益气健脾法对AIDS患者免疫重建的理论与临床研究
摘要
目的:观察益气健脾汤对AIDS患者治疗前后症状、体征、积分变化,生存质量影响。外周血T细胞活化指标CD4+T淋巴细胞、CD8+T淋巴细胞、CD4/CD8、CD4+CD45RA、HIV病毒载量及IL-2、IL-10、TNF等细胞因子在不同时间点的变化。探讨本方对免疫功能的影响,为深入研究中医药对HIV/AIDS免疫重建的影响提供临床依据。
方法:严格筛选符合实验要求的AIDS病人60例,均在已经服用HAART药物的基础上,随机分成两组,每组30例。两组分别给予抗HIV组方(AZT+3TC+NVP)+中药和抗HIV组方(AZT+3TC+NVP)。两组患者分别在治疗的第0、3、6、个月观察临床症状体征变化、进行CD4+T淋巴细胞、CD8+T淋巴细胞、CD4/CD8、CD4+CD45RA、病毒载量检测,同时在第0、6月进行安全性指标血常规、尿常规、X线、心电图、肝功能、肾功能的测定。
结果:中西医结合治疗组和对照组起始症状总积分分别为20.37±3.98和22.1±6.04(P>0.05);三个月后分别为13.30±4.12和15.70±5.07(P<0.05);6个月时分别为3.46±2.34和12.10±4.13(P<0.01),症状体征总积分明显优于HAART对照组。卡洛夫斯基积分中西医结合治疗组在疗后各月均优于对照组(P<0.05)。中西医结合治疗组和对照组CD4+T淋巴细胞计数起始为371.70±82.93和338.40±77.73(P>0.05);三个月后为414.33±81.77和360.63±77.01;六个月时476.40±87.50和408.80±78.87(P<0.05)有显著差异。CD8+T淋巴细胞计数、起始为1144.10±280.84和1154.20±350.36(P>0.05);三个月时为1309.90±307.52和1133.50±265.43六个月时为1424.50±425.10和1225.70±279.17(P<0.05)有显著差异。CD4/CD8、CD4+CD45RA组间比较无差异(P>0.05),两组病人HIV病毒载量检测治疗前后均组间组内比较均<500copies/ml。两组治疗前后组间比较安全性指标(血尿常规、X线、心电图、肝肾功能及血糖、血脂)均无统计学差异。
结论:中药益气健脾汤能够有效改善AIDS患者的临床症状体征,提高生活质量;对T淋巴细胞百分比无明显影响,但有效提升患者的CD4+T淋巴细胞计数,增强了患者的免疫功能;中西药合用未影响HAART抑制病毒复制的作用;未发现对细胞因子有显著影响。
1 Objective
Observed in patients before and after treatment of AIDS symptoms, signs, points change, quality of life impact. Indicators of T cell activation in peripheral blood CD4 + T lymphocytes, CD8 + T lymphocytes, CD4/CD8, CD4 + CD45RA, HIV viral load and IL-2, IL-10, TNF and other cytokines at different time points of change. Of the side effects on immune function, for in-depth study of Chinese medicine for HIV / AIDS impact of immune reconstitution to provide clinical basis.
2 Method
Strictly meet the test requirements of the AIDS screening 60 patients were already taking HAART drugs, based on randomly divided into two groups of 30 cases. Anti-HIV group were given two sides (AZT +3 TC + NVP) + Chinese and anti-HIV group side (AZT +3 TC + NVP). Two groups of patients were in the treatment of the first 0,3,6, months, signs of change in clinical symptoms, the CD4 + T lymphocytes, CD8 + T lymphocytes, CD4/CD8, CD4 + CD45RA, viral load testing, while the first month safety index 0,6 blood, urine, X ray, electrocardiogram, liver function, kidney function measurement.
