光信号在微生物区分与药物抗菌活性研究中的应用
|
摘要
众所周知,通过物质对光的吸收、散射和发荧光、发磷光等现象,可以对物质进行定性和定量测定。本文主要利用从荧光分光光度计上获得的光散射信号对微生物进行了区分,利用流式细胞仪上获得的荧光信号研究了改性头孢噻肟和细胞型朊蛋白(PrP~c)的抑菌活性。具体研究内容如下:
1、利用光散射信号对微生物进行区分
光的散射信号发生在光与粒子的相互作用过程中,它与散射粒子的大小、形态、折光指数等方面有关。本文在配有偏振片的普通荧光分光光度计上保持激发和发射波长相等,通过同时扫描激发和发射单色器获得的光散射光谱对大肠杆菌Escherichia coli(E.coli)的2种不同菌株:E.coli(DH5α),E.coli(BL-21)进行了探索性研究。结果表明,偏振同步光散射可以区分、表征这两种微生物,该方法简便、灵敏、快速。
2、利用流式细胞仪上获得的荧光信号研究药物的抑菌活性
流式细胞仪既可以检测样品的散射信号,也可以检测样品的荧光信号。本文利用流式细胞仪上获得的荧光信号对药物的抑菌活性进行了研究。
(1)在头孢噻肟(Cefotaxime Sodium)7位侧链的氨基上通过戊二醛的偶联作用修饰上壳聚糖,将修饰及未修饰的头孢噻肟分别与金黄色葡萄球菌(Staphylococcus aureus,S.aureus)孵育后,用碘化丙锭(Propidium Iodide,PI)染色,然后利用流式细胞仪检测样品荧光强度的变化。结果表明,头孢噻肟改性后对金黄色葡萄球菌的抑菌活性增强了。本文同时研究了不同温度对头孢噻肟改性后抑菌活性的影响,发现在60℃下所得的改性头孢噻肟的抑菌活性最强。另外也研究了改性头孢噻肟在不同浓度和不同作用时间下与S.aureus的作用情况,这将为临床给药提供一定依据。
(2)利用流式细胞仪探索了细胞型朊蛋白(PrP~c)对金黄色葡萄球菌(Staphylococcusaureus,S.aureus)、枯草芽孢杆菌(Bacillus subtilis,B.subtilis)、巨大芽孢杆菌(Bacillusmegaterium,B.megaterium)、苏云金芽孢杆菌(Bacillus thuringiensis,B.thuringiensis)、大肠杆菌[Escherichia coli(BL-21),E.coli(BL-21)]这五种随机所选细菌的抑菌活性,结果表明,细胞型朊蛋白(PrP~c)对S.aureus和B.subtilis具有明显的抑制活性,而对B.megaterium,B.thuringiensis和E.coli(BL-21)这三种菌没有抑制活性,这将对抗菌肽的开发和细胞型朊蛋白(PrP~c)的生理功能的阐明提供一定的实验依据。
It is known that light signals through light absorption,light scattering,fluorescence and phosphorescence,could be qualitatively and quantitatively applied.In this contribution,light scattering signals obtained from fluorescence spectrophotometer were applied to differentiate microorganisms,and use the fluorescent signals from flow cytometer to study the antimicrobial activity of modified cefotaxime sodium and cellular prion protein(PrP~c).The specific content studied show as follows:
1、Light scattering signals for differentiating microorganisms
Light scattering signals occurred in the interaction process of light photon and particles which are related to the size,the shape and the refraction index of the particles.With a common fluorescence spectrophotometer that equipped polarizer by keeping excitation and emission wavelengths equal,to scan the excitation and emission monochromator,we obtained the light scattering spectra to study the two different strain of Escherichia coli(E.coli):E.coli(DH5α)and E.coli(BL-21).The results showed that polarized synchronous light scattering can differentiate this two microorganisms,with high simplicity,sensitivity and speediness.
2、The fluorescent signals from flow cytometer to study the antimicrobial activity of antimicrobial medicine
Flow cytometer can not only detect the light scattering signals,but also can detect the fluorescent signals of a sample,Thus,we use flow cytometer to study the antimicrobial activity of the antimicrobial medicine.
(1)Modified the amino at 7-side chain of cefotaxime sodium with chitosan through glutaric dialdehyde,By incubating S.aureus with cefotaxime sodium or with the cefotaxime sodium which modified with chitosan through glutaric dialdehyde respectively,Dyeing with propidium iodide and detecting the change of fluorescent intensity of the sample,We found that the antimicrobial activity of cefotaxime sodium to S.aureus is enhanced after modification.Further investigation on the influence of temperature on the antimicrobial activity of the modified cefotaxime sodium showed that cefotaxime sodium modified at 60℃has the strongest antimicrobial activity.Investigation on the action of the modified cefotaxime sodium by incubating with S.aureus at different concentration and different incubation time cound provide some basis for clinical drug administration.
(2)Investigation on the antimicrobial function of PrP~c to five randomly choosed bacteria, including Staphylococcus aureus(S.aureus),Bacillus subtilis(B.subtilis),Bacillus megaterium(B. megaterium),Bacillus thuringiensis(B.thuringiensis)and Escherichia coli(BL-21)[E.coli(BL-21)] showed that PrP~c has visible antimicrobial effect on S.aureus and B.subtilis,but no effect on the other three bacteria,B.megaterium,B.thuringiensis and E.coli(BL-21).The results will provide some experimental data for the exploring of aitimicrobial peptide and for the clarifying the physiological function of PrP~c.
1、利用光散射信号对微生物进行区分
光的散射信号发生在光与粒子的相互作用过程中,它与散射粒子的大小、形态、折光指数等方面有关。本文在配有偏振片的普通荧光分光光度计上保持激发和发射波长相等,通过同时扫描激发和发射单色器获得的光散射光谱对大肠杆菌Escherichia coli(E.coli)的2种不同菌株:E.coli(DH5α),E.coli(BL-21)进行了探索性研究。结果表明,偏振同步光散射可以区分、表征这两种微生物,该方法简便、灵敏、快速。
2、利用流式细胞仪上获得的荧光信号研究药物的抑菌活性
流式细胞仪既可以检测样品的散射信号,也可以检测样品的荧光信号。本文利用流式细胞仪上获得的荧光信号对药物的抑菌活性进行了研究。
(1)在头孢噻肟(Cefotaxime Sodium)7位侧链的氨基上通过戊二醛的偶联作用修饰上壳聚糖,将修饰及未修饰的头孢噻肟分别与金黄色葡萄球菌(Staphylococcus aureus,S.aureus)孵育后,用碘化丙锭(Propidium Iodide,PI)染色,然后利用流式细胞仪检测样品荧光强度的变化。结果表明,头孢噻肟改性后对金黄色葡萄球菌的抑菌活性增强了。本文同时研究了不同温度对头孢噻肟改性后抑菌活性的影响,发现在60℃下所得的改性头孢噻肟的抑菌活性最强。另外也研究了改性头孢噻肟在不同浓度和不同作用时间下与S.aureus的作用情况,这将为临床给药提供一定依据。
(2)利用流式细胞仪探索了细胞型朊蛋白(PrP~c)对金黄色葡萄球菌(Staphylococcusaureus,S.aureus)、枯草芽孢杆菌(Bacillus subtilis,B.subtilis)、巨大芽孢杆菌(Bacillusmegaterium,B.megaterium)、苏云金芽孢杆菌(Bacillus thuringiensis,B.thuringiensis)、大肠杆菌[Escherichia coli(BL-21),E.coli(BL-21)]这五种随机所选细菌的抑菌活性,结果表明,细胞型朊蛋白(PrP~c)对S.aureus和B.subtilis具有明显的抑制活性,而对B.megaterium,B.thuringiensis和E.coli(BL-21)这三种菌没有抑制活性,这将对抗菌肽的开发和细胞型朊蛋白(PrP~c)的生理功能的阐明提供一定的实验依据。
It is known that light signals through light absorption,light scattering,fluorescence and phosphorescence,could be qualitatively and quantitatively applied.In this contribution,light scattering signals obtained from fluorescence spectrophotometer were applied to differentiate microorganisms,and use the fluorescent signals from flow cytometer to study the antimicrobial activity of modified cefotaxime sodium and cellular prion protein(PrP~c).The specific content studied show as follows:
1、Light scattering signals for differentiating microorganisms
Light scattering signals occurred in the interaction process of light photon and particles which are related to the size,the shape and the refraction index of the particles.With a common fluorescence spectrophotometer that equipped polarizer by keeping excitation and emission wavelengths equal,to scan the excitation and emission monochromator,we obtained the light scattering spectra to study the two different strain of Escherichia coli(E.coli):E.coli(DH5α)and E.coli(BL-21).The results showed that polarized synchronous light scattering can differentiate this two microorganisms,with high simplicity,sensitivity and speediness.
2、The fluorescent signals from flow cytometer to study the antimicrobial activity of antimicrobial medicine
Flow cytometer can not only detect the light scattering signals,but also can detect the fluorescent signals of a sample,Thus,we use flow cytometer to study the antimicrobial activity of the antimicrobial medicine.
(1)Modified the amino at 7-side chain of cefotaxime sodium with chitosan through glutaric dialdehyde,By incubating S.aureus with cefotaxime sodium or with the cefotaxime sodium which modified with chitosan through glutaric dialdehyde respectively,Dyeing with propidium iodide and detecting the change of fluorescent intensity of the sample,We found that the antimicrobial activity of cefotaxime sodium to S.aureus is enhanced after modification.Further investigation on the influence of temperature on the antimicrobial activity of the modified cefotaxime sodium showed that cefotaxime sodium modified at 60℃has the strongest antimicrobial activity.Investigation on the action of the modified cefotaxime sodium by incubating with S.aureus at different concentration and different incubation time cound provide some basis for clinical drug administration.
(2)Investigation on the antimicrobial function of PrP~c to five randomly choosed bacteria, including Staphylococcus aureus(S.aureus),Bacillus subtilis(B.subtilis),Bacillus megaterium(B. megaterium),Bacillus thuringiensis(B.thuringiensis)and Escherichia coli(BL-21)[E.coli(BL-21)] showed that PrP~c has visible antimicrobial effect on S.aureus and B.subtilis,but no effect on the other three bacteria,B.megaterium,B.thuringiensis and E.coli(BL-21).The results will provide some experimental data for the exploring of aitimicrobial peptide and for the clarifying the physiological function of PrP~c.
引文
[1]李燕主编.现代药物分析与评价新技术新方法新标准指导手册.第一版.中国科技文化出版社.2005,3-4;
[2]张亚梅.药物中毒性耳聋.中华儿科杂志.2000.12,38(12):781-782.北京.中华医学会;
[3]Cuzzocrea,S.;Mazzon,E.;Dugo,L.;et al.A role for superoxide in gentamicin-mediated nephropathy in rats.Eur J Pharmacol.2002.450(1):67-76;
[4]Wallace,K.B..Doxorubicin induced cardiac mitochondrionopathy.Pharmacol Toxicol.2003.93:105-115;
[5]Galliteni,M.;Braidotli,P.;Cozzolino,M.;et al.Acute Tubulo-interstitial nephritis requiring dialysis with intermittent rifampcin use:case report.Int J Antif Organs.1999.22(7):477-479;
[6]Ravi,D.;Das,K.C..Redox-cycling of anthracyclines by thioredoxin system:increased superoxide generation and DNA damage.Cancer Chemother Pharmacol.2004.54(5):449-458;
[7]Ali,B.H..Agents ameliorating or augmenting experimental gentamicin nephrot- oxicity:some recent research.Food Chem Toxicol.2003.41(11):1447-1452;
[8]Sakac,V.;Sakac,M..Free oxygen radicals and kidney diseases - part Ⅰ.Med Pregl.2000.53(9-10):463-474;
