玉米功能肽的制备及其生理活性的研究
摘要
分别以碳酸氢钠脱脂后的玉米蛋白粉和乙醇浸提的玉米醇溶蛋白为原料,以一种工业用碱性蛋白酶为催化剂,制备了主要分子量为400-600道尔顿、富含谷氨酸、丙氨酸和亮氨酸的CPS和ZPS两种玉米肽产品。利用Sephedax G-15、G-10葡聚糖凝胶层析法和HPLC法对CPS和ZPS进行了分离纯化,得到PCPS、PZPS、HPCPS和HPZPS四种主要组分。检测了其中抗氧化能力最高的活性组分—PCPS和PZPS的分子量分布情况和氨基酸组成情况。
以α-生育酚为阳性对照,研究了CPS、ZPS、PCPS、PZPS、HPCPS和HPZPS对DPPH自由基、超氧阴离子自由基、羟自由基的清除作用及对亚油酸氧化的抑制作用,证明玉米肽具有抗氧化性。通过对以上各组分还原力的测定进一步证明了玉米肽的还原剂性质。
采用Vallee&Hoch法,通过离体实验和动物实验,研究了玉米肽对乙醇代谢的两种酶—AND和ALDH的影响。通过建立酒精氧化性肝损伤模型,证明了玉米肽对氧化性肝损伤的辅助保护作用,初步探讨了玉米肽迅速调节乙醇代谢的机理为:提高了AND和ALDH的活力及结合了乙醇代谢的中间产物—乙醛。同时研究了玉米肽抗疲劳功效和对乳腺癌细胞增殖的影响。
在以上实验结果的基础上,对玉米功能肽的制备方法进行了放大实验研究,采用微滤和超滤技术对玉米肽粗制品进行了精制处理,利用正交设计方法研制了玉米肽饮料配方。通过功能性检验、卫生学检验、毒理学实验和理化性质分析,证明了玉米肽产品的功效性、功效成分的稳定性和食用安全性,为其规模化生产提供了理论依据。
Free radicals are the dissociative molecules, atoms or ions with unpaired electron,having an active chemical property. They always attact on protein and lipid, subsequently form new other free radicals and lipid peroxide, which damage the organism. Under the normal physiological condition, free radicals are constantly produced and constantly scavenged by the antioxidants in the organism, occupying the state of dynamic equilibrium. Once the equilibrium is broken by the augment of oxygen free radicals, or the descent of antioxidant level and activity, the organism is then injuried. Therefore, it is necessary to supply the exogenous oxygen free radical scaverger to keep the equilibrium. Recently, more and more researchers pay close attention to natural exogenous antioxidants.
In the present study, CPS (corn peptides) and ZPS (zein peptides) were prepared from defatted corn gluten meal and zein respectively by proteolysis with an alkaline protease. The activest members of CPS and ZPS, PCPS and PZPS were separated by Sephadex G15, G10 and HPLC column method. Their antioxidant abilities were confirmed by their scavenging effects of the 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical, superoxide anion radical, hydroxyl radical and inhibition of linoleic acid autooxidation. Their reducing powers were also analyzed. The EC50 of PCPS and PZPS of scavenging DPPH were 1.05mg/ml and 1.04mg/ml. The EC50 of scavenging superoxide anion radical of them were 10.94mg/ml and 10.85mg/ml. The EC50 of scavenging hydroxyl radical of them were 0.73mg/ml and 0.73mg/ml. The results indicated that PCPS and PZPS possessed the similar antioxidant abilities.
The molecular-mass distributions of CPS, ZPS, PCPS and PZPS were determined and compared by MS. The main molecular-mass distributions of them were from 400 to 600 Da. Their amino acid compositions were also analyzed by HPLC. Glutamic acid, leucine and alanine were rich in CPS, ZPS, PCPS and PZPS.
8.38% fat in the corn gluten meal delayed the process of super-filtration and increased the cost during preparation of CPS. Sodium carbonate and sodium bicarbonate were chosen as the defatting reagent to avoid the high cost and hazard of organic solvent and supercritical CO2 extraction technology. The best defatting technology of sodium bicarbonate by orthogonal test was determined: 1g sifted(250μm)corn gluten meal powder was mixed with 12ml 80g/l sodium bicarbonate for 20min at 40℃, the defatting rate was 81.73%.
At present, alcohol abuse and alcoholism are serious health and socioeconomic problems throughout the world. Drunkenness is a symptom that transfer function of central nerve system is broken induced by the increase of the concentration of ethanol in the plasma. Therefore, study on sobering medicament is very important.
It is well known that the principal organ responsible for the elimination of ingested alcohol is the liver. Alcohol is metabolized by alcohol dehydrogenase (ADH) enzyme system-catalyzed oxidative processes. ADH, as the first enzyme in alcohol metabolism process, its levels and activities has very important effect on the metabolism of alcohol in the organism. Therefore, the accelerating effects of CPS, ZPS, PCPS and PZPS to ADH activities in vitro were determined in the present study when using glutathione (GSH) as the positive control by Vallee & Hoch. The results indicated that the accelerating effect of PCPS to ADH activities was super to GSH. The results also indicated that PCPS possessed good ability of increasing ADH activities in the presence of pyrazole, the inhibitor of ADH. The results of in vivo experiments also indicated that CPS possessed good ability of increasing ADH activities
The results of influence of CPS to aldehyde dehydrogenase (ALDH) activities in vitro did not proved CPS accelerating ALDH activities since PCPS reacted to aldehyde. But the results of in vivo experiments indicated that CPS possessed good ability of increasing ALDH activities. The data showed that PCPS accelerated activities of ALDH.
Using Haiwangjinzun, a sobering medicament, as positive control, the protective effect of CPS against acute hepatic injuries induced by alcohol was verified in NH mice that were fed with different dosage of CPS for 30 days and given an acute dose of alcohol orally subsequently.
As a result, CPS reduced both hepatic malondialdehyde and triacylglycerol levels along with enhanced hepatic GSH level relative to the control. Hepatic histological changes were also observed. The result indicates that CPS is capable of attenuating ethanol-induced hepatic injury. The possible cause was that CPS enhanced ADH and ALDH activities and consequently accelerared alcohol metabolism, subsequently protected liver.
Many researchers reported anti-fatigue of CPS. therefore, Using ginsenoside as positive control, effect of alleviate fatigue of CPS was also determined in the present study. The data showed that CPS extended swimming time, increased level of liver glycogen, reduced levels of plasma urea and plasma lactates of NH mice significantly.
Yamaguch et al reported that CPS can inhibit process of breast carcinoma, the influence of CPS to proliferation of breast human carcinoma cell line MCF-7 was also analyzed in the present study. The result indicated that CPS did not inhibit proliferation of breast human carcinoma cell line MCF-7. It was possible that CPS scavenged the free radicals, which participate in the course of cancerous treatment. Consequently, process of breast carcinoma was inhibited.
Finally, the above preparation of active CPS was scaled up. The physical and chemical indicators of corn peptides were determined. The stability of functional component, peptide, was also proved, including antioxidant properties. The histological observation on toxicity of CPS indicated that CPS did not do harm to heart, liver, spleen, lung, kidney, stomach, duodenum, testicle and ovary. The formula of corn peptides beverage determined by orthogonal test was: 20g/l corn peptides,80g/l xylitol and 2g/l malic acid.
All in all, the results obtained in the present study can lay academic and practical foundation for its application to pharmaceutical and food systems as a functional additive in the future.
以α-生育酚为阳性对照,研究了CPS、ZPS、PCPS、PZPS、HPCPS和HPZPS对DPPH自由基、超氧阴离子自由基、羟自由基的清除作用及对亚油酸氧化的抑制作用,证明玉米肽具有抗氧化性。通过对以上各组分还原力的测定进一步证明了玉米肽的还原剂性质。
采用Vallee&Hoch法,通过离体实验和动物实验,研究了玉米肽对乙醇代谢的两种酶—AND和ALDH的影响。通过建立酒精氧化性肝损伤模型,证明了玉米肽对氧化性肝损伤的辅助保护作用,初步探讨了玉米肽迅速调节乙醇代谢的机理为:提高了AND和ALDH的活力及结合了乙醇代谢的中间产物—乙醛。同时研究了玉米肽抗疲劳功效和对乳腺癌细胞增殖的影响。
在以上实验结果的基础上,对玉米功能肽的制备方法进行了放大实验研究,采用微滤和超滤技术对玉米肽粗制品进行了精制处理,利用正交设计方法研制了玉米肽饮料配方。通过功能性检验、卫生学检验、毒理学实验和理化性质分析,证明了玉米肽产品的功效性、功效成分的稳定性和食用安全性,为其规模化生产提供了理论依据。
Free radicals are the dissociative molecules, atoms or ions with unpaired electron,having an active chemical property. They always attact on protein and lipid, subsequently form new other free radicals and lipid peroxide, which damage the organism. Under the normal physiological condition, free radicals are constantly produced and constantly scavenged by the antioxidants in the organism, occupying the state of dynamic equilibrium. Once the equilibrium is broken by the augment of oxygen free radicals, or the descent of antioxidant level and activity, the organism is then injuried. Therefore, it is necessary to supply the exogenous oxygen free radical scaverger to keep the equilibrium. Recently, more and more researchers pay close attention to natural exogenous antioxidants.
In the present study, CPS (corn peptides) and ZPS (zein peptides) were prepared from defatted corn gluten meal and zein respectively by proteolysis with an alkaline protease. The activest members of CPS and ZPS, PCPS and PZPS were separated by Sephadex G15, G10 and HPLC column method. Their antioxidant abilities were confirmed by their scavenging effects of the 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical, superoxide anion radical, hydroxyl radical and inhibition of linoleic acid autooxidation. Their reducing powers were also analyzed. The EC50 of PCPS and PZPS of scavenging DPPH were 1.05mg/ml and 1.04mg/ml. The EC50 of scavenging superoxide anion radical of them were 10.94mg/ml and 10.85mg/ml. The EC50 of scavenging hydroxyl radical of them were 0.73mg/ml and 0.73mg/ml. The results indicated that PCPS and PZPS possessed the similar antioxidant abilities.
The molecular-mass distributions of CPS, ZPS, PCPS and PZPS were determined and compared by MS. The main molecular-mass distributions of them were from 400 to 600 Da. Their amino acid compositions were also analyzed by HPLC. Glutamic acid, leucine and alanine were rich in CPS, ZPS, PCPS and PZPS.
8.38% fat in the corn gluten meal delayed the process of super-filtration and increased the cost during preparation of CPS. Sodium carbonate and sodium bicarbonate were chosen as the defatting reagent to avoid the high cost and hazard of organic solvent and supercritical CO2 extraction technology. The best defatting technology of sodium bicarbonate by orthogonal test was determined: 1g sifted(250μm)corn gluten meal powder was mixed with 12ml 80g/l sodium bicarbonate for 20min at 40℃, the defatting rate was 81.73%.
At present, alcohol abuse and alcoholism are serious health and socioeconomic problems throughout the world. Drunkenness is a symptom that transfer function of central nerve system is broken induced by the increase of the concentration of ethanol in the plasma. Therefore, study on sobering medicament is very important.
It is well known that the principal organ responsible for the elimination of ingested alcohol is the liver. Alcohol is metabolized by alcohol dehydrogenase (ADH) enzyme system-catalyzed oxidative processes. ADH, as the first enzyme in alcohol metabolism process, its levels and activities has very important effect on the metabolism of alcohol in the organism. Therefore, the accelerating effects of CPS, ZPS, PCPS and PZPS to ADH activities in vitro were determined in the present study when using glutathione (GSH) as the positive control by Vallee & Hoch. The results indicated that the accelerating effect of PCPS to ADH activities was super to GSH. The results also indicated that PCPS possessed good ability of increasing ADH activities in the presence of pyrazole, the inhibitor of ADH. The results of in vivo experiments also indicated that CPS possessed good ability of increasing ADH activities
The results of influence of CPS to aldehyde dehydrogenase (ALDH) activities in vitro did not proved CPS accelerating ALDH activities since PCPS reacted to aldehyde. But the results of in vivo experiments indicated that CPS possessed good ability of increasing ALDH activities. The data showed that PCPS accelerated activities of ALDH.
Using Haiwangjinzun, a sobering medicament, as positive control, the protective effect of CPS against acute hepatic injuries induced by alcohol was verified in NH mice that were fed with different dosage of CPS for 30 days and given an acute dose of alcohol orally subsequently.
As a result, CPS reduced both hepatic malondialdehyde and triacylglycerol levels along with enhanced hepatic GSH level relative to the control. Hepatic histological changes were also observed. The result indicates that CPS is capable of attenuating ethanol-induced hepatic injury. The possible cause was that CPS enhanced ADH and ALDH activities and consequently accelerared alcohol metabolism, subsequently protected liver.
Many researchers reported anti-fatigue of CPS. therefore, Using ginsenoside as positive control, effect of alleviate fatigue of CPS was also determined in the present study. The data showed that CPS extended swimming time, increased level of liver glycogen, reduced levels of plasma urea and plasma lactates of NH mice significantly.
Yamaguch et al reported that CPS can inhibit process of breast carcinoma, the influence of CPS to proliferation of breast human carcinoma cell line MCF-7 was also analyzed in the present study. The result indicated that CPS did not inhibit proliferation of breast human carcinoma cell line MCF-7. It was possible that CPS scavenged the free radicals, which participate in the course of cancerous treatment. Consequently, process of breast carcinoma was inhibited.
Finally, the above preparation of active CPS was scaled up. The physical and chemical indicators of corn peptides were determined. The stability of functional component, peptide, was also proved, including antioxidant properties. The histological observation on toxicity of CPS indicated that CPS did not do harm to heart, liver, spleen, lung, kidney, stomach, duodenum, testicle and ovary. The formula of corn peptides beverage determined by orthogonal test was: 20g/l corn peptides,80g/l xylitol and 2g/l malic acid.
All in all, the results obtained in the present study can lay academic and practical foundation for its application to pharmaceutical and food systems as a functional additive in the future.
引文
[1] Harman D. Aging: A theory based on free radical and radiation chemistry [J]. Journal of Gerontology, 1956, 11 (3):298-300.
[2] Ji LL. Antioxidants and oxidative stress in exercise [J]. Proceedings of the Society for Experimental Biology and Medicine, 1999, 222 (3):283-292.
[3] 郑荣梁.自由基生物学[M].高等教育出版社:1992,第1版,21.
[4] Shinohara R, Mano T, Nagasaka A, Hayashi R, Uchimura K, Nakano I, Watanabe F, Tsugawa T, Makino M, Kakizawa H, Nagata M, Iwase K, Ishizuki Y and Itoh M. Lipid peroxidation levels in rat cardiac muscle are affected by age and thyroid status [J]. Journal of Endocrinology, 2000, 164 (1):97-102.
[5] 赵克然 ,杨毅军 ,曹道俊 .氧自由基与临床 [M].中国医药科技出版社:2000,第1版,9-10.
[6] Svingen BA, Neal FO and Aust SD. The role of superoxide and singlet oxygen in lipid peroxidation [J]. Photochemistry and Photobiology, 1978, 28 (4-5):803-809.
[7] Boveris A, Cadenas E. Production of superoxide radicals and hydrogen peroxide in mitochondria [M]. Oberley LW ed, Superoxide Dismutase. Florida: CRC Press, 1982, 2:15-30.
[8] Fridovich I. Measuring the activity of superoxide dismutase: An emvarrassment of riches [M]. In: Oberley LW ed, Superoxide Dismutase. Florida: CRC Press, 1982, 2:69-78.
[9] Bors W. In current topics in radiation research quarterly [M]. Armsterdam: North-Holland, Florida, 1974, 9:247.
[10] Ohnishi T, Blum H, Salerno JC. In function of quinones in energy-conserving systems [M]. Trumpower BL ed, New York: Academic Press, 1980.
[11] Halliwell B and Gutteridge JMC. Free radicals in biology andmedicine [M]. Oxford Clarendon Press: 1989, 110.
[12] Bors W, Saran M and Czapski G. The nature of intermediates during biological oxygen activation [M]. In: Bannister WH, Bannister JW ed, Biological and clinical aspects of superoxide and superoxide dismutase, 1980, 1-31.
