7(4):289-304
[71]Hu FB,Bronner L,Willett W C,et al.Fish and omega-3 fatty acid intake and risk of coronary heart disease in women.JAMA,2002,287(14):1815-1821.
[72]Iso H,Rexrode KM,Stampfer MJ,et al.Intake of fish and omega-3 fatty acids and risk of stroke in women.JAMA,2001,285(3):304-312.
[73]A review of some herbal and related products conmmonly used in Cancer patients.Jouma]of the Amercian Dretetic Associatin,1997、(10)
[74]赵索莲.等苦杏仁苷埘小鼠脾脏￡细胞活性的影响.山西医学院学报,1993,(1):14
[75]赵素莲,等苦杏仁苷对小鼠免疫功能的影响 山西医药杂志,1993,(3):166
[76]李春华,等苦杏仁苷对小鼠肝枯否细胞DNA活性及吞噬功能的影响.实用中西医结合杂志,1994,(1):7
[77]Syrigos KN et al.In vitro cytoloucrty following spedific activation of amygdalin by beta-glucosidase conjugated to aBladder cancer-associated monoclonalantibody.In J Cancer,1998,78(6):712
[78]徐列明.等.扁桃苷对大鼠肝贮脂细胞增殖和产生胶原的影响.新消化病学杂志,1997,(2):84
[79]屈燧林,等汉防己甲素、川芎嗪和苦杏仁苷对人身成纤维细胞的影响.中华肾脏病杂志,2000,16(3):186
[80]Role of the gastrointestitud microflora in amygdalin-inducedcyarude toxicity.Biochemical Pharmacology,1980,29:301
[81]王本样.现代中药药理学.天津:天津科学技术出版社,1997:1005-1007
[82]李熙民,等.苦杏仁苷药物动力学及其毒理初步研究.新药与临床,1986,5(3):141
[83]Moertel.Charles G.et al.A Pharmacologic and ToxicolgicalS tudy of Amygalin.JAMA,245591
[84]American Cancer Society latetrle-The Offical View Un-proven Remedies.Sept 10,1996
[85]Spencer PS.et al Neuolog diseases associated with ofplant components with toxic potentia]Envier Res,1993,62:106
[86]张国明,等苦杏仁苷在土体内的药物动力学.中国药理学报,1986,7(5):460
[87]Copalan venkataknshnan. et al. Exolytic hydrolysis of toxicplant glucosides by gumea pigliver cytosolic p_ glucosidase J Biol Chem. 1992. 267: 14027
[88]strugala GJ, et al. Small—Intest inal trasfer mechanism ofprunasin, the primary metabolite of the cyanogenic glycoside amygdalin. Hum Exp Toxicol, 1995, 14(11): 895
[89]Hays William S. et al Expression of cytosolic B-glucosidasein guinea pig liver cells. Hepatology, 1998, 28(1): 156
[90]GlewRH, etal. The mammalian brosd-specificity B—glucosidase in exen A, ed. B—glucosidase:Biochemistry and Molecular Biology Washington DC Araereian Chemical society , 1993: 83
[2]罗国安,王义明,曹进,等.建立我国现代中药质量标准体系的研究.世界科学技术-中药现代化,2002 4(4):5.
[3]李晓光,陈志霞.中药质量标准研究方法与思路.山东中医药大学学报,2001 25(3):235-237.
[4]罗国安,王义明.中药材的质量标准.中药研究与信息,1999(6):21-23.
[5]罗国安,王义明,饶毅.中药中成药现代化进展.中成药,2000,22(1)71-79.
[6]罗国安,王义明.中药复方的化学研究体系.世界科学技术-中药现代化,1999(1):16-19.
[7]徐亚静.中药复方药效物质基础研究重点.中国医药报,2003 10,30.
[8]罗国安,王义明,饶毅.中药中成药现代化进展.中成药,2000 20(1):71.
[9]张南平,肖新月,张萍.建立中药“对照指纹图谱”的可行性探讨.中国药事,2003(17)6:347-350,
[10]NatalieLazarowyeh.UseofFingerprintingandMakerCompoundsforIndentifieationandSandardi zatinofBotanieal(J).DrugInformationJournal,1998,32:497- 512.
[11]PeterJoneHoughton.EstablishingIdentifieationCriteriaforBotanieals (J).DruglnformationJournal,1998,32:461- 469.