[9]Liu,F.T.;Kelsey,S.M.;Newland,A.C.;et al..Liposomal encapsulation diminishes daunorubicin-induced generation of reactive oxygen species,depletion of ATP and necrotic cell death in human leukaemic cells.Br J Haematol.2002.117(2):333-342;
[10]Covic,A.;Goldsmith,D.J.;Segall,L.;et al..Rifampicin-induced acute renal failure:a series of 60 patients.Nephrol Dial Transplant.1998.13(4):924-929;
[11]Popa,R.;Anniko,M.;Takumida,M.;et al.Localization of nitric oxide synthase isoforms in the human cochlea.Acta Otolaryngol.2001.121(4):454-459;
[12]吾建浓,施敏凤,王灵聪,叶雪惠.万古霉素治疗葡萄球菌肺炎的疗效与肾毒性评价.中国新药与临床杂志.2005.24(3):245-247;
[13]Childs,A.C.;Phaneuf,S.L.;Dirks,A.J.,et al.Doxorubicin treatment in Vivo causes cytochrome c release and cardiomyocyte apoptosis,as well as increased mitochondrial efficiency,superoxide dismutase activity,and bcl-2:bax ratio.Cancer Res.2002.62:4592-4598;
[14]Zhou,Y.;Hileman,E.O.;Plunkett,W.;et al..Free radical stress in chronic lymphocytic leukemia cells and its role in cellular sensitivity to ROS-generating anticancer agents.Blood. 2003.101(10):4098-4104;
[15]Nelson,E.B..Kidney failure and analgesic drugs.New Engl J Med.1995.332(22):1514-1516;
[16]Hattori,S.;Yosioka,K.;Honda,M.;e t al..The 1998 report of the Japanese national registry data on pediatric end-stage renal disease patients.Pediatr Nephrol.2002.17(6):456-461;
[17]Yi,J.;Gao,F.;Shi,G.;et al..The inherent cellular level of reactive oxygen species:one of the mechanisms determining apoptotic susceptibility of leukemic cells to arsenic trioxide.Apoptosis.2002.7(3):209-215;
[18]臧宏科,徐冬梅.头孢菌素类抗生素的肾毒害及护理.山东医药.2003.43(2):66;
[19]Sone,M.;Schachern,P.A.;Paparella M.M.;Paparella,M.M..Loss of spiral ganglion cells as primary manifestation of aminoglycoside ototoxicity.Hear Res.1998.115:217-223;
[20]Conlon,B.J.,Smith,D.W..Attenuition of neomycin ototoxicity by iron chelation.Laryngoscope.1998.108(2):284;
[21]Struelens,M.J.,Byl,B.,Vinecnt,J.L..Antibiotic policyl a tool for controlling resistance of hospital pathogens.Clin.Microbiol.Infect..1995.5:519-524;
[22]Khan,S.A.;Nawaz,M.S.;Khan,A.A.;Cerniglia C.E..Transfer of Erythromycin Resistance from Poultry to Human Clinical Strains of Staphylococcus aureus.J.Clin.Microbiol..2000.38(5):1832-1838;
[23]Shannon,K.P.;French,G.L..Antibiotic resistance:effect of different criteria for classifying isolates as duplicates on apparent resistance frequencies.J.Antimicrob.Chemother..2002.49:201-204;
[24]Eavcs,D.J.;Randall,L.;Gray,D.T.;et al.Prevalence of mutations with the quinolone resistance,determining region of gyrA,gyrB,par C,and par E and association with antibiotic resistance in quinolone-resistant Salmonella enterica.Antimicrob.Agents.Chemother..2001.48:4012-4015;
[25]Wise,R..Antimicrobial resistance:paradox,actions and economics.J.Antimicrob.Chemother..2006.57:1024-1025;
[26]Gholizadeh,Y.;Couvalin,P..Acquired and intrinsic glycopeptide resistance in enterococci.Intemational J of Antimicrobial Agents.2000.16:511-517;
[27]Lin,S.Y.G.;Probert,W.;Lo,M.;Desmond,E..Rapid Detection of Isoniazid and Rifampin Resistance Mutations in Mycobacterium tuberculosis Complex from Cultures or Smear-Positive Sputa by Use of Molecular Beacons.J.Clin.Microbiol.2004.42(9):4204-4208;
[28]Katayama,J.;Okada,H.;O' Hara,K.;et al.Isolation and characterization of two plasmids that meliate macrolide resisfance in Escherichia coli:transferability and molecular properties.Biol PharmBull.1998.21:326;
[29]Lee,S.O.;Cho,Y.K.;Kim,S.Y.;Lee,E.S.;Park,S.Y.;Seo,Y.H..Comparison of Trends of Resistance Rates over 3 Years Calculated from Results for All Isolates and for the First Isolate of a Given Species from a Patient.J.Clin.Microbiol.2004.42(10):4776-4779;
[30]Leclercq,R.;Courvalia,P..Bacterial resistance to macrolide,lincosamide and streptogramin antibiotics bytarget modification.Antimicrob.Agents.Chemother..1991.35:1267-1272;
[31]Magee,J.T..Effects of duplicate and-screening isolates on surveillance of community and hospital antibiotic resistance.J.Antimicrob.Chemother..2004.54:155-162;
[32]Leclercq,R.;Dutka-Malen,S.;Brissor-Noel,A.;et al..Resistance of entercocci to aminoglycosides and glycopeptides.Clin infect Dis.1992.15:415-501;
[33]Du,Z.M.;Yang,R.E;Guo,Z.B.;Song,Y.J.;Wang,J..Identification of Staphylococcus aureus and Determination of Its Methicillin Resistance by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.Anal.Chem..2002.74:5487-5491;
[34]Llano-Sotelo,B.;Azucena,EFJr.;Kotra,L.P.;et al..Aminoglycosides modified by resistance enzymes display diminished binding to the bacterial riibosomal aminoacyl-tRNA site.Chem Biol.2002.9:455-501;
[35]赵亮,陈凌,陈贻锴.白色假丝酵母菌耐药性与耐药基因表达及蛋白酶的关系.临床检验杂志.2007.25(5):348-350;
[36]彭敬红,孙自鳙,周有利,吕军.产ESBLs大肠埃希菌的耐药性研究.现代预防医学.2007.34(19):3668-3672;
[37]贾杰.抗生素的耐药性与抗生素的应用.中国热带医学.2007.7(9):1678-1680;
[38]Emmanuelle,C.;Elaine,T..Mechanisms of antibiotic resisitence and tolerance in Streptococcus pneumoniae.Microbes and Infection.2000.2:1855-1864;
[39]杨斌爱,武改娣,马淑玲,呼萍萍.葡萄球菌的分布及耐药性分析.山西医药杂志.2007.36(9):858-859;
[40]万同己.幽门螺杆菌根除治疗的失败原因及其防治对策.中国热带医学.2007.7(9):1590-1592;
[41]Liao,Q.G.,Li,Y.F.,Huang,C.Z..A light scattering and fluorescence emission coupled ratiometry using the interaction of functional CdS quantum dots with aminoglycoside antibiotics as a model system.Talanta.2007.71:567-572;
[42]Huang,C.Z.,Li,Y.F.,Feng,P..A spectrophotometric study on the interaction of neutral red with double-stranded DNA in large excess.Anal.Chim Acta.2001.55:321-328;
[43]Long,Y.J.,Li,Y.F.,Huang,C.Z..A wide dynamic range detection of biopolymer medicines with resonance light scattering and absorption ratiometry.Anal.Chim.Acta..2005.552: 175-181;
[44]Huang,C.Z.,Li,Y.F.,Huang,X.H.,Li,M..Interactions of Janus Green B with double stranded DNA and the determination of DNA based on the measurement of enhanced resonance light scatteringAnalyst.2000.125:1267-1272;
[45]Long,Y.F.,Huang,C.Z..Spectral studies on the interaction of Amido black 10B with DNA in the presence of cetyltrimethylammonium bromide.Talanta.2007.71:1939-1943;
[46]易平贵,刘俊峰,商志才,俞庆森.荧光光谱法研究亚甲基蓝与蛋白质的结合反应.光谱学与光谱分析.2001.21(6):826-828;
[47]肖厚荣,盛良全,施春华,徐小龙,解永树,刘清亮.水杨酸与牛血清蛋白相互作用的荧光光谱研究.光谱学与光谱分析.2004.24(1):78-81;
[48]李改仙,李建晴,魏玉霞,董川.四碘荧光素与牛血清白蛋白相互作用的荧光光谱研究.光谱学与光谱分析.2005.25(8):1277-1280;
[49]颜承农,童金强,熊丹,刘义,潘祖亭.荧光光谱法研究培氟沙星与牛血清白蛋白结合反应特征.分析化学.2006.34(6):796-800;
[50]陈晓波,康栋国,李崧,赵承易,陈晓珊,丁焕平.利福平与人血清白蛋白作用的荧光光谱.光谱学与光谱分析.2006.26(4):674-677;
[51]林伟,彭亦如,陈奎治,翁家宝,徐国兴.光谱分析法研究四磺酸酞菁金属配合物与牛血清白蛋白的结合作用.分析化学.2006.34(3):411-414;
[52]马国欣,王成龙,范多旺,邢达,钱隆,王锦辉,刘颂豪.红外光谱法测定农药中吡虫啉含量.光谱学与光谱分析.2006.26(3):434-437;
[53]李占龙,周密,左剑,王云鹏,姜永恒,里佐威.红外光谱和拉曼光谱法分析玉米种子的成分.分析化学.2007.35(11):1636-1638;
[54]陈奎治,彭亦如,林伟,曹育红,徐国兴,翁家宝.光谱分析法研究八羧酸酞菁铝配合物与牛血清白蛋白的结合作用.光谱学与光谱分析.2007.27(1):84-87;
[55]李燕,吴然然,于佰华,王俊德.红外光谱在中药定性定量分析中的应用.光谱学与光谱分析.2006.26(10):1846-1849;
[56]武汉大学化学系主编.仪器分析.第一版.北京:高等教育出版社.2001.11-36;
[57]李原芳,黄承志,胡小莉.共振光散射技术的原理及其在生化研究和分析中的应用.分析化学.1998.26(12):1508-1515;
[58]梁柱.光学原理教程.第一版.北京:北京航空航天大学出版社.2005.65-163;
[59]R.Pecora.Dynamic Light Scattering.Plenum Press.New York.1985;
[60]左榘.激光散射原理及在高分子科学中的应用.第一版.郑州.河南科学技术出版社.1994.40-259;
[61]杨文治.物理化学实验技术.第一版.北京:北京大学出版社.1992.317-327;
[62]Huang,C.Z.;Li,Y.F..Resonance light scattering technique used for biochemical and pharmaceutical analysis.Anal.Chim.Acta.2003.500.105-117;
[63]Pastemack,R.F.;Bustamante,C.;Collings,P.J.;Giannetto,A.;Gibbs,E.J..Porphyrin Assemblies on DNA as Studied by a Resonance Light-Scattering Technique.J.Am.Chem.Soc..1993.115(13):5393-5399;
[64]黄剑平,梅平,陈芳,何治柯.Ru(bpy)_2 PIP(Ⅳ)~(2+)与DNA作用的共振光散射光谱分析及应用.武汉大学学报(物理版).2007.53(4):426-430;
[65]万新军,刘金水,王伦.吖啶橙共振光散射法测定脱氧核糖核酸.光谱学与光谱分析.2005.25(5):754-756.
[66]冯硕,李正平,张淑红,方正.阿霉素与DNA作用的共振光散射研究及在DNA分析中的应用.光谱学与光谱分析.2006.26(4):707-710;
[67]Huang,C.Z.;Li,Y.F.;Deng,S.Y.;Liu,S.R..Assembly of Methylene Blue on Nucleic Acid Template as Studied by Resonance Light-Scattering Technique and Determination of Nucleic Acids of Nanogram.Bull.Chem.Soc.Jpn..1999.72.1501-1508;
[68]冯宁川,林枫,刘利军,刘建平.次甲基绿.溴代十六烷基三甲基铵体系共振光散射法测定脱氧核糖核酸.中国卫生检验杂志.2005.15(2):172-173;
[69]Li,Y.F.;Shu,W.Q.;Feng,P.;Huang,C.Z.;Li,M..Determination of DNA with Cetyltrimethylammonium Bromide by the Measurement of Resonance Light Scattering.Anal.Sci..2001.17:693-696;
[70]李波,于京华,谭云,程晓亮,朱元娜.共振瑞利散射法测定核酸探针的发展及应用.济南大学学报(自然科学版).2007.21(3):210-214;
[71]曾金祥,陈小明,蔡昌群,罗和安.碱性品红共振光散射法测定DNA研究.分析科学学.报.2005.21(6):664-666;
[72]陈展光,丁卫锋,韩雅莉,卢峰,廖栩泓,陈小明.两性离子表面活性剂作为共振光散射探针测定脱氧核糖核酸.分析化学.2005.33(4):519-522;
[73]冯硕,李正平,吴秋华,方正,张淑红.以多胺为探针的DNA共振光散射光谱法测定.理化检验-化学分册.2007.43(7):555-557;
[74]Li,Y.F.;Huang,C.Z.;Huang,X.H.;Li,M..Determination of DNA by its enhancement effect of resonance light scattering by azur A.Anal.Chim.Acta..2001.429:311-319;
[75]Huang,C.Z.;Li,K.A.;Tong,S.Y..Determination of Nanograms of Nucleic Acids by Their Enhancement Effect on the Resonance Light Scattering of the Cobalt(Ⅱ)/4-[(5-Chloro-2-pyridyl)azo]-1,3-diaminobenzene Complex.Anal.Chem..1997.69:514-520;
[76]Huang,C.Z.;Li,Y.F.;Liu,X.D..Determination of nucleic acids at nanogram levels with safranine T by a resonance light-scattering technique.Anal:Chim.Acta..1998.375:89-97;
[77]Huang,C.Z.;Li,K.A.;Tong,S.Y..Determination of Nucleic Acids by a Resonance Light-Scattering Technique with α,β,γ,δ-Tetrakis[4-(trimethylammoniumyl)phenyl]porphine.Anal.Chem..1996.68:2259-2263;