[13] Cohen G and Cederbrum AI. Chemical evidence for production of hydroxyl radicals during microsomal electron transfer [J]. Science, 1979, 204:66-68.
[14] Bors W, Saran M, Michel C. Assay of oxygen radical method and mechanisms [M]. Obeley LW ed, Superoxide Dismutase Floreda: CRC Press, 1989, 31-62.
[15] Ekstr?m G, Cronholm T and Ingelman-Sundberg M. Hydroxyl-radical production and ethanol oxidation by liver microsomes isolated from ethanol-treated rat [J]. Biochemical Journal, 1986, 233:755-761.
[16] Fridovich I. Free radicals in biology [M]. Pryor WA ed, New York: Academic Press, 1976, 1:239.
[17] Kanofsky JR. Singlet oxygen production in superoxide ion-halocarbon systems [J]. Journal of the American Chemical Society, 1986, 108(11):2977-2979.
[18] 赵 保 路 . 氧 自 由 基 和 天 然 抗 氧 化 剂 [M]. 科 学 出 版 社 :2002, 第 1版,77-83.
[19] 赵燕 ,江文 ,侯京武 ,赵保路 ,文娟 .钙过负荷和丹参酮对线粒体脂质 过 氧 化 的 影 响 [J]. 生 物 化 学 与 生 物 物 理 学 报 ( 英 文版),1995,27(6):610-615.
[20] 赵 保 路 . 肿 瘤 细 胞 膜 的 流 动 性 [J]. 国 外 医 学 分 子 生 物 学 分册,1984,6(5):215-218.
[21] Nikola Getoff. Factors influencing the efficiency of radiationinduced degradation of water pollutants [J]. RadiationPhysics and Chemistry, 2002, 65(4-5):437-446.
[22] 郑 建 仙 .功 能 性 食 品 ( 1卷 ) [M].中 国 轻 工 业 出 版 社 :1999,第 1版,234-247.
[23] Pryor W A. Free radical biology: xenobiotics, cancer and aging [J]. Annals of the New York Academy of Sciences, 1982, 393(1):1-22.
[24] Lomnitski L, Bergman M, Schon I and Grossman S. The effect of dietary vitamin E and β-carotene on oxidantion processes in the rat testis [J]. Biochemical et Biophysical Acta, 1991, 1082(1):101-107.
[25] 崔剑,李兆龙,洪啸吟.自由基生物抗氧化与疾病[J].清华大学学报(自然科学版),2000,40(6):9-12.
[26] Hass HM and Massaro D. Differences in Cu, Zn superoxide dismutase induction in lungs of neonatal and adult rats [J]. American Journal of Physiology Cell Physiology, 1987, 253:66-70.
[27] Natvig DO, Imlay K, Touati D and Hallewell RA. Human copper-zinc superoxide dismutase complements superoxide dismutase-deficient Escherichia coli mutants [J]. Journal of Biological Chemistry, 1987, 262(30):14697-14701.
[28] Kirkman HN, Galiano S, Gaetani GF. The function of catalase-bound NADPH [J]. Journal of Biological Chemistry, 1987, 262(2):660-666.
[29] Anonymous A. A termination codon specifies selenocysteine in glutathione peroxidase [J]. Nutrition Reviews, 1988, 46:202-230.
[30] Anisimov VN, Zavarzain NY. Melatonin increases both life span and tumor incidence in female CBA mice [J]. Journal of Gerontology Series A-Biological Sciences and Medical Sciences, 2001, 56(7):311-323.
[31] Kopánia M, Celec P, Danixovi L, Michalka P and Biró C. Oxidative stress and electron spin resonance [J]. Clinica ChimicaActa, 2006, 364:61–66.
[32] Kong J-M, Li L-Z, Xie K-S and Wu W-K. Antioxidant capacity measurement of flavonoids by chemil uminescence method [J]. Natural Product Research and Development, 2004, 16(1):46-49.
[33] Laurent D, Marie-Héléne L and Avishay-Abraham S. High-performance liquid chromatographic assay of hydroxyl free radical using salicylic acid hydroxylation during in vitro experiments involving thiols [J]. Journal of Chromatography B, 2001, 763:185-193.
[34] 郑雄敏,徐旭士.茶多酚对豆油及菜油抗氧化性的研究[J].南昌大学学报(理科版),1998,22(2):105-108.
[35] 韩丽华,王丽红,范希王月.大豆异黄酮抗氧化性的研究 [J].中国油脂,2001,26(6):41-44.
[36] 魏安池,周瑞宝,瞿水忠.诃子抗氧化性能的研究[J].郑州粮食学院学报,1998,19(1):8-12.
[37] 王文中,王颖.迷迭香的研究及其应用—抗氧化剂[J].中国食品添加剂,2002,(5):60-65.
[38] 金鸣,蔡亚欣,李金荣,赵辉.邻二氮菲-Fe2+氧化法检测H2O2/Fe2+产 生 的 羟 自 由 基 [J]. 生 物 化 学 与 生 物 物 理 进展,1996,23(6):553-555.
[39] 贾之慎,邬建敏,唐孟成.比色法测定Fenton反应产生的羟自由基[J].生物化学与生物物理进展,1996,23(2):184-186.
[40] 于守洋,崔鸿斌,石华.中国营养保健食品指南[M].黑龙江科学技术出版社:1996,第1版,50-51.
[41] Delgado-Zamarre?o MM, González-Maza I, Sánchez-Pérez A and Carabias Martínez R. Analysis of synthetic phenolic antioxidants in edible oils by micellar electrokinetic capillary chromatography [J]. Food Chemistry, 2007, 100:1722–1727.
[42] Jun S-Y, Park P-J, Jung W-K and Kim S-K. Purification andcharacterization of an antioxidative peptide from enzymatic hydrolysate of yellowfin sole (Limanda aspera) frame protein [J]. European Food Research and Technology, 2004, 219:20-26.
[43] 佘世望,肖小年,范青生,吴凌伟,贺新兴,詹志军.60种药食两用植物抗氧化作用研究[J].食品科学,1995,16(11): 3-5.
[44] Uthrie N, Kurowska EMN. Anticancer and cholesterol-lowering activities of citrus flavonoids [M].Handbook of Nutraceuticals and Functinoal Foods. CRC Press: Boca Raton, Florida, 2001, 113-126.
[45] Du Toit R, Volsteedt Y and Apostolides Z. Comparison of the antioxidant content of fruits, vegetables and teas measured as vitamin C equivalents [J]. Toxicology, 2001, 166(1-2):63-69.
[46] Kim S-K, Kim Y-T, Byun H-G, Nam K-S, Joo D-S and Shahidi F. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin [J]. Journal of Agricultural and Food Chemistry, 2001, 49(4):1984–1989.
[47] Thiansilakul Y, Benjakul S and Shahidi F, Compositions, functional properties and antioxidative activity of protein hydrolysates prepared from round scad (Decapterus maruadsi)[J]. Food chemistry, 2007, 103:1385-1394.
[48] Klompong V, Benjakul S, Kantachote D and Shahidi F. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type [J]. Food Chemistry, 2007, 102:1317-1327.
[49] Velloglu YS, Mazza G, Gao L and Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products [J]. Journal of Agricultural and Food Chemistry, 1998, 46: 4113-4117.
[50] 丁利君,周燕芳,林楠.溪黄草中黄酮类物质提取及对羟自由基清除作用[J].食品与机械,2004,20(3):13-14.
[51] 蔡为荣.微波提取荷叶黄酮及其清除羟自由基的研究[J].食品科学,2004,25(9):112-115.
[52] 赵瑞艳,徐雅琴.树莓叶片中黄酮类物质提取及抗氧化性研究[J].食品科学,2006,22(2):104-106.
[53] 杨锦华,李博,籍保平.我国常见食用和药用植物的抗氧化性研究[J].食品科学,2006,27(6):87-91.
[54] Wang J-S, Zhao M-M, Zhao Q-Z and Jiang Y-M. Antioxidant properties of papain hydrolysates of wheat gluten in different oxidation systems [J]. Food Chemistry, 2007, 101:1658-1663.
[55] Zhu K, Zhou H. and Qian H. Antioxidant and free raical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase [J]. Process Biochemistry, 2006, 41:1296-1302.
[56] Gibbs BF, Zougman A, Masse R and Mulligan C. Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food [J]. Food Research International, 2004, 37:123-131.
[57] Rüfer CE and Kulling SE. Antioxidant activity of isoflavones and their major metabolites using different in vitro assays [J]. Journal of Agricultural and Food Chemistry, 2006, 54:2926-2931.
[58] 韩立强,朱松波,杨国宇.肌肽—一种有待开发的天然抗氧化剂[J].中国畜牧杂志,2005,41(2):33-35.
[59] 聂少平,谢明勇,罗珍.茶叶多糖的抗氧化活性研究[J].天然产物研究与开发,2005,17(5):549-552.
[60] 胡庭俊 ,程富胜 ,陈炅然 ,梁纪兰 ,董鹏程 .山豆根多糖体外清除自由基作用的研究[J].中兽医医药杂志,2004,(5):6-8.
[61] 王辉,罗顺德,刘刚,陈伟.莲心碱对氧自由基和脂质过氧化的影响[J].中国药理学通报,2004,20(10):1197-1198.
[62] 李丹 ,肖刚 ,丁霄霖 .苦荞黄酮清除自由基作用的研究 [J].食品科技,2000,(6):61-64.
[63] 张 燕 平 . 天 然 蜂 胶 对 自 由 基 的 清 除 作 用 [J]. 食 品 与 发 酵 工业,2002,28(1):48-52.
[64] 李蜀眉 ,宋刚 ,田维平 ,塔娜 ,傅忠实 .桔皮中黄酮化合物清除自由基的研究[J].内蒙古农业大学学报,2004,25(3):89-91.
[65] 武文洁,吕树祥,宋诗莹.芝麻提取物抗氧化性能研究[J].中国食品添加剂,2004,(1):70-72,66.
[66] 仇洁,周永标,谭玉兰,叶红梅.落葵薯提取物清除氧自由基及抗脂质过氧化作用[J].中药材,2004,27(8):608-609.
[67] 钟海雁 ,李忠海 ,魏元青 .林檎叶提取物抗氧化及·OH自由基清除作用的研究[J].北华大学学报(自然科学版),2001,2(6):522-525.
[68] 杨广花.两种天然抗氧化剂清除自由基作用的研究[J].江苏工业学院学报,2004,16(3):28-30.
[69] Venant Nihorimbere,钱和,汪何雅,陈军杰,梁清蓉.DPPH自由基比色 法 测 定 番 石 榴 叶 提 取 物 抗 氧 化 活 性 的 研 究 [J]. 食 品 科学,2004,25(7):36-42.
[70] 田云 ,卢向阳 ,易克 ,何小解 ,方俊 .天然抗氧化剂清除氧自由基的能力[J].湖南农业大学学报(自然科学版),2004,30(3):209-211.
[71] 肖丽霞,张建良.槐花抗氧化剂的提取及其抗氧化性的测定[J].江苏农学院学报,1996,17(2):71-73.
[72] 郭亚力 ,李聪 ,欧灵澄 ,王文久 ,黎勇坤 .槐米中天然抗氧化剂的提取及其抗自由基性能研究[J].食品科学,2004,25(7):154-157.
[73] 冯兰萍 ,刘在群 ,周波 ,杨立 ,刘中立 .绿茶多酚对自由基诱导的红细 胞 氧 化 性 溶 血 的 抑 制 作 用 [J]. 科 学 通报,2000,45(12):1271-1275.
[74] 张健 ,秦静芬 ,曹恩华 ,张仲伦 ,郑雁珍 .DNA损伤的化学发光法测定 和 茶 多 酚 对 它 的 保 护 作 用 [J]. 生 物 物 理 学报,1996,12(4):691-695.
[75] 方若莹,俞越汉,沈公羽.茶多酚对沙土鼠脑缺血后再灌注氧化损伤的保护作用[J].生物物理学报,1997,13(2):309-314.
[76] 叶汉侠,王甫才.18种中草药抗氧化活性的比较研究[J].浙江万里学院学报,2004,17(5):111-113.
[77] 刘玉鹏 ,刘梅 ,刘俊英 ,翁新楚 .30种中草药的抗氧化活性研究 [J].烟台大学学报(自然科学与工程版),2000,13(1):70-73.
[78] Cai Yizhong, Luo Qiong, Sun Mei and Corke Harold. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer [J]. Life Sciences, 2004, 74(17):2157-2184.
[79] 何文珊,李炎,李琳,郭祀远.几种香辛料对油脂抗氧化效果的评价[J].中国调味品,1998, (7): 12-15.
[80] Tsai Tzung-Hsun, Tsai Po-Jung and Ho Su-Chen. Antioxidant and anti-inflammatory activities of several commonly used spices [J]. Journal of Food Science, 2005, 70:93-97.
[81] Prasad K, Laxdal VA, Yu M and Raney BL. Evaluation of hydroxyl radical-scavenging property of garlic [J].Molecular and Cellar Biochemistry, 1996, 154(1):55-63.
[82] 黄水清,徐志伟.当归补血汤及当归黄芪抗氧化和抑制自由基产生的研究进展[J].中华实用中西医杂志,2004,4(17)(9):1274-1276.
[83] 蔡溱,胡晋红,全山丛,陈士明.芪灵胶囊清除超氧阴离子自由基的作用[J].中国医院药学杂志,2004,24(7):401-402.
[84] 孙冀平,沈蓓英.蛋白肽饮料的醒酒机理和制备工艺的探讨[J].食品与发酵工业,1999,25(4):64-66.
[85] Lieber CS and Decarli LM. Hepatic microsomal ethanol-oxidizing system In vitro characteristics and adaptive properties in vivo [J]. Journal of Biological Chemistry, 1970, 245(10):2505-2512.
[86] Handler JA and Thurman RG. Catalase-dependent ethanol oxidation in perfused rat liver. Requirement forfatty-acid-stimulated H202 production by peroxisomes [J]. European Journal of Biochemistry, 1988, 176:477-484.
[87] 刘 根 生 . 不 同 人 种 酒 精 代 谢 机 理 的 差 异 [J]. 生 物 学 教学,1997,(11):43,2.
[88] 蒋业贵,李兆申.酒精性肝病发病机制的研究进展[J].胃肠病学和肝病学杂志,2003,12(5):484-486.
[89] Babu PP, Kumari LR and Vemuri MC. Differential changes in cell morphology, macromolecular composition and membrane protein profiles of neurons and astrocytes in chronic ethanol treated rats [J]. Molecular and Cellular Biochemistry, 1994, 130(1):29-40.
[90] 厉 有 名 . 酒 精 性 肝 病 的 发 病 机 制 [J]. 中 华 肝 脏 病 杂志,2003,11(11):690-691.
[91] Valenzuela A, Lagos C, Schmidt K and Videla LA. Silymarin protection against hepatic lipid peroxidation induced by acute ethanol intoxication in the rat [J]. Biochemical Pharmacology, 1985, 34(12):2209-2212.
[92] 赵善民 ,黄丽娟 ,黄俊杰 ,何显教 ,晋玲 .乙醇对兔心脏活动与氧自由基的影响[J].心脏杂志,2003,15(1):7-8,11.
[93] 高小玲,尹少甫,张本忠.乙醇、乙醛对红细胞膜ATP酶活性影响的体外实验研究[J].中国工业医学杂志,2004,17(4):233-235.
[94] Situnayake RD, Crump BJ, Thurmham DI, Davies JA, Gearty J and Davis M. Lipid peroxidation and hepatic antioxidants in alcoholic liver disease [J]. Gut, 1990, 31:1311-1317.
[95] H??g J-O, Hedberg JJ, Str?mberg P and Svensson S. Mammalian alcohol dehydrogenase-functional and structural implications [J]. Journal of Biomedical Science, 2001, 8(1):71-76.
[96] Tuma DJ and Sorrell M. Alcohol and Alcoholic liver disease [J]. Seminars in Liver Disease, 2004, 24(03):215-216.
[97] Tuma DJ and Casey CA. Dangerous byproducts of alcoholbreakdown-focus on adducts [J]. Alcohol Research & Health, 2003, 27(4):285-290.