[12]FDAGuidanceforlndustry- BotaniealDrugProduets(DraftGuidanee),Ⅷ,B,Ze:3e:August 2000.
[13]BrankaBarl.QualityAnalysisandStandardizationExtraetsofMedieinalHerbs (M).PMAP1997eonfereneefrooInternet.
[14]WHO Guidenees For the Assessment of Herbal Medieines,1996 Genev.
[15]RudolfBauer.QualityCriteriaandPhytopharmaeeutieals:CanAeeeptableDrugStandardsbeaehie ved?[J].DrugInforationJournal,1998,32:101- 110.
[16]谢培山.中药制剂色谱指纹图谱(图象)鉴别.中成药,2000,22(6):390-395.
[17]苏颂撰.胡乃长,王致谱辑.图经本草.福建科学技术出版社,1988:493
[18]吴普等述.孙星衍,孙冯翼辑.神农本草经.上海:科学技术文献出版社,1996:118
[19]陶弘景.名医别录(辑校本).北京:人民卫生出版社,1986:306
[20]朱丹溪,撰.陶广正,刘玉玮,点校.本草衍义补遗.见:天津科学技术出版社总纂.金元四大家医学全书.天津:天津科学技术出版社,1994:1022
[21]缪希奎,撰.本草经疏.见:马子密,傅延龄,主编.历代本草药性汇解.北京:中国医药科技出版社,2002:540
[22]刘国强.桃仁承气汤治疗慢性肾盂肾炎46例.吉林中医药,1986,(4):10
[23]田浴尘.桃仁汤治疗妊娠高血压病27例.山东中医学院学报,1978,(增刊):19
[24]严淑珍.中药外敷法治疗小儿疮积.河北中医,1991,(4):10
[25]张廷模.中药学[M].高等教育出版社,2002.265.
[26]原田正敏.汉方生药制剂品质评价[M].东京:广川书店株式会社,1992:76.
[27]袁丹,胡爽等.桃仁药材质量评价法的研究[J].中国药学杂志,2003,3(1):53
[28]徐国钧,徐珞珊.常用中药材品种整理和质量研究.福建科学出版社,1997:832
[29]王旭.苦杏仁混充桃仁的原因及解决办法的探讨.基层中药杂志,2001,15(3):38
[30]森重英明等.Studies on the Constituents of Persicae Semen(Ⅰ)生药学杂志,1983,37(1):46
[31]有地滋等.汉药桃仁的研究.国外医学,中医中药分册,1983,5(3):50
[32]蔡双莲,李敏.多不饱和脂肪酸的研究进展.生命科学研究,2003,7(4):289-304
[33]周兴,林成仁,饶琳,等.n-3多烯脂肪酸对血脂影响的实验研究[J].中国中医基础医学杂志,1997,3(4):29
[34]张清波等 用激光多普耳血流量仪及胆汁流量计测定桃仁提取物对肝脏微循环的影响.上海中医药杂志,1985,(7):45
[35]朱萱萱等.桃仁、防已提取物对大鼠血小板聚集作用的研究.中医药研究,2000,16(3):45
[36]Zheng JX..Functionality Eatables(功能食品)[M].Beijing.China Light Industry Press.1995
[37]Takou Kosuge,Hitoshi lshida,Hiochiro Yamazaki,etnl.Study on active sub stances in the herbs used for oket-SU:Blood coagulation,in Chinese med icine on anticoagu-lative activitical of the herbs used for oketsu[J].Journal of the Pharmaocutical Society of Japan,1984,104(10):1050.
[38]韩婷,宓鹤鸣等.姜黄的化学成分及药理活性研究进展[J].解放军药学学报,2001,17(2):95
[39]原田正敏.汉方生药制剂品质评价[M].东京:广川书店株式会社,1992:76.