3 Results
In the combination therapy group and the control group were initial symptom total score of 20.37±3.98 and 22.1±6.04 (P> 0.05); three months were 13.30±4.12 and 15.70±5.07 (P <0.05); 6 months was 3.46±2.34, respectively, and 12.10±4.13 (P <0.01), total score of symptoms and signs HAART was better than the control group. Carlo Minkowski integral combination therapy group were in each month after treatment than the control group (P <0.05). In the combination therapy group and the control group starting CD4 + T lymphocyte count was 371.70±82.93 and 338.40±77.73 (P> 0.05); three months after 414.33±81.77 and 360.63±77.01; six months 476.40±87.50 and 408.80±78.87 (P <0.05) were significantly different. CD8 + T lymphocyte count, starting at 1144.10±280.84 and 1154.20±350.36 (P> 0.05); three months to 1309.90±307.52 and 1133.50±265.43 1424.50±425.10 for the six months and 1225.70±279.17 (P < 0.05) were significantly different. CD4/CD8, CD4 + CD45RA no difference between the two groups (P> 0.05), HIV viral load in two groups of patients before and after treatment group were compared between the groups were <500copies/ml. Before and after treatment between the two groups safety index (urine routine, X ray, electrocardiogram, renal function and blood glucose, blood lipids) were not statistically different.
4 Conclusion
Spleen Qi decoction of Chinese medicine can improve clinical symptoms and signs of AIDS and improve the quality of life; on the percentage of T lymphocytes had no effect, but the effective increase in patients with CD4 + T lymphocyte count, and enhance the patient's immune function; Chinese and Western medicine Combined inhibition of viral replication did not affect the role of HAART; not found on the cell factor had significant effect.
方法:严格筛选符合实验要求的AIDS病人60例,均在已经服用HAART药物的基础上,随机分成两组,每组30例。两组分别给予抗HIV组方(AZT+3TC+NVP)+中药和抗HIV组方(AZT+3TC+NVP)。两组患者分别在治疗的第0、3、6、个月观察临床症状体征变化、进行CD4+T淋巴细胞、CD8+T淋巴细胞、CD4/CD8、CD4+CD45RA、病毒载量检测,同时在第0、6月进行安全性指标血常规、尿常规、X线、心电图、肝功能、肾功能的测定。
结果:中西医结合治疗组和对照组起始症状总积分分别为20.37±3.98和22.1±6.04(P>0.05);三个月后分别为13.30±4.12和15.70±5.07(P<0.05);6个月时分别为3.46±2.34和12.10±4.13(P<0.01),症状体征总积分明显优于HAART对照组。卡洛夫斯基积分中西医结合治疗组在疗后各月均优于对照组(P<0.05)。中西医结合治疗组和对照组CD4+T淋巴细胞计数起始为371.70±82.93和338.40±77.73(P>0.05);三个月后为414.33±81.77和360.63±77.01;六个月时476.40±87.50和408.80±78.87(P<0.05)有显著差异。CD8+T淋巴细胞计数、起始为1144.10±280.84和1154.20±350.36(P>0.05);三个月时为1309.90±307.52和1133.50±265.43六个月时为1424.50±425.10和1225.70±279.17(P<0.05)有显著差异。CD4/CD8、CD4+CD45RA组间比较无差异(P>0.05),两组病人HIV病毒载量检测治疗前后均组间组内比较均<500copies/ml。两组治疗前后组间比较安全性指标(血尿常规、X线、心电图、肝肾功能及血糖、血脂)均无统计学差异。
结论:中药益气健脾汤能够有效改善AIDS患者的临床症状体征,提高生活质量;对T淋巴细胞百分比无明显影响,但有效提升患者的CD4+T淋巴细胞计数,增强了患者的免疫功能;中西药合用未影响HAART抑制病毒复制的作用;未发现对细胞因子有显著影响。
1 Objective
Observed in patients before and after treatment of AIDS symptoms, signs, points change, quality of life impact. Indicators of T cell activation in peripheral blood CD4 + T lymphocytes, CD8 + T lymphocytes, CD4/CD8, CD4 + CD45RA, HIV viral load and IL-2, IL-10, TNF and other cytokines at different time points of change. Of the side effects on immune function, for in-depth study of Chinese medicine for HIV / AIDS impact of immune reconstitution to provide clinical basis.
2 Method
Strictly meet the test requirements of the AIDS screening 60 patients were already taking HAART drugs, based on randomly divided into two groups of 30 cases. Anti-HIV group were given two sides (AZT +3 TC + NVP) + Chinese and anti-HIV group side (AZT +3 TC + NVP). Two groups of patients were in the treatment of the first 0,3,6, months, signs of change in clinical symptoms, the CD4 + T lymphocytes, CD8 + T lymphocytes, CD4/CD8, CD4 + CD45RA, viral load testing, while the first month safety index 0,6 blood, urine, X ray, electrocardiogram, liver function, kidney function measurement.