[98] Duarte R, Escario JJ. Alcohol abuse and truancy among Spanish adolescents: Acount-data approach [J]. Economics of Education Review, 2006, 25(2):179-187.
[99] Chatterji P, Dave D, Kaestner R, Markowitz S. Alcohol abuse and suicide attempts among youth [J]. Economics & Human Biology, 2004, 2(2):159-180.
[100] Rehm J, Room R, Brink WV, Jacobi F. Alcohol use disorders in EU countries and Norway: An overview of the epidemiology [J]. European Neuropsychopharmacology. 2005, 15(4):377-388.
[101] Zhang Xiaochun, Li Shi-yan, Brown RA, Ren Jun. Ethanol and acetaldehyde in alcoholic cardiomyopathy: from bad to ugly en route to oxidative stress [J]. Alcohol, 2004, 32(3):175-186.
[102] Pettinatia HM and Rabinowitz AR. Recent advances in the treatment of alcoholism [J]. Clinical Neuroscience Research, 2005, 5:151-159.
[103] 张若明 ,李经才 .解酒天然药物研究进展 [J].沈阳药科大学学报,2001,18(2):138-142.
[104] Lipsky JJ, Shen ML and Naylor S. In vivo inhibition of aldehyde dehydrogenase by disulfiram [J]. Chemico-Biological Interactions, 2001, 130-132:93-102.
[105] Thomas CP, Wallack SS, Lee S, McCarty D and Swift R. Research to practice: adoption of naltrexone in alcoholism treatment [J]. Journal of Substance Abuse Treatment. 2003, 24(1):1–11.
[106] Davidson D, Saha C, Scifres S, Fyffeb J, Connora S and Selzer C. Naltrexone and brief counseling to reduce heavy drinking in hazardous drinkers [J]. Addictive Behaviors, 2004, 29:1253–1258.
[107] Boothby LA, Pharm D, Doering PL. Acamprosate for the Treatment of Alcohol Dependence [J]. Clinical Therapeutics, 2005, 27(6):695-714.
[108] Yokozawa T, Dong E, Nakagawa T, Kashiwagi H, Nakagawa H, Takeuchi S and Chung HY. In Vitro and in vivo studies on the radical-scavenging activity of tea [J]. Journal of Agricultural and Food Chemistry, 1998, 46:2143-2150.
[109] Bozkurt H. Utilization of natural antioxidants: Green tea extract and Thymbra spicata oil in Turkish dry-fermented sausage [J]. Meat Science, 2006, 73: 442–450.
[110] 毛 连 根 , 俞 洁 行 . 茶 叶 提 取 物 对 小 鼠 的 醒 酒 作 用 [J]. 茶 叶 科学,2000,20(2):155-157.
[111] 汤银红 ,丁斌如 .枳木具 子水提物对乙醇脱氢酶活性的影响 [J].中药药理与临床,2004,20(2):24.
[112] 任发政,罗云波,蒋菁莉.枳木具 子影响乙醇代谢机理研究[J].食品科学,2002,23(12):58-60.
[113] 伍嘉宁,潘锄云,周华楚,俞国荣 ,张佳英.葛花解醒汤抗乙醇中毒的研究[J].江西中医学院学报,1994,6(4):24-25.
[114] 陈正爱 ,于海玲 ,李美子 .解酒乐的解酒作用实验研究 [J].延边大学医学学报,2005,28(4):259-260.
[115] 汤 卫 东 , 朱 海 涛 . 醒 酒 保 健 口 服 液 的 研 制 [J]. 食 品 科技,2004,(6):75-76,79.
[116] Young JL, Caarol BP, Eugene JP. Effect of ginseng on plasma level of ethanol in the rat [J]. Planta Medica, 1993, 59:17-19.
[117] Kim YK, Guo Q, Packer L. Free radical scavenging activity of red ginseng aqueous extracts [J]. Toxicology, 2002, 172: 149–156.
[118] 李敬常,周伟贞,叶丽明,周保珍 ,解长跃.三七水提物对动物乙醇损伤的保护作用[J].中成药,1992,14(6):32-33.
[119] 白雪松 ,韩春姬 ,李克 .复方轮叶党参的醒酒作用实验研究 [J].延边大学医学学报,2004,27(3):166-169.
[120] 成霈 ,顾慧莹 ,宋敏 .赶黄草对预防醉酒及解酒效能研究 [J].医药世界,2006,5:142-144.
[121] 吴淑清 ,刘杰 ,牟莉 ,黄玉珍 .沙棘米醋对酒麻过程的影响研究[J].吉林粮食高等专科学校学报,2005,20(4):5-9.
[122] Masayuki Y, Emiko H, Hisashi M, Elatosides A and B.potent inhibitors of ethanol absorption in rats from A ralia elata Seem: the structure-activity relationships of oleanolic acid oligoglycosides [J]. Chem Pharm Bull, 1993, 41(11):2069-2071.
[123] Chung JH, Cheong JC and Park KS. Effect of aloin, a quinone derivative of A loe, on ethanol metabolism in the rat [J]. Medical Science Research, 1996, 24(6):421-423.
[124] Kiyoshi S, Toshiko Y and Yamane S. Effect of water extracts of crude drugs in decreasing blood ethanol concentrations in rats [J].Chem Pharm Bull, 1987, 35(11):4597-4604.
[125] Kiyoshi S, Yukiko S and Chie I. Effect of water extracts of A loe and some herbs in decreasing blood ethanol concentration in rats II [J].Chem Pharm Bull, 1989, 37(1):155-159.
[126] 王平.拐枣果浸渍液对机体乙醇代谢的影响[J].中南林学院学报,1997,17(3):65-67.
[127] 谭海荣 ,吴谦 ,潘竞锵 ,肖柳英 ,韩超 ,林洁茹 ,郑琳颖 ,李博萍 .水飞蓟宾对抗大鼠酒精性脂肪肝作用及机制[J].中国临床药理学与治疗学,2004,9(7):803-806.
[128] 新甫勇次郎,山崎津,中岛嘉次郎.葛花对乙醇诱发的代谢异常及 实 验 性 肝 损 伤 的 影 响 [J]. 国 外 医 药 : 植 物 药 分册,1991,6(5):226-227.
[129] 王庆端,江金花,孙文欣,夏薇.葛根总黄酮的抗啤酒中枢抑制作用[J].河南医科大学学报,1998,33(3):117-119.
[130] 张恩户,赵勤,梁作峰,杨文平.葛根总黄酮解酒作用的实验观察[J].陕西中医学院学报,1995,18(3):38-39.
[131] 苏德奇,马龙,周晓辉,杨浩峰,刘涛,田丽婷.醒酒保肝胶囊对急性 酒 精 性 肝 损 伤 的 影 响 [J]. 新 疆 医 科 大 学 学报,2005,28(5):396-398.
[132] 邓源 ,班春林 ,袁伯俊 .醒酒颗粒解酒作用机制的探讨 [J].解放军药学学报,2006,22(5):350-352.
[133] 宫德正,耿成燕,刘军 ,姜丽萍,仲来福.网状五层龙热水提取物醒酒作用的实验研究[J],毒理学杂志,2005,19(3):217-218.
[134] 黄永平,周世文,徐颖,汤建林,王莲,张津源,杨雪.乙酰半胱氨酸对小鼠酒精中毒的影响[J],中国药房,2004,15(7):403-404.
[135] 袁秉祥,孙晓东,罗笑宇,于晓江.永生宜康醒酒和减轻酒精致动物肝损伤药效学试验[J].酿酒科技,2005,(4):90-92.
[136] 傅兰,傅伟,沈伟,赵玉珍.白酒加入宜康后减轻对人体损伤的实验[J].酿酒,2004,31(1):85-86.
[137] 邓源,袁伯俊,张淑英.解酒灵对乙醇致鼠肝急性损伤的保护作用及机制的探讨[J].中国新药杂志,2000,9(6):388-391.
[138] 季光,曹承楼,张玮,邢练军,王奕,王育群.清肝活血方对酒精性肝 病 大 鼠 ADH 及 CYPⅡE1 的 影 响 [J]. 中 西 医 结 合 肝 病 杂志,2002,12(4):215-217.
[139] 周毅刚,徐绍锐,舒坤贤,文继舫 ,李昂.醒酒灵冲剂对机体内乙醇代谢的影响[J].湖南医学,2001,18(3):212,216.
[140] 周红宇,胡国新,胡爱萍,丁细桃 ,郑荣远.醒脑静注射液对乙醇所 致 记 忆 功 能 障 碍 的 影 响 [J]. 中 国 临 床 药 理 学 与 治 疗学,2004,9(1):110-112.
[141] 芦洁,李文哲,倪亚明.中药水提取物对乙醇脱氢酶活性影响的初步研究[J].同济大学学报(医学版),2002,23(1):23-24,30.
[142] Ganapathy V, Brandsch M and Leibach FH.Intestinal transport of amino acids and peptides [M]. In: Johnson LR ed, Physiology of the gastrointestinal tract, New York: Raven Press, 1994, 52:1773.
[143] Ehud Z, Moise B. Intestinal Absorption of peptides throught the enterocytes [J]. Microscopy Research and Technique, 2000, 49(4):346-352.
[144] Fei Y-J, Kanai Y, Nussberger S, Ganapathy V, Leibach FH, Romero MF, Singh SK, Boron WF and Hediger MA.Expression cloning of a mammaliam proton-coupled oligopeptide transporter [J]. Nature, 1994, 368:563-566.
[145] 陈历俊 ,程涛 ,骆承序 .肽基蛋白水解物及其生物活性 [J].食品工 业 科 技 ,1999, 增 刊 — 北 京 食 品 学 会 成 立 二 十 周 年 论 文集:178-182.
[146] 曾晓波,王海英,林永成.食物中的生物活性肽:生物活性及研究进展[J].食品工业科技,2004,25(4):151-155.
[147] Nagaoka S, Awano T, Nagata N, Masaoka M, Hori G and Hashimoto K. Serum cholesterol reduction and cholesterol absorption inhibition in Caco-2 cells by a soyprotein peptic hydrolyzate [J]. Bioscience, Biotechnology and Biochemistry, 1997, 61:354-356.
[148] Hughes J. Isolation of an endogenous compound from the brain with the pharmacological properties similar to morphine [J]. Brain Research, 1975, 88(2):295-308.
[149] Gill I, López-Fandi?o R, Jorba X and Vulfson EN. Biologically active peptides and enzymatic approaches to their production [J]. Enzyme and Microbial Technology, 1996, 18(3):162-183.
[150] Ferreira IMPLVO, Pinho O, Mota MV, Tavares P, Pereira A, Gon?alves MP, Torres D, Rocha C and Teixeira JA. Preparation of ingredients containing an ACE-inhibitory peptide by tryptic hydrolysis of whey protein concentrates [J].International Dairy Journal, 2007, 17(5):481-487.
[151] 杨浩峰 ,马龙 .乳源性生物活性肽研究进展 [J].乳业科学与技术,2006,(2):53-55.
[152] 王专 ,易华西 ,吴永英 .乳酪蛋白源生物活性肽的研究进展 [J].生物信息学,2006,4(2):95-97.
[153] Je J-Y, Park P-J, Kim S-K. Antioxidant activity of a peptide isolated from Alaska Pollack (Theragra chalcogramma) frame protein hydrolysate [J]. Food Research International, 2005, 38:45-50.
[154] Rajapakse N, Mendis E, Jung W-K, Je JY and Kim S-K. Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties [J]. Food Research International, 2005, 38:175-182.
[155] Yoshida T, Muto Y, Moriwaki H and Yamato M. Effect of long-term oral supplementation with branched-chain amino acid granules on the prognosis of liver cirrhosis [J]. Gastroenterologia Japonica, 1989, 24(6):692-698.
[156] Ravallec-Plé R, Charlot C, Pires C, Braga V, Batista I, Wormhoudt AV, Gal YL and Fouchereau-Péron M.The presence of bioactive peptides in hydrolysates prepared from processing waste of sadine (Sardina pichardus) [J]. Journal of the Science of Food and Agriculture, 2001, 81(11):1120- 1125.
[157] Jeon Y-J, Byun H-G and Kim S-K. Improvement of functional properties of codframe protein hydrolysates using ultrafiltration membranes [J]. Process Biochemistry, 1999, 35(5):471-478.
[158] Wu H C, Chen H-M and Shiau C-Y. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus) [J]. Food Research International, 2003, 36:949-957.
[159] Korhonen H, Pihlanto-Lepp?la A, Rantam?ki P and Tupasela T. Impact of processing on bioactive proteins and peptides [J]. Trendsin Food Science and Technology, 1998, 9(8-9):307- 319.
[160] 焦迎春,郑晓冬.活性肽在食品中的应用研究[J].粮油加工与食品机械,2002,(8):35-37.
[161] 张晓梅,钟芳,麻建国.大豆降胆固醇活性肽的初步分离纯化[J].食品与机械,2006,22(2):33-37.
[162] 陈 成 . 大 豆 蛋 白 活 性 肽 保 健 功 能 性 的 研 究 [J]. 大 豆 通报,2005,(2):22-24.
[163] 赵芳芳,张日俊.大豆肽研究进展[J].中国饲料,2004,(1):22-23.
[164] 荣建华 ,李小定 ,谢笔钧 .大豆肽体外抗氧化效果的研究 [J].食品科学,2002,23(11):118-120.
[165] 翟瑞文 ,李雁群 ,余世望 .用玉米渣生产玉米蛋白肽饮料 [J].食品科学,1997,18(9):31-33.
[166] Alexandra Alves-Rodrigues and Andrew Shao. The science behind lutein [J]. Toxicology Letters, 2004, 150(1):57-83.
[167] 周大寨 ,黄国清 ,唐巧玉 .用 HPLC测定酶解玉米蛋白氨基酸的组成[J].食品科学,2005,26(2):179-181.
[168] 王梅,谷文英.酶解玉米黄粉蛋白制备可溶性肽[J].粮油食品科技,1999,17(1):1-5.
[169] 金英姿,庞彩霞.碱性蛋白酶水解玉米蛋白工艺条件的研究[J].食品研究与开发,2006,27(3):44-45.
[170] 崔 东 善 . 蛋 白 酶 及 其 在 食 品 中 的 应 用 [J]. 杭 州 食 品 科技,1997,44(1):15-19,22.
[171] 张晓峰 ,陈庆森 ,庞广昌 .酶解 100kg玉米蛋白粉制备玉米功能短肽的中试研究[J].食品工业科技,2006,(7):58-59.
[172] Peter RS and Nigel GH. Cereal seed storage proteins: structures, properties and role in grain utilization [J]. Journal of Experimental Botany, 2002, 53:947-958.
[173] 鲁晓翔,陈新华,唐津忠.酶法改性玉米蛋白功能特性的研究[J].食品科学,2000,21(12):13-15.
[174] 佐 野 良 史 . コ ㄧ ン ぺ チ ド の 食欠 料 へ の 利 用 [J]. 食 品 と 科学,1993,2:102-106.
[175] 陈 文 , 陈 代 文 . 玉 米 蛋 白 粉 的 饲 用 价 值 及 鉴 别 [J]. 饲 料 博览,2003,(9):29-30.
[176] 张艳铭 ,甄二英 ,宋智娟 .玉米蛋白粉的营养价值及其应用 [J].当代畜牧,2006,(1):22-24.
[177] 郭亮,安秀芳,李淑英,郭帅.玉米蛋白粉营养价值评定及其评定技术的研究[J].中国畜牧兽医,2004,31(3):16-18.
[178] 王 玉 军 , 单 立 志 . 玉 米 蛋 白 在 食 品 中 的 应 用 [J]. 黑 龙 江 粮食,2003,(3):35-36.
[179] 李大婧,杨同舟,季亮,王玉军.玉米蛋白粉在食品中的应用研究[J].粮油食品科技,2001,9(1):12-14.
[180] 江洪波,左艳文.玉米蛋白发泡粉的生产工艺[J].长江大学学报(自然版),2006,3(2):188-190.
[181] 江洪波,左艳文.玉米蛋白发泡粉生产工艺的研究[J].安徽农学通报,2006,12(6):210-211.