[40]袁丹,胡爽等.桃仁药材质量评价法的研究[J].中国药学杂志,2003,3(1):53
[41]崔玉荣,卢涛.以油角为原料制取精油酸[J].郑州轻工业学院学报,1992,7(2):29-34
[42]傅红,裘爱咏.分子蒸馏法制备鱼油多不饱和脂肪酸[J].无锡轻工大学学报,2002,21(6):617-621
[43]杨村,于宏奇,冯武文.分子蒸馏技术[M].北京:科学出版社,2003:79-87
[44]王明霞,黄凤洪.多不饱和脂肪酸萃取中超临界技术的应用进展[J].中国油脂,2005,30(8):60-64
[45]Michihata T,Matsud Y,Yasuo S,et al.Extraction of lipid from the reside of ika-ishiru using supercritical cbom dioxide[J].Journal of Japanese society offood science,1997,44(11):795-800
[46]陈永,赵辉,江鸿等.混合脂肪酸的分离 化学世界,2000,6(3):156-159
[47]黄泰康.常用中药成分与药理手册.下册[M].北京:中国医药科技出版社,1999:1491
[48]文川,徐浩,黄启福,等.活血中药对ApoE基因缺陷小鼠血脂及动脉粥样硬化斑块炎症反应的影响[J].中国 中西医结合杂志,2005,25(4):345.
[49]Park wH,Hong MY,Chung KH,et al.Effects of traditional herbal medicine,Hwaotang,on atherosclerosis using the spontaneous familial hypercholes-terolemia model,Kurosawa and K usanagi-hypercholes-terolemic rabbits and the venous thrombosis rats[J].Phytother Res,2005,19(10):846.
[50]Park WH,Shin SS,Lee YC,et al.The inhibitory effects of Silso-San-Gami-onatherosclerosis in KHC rabbits[J].Thrornb Res,2004,113(3-4):235.
[51]朱萱萱,朱芳,施荣山.桃仁、防己提取物对大鼠血小板聚集作用的研究.中医药研究,2000,16(3):44
[52]赵国荣等.四逆散、逍遥散及其配伍丹参或桃仁对大鼠免疫性肝损伤影响的对比研究.湖南中医学院学报,1999,19(4):9
[53]徐列明等.桃仁提取物抗实验性肝纤维化的作用观察.中国中药杂志,1994,19(8):491
[54]孙维强等.桃仁抗肝脏脂质过氧化损坏作用的实验研究,湖南中医杂志,1993,9(6):178
[55]柴瑞雾等.桃仁“止咳“析议.上海中医药杂志,1991,(1):48
[56]邢国秀,李楠.天然苦杏仁苷的研究进展[J].中成药,2003,25(12):1007-1009
[57]李琴韵.HPLG法测定乳宁冲剂中苦杏仁苷的含量.中成药,1998,20(9):12-14
[58]Ma C,Li CH Determination of amygdalin in apricot,kernel[J]Chin J Exp Tradit M ed Form,2000,6(2):1 6-7.
[59]胡润淮.薄层扫描法测定麻黄汤冲剂中苦杏仁苷含量的研究[J].河南中医药学刊,1995,10(5):14-5
[60]赵索莲,等.苦杏仁苷对小鼠脾脏 细胞活性的影响.山西医学院学报,1993,(1):14
[61]赵素莲,等苦杏仁苷对小鼠免疫功能的影响 山西医药杂志,1993,(3):166
[62]李春华,等苦杏仁苷对小鼠肝枯否细胞DNA活性及吞噬功能的影响.实用中西医结合杂志,1994,(1):7
[63]张宝香,金春爱,赵延平.鹿角盘的化学成分与开发利用.[J]特种经济动植物.2005.(8)12:7
[64]李泽鸿,姚玉霞,王全凯等..二杠鹿茸和二杈鹿茸中营养元素含量的差异[J].微量元素和健康研究.2003.20(4):30-31
[65]罗国安,王义明.中药复方物质基础和药效相关性研究[J]世界科学技术-中药现代化,1999,1(1):11.