[78]Long,Y.F.;Huang,C.Z.;Li,Y.F..Hybridization Detection of DNA by Measuring Organic Small Molecule Amplified Resonance Light Scattering Signals.J.Phys.Chem.B..2007.111(17):4535-4538;
[79]Li,Y.F.;Huang,C.Z.;Huang,X.H.;Li,M..Determination of Proteins Based on Their Resonance Light Scattering Enhancement Effect on Alcian Blue 8GX.Anal.Sci..2000.16:1249-1254;
[80]陈红旗,马娟,刁雪莲,王伦.PbS-PAA复合纳米粒子共振光散射法测定蛋白质.分析测试学报.2005.24(6):56-58;
[81]干宁,候琳熙,,李天华,徐伟民,周雄.共振瑞利散射测定痕量甲胎蛋白含量研究.2007.29(2):73-75;
[82]陈丹丹,张涛,任丽萍,石登荣,于金莲.光谱法研究钙红-Cu(Ⅱ)络合物与蛋白质的相互作用及其应用.光谱学与光谱分析.2006.26(4):686-689;
[83]杨新玲,王恩波,刘霞,吴惠毅.丽春红S共振光散射光谱法测定尿蛋白.临床检验杂志.2007.25(3):180-181;
[84]汪新,刘金水,杨小红,王伦.茜素黄GG共振光谱散射法测定人血清白蛋白.光谱实验室.2006.23(2):393-397;
[85]林旭聪,吴孟辉,郭良洽,谢增鸿.新的光散射体系测定蛋白质的研究.光谱学与光谱分析.2007.27(8):1587-1590;
[86]栾吉梅,张晓东.有机染料共振光散射法测定蛋白质的研究进展.理化检验-化学分册.2007.43(2):159-163;
[87]Li,Y.F.;Huang,C.Z.;Li,M..Anal.Sci..A Resonance Light-Scattering Determination of Proteins with Fast Green FCF.2002.18:177-181;
[88]张爱梅,刘妮娜.靛蓝胭脂红共振瑞利散射法测定蛋白质.分析科学学报.2006.22(3):327-329;
[89]Yang,C.X.;Li,Y.F.;Huang,C.Z..Anal.Sci..Determination of Protein with Fast Red VR by a Corrected Resonance Light-Scattering Technique.2003.19:211-215;
[90]王守兴,王峰,黄薇.共振光散射测定蛋白质的新方法.分析科学学报.2007.23(4):464-466;
[91]曾铭,李树伟,王小红.共振散射光谱法研究人血清白蛋白与二甲酚橙的反应.理化检验-化学分册.2007.43(2):93-101;
[92]邵辉莹,张爱梅.甲基蓝体系共振瑞利散射法测定蛋白质.食品科学.2006.27(3):199-201;
[93]Huang,C.Z.;Li,Y.F.;Mao,J.G.;Tan,D.G..Determination of protein concentration by enhancement of the preresonance light-scattering of α,β,γ,δ-tetrakis(5-sulfothienyl)porphine.Analyst.1998.123:1401-1406;
[94]Huang,C.Z.;Li,Y.F.;Feng,P..Determination of proteins with α,β,γ,δ-tetrakis(4-sulfophenyl)porphine by measuring the enhanced resonance light scattering at the air/liquid interface.Anal.Chim.Acta..2001.443:73-80;
[95]Huang,C.Z.;Liu,Y.;Wang,Y.H.;Guo,H.P..Resonance light scattering imaging detection of proteins with α,β,γ,δ-tetrakis(p-sulfophenyl)porphyrin.Anal.Biochem..2003.321:236-243;
[96]Huang,C.Z.;Pang,X.B.;Li,Y.F.;Long,Y.J..A resonance light scattering ratiometry applied for binding study of organic small molecules with biopolymer.Talanta.2006.69:180-186;
[97]贾桂芳,王秋霞,孙英,周志强.吡虫啉与脱氧核糖核酸相互作用机理及其影响因素环境化学.2005.24(4):430-433;
[98]Feng,P.;Shu,W.Q.;Huang,C.Z.;Li,Y.F..Total Internal Reflected Resonance Light Scattering Determination of Chlortetracycline in Body Fluid with the Complex Cation of Chlortetracycline-Europium-Trioctyl Phosphine Oxide at the Water/Tetrachloromethane Interface.Anal.Chem..2001.73.4307-4312;
[99]吴呈珂,冯素玲,樊静.光谱法研究盐酸多塞平与固绿的相互作用及其分析应用.光谱学与光谱分析.2007.27(12):2490-2493;
[100]曾铭,李树伟,邓嘉利,李小燕,赵可清.槲皮素的共振光散射光谱研究光谱学与光谱分析.2006.26(2):317-320;
[101]衷明华.抗坏血酸的共振光散射法测定.食品科学.2008.29(1):239-240;
[102]Wu,L.P.;Li,Y.F.;Huang,C.Z.;Zhang,Q..Visual Detection of Sudan Dyes Based on the Plasmon Resonance Light Scattering Signals of Silver Nanoparticles.Anal.Chem..2006.78:5570-5577;
[103]黄明元,彭晓君,李延志.共振光散射法检测辣椒及其制品中苏丹红Ⅰ.现代预防医学.2007.34(3):528-529;
[104]杨胜园,吕昌银,陆松,于军晖,杨慧仙.苏丹红Ⅰ-甲醇体系的共振光散射光谱研究及其应用.分析科学学报.2007.23(6):739-740;
[105]韩丽娟,李贵荣,谭广辉,杨慧仙,贺冬秀,于军辉,王志湘.共振光散射法测定痕量甲醛.中国卫生检验杂志.2006.16(2):189-190;
[106]李娜,刘玮炜,周洪英,周丽华,赵跃强.共振散射法测定中草药中的微量铬.分析实验室.2007.26(6):85-87;
[107]陈兰化,刘发光.共振散射光谱法测定水中铬(Ⅵ).中国卫生检验杂志.2006.16(2):205-206;
[108]杨静,廖力夫,杨江柳,袁金涛,刘传湘.光化学催化动力学共振光散射法测定痕量铜.分析实验室.2007.26(3):110-113;
[109]贺冬秀,李贵荣,陈运生,刘运美.健那绿共振光散射法测定水样中痕量铬(Ⅵ).分析科学学报.2006.22(3):336-338;
[110]谢济运,蒋治良,钟福新,冯忠伟,郭秀姣.血细胞的光谱特性研究.分析测试技术与仪器.2000.6(3):178-181;
[111]彭一兵,谢济运,蒋治良.人红细胞的共振散射光谱分析.广西科学.2001.8(3):197-198;
[112]谢济运,蒋治良.柑桔溃疡菌的共振散射光谱.物理化学学报.2001.17(5):406-411;
[113]杨明媚,蒋治良.兔红细胞的共振散射光谱研究.光谱学与光谱分析.2002.22(2):204-208;
[114]廖祖荷,罗杨合,蒋治良,谢济运.大肠杆菌的共振散射光谱研究.分析测试技术与仪器.2003.9(2):65-69;
[115]陈绍芬,黄承志,谭克俊.光散射法快速灵敏测定和表征啤酒酵母.高等学校化学学报.2006.27(6):1-5;
[116]左连富.流式细胞术与生物医学.第一版.沈阳:辽宁科学技术出版社.1996.1;
[117]王书奎,周振英.实用流式细胞术彩色图谱.第一版.上海:第二军医大学出版社.2004.2-3;
[118]A' LVAREZ-BARRIENTOS,A.,ARROYO,J.,CANTO' N,R.,NOMBELA,C.,SA'NCHEZ-PE'REZ,M..Applications of Flow Cytometry to Clinical Microbiology.CLIN.MICROBIOL.REV.2000.13(2):167-195;
[119]宝福凯.流式细胞术在免疫学中的应用.广东科技.1996.(9):5-6;
[120]Tsumagari,S.;Otani,I.;Tanemura,K.;Namba,S.;Ohtaki,T.;Kamata,H.;Kanayama,K..Characterization of CD34+ Cells from Canine Umbilical Cord Blood,Bone Marrow Leukocytes,and Peripheral Blood by Flow Cytometric Analysis.J.Vet.Med.Sci.2007.69(11):1207-1209;
[121]李成海,鲁心安,高锋,胡雷光.用流式细胞仪监测放疗肿瘤患者淋巴细胞亚群.中华放射医学与防护杂志.2007.27(3):255-256;
[122]周克元,张月飞,蔡康荣,罗国庆,张群洲,凌光鑫.流式细胞仪检测52例鼻咽癌外周血T淋巴细胞亚群.中国实验诊断学.1997.1(1):38-39;
[123]赵宏丽,孙晓慧,赵俊芳,李孟娟,丁素光.流式细胞仪对系统性红斑狼疮患者CD~(8+)T 淋巴细胞亚群的检测.中国中西医结合皮肤性病学杂志.2005.4(1):7-9;
[124]杨春莉,裘宇容.流式细胞仪对系统性红斑狼疮患者T淋巴细胞亚群及NK细胞的检测.第一军医大学学报.2000.20(3):274-275;
[125]郭华章,殷缨,刘建,胡蕴玉,张盈华.流式细胞仪检测重组合异种骨移植患者外周血T 淋巴细胞亚群的变化.第四军医大学学报.1998.19(5):537-538;
[126]钱叶勇,李炎唐,敖建华,李岩.流式细胞仪检测肾移植后淋巴细胞亚群的临床意义.临 床泌尿外科杂志.1996.11(4):240-242;
[127]吴福国,邱翠娥,韩晓琳,朱立平,蒋明.类风湿性关节炎患者2H~(4+)T淋巴细胞亚群的流式细胞仪分析.中国免疫学杂志.1999.15(3):132-134;
[128]Kandathil,A.J.;Kannangai,R.;David,S.;Nithyanandam,G.;Solomon,S.;Balakrishnan,P.;Abraham,O.C.;Subramanian,S.;Rupali,P.;Verghese,V.P.;Pulimood,S.;Sridharan,G..Comparison of Microcapillary Cytometry Technology and Flow Cytometry for CD4~+and CD8~+T-Cell Estimation.Clin.Diagn.Lab.Immunol..2005.12(8):1006-1009;
[129]王甫厚,陈绍先.用流式细胞仪测定细胞毒.中国实验临床免疫学杂志.1991.3(1):45-50;
[130]Garcia,J.A.;Monteserin,M.C.;Delgado,I.;et al..Aberrant immunophenotypes detected by flow cytometry in acute lymphoblastic leukemia..Leuk.Lymphoma.2000.36:275-284;
[131]Ito,S.;Ishida,Y.;Murai,K.;et al..Flow cytometric analysis of aberrant antigen expression of blasts using CD45 blast gating for minimal residual disease in acute leukemia and high-risk myelodys-plastic syndrome.Leuk.Res..2001.25(3):205-211;
[132]Stelzer,G.T.;Shults,K.E.;Loken,M.R..CD45 gating for routine flow cytometric analysis of human bone marrow specimens.Ann NY Acad Sci.1993.677:265-280;
[133]Jennings,C.D.;Foon,K.A..Recent advances in flow cytometry:Application to the diagnosis of hematologic malignancy.Blood.1997.90(8):2863-2892;
[134]赵丽,周兰霞,陆莉,张晓薇.141例白血病三色流式细胞术免疫分型研究.中国免疫学杂志.2005.21(9):717-718;
[135]Borowitz,M.J.;Guenther,K.L.;Shults,K.E.;et al..Immunophenotyping of acute leukemia by flow cytometric analysis use of CD45 and right-angle light scatter to gate on leukemic blasts in three-color-analysis.Am J Clin Pathol.1993.100:534-540;
[135]Rogelio,P.A.;Lina,R.G.;Norma,L.S.;et al..Flow cytometric analysis of cell-surface and intracellular antigens in the diagnosis of acute leukemia.Am.J.Hematol..2001.68:69-74;
[136]Browitz,M.J.;Guenther,K.E.;et al..Immunophenotyping of acute leukemia by flow cytometric analysis,Use of CD45 and right-angle light scatter to gate on leukemia blasts in three-color analysis.Am.J.Clin.Pathol..1993.100:534-540;
[137]Drenou,B.;Fardel,O.;Fauchet,R.;et al..Flow cytometry:application for the diagnosis and the follow-up of hematological malignancies.Ann.Biol.Clin..2002.60(6):663-672;
[139]万岁桂,巩文玉,孙雪静,徐娟,田丁.86例儿童急性淋巴细胞白血病三流式细胞术免疫分型研究.中华血液学杂志.2002.23(2):83-86;
[140]张锑,赵玲玲,张春青,徐从高.多参数流式细胞术对外周血白血病免疫分型的研究.细胞与分子免疫学杂志.2004.20(2):225-227;
[141]陈葆国,纪东辉,褚邦勇,张丽,罗文达.多参数流式细胞术鉴别诊断单核细胞相关性白 血病的意义.中国免疫学杂志.2006.22(3):237-240;
[142]胡英萍,徐瑞龙,王香梅,郑昭璟,庄顺红.多色流式细胞术测定急性白血病免疫表型的特征及意义.检验医学.2006.21(5):533-536;
[143]沈红强,汤永民,杨世隆,钱伯芹,宋华,石淑文,徐卫群.多色流式细胞术在222例儿童急性白血病免疫分型中的应用.中华儿科杂志.2003.41(5):334-337;
[144]吴雨洁,李建勇,宋君红,郑文娟,仇海荣,张建富,朱广荣.四色流式细胞术-免疫分型在成人急性髓系白血病诊断中的应用.中华检验医学杂志.2006.29(8):750-752;
[145]Campans,D.;Coustan-Smith,E..Detection of minimal residual disease in acute leukemia by flow cytometry.Cytometry.1999.38:139-152;
[146]刘文生,王随照.微小残留白血病细胞的流式细胞术检测研究.临床检验杂志.2001.19(2):95-96;
[147]邱莲女,周永列,林明,夏晓华,皇甫梅生.流式细胞仪中CD45辅助设门在白血病免疫分型分析中的作用.临床检验杂志.2001.19(1):39-40;
[148]Paredes-Aguilera,R.;Romero-Guzman,L.;Lopez-Santiago,N.;et al..Flow cytometry analysis of cell-surface and intracellular antigens in the diagnosis of acute leukemial.Am.J.Hematol..2001.68:69-74;
[149]王文平,杨进波.流式细胞仪在白血病临床研究中的应用.中国医药导报.2006.3(26):152-153;
[150]Goustan-Smith,E.;Sancho,J.;Behm,F.G.;et al..Prognostic importance of measuring early clearance of leukemic cells by flow cytometry in childhood acute lymphoblastic leukemia.Blood.2002.100:52-58;
[151]Farahat,N.;Morilla,A..Detection of minimal residual disease in B-lineage acute lymphoblastic leukaemia by quantitative flow cytometry.Br.J.Haematol..1998.101:158-164;
[152]赵成艳,高泽斌,马荣,王忠利,何晓琳,姜风全,孙洪军.流式细胞术分析淋巴瘤穿刺液免疫表型的临床意义.中国免疫学杂志.2004.20(3):209-210;
[153]Li,S.;Juco,J.;Mann,K.P.;et al..Flow cytometry in the differential diagnosis of lymphocyte-rich thymoma from p recursor T-cell acute lymphoblastic leukemia/lymphoblastic lymphoma.Am.J.Pathol..2004.121:268-274;
[154]Young,N.A.;Al-Saleem,T.I.;Ehya,H.;et al..Utilization of fine-needle aspiration cytology and flow cytometry in the diagnosis and subclassification of primary and recurrent lymphoma.Cancer.1998.84:252-261;
[155]Gratama,J.W.;Orfao,A..Flow cytometric enumeration of CD34~+ hematopoietic stem and progenitor cells.Cytometry.1998.34:128-142;
[156]Brecher,M.E.;Sims,L.;Schmitz,J.;et al..North American multicenter study on flow cytometric enumeration of CD34~+ hematopoietic stem cells.J.hematotherapy.1996.5:227-236;
[157]Michelson,A.D..Flow cytometric analysis ofplatelets.Vox Sang.2000.78:137-142.