3 Results
In the combination therapy group and the control group were initial symptom total score of 20.37±3.98 and 22.1±6.04 (P> 0.05); three months were 13.30±4.12 and 15.70±5.07 (P <0.05); 6 months was 3.46±2.34, respectively, and 12.10±4.13 (P <0.01), total score of symptoms and signs HAART was better than the control group. Carlo Minkowski integral combination therapy group were in each month after treatment than the control group (P <0.05). In the combination therapy group and the control group starting CD4 + T lymphocyte count was 371.70±82.93 and 338.40±77.73 (P> 0.05); three months after 414.33±81.77 and 360.63±77.01; six months 476.40±87.50 and 408.80±78.87 (P <0.05) were significantly different. CD8 + T lymphocyte count, starting at 1144.10±280.84 and 1154.20±350.36 (P> 0.05); three months to 1309.90±307.52 and 1133.50±265.43 1424.50±425.10 for the six months and 1225.70±279.17 (P < 0.05) were significantly different. CD4/CD8, CD4 + CD45RA no difference between the two groups (P> 0.05), HIV viral load in two groups of patients before and after treatment group were compared between the groups were <500copies/ml. Before and after treatment between the two groups safety index (urine routine, X ray, electrocardiogram, renal function and blood glucose, blood lipids) were not statistically different.
4 Conclusion
Spleen Qi decoction of Chinese medicine can improve clinical symptoms and signs of AIDS and improve the quality of life; on the percentage of T lymphocytes had no effect, but the effective increase in patients with CD4 + T lymphocyte count, and enhance the patient's immune function; Chinese and Western medicine Combined inhibition of viral replication did not affect the role of HAART; not found on the cell factor had significant effect.
引文
[1]国务院防治艾滋病委员会,卫生部,联合国艾滋病中国专题组.中国艾滋病防治联合评估报告.2009
[2]CarreNBoufassaFHubertJBetal.PredictivevalueofviralloadandothermarkersforprogressiontoclinicalAIDSafterCD4+cellcountfallsbelow200/microL.SEROCOHEMOCOStudyGrou[J].IntJEpidemiol,1998,27897-903.
[3] Haramati LB, Jenny-Avital ER, Alterman DD. Thoracic manifestations of immune restoration syndromes in AIDS[J]. Journal of Thoracic Imaging,2007,22(3):213-220.
[4] FIorence E,Lundgren J, Dreezen C, et al.Factors associated with a reduced CD4 Iymphocyte count response to HAART de-spite fuII viraI suppression in the Euro AIDS study.HIV Medi-cine,2003,4(3):255-262
[5]中华人民共和国卫生部. HIV/ A IDS诊断标准及处理原则.2001年颁布
[6] 2005年国家中医药管理局制定的《11省中医药治疗艾滋病临床技术方案》
[7] Autran B , Carcelain G, L i TS,et a l. Positive effects of combinedantiretroviral therapy on CD4+T cell homeostasis and function in advanced H IV disease [ J ]. Science, 1997, 277 ( 5322) : 112 116
[8]吴欣芳,王阶微生物与感染2009年3月第4卷第1期( 2009) 11 - 1874 - 03
[9] Ye P.Kirschner DE.Kourtis AP The thymus during HIV disease:role in pathogenesis and in immune recovery 2004(02)
[11] U.S. Food and Drug Administration.Drugs used in the treatment of HIV infection [EB/OL].(2007-10)[2008-1-12].http://www.fda.gov/oashi/ aids/ virals.html.
[12] Hengge UR , GoosM , Esser S,et a lII trial of subcutaneous interleukin 2 in combination w ith highly active antiretroviral treatment ( HAART) in H IV patients [ J ]. AIDS,1998, 12 ( 17) : 225.
[13]易玲,易莉.中医药治疗艾滋病研究进展[J].湖北中医杂志,2007,29(1):54.
[14]徐志明,李铭,和丽生.对艾滋病的探讨.云南中医学院学报,2000,23 (4):12
[15]胡建华,李秀惠,刘翠娥,等.100例艾滋病患者中医辨证分型研究[J].北京中医,2007,26(2):97-98.
