运动对二噁英致大鼠脂质合成代谢障碍的干预作用及其机制研究
摘要
目的：本研究以SD大鼠为研究对象,研究运动、二噁英对大鼠肝脏脂质合成代谢的影响,探讨其影响机制,以此来改变人类对代谢性疾病发病机制的新认识,并为运动锻炼防治二噁英环境污染物引起代谢性疾病提供相应理论支持。方法：选取8周龄雄性SD大鼠随机分为对照组(C)、运动组(E)、染毒组(T)、运动染毒组(ET)。T、ET组大鼠腹腔注射首剂量6.4μg/kg·bw的2,3,7,8-TCDD,之后每隔1w给予上述剂量的21%持续染毒,连续7w。E、ET组大鼠尾部负重(5%bw)进行游泳运动,每周5天,每次30分钟。每日观察大鼠健康状况,给药后第1、3、4、6、8w末内眦静脉取血检测血脂指标,第4、8w末分别处死半数动物,计算脏器相对重量,取肝脏组织行生化指标、组织病理学检测,实时荧光定量PCR、 Western Blot检测ACC1、FAS、SCD1、LXRa、SREBP-1、ChREBP mRNA及蛋白表达。
结果：1、各组大鼠精神状态良好,活动能力正常；体重呈增长趋势,但每组趋势不同；ET、T组肝脏相对重量显著增高；4w末ET组附睾脂肪相对重量显著下降。2、2,3,7,8-TCDD可显著增高肝脏TG含量,长期运动可显著降低肝脏TG含量；各组大鼠中E组血清TG、TC含量最低,ET、T组血清TG、TC呈增长趋势；ET、T组血清LDL-C显著增高,长期运动E组血清HDL-C显著增高。3、肝脏组织HE和油红0染色显示2,3,7,8-TCDD可诱发明显病理性改变及脂质浸润,长期运动可改善病理性改变及脂质浸润情况。4、2,3,7,8-TCDD持续染毒可显著增加脂质合成代谢过程中相关酶ACC1、FAS、SCD1及转录因子LXRa、SREBP-1的mRNA和蛋白表达,长期运动可显著降低染毒大鼠ACC1、FAS、SCD1、LXRa、SREBP-1的mRNA和蛋白表达,2,3,7,8-TCDD染毒和运动对某些指标的影响存在交互作用,表明运动有干预效应。
结论：1、2,3,7,8-TCDD影响肝脏脂质合成代谢过程中相关酶及转录因子的mRNA和蛋白表达,从而造成脂质代谢紊乱。2、运动对肝脏脂质合成代谢的影响具有时间效应,长期运动才明显体现,其具体影响机制还需进一步研究。3、长期运动可改善2,3,7,8-TCDD造成脂质代谢的紊乱。
Objective:This paper investigated the effects of exercise and2,3,7,8-TCDD on the hepatic lipid anabolic in the SD rats, and explored the mechanism of the effects. The aim is to develop a new knowledge of the pathogenesis of metabolic diseases, and provide a corresponding support for the hypothesis that exercise might prevent the metabolic deseases caused by dioxin-like environmental pollutants.
Methods:SD rats (8weeks old) were divided into4different groups randomly:control group (C), exercise group (E), T group treated with2,3,7,8-TCDD, and ET group treated with exercise and2,3,7,8-TCDD. Rats in groups T and ET were intraperitoneally given2,3,7,8-TCDD in a first dose of6.4μg/kg·bw, and thereafter, they were administrated a mantenance dosage by21%of the first dose weekly for7weeks. Groups E and ET swam30min every day with a tail load of5%body weight for5days per week. The healthy status of the rats was daily observed. The blood samples were collected through the inner canthus vein at the end of1st,3rd,4th,6th,8th weekends, and then the serum lipid indicators were measured. Half groups of the rats were killed at the4th and8th weekends respectively, and the relative weights of the organs were calculated. The liver tissues were collected to conduct the biochemical parameter measurements and histopathological detections. ACC1, FAS, SCD1, LXRa, SREBP-1, ChREBP mRNA and protein expression were determined via real-time quantitative PCR and Western Blot methods.
Results:(1) All rats were in good mental state and normal activity. And the body weights showed a growth trend, but there were differences between each group. The relative weights of the livers significantly increased in the rats of groups ET and T. The relative weights of epididymal fat greatly decreased at the4th weekend in the rats of group ET.(2)2,3,7,8-TCDD showed a significantly increasing effect on the TG concentrations in livers, while long-term exercise might have a deceasing effect. Rats in group E had the lowest serum TG and TC concentrations, while that of rats in groups ET and T showed a increasing trend. The serum concentrations of LDL-C significantly increased in rats of groups ET and T, but the serum HDL-C concentraions of rats in group E increased.(3) Results of HE and Oil Red0staining of the liver tissues demonstrated that2,3,7,8-TCDD may induce obvious pathological changes and lipid infiltration, which can be improved by long-term exercise.(4) Continuous exposure to2,3,7,8-TCDD may significantly increase mRNA and protein expression of enzymes (ACC1, FAS, SCD1) and transcription factors (LXRa, SREBP-1) related to the lipid synthesis. Long-term exercise may significantly decrease the above mRNA and protein expressions. The interactions in some indicators between2,3,7,8-TCDD exposure and exercise treatment showed an antagonistic effect.