[158]Tomer,A..Platelet activation as a marker for in vivo prothrombotic activity:detection by flow cytometry.J.Biol.Regul.Homeost.Agents..2004.18:172-177;
[159]Michelson,A.D..Flow cytometric assessment of glycoprotein movements in platelets.Platelets.1997.8:105-115;
[160]Garcia-Martinez,M.C.;Labios,M.;Herme-Negildo,C.;et al..The effect of hormone replacement therapy on Ca~(2+)mobilization and P-selectin(CD62P)expression in platelets examined under flow cytometry.Blood.Coagul.Fibrinolysis..2004.15:1-8;
[161]Paul,H.;Allan,H.;Donna,G.;et al..Immunoplatelet counting:a proposed new reference procedure.British Joumal of Haematology.2000.108:228;
[162]Corash,L..Measurement of platelet activatiou by gluoresccnceactivated flow cytometry.Blood cells.1990.16:97;
[163]Shattil,S.J.;Cunningham,M.;Hoxie,J.A.;et al..Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry.Blood.1987.70:307-315;
[164]Jennings,L.K.;Ashmun,R.A.;Wang,W.C.;et al..Analysis of human platelet glycoproteins Ⅱ b-Ⅲa and Glanzamann's thrombasthenin in whole blood by flow cytometry.Blood.1996.68:173-179;
[165]Davies,T.A.;Drotts,D.J.;Weil,C.J.;et al..Flow cytometric measurements of cytoplasmic calcium changes in human platelet.Cytometry.1988.9:138-142;
[166]Johnston,G.L.;Pickeet,E.B.;McEver,R.P.;et al..Heterogeneity of platelet secretion in response thrombin demonstrated by fluorescence flow cytometry.Blood.1987.69:1401-1413;
[167]Ault,K.A..Flow cytometric measurement of platelet function and reticulated platelets.Ann.NY.Acad.Sci..1993.677:293-308;
[168]Gordon,N.;Thorn,J.;Cole,C.;et al..Rapid detection of hereditary and acquired platelet storage pool deficiency by flow cytometry.Br.J.Haematol..1995.89:117-123;
[169]陈军浩,张葵.流式细胞仪计数网织血小板及初步临床应用.临床检验杂志.2001.19(6):337-338;
[170]Michelson,A.D..A clinical test of platelet function.Blood.1996.87:4925-4936;
[171]丁建萍,于琳,申鲁生,王先觉,张俊芳.流式细胞术检测化疗后患儿外周血网织红细胞成熟度的临床意义.临床儿科杂志.2001.19(6):367;
[172]向晓娟,邓承祺,牛挺,雷松,孟文彤.流式细胞术检测网织血小板.中华血液学杂 志.2000.21(9):501;
[173]曹永献,姚远,张飚,于秀英,陈颖,李海霞.流式细胞仪计数网织红细胞方法评价.检验医学.2004.19(3):200-202;
[174]翟中和,王喜中,丁明孝.细胞生物学.第一版.北京:高等教育出版社.2000.358;
[175]Gong,J.;Tragano,E;Darzynkiewicz,Z..Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1.Exp.Cell.Res..1995.220(1):226-231;
[176]谢大兴,吴剑宏,冯永东,李小兰,陶德定,龚建平.细胞周期检测点的流式细胞术分析.癌症.2005.24(9):1151-1155;
[177]徐萌,李金瀚.肺癌耐药发生和细胞周期改变的流式细胞术分析.中华肿瘤杂志.2000.22(5):385-388;
[178]翟中和,王喜中,丁明孝.细胞生物学.第一版.北京:高等教育出版社.2000.454;
[179]Ibuki,Y.;Goto,R..Dysregulation of apoptosis by benzene metabolites and their relationships with carcinogenesis.Biochim.Biophys.Acta..2004.1690.11-21;
[180]Yasuda,N.;Matzno,S.;Iwano,C.;Nishikata,M.;Matsuyama,K..J.Pharm.Biomed.Anal..Evaluation of apoptosis and necrosis induced by statins using fluorescence-enhanced flow cytometry.2005.39:712-717;
[181]Fishman-Lobell,J.;Tsui,P.;Reddy,M.;DiPrinzio,R.;Eichman,C.;Sweet,R.W.;Truneh,A..CD4 mAb induced apoptosis of peripheral T cells:multiparameter subpopulation analysis by flow cytometry using Attractors.J.Immunol.Methods..2001.257:71-82;
[182]David,W.;Hedley,M.D..The Cellular Physiology of Apoptosis Mapped by Multilaser Flow Cytometry.Clin.Immunol.Newslett.1998.18(1/2):1-7;
[183]Michael,G.O..Investigating the relationship between the cell cycle and apoptosis using flow cytometry.J.Immunol.Methods..2002.265:73-80;
[184]冉立伟,谭卫明,谭升顺,王万卷,邱实,刘平.流式细胞仪测量组胺对人角质形成细胞内游离Ca~(2+)的影响.中国皮肤性病学杂志.2005.19(2):75-76;
[185]Scott,W.B.;Bruce,S.E.;Fritz,W.K.;Fredine,T.L.;Eric,R.P.;John,T.R.;Larry,A.S..Analysis of Free Intracellular Calcium by Flow Cytometry:Multiparameter and Pharmacologic Applications.METHODS.2000.21:221-230;
[186]Siegl,E.;Nebe,B.;Blunk,H.;Rychly,J..Detection of mitogen induced stimulation of leukocytes from the rainbow trout(Oncorhynchus mykiss)by flow cytometric analysis of intracellular calcium.Comp.Biochem.Physiol.A.1998.119:915-923;
[187]Adachi,T.;Tsubata,T..FRET-based Ca~(2+)measurement in B lymphocyte by flow cytometry and confocal microscopy.Biochem.Biophys.Res.Commun..2008.367:377-382;
[188]周蓓,黄淑霞,周红霞.流式细胞术在药学研究中的应用.中国医院药学杂志.2006.26(3):326-328;
[189]郑国强,刘叙仪,韩复生,徐光炜.阿霉素耐药性研究与流式细胞分析.中国肺癌杂志.2002.5(1):28-30;
[190]叶传忠,陈仕平,裴雪涛,冯凯,李梁.膀胱癌多药耐药的流式细胞术分析.中华实验外科杂志.2000.17(1):90;
[191]章小平,杨红枚,鲁功成.流式细胞仪在膀胱癌多药耐受研究中的应用.临床泌尿外科杂志.2000.15(9):417-419;
[192]褚忠华,陈涛,王捷,区庆嘉,赵海燕,魏菁,刘建平.体外化疗药敏试验系统联合流式细胞仪在胃肠肿瘤化疗中的应用.中华胃肠外科杂志.2003.6(2):120-123;
[193]尹格平,李强,陈诵芬,孙晓明,吴战英,朱彤宇.流式细胞术检测化疗前后卵巢癌细胞凋亡的临床意义.癌症.2000.19(4):383-386;
[194]Mansour,J.D.,J.A.Robson,C.W.Arndt,and T.H.Schulte.Detection of Escherichia coli in blood using flow cytometry.Cytometry.1985.6:186-190;
[195]Clarke,R.G.,Pinder,A.C..Improved detection of bacteria by flow cytometry using a combination of antibody and viability markers.J.Appl.Microbiol..1998.84:577-584;
[196]Bowden,R.A.,A.Cloeckaert,M.S.Zygrnunt,S.Bernard,and G.Dubray.Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry.Infect.Immun..1995.63:3945-3952;
[197]Cheek,R.F.,I.Olszak,S.Madoff,and F.I.Preffer..In vitro detection of Mycoplasma fermentans binding to B-lymphocytes in fresh peripheral blood using flow cytometry.Cytometry.1997.28:90-95;
[198]Patrick,S.,L.D.Stewart,N.Damani,K.G.Wilson,D.A.Lutton,M.J.Larkin,I.Poxton,and R.Brown..Immunological detection of Bacteroides fragilis in clinical samples.J.Med.Microbiol..1995.43:99-109;
[199]Bhushan,R.,C.Kirkham,S.Sethi,T.F.Murphy..Antigenic characterization and analysis of the human immune response to outer membrane protein E of Branhamella catarrhalis.Infect.Immun..1997.65:2668-2675;
[200]Ozanne,V.,A.Ortalo-Magne,A.Vercellone,J.J.Foumie,and M.Daffe..Cytometric detection of mycobacterial surface antigens:exposure of mannosyl epitopes and of the arabinan segment of arabinomannans.J.Bacteriol..1996.178:7254-7259;
[201]Ingram,M.,T.J.Cleary,B.J.Price,and A.Castro..Rapid detection of Legionella pneumophila by flow cytometry.Cytometry.1982.3:134-147;
[202]Hughes,E.E.,J.M.Matthews-Greer,and H.E.J.Gilleland.Analysis by flow cytometry of surface-exposed epitopes of outer membrane protein F of Pseudomonas aeruginosa.Can.J.Microbiol..1996.42:859-862;
[203]Chaffin,W.L.,Ringler,L.and Larsen,H.S..Interactions of monospecific antisera with cell surface determinants of Candida albicans.Infect.Infect.Immun..1998.56:3294-3296;
[204]Groschel,D.H..Methods,reagents and media:new technology in clinical microbiology.Infect.Control..1983.4:232-233;
[205]Han,Y.,Kanbe,T.,Cherniak,R.,Cutler,J.E..Biochemical characterization of Candida albicans epitopes that can elicit protective and nonprotective antibodies.Infect.Immun..1997.65:4100-4107;
[206]Williams,K.M.,Sacci,J.B.,Anthony,R.L..Characterization and quantitation of membrane antigens of New World Leishmania species by using monoclonal antibodies in Western blot and flow microfluorometric assays.J.Protozool.1986.33:490-497;
[207]Flores,B.M.,Garcia,C.A.,Stamm,W.E.,Torian,B.E..Differentiation of Naegleria fowleri from Acanthamoeba species by using monoclonal antibodies and flow cytometry.J.Clin.Microbiol..1990.28:1999-2005;
[208]Lehman,J.M.,Friedrich,T.D.,Laffm,J..Quantitation of simian virus 40 T-antigen correlated with the cell cycle of permissive and nonpermissive ceils.Cytometry.1993.14:401-410;
[209]周德庆.微生物学教程.第二版.北京:高等教育出版社.2002.3-355;
[1]贾文祥.医学微生物学.第一版.成都:四川大学出版社.2005.103-107;
[2]孙宗科,张伟,陈西平.应用飞行时间质谱仪快速鉴定细菌的初步研究.卫生研究.2004.33(5):552-554;
[3]张晖,林大榕.滤膜荧光法检测大肠菌群及其与标准滤膜法的比较.南京铁道医学院学报.1998.17(1):23-25;
[4]Bartlett M,Huang G,Larcom L,et al.Measurement of particle size distribution in mammalian cells in vitro by use of polarized light spectroscopy.Appl Opt.2004.43(6):1296-1307;
[5]Bronk B V,Druger S D,Czege J,et al.Measuring diameters of rod-shaped bacteria in vivo with polarized light scattering.Biophys.J.1995.69(3):1170-1177;
[6]Bronk B V,Van de Merwe W P,Stanley M.In vivo measure of average bacterial cell size from a polarized light scattering function.Cytometry.1992.13(2):155-162;
[7]Van de Merwe W P,Li Z Z,Bronk B V,et al.Polarized light scattering for rapid observation of bacterial size changes.Biophy.J.1997.73(1):500-506;
[8]Bronk B V,Li Z Z,Czege J.Polarized light scattering as a rapid and sensitive assay for metal toxicity to bacteria.J.Appl.Toxicol.2001.21(2):107-113;
[9]Nicolini C,Diaspro A,Bertolotto M,et al.Changes in DNA superhelical density monitored by polarized light scattering.Biophys.Res.Commun.1991.177(3):1313-1318;
[10]赵小辉,黄承志.蛋白质与十二烷基硫酸钠相互作用的光散射偏振特性.科学通报.2006.51(23):2729-2732;
[11]Feng Hao,Colleen L.Nehl,Jason H.Hafner,et al.Plasmon Resonances of a Gold Nanostar.Nano Lett.2007.7(3):729-732;
[12]Yguerabide,J.;Yguerabide,E.E..Light-Scattering Submicroscopic Particles as Highly Fluorescent Analogs and Their Use as Tracer Labels in Clinical and Biological Applications.Anal Biochem.1998.262:137-156;
[1]韩崧,王淑民,李鑫,闫美玲,柴栋,方翼.4种β-内酰胺类抗生素对临床分离致病菌的体外抗菌活性.中国抗生素杂志.2007.32(6):381-383;
[2]孙民,项明洁,韩立忠,倪语星.11种抗生素对革兰阴性杆菌抗菌活性的比较.中国人兽共患病杂志.2003.19(2):59;
[3]Mohammed,M.J.;Marston,C.K.;Popovic,Tanja.;Weyant,Robbin S.;Tenover,Fred C..Antimicrobial Susceptibility Testing of Bacillus anthracis:Comparison of Results Obtained by Using the National Committee for Clinical Laboratory Standards Broth Microdilution Reference and Etest Agar Gradient Diffusion Methods.J.Clin.Microbiol.2002.40(6):1902-1907;
[4]Zapantis,A.;Lacy,M.K.;Horvat,R.T.;Grauer,D.;Barnes,B.J.;Neal,Brian O'.;Couldry,R..Nationwide Antibiogram Analysis Using NCCLS M39-A Guidelines.J.Clin.Microbiol.2005.43(6):2629-2634;
[5]陈林娜,周立勤,王汉敏,谭大奇,李波,刘玲,王春香.中药对临床耐药菌株的体外抑菌试验观察.中华医院感染学杂志.2005.15(1):118-120;
[6]Kiehlbauch,J.A.;Hannett,G.E.;Salfinger,M.;Archinal,W.;Monserrat,C.;Carlyn,C..Use of the National Committee for Clinical Laboratory Standards Guidelines for Disk Diffusion Susceptibility Testing in New York State Laboratories.J.Clin.Microbiol.2000.38(9):3341-3348;
[7]Decross,A.J.;Marshall,B.J.;Mccallum,R.W.;Hoffrnan,S.R.;Barrett,L.J.;Guerrant,R.L..Metronidazole Susceptibility Testing for Helicobacter pylori:Comparison of Disk,Broth,and Agar Dilution Methods and Their Clinical Relevance.J.Clin.Microbiol.1993.31(8): 1971-1974;
[8]索相敏,陆秀君,董建臻,李静,宋萍,赵瑞君,付利燕.中华真地鳖抗菌物质的抑菌活性测定.中国生物防治.2007.23(1):64-67;
[9]Kibsey,P.C.;Rennie,R.P.,Rushton,J.E..Disk Diffusion versus Broth Microdilution Susceptibility Testing of Haemophilus Species and Moraxella catarrhalis Using Seven Oral Antimicrobial Agents:Application of Updated Susceptibility Guidelines of the National Committee for Clinical Laboratory Standards.J.Clin.Microbiol.1994.32(11):2786-2790;
[10]Wanger,A.;Mills,K..Testing of Mycobacterium tuberculosis Susceptibility to Ethambutol,Isoniazid,Rifampin,and Streptomycin by Using Etest.J.Clin.Microbiol.1996.34(7):1672-1676;
[11]Ambaye,A.;Kohner,P.C.;Wollan,P.C.;Roberts,K.L.;Roberts,G D.;Cockerill Ⅲ,F.R..Comparison of Agar Dilution,Broth Microdilution,Disk Diffusion,E-Test,and BACTEC Radiometric Methods for Antimicrobial Susceptibility Testing of Clinical Isolates of the Nocardia asteroides Complex.J.Clin.Microbiol.1997.35(4):847-852;
[12]杨镜明,刘英.异帕米星的体外抗菌活性及临床应用.中国新药与临床.2002.21(1):33;
[13]Brown,W.J..National Committee for Clinical Laboratory Standards Agar Dilution Susceptibility Testing of Anaerobic Gram-Negative Bacteria.Antimicrob.Agents.Chemother.1988.32(3):385-390;
[14]Kirkpatrick,W.R.;Mcatee,R.K.;Revankar,S.G.,Fothergill,A.W.;Mccarthy,D.I.;Rinaldi,M.G.;Patterson,T.F..Comparative Evaluation of National Committee for Clinical Laboratory Standards Broth Macrodilution and Agar Dilution Screening Methods for Testing Fluconazole Susceptibility of Cryptococcus neoformans.J.Clin.Microbiol.1998.36(5):1330-1332;
[15]邹军,李欣欣,杨昭徐,张磊.左氧氟沙星对幽门螺杆菌体外抗菌活性的评价.中国抗生素杂志.2003.28(5):292-294;
[16]Milici,M.E.;Maida,C.M.;Spreghini,E.;Ravazzolo,B.;Oliveri,S.;Scalise,G.;Barchiesi,E.Comparison between Disk Diffusion and Microdilution Methods for Determining Susceptibility of Clinical Fungal Isolates to Caspofungin.J.Clin.Microbiol.2007.45(11):3529-3533;
[17]Pfaller,M.A.;Diekema,D.J.;Procop,G.W.;Rinaldi,M.G..Multicenter Comparison of the VITEK 2 Antifungal Susceptibility Test with the CLSI Broth Microdilution Reference Method for Testing Amphotericin B,Flucytosine,and Voriconazole against Candida spp.J.Clin.Microbiol.2007.45(11):3522-3528;
[18]杜士明,杨宏伟,叶立红.复方左氧氟沙星喷雾剂的体外抑菌实验.中国医院药学杂志.2007.27(3):418-420;
[19]Pfaller,M.A.;Messer,S.A.;Mills,K.;et al..In vivo susceptibility testing of Filamentous fungi:comparison of Etest and reference microdilution methods for determining itroconazole MICs.J.Clin.Microbiol.2000.38(9):3359-3361;