[16]孙利民,危剑安,黄霞珍,等.从中医理论谈艾滋病的发病机制[ J ].中华中医药杂志, 2005, 20 ( 2) : 100 - 101.
[17]李发枝,徐立然,李伯龄.中医学对艾滋病病因病机的认识.中医杂志,2006,47(5) :395
[18]刘学伟.扶正排毒片对无症状H IV感染者早期干预作用的临床研究[ D ].郑州:河南中医学院第一临床医院, 2007.
[19]郭选贤,谢世平,孙林,等艾滋病中医病因病机研究概况[J]中医研究,2007,20(5):59
[20]王莉,段呈玉,瞿广城,等艾滋病的中医证候和演变规律的研究概况[J]云南中医中药杂志,2008,29(2):53
[21]何颖,浅析爱滋病的病因病机[J];湖北中医杂志; 2002年06期
[22]吕维柏.艾滋病中西医防治学[M].北京:人民卫生出版社,1994,144.
[23]吕维柏.中医药治疗HIV/AIDS血清抗体阴转8例报告[J].中国中西医结合杂志,1997,17(5):271-273.
[24]胡建华,李秀惠,刘翠娥,等.100例艾滋病患者中医辨证分型研究[J].北京中医,2007,26(2):97-98.
[25]方路,王莉,段吴玉,等.云南省180例HIV/AIDS的中医症状及证候初步分析[J].云南中医中药杂志,2006,27(3):38-39.
[26]张娴.方曙中药防治艾滋病研究进展-中国中医药信息杂志2000(01)
[27]王健,黄世敬.艾滋病中西医结合临床与研究[M ].北京:人民卫生出版社, 2007: 199.
[28]关崇芬.王健.徐淑玲中研2号治疗HIV/AIDS的临床和实验研究[J]-中国中西医结合杂志2003(07)
[29]李崇忠,李静.中医药治疗艾滋病理论的建立与临证应用.实用中医内科杂志,2002,12(6):62-63.
[30]何金祥等从肠道艾滋病毒感染机制试论艾滋病的中医辨治思路广州中医药大学学报2009
[31] Sanchez T H, Brooks J T, Sullivan P S, et alAdult/Adolescent Spectrum of HIV Disease Study Group:Bacterial diarrhea in persons with HIV infection, United States, 1992-2002[J]Clin Infect Dis, 2005,41(11):1621
[32]陈智.吴南屏.高效抗逆转录病毒治疗时代的免疫重建问题.浙江大学学报(医学版),2003,32:87-89.
[33]张兴权,范江.艾滋病病毒感染与艾滋病.人民卫生出版社,1999,108.
[34]李太生,邱志峰,王爱霞,等.HIV感染和AIDS患者T淋巴细胞免疫病理改变的研究.中华医学杂志,2002,82(20):1391-95.
[35]鲍作义,李林,庄道民等.HIV感染者CD4/CD8细胞与病毒载量的关系.中国公共卫生.2003,19(11):1380-1381.
[1] Lange CG, Valdez H , M edvik K,et a l. CD4+T Lymphocyte Nadir and the Effect of H ighly A ctive Antiretroviral Therapy on Pheno2. Random ized, controlled phaseinfected persons with detectable virusWANG J ieGuangan’2tor axis: understanding its central role in T cell homeostasis and thechallenges facing its utilization as a novel therapy [J]. Curr D rugKEY WORDS 21582.et al
[2]张天民试论抗艾滋病治疗后的免疫重建[J].临床肺科杂志, 2004, 9
[3]李太生Carcelaine G, Debre P,等高效抗病毒治疗促使艾滋病患者免疫功能重建[J].基础医学与临床2001,21(1):6-11.
[4]许利军,吴南屏. HIV感染后的免疫重建[J],国外医学:流行病学传染病学分册2003,30 (3):160[4]
[5]周华英HAART和HIV感染的免疫重建[J],2005,28
[6]Mocroft,Katlama,JohnsonAM,etal.AIDSacrossEurope,1994-1998:theEuroAIDAsstudy.Lancet,2000,356:291-296.