[66]甘师俊,李振吉,邹健强.中药现代化发展战略[M]北京:科学技术文献出版社,1998
[67]谢培山.中药制剂色谱指纹图谱(图像)鉴别[J]中成药,2001,22(6):391
[1]吴普等述.孙星衍,孙冯翼辑.神农本草经.上海:科学技术文献出版社,1996:118
[2]陶弘景.名医别录(辑校本).北京:人民卫生出版社,1986306
[3]朱丹溪,撰.陶广正,刘玉玮,点校.本草衍义补遗.见:天津科学技术出版社总纂.金元四大家医学全书.天津:天津科学技术出版社,1994:1022
[4]缪希塑,撰.本草经疏.见:马子密,傅延龄,主编.历代本草药性汇解.北京:中国医药科技出版社,2002:540
[5]刘国强.桃仁承气汤治疗慢性肾盂肾炎46例.吉林中医药,1986,(4):10
[6]田浴尘.桃仁汤治疗妊娠高血压病27例.山东中医学院学报,1978,(增刊):19
[7]严淑珍.中药外敷法治疗小儿疮积.河北中医,1991,(4):10
[8]国家药典委员会 中华人民共和国药典一部.2005版.北京:北京化学工业出版社,2005:196
[9]江苏新医学院.中药大辞典.下册 上海:上海人民出版社。1977:1787
[10]明李时珍《本草纲目》校点本第三册.北京:人民卫生出版社,1978:1741
[11]徐国钧,徐珞珊.常用中药材品种整理和质量研究.福建科学出版社,1997.832
[12]王旭.苦杏仁混充桃仁的原因及解决办法的探讨.基层中药杂志,2001,15(3):38
[13]有地滋等.第四回天然药物0)开发七应用,夕术犷少A要旨集 1982:7
[14]庄司顺三.相七仁的化学,药理和生物化学 国外医学·中医中药分册,1987,9(4):31
[15]森重英明等.Studies on the Constituents of Persicae Semen(Ⅰ)生药学杂志,1983,37(1):46
[16]有地滋等.汉药桃仁的研究.国外医学,中医中药分册,1983,5(3):50
[17]苗和恺等.桃仁精油的化学成分研究 中成药,1992,14(2):33
[18]Choi Jae Sue et al.Antihyperhpide nic Effect of Flauonoids from Prunesdavidiana.J.Nat.Prod,1991,54(1):218
[19]国家中医药管理局《中华本草》编委会.中华本草.四册.上海:上海科技出版社,1999.75
[20]文川,徐浩,黄启福,等.活血中药对ApoE基因缺陷小鼠血脂及动脉粥样硬化斑块炎症反应的影响[J].中国 中西医结合杂志,2005,25(4):345.
[21]Park wH,Hong MY,Chung KH,et al.Effects of traditional herbal medicine,Hwaotang,on atherosclerosis using the spontaneous familial hypercholes-terolemia model,Kurosawa and K usanagi-hypercholes-terolemic rabbits and the venous thrombosis rats[J].Phytother Res,2005,19(10):846.
[22]Park WH,Shin SS,Lee YC,et al.The inhibitory effects of Silso-San-Gami-onatherosclerosis in KHC rabbits[J],Thrornb Res,2004,113(3-4):235.
[23]朱萱萱,朱芳,施荣山.桃仁、防己提取物对大鼠血小板聚集作用的研究.中医药研究,2000,16(3):44
[24]张秋海等.桃仁的研究进展.实用中西医结合杂志,1993,6(3):163
[25]朱萱萱等.桃仁、防已提取物对大鼠血小板聚集作用的研究.中医药研究,2000,16(3):45
[26]张清波等 用激光多普耳血流量仪及胆汁流量计测定桃仁提取物对肝脏微循环的影响.上海中医药杂志,1985,(7):45
[27]赵国荣等.四逆散、逍遥散及其配伍丹参或桃仁对大鼠免疫性肝损伤影响的对比研究.湖南中医学院学报,1999.19(4):9
[28]徐列明等.桃仁提取物抗实验性肝纤维化的作用观察.中国中药杂志,1994,19(8):491
[29]孙维强等.桃仁抗肝脏脂质过氧化损坏作用的实验研究,湖南中医杂志,1993,9(6):47
[30]吕文海等.桃仁炮制品的初步药理研究.中药材,1994,17(3):29
[31]张秋海等.桃仁的研究进展.实用中西医结合杂志,1993,6(3):163
[32]刘英等.炒桃仁总蛋白对小鼠B细胞功能影响的实验研究.中医药学报,2001,29(2):55
[33]国家中医药管理局《中华本草》编委会.中华本草.四册.上海:上海科技出版社,1999.75
[34]雷载权,张廷模主编.中华临床中药学(下卷).北京:人民卫生出版社,1998.1116
[35]方文贤,宋崇顺,周立孝,医用中药药理学。北京,人民卫生出版社,1998,280
[36]Chang HK,Shin MS,Yang HY,et al.Amygdalin induces apoptosis through regulation of Bax and Bcl-2 B expressions in human DU145 and LNCaP prostate cancer cells[J].Biol Pharrfl Bull,2006,29(8):1 597.
[37]Park I-tl,Yoon SH,Han LS,et al.Amygdalin inhibits genes related to cell cycle in SNU -C4 human colon cancer cells[J].Worle J Gastroenterol,2005,11(33):5 156.