[20]徐英春,谢秀丽,王澎,张悦贤,原英,张小江,朱任媛,刘娟,王辉,陈民钧.Etest法测定伊曲康唑对曲霉和酵母菌的体外抗菌活性.中国新药杂志.2004.13(2):163-165;
[21]Clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing M100-S16[S].Villanova Pa:CLSI/NCCLS.2006.1;
[22]白群华,肖永红,姚成,余泽波,陈海.加替沙星体外抗菌活性研究.中国抗生素杂志.2002.27(10):621-629;
[23]徐雅萍,罗燕萍,白立彦,张巧云.加替沙星与4种喹诺酮类药物体外抗菌活性比较.中华医院感染学杂志.2005.15(1):76-78;
[24]赵水娣,梅亚宁.美罗培南等7种抗菌药物对300株革兰阴性杆菌体外抗菌活性比较.临床检验杂志.2007.25(5):383-384;
[25]李仲兴,王秀华,孟晓洁,李玮,杨敬芳,时东彦,王鑫.莫西沙星对224株凝固酶阴性葡萄球菌的体外抗菌活性观察.中国抗生素杂志.2005.30(8):498-502;
[26]蒋玉富,邹启园,胡鹏,王其南,李崇智,郑行萍,刘鸿渝,陈海.乳酸左氧氟沙星体外抗菌作用研究.中国抗生素杂志.2000.25(1):47-50;
[27]王丽平,江善祥,史晓丽,陈绍峰.替米考星的体外抑菌试验和其磷酸盐预混剂对鸡慢性呼吸道病的疗效试验.中国兽医科技.2003.33(9):55-58;
[28]王付民,胡功政,苑丽,张春辉,梁军,杨玉荣.头孢噻呋等的体外抗菌活性研究.中国兽医杂志.2004.40(9):49-50;
[29]刘明春,何剑斌,于立辉,张文奎,曹峰,沈建忠.头孢噻肟钠体外抗菌活性及对实验性感染鸡大肠杆菌病的药效学试验.中国兽医杂志.2007.43(10):61-63;
[30]杜平华,朱世真,陈涛.胃灵丹对幽门螺杆菌体外抑菌试验.药物分析杂志.2000.20(1):21-22;
[31]霍春芳,张冬艳,刘进荣,张通.稀土对芽孢菌的抑菌机理研究.化学学报.2002.60(6):1065-1071;
[32]李聪然,游雪甫,张伟新,娄人慧,杨信恰,王跃明,陈慧贞,蒋建东.新氨基糖苷类抗生素vertimicin的体内抗菌活性研究.中国抗生素杂志.2007.32(6):357-361;
[33]何秀琴,周远大.盐酸左氧氟沙星体外抗菌活性的研究.中国药业.2001.10(7):33-34;
[34]董华,任士荣.氧氟沙星锌的制备及其体外抑菌试验.中国医院药学杂志.2000.20(12):759-760;
[35]张芳,李玉敏,王立秋,崔琴.异帕米星对金葡菌的体外抗菌活性分析.中国抗生素杂志.2007.32(6):365-366;
[36]李淑梅,杨帆.中草药提取物对大肠杆菌的体外抑菌试验.安徽农业科学.2006.34(23):6212, 6222;
[37]National Committee for Clinical Laboratory Standards(NCCLS).Performance Standards for Antimicrobial Susceptibility Testing;Sixteenth Informational Supplement.NCCLS document.M100-S16.2006.26(3);
[38]Carson,C.F.,Hammer,K.A.,Piley,T.V..Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Melaleuca altemifolia(tea tree oil).Microbios.1995.82(332):181-185;
[39]Ordonez,J.V.,Wehman,N.M..Rapid Flow Cytometric Antibiotic Susceptibility Assay for Staphylococcus aureus.Cytometry.1993.14:811-818;
[40]张致平.微生物药物学.第一版.北京.化学工业出版社.2003.196-208;
[41]Zhang,M.G.,Gorski,W..Electrochemical Sensing Based on Redox Mediation at Carbon Nanotubes.Anal.Chem..2005.77(13):3960-3965;
[1]Boman,H.G..Antibacterial Peptides:key components needed in immunity.Cell.1991.65:205-207;
[2]Campos,M.A.,Morey,P.,Bengoechea,J.A..Quinolones Sensitize Gram-Negative Bacteria to Antimicrobial Peptides.Antimicrob.Agents.Chemother..2006.50(7):2361-2367;
[3]Jenssen,H.,Hamill,P.,Hancock,R.E.W..Peptide Antirnicrobial Agents.Clin.Microbiol.Rev.2006.19(3):491-511;
[4]许若丹,钟理,张伟,柳伟强,牛建章.阳离子抗菌肽结构与抗菌活性的关系.中国公共卫生.2005.21(9):1141-1143;
[5]易祥华,吴克兰,刘绍春,赵时宇,胡建平.朊病毒蛋白在胶质瘤中表达的研究.同济医科大学学报.1997.26(3):184-190;
[6]Simone,A.D.,Dodson,G.G.,Fratemali,F.,Zagari,A..Water molecules as structural determinants among prions of low sequence identity.FEBS Lett..2006.580.2488-2494;
[7]Martins,V.R.,Linden,L.,Prado,M.A.,Walz,R.,Sakamoto,A.C.,Izquierdo,I.and Brentani,R.R..Cellular prion protein:on the road for functions..FEBS Lett..2002.512:25-28;
[8]Westergard,Laura.,Christensen,H.M.,Harris,D.A..The Cellular Prion Protein(PRP~c):Its Physiological Function And Role In Disease.Biochim.Biophys.Acta..2007.1772:629-644;
[9]Wieprecht T.,Apostolov O.,Beyermann M.,et al.Membrane binding and pore formation of the antibacterial peptide PGLa:thermodynamic and mechanistic aspects,Biochemistry[J],2000,39(2):442-452;
[10]Hancock R.E.W.,Rozek A.,Role of membranes in the activities of antimicrobial cationic peptides.FEMS Microbiol Lett[J],2002,206(2):143-149;
[11]Patrzykat A.,Friedrich C.L.,Zhang L.,et al.Sublethal concentrations of pleurocidin-derived antimicrobial peptides inhibit macromolecular synthesis in Escherichia coli.Antimicrob Agents Chemother[J],2002,46(3):605-614;
[12]Zhang L.,Rozek A.,Hancock R.E.W.,Interaction of cationic antimicrobial peptides with model membranes.Biol Chem[J],2001,276(38):35714-35722;
[13]Friedrich C.L.,Moyles D.,Beveridge T.J.,et al.Antibacterial action of structurally diverse cationic peptides on Gram-positive bacteria.Antimicrob Agents Chemother[J],2000,44(8):2086-2092;
[2]张亚梅.药物中毒性耳聋.中华儿科杂志.2000.12,38(12):781-782.北京.中华医学会;
[3]Cuzzocrea,S.;Mazzon,E.;Dugo,L.;et al.A role for superoxide in gentamicin-mediated nephropathy in rats.Eur J Pharmacol.2002.450(1):67-76;
[4]Wallace,K.B..Doxorubicin induced cardiac mitochondrionopathy.Pharmacol Toxicol.2003.93:105-115;
[5]Galliteni,M.;Braidotli,P.;Cozzolino,M.;et al.Acute Tubulo-interstitial nephritis requiring dialysis with intermittent rifampcin use:case report.Int J Antif Organs.1999.22(7):477-479;
[6]Ravi,D.;Das,K.C..Redox-cycling of anthracyclines by thioredoxin system:increased superoxide generation and DNA damage.Cancer Chemother Pharmacol.2004.54(5):449-458;
[7]Ali,B.H..Agents ameliorating or augmenting experimental gentamicin nephrot- oxicity:some recent research.Food Chem Toxicol.2003.41(11):1447-1452;
[8]Sakac,V.;Sakac,M..Free oxygen radicals and kidney diseases - part Ⅰ.Med Pregl.2000.53(9-10):463-474;
[9]Liu,F.T.;Kelsey,S.M.;Newland,A.C.;et al..Liposomal encapsulation diminishes daunorubicin-induced generation of reactive oxygen species,depletion of ATP and necrotic cell death in human leukaemic cells.Br J Haematol.2002.117(2):333-342;
[10]Covic,A.;Goldsmith,D.J.;Segall,L.;et al..Rifampicin-induced acute renal failure:a series of 60 patients.Nephrol Dial Transplant.1998.13(4):924-929;
[11]Popa,R.;Anniko,M.;Takumida,M.;et al.Localization of nitric oxide synthase isoforms in the human cochlea.Acta Otolaryngol.2001.121(4):454-459;
[12]吾建浓,施敏凤,王灵聪,叶雪惠.万古霉素治疗葡萄球菌肺炎的疗效与肾毒性评价.中国新药与临床杂志.2005.24(3):245-247;
[13]Childs,A.C.;Phaneuf,S.L.;Dirks,A.J.,et al.Doxorubicin treatment in Vivo causes cytochrome c release and cardiomyocyte apoptosis,as well as increased mitochondrial efficiency,superoxide dismutase activity,and bcl-2:bax ratio.Cancer Res.2002.62:4592-4598;
[14]Zhou,Y.;Hileman,E.O.;Plunkett,W.;et al..Free radical stress in chronic lymphocytic leukemia cells and its role in cellular sensitivity to ROS-generating anticancer agents.Blood. 2003.101(10):4098-4104;
[15]Nelson,E.B..Kidney failure and analgesic drugs.New Engl J Med.1995.332(22):1514-1516;
[16]Hattori,S.;Yosioka,K.;Honda,M.;e t al..The 1998 report of the Japanese national registry data on pediatric end-stage renal disease patients.Pediatr Nephrol.2002.17(6):456-461;
[17]Yi,J.;Gao,F.;Shi,G.;et al..The inherent cellular level of reactive oxygen species:one of the mechanisms determining apoptotic susceptibility of leukemic cells to arsenic trioxide.Apoptosis.2002.7(3):209-215;
[18]臧宏科,徐冬梅.头孢菌素类抗生素的肾毒害及护理.山东医药.2003.43(2):66;
[19]Sone,M.;Schachern,P.A.;Paparella M.M.;Paparella,M.M..Loss of spiral ganglion cells as primary manifestation of aminoglycoside ototoxicity.Hear Res.1998.115:217-223;
[20]Conlon,B.J.,Smith,D.W..Attenuition of neomycin ototoxicity by iron chelation.Laryngoscope.1998.108(2):284;
[21]Struelens,M.J.,Byl,B.,Vinecnt,J.L..Antibiotic policyl a tool for controlling resistance of hospital pathogens.Clin.Microbiol.Infect..1995.5:519-524;
[22]Khan,S.A.;Nawaz,M.S.;Khan,A.A.;Cerniglia C.E..Transfer of Erythromycin Resistance from Poultry to Human Clinical Strains of Staphylococcus aureus.J.Clin.Microbiol..2000.38(5):1832-1838;
[23]Shannon,K.P.;French,G.L..Antibiotic resistance:effect of different criteria for classifying isolates as duplicates on apparent resistance frequencies.J.Antimicrob.Chemother..2002.49:201-204;
[24]Eavcs,D.J.;Randall,L.;Gray,D.T.;et al.Prevalence of mutations with the quinolone resistance,determining region of gyrA,gyrB,par C,and par E and association with antibiotic resistance in quinolone-resistant Salmonella enterica.Antimicrob.Agents.Chemother..2001.48:4012-4015;
[25]Wise,R..Antimicrobial resistance:paradox,actions and economics.J.Antimicrob.Chemother..2006.57:1024-1025;
[26]Gholizadeh,Y.;Couvalin,P..Acquired and intrinsic glycopeptide resistance in enterococci.Intemational J of Antimicrobial Agents.2000.16:511-517;
[27]Lin,S.Y.G.;Probert,W.;Lo,M.;Desmond,E..Rapid Detection of Isoniazid and Rifampin Resistance Mutations in Mycobacterium tuberculosis Complex from Cultures or Smear-Positive Sputa by Use of Molecular Beacons.J.Clin.Microbiol.2004.42(9):4204-4208;
[28]Katayama,J.;Okada,H.;O' Hara,K.;et al.Isolation and characterization of two plasmids that meliate macrolide resisfance in Escherichia coli:transferability and molecular properties.Biol PharmBull.1998.21:326;
[29]Lee,S.O.;Cho,Y.K.;Kim,S.Y.;Lee,E.S.;Park,S.Y.;Seo,Y.H..Comparison of Trends of Resistance Rates over 3 Years Calculated from Results for All Isolates and for the First Isolate of a Given Species from a Patient.J.Clin.Microbiol.2004.42(10):4776-4779;
[30]Leclercq,R.;Courvalia,P..Bacterial resistance to macrolide,lincosamide and streptogramin antibiotics bytarget modification.Antimicrob.Agents.Chemother..1991.35:1267-1272;
[31]Magee,J.T..Effects of duplicate and-screening isolates on surveillance of community and hospital antibiotic resistance.J.Antimicrob.Chemother..2004.54:155-162;
[32]Leclercq,R.;Dutka-Malen,S.;Brissor-Noel,A.;et al..Resistance of entercocci to aminoglycosides and glycopeptides.Clin infect Dis.1992.15:415-501;
[33]Du,Z.M.;Yang,R.E;Guo,Z.B.;Song,Y.J.;Wang,J..Identification of Staphylococcus aureus and Determination of Its Methicillin Resistance by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.Anal.Chem..2002.74:5487-5491;
[34]Llano-Sotelo,B.;Azucena,EFJr.;Kotra,L.P.;et al..Aminoglycosides modified by resistance enzymes display diminished binding to the bacterial riibosomal aminoacyl-tRNA site.Chem Biol.2002.9:455-501;
[35]赵亮,陈凌,陈贻锴.白色假丝酵母菌耐药性与耐药基因表达及蛋白酶的关系.临床检验杂志.2007.25(5):348-350;
[36]彭敬红,孙自鳙,周有利,吕军.产ESBLs大肠埃希菌的耐药性研究.现代预防医学.2007.34(19):3668-3672;
[37]贾杰.抗生素的耐药性与抗生素的应用.中国热带医学.2007.7(9):1678-1680;
[38]Emmanuelle,C.;Elaine,T..Mechanisms of antibiotic resisitence and tolerance in Streptococcus pneumoniae.Microbes and Infection.2000.2:1855-1864;
[39]杨斌爱,武改娣,马淑玲,呼萍萍.葡萄球菌的分布及耐药性分析.山西医药杂志.2007.36(9):858-859;
[40]万同己.幽门螺杆菌根除治疗的失败原因及其防治对策.中国热带医学.2007.7(9):1590-1592;
[41]Liao,Q.G.,Li,Y.F.,Huang,C.Z..A light scattering and fluorescence emission coupled ratiometry using the interaction of functional CdS quantum dots with aminoglycoside antibiotics as a model system.Talanta.2007.71:567-572;
[42]Huang,C.Z.,Li,Y.F.,Feng,P..A spectrophotometric study on the interaction of neutral red with double-stranded DNA in large excess.Anal.Chim Acta.2001.55:321-328;
[43]Long,Y.J.,Li,Y.F.,Huang,C.Z..A wide dynamic range detection of biopolymer medicines with resonance light scattering and absorption ratiometry.Anal.Chim.Acta..2005.552: 175-181;
[44]Huang,C.Z.,Li,Y.F.,Huang,X.H.,Li,M..Interactions of Janus Green B with double stranded DNA and the determination of DNA based on the measurement of enhanced resonance light scatteringAnalyst.2000.125:1267-1272;
[45]Long,Y.F.,Huang,C.Z..Spectral studies on the interaction of Amido black 10B with DNA in the presence of cetyltrimethylammonium bromide.Talanta.2007.71:1939-1943;
[46]易平贵,刘俊峰,商志才,俞庆森.荧光光谱法研究亚甲基蓝与蛋白质的结合反应.光谱学与光谱分析.2001.21(6):826-828;
[47]肖厚荣,盛良全,施春华,徐小龙,解永树,刘清亮.水杨酸与牛血清蛋白相互作用的荧光光谱研究.光谱学与光谱分析.2004.24(1):78-81;
[48]李改仙,李建晴,魏玉霞,董川.四碘荧光素与牛血清白蛋白相互作用的荧光光谱研究.光谱学与光谱分析.2005.25(8):1277-1280;
[49]颜承农,童金强,熊丹,刘义,潘祖亭.荧光光谱法研究培氟沙星与牛血清白蛋白结合反应特征.分析化学.2006.34(6):796-800;
[50]陈晓波,康栋国,李崧,赵承易,陈晓珊,丁焕平.利福平与人血清白蛋白作用的荧光光谱.光谱学与光谱分析.2006.26(4):674-677;
[51]林伟,彭亦如,陈奎治,翁家宝,徐国兴.光谱分析法研究四磺酸酞菁金属配合物与牛血清白蛋白的结合作用.分析化学.2006.34(3):411-414;
[52]马国欣,王成龙,范多旺,邢达,钱隆,王锦辉,刘颂豪.红外光谱法测定农药中吡虫啉含量.光谱学与光谱分析.2006.26(3):434-437;
[53]李占龙,周密,左剑,王云鹏,姜永恒,里佐威.红外光谱和拉曼光谱法分析玉米种子的成分.分析化学.2007.35(11):1636-1638;
[54]陈奎治,彭亦如,林伟,曹育红,徐国兴,翁家宝.光谱分析法研究八羧酸酞菁铝配合物与牛血清白蛋白的结合作用.光谱学与光谱分析.2007.27(1):84-87;
[55]李燕,吴然然,于佰华,王俊德.红外光谱在中药定性定量分析中的应用.光谱学与光谱分析.2006.26(10):1846-1849;
[56]武汉大学化学系主编.仪器分析.第一版.北京:高等教育出版社.2001.11-36;
[57]李原芳,黄承志,胡小莉.共振光散射技术的原理及其在生化研究和分析中的应用.分析化学.1998.26(12):1508-1515;
[58]梁柱.光学原理教程.第一版.北京:北京航空航天大学出版社.2005.65-163;
[59]R.Pecora.Dynamic Light Scattering.Plenum Press.New York.1985;
[60]左榘.激光散射原理及在高分子科学中的应用.第一版.郑州.河南科学技术出版社.1994.40-259;
[61]杨文治.物理化学实验技术.第一版.北京:北京大学出版社.1992.317-327;
[62]Huang,C.Z.;Li,Y.F..Resonance light scattering technique used for biochemical and pharmaceutical analysis.Anal.Chim.Acta.2003.500.105-117;
[63]Pastemack,R.F.;Bustamante,C.;Collings,P.J.;Giannetto,A.;Gibbs,E.J..Porphyrin Assemblies on DNA as Studied by a Resonance Light-Scattering Technique.J.Am.Chem.Soc..1993.115(13):5393-5399;
[64]黄剑平,梅平,陈芳,何治柯.Ru(bpy)_2 PIP(Ⅳ)~(2+)与DNA作用的共振光散射光谱分析及应用.武汉大学学报(物理版).2007.53(4):426-430;
[65]万新军,刘金水,王伦.吖啶橙共振光散射法测定脱氧核糖核酸.光谱学与光谱分析.2005.25(5):754-756.