[7] Teixeira L VaIdez H,McCune JM,et al.Poor CD4 T ceII res-toratron after suppression of HIV-1 repIication may refIect Iower thymic function.AIDS,2001,15(14):1749-1756
[8] Easterbrook PJ, Yu LM,Goetyhebeur E,et al.Ten-year trends in CD4 cell counts at HIV and AIDS diagnosis in a London HIV clinic [J].AIDS, 2000,14(5):561-571
[9] Ye P.Kirschner DE.Kourtis AP The thymus during HIV disease:role in pathogenesis and in immune recovery 2004(02)
[10] Kalams SA.Goulder PJ.Shea AK Levels of human immunodeficiency virus type-1 specific cytotoxic T-lymphocyte effector and memory response decline after suppression of viremia with highly active antiretroviral therapy 1999(08)
[11] Wilde MI, Langtry HD. Zidovudine. An update of its pharmacodynamic and pharmacokinetic properties, and therapeutic efficacy. Drugs 1993,46:515–78.
[12] Drake S M. NNRTIs - a new class of drugs for HIV. J Antimicrob Chemother,2000, 45(4):417.
[13]周伟,危剑安,孙利民等.AIDS的高效抗逆转录病毒疗法的毒副作用.中国艾滋病性病,2005,11(1):73-75.
[14] Imam i N , Gotch F. Prospects for immune reconstitution in H IV 1 infection [J]. Clin Exp Immunol, 2002, 127 ( 3) : 402–411
[15] Hengge UR , GoosM , Esser S,et a lII trial of subcutaneous interleukin 2 in combination w ith highly active antiretroviral treatment ( HAART) in H IV patients [ J ]. A IDS,1998, 12 ( 17) : 225.
[16] Tambussi G, Ghezzi S, Nozza S,et a l. Efficacy of low dose interm ittent subcutaneous interleukin (IL) 2 in antiviral drug experiencedhuman immunodeficiency virusload: a controlled study of 3 ILTargets, 2006, 7 (12) : 1571
[17] Lu H , Zhao Z, Kalina T Vaccine, regimens w ith antiviral drug therapy [J]. J Infect Dis, 2001, 183 (10) : 1467–1484
[18] Sasson SC, Zaunders JJ, Kelleher AD ,et a l. The IL 7 / IL 7 recep.Interleukin 7 imp roves reconstituT cells [J]. Clin Immunol, 2005, 114.
[19] 1 M aggiolo F, R ipamonti D , Gregis G,et a l1Effect of p ro longeddiscontinuation of successful antiretroviral therapy on CD4 T cells: a contro lled, p ro spective tria AIDS, 2004 , 18 (3)439 446
[20] Carcelain G, Tubiana R , Sam ri A,etal. Transient mobilization of humanimmunodeficiency virus ( H IV ) specific CD4 T-helper Cells: fails to contro l virus rebounds during interm ittent antiretroviral therapy in chronic H IV type 1 infection 2001 , 75(1)234-341
[21] Fischer M , Hafner R Schneider c ,et a H IV RNA in p lasma1et a lbounds wthin days during structured treatment interrup tions AIDS, 2003, 17 (2)
[22] Law rence J, M ayers DL , Hullsiek KH et a Structured treatment interrup tion in patients w ith multidrug resistant human im munodeficiency virus N Engl J M ed, 2003, 349 (9) : 837)-846
[23] Rosenberg ES, A ltfeld M , Poon SH ,et a lImmune control of H IV1 after early treatment of acute infection [J]. Nature, 2000, 407
[24] Moss RB , B randt C, Giermakow ska W K,et a l. H IV specific immunity during structured antiviral drug treatment interrup tion [J].12) : 1066 1071
[25] Fischer M , Hafner R , Schneider C,et a l. H IV RNA in p lasma rebounds w ithin days during structured treatment interrup tions [ J ].
[26] Oxenius A , Price DA , Gunthard HF,et a l. Stimulation of H IVspecific cellular immunity by structured treatment interrup tion fails toUSA [J]. 2002, 99
[27] Ruiz L , Carcelain G, M artinez2de Loes S. Role of therapeutic vaccines in the control of typ ic and Functional Immune Restoration in H IVClin Immunol, 2002, 102 (2) : 154typ ic and Functional Immune Restoration in H IVClin Immunol, 2002, 102 (2) : 154
[28] Little K,Surjadi M.A scientific overview of the development of AIDS vaccines.J Assoc Nurses AIDS Care,2000,11:19~28Invest. 1981.68(6):1548—1557.