[38]Kwon HY,Hong SP,Hahn DH,et al.Apoptosis induction of Persicae Semen extract in human promyelocytic leukemia(HL-60)cells[J].Arch Pharm Res,2003,26(2):157.
[39].孙秀坤,许爱娥.7种中药乙醇提取物及补骨脂素对人黑素痛YUGEN8细胞酪氨酸酶的影响[J].中华皮肤科杂志,2006,39(6):328.
[40]潘晶,宋淑云,蔡可津等.谈生桃仁炮制后对临床疗效的影响.吉林中医药,1994,(5):34
[41]吴斌等.“831”注射液治疗缺血性中风临床及实验观察.中西医结合杂志,1991,11(12):738
[42]刘成等.桃仁提取物合虫草菌丝治疗肝炎后肝硬化的观察.中医杂志,1991,32(7):20
[43]柴瑞雾等.桃仁“止咳“析议.上海中医药杂志,1991,(1):48
[44]刘乾和等.56例慢性咽炎治疗小结.上海中医药杂志,1989,(1):40
[45]田浴尘.活血化瘀法临床应用及其实验研究的进展.山东中医学院学报,1978:17
[46]周仲瑛等 泻下通奈法治疗流行性出血热少尿期86例疗效观察.江苏中医杂志,1985,6(10):27
[47]吕文海,卜永春.桃仁炮制品的初步药理研究.中药材,1994,17(3):29
[48]方文贤,宋崇顺,周立孝,医用中药药理学。北京,人民卫生出版社,1998,280
[49]崔玉荣,卢涛.以油角为原料制取精油酸[J].郑州轻工业学院学报,1992,7(2):29-34
[50]傅红,裘爱咏.分子蒸馏法制备鱼油多不饱和脂肪酸[J].无锡轻工大学学报,2002,21(6):617-621
[51]杨村,于宏奇,冯武文.分子蒸馏技术[M].北京:科学出版社,2003:79-87
[52]王明霞,黄凤洪.多不饱和脂肪酸萃取中超临界技术的应用进展[J].中国油脂,2005,30(8):60-64
[53]Michihata T,Matsud Y,Yasuo S,et al.Extraction of lipid from the reside of ika-ishiru using supercritical c bom dioxide[J].Journal of Japanese society offood science,1997,44(11):795-800
[54]陈永,赵辉,江鸿,等.混合脂肪酸的分离 化学世界,2000,6(3):156-159
[55]侯镜德,骆小明,冯建跃,等.顺-15-二十四碳烯酸的分离制备方法:中国,96 122232.8[P].
[56]Douglas G.Hayes.Effect of temperature programming on the perfor-mance of urea inclusionc ompound-based free fatty acid fractionation[J].Journal of the American Oil Chemists Society,2006,83(3):253-259
[57]Robert W,John R S.Chain separation of monounsaturated fatty acid methyl estem by argentation thin layer chromatography[J].Journal of chromatography A,2001,905(1):251-257
[58]张氽,阚建全,陈宗道.硝酸银-硅胶纯化a-亚麻酸的研究[J]离子交换与吸附,2005,21(1):47-54
[59]Rezanka T.Two-dimensional separation of fatty acids by thin-layer chromatography on urea and silver nitrate silica gel plates[J].Journal of chromatography A.1996,727(Ⅰ):147-151
[60]周兴,林成仁,饶琳,等.n-3多烯脂肪酸对血脂影响的实验研究[J].中国中医基础医学杂志,1997,3(4):29
[61]徐静,李姜溶,王健敏.多烯脂肪酸胶丸治疗高脂血症疗效观察[J].Heilongjiang medicine and pharmacy,1998,21(4):65
[62]周兴,林成仁,饶琳,等.n-3多烯脂肪酸对血小板聚、血栓形成、6-keto-PGR_(??)/TXB2比值的影响[J].中药药理与临床,1997,13(1):27
[63]Zimmer L,Delpal S,Guiloteau D,et al.Chronic n-3 polyunsaturated fatty acid deficiency alters dopamine vesicle density in the rat fomtal cortes[J].Neurosci Lett,2000,284:25
[64]Moussa M,Le Boucher J,Garcia J,et al.In vivo effects of olive oil-based lipid emulsion on lymphocyte activation in rats[J].Clin Nutr,2000,19:49
[65]Chavali SR,Forse RA.Decreased production of interleukin-6 an d pros- taglandin E2associated with inhibition of delta-5 desaturation of ome- ga-6 fatty acids in mice fed safflower oil diets supplemented with 8esa-mol[J].Prostaglandins Leukotrienes Essential Fatty Acids,1999.61:347
[66]Arbogast.BW,Neuman n JK,Arbogast LY,et al.Transient loss of serllm protective activity following short-term stress:A possible bioc hemical link between stress and atherosclerosis[J].J Psychosom Res,1994,38:871