[66]冯硕,李正平,张淑红,方正.阿霉素与DNA作用的共振光散射研究及在DNA分析中的应用.光谱学与光谱分析.2006.26(4):707-710;
[67]Huang,C.Z.;Li,Y.F.;Deng,S.Y.;Liu,S.R..Assembly of Methylene Blue on Nucleic Acid Template as Studied by Resonance Light-Scattering Technique and Determination of Nucleic Acids of Nanogram.Bull.Chem.Soc.Jpn..1999.72.1501-1508;
[68]冯宁川,林枫,刘利军,刘建平.次甲基绿.溴代十六烷基三甲基铵体系共振光散射法测定脱氧核糖核酸.中国卫生检验杂志.2005.15(2):172-173;
[69]Li,Y.F.;Shu,W.Q.;Feng,P.;Huang,C.Z.;Li,M..Determination of DNA with Cetyltrimethylammonium Bromide by the Measurement of Resonance Light Scattering.Anal.Sci..2001.17:693-696;
[70]李波,于京华,谭云,程晓亮,朱元娜.共振瑞利散射法测定核酸探针的发展及应用.济南大学学报(自然科学版).2007.21(3):210-214;
[71]曾金祥,陈小明,蔡昌群,罗和安.碱性品红共振光散射法测定DNA研究.分析科学学.报.2005.21(6):664-666;
[72]陈展光,丁卫锋,韩雅莉,卢峰,廖栩泓,陈小明.两性离子表面活性剂作为共振光散射探针测定脱氧核糖核酸.分析化学.2005.33(4):519-522;
[73]冯硕,李正平,吴秋华,方正,张淑红.以多胺为探针的DNA共振光散射光谱法测定.理化检验-化学分册.2007.43(7):555-557;
[74]Li,Y.F.;Huang,C.Z.;Huang,X.H.;Li,M..Determination of DNA by its enhancement effect of resonance light scattering by azur A.Anal.Chim.Acta..2001.429:311-319;
[75]Huang,C.Z.;Li,K.A.;Tong,S.Y..Determination of Nanograms of Nucleic Acids by Their Enhancement Effect on the Resonance Light Scattering of the Cobalt(Ⅱ)/4-[(5-Chloro-2-pyridyl)azo]-1,3-diaminobenzene Complex.Anal.Chem..1997.69:514-520;
[76]Huang,C.Z.;Li,Y.F.;Liu,X.D..Determination of nucleic acids at nanogram levels with safranine T by a resonance light-scattering technique.Anal:Chim.Acta..1998.375:89-97;
[77]Huang,C.Z.;Li,K.A.;Tong,S.Y..Determination of Nucleic Acids by a Resonance Light-Scattering Technique with α,β,γ,δ-Tetrakis[4-(trimethylammoniumyl)phenyl]porphine.Anal.Chem..1996.68:2259-2263;
[78]Long,Y.F.;Huang,C.Z.;Li,Y.F..Hybridization Detection of DNA by Measuring Organic Small Molecule Amplified Resonance Light Scattering Signals.J.Phys.Chem.B..2007.111(17):4535-4538;
[79]Li,Y.F.;Huang,C.Z.;Huang,X.H.;Li,M..Determination of Proteins Based on Their Resonance Light Scattering Enhancement Effect on Alcian Blue 8GX.Anal.Sci..2000.16:1249-1254;
[80]陈红旗,马娟,刁雪莲,王伦.PbS-PAA复合纳米粒子共振光散射法测定蛋白质.分析测试学报.2005.24(6):56-58;
[81]干宁,候琳熙,,李天华,徐伟民,周雄.共振瑞利散射测定痕量甲胎蛋白含量研究.2007.29(2):73-75;
[82]陈丹丹,张涛,任丽萍,石登荣,于金莲.光谱法研究钙红-Cu(Ⅱ)络合物与蛋白质的相互作用及其应用.光谱学与光谱分析.2006.26(4):686-689;
[83]杨新玲,王恩波,刘霞,吴惠毅.丽春红S共振光散射光谱法测定尿蛋白.临床检验杂志.2007.25(3):180-181;
[84]汪新,刘金水,杨小红,王伦.茜素黄GG共振光谱散射法测定人血清白蛋白.光谱实验室.2006.23(2):393-397;
[85]林旭聪,吴孟辉,郭良洽,谢增鸿.新的光散射体系测定蛋白质的研究.光谱学与光谱分析.2007.27(8):1587-1590;
[86]栾吉梅,张晓东.有机染料共振光散射法测定蛋白质的研究进展.理化检验-化学分册.2007.43(2):159-163;
[87]Li,Y.F.;Huang,C.Z.;Li,M..Anal.Sci..A Resonance Light-Scattering Determination of Proteins with Fast Green FCF.2002.18:177-181;
[88]张爱梅,刘妮娜.靛蓝胭脂红共振瑞利散射法测定蛋白质.分析科学学报.2006.22(3):327-329;
[89]Yang,C.X.;Li,Y.F.;Huang,C.Z..Anal.Sci..Determination of Protein with Fast Red VR by a Corrected Resonance Light-Scattering Technique.2003.19:211-215;
[90]王守兴,王峰,黄薇.共振光散射测定蛋白质的新方法.分析科学学报.2007.23(4):464-466;
[91]曾铭,李树伟,王小红.共振散射光谱法研究人血清白蛋白与二甲酚橙的反应.理化检验-化学分册.2007.43(2):93-101;
[92]邵辉莹,张爱梅.甲基蓝体系共振瑞利散射法测定蛋白质.食品科学.2006.27(3):199-201;
[93]Huang,C.Z.;Li,Y.F.;Mao,J.G.;Tan,D.G..Determination of protein concentration by enhancement of the preresonance light-scattering of α,β,γ,δ-tetrakis(5-sulfothienyl)porphine.Analyst.1998.123:1401-1406;
[94]Huang,C.Z.;Li,Y.F.;Feng,P..Determination of proteins with α,β,γ,δ-tetrakis(4-sulfophenyl)porphine by measuring the enhanced resonance light scattering at the air/liquid interface.Anal.Chim.Acta..2001.443:73-80;
[95]Huang,C.Z.;Liu,Y.;Wang,Y.H.;Guo,H.P..Resonance light scattering imaging detection of proteins with α,β,γ,δ-tetrakis(p-sulfophenyl)porphyrin.Anal.Biochem..2003.321:236-243;
[96]Huang,C.Z.;Pang,X.B.;Li,Y.F.;Long,Y.J..A resonance light scattering ratiometry applied for binding study of organic small molecules with biopolymer.Talanta.2006.69:180-186;
[97]贾桂芳,王秋霞,孙英,周志强.吡虫啉与脱氧核糖核酸相互作用机理及其影响因素环境化学.2005.24(4):430-433;
[98]Feng,P.;Shu,W.Q.;Huang,C.Z.;Li,Y.F..Total Internal Reflected Resonance Light Scattering Determination of Chlortetracycline in Body Fluid with the Complex Cation of Chlortetracycline-Europium-Trioctyl Phosphine Oxide at the Water/Tetrachloromethane Interface.Anal.Chem..2001.73.4307-4312;
[99]吴呈珂,冯素玲,樊静.光谱法研究盐酸多塞平与固绿的相互作用及其分析应用.光谱学与光谱分析.2007.27(12):2490-2493;
[100]曾铭,李树伟,邓嘉利,李小燕,赵可清.槲皮素的共振光散射光谱研究光谱学与光谱分析.2006.26(2):317-320;
[101]衷明华.抗坏血酸的共振光散射法测定.食品科学.2008.29(1):239-240;
[102]Wu,L.P.;Li,Y.F.;Huang,C.Z.;Zhang,Q..Visual Detection of Sudan Dyes Based on the Plasmon Resonance Light Scattering Signals of Silver Nanoparticles.Anal.Chem..2006.78:5570-5577;
[103]黄明元,彭晓君,李延志.共振光散射法检测辣椒及其制品中苏丹红Ⅰ.现代预防医学.2007.34(3):528-529;
[104]杨胜园,吕昌银,陆松,于军晖,杨慧仙.苏丹红Ⅰ-甲醇体系的共振光散射光谱研究及其应用.分析科学学报.2007.23(6):739-740;
[105]韩丽娟,李贵荣,谭广辉,杨慧仙,贺冬秀,于军辉,王志湘.共振光散射法测定痕量甲醛.中国卫生检验杂志.2006.16(2):189-190;
[106]李娜,刘玮炜,周洪英,周丽华,赵跃强.共振散射法测定中草药中的微量铬.分析实验室.2007.26(6):85-87;
[107]陈兰化,刘发光.共振散射光谱法测定水中铬(Ⅵ).中国卫生检验杂志.2006.16(2):205-206;
[108]杨静,廖力夫,杨江柳,袁金涛,刘传湘.光化学催化动力学共振光散射法测定痕量铜.分析实验室.2007.26(3):110-113;
[109]贺冬秀,李贵荣,陈运生,刘运美.健那绿共振光散射法测定水样中痕量铬(Ⅵ).分析科学学报.2006.22(3):336-338;
[110]谢济运,蒋治良,钟福新,冯忠伟,郭秀姣.血细胞的光谱特性研究.分析测试技术与仪器.2000.6(3):178-181;
[111]彭一兵,谢济运,蒋治良.人红细胞的共振散射光谱分析.广西科学.2001.8(3):197-198;
[112]谢济运,蒋治良.柑桔溃疡菌的共振散射光谱.物理化学学报.2001.17(5):406-411;
[113]杨明媚,蒋治良.兔红细胞的共振散射光谱研究.光谱学与光谱分析.2002.22(2):204-208;
[114]廖祖荷,罗杨合,蒋治良,谢济运.大肠杆菌的共振散射光谱研究.分析测试技术与仪器.2003.9(2):65-69;
[115]陈绍芬,黄承志,谭克俊.光散射法快速灵敏测定和表征啤酒酵母.高等学校化学学报.2006.27(6):1-5;
[116]左连富.流式细胞术与生物医学.第一版.沈阳:辽宁科学技术出版社.1996.1;
[117]王书奎,周振英.实用流式细胞术彩色图谱.第一版.上海:第二军医大学出版社.2004.2-3;
[118]A' LVAREZ-BARRIENTOS,A.,ARROYO,J.,CANTO' N,R.,NOMBELA,C.,SA'NCHEZ-PE'REZ,M..Applications of Flow Cytometry to Clinical Microbiology.CLIN.MICROBIOL.REV.2000.13(2):167-195;
[119]宝福凯.流式细胞术在免疫学中的应用.广东科技.1996.(9):5-6;
[120]Tsumagari,S.;Otani,I.;Tanemura,K.;Namba,S.;Ohtaki,T.;Kamata,H.;Kanayama,K..Characterization of CD34+ Cells from Canine Umbilical Cord Blood,Bone Marrow Leukocytes,and Peripheral Blood by Flow Cytometric Analysis.J.Vet.Med.Sci.2007.69(11):1207-1209;
[121]李成海,鲁心安,高锋,胡雷光.用流式细胞仪监测放疗肿瘤患者淋巴细胞亚群.中华放射医学与防护杂志.2007.27(3):255-256;
[122]周克元,张月飞,蔡康荣,罗国庆,张群洲,凌光鑫.流式细胞仪检测52例鼻咽癌外周血T淋巴细胞亚群.中国实验诊断学.1997.1(1):38-39;
[123]赵宏丽,孙晓慧,赵俊芳,李孟娟,丁素光.流式细胞仪对系统性红斑狼疮患者CD~(8+)T 淋巴细胞亚群的检测.中国中西医结合皮肤性病学杂志.2005.4(1):7-9;
[124]杨春莉,裘宇容.流式细胞仪对系统性红斑狼疮患者T淋巴细胞亚群及NK细胞的检测.第一军医大学学报.2000.20(3):274-275;
[125]郭华章,殷缨,刘建,胡蕴玉,张盈华.流式细胞仪检测重组合异种骨移植患者外周血T 淋巴细胞亚群的变化.第四军医大学学报.1998.19(5):537-538;
[126]钱叶勇,李炎唐,敖建华,李岩.流式细胞仪检测肾移植后淋巴细胞亚群的临床意义.临 床泌尿外科杂志.1996.11(4):240-242;
[127]吴福国,邱翠娥,韩晓琳,朱立平,蒋明.类风湿性关节炎患者2H~(4+)T淋巴细胞亚群的流式细胞仪分析.中国免疫学杂志.1999.15(3):132-134;
[128]Kandathil,A.J.;Kannangai,R.;David,S.;Nithyanandam,G.;Solomon,S.;Balakrishnan,P.;Abraham,O.C.;Subramanian,S.;Rupali,P.;Verghese,V.P.;Pulimood,S.;Sridharan,G..Comparison of Microcapillary Cytometry Technology and Flow Cytometry for CD4~+and CD8~+T-Cell Estimation.Clin.Diagn.Lab.Immunol..2005.12(8):1006-1009;
[129]王甫厚,陈绍先.用流式细胞仪测定细胞毒.中国实验临床免疫学杂志.1991.3(1):45-50;
[130]Garcia,J.A.;Monteserin,M.C.;Delgado,I.;et al..Aberrant immunophenotypes detected by flow cytometry in acute lymphoblastic leukemia..Leuk.Lymphoma.2000.36:275-284;
[131]Ito,S.;Ishida,Y.;Murai,K.;et al..Flow cytometric analysis of aberrant antigen expression of blasts using CD45 blast gating for minimal residual disease in acute leukemia and high-risk myelodys-plastic syndrome.Leuk.Res..2001.25(3):205-211;
[132]Stelzer,G.T.;Shults,K.E.;Loken,M.R..CD45 gating for routine flow cytometric analysis of human bone marrow specimens.Ann NY Acad Sci.1993.677:265-280;
[133]Jennings,C.D.;Foon,K.A..Recent advances in flow cytometry:Application to the diagnosis of hematologic malignancy.Blood.1997.90(8):2863-2892;
[134]赵丽,周兰霞,陆莉,张晓薇.141例白血病三色流式细胞术免疫分型研究.中国免疫学杂志.2005.21(9):717-718;
[135]Borowitz,M.J.;Guenther,K.L.;Shults,K.E.;et al..Immunophenotyping of acute leukemia by flow cytometric analysis use of CD45 and right-angle light scatter to gate on leukemic blasts in three-color-analysis.Am J Clin Pathol.1993.100:534-540;
[135]Rogelio,P.A.;Lina,R.G.;Norma,L.S.;et al..Flow cytometric analysis of cell-surface and intracellular antigens in the diagnosis of acute leukemia.Am.J.Hematol..2001.68:69-74;
[136]Browitz,M.J.;Guenther,K.E.;et al..Immunophenotyping of acute leukemia by flow cytometric analysis,Use of CD45 and right-angle light scatter to gate on leukemia blasts in three-color analysis.Am.J.Clin.Pathol..1993.100:534-540;
[137]Drenou,B.;Fardel,O.;Fauchet,R.;et al..Flow cytometry:application for the diagnosis and the follow-up of hematological malignancies.Ann.Biol.Clin..2002.60(6):663-672;
[139]万岁桂,巩文玉,孙雪静,徐娟,田丁.86例儿童急性淋巴细胞白血病三流式细胞术免疫分型研究.中华血液学杂志.2002.23(2):83-86;
[140]张锑,赵玲玲,张春青,徐从高.多参数流式细胞术对外周血白血病免疫分型的研究.细胞与分子免疫学杂志.2004.20(2):225-227;
[141]陈葆国,纪东辉,褚邦勇,张丽,罗文达.多参数流式细胞术鉴别诊断单核细胞相关性白 血病的意义.中国免疫学杂志.2006.22(3):237-240;
[142]胡英萍,徐瑞龙,王香梅,郑昭璟,庄顺红.多色流式细胞术测定急性白血病免疫表型的特征及意义.检验医学.2006.21(5):533-536;
[143]沈红强,汤永民,杨世隆,钱伯芹,宋华,石淑文,徐卫群.多色流式细胞术在222例儿童急性白血病免疫分型中的应用.中华儿科杂志.2003.41(5):334-337;
[144]吴雨洁,李建勇,宋君红,郑文娟,仇海荣,张建富,朱广荣.四色流式细胞术-免疫分型在成人急性髓系白血病诊断中的应用.中华检验医学杂志.2006.29(8):750-752;