[29]易玲,易莉.中医药治疗艾滋病研究进展[J].湖北中医杂志,2007,29(1):54.
[30]屈冰.试述艾滋病的病因病机.中医研究,2006,19 (5) :10
[31]杨凤珍,烟建华,王健,等.HIV/AIDS中医分期辨证治疗.中国医药学报,2004,19(4) :240
[32]李发枝,徐立然,李伯龄.中医学对艾滋病病因病机的认识.中医杂志,2006,47(5) :395
[33]刘学伟,郭会军,刘琦,等.艾滋病从“毒邪”论治探析.中医杂志,2006,47( 11) : 803
[34]彭勃,苗明三,杨晓娜中医药治疗艾滋病的研究进展[J]河南中医, 2006,21(1)82
[35]郭选贤,谢世平,孙林,等艾滋病中医病因病机研究概况[J]中医研究,2007,20(5):59
[36]王莉,段呈玉,瞿广城,等艾滋病的中医证候和演变规律的研究概况[J]云南中医中药杂志,2008,29(2):53
[37]何颖;浅析爱滋病的病因病机[J];湖北中医杂志; 2002年06期
[38]吕维柏.艾滋病中西医防治学[M].北京:人民卫生出版社,1994,144.
[39]吕维柏.中医药治疗HIV/AIDS血清抗体阴转8例报告[J].中国中西医结合杂志,1997,17(5):271-273.
[40]赵晓梅.490例HIV感染者的流行病学及证候学分析[J].中国中医基础医学杂志,1995,1(4):38.
[41]胡建华,李秀惠,刘翠娥,等.100例艾滋病患者中医辨证分型研究[J].北京中医,2007,26(2):97-98.
[42]邱红,谢世平,郭选贤.HIV/AIDS 274例中医证候流行病学分布[J].郑州大学学报(医学版),2007,42(2):363-364.
[43]李洪娟,李峰,王健,等.158例HIV/AIDS感染者常见中医症状和证候分析[J].北京中医药大学学报,2005,28(1):69-72.
[44]方路,王莉,段吴玉,等.云南省180例HIV/AIDS的中医症状及证候初步分析[J].云南中医中药杂志,2006,27(3):38-39.
[45]吕乃达,王禄林.艾滋病的中医药治疗研究[J].中医药临床杂志, 2005,17(4) :409 .
[46]王艳艳,张晓静,王桂英.中药单体及复方制剂抗艾滋病的研究进展[J].中医研究,2004,17(16):5960.
[47]刘因华,曹志红.抗艾滋病药物的发展近况[ J ] .云南中医中药杂志,2004,25(6):4445.
[48]贺金华,买尔旦.中药防治艾滋病研究概况[J].中国药物与临床,2004,4(6):454456.
[49] Xia X,Lai Z,Cao X,et al. A preliminary clinical evaluation of treatment with the traditional Chinese medicine‘XIN XUE PIAN’in HCVcoinfected AIDS patients[J].Antiviral Ther,2003,8:500.
[50]朱善岚,陈应振,陈子榕,等.中药治疗艾滋病概述[J].海峡药学,2001,13(2) :104 .
[51]谢世平,郭会军,许前磊,等.爱康胶囊对HIV / AIDS患者生存质量的影响[J].上海中医药杂志,2007,41(9) :15 .
[52]李发枝,徐立然,李伯龄,等.中医药对173例艾滋病患者T淋巴细胞亚群的影响[J].中医杂志,2006,47(1) :31 -32 .
[53]王健,黄世敬.艾滋病中西医结合临床与研究[M ].北京:人民卫生出版社, 2007: 199.
[54]吕维柏.中医药治疗艾滋病的最新进展和对策[J].河南中医学院学报, 2004,19 (11):14.
[55]尹勇,段丽萍,刘玉生.针灸治疗艾滋病23例[J].上海中医药大学学报,2002,16(2):29-30.
[56]赵丹,李峰,张宁.中医非药物疗法防治艾滋病研究进展[J].北京中医药大学学报,2006,29(1):63-66.
[57]何庆年.略论气功抗肿瘤与艾滋病[J].东方气功,1990,(1):12-15.
[58]李朝武,陈玉平,胡金銮.浅析中医药和气功可控制艾滋病感染期的发展[J].中医药动态,1993,(1):12.