[67]Sudheera S,Leitch J,Garg M.N-3 Polyunsaturated fatty acid supplementation alters inositol phosphate metabolism and protein kinase C activity in adult porcin cardiacmyocytes.Journal of Nutritional Biochemistry,2001,(12):7-11
[68]Leifert W.R,Dorian C.L,Jahangiri A,al e.Dietary fish oil prevents asynchronous contractility and alters Ca'-' handling in adult rat cardiomyocytes.J Nutr Biochem,2001,12(6):365-376
[69]Rinaldi B,Di Pien'o P,ViteUi MR,D'Amico M,Berrino L' Rossi F,Filippelli A.Effects of docosahexaenoic acid on calcium pathway in adult rat cardiomyocytes.Life Sci,2002,71(9):993-1004
[70]蔡双莲,李敏.多不饱和脂肪酸的研究进展.生命科学研究,2003,7(4):289-304
[71]Hu FB,Bronner L,Willett W C,et al.Fish and omega-3 fatty acid intake and risk of coronary heart disease in women.JAMA,2002,287(14):1815-1821.
[72]Iso H,Rexrode KM,Stampfer MJ,et al.Intake of fish and omega-3 fatty acids and risk of stroke in women.JAMA,2001,285(3):304-312.
[73]A review of some herbal and related products conmmonly used in Cancer patients.Jouma]of the Amercian Dretetic Associatin,1997、(10)
[74]赵索莲.等苦杏仁苷埘小鼠脾脏￡细胞活性的影响.山西医学院学报,1993,(1):14
[75]赵素莲,等苦杏仁苷对小鼠免疫功能的影响 山西医药杂志,1993,(3):166
[76]李春华,等苦杏仁苷对小鼠肝枯否细胞DNA活性及吞噬功能的影响.实用中西医结合杂志,1994,(1):7
[77]Syrigos KN et al.In vitro cytoloucrty following spedific activation of amygdalin by beta-glucosidase conjugated to aBladder cancer-associated monoclonalantibody.In J Cancer,1998,78(6):712
[78]徐列明.等.扁桃苷对大鼠肝贮脂细胞增殖和产生胶原的影响.新消化病学杂志,1997,(2):84
[79]屈燧林,等汉防己甲素、川芎嗪和苦杏仁苷对人身成纤维细胞的影响.中华肾脏病杂志,2000,16(3):186
[80]Role of the gastrointestitud microflora in amygdalin-inducedcyarude toxicity.Biochemical Pharmacology,1980,29:301
[81]王本样.现代中药药理学.天津:天津科学技术出版社,1997:1005-1007
[82]李熙民,等.苦杏仁苷药物动力学及其毒理初步研究.新药与临床,1986,5(3):141
[83]Moertel.Charles G.et al.A Pharmacologic and ToxicolgicalS tudy of Amygalin.JAMA,245591
[84]American Cancer Society latetrle-The Offical View Un-proven Remedies.Sept 10,1996
[85]Spencer PS.et al Neuolog diseases associated with ofplant components with toxic potentia]Envier Res,1993,62:106
[86]张国明,等苦杏仁苷在土体内的药物动力学.中国药理学报,1986,7(5):460
[87]Copalan venkataknshnan. et al. Exolytic hydrolysis of toxicplant glucosides by gumea pigliver cytosolic p_ glucosidase J Biol Chem. 1992. 267: 14027
[88]strugala GJ, et al. Small—Intest inal trasfer mechanism ofprunasin, the primary metabolite of the cyanogenic glycoside amygdalin. Hum Exp Toxicol, 1995, 14(11): 895
[89]Hays William S. et al Expression of cytosolic B-glucosidasein guinea pig liver cells. Hepatology, 1998, 28(1): 156
[90]GlewRH, etal. The mammalian brosd-specificity B—glucosidase in exen A, ed. B—glucosidase:Biochemistry and Molecular Biology Washington DC Araereian Chemical society , 1993: 83