[145]Campans,D.;Coustan-Smith,E..Detection of minimal residual disease in acute leukemia by flow cytometry.Cytometry.1999.38:139-152;
[146]刘文生,王随照.微小残留白血病细胞的流式细胞术检测研究.临床检验杂志.2001.19(2):95-96;
[147]邱莲女,周永列,林明,夏晓华,皇甫梅生.流式细胞仪中CD45辅助设门在白血病免疫分型分析中的作用.临床检验杂志.2001.19(1):39-40;
[148]Paredes-Aguilera,R.;Romero-Guzman,L.;Lopez-Santiago,N.;et al..Flow cytometry analysis of cell-surface and intracellular antigens in the diagnosis of acute leukemial.Am.J.Hematol..2001.68:69-74;
[149]王文平,杨进波.流式细胞仪在白血病临床研究中的应用.中国医药导报.2006.3(26):152-153;
[150]Goustan-Smith,E.;Sancho,J.;Behm,F.G.;et al..Prognostic importance of measuring early clearance of leukemic cells by flow cytometry in childhood acute lymphoblastic leukemia.Blood.2002.100:52-58;
[151]Farahat,N.;Morilla,A..Detection of minimal residual disease in B-lineage acute lymphoblastic leukaemia by quantitative flow cytometry.Br.J.Haematol..1998.101:158-164;
[152]赵成艳,高泽斌,马荣,王忠利,何晓琳,姜风全,孙洪军.流式细胞术分析淋巴瘤穿刺液免疫表型的临床意义.中国免疫学杂志.2004.20(3):209-210;
[153]Li,S.;Juco,J.;Mann,K.P.;et al..Flow cytometry in the differential diagnosis of lymphocyte-rich thymoma from p recursor T-cell acute lymphoblastic leukemia/lymphoblastic lymphoma.Am.J.Pathol..2004.121:268-274;
[154]Young,N.A.;Al-Saleem,T.I.;Ehya,H.;et al..Utilization of fine-needle aspiration cytology and flow cytometry in the diagnosis and subclassification of primary and recurrent lymphoma.Cancer.1998.84:252-261;
[155]Gratama,J.W.;Orfao,A..Flow cytometric enumeration of CD34~+ hematopoietic stem and progenitor cells.Cytometry.1998.34:128-142;
[156]Brecher,M.E.;Sims,L.;Schmitz,J.;et al..North American multicenter study on flow cytometric enumeration of CD34~+ hematopoietic stem cells.J.hematotherapy.1996.5:227-236;
[157]Michelson,A.D..Flow cytometric analysis ofplatelets.Vox Sang.2000.78:137-142.
[158]Tomer,A..Platelet activation as a marker for in vivo prothrombotic activity:detection by flow cytometry.J.Biol.Regul.Homeost.Agents..2004.18:172-177;
[159]Michelson,A.D..Flow cytometric assessment of glycoprotein movements in platelets.Platelets.1997.8:105-115;
[160]Garcia-Martinez,M.C.;Labios,M.;Herme-Negildo,C.;et al..The effect of hormone replacement therapy on Ca~(2+)mobilization and P-selectin(CD62P)expression in platelets examined under flow cytometry.Blood.Coagul.Fibrinolysis..2004.15:1-8;
[161]Paul,H.;Allan,H.;Donna,G.;et al..Immunoplatelet counting:a proposed new reference procedure.British Joumal of Haematology.2000.108:228;
[162]Corash,L..Measurement of platelet activatiou by gluoresccnceactivated flow cytometry.Blood cells.1990.16:97;
[163]Shattil,S.J.;Cunningham,M.;Hoxie,J.A.;et al..Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry.Blood.1987.70:307-315;
[164]Jennings,L.K.;Ashmun,R.A.;Wang,W.C.;et al..Analysis of human platelet glycoproteins Ⅱ b-Ⅲa and Glanzamann's thrombasthenin in whole blood by flow cytometry.Blood.1996.68:173-179;
[165]Davies,T.A.;Drotts,D.J.;Weil,C.J.;et al..Flow cytometric measurements of cytoplasmic calcium changes in human platelet.Cytometry.1988.9:138-142;
[166]Johnston,G.L.;Pickeet,E.B.;McEver,R.P.;et al..Heterogeneity of platelet secretion in response thrombin demonstrated by fluorescence flow cytometry.Blood.1987.69:1401-1413;
[167]Ault,K.A..Flow cytometric measurement of platelet function and reticulated platelets.Ann.NY.Acad.Sci..1993.677:293-308;
[168]Gordon,N.;Thorn,J.;Cole,C.;et al..Rapid detection of hereditary and acquired platelet storage pool deficiency by flow cytometry.Br.J.Haematol..1995.89:117-123;
[169]陈军浩,张葵.流式细胞仪计数网织血小板及初步临床应用.临床检验杂志.2001.19(6):337-338;
[170]Michelson,A.D..A clinical test of platelet function.Blood.1996.87:4925-4936;
[171]丁建萍,于琳,申鲁生,王先觉,张俊芳.流式细胞术检测化疗后患儿外周血网织红细胞成熟度的临床意义.临床儿科杂志.2001.19(6):367;
[172]向晓娟,邓承祺,牛挺,雷松,孟文彤.流式细胞术检测网织血小板.中华血液学杂 志.2000.21(9):501;
[173]曹永献,姚远,张飚,于秀英,陈颖,李海霞.流式细胞仪计数网织红细胞方法评价.检验医学.2004.19(3):200-202;
[174]翟中和,王喜中,丁明孝.细胞生物学.第一版.北京:高等教育出版社.2000.358;
[175]Gong,J.;Tragano,E;Darzynkiewicz,Z..Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1.Exp.Cell.Res..1995.220(1):226-231;
[176]谢大兴,吴剑宏,冯永东,李小兰,陶德定,龚建平.细胞周期检测点的流式细胞术分析.癌症.2005.24(9):1151-1155;
[177]徐萌,李金瀚.肺癌耐药发生和细胞周期改变的流式细胞术分析.中华肿瘤杂志.2000.22(5):385-388;
[178]翟中和,王喜中,丁明孝.细胞生物学.第一版.北京:高等教育出版社.2000.454;
[179]Ibuki,Y.;Goto,R..Dysregulation of apoptosis by benzene metabolites and their relationships with carcinogenesis.Biochim.Biophys.Acta..2004.1690.11-21;
[180]Yasuda,N.;Matzno,S.;Iwano,C.;Nishikata,M.;Matsuyama,K..J.Pharm.Biomed.Anal..Evaluation of apoptosis and necrosis induced by statins using fluorescence-enhanced flow cytometry.2005.39:712-717;
[181]Fishman-Lobell,J.;Tsui,P.;Reddy,M.;DiPrinzio,R.;Eichman,C.;Sweet,R.W.;Truneh,A..CD4 mAb induced apoptosis of peripheral T cells:multiparameter subpopulation analysis by flow cytometry using Attractors.J.Immunol.Methods..2001.257:71-82;
[182]David,W.;Hedley,M.D..The Cellular Physiology of Apoptosis Mapped by Multilaser Flow Cytometry.Clin.Immunol.Newslett.1998.18(1/2):1-7;
[183]Michael,G.O..Investigating the relationship between the cell cycle and apoptosis using flow cytometry.J.Immunol.Methods..2002.265:73-80;
[184]冉立伟,谭卫明,谭升顺,王万卷,邱实,刘平.流式细胞仪测量组胺对人角质形成细胞内游离Ca~(2+)的影响.中国皮肤性病学杂志.2005.19(2):75-76;
[185]Scott,W.B.;Bruce,S.E.;Fritz,W.K.;Fredine,T.L.;Eric,R.P.;John,T.R.;Larry,A.S..Analysis of Free Intracellular Calcium by Flow Cytometry:Multiparameter and Pharmacologic Applications.METHODS.2000.21:221-230;
[186]Siegl,E.;Nebe,B.;Blunk,H.;Rychly,J..Detection of mitogen induced stimulation of leukocytes from the rainbow trout(Oncorhynchus mykiss)by flow cytometric analysis of intracellular calcium.Comp.Biochem.Physiol.A.1998.119:915-923;
[187]Adachi,T.;Tsubata,T..FRET-based Ca~(2+)measurement in B lymphocyte by flow cytometry and confocal microscopy.Biochem.Biophys.Res.Commun..2008.367:377-382;
[188]周蓓,黄淑霞,周红霞.流式细胞术在药学研究中的应用.中国医院药学杂志.2006.26(3):326-328;
[189]郑国强,刘叙仪,韩复生,徐光炜.阿霉素耐药性研究与流式细胞分析.中国肺癌杂志.2002.5(1):28-30;
[190]叶传忠,陈仕平,裴雪涛,冯凯,李梁.膀胱癌多药耐药的流式细胞术分析.中华实验外科杂志.2000.17(1):90;
[191]章小平,杨红枚,鲁功成.流式细胞仪在膀胱癌多药耐受研究中的应用.临床泌尿外科杂志.2000.15(9):417-419;
[192]褚忠华,陈涛,王捷,区庆嘉,赵海燕,魏菁,刘建平.体外化疗药敏试验系统联合流式细胞仪在胃肠肿瘤化疗中的应用.中华胃肠外科杂志.2003.6(2):120-123;
[193]尹格平,李强,陈诵芬,孙晓明,吴战英,朱彤宇.流式细胞术检测化疗前后卵巢癌细胞凋亡的临床意义.癌症.2000.19(4):383-386;
[194]Mansour,J.D.,J.A.Robson,C.W.Arndt,and T.H.Schulte.Detection of Escherichia coli in blood using flow cytometry.Cytometry.1985.6:186-190;
[195]Clarke,R.G.,Pinder,A.C..Improved detection of bacteria by flow cytometry using a combination of antibody and viability markers.J.Appl.Microbiol..1998.84:577-584;
[196]Bowden,R.A.,A.Cloeckaert,M.S.Zygrnunt,S.Bernard,and G.Dubray.Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry.Infect.Immun..1995.63:3945-3952;
[197]Cheek,R.F.,I.Olszak,S.Madoff,and F.I.Preffer..In vitro detection of Mycoplasma fermentans binding to B-lymphocytes in fresh peripheral blood using flow cytometry.Cytometry.1997.28:90-95;
[198]Patrick,S.,L.D.Stewart,N.Damani,K.G.Wilson,D.A.Lutton,M.J.Larkin,I.Poxton,and R.Brown..Immunological detection of Bacteroides fragilis in clinical samples.J.Med.Microbiol..1995.43:99-109;
[199]Bhushan,R.,C.Kirkham,S.Sethi,T.F.Murphy..Antigenic characterization and analysis of the human immune response to outer membrane protein E of Branhamella catarrhalis.Infect.Immun..1997.65:2668-2675;
[200]Ozanne,V.,A.Ortalo-Magne,A.Vercellone,J.J.Foumie,and M.Daffe..Cytometric detection of mycobacterial surface antigens:exposure of mannosyl epitopes and of the arabinan segment of arabinomannans.J.Bacteriol..1996.178:7254-7259;
[201]Ingram,M.,T.J.Cleary,B.J.Price,and A.Castro..Rapid detection of Legionella pneumophila by flow cytometry.Cytometry.1982.3:134-147;
[202]Hughes,E.E.,J.M.Matthews-Greer,and H.E.J.Gilleland.Analysis by flow cytometry of surface-exposed epitopes of outer membrane protein F of Pseudomonas aeruginosa.Can.J.Microbiol..1996.42:859-862;
[203]Chaffin,W.L.,Ringler,L.and Larsen,H.S..Interactions of monospecific antisera with cell surface determinants of Candida albicans.Infect.Infect.Immun..1998.56:3294-3296;
[204]Groschel,D.H..Methods,reagents and media:new technology in clinical microbiology.Infect.Control..1983.4:232-233;
[205]Han,Y.,Kanbe,T.,Cherniak,R.,Cutler,J.E..Biochemical characterization of Candida albicans epitopes that can elicit protective and nonprotective antibodies.Infect.Immun..1997.65:4100-4107;
[206]Williams,K.M.,Sacci,J.B.,Anthony,R.L..Characterization and quantitation of membrane antigens of New World Leishmania species by using monoclonal antibodies in Western blot and flow microfluorometric assays.J.Protozool.1986.33:490-497;
[207]Flores,B.M.,Garcia,C.A.,Stamm,W.E.,Torian,B.E..Differentiation of Naegleria fowleri from Acanthamoeba species by using monoclonal antibodies and flow cytometry.J.Clin.Microbiol..1990.28:1999-2005;
[208]Lehman,J.M.,Friedrich,T.D.,Laffm,J..Quantitation of simian virus 40 T-antigen correlated with the cell cycle of permissive and nonpermissive ceils.Cytometry.1993.14:401-410;
[209]周德庆.微生物学教程.第二版.北京:高等教育出版社.2002.3-355;
[1]贾文祥.医学微生物学.第一版.成都:四川大学出版社.2005.103-107;