[182] 昌 友权 ,郑鸿雁 ,张建峰 ,陈立阁 ,程宏 .精制玉米蛋白粉包埋DHA的研究[J].吉林农业大学学报,2001,23(1):107-110.
[183] 李晶,张丽萍.精制玉米蛋白粉在DHA中的应用研究[J].食品工业科技,2004,25(8):67-69.
[184] 王学智,任桂久,吴之传,谢同云,耿传,丁胜利.研制玉米蛋白基生 物 降 解 性 塑 料 的 初 步 结 果 [J]. 安 徽 机 电 学 院 学报,2000,15(1):10-14.
[185] 王学智.玉米蛋白基生物降解性塑料的初步研究[J].实验与创新思维,2004,(12):17-19.
[186] 胡 学 烟 , 孙 冀 平 . 玉 米 蛋 白 粉 开 发 与 利 用 [J]. 粮 食 与 油脂,2001,(11):32-33.
[187] 孙震,苏宇杰,姚惠源.玉米蛋白粉中黄体素、玉米黄素对乳腺癌细胞增殖的影响[J].食品与机械,2005,21(2):6-11.
[188] 王薇,韩鲁佳.玉米蛋白粉中叶黄素提取及皂化工艺的研究[J].中国农业大学学报,2006,11(4):125-130.
[189] 李 勇 , 赵 杰 文 . 玉 米 黄 色 素 的 提 取 纯 化 研 究 [J]. 中 国 粮 油 学报,2006,21(3):74-77.
[190] 孙震,李博,姚惠源.玉米蛋白粉中叶黄素和玉米黄素对口腔癌细胞DNA的损伤作用[J].食品科技,2006,(4):131-134.
[191] 孙震,奚海燕,陈正行,姚惠源.玉米蛋白粉中叶黄素和玉米黄素对 小 鼠 巨 噬 细 胞 免 疫 调 节 活 性 的 研 究 [J]. 食 品 科学,2006,27(11):505-509.
[192] 孙震,姚惠源.玉米蛋白粉中玉米黄素对人口腔鳞癌细胞株KB增殖的抑制[J].食品与生物技术学报,2005,24(6):34-37.
[193] 程谦伟,郭兴凤,熊拯.玉米的综合开发—醇溶蛋白的提取[J].粮食科技与经济,2006,(6):43-45.
[194] 刘志国 ,胡丽丽 ,史鑫 .玉米醇溶蛋白的制备 [J].武汉食品工业学院学报,1997,(3):6-9.
[195] 姚晓敏,孙向军,卢杰.可食性玉米醇溶蛋白成膜工艺的研究[J].食品工业科技,2002,23(1):20-23.
[196] 张钟,徐丽红、桑红庆.玉米营养保健口香糖的研制[J].食品工业,2004,(6):12-13.
[197] 李文静,田崇明,张永申,胡卫国.玉米醇溶蛋白在糖果中的应用研究[J].食品工业科技,2002,23(5):31-32.
[198] 田崇明,李文静,胡卫国.玉米醇溶蛋白在香蕉保鲜中的应用研究[J].食品科技,2002,(3):66,69.
[199] 庞 彩 霞 , 金 英 姿 . 玉 米 醇 溶 蛋 白 的 应 用 [J]. 内 蒙 古 农 业 科技,2005,(4):40-41.
[200] Olsman H. Hydrolyzed and autolyzed vegetable proteins as functional food ingredients [J]. Journal of the American Oil Chemists Society, 1979, 56:375-376.
[201] 郭瑶,曾名勇,崔文萱.水产胶原蛋白及胶原多肽的研究进展[J].水产科学,2006,25(2):101-104.
[202] 曾名勇,崔海英,李八方.海洋生物活性肽及其生物活性研究进展[J].中国海洋药物杂志,2005,24(1):46-51.
[203] 刘云国,李八方,汪东风,赵雪,林琳,冯大伟,毕琳.海洋生物活性肽研究进展[J].中国海洋药物杂志,2005,24(3):52-57.
[204] 翟瑞文,李雁群,陈子林,余世望.玉米肽的制备特性与应用[J].食品工业,1998,3:11-14.
[205] 叶启腾 ,陈强 .木瓜蛋白酶的应用 [J].广西热作科技 ,1999 (4): 34-35.
[206] 王遂,尤旭.微生物蛋白酶对玉米皮蛋白水解作用的研究[J].食品工业科技,2000,21(4):22-24.
[207] 李艳丽,陈光,丛建民,孙扬,刘艳秋,吴歌.碱性蛋白酶水解法制备玉米肽的研究[J].吉林农业大学学报,2004,26(2):142-144,149.
[208] Xu L, Wu X-x, Fei X-f, Zhang L-q and Zhang X-z. A study on bioactivity of corn peptides with low molecular weight Ⅰ: Effect of intake of them on alcohol metabolism in rats [J]. Chemical Research in Chinese Universities, 2003, 19(2):180-182.
[209] Xu L, Zhang L-q, Wu X-x, Wang N and Zhang X-z. A study of bioactivity of corn peptides with low molecular weight Ⅱ: Effect on plasma free amino acid concentrations in rats [J]. Chemical Research in Chinese Universities, 2003, 19(3):314-316.
[210] 刘志国,陈江源,王亚林,刘生毅.玉米醇溶蛋白酶解制备ACE抑制肽的研究[J].食品研究与开发,2006,27(2):138-141.
[211] 崔凌飞 ,王遂 .Alcalase蛋白酶水解玉米皮蛋白的研究 [J].哈尔滨师范大学自然科学学报,2002,18(1):62-66.
[212] 韩继福,任建波.Alcalase酶水解玉米蛋白粉制备可溶性肽最佳条件的研究[J].吉林农业科学,2003,28(3):46-49.
[213] 陈新,陈庆森,庞广昌.不同变性方式对Alcalase AF214L酶解玉米蛋白水解度的影响[J].食品与发酵工业,2005,31(2):42-44.
[214] 邓涛,刘利锋.玉米蛋白水解液在温度敏感型菌种中的尝试[J].发酵科技通讯,2006,35(2):29-30.
[215] 杨剑 ,卢昌义 ,于兴娜 .4种玉米水解肽的除草活性及与相对分子质量的关系[J].生态与农村环境学报,2006,22(3):33-36.
[216] 郑喜群,李里特,吴红艳,刘晓兰.利用米曲霉羧肽酶由玉米蛋白粉制备高F值寡肽[J].食品科学,2004,25(增刊),43-46.
[217] 李 峰 , 徐 军 庆 . 高 F 值 寡 肽 的 研 究 与 开 发 [J]. 食 品 与 药品,2005,7(1A):43-44.
[218] 谷文英,过世东,沈培英,王梅.酶解玉米蛋白粉制备高F值寡肽[J].精细与专用化学品,2001,(21):22-23.
[219] 王晓杰,郑喜群,刘晓兰,林杰,王丽丽,李里特.利用纳豆杆菌发酵玉米蛋白粉新工艺研究[J].食品研究与开发,2006,27(5):42-45.
[220] 何慧,谢笔钧,杨卓,唐谊练.大豆蛋白和玉米蛋白酶解肽及其活性研究[J].粮油食品科技,2002,10(1):14-16.
[221] Hardwick JE and Glatz CE. Enzymatic Hydrolysis of Corn Gluten Meal [J]. Journal of Agricultural and Food Chemistry, 1989, 37:1188-1192.
[222] 李升福,闻海波.玉米蛋白水解物的功能性—抗疲劳的研究[J].食品科学,2006,27(08):245-247.
[223] 昌 友 权 . 玉 米 肽 抗 疲 劳 作 用 的 实 验 研 究 [J]. 食 品 科学,2004,25(9):173-178.
[224] 李 晶 . 玉 米 肽 抗 疲 劳 作 用 的 实 验 研 究 [J]. 食 品 与 机械,2004,20(1):11-12,27.
[225] Mills ENC, Alcocer MJC and Morgan MRA. Biochemical interactions of food-derived peptides [J]. Trends in Food Science and Technology, 1992, 3:64-68.
[226] Liu DL-Y, Christians NE and Garbutt JT. Herbicidal activityof hydrolyzed corn gluten meal on three grass species under controlled environments [J]. Journal of Plant Growth Regulation,1994, 13(4):221-226.
[227] Liu DL-Y and Christians NE. Isolation and identification of root-inhibiting compounds from corn gluten hydrolysate [J]. Journal of Plant Growth Regulation, 1994, 13(4):227-230.
[228] Yamaguchi M, Takada M, Nozaki O, Ito M and Furukawa Y. Preparation of corn peptides from corn gluten meal and its administration effect on alcohol metabolism in stroke-prone spone spontaneously hypertensive rats [J]. Journal of Nutritional Science and Vitaminology, 1996, 42:219-231.
[229] Yamaguchi M, Nishikiori F, Ito M and Furukawa Y. The effects of corn peptide ingestion on facilitating alcohol metabolism in healthy men [J]. Bioscience Biotechnology and Biochemistry, 1997, 61(9):1474-1481.
[230] 徐力,刘立,李相鲁,乔岩梅,吴晓霞,张学忠.玉米功能短肽的制备 及 其 类 超 氧 化 物 歧 化 酶 活 性 研 究 [J]. 中 国 生 化 药 物 杂志,2002,23(2):78-80.
[231] 徐力 ,李相鲁 ,黄宜兵 ,吴晓霞 ,候瑞珍 ,王华 ,王娜 ,张学忠 .小分子玉米肽Leu-Asp-Tyr-Glu保护线粒体抗氧化损伤的研究[J].高等学校化学学报,2004,25(6):1073-1075.
[232] 孙红,于得伟,崔志勇,杨明.玉米肽对动物实验性肝损伤的保护作用[J].中药药理与临床,2002,18(3):10-111.
[233] Adamson N J and Reynolds EC. Characterization of casein phosphopeptides prepared using alcalase: Determination of enzyme specificity [J]. Enzyme and Microbial Technology, 1996, 19:202-207.
[234] 黄伟坤.食品检验与分析[M].北京:中国轻工业出版社: 1989,第1版,23-25.
[235] 王文侠.超临界二氧化碳萃取米糠油的生产工艺研究[J].工艺技术,2005,26(8):107-112.
[236] 王红菊,郭维静,郭洪臣,班玉凤,陈黎行,王祥生.一种提高玉米蛋白水解度和降解率的方法[J].食品科技,2005,(6):92-95.
[237] 郭维静,权文吉,陈黎行,郭洪臣 ,王祥生.玉米蛋白粉的剪切乳化 处 理 及 酸 性 蛋 白 酶 的 水 解 研 究 [J]. 食 品 工 业 科技,2006,27(10):103-105.
[238] Kong B and Xiong YL. Antioxidant activity of zein hydrolysates in a liposome system and the possible mode of action [J]. Journal of Agricultural and Food Chemistry, 2006, 54:6059–6068.
[239] 李自升 ,蔡木易 ,易维学 .玉米蛋白粉的酶解工艺初探 [J].食品与发酵工业,2006,32(4):67-69.
[240] 隋玉杰,何慧,王进,贺菊萍,胡筱波.中性蛋白酶及碱性蛋白酶制 备 玉 米 粗 肽 的 醒 酒 活 性 比 较 [J]. 中 国 粮 油 学报,2006,21(3):102-106.
[241] 郑冬梅 ,李升福 ,孔保华 .玉米蛋白水解条件的优化研究 [J].食品科学.2002,23(8):52-56.
[242] 许宗运,马少宾,张秀萍,赵卫国,刘利林,吕银龙.DPPH法评价37种植物抗氧化活性[J].塔里木农恳大学学报,2004,16(2):1-4.
[243] Milardovié S, Ivekovic D and Grabaric BS.A novel amperometric method for antioxidant activity determination using DPPH free radical [J]. Bioelectrochemistry, 2006, 68:175–180.
[244] Marklund S and Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase [J]. European Journal of Biochemistry, 1974, 47:469-474.
[245] 吕永为,郭详群.荧光分光光度法测定两种中药对羟自由基的清除作用[J].厦门大学学报(自然科学版),2004,43(2):208-212.
[246] 杨 祥 宇 , 宋 健 , 冯 荣 秀 . 荧 光 标 记 染 料 [J]. 化 学 通报,2003,66(9):615～621.
[247] Yamaguchi M, Nishikiori F, Nozaki O, Ito M and Furukawa Y. Effcet of long-team ‘corn peptide’ ingestion on alcohol metablism in stroke-prone spontaneously hypertensiverats with alcohol loading [J]. Journal of Nutritional Science and Vitaminology, 1996, 42:567-580.
[248] Yamaguchi M, Nishikiori F, Ito M and Furukawa Y. Effcet of corn peptide on alcohol metabolism and plasma free amino acid concentrations in healthy men [J]. European Journal of Clinical Nutrition, 1996, 50:682-688.
[249] [德]B.施特尔马赫著,钱嘉渊译.酶的测定方法[M].中国轻工业出版社:1992,第1版,17-19.
[250] 陈灏珠.实用内科学(上册)[M].北京:人民卫生出版社,2003,第11版:789-790.
[251] Young IS and McEneny J. Lipoprotein oxidation and atherosclerosis [J]. Biochemical Society Transactions, 2001, 29(Part 2):358-362.
[252] 方 允 中 , 杨 胜 , 伍 国 耀 . 自 由 基 稳 衡 性 动 态 [J]. 生 理 科 学 进展,2004,35(3):199-204.
[253] Gebicki JM. Comparison of the capacities of the perhydroxyl and the superxide radicals to initiate chain oxidation of linolieic acid [J]. American Chemical Society, 1981, 103(23):7020-7022.
[254] Mak IT, Kramer JK and Weglicki WB. Potention of free radical-induced lipid peroxidative injury to sarcolemmal membranes by lipid amphiphiles [J]. Journal of Biological Chemistry, 1986, 261:1153-1157.
[255] Chung AS and Maines MD. Effect of selenium on glutathione metabolism: Induction of γ-glutamylcysteine synthetase and glutathione reductase in the rat liver [J]. Biochemical Pharmacology, 1981, 30(23):3217-3223.
[256] Hodgson EK and Fridovich L. The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide. Inactivation of the enzyme [J]. Biochemistry, 1975, 14(24):5294-5299.
[257] Zloch Z. Oxidation/antioxidation status after an acute ethanol intoxication [J]. Toxicology Letters, 1995, 78(supplement 1):87.
[258] ?ner-yidogan Y, Gürd?l F and ?ner P. The effects of acute melatonin and ethanol treatment on antioxidant enzyme activities in rat testes [J]. Pharmacological Research, 2001, 44(2):89-93.
[259] Vendemiale G, Grattagliano I, Signorile A and Altomare E. Ethanol-induced changes of intracellular thiol compartmentation and protein redox status in the rat liver: effect of tauroursodeoxycholate [J]. Journal of Hepatology, 1998, 28(1):46-53.
[260] Yamaguchi M, Takeuchi M, Ebihara K. Inhibitory effect of peptide prepared from corn gluten meal on 7,12-Dimethylbenz anthracene-induced mammary tumor progression in rats [J]. Nutrition Research, 1997, 17(7):1121-1130.
[261] 张权义,欧阳云.多样品减重法测定乳制品中脂肪含量实验分析[J].实用预防医学,2004,11(2):279-280.
[262] Jens Adler-Nissen. Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid [J]. Journal of Agricultural and Food Chemistry, 1979, 27(6):1256-1262.
[263] 张龙翔,张庭芳,李令媛主编.生化实验方法和技术[M].北京:高等教育出版社,1997,第1版,137-139.
[264] Chen S-T, Chen S-Y, Wang K-T. Kineticaly controlled peptide bond formation in anhyolrons alcohol catalyzed by the industrial protease Alcalase [J]. Journal of Organic Chemistry, 1992,57:6960-6965.
[265] Hojilla-Evangelista MP and Johnson LA. Optimizing extraction of zein and glutelin-rich fraction during sequential extraction processing of corn [J]. Cereal Chemistry, 2003, 80(4):481-484.
[266] Lawton JW. Zein:A history of processing and use [J].Cereal Chemistry, 2002, 79(1):1-18.
[267] 黄国平,温其标.超声波法提取玉米醇溶蛋白的研究[J].食品与发酵工业,2002,28(10):1-5.