Conclusions:(1)2,3,7,8-TCDD may effect on the mRNA and protein expression of the hepatic lipid anabolic related enzymes and transcription factors, and consequently result in the lipid metabolism disorders.(2) The influences of exercise on hepatic lipid anabolic showed a time effect, indicated that only long-term exercise might have an evident effect. However, further studies should be conducted to investigate the mechanism.(3) Long-term exercise can improve the lipid metabolic disorders caused by2,3,7,8-TCDD.
结果：1、各组大鼠精神状态良好,活动能力正常；体重呈增长趋势,但每组趋势不同；ET、T组肝脏相对重量显著增高；4w末ET组附睾脂肪相对重量显著下降。2、2,3,7,8-TCDD可显著增高肝脏TG含量,长期运动可显著降低肝脏TG含量；各组大鼠中E组血清TG、TC含量最低,ET、T组血清TG、TC呈增长趋势；ET、T组血清LDL-C显著增高,长期运动E组血清HDL-C显著增高。3、肝脏组织HE和油红0染色显示2,3,7,8-TCDD可诱发明显病理性改变及脂质浸润,长期运动可改善病理性改变及脂质浸润情况。4、2,3,7,8-TCDD持续染毒可显著增加脂质合成代谢过程中相关酶ACC1、FAS、SCD1及转录因子LXRa、SREBP-1的mRNA和蛋白表达,长期运动可显著降低染毒大鼠ACC1、FAS、SCD1、LXRa、SREBP-1的mRNA和蛋白表达,2,3,7,8-TCDD染毒和运动对某些指标的影响存在交互作用,表明运动有干预效应。
结论：1、2,3,7,8-TCDD影响肝脏脂质合成代谢过程中相关酶及转录因子的mRNA和蛋白表达,从而造成脂质代谢紊乱。2、运动对肝脏脂质合成代谢的影响具有时间效应,长期运动才明显体现,其具体影响机制还需进一步研究。3、长期运动可改善2,3,7,8-TCDD造成脂质代谢的紊乱。
Objective:This paper investigated the effects of exercise and2,3,7,8-TCDD on the hepatic lipid anabolic in the SD rats, and explored the mechanism of the effects. The aim is to develop a new knowledge of the pathogenesis of metabolic diseases, and provide a corresponding support for the hypothesis that exercise might prevent the metabolic deseases caused by dioxin-like environmental pollutants.
Methods:SD rats (8weeks old) were divided into4different groups randomly:control group (C), exercise group (E), T group treated with2,3,7,8-TCDD, and ET group treated with exercise and2,3,7,8-TCDD. Rats in groups T and ET were intraperitoneally given2,3,7,8-TCDD in a first dose of6.4μg/kg·bw, and thereafter, they were administrated a mantenance dosage by21%of the first dose weekly for7weeks. Groups E and ET swam30min every day with a tail load of5%body weight for5days per week. The healthy status of the rats was daily observed. The blood samples were collected through the inner canthus vein at the end of1st,3rd,4th,6th,8th weekends, and then the serum lipid indicators were measured. Half groups of the rats were killed at the4th and8th weekends respectively, and the relative weights of the organs were calculated. The liver tissues were collected to conduct the biochemical parameter measurements and histopathological detections. ACC1, FAS, SCD1, LXRa, SREBP-1, ChREBP mRNA and protein expression were determined via real-time quantitative PCR and Western Blot methods.
Results:(1) All rats were in good mental state and normal activity. And the body weights showed a growth trend, but there were differences between each group. The relative weights of the livers significantly increased in the rats of groups ET and T. The relative weights of epididymal fat greatly decreased at the4th weekend in the rats of group ET.(2)2,3,7,8-TCDD showed a significantly increasing effect on the TG concentrations in livers, while long-term exercise might have a deceasing effect. Rats in group E had the lowest serum TG and TC concentrations, while that of rats in groups ET and T showed a increasing trend. The serum concentrations of LDL-C significantly increased in rats of groups ET and T, but the serum HDL-C concentraions of rats in group E increased.(3) Results of HE and Oil Red0staining of the liver tissues demonstrated that2,3,7,8-TCDD may induce obvious pathological changes and lipid infiltration, which can be improved by long-term exercise.(4) Continuous exposure to2,3,7,8-TCDD may significantly increase mRNA and protein expression of enzymes (ACC1, FAS, SCD1) and transcription factors (LXRa, SREBP-1) related to the lipid synthesis. Long-term exercise may significantly decrease the above mRNA and protein expressions. The interactions in some indicators between2,3,7,8-TCDD exposure and exercise treatment showed an antagonistic effect.
Conclusions:(1)2,3,7,8-TCDD may effect on the mRNA and protein expression of the hepatic lipid anabolic related enzymes and transcription factors, and consequently result in the lipid metabolism disorders.(2) The influences of exercise on hepatic lipid anabolic showed a time effect, indicated that only long-term exercise might have an evident effect. However, further studies should be conducted to investigate the mechanism.(3) Long-term exercise can improve the lipid metabolic disorders caused by2,3,7,8-TCDD.
引文
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