[59]庞俊清.气功—艾滋病患者希望之光[J]气功与科学,1992,(12):46.
[60]刘振杰,胡伟飚.艾滋病的中医药研究进展[J].山东中医杂志,2006,25(4)228 .
[61]周立华,唐英,卢依平,等.艾灸法治疗艾滋病脾气虚腹泻的临床研究[J].上海针灸杂志,2008,27(5),19 .
[62]王素秋,党斌温,燕相山,等.艾滋病及其肺合并症3例分析[J].
[63]杨凤珍,王健,赵敏.72例HIV/AIDS患者中医证候与T淋巴细胞亚群和病毒载量相关性研究.中国医药学报.2004,19(12):733-737.
[64]张可,王健,蒋岩等.中药治疗200例HIV感染者/AIDS患者的结果分析.中国艾滋病性病.2005,11(2):94-96.
[65]李发枝,徐立然,李柏龄.中医药对173例艾滋病患者T淋巴细胞亚群的影响中医杂志.2006,47(1):31-32.
[66]危剑安,孙利民,陈宇霞.中药艾灵颗粒对IV/AIDS患者免疫重建的影响.中国中西医结合杂志.2006,26(4):319-311.
[67]刘振杰,胡伟飚.艾滋病的中医药研究进展.山东中医杂志.2006,25(4):228- 231.
[68]耿文清,尚红,姜拥军.经血感染HIV/AIDS患者pDCs与艾滋病疾病进展关系的研究.中华微生物学和免疫学杂志.2005,25(8):663-666.
[69]高月求,郑亚江,王灵台.健脾方对脾虚型慢性乙型肝炎患者树突细胞功能的影响.中国中西医结合杂志.2007,27(4):300-303.
[2]CarreNBoufassaFHubertJBetal.PredictivevalueofviralloadandothermarkersforprogressiontoclinicalAIDSafterCD4+cellcountfallsbelow200/microL.SEROCOHEMOCOStudyGrou[J].IntJEpidemiol,1998,27897-903.
[3] Haramati LB, Jenny-Avital ER, Alterman DD. Thoracic manifestations of immune restoration syndromes in AIDS[J]. Journal of Thoracic Imaging,2007,22(3):213-220.
[4] FIorence E,Lundgren J, Dreezen C, et al.Factors associated with a reduced CD4 Iymphocyte count response to HAART de-spite fuII viraI suppression in the Euro AIDS study.HIV Medi-cine,2003,4(3):255-262
[5]中华人民共和国卫生部. HIV/ A IDS诊断标准及处理原则.2001年颁布
[6] 2005年国家中医药管理局制定的《11省中医药治疗艾滋病临床技术方案》
[7] Autran B , Carcelain G, L i TS,et a l. Positive effects of combinedantiretroviral therapy on CD4+T cell homeostasis and function in advanced H IV disease [ J ]. Science, 1997, 277 ( 5322) : 112 116
[8]吴欣芳,王阶微生物与感染2009年3月第4卷第1期( 2009) 11 - 1874 - 03
[9] Ye P.Kirschner DE.Kourtis AP The thymus during HIV disease:role in pathogenesis and in immune recovery 2004(02)
[11] U.S. Food and Drug Administration.Drugs used in the treatment of HIV infection [EB/OL].(2007-10)[2008-1-12].http://www.fda.gov/oashi/ aids/ virals.html.
[12] Hengge UR , GoosM , Esser S,et a lII trial of subcutaneous interleukin 2 in combination w ith highly active antiretroviral treatment ( HAART) in H IV patients [ J ]. AIDS,1998, 12 ( 17) : 225.
[13]易玲,易莉.中医药治疗艾滋病研究进展[J].湖北中医杂志,2007,29(1):54.
[14]徐志明,李铭,和丽生.对艾滋病的探讨.云南中医学院学报,2000,23 (4):12
[15]胡建华,李秀惠,刘翠娥,等.100例艾滋病患者中医辨证分型研究[J].北京中医,2007,26(2):97-98.
[16]孙利民,危剑安,黄霞珍,等.从中医理论谈艾滋病的发病机制[ J ].中华中医药杂志, 2005, 20 ( 2) : 100 - 101.