[268] 张秋荣,吴春丽,单丽红,刘艳凯 ,李良辰.玉米蛋白粉中醇溶蛋白 超 声 萃 取 工 艺 改 进 [J]. 郑 州 大 学 学 报 ( 医 学版),2005,40(5):913-915.
[269] 张钟,齐爱云.玉米醇溶蛋白提取工艺及功能性质研究[J].粮食与饲料工业,2004,(9):21-23.
[270] Mannheim A and Cheryan M. Water-soluble zein by enzymatic modification in organic solvents [J].Cereal Chemistry, 1993, 70(2):115-121.
[271] 徐力,李相鲁,吴晓霞,王华,候瑞珍,黄宜兵,张学忠.一种新的玉米 抗 氧 化 肽 的 制 备 与 结 构 表 征 [J]. 高 等 学 校 化 学 学报,2004,25(3):466-469.
[272] Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. Protein measurement with the Folin phenol reagent [J]. The Journal of Biological Chemistry, 1951, 193:265-275.
[273] Summer JP, Sisler EB. A simple method for blood sugar [J]. Archives of Biochemistry and Biophysics, 1944, 4:333.
[274] Miller GL. Use of dinitrosalicylie acid reagent for determination of reducing sugar [J]. Analytical Chemistry, 1959, 31(3):426-428.
[275] 王镜岩、朱圣庚、徐长法.生物化学(上册)[M],高等教育出版社:2003,第3版,103.
[276] Ozcelik B, Lee JH and Min DB. Effects of light, oxygen and pH on the absorbance of 2,2-diphenyl-1-picrylhydrazyl [J]. Journal of Food Science, 2003, 68(2):487–490.
[277] Yamazaki E, Inagaki M, Kurita O and Inoue T. Antioxidant activity of Japanese pepper (Zanthosylum piperitum DC.) fruit [J]. Food Chemistry, 2007, 100(4):171-177.
[278] Shimada K, Fujikawa K, Yahara K and Nakamurat T. Antioxidative properties of xanthan on the antioxidation of soybean oil in cyclodextrin emulsion [J]. Journalof Agricultural and Food Chemistry, 1992, 40:945-948.
[279] Cotelle N, Bernier J-L, Catteau J-P, Pommery J, Wallet J-C and Gaydou EM. Antioxidant Properties of Hydroxy-Flavones [J]. Free Radical Biology and Medicine, 1996, 20(1):35-43.
[280] Hu F, Schmidt K, Stefka S, Li Z, Udo G, Matthias H. Radical scavengers from the entomogenous deuteromycete beauveria amorpha [J]. Planta Medica, 2002, 68:64～65.
[281] 李永利,张焱.邻苯三酚自氧化法测定SOD活性[J].中国卫生检验杂志,2000,10(6):673.
[282] 张宏,谭竹钧.四种邻苯三酚自氧化法测定超氧化物歧化酶活性 方 法 的 比 较 [J]. 内 蒙 古 大 学 学 报 ( 自 然 科 学版),2002,33(6):677-681.
[283] 李建武,萧能庚,余瑞元等.生物化学实验原理和方法[M].北京大学出版社:1997,第1版,408.
[284] 翟继英.光度法测定羟自由基及蔬菜抗氧化性[J].卫生职业教育,2005,23(7):107-108.
[285] Ranathunga S, Rajapakse N and Kim S-K. Purification and characterization of antioxidative peptide derived from muscle of conger eel (Conger myriaster) [J]. European Food Research and Technology, 2006, 222(3-4):310-315.
[286] Oyaizu M. Antioxidative activities of browning products of glucosaming fractionated by organic solvent and thin-layer chrlmatography [J]. Nippon Shokuhin Kogyo Gakkaishi, 1988, 35:771-775.
[287] Haseba T, Tomita Y, Kurosu M and Ohno Y. Dose and time changes in liver alcohol dehydrogenase (ADH) activity during acute alcohol intoxication involve not only class I but also class III ADH and govern elimination rate of blood ethanol [J]. Legal Medicine, 2003, 5:202-211.
[288] Li SY, Gomelsky M, Duan J, Zhang Z, Gomelsky L, Zhang X, Epstein PN and Ren J. Overexpression of aldehyde dehydrogenase-2 (ALDH2) transgene prevents acetaldehyde-induced cell injury in human umbilical vein endothelial cells [J]. The Journal of biological chemistry, 2004, 279(12):11244-11252.
[289] Moon K-H, Kim B-J, Song BJ. Inhibition of mitochondrial aldehyde dehydrogenase by nitric oxide-mediated S-nitrosylation [J]. FEBS Letters, 2005, 579:6115–6120.
[290] Chrostek L, Szmitkowski M and Jelski W. Alcohol and aldehyde dehydrogenase activity measured with fluorogenic substrates in the liver of rats poisoned with methanol [J]. Experimental Toxicologic Pathology, 2001, 53:77-80.
[291] Mezey E, Rennie-Tankersley L and Potter JJ. Effect of leptin on liver alcohol dehydrogenase [J]. Biochemical and Biophysical Research Communications, 2005, 337:1324-1329.
[292] 邢其毅,徐瑞秋,周政,裴伟伟.基础有机化学[M].高等教育出版社: 2000,第1版,454-459.
[293] Conduah Birt JEE, Shuker DEG and Farmer PB. Stable acetaldehyde-protein adducts as biomarkers of alcohol exposure[J]. Chemical Research in Toxicology, 1998, 11:136-142.
[294] Klassen LW, Jones BL, Sorrell MF, Tuma DJ. Thiele GM. Conversion of acetaldehyde-protein Adduct epitopes from a nonreduced to a reduced phenotype by antigen processing cells [J]. Alcoholism: Clinical and Experimental Research, 1999, 23(4):657-663.
[295] Thiele GM Miller JA, Klassen LW and Tuma DJ. Long-term ethanol administration alters the degradation of acetaldehyde adducts by liver endothelial cells [J]. Hepatology, 1996, 24(3):643-648.
[296] 金宗濂,文镜,唐粉芳 ,陈文.功能食品评价原理及方法[M]. 北京大学出版社:第1版,1995,10.
[297] 司 徒 镇 强 , 吴 军 正 . 细 胞 培 养 [M]. 世 界 图 书 出 版 社 :( 修 订版)2005,251-252.
[298] 孙素兰,俞励平,文洁 ,叶青山,朱建梅.灵芝片抗疲劳作用实验研究[J].食品与药品,2006,8(6A):44-46.
[299] 刘静波,林松毅,程胜.主成分分析方法综合评价功能食品抗疲劳和耐缺氧功效特性[J].中国食品学报,2006,6(1):212-217.
[300] Park K-J and Hyun C-K. Antigenotoxic effects of the peptides derived from bovine blood plasma proteins [J]. Enzyme and Microbial Technology, 2002, 30(5):633- 638.
[301] Monti JC and Jost R. Enzymatic solubilization of heat-denatured cheese whey protein [J]. Journal of Dairy Science, 1978, 61:1233-1237.
[302] 付清泉,左斌海,李天全,万昌秀.几类生物活性肽的研究进展[J]航天医学与医学工程,2002,15(3):227-230.
[2] Ji LL. Antioxidants and oxidative stress in exercise [J]. Proceedings of the Society for Experimental Biology and Medicine, 1999, 222 (3):283-292.
[3] 郑荣梁.自由基生物学[M].高等教育出版社:1992,第1版,21.
[4] Shinohara R, Mano T, Nagasaka A, Hayashi R, Uchimura K, Nakano I, Watanabe F, Tsugawa T, Makino M, Kakizawa H, Nagata M, Iwase K, Ishizuki Y and Itoh M. Lipid peroxidation levels in rat cardiac muscle are affected by age and thyroid status [J]. Journal of Endocrinology, 2000, 164 (1):97-102.
[5] 赵克然 ,杨毅军 ,曹道俊 .氧自由基与临床 [M].中国医药科技出版社:2000,第1版,9-10.
[6] Svingen BA, Neal FO and Aust SD. The role of superoxide and singlet oxygen in lipid peroxidation [J]. Photochemistry and Photobiology, 1978, 28 (4-5):803-809.
[7] Boveris A, Cadenas E. Production of superoxide radicals and hydrogen peroxide in mitochondria [M]. Oberley LW ed, Superoxide Dismutase. Florida: CRC Press, 1982, 2:15-30.
[8] Fridovich I. Measuring the activity of superoxide dismutase: An emvarrassment of riches [M]. In: Oberley LW ed, Superoxide Dismutase. Florida: CRC Press, 1982, 2:69-78.
[9] Bors W. In current topics in radiation research quarterly [M]. Armsterdam: North-Holland, Florida, 1974, 9:247.
[10] Ohnishi T, Blum H, Salerno JC. In function of quinones in energy-conserving systems [M]. Trumpower BL ed, New York: Academic Press, 1980.
[11] Halliwell B and Gutteridge JMC. Free radicals in biology andmedicine [M]. Oxford Clarendon Press: 1989, 110.
[12] Bors W, Saran M and Czapski G. The nature of intermediates during biological oxygen activation [M]. In: Bannister WH, Bannister JW ed, Biological and clinical aspects of superoxide and superoxide dismutase, 1980, 1-31.
[13] Cohen G and Cederbrum AI. Chemical evidence for production of hydroxyl radicals during microsomal electron transfer [J]. Science, 1979, 204:66-68.
[14] Bors W, Saran M, Michel C. Assay of oxygen radical method and mechanisms [M]. Obeley LW ed, Superoxide Dismutase Floreda: CRC Press, 1989, 31-62.
[15] Ekstr?m G, Cronholm T and Ingelman-Sundberg M. Hydroxyl-radical production and ethanol oxidation by liver microsomes isolated from ethanol-treated rat [J]. Biochemical Journal, 1986, 233:755-761.
[16] Fridovich I. Free radicals in biology [M]. Pryor WA ed, New York: Academic Press, 1976, 1:239.
[17] Kanofsky JR. Singlet oxygen production in superoxide ion-halocarbon systems [J]. Journal of the American Chemical Society, 1986, 108(11):2977-2979.
[18] 赵 保 路 . 氧 自 由 基 和 天 然 抗 氧 化 剂 [M]. 科 学 出 版 社 :2002, 第 1版,77-83.
[19] 赵燕 ,江文 ,侯京武 ,赵保路 ,文娟 .钙过负荷和丹参酮对线粒体脂质 过 氧 化 的 影 响 [J]. 生 物 化 学 与 生 物 物 理 学 报 ( 英 文版),1995,27(6):610-615.
[20] 赵 保 路 . 肿 瘤 细 胞 膜 的 流 动 性 [J]. 国 外 医 学 分 子 生 物 学 分册,1984,6(5):215-218.
[21] Nikola Getoff. Factors influencing the efficiency of radiationinduced degradation of water pollutants [J]. RadiationPhysics and Chemistry, 2002, 65(4-5):437-446.
[22] 郑 建 仙 .功 能 性 食 品 ( 1卷 ) [M].中 国 轻 工 业 出 版 社 :1999,第 1版,234-247.
[23] Pryor W A. Free radical biology: xenobiotics, cancer and aging [J]. Annals of the New York Academy of Sciences, 1982, 393(1):1-22.
[24] Lomnitski L, Bergman M, Schon I and Grossman S. The effect of dietary vitamin E and β-carotene on oxidantion processes in the rat testis [J]. Biochemical et Biophysical Acta, 1991, 1082(1):101-107.
[25] 崔剑,李兆龙,洪啸吟.自由基生物抗氧化与疾病[J].清华大学学报(自然科学版),2000,40(6):9-12.
[26] Hass HM and Massaro D. Differences in Cu, Zn superoxide dismutase induction in lungs of neonatal and adult rats [J]. American Journal of Physiology Cell Physiology, 1987, 253:66-70.
[27] Natvig DO, Imlay K, Touati D and Hallewell RA. Human copper-zinc superoxide dismutase complements superoxide dismutase-deficient Escherichia coli mutants [J]. Journal of Biological Chemistry, 1987, 262(30):14697-14701.
[28] Kirkman HN, Galiano S, Gaetani GF. The function of catalase-bound NADPH [J]. Journal of Biological Chemistry, 1987, 262(2):660-666.
[29] Anonymous A. A termination codon specifies selenocysteine in glutathione peroxidase [J]. Nutrition Reviews, 1988, 46:202-230.
[30] Anisimov VN, Zavarzain NY. Melatonin increases both life span and tumor incidence in female CBA mice [J]. Journal of Gerontology Series A-Biological Sciences and Medical Sciences, 2001, 56(7):311-323.
[31] Kopánia M, Celec P, Danixovi L, Michalka P and Biró C. Oxidative stress and electron spin resonance [J]. Clinica ChimicaActa, 2006, 364:61–66.
[32] Kong J-M, Li L-Z, Xie K-S and Wu W-K. Antioxidant capacity measurement of flavonoids by chemil uminescence method [J]. Natural Product Research and Development, 2004, 16(1):46-49.
[33] Laurent D, Marie-Héléne L and Avishay-Abraham S. High-performance liquid chromatographic assay of hydroxyl free radical using salicylic acid hydroxylation during in vitro experiments involving thiols [J]. Journal of Chromatography B, 2001, 763:185-193.
[34] 郑雄敏,徐旭士.茶多酚对豆油及菜油抗氧化性的研究[J].南昌大学学报(理科版),1998,22(2):105-108.
[35] 韩丽华,王丽红,范希王月.大豆异黄酮抗氧化性的研究 [J].中国油脂,2001,26(6):41-44.
[36] 魏安池,周瑞宝,瞿水忠.诃子抗氧化性能的研究[J].郑州粮食学院学报,1998,19(1):8-12.
[37] 王文中,王颖.迷迭香的研究及其应用—抗氧化剂[J].中国食品添加剂,2002,(5):60-65.
[38] 金鸣,蔡亚欣,李金荣,赵辉.邻二氮菲-Fe2+氧化法检测H2O2/Fe2+产 生 的 羟 自 由 基 [J]. 生 物 化 学 与 生 物 物 理 进展,1996,23(6):553-555.
[39] 贾之慎,邬建敏,唐孟成.比色法测定Fenton反应产生的羟自由基[J].生物化学与生物物理进展,1996,23(2):184-186.
[40] 于守洋,崔鸿斌,石华.中国营养保健食品指南[M].黑龙江科学技术出版社:1996,第1版,50-51.
[41] Delgado-Zamarre?o MM, González-Maza I, Sánchez-Pérez A and Carabias Martínez R. Analysis of synthetic phenolic antioxidants in edible oils by micellar electrokinetic capillary chromatography [J]. Food Chemistry, 2007, 100:1722–1727.
[42] Jun S-Y, Park P-J, Jung W-K and Kim S-K. Purification andcharacterization of an antioxidative peptide from enzymatic hydrolysate of yellowfin sole (Limanda aspera) frame protein [J]. European Food Research and Technology, 2004, 219:20-26.
[43] 佘世望,肖小年,范青生,吴凌伟,贺新兴,詹志军.60种药食两用植物抗氧化作用研究[J].食品科学,1995,16(11): 3-5.
[44] Uthrie N, Kurowska EMN. Anticancer and cholesterol-lowering activities of citrus flavonoids [M].Handbook of Nutraceuticals and Functinoal Foods. CRC Press: Boca Raton, Florida, 2001, 113-126.
[45] Du Toit R, Volsteedt Y and Apostolides Z. Comparison of the antioxidant content of fruits, vegetables and teas measured as vitamin C equivalents [J]. Toxicology, 2001, 166(1-2):63-69.
[46] Kim S-K, Kim Y-T, Byun H-G, Nam K-S, Joo D-S and Shahidi F. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin [J]. Journal of Agricultural and Food Chemistry, 2001, 49(4):1984–1989.
[47] Thiansilakul Y, Benjakul S and Shahidi F, Compositions, functional properties and antioxidative activity of protein hydrolysates prepared from round scad (Decapterus maruadsi)[J]. Food chemistry, 2007, 103:1385-1394.
[48] Klompong V, Benjakul S, Kantachote D and Shahidi F. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type [J]. Food Chemistry, 2007, 102:1317-1327.