[2]孙宗科,张伟,陈西平.应用飞行时间质谱仪快速鉴定细菌的初步研究.卫生研究.2004.33(5):552-554;
[3]张晖,林大榕.滤膜荧光法检测大肠菌群及其与标准滤膜法的比较.南京铁道医学院学报.1998.17(1):23-25;
[4]Bartlett M,Huang G,Larcom L,et al.Measurement of particle size distribution in mammalian cells in vitro by use of polarized light spectroscopy.Appl Opt.2004.43(6):1296-1307;
[5]Bronk B V,Druger S D,Czege J,et al.Measuring diameters of rod-shaped bacteria in vivo with polarized light scattering.Biophys.J.1995.69(3):1170-1177;
[6]Bronk B V,Van de Merwe W P,Stanley M.In vivo measure of average bacterial cell size from a polarized light scattering function.Cytometry.1992.13(2):155-162;
[7]Van de Merwe W P,Li Z Z,Bronk B V,et al.Polarized light scattering for rapid observation of bacterial size changes.Biophy.J.1997.73(1):500-506;
[8]Bronk B V,Li Z Z,Czege J.Polarized light scattering as a rapid and sensitive assay for metal toxicity to bacteria.J.Appl.Toxicol.2001.21(2):107-113;
[9]Nicolini C,Diaspro A,Bertolotto M,et al.Changes in DNA superhelical density monitored by polarized light scattering.Biophys.Res.Commun.1991.177(3):1313-1318;
[10]赵小辉,黄承志.蛋白质与十二烷基硫酸钠相互作用的光散射偏振特性.科学通报.2006.51(23):2729-2732;
[11]Feng Hao,Colleen L.Nehl,Jason H.Hafner,et al.Plasmon Resonances of a Gold Nanostar.Nano Lett.2007.7(3):729-732;
[12]Yguerabide,J.;Yguerabide,E.E..Light-Scattering Submicroscopic Particles as Highly Fluorescent Analogs and Their Use as Tracer Labels in Clinical and Biological Applications.Anal Biochem.1998.262:137-156;
[1]韩崧,王淑民,李鑫,闫美玲,柴栋,方翼.4种β-内酰胺类抗生素对临床分离致病菌的体外抗菌活性.中国抗生素杂志.2007.32(6):381-383;
[2]孙民,项明洁,韩立忠,倪语星.11种抗生素对革兰阴性杆菌抗菌活性的比较.中国人兽共患病杂志.2003.19(2):59;
[3]Mohammed,M.J.;Marston,C.K.;Popovic,Tanja.;Weyant,Robbin S.;Tenover,Fred C..Antimicrobial Susceptibility Testing of Bacillus anthracis:Comparison of Results Obtained by Using the National Committee for Clinical Laboratory Standards Broth Microdilution Reference and Etest Agar Gradient Diffusion Methods.J.Clin.Microbiol.2002.40(6):1902-1907;
[4]Zapantis,A.;Lacy,M.K.;Horvat,R.T.;Grauer,D.;Barnes,B.J.;Neal,Brian O'.;Couldry,R..Nationwide Antibiogram Analysis Using NCCLS M39-A Guidelines.J.Clin.Microbiol.2005.43(6):2629-2634;
[5]陈林娜,周立勤,王汉敏,谭大奇,李波,刘玲,王春香.中药对临床耐药菌株的体外抑菌试验观察.中华医院感染学杂志.2005.15(1):118-120;
[6]Kiehlbauch,J.A.;Hannett,G.E.;Salfinger,M.;Archinal,W.;Monserrat,C.;Carlyn,C..Use of the National Committee for Clinical Laboratory Standards Guidelines for Disk Diffusion Susceptibility Testing in New York State Laboratories.J.Clin.Microbiol.2000.38(9):3341-3348;
[7]Decross,A.J.;Marshall,B.J.;Mccallum,R.W.;Hoffrnan,S.R.;Barrett,L.J.;Guerrant,R.L..Metronidazole Susceptibility Testing for Helicobacter pylori:Comparison of Disk,Broth,and Agar Dilution Methods and Their Clinical Relevance.J.Clin.Microbiol.1993.31(8): 1971-1974;
[8]索相敏,陆秀君,董建臻,李静,宋萍,赵瑞君,付利燕.中华真地鳖抗菌物质的抑菌活性测定.中国生物防治.2007.23(1):64-67;
[9]Kibsey,P.C.;Rennie,R.P.,Rushton,J.E..Disk Diffusion versus Broth Microdilution Susceptibility Testing of Haemophilus Species and Moraxella catarrhalis Using Seven Oral Antimicrobial Agents:Application of Updated Susceptibility Guidelines of the National Committee for Clinical Laboratory Standards.J.Clin.Microbiol.1994.32(11):2786-2790;
[10]Wanger,A.;Mills,K..Testing of Mycobacterium tuberculosis Susceptibility to Ethambutol,Isoniazid,Rifampin,and Streptomycin by Using Etest.J.Clin.Microbiol.1996.34(7):1672-1676;
[11]Ambaye,A.;Kohner,P.C.;Wollan,P.C.;Roberts,K.L.;Roberts,G D.;Cockerill Ⅲ,F.R..Comparison of Agar Dilution,Broth Microdilution,Disk Diffusion,E-Test,and BACTEC Radiometric Methods for Antimicrobial Susceptibility Testing of Clinical Isolates of the Nocardia asteroides Complex.J.Clin.Microbiol.1997.35(4):847-852;
[12]杨镜明,刘英.异帕米星的体外抗菌活性及临床应用.中国新药与临床.2002.21(1):33;
[13]Brown,W.J..National Committee for Clinical Laboratory Standards Agar Dilution Susceptibility Testing of Anaerobic Gram-Negative Bacteria.Antimicrob.Agents.Chemother.1988.32(3):385-390;
[14]Kirkpatrick,W.R.;Mcatee,R.K.;Revankar,S.G.,Fothergill,A.W.;Mccarthy,D.I.;Rinaldi,M.G.;Patterson,T.F..Comparative Evaluation of National Committee for Clinical Laboratory Standards Broth Macrodilution and Agar Dilution Screening Methods for Testing Fluconazole Susceptibility of Cryptococcus neoformans.J.Clin.Microbiol.1998.36(5):1330-1332;
[15]邹军,李欣欣,杨昭徐,张磊.左氧氟沙星对幽门螺杆菌体外抗菌活性的评价.中国抗生素杂志.2003.28(5):292-294;
[16]Milici,M.E.;Maida,C.M.;Spreghini,E.;Ravazzolo,B.;Oliveri,S.;Scalise,G.;Barchiesi,E.Comparison between Disk Diffusion and Microdilution Methods for Determining Susceptibility of Clinical Fungal Isolates to Caspofungin.J.Clin.Microbiol.2007.45(11):3529-3533;
[17]Pfaller,M.A.;Diekema,D.J.;Procop,G.W.;Rinaldi,M.G..Multicenter Comparison of the VITEK 2 Antifungal Susceptibility Test with the CLSI Broth Microdilution Reference Method for Testing Amphotericin B,Flucytosine,and Voriconazole against Candida spp.J.Clin.Microbiol.2007.45(11):3522-3528;
[18]杜士明,杨宏伟,叶立红.复方左氧氟沙星喷雾剂的体外抑菌实验.中国医院药学杂志.2007.27(3):418-420;
[19]Pfaller,M.A.;Messer,S.A.;Mills,K.;et al..In vivo susceptibility testing of Filamentous fungi:comparison of Etest and reference microdilution methods for determining itroconazole MICs.J.Clin.Microbiol.2000.38(9):3359-3361;
[20]徐英春,谢秀丽,王澎,张悦贤,原英,张小江,朱任媛,刘娟,王辉,陈民钧.Etest法测定伊曲康唑对曲霉和酵母菌的体外抗菌活性.中国新药杂志.2004.13(2):163-165;
[21]Clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing M100-S16[S].Villanova Pa:CLSI/NCCLS.2006.1;
[22]白群华,肖永红,姚成,余泽波,陈海.加替沙星体外抗菌活性研究.中国抗生素杂志.2002.27(10):621-629;
[23]徐雅萍,罗燕萍,白立彦,张巧云.加替沙星与4种喹诺酮类药物体外抗菌活性比较.中华医院感染学杂志.2005.15(1):76-78;
[24]赵水娣,梅亚宁.美罗培南等7种抗菌药物对300株革兰阴性杆菌体外抗菌活性比较.临床检验杂志.2007.25(5):383-384;
[25]李仲兴,王秀华,孟晓洁,李玮,杨敬芳,时东彦,王鑫.莫西沙星对224株凝固酶阴性葡萄球菌的体外抗菌活性观察.中国抗生素杂志.2005.30(8):498-502;
[26]蒋玉富,邹启园,胡鹏,王其南,李崇智,郑行萍,刘鸿渝,陈海.乳酸左氧氟沙星体外抗菌作用研究.中国抗生素杂志.2000.25(1):47-50;
[27]王丽平,江善祥,史晓丽,陈绍峰.替米考星的体外抑菌试验和其磷酸盐预混剂对鸡慢性呼吸道病的疗效试验.中国兽医科技.2003.33(9):55-58;
[28]王付民,胡功政,苑丽,张春辉,梁军,杨玉荣.头孢噻呋等的体外抗菌活性研究.中国兽医杂志.2004.40(9):49-50;
[29]刘明春,何剑斌,于立辉,张文奎,曹峰,沈建忠.头孢噻肟钠体外抗菌活性及对实验性感染鸡大肠杆菌病的药效学试验.中国兽医杂志.2007.43(10):61-63;
[30]杜平华,朱世真,陈涛.胃灵丹对幽门螺杆菌体外抑菌试验.药物分析杂志.2000.20(1):21-22;
[31]霍春芳,张冬艳,刘进荣,张通.稀土对芽孢菌的抑菌机理研究.化学学报.2002.60(6):1065-1071;
[32]李聪然,游雪甫,张伟新,娄人慧,杨信恰,王跃明,陈慧贞,蒋建东.新氨基糖苷类抗生素vertimicin的体内抗菌活性研究.中国抗生素杂志.2007.32(6):357-361;
[33]何秀琴,周远大.盐酸左氧氟沙星体外抗菌活性的研究.中国药业.2001.10(7):33-34;
[34]董华,任士荣.氧氟沙星锌的制备及其体外抑菌试验.中国医院药学杂志.2000.20(12):759-760;
[35]张芳,李玉敏,王立秋,崔琴.异帕米星对金葡菌的体外抗菌活性分析.中国抗生素杂志.2007.32(6):365-366;
[36]李淑梅,杨帆.中草药提取物对大肠杆菌的体外抑菌试验.安徽农业科学.2006.34(23):6212, 6222;
[37]National Committee for Clinical Laboratory Standards(NCCLS).Performance Standards for Antimicrobial Susceptibility Testing;Sixteenth Informational Supplement.NCCLS document.M100-S16.2006.26(3);
[38]Carson,C.F.,Hammer,K.A.,Piley,T.V..Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Melaleuca altemifolia(tea tree oil).Microbios.1995.82(332):181-185;
[39]Ordonez,J.V.,Wehman,N.M..Rapid Flow Cytometric Antibiotic Susceptibility Assay for Staphylococcus aureus.Cytometry.1993.14:811-818;
[40]张致平.微生物药物学.第一版.北京.化学工业出版社.2003.196-208;
[41]Zhang,M.G.,Gorski,W..Electrochemical Sensing Based on Redox Mediation at Carbon Nanotubes.Anal.Chem..2005.77(13):3960-3965;
[1]Boman,H.G..Antibacterial Peptides:key components needed in immunity.Cell.1991.65:205-207;
[2]Campos,M.A.,Morey,P.,Bengoechea,J.A..Quinolones Sensitize Gram-Negative Bacteria to Antimicrobial Peptides.Antimicrob.Agents.Chemother..2006.50(7):2361-2367;
[3]Jenssen,H.,Hamill,P.,Hancock,R.E.W..Peptide Antirnicrobial Agents.Clin.Microbiol.Rev.2006.19(3):491-511;
[4]许若丹,钟理,张伟,柳伟强,牛建章.阳离子抗菌肽结构与抗菌活性的关系.中国公共卫生.2005.21(9):1141-1143;
[5]易祥华,吴克兰,刘绍春,赵时宇,胡建平.朊病毒蛋白在胶质瘤中表达的研究.同济医科大学学报.1997.26(3):184-190;
[6]Simone,A.D.,Dodson,G.G.,Fratemali,F.,Zagari,A..Water molecules as structural determinants among prions of low sequence identity.FEBS Lett..2006.580.2488-2494;
[7]Martins,V.R.,Linden,L.,Prado,M.A.,Walz,R.,Sakamoto,A.C.,Izquierdo,I.and Brentani,R.R..Cellular prion protein:on the road for functions..FEBS Lett..2002.512:25-28;
[8]Westergard,Laura.,Christensen,H.M.,Harris,D.A..The Cellular Prion Protein(PRP~c):Its Physiological Function And Role In Disease.Biochim.Biophys.Acta..2007.1772:629-644;
[9]Wieprecht T.,Apostolov O.,Beyermann M.,et al.Membrane binding and pore formation of the antibacterial peptide PGLa:thermodynamic and mechanistic aspects,Biochemistry[J],2000,39(2):442-452;
[10]Hancock R.E.W.,Rozek A.,Role of membranes in the activities of antimicrobial cationic peptides.FEMS Microbiol Lett[J],2002,206(2):143-149;
[11]Patrzykat A.,Friedrich C.L.,Zhang L.,et al.Sublethal concentrations of pleurocidin-derived antimicrobial peptides inhibit macromolecular synthesis in Escherichia coli.Antimicrob Agents Chemother[J],2002,46(3):605-614;
[12]Zhang L.,Rozek A.,Hancock R.E.W.,Interaction of cationic antimicrobial peptides with model membranes.Biol Chem[J],2001,276(38):35714-35722;
[13]Friedrich C.L.,Moyles D.,Beveridge T.J.,et al.Antibacterial action of structurally diverse cationic peptides on Gram-positive bacteria.Antimicrob Agents Chemother[J],2000,44(8):2086-2092;