[49] Velloglu YS, Mazza G, Gao L and Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products [J]. Journal of Agricultural and Food Chemistry, 1998, 46: 4113-4117.
[50] 丁利君,周燕芳,林楠.溪黄草中黄酮类物质提取及对羟自由基清除作用[J].食品与机械,2004,20(3):13-14.
[51] 蔡为荣.微波提取荷叶黄酮及其清除羟自由基的研究[J].食品科学,2004,25(9):112-115.
[52] 赵瑞艳,徐雅琴.树莓叶片中黄酮类物质提取及抗氧化性研究[J].食品科学,2006,22(2):104-106.
[53] 杨锦华,李博,籍保平.我国常见食用和药用植物的抗氧化性研究[J].食品科学,2006,27(6):87-91.
[54] Wang J-S, Zhao M-M, Zhao Q-Z and Jiang Y-M. Antioxidant properties of papain hydrolysates of wheat gluten in different oxidation systems [J]. Food Chemistry, 2007, 101:1658-1663.
[55] Zhu K, Zhou H. and Qian H. Antioxidant and free raical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase [J]. Process Biochemistry, 2006, 41:1296-1302.
[56] Gibbs BF, Zougman A, Masse R and Mulligan C. Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food [J]. Food Research International, 2004, 37:123-131.
[57] Rüfer CE and Kulling SE. Antioxidant activity of isoflavones and their major metabolites using different in vitro assays [J]. Journal of Agricultural and Food Chemistry, 2006, 54:2926-2931.
[58] 韩立强,朱松波,杨国宇.肌肽—一种有待开发的天然抗氧化剂[J].中国畜牧杂志,2005,41(2):33-35.
[59] 聂少平,谢明勇,罗珍.茶叶多糖的抗氧化活性研究[J].天然产物研究与开发,2005,17(5):549-552.
[60] 胡庭俊 ,程富胜 ,陈炅然 ,梁纪兰 ,董鹏程 .山豆根多糖体外清除自由基作用的研究[J].中兽医医药杂志,2004,(5):6-8.
[61] 王辉,罗顺德,刘刚,陈伟.莲心碱对氧自由基和脂质过氧化的影响[J].中国药理学通报,2004,20(10):1197-1198.
[62] 李丹 ,肖刚 ,丁霄霖 .苦荞黄酮清除自由基作用的研究 [J].食品科技,2000,(6):61-64.
[63] 张 燕 平 . 天 然 蜂 胶 对 自 由 基 的 清 除 作 用 [J]. 食 品 与 发 酵 工业,2002,28(1):48-52.
[64] 李蜀眉 ,宋刚 ,田维平 ,塔娜 ,傅忠实 .桔皮中黄酮化合物清除自由基的研究[J].内蒙古农业大学学报,2004,25(3):89-91.
[65] 武文洁,吕树祥,宋诗莹.芝麻提取物抗氧化性能研究[J].中国食品添加剂,2004,(1):70-72,66.
[66] 仇洁,周永标,谭玉兰,叶红梅.落葵薯提取物清除氧自由基及抗脂质过氧化作用[J].中药材,2004,27(8):608-609.
[67] 钟海雁 ,李忠海 ,魏元青 .林檎叶提取物抗氧化及·OH自由基清除作用的研究[J].北华大学学报(自然科学版),2001,2(6):522-525.
[68] 杨广花.两种天然抗氧化剂清除自由基作用的研究[J].江苏工业学院学报,2004,16(3):28-30.
[69] Venant Nihorimbere,钱和,汪何雅,陈军杰,梁清蓉.DPPH自由基比色 法 测 定 番 石 榴 叶 提 取 物 抗 氧 化 活 性 的 研 究 [J]. 食 品 科学,2004,25(7):36-42.
[70] 田云 ,卢向阳 ,易克 ,何小解 ,方俊 .天然抗氧化剂清除氧自由基的能力[J].湖南农业大学学报(自然科学版),2004,30(3):209-211.
[71] 肖丽霞,张建良.槐花抗氧化剂的提取及其抗氧化性的测定[J].江苏农学院学报,1996,17(2):71-73.
[72] 郭亚力 ,李聪 ,欧灵澄 ,王文久 ,黎勇坤 .槐米中天然抗氧化剂的提取及其抗自由基性能研究[J].食品科学,2004,25(7):154-157.
[73] 冯兰萍 ,刘在群 ,周波 ,杨立 ,刘中立 .绿茶多酚对自由基诱导的红细 胞 氧 化 性 溶 血 的 抑 制 作 用 [J]. 科 学 通报,2000,45(12):1271-1275.
[74] 张健 ,秦静芬 ,曹恩华 ,张仲伦 ,郑雁珍 .DNA损伤的化学发光法测定 和 茶 多 酚 对 它 的 保 护 作 用 [J]. 生 物 物 理 学报,1996,12(4):691-695.
[75] 方若莹,俞越汉,沈公羽.茶多酚对沙土鼠脑缺血后再灌注氧化损伤的保护作用[J].生物物理学报,1997,13(2):309-314.
[76] 叶汉侠,王甫才.18种中草药抗氧化活性的比较研究[J].浙江万里学院学报,2004,17(5):111-113.
[77] 刘玉鹏 ,刘梅 ,刘俊英 ,翁新楚 .30种中草药的抗氧化活性研究 [J].烟台大学学报(自然科学与工程版),2000,13(1):70-73.
[78] Cai Yizhong, Luo Qiong, Sun Mei and Corke Harold. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer [J]. Life Sciences, 2004, 74(17):2157-2184.
[79] 何文珊,李炎,李琳,郭祀远.几种香辛料对油脂抗氧化效果的评价[J].中国调味品,1998, (7): 12-15.
[80] Tsai Tzung-Hsun, Tsai Po-Jung and Ho Su-Chen. Antioxidant and anti-inflammatory activities of several commonly used spices [J]. Journal of Food Science, 2005, 70:93-97.
[81] Prasad K, Laxdal VA, Yu M and Raney BL. Evaluation of hydroxyl radical-scavenging property of garlic [J].Molecular and Cellar Biochemistry, 1996, 154(1):55-63.
[82] 黄水清,徐志伟.当归补血汤及当归黄芪抗氧化和抑制自由基产生的研究进展[J].中华实用中西医杂志,2004,4(17)(9):1274-1276.
[83] 蔡溱,胡晋红,全山丛,陈士明.芪灵胶囊清除超氧阴离子自由基的作用[J].中国医院药学杂志,2004,24(7):401-402.
[84] 孙冀平,沈蓓英.蛋白肽饮料的醒酒机理和制备工艺的探讨[J].食品与发酵工业,1999,25(4):64-66.
[85] Lieber CS and Decarli LM. Hepatic microsomal ethanol-oxidizing system In vitro characteristics and adaptive properties in vivo [J]. Journal of Biological Chemistry, 1970, 245(10):2505-2512.
[86] Handler JA and Thurman RG. Catalase-dependent ethanol oxidation in perfused rat liver. Requirement forfatty-acid-stimulated H202 production by peroxisomes [J]. European Journal of Biochemistry, 1988, 176:477-484.
[87] 刘 根 生 . 不 同 人 种 酒 精 代 谢 机 理 的 差 异 [J]. 生 物 学 教学,1997,(11):43,2.
[88] 蒋业贵,李兆申.酒精性肝病发病机制的研究进展[J].胃肠病学和肝病学杂志,2003,12(5):484-486.
[89] Babu PP, Kumari LR and Vemuri MC. Differential changes in cell morphology, macromolecular composition and membrane protein profiles of neurons and astrocytes in chronic ethanol treated rats [J]. Molecular and Cellular Biochemistry, 1994, 130(1):29-40.
[90] 厉 有 名 . 酒 精 性 肝 病 的 发 病 机 制 [J]. 中 华 肝 脏 病 杂志,2003,11(11):690-691.
[91] Valenzuela A, Lagos C, Schmidt K and Videla LA. Silymarin protection against hepatic lipid peroxidation induced by acute ethanol intoxication in the rat [J]. Biochemical Pharmacology, 1985, 34(12):2209-2212.
[92] 赵善民 ,黄丽娟 ,黄俊杰 ,何显教 ,晋玲 .乙醇对兔心脏活动与氧自由基的影响[J].心脏杂志,2003,15(1):7-8,11.
[93] 高小玲,尹少甫,张本忠.乙醇、乙醛对红细胞膜ATP酶活性影响的体外实验研究[J].中国工业医学杂志,2004,17(4):233-235.
[94] Situnayake RD, Crump BJ, Thurmham DI, Davies JA, Gearty J and Davis M. Lipid peroxidation and hepatic antioxidants in alcoholic liver disease [J]. Gut, 1990, 31:1311-1317.
[95] H??g J-O, Hedberg JJ, Str?mberg P and Svensson S. Mammalian alcohol dehydrogenase-functional and structural implications [J]. Journal of Biomedical Science, 2001, 8(1):71-76.
[96] Tuma DJ and Sorrell M. Alcohol and Alcoholic liver disease [J]. Seminars in Liver Disease, 2004, 24(03):215-216.
[97] Tuma DJ and Casey CA. Dangerous byproducts of alcoholbreakdown-focus on adducts [J]. Alcohol Research & Health, 2003, 27(4):285-290.
[98] Duarte R, Escario JJ. Alcohol abuse and truancy among Spanish adolescents: Acount-data approach [J]. Economics of Education Review, 2006, 25(2):179-187.
[99] Chatterji P, Dave D, Kaestner R, Markowitz S. Alcohol abuse and suicide attempts among youth [J]. Economics & Human Biology, 2004, 2(2):159-180.
[100] Rehm J, Room R, Brink WV, Jacobi F. Alcohol use disorders in EU countries and Norway: An overview of the epidemiology [J]. European Neuropsychopharmacology. 2005, 15(4):377-388.
[101] Zhang Xiaochun, Li Shi-yan, Brown RA, Ren Jun. Ethanol and acetaldehyde in alcoholic cardiomyopathy: from bad to ugly en route to oxidative stress [J]. Alcohol, 2004, 32(3):175-186.
[102] Pettinatia HM and Rabinowitz AR. Recent advances in the treatment of alcoholism [J]. Clinical Neuroscience Research, 2005, 5:151-159.
[103] 张若明 ,李经才 .解酒天然药物研究进展 [J].沈阳药科大学学报,2001,18(2):138-142.
[104] Lipsky JJ, Shen ML and Naylor S. In vivo inhibition of aldehyde dehydrogenase by disulfiram [J]. Chemico-Biological Interactions, 2001, 130-132:93-102.
[105] Thomas CP, Wallack SS, Lee S, McCarty D and Swift R. Research to practice: adoption of naltrexone in alcoholism treatment [J]. Journal of Substance Abuse Treatment. 2003, 24(1):1–11.
[106] Davidson D, Saha C, Scifres S, Fyffeb J, Connora S and Selzer C. Naltrexone and brief counseling to reduce heavy drinking in hazardous drinkers [J]. Addictive Behaviors, 2004, 29:1253–1258.
[107] Boothby LA, Pharm D, Doering PL. Acamprosate for the Treatment of Alcohol Dependence [J]. Clinical Therapeutics, 2005, 27(6):695-714.
[108] Yokozawa T, Dong E, Nakagawa T, Kashiwagi H, Nakagawa H, Takeuchi S and Chung HY. In Vitro and in vivo studies on the radical-scavenging activity of tea [J]. Journal of Agricultural and Food Chemistry, 1998, 46:2143-2150.
[109] Bozkurt H. Utilization of natural antioxidants: Green tea extract and Thymbra spicata oil in Turkish dry-fermented sausage [J]. Meat Science, 2006, 73: 442–450.
[110] 毛 连 根 , 俞 洁 行 . 茶 叶 提 取 物 对 小 鼠 的 醒 酒 作 用 [J]. 茶 叶 科学,2000,20(2):155-157.
[111] 汤银红 ,丁斌如 .枳木具 子水提物对乙醇脱氢酶活性的影响 [J].中药药理与临床,2004,20(2):24.
[112] 任发政,罗云波,蒋菁莉.枳木具 子影响乙醇代谢机理研究[J].食品科学,2002,23(12):58-60.
[113] 伍嘉宁,潘锄云,周华楚,俞国荣 ,张佳英.葛花解醒汤抗乙醇中毒的研究[J].江西中医学院学报,1994,6(4):24-25.
[114] 陈正爱 ,于海玲 ,李美子 .解酒乐的解酒作用实验研究 [J].延边大学医学学报,2005,28(4):259-260.
[115] 汤 卫 东 , 朱 海 涛 . 醒 酒 保 健 口 服 液 的 研 制 [J]. 食 品 科技,2004,(6):75-76,79.
[116] Young JL, Caarol BP, Eugene JP. Effect of ginseng on plasma level of ethanol in the rat [J]. Planta Medica, 1993, 59:17-19.
[117] Kim YK, Guo Q, Packer L. Free radical scavenging activity of red ginseng aqueous extracts [J]. Toxicology, 2002, 172: 149–156.
[118] 李敬常,周伟贞,叶丽明,周保珍 ,解长跃.三七水提物对动物乙醇损伤的保护作用[J].中成药,1992,14(6):32-33.
[119] 白雪松 ,韩春姬 ,李克 .复方轮叶党参的醒酒作用实验研究 [J].延边大学医学学报,2004,27(3):166-169.
[120] 成霈 ,顾慧莹 ,宋敏 .赶黄草对预防醉酒及解酒效能研究 [J].医药世界,2006,5:142-144.
[121] 吴淑清 ,刘杰 ,牟莉 ,黄玉珍 .沙棘米醋对酒麻过程的影响研究[J].吉林粮食高等专科学校学报,2005,20(4):5-9.
[122] Masayuki Y, Emiko H, Hisashi M, Elatosides A and B.potent inhibitors of ethanol absorption in rats from A ralia elata Seem: the structure-activity relationships of oleanolic acid oligoglycosides [J]. Chem Pharm Bull, 1993, 41(11):2069-2071.
[123] Chung JH, Cheong JC and Park KS. Effect of aloin, a quinone derivative of A loe, on ethanol metabolism in the rat [J]. Medical Science Research, 1996, 24(6):421-423.
[124] Kiyoshi S, Toshiko Y and Yamane S. Effect of water extracts of crude drugs in decreasing blood ethanol concentrations in rats [J].Chem Pharm Bull, 1987, 35(11):4597-4604.
[125] Kiyoshi S, Yukiko S and Chie I. Effect of water extracts of A loe and some herbs in decreasing blood ethanol concentration in rats II [J].Chem Pharm Bull, 1989, 37(1):155-159.
[126] 王平.拐枣果浸渍液对机体乙醇代谢的影响[J].中南林学院学报,1997,17(3):65-67.
[127] 谭海荣 ,吴谦 ,潘竞锵 ,肖柳英 ,韩超 ,林洁茹 ,郑琳颖 ,李博萍 .水飞蓟宾对抗大鼠酒精性脂肪肝作用及机制[J].中国临床药理学与治疗学,2004,9(7):803-806.
[128] 新甫勇次郎,山崎津,中岛嘉次郎.葛花对乙醇诱发的代谢异常及 实 验 性 肝 损 伤 的 影 响 [J]. 国 外 医 药 : 植 物 药 分册,1991,6(5):226-227.
[129] 王庆端,江金花,孙文欣,夏薇.葛根总黄酮的抗啤酒中枢抑制作用[J].河南医科大学学报,1998,33(3):117-119.
[130] 张恩户,赵勤,梁作峰,杨文平.葛根总黄酮解酒作用的实验观察[J].陕西中医学院学报,1995,18(3):38-39.
[131] 苏德奇,马龙,周晓辉,杨浩峰,刘涛,田丽婷.醒酒保肝胶囊对急性 酒 精 性 肝 损 伤 的 影 响 [J]. 新 疆 医 科 大 学 学报,2005,28(5):396-398.
[132] 邓源 ,班春林 ,袁伯俊 .醒酒颗粒解酒作用机制的探讨 [J].解放军药学学报,2006,22(5):350-352.
[133] 宫德正,耿成燕,刘军 ,姜丽萍,仲来福.网状五层龙热水提取物醒酒作用的实验研究[J],毒理学杂志,2005,19(3):217-218.
[134] 黄永平,周世文,徐颖,汤建林,王莲,张津源,杨雪.乙酰半胱氨酸对小鼠酒精中毒的影响[J],中国药房,2004,15(7):403-404.
[135] 袁秉祥,孙晓东,罗笑宇,于晓江.永生宜康醒酒和减轻酒精致动物肝损伤药效学试验[J].酿酒科技,2005,(4):90-92.
[136] 傅兰,傅伟,沈伟,赵玉珍.白酒加入宜康后减轻对人体损伤的实验[J].酿酒,2004,31(1):85-86.
[137] 邓源,袁伯俊,张淑英.解酒灵对乙醇致鼠肝急性损伤的保护作用及机制的探讨[J].中国新药杂志,2000,9(6):388-391.
[138] 季光,曹承楼,张玮,邢练军,王奕,王育群.清肝活血方对酒精性肝 病 大 鼠 ADH 及 CYPⅡE1 的 影 响 [J]. 中 西 医 结 合 肝 病 杂志,2002,12(4):215-217.
[139] 周毅刚,徐绍锐,舒坤贤,文继舫 ,李昂.醒酒灵冲剂对机体内乙醇代谢的影响[J].湖南医学,2001,18(3):212,216.
[140] 周红宇,胡国新,胡爱萍,丁细桃 ,郑荣远.醒脑静注射液对乙醇所 致 记 忆 功 能 障 碍 的 影 响 [J]. 中 国 临 床 药 理 学 与 治 疗学,2004,9(1):110-112.
[141] 芦洁,李文哲,倪亚明.中药水提取物对乙醇脱氢酶活性影响的初步研究[J].同济大学学报(医学版),2002,23(1):23-24,30.
[142] Ganapathy V, Brandsch M and Leibach FH.Intestinal transport of amino acids and peptides [M]. In: Johnson LR ed, Physiology of the gastrointestinal tract, New York: Raven Press, 1994, 52:1773.
[143] Ehud Z, Moise B. Intestinal Absorption of peptides throught the enterocytes [J]. Microscopy Research and Technique, 2000, 49(4):346-352.
[144] Fei Y-J, Kanai Y, Nussberger S, Ganapathy V, Leibach FH, Romero MF, Singh SK, Boron WF and Hediger MA.Expression cloning of a mammaliam proton-coupled oligopeptide transporter [J]. Nature, 1994, 368:563-566.
[145] 陈历俊 ,程涛 ,骆承序 .肽基蛋白水解物及其生物活性 [J].食品工 业 科 技 ,1999, 增 刊 — 北 京 食 品 学 会 成 立 二 十 周 年 论 文集:178-182.
[146] 曾晓波,王海英,林永成.食物中的生物活性肽:生物活性及研究进展[J].食品工业科技,2004,25(4):151-155.
[147] Nagaoka S, Awano T, Nagata N, Masaoka M, Hori G and Hashimoto K. Serum cholesterol reduction and cholesterol absorption inhibition in Caco-2 cells by a soyprotein peptic hydrolyzate [J]. Bioscience, Biotechnology and Biochemistry, 1997, 61:354-356.
[148] Hughes J. Isolation of an endogenous compound from the brain with the pharmacological properties similar to morphine [J]. Brain Research, 1975, 88(2):295-308.
[149] Gill I, López-Fandi?o R, Jorba X and Vulfson EN. Biologically active peptides and enzymatic approaches to their production [J]. Enzyme and Microbial Technology, 1996, 18(3):162-183.
[150] Ferreira IMPLVO, Pinho O, Mota MV, Tavares P, Pereira A, Gon?alves MP, Torres D, Rocha C and Teixeira JA. Preparation of ingredients containing an ACE-inhibitory peptide by tryptic hydrolysis of whey protein concentrates [J].International Dairy Journal, 2007, 17(5):481-487.
[151] 杨浩峰 ,马龙 .乳源性生物活性肽研究进展 [J].乳业科学与技术,2006,(2):53-55.
[152] 王专 ,易华西 ,吴永英 .乳酪蛋白源生物活性肽的研究进展 [J].生物信息学,2006,4(2):95-97.
[153] Je J-Y, Park P-J, Kim S-K. Antioxidant activity of a peptide isolated from Alaska Pollack (Theragra chalcogramma) frame protein hydrolysate [J]. Food Research International, 2005, 38:45-50.
[154] Rajapakse N, Mendis E, Jung W-K, Je JY and Kim S-K. Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties [J]. Food Research International, 2005, 38:175-182.
[155] Yoshida T, Muto Y, Moriwaki H and Yamato M. Effect of long-term oral supplementation with branched-chain amino acid granules on the prognosis of liver cirrhosis [J]. Gastroenterologia Japonica, 1989, 24(6):692-698.
[156] Ravallec-Plé R, Charlot C, Pires C, Braga V, Batista I, Wormhoudt AV, Gal YL and Fouchereau-Péron M.The presence of bioactive peptides in hydrolysates prepared from processing waste of sadine (Sardina pichardus) [J]. Journal of the Science of Food and Agriculture, 2001, 81(11):1120- 1125.
[157] Jeon Y-J, Byun H-G and Kim S-K. Improvement of functional properties of codframe protein hydrolysates using ultrafiltration membranes [J]. Process Biochemistry, 1999, 35(5):471-478.
[158] Wu H C, Chen H-M and Shiau C-Y. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus) [J]. Food Research International, 2003, 36:949-957.
[159] Korhonen H, Pihlanto-Lepp?la A, Rantam?ki P and Tupasela T. Impact of processing on bioactive proteins and peptides [J]. Trendsin Food Science and Technology, 1998, 9(8-9):307- 319.
[160] 焦迎春,郑晓冬.活性肽在食品中的应用研究[J].粮油加工与食品机械,2002,(8):35-37.
[161] 张晓梅,钟芳,麻建国.大豆降胆固醇活性肽的初步分离纯化[J].食品与机械,2006,22(2):33-37.
[162] 陈 成 . 大 豆 蛋 白 活 性 肽 保 健 功 能 性 的 研 究 [J]. 大 豆 通报,2005,(2):22-24.
[163] 赵芳芳,张日俊.大豆肽研究进展[J].中国饲料,2004,(1):22-23.
[164] 荣建华 ,李小定 ,谢笔钧 .大豆肽体外抗氧化效果的研究 [J].食品科学,2002,23(11):118-120.
[165] 翟瑞文 ,李雁群 ,余世望 .用玉米渣生产玉米蛋白肽饮料 [J].食品科学,1997,18(9):31-33.
[166] Alexandra Alves-Rodrigues and Andrew Shao. The science behind lutein [J]. Toxicology Letters, 2004, 150(1):57-83.
[167] 周大寨 ,黄国清 ,唐巧玉 .用 HPLC测定酶解玉米蛋白氨基酸的组成[J].食品科学,2005,26(2):179-181.
[168] 王梅,谷文英.酶解玉米黄粉蛋白制备可溶性肽[J].粮油食品科技,1999,17(1):1-5.
[169] 金英姿,庞彩霞.碱性蛋白酶水解玉米蛋白工艺条件的研究[J].食品研究与开发,2006,27(3):44-45.
[170] 崔 东 善 . 蛋 白 酶 及 其 在 食 品 中 的 应 用 [J]. 杭 州 食 品 科技,1997,44(1):15-19,22.
[171] 张晓峰 ,陈庆森 ,庞广昌 .酶解 100kg玉米蛋白粉制备玉米功能短肽的中试研究[J].食品工业科技,2006,(7):58-59.
[172] Peter RS and Nigel GH. Cereal seed storage proteins: structures, properties and role in grain utilization [J]. Journal of Experimental Botany, 2002, 53:947-958.
[173] 鲁晓翔,陈新华,唐津忠.酶法改性玉米蛋白功能特性的研究[J].食品科学,2000,21(12):13-15.
[174] 佐 野 良 史 . コ ㄧ ン ぺ チ ド の 食欠 料 へ の 利 用 [J]. 食 品 と 科学,1993,2:102-106.
[175] 陈 文 , 陈 代 文 . 玉 米 蛋 白 粉 的 饲 用 价 值 及 鉴 别 [J]. 饲 料 博览,2003,(9):29-30.
[176] 张艳铭 ,甄二英 ,宋智娟 .玉米蛋白粉的营养价值及其应用 [J].当代畜牧,2006,(1):22-24.
[177] 郭亮,安秀芳,李淑英,郭帅.玉米蛋白粉营养价值评定及其评定技术的研究[J].中国畜牧兽医,2004,31(3):16-18.
[178] 王 玉 军 , 单 立 志 . 玉 米 蛋 白 在 食 品 中 的 应 用 [J]. 黑 龙 江 粮食,2003,(3):35-36.
[179] 李大婧,杨同舟,季亮,王玉军.玉米蛋白粉在食品中的应用研究[J].粮油食品科技,2001,9(1):12-14.
[180] 江洪波,左艳文.玉米蛋白发泡粉的生产工艺[J].长江大学学报(自然版),2006,3(2):188-190.
[181] 江洪波,左艳文.玉米蛋白发泡粉生产工艺的研究[J].安徽农学通报,2006,12(6):210-211.
[182] 昌 友权 ,郑鸿雁 ,张建峰 ,陈立阁 ,程宏 .精制玉米蛋白粉包埋DHA的研究[J].吉林农业大学学报,2001,23(1):107-110.
[183] 李晶,张丽萍.精制玉米蛋白粉在DHA中的应用研究[J].食品工业科技,2004,25(8):67-69.
[184] 王学智,任桂久,吴之传,谢同云,耿传,丁胜利.研制玉米蛋白基生 物 降 解 性 塑 料 的 初 步 结 果 [J]. 安 徽 机 电 学 院 学报,2000,15(1):10-14.
[185] 王学智.玉米蛋白基生物降解性塑料的初步研究[J].实验与创新思维,2004,(12):17-19.
[186] 胡 学 烟 , 孙 冀 平 . 玉 米 蛋 白 粉 开 发 与 利 用 [J]. 粮 食 与 油脂,2001,(11):32-33.
[187] 孙震,苏宇杰,姚惠源.玉米蛋白粉中黄体素、玉米黄素对乳腺癌细胞增殖的影响[J].食品与机械,2005,21(2):6-11.
[188] 王薇,韩鲁佳.玉米蛋白粉中叶黄素提取及皂化工艺的研究[J].中国农业大学学报,2006,11(4):125-130.
[189] 李 勇 , 赵 杰 文 . 玉 米 黄 色 素 的 提 取 纯 化 研 究 [J]. 中 国 粮 油 学报,2006,21(3):74-77.
[190] 孙震,李博,姚惠源.玉米蛋白粉中叶黄素和玉米黄素对口腔癌细胞DNA的损伤作用[J].食品科技,2006,(4):131-134.
[191] 孙震,奚海燕,陈正行,姚惠源.玉米蛋白粉中叶黄素和玉米黄素对 小 鼠 巨 噬 细 胞 免 疫 调 节 活 性 的 研 究 [J]. 食 品 科学,2006,27(11):505-509.
[192] 孙震,姚惠源.玉米蛋白粉中玉米黄素对人口腔鳞癌细胞株KB增殖的抑制[J].食品与生物技术学报,2005,24(6):34-37.
[193] 程谦伟,郭兴凤,熊拯.玉米的综合开发—醇溶蛋白的提取[J].粮食科技与经济,2006,(6):43-45.
[194] 刘志国 ,胡丽丽 ,史鑫 .玉米醇溶蛋白的制备 [J].武汉食品工业学院学报,1997,(3):6-9.
[195] 姚晓敏,孙向军,卢杰.可食性玉米醇溶蛋白成膜工艺的研究[J].食品工业科技,2002,23(1):20-23.
[196] 张钟,徐丽红、桑红庆.玉米营养保健口香糖的研制[J].食品工业,2004,(6):12-13.
[197] 李文静,田崇明,张永申,胡卫国.玉米醇溶蛋白在糖果中的应用研究[J].食品工业科技,2002,23(5):31-32.
[198] 田崇明,李文静,胡卫国.玉米醇溶蛋白在香蕉保鲜中的应用研究[J].食品科技,2002,(3):66,69.
[199] 庞 彩 霞 , 金 英 姿 . 玉 米 醇 溶 蛋 白 的 应 用 [J]. 内 蒙 古 农 业 科技,2005,(4):40-41.
[200] Olsman H. Hydrolyzed and autolyzed vegetable proteins as functional food ingredients [J]. Journal of the American Oil Chemists Society, 1979, 56:375-376.
[201] 郭瑶,曾名勇,崔文萱.水产胶原蛋白及胶原多肽的研究进展[J].水产科学,2006,25(2):101-104.
[202] 曾名勇,崔海英,李八方.海洋生物活性肽及其生物活性研究进展[J].中国海洋药物杂志,2005,24(1):46-51.
[203] 刘云国,李八方,汪东风,赵雪,林琳,冯大伟,毕琳.海洋生物活性肽研究进展[J].中国海洋药物杂志,2005,24(3):52-57.
[204] 翟瑞文,李雁群,陈子林,余世望.玉米肽的制备特性与应用[J].食品工业,1998,3:11-14.
[205] 叶启腾 ,陈强 .木瓜蛋白酶的应用 [J].广西热作科技 ,1999 (4): 34-35.
[206] 王遂,尤旭.微生物蛋白酶对玉米皮蛋白水解作用的研究[J].食品工业科技,2000,21(4):22-24.
[207] 李艳丽,陈光,丛建民,孙扬,刘艳秋,吴歌.碱性蛋白酶水解法制备玉米肽的研究[J].吉林农业大学学报,2004,26(2):142-144,149.
[208] Xu L, Wu X-x, Fei X-f, Zhang L-q and Zhang X-z. A study on bioactivity of corn peptides with low molecular weight Ⅰ: Effect of intake of them on alcohol metabolism in rats [J]. Chemical Research in Chinese Universities, 2003, 19(2):180-182.
[209] Xu L, Zhang L-q, Wu X-x, Wang N and Zhang X-z. A study of bioactivity of corn peptides with low molecular weight Ⅱ: Effect on plasma free amino acid concentrations in rats [J]. Chemical Research in Chinese Universities, 2003, 19(3):314-316.
[210] 刘志国,陈江源,王亚林,刘生毅.玉米醇溶蛋白酶解制备ACE抑制肽的研究[J].食品研究与开发,2006,27(2):138-141.
[211] 崔凌飞 ,王遂 .Alcalase蛋白酶水解玉米皮蛋白的研究 [J].哈尔滨师范大学自然科学学报,2002,18(1):62-66.
[212] 韩继福,任建波.Alcalase酶水解玉米蛋白粉制备可溶性肽最佳条件的研究[J].吉林农业科学,2003,28(3):46-49.
[213] 陈新,陈庆森,庞广昌.不同变性方式对Alcalase AF214L酶解玉米蛋白水解度的影响[J].食品与发酵工业,2005,31(2):42-44.
[214] 邓涛,刘利锋.玉米蛋白水解液在温度敏感型菌种中的尝试[J].发酵科技通讯,2006,35(2):29-30.
[215] 杨剑 ,卢昌义 ,于兴娜 .4种玉米水解肽的除草活性及与相对分子质量的关系[J].生态与农村环境学报,2006,22(3):33-36.
[216] 郑喜群,李里特,吴红艳,刘晓兰.利用米曲霉羧肽酶由玉米蛋白粉制备高F值寡肽[J].食品科学,2004,25(增刊),43-46.
[217] 李 峰 , 徐 军 庆 . 高 F 值 寡 肽 的 研 究 与 开 发 [J]. 食 品 与 药品,2005,7(1A):43-44.
[218] 谷文英,过世东,沈培英,王梅.酶解玉米蛋白粉制备高F值寡肽[J].精细与专用化学品,2001,(21):22-23.
[219] 王晓杰,郑喜群,刘晓兰,林杰,王丽丽,李里特.利用纳豆杆菌发酵玉米蛋白粉新工艺研究[J].食品研究与开发,2006,27(5):42-45.
[220] 何慧,谢笔钧,杨卓,唐谊练.大豆蛋白和玉米蛋白酶解肽及其活性研究[J].粮油食品科技,2002,10(1):14-16.
[221] Hardwick JE and Glatz CE. Enzymatic Hydrolysis of Corn Gluten Meal [J]. Journal of Agricultural and Food Chemistry, 1989, 37:1188-1192.
[222] 李升福,闻海波.玉米蛋白水解物的功能性—抗疲劳的研究[J].食品科学,2006,27(08):245-247.
[223] 昌 友 权 . 玉 米 肽 抗 疲 劳 作 用 的 实 验 研 究 [J]. 食 品 科学,2004,25(9):173-178.
[224] 李 晶 . 玉 米 肽 抗 疲 劳 作 用 的 实 验 研 究 [J]. 食 品 与 机械,2004,20(1):11-12,27.
[225] Mills ENC, Alcocer MJC and Morgan MRA. Biochemical interactions of food-derived peptides [J]. Trends in Food Science and Technology, 1992, 3:64-68.
[226] Liu DL-Y, Christians NE and Garbutt JT. Herbicidal activityof hydrolyzed corn gluten meal on three grass species under controlled environments [J]. Journal of Plant Growth Regulation,1994, 13(4):221-226.
[227] Liu DL-Y and Christians NE. Isolation and identification of root-inhibiting compounds from corn gluten hydrolysate [J]. Journal of Plant Growth Regulation, 1994, 13(4):227-230.
[228] Yamaguchi M, Takada M, Nozaki O, Ito M and Furukawa Y. Preparation of corn peptides from corn gluten meal and its administration effect on alcohol metabolism in stroke-prone spone spontaneously hypertensive rats [J]. Journal of Nutritional Science and Vitaminology, 1996, 42:219-231.
[229] Yamaguchi M, Nishikiori F, Ito M and Furukawa Y. The effects of corn peptide ingestion on facilitating alcohol metabolism in healthy men [J]. Bioscience Biotechnology and Biochemistry, 1997, 61(9):1474-1481.
[230] 徐力,刘立,李相鲁,乔岩梅,吴晓霞,张学忠.玉米功能短肽的制备 及 其 类 超 氧 化 物 歧 化 酶 活 性 研 究 [J]. 中 国 生 化 药 物 杂志,2002,23(2):78-80.
[231] 徐力 ,李相鲁 ,黄宜兵 ,吴晓霞 ,候瑞珍 ,王华 ,王娜 ,张学忠 .小分子玉米肽Leu-Asp-Tyr-Glu保护线粒体抗氧化损伤的研究[J].高等学校化学学报,2004,25(6):1073-1075.
[232] 孙红,于得伟,崔志勇,杨明.玉米肽对动物实验性肝损伤的保护作用[J].中药药理与临床,2002,18(3):10-111.
[233] Adamson N J and Reynolds EC. Characterization of casein phosphopeptides prepared using alcalase: Determination of enzyme specificity [J]. Enzyme and Microbial Technology, 1996, 19:202-207.
[234] 黄伟坤.食品检验与分析[M].北京:中国轻工业出版社: 1989,第1版,23-25.
[235] 王文侠.超临界二氧化碳萃取米糠油的生产工艺研究[J].工艺技术,2005,26(8):107-112.
[236] 王红菊,郭维静,郭洪臣,班玉凤,陈黎行,王祥生.一种提高玉米蛋白水解度和降解率的方法[J].食品科技,2005,(6):92-95.
[237] 郭维静,权文吉,陈黎行,郭洪臣 ,王祥生.玉米蛋白粉的剪切乳化 处 理 及 酸 性 蛋 白 酶 的 水 解 研 究 [J]. 食 品 工 业 科技,2006,27(10):103-105.
[238] Kong B and Xiong YL. Antioxidant activity of zein hydrolysates in a liposome system and the possible mode of action [J]. Journal of Agricultural and Food Chemistry, 2006, 54:6059–6068.
[239] 李自升 ,蔡木易 ,易维学 .玉米蛋白粉的酶解工艺初探 [J].食品与发酵工业,2006,32(4):67-69.
[240] 隋玉杰,何慧,王进,贺菊萍,胡筱波.中性蛋白酶及碱性蛋白酶制 备 玉 米 粗 肽 的 醒 酒 活 性 比 较 [J]. 中 国 粮 油 学报,2006,21(3):102-106.
[241] 郑冬梅 ,李升福 ,孔保华 .玉米蛋白水解条件的优化研究 [J].食品科学.2002,23(8):52-56.
[242] 许宗运,马少宾,张秀萍,赵卫国,刘利林,吕银龙.DPPH法评价37种植物抗氧化活性[J].塔里木农恳大学学报,2004,16(2):1-4.
[243] Milardovié S, Ivekovic D and Grabaric BS.A novel amperometric method for antioxidant activity determination using DPPH free radical [J]. Bioelectrochemistry, 2006, 68:175–180.
[244] Marklund S and Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase [J]. European Journal of Biochemistry, 1974, 47:469-474.
[245] 吕永为,郭详群.荧光分光光度法测定两种中药对羟自由基的清除作用[J].厦门大学学报(自然科学版),2004,43(2):208-212.
[246] 杨 祥 宇 , 宋 健 , 冯 荣 秀 . 荧 光 标 记 染 料 [J]. 化 学 通报,2003,66(9):615～621.
[247] Yamaguchi M, Nishikiori F, Nozaki O, Ito M and Furukawa Y. Effcet of long-team ‘corn peptide’ ingestion on alcohol metablism in stroke-prone spontaneously hypertensiverats with alcohol loading [J]. Journal of Nutritional Science and Vitaminology, 1996, 42:567-580.
[248] Yamaguchi M, Nishikiori F, Ito M and Furukawa Y. Effcet of corn peptide on alcohol metabolism and plasma free amino acid concentrations in healthy men [J]. European Journal of Clinical Nutrition, 1996, 50:682-688.
[249] [德]B.施特尔马赫著,钱嘉渊译.酶的测定方法[M].中国轻工业出版社:1992,第1版,17-19.
[250] 陈灏珠.实用内科学(上册)[M].北京:人民卫生出版社,2003,第11版:789-790.
[251] Young IS and McEneny J. Lipoprotein oxidation and atherosclerosis [J]. Biochemical Society Transactions, 2001, 29(Part 2):358-362.
[252] 方 允 中 , 杨 胜 , 伍 国 耀 . 自 由 基 稳 衡 性 动 态 [J]. 生 理 科 学 进展,2004,35(3):199-204.
[253] Gebicki JM. Comparison of the capacities of the perhydroxyl and the superxide radicals to initiate chain oxidation of linolieic acid [J]. American Chemical Society, 1981, 103(23):7020-7022.
[254] Mak IT, Kramer JK and Weglicki WB. Potention of free radical-induced lipid peroxidative injury to sarcolemmal membranes by lipid amphiphiles [J]. Journal of Biological Chemistry, 1986, 261:1153-1157.
[255] Chung AS and Maines MD. Effect of selenium on glutathione metabolism: Induction of γ-glutamylcysteine synthetase and glutathione reductase in the rat liver [J]. Biochemical Pharmacology, 1981, 30(23):3217-3223.
[256] Hodgson EK and Fridovich L. The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide. Inactivation of the enzyme [J]. Biochemistry, 1975, 14(24):5294-5299.
[257] Zloch Z. Oxidation/antioxidation status after an acute ethanol intoxication [J]. Toxicology Letters, 1995, 78(supplement 1):87.
[258] ?ner-yidogan Y, Gürd?l F and ?ner P. The effects of acute melatonin and ethanol treatment on antioxidant enzyme activities in rat testes [J]. Pharmacological Research, 2001, 44(2):89-93.
[259] Vendemiale G, Grattagliano I, Signorile A and Altomare E. Ethanol-induced changes of intracellular thiol compartmentation and protein redox status in the rat liver: effect of tauroursodeoxycholate [J]. Journal of Hepatology, 1998, 28(1):46-53.
[260] Yamaguchi M, Takeuchi M, Ebihara K. Inhibitory effect of peptide prepared from corn gluten meal on 7,12-Dimethylbenz anthracene-induced mammary tumor progression in rats [J]. Nutrition Research, 1997, 17(7):1121-1130.
[261] 张权义,欧阳云.多样品减重法测定乳制品中脂肪含量实验分析[J].实用预防医学,2004,11(2):279-280.
[262] Jens Adler-Nissen. Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid [J]. Journal of Agricultural and Food Chemistry, 1979, 27(6):1256-1262.
[263] 张龙翔,张庭芳,李令媛主编.生化实验方法和技术[M].北京:高等教育出版社,1997,第1版,137-139.
[264] Chen S-T, Chen S-Y, Wang K-T. Kineticaly controlled peptide bond formation in anhyolrons alcohol catalyzed by the industrial protease Alcalase [J]. Journal of Organic Chemistry, 1992,57:6960-6965.
[265] Hojilla-Evangelista MP and Johnson LA. Optimizing extraction of zein and glutelin-rich fraction during sequential extraction processing of corn [J]. Cereal Chemistry, 2003, 80(4):481-484.
[266] Lawton JW. Zein:A history of processing and use [J].Cereal Chemistry, 2002, 79(1):1-18.
[267] 黄国平,温其标.超声波法提取玉米醇溶蛋白的研究[J].食品与发酵工业,2002,28(10):1-5.
[268] 张秋荣,吴春丽,单丽红,刘艳凯 ,李良辰.玉米蛋白粉中醇溶蛋白 超 声 萃 取 工 艺 改 进 [J]. 郑 州 大 学 学 报 ( 医 学版),2005,40(5):913-915.
[269] 张钟,齐爱云.玉米醇溶蛋白提取工艺及功能性质研究[J].粮食与饲料工业,2004,(9):21-23.
[270] Mannheim A and Cheryan M. Water-soluble zein by enzymatic modification in organic solvents [J].Cereal Chemistry, 1993, 70(2):115-121.
[271] 徐力,李相鲁,吴晓霞,王华,候瑞珍,黄宜兵,张学忠.一种新的玉米 抗 氧 化 肽 的 制 备 与 结 构 表 征 [J]. 高 等 学 校 化 学 学报,2004,25(3):466-469.
[272] Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. Protein measurement with the Folin phenol reagent [J]. The Journal of Biological Chemistry, 1951, 193:265-275.
[273] Summer JP, Sisler EB. A simple method for blood sugar [J]. Archives of Biochemistry and Biophysics, 1944, 4:333.
[274] Miller GL. Use of dinitrosalicylie acid reagent for determination of reducing sugar [J]. Analytical Chemistry, 1959, 31(3):426-428.
[275] 王镜岩、朱圣庚、徐长法.生物化学(上册)[M],高等教育出版社:2003,第3版,103.
[276] Ozcelik B, Lee JH and Min DB. Effects of light, oxygen and pH on the absorbance of 2,2-diphenyl-1-picrylhydrazyl [J]. Journal of Food Science, 2003, 68(2):487–490.
[277] Yamazaki E, Inagaki M, Kurita O and Inoue T. Antioxidant activity of Japanese pepper (Zanthosylum piperitum DC.) fruit [J]. Food Chemistry, 2007, 100(4):171-177.
[278] Shimada K, Fujikawa K, Yahara K and Nakamurat T. Antioxidative properties of xanthan on the antioxidation of soybean oil in cyclodextrin emulsion [J]. Journalof Agricultural and Food Chemistry, 1992, 40:945-948.
[279] Cotelle N, Bernier J-L, Catteau J-P, Pommery J, Wallet J-C and Gaydou EM. Antioxidant Properties of Hydroxy-Flavones [J]. Free Radical Biology and Medicine, 1996, 20(1):35-43.
[280] Hu F, Schmidt K, Stefka S, Li Z, Udo G, Matthias H. Radical scavengers from the entomogenous deuteromycete beauveria amorpha [J]. Planta Medica, 2002, 68:64～65.
[281] 李永利,张焱.邻苯三酚自氧化法测定SOD活性[J].中国卫生检验杂志,2000,10(6):673.
[282] 张宏,谭竹钧.四种邻苯三酚自氧化法测定超氧化物歧化酶活性 方 法 的 比 较 [J]. 内 蒙 古 大 学 学 报 ( 自 然 科 学版),2002,33(6):677-681.
[283] 李建武,萧能庚,余瑞元等.生物化学实验原理和方法[M].北京大学出版社:1997,第1版,408.
[284] 翟继英.光度法测定羟自由基及蔬菜抗氧化性[J].卫生职业教育,2005,23(7):107-108.
[285] Ranathunga S, Rajapakse N and Kim S-K. Purification and characterization of antioxidative peptide derived from muscle of conger eel (Conger myriaster) [J]. European Food Research and Technology, 2006, 222(3-4):310-315.
[286] Oyaizu M. Antioxidative activities of browning products of glucosaming fractionated by organic solvent and thin-layer chrlmatography [J]. Nippon Shokuhin Kogyo Gakkaishi, 1988, 35:771-775.
[287] Haseba T, Tomita Y, Kurosu M and Ohno Y. Dose and time changes in liver alcohol dehydrogenase (ADH) activity during acute alcohol intoxication involve not only class I but also class III ADH and govern elimination rate of blood ethanol [J]. Legal Medicine, 2003, 5:202-211.
[288] Li SY, Gomelsky M, Duan J, Zhang Z, Gomelsky L, Zhang X, Epstein PN and Ren J. Overexpression of aldehyde dehydrogenase-2 (ALDH2) transgene prevents acetaldehyde-induced cell injury in human umbilical vein endothelial cells [J]. The Journal of biological chemistry, 2004, 279(12):11244-11252.
[289] Moon K-H, Kim B-J, Song BJ. Inhibition of mitochondrial aldehyde dehydrogenase by nitric oxide-mediated S-nitrosylation [J]. FEBS Letters, 2005, 579:6115–6120.
[290] Chrostek L, Szmitkowski M and Jelski W. Alcohol and aldehyde dehydrogenase activity measured with fluorogenic substrates in the liver of rats poisoned with methanol [J]. Experimental Toxicologic Pathology, 2001, 53:77-80.
[291] Mezey E, Rennie-Tankersley L and Potter JJ. Effect of leptin on liver alcohol dehydrogenase [J]. Biochemical and Biophysical Research Communications, 2005, 337:1324-1329.
[292] 邢其毅,徐瑞秋,周政,裴伟伟.基础有机化学[M].高等教育出版社: 2000,第1版,454-459.
[293] Conduah Birt JEE, Shuker DEG and Farmer PB. Stable acetaldehyde-protein adducts as biomarkers of alcohol exposure[J]. Chemical Research in Toxicology, 1998, 11:136-142.
[294] Klassen LW, Jones BL, Sorrell MF, Tuma DJ. Thiele GM. Conversion of acetaldehyde-protein Adduct epitopes from a nonreduced to a reduced phenotype by antigen processing cells [J]. Alcoholism: Clinical and Experimental Research, 1999, 23(4):657-663.
[295] Thiele GM Miller JA, Klassen LW and Tuma DJ. Long-term ethanol administration alters the degradation of acetaldehyde adducts by liver endothelial cells [J]. Hepatology, 1996, 24(3):643-648.
[296] 金宗濂,文镜,唐粉芳 ,陈文.功能食品评价原理及方法[M]. 北京大学出版社:第1版,1995,10.
[297] 司 徒 镇 强 , 吴 军 正 . 细 胞 培 养 [M]. 世 界 图 书 出 版 社 :( 修 订版)2005,251-252.
[298] 孙素兰,俞励平,文洁 ,叶青山,朱建梅.灵芝片抗疲劳作用实验研究[J].食品与药品,2006,8(6A):44-46.
[299] 刘静波,林松毅,程胜.主成分分析方法综合评价功能食品抗疲劳和耐缺氧功效特性[J].中国食品学报,2006,6(1):212-217.
[300] Park K-J and Hyun C-K. Antigenotoxic effects of the peptides derived from bovine blood plasma proteins [J]. Enzyme and Microbial Technology, 2002, 30(5):633- 638.
[301] Monti JC and Jost R. Enzymatic solubilization of heat-denatured cheese whey protein [J]. Journal of Dairy Science, 1978, 61:1233-1237.
[302] 付清泉,左斌海,李天全,万昌秀.几类生物活性肽的研究进展[J]航天医学与医学工程,2002,15(3):227-230.