[17]李发枝,徐立然,李伯龄.中医学对艾滋病病因病机的认识.中医杂志,2006,47(5) :395
[18]刘学伟.扶正排毒片对无症状H IV感染者早期干预作用的临床研究[ D ].郑州:河南中医学院第一临床医院, 2007.
[19]郭选贤,谢世平,孙林,等艾滋病中医病因病机研究概况[J]中医研究,2007,20(5):59
[20]王莉,段呈玉,瞿广城,等艾滋病的中医证候和演变规律的研究概况[J]云南中医中药杂志,2008,29(2):53
[21]何颖,浅析爱滋病的病因病机[J];湖北中医杂志; 2002年06期
[22]吕维柏.艾滋病中西医防治学[M].北京:人民卫生出版社,1994,144.
[23]吕维柏.中医药治疗HIV/AIDS血清抗体阴转8例报告[J].中国中西医结合杂志,1997,17(5):271-273.
[24]胡建华,李秀惠,刘翠娥,等.100例艾滋病患者中医辨证分型研究[J].北京中医,2007,26(2):97-98.
[25]方路,王莉,段吴玉,等.云南省180例HIV/AIDS的中医症状及证候初步分析[J].云南中医中药杂志,2006,27(3):38-39.
[26]张娴.方曙中药防治艾滋病研究进展-中国中医药信息杂志2000(01)
[27]王健,黄世敬.艾滋病中西医结合临床与研究[M ].北京:人民卫生出版社, 2007: 199.
[28]关崇芬.王健.徐淑玲中研2号治疗HIV/AIDS的临床和实验研究[J]-中国中西医结合杂志2003(07)
[29]李崇忠,李静.中医药治疗艾滋病理论的建立与临证应用.实用中医内科杂志,2002,12(6):62-63.
[30]何金祥等从肠道艾滋病毒感染机制试论艾滋病的中医辨治思路广州中医药大学学报2009
[31] Sanchez T H, Brooks J T, Sullivan P S, et alAdult/Adolescent Spectrum of HIV Disease Study Group:Bacterial diarrhea in persons with HIV infection, United States, 1992-2002[J]Clin Infect Dis, 2005,41(11):1621
[32]陈智.吴南屏.高效抗逆转录病毒治疗时代的免疫重建问题.浙江大学学报(医学版),2003,32:87-89.
[33]张兴权,范江.艾滋病病毒感染与艾滋病.人民卫生出版社,1999,108.
[34]李太生,邱志峰,王爱霞,等.HIV感染和AIDS患者T淋巴细胞免疫病理改变的研究.中华医学杂志,2002,82(20):1391-95.
[35]鲍作义,李林,庄道民等.HIV感染者CD4/CD8细胞与病毒载量的关系.中国公共卫生.2003,19(11):1380-1381.
[1] Lange CG, Valdez H , M edvik K,et a l. CD4+T Lymphocyte Nadir and the Effect of H ighly A ctive Antiretroviral Therapy on Pheno2. Random ized, controlled phaseinfected persons with detectable virusWANG J ieGuangan’2tor axis: understanding its central role in T cell homeostasis and thechallenges facing its utilization as a novel therapy [J]. Curr D rugKEY WORDS 21582.et al
[2]张天民试论抗艾滋病治疗后的免疫重建[J].临床肺科杂志, 2004, 9
[3]李太生Carcelaine G, Debre P,等高效抗病毒治疗促使艾滋病患者免疫功能重建[J].基础医学与临床2001,21(1):6-11.
[4]许利军,吴南屏. HIV感染后的免疫重建[J],国外医学:流行病学传染病学分册2003,30 (3):160[4]
[5]周华英HAART和HIV感染的免疫重建[J],2005,28
[6]Mocroft,Katlama,JohnsonAM,etal.AIDSacrossEurope,1994-1998:theEuroAIDAsstudy.Lancet,2000,356:291-296.
[7] Teixeira L VaIdez H,McCune JM,et al.Poor CD4 T ceII res-toratron after suppression of HIV-1 repIication may refIect Iower thymic function.AIDS,2001,15(14):1749-1756
[8] Easterbrook PJ, Yu LM,Goetyhebeur E,et al.Ten-year trends in CD4 cell counts at HIV and AIDS diagnosis in a London HIV clinic [J].AIDS, 2000,14(5):561-571
[9] Ye P.Kirschner DE.Kourtis AP The thymus during HIV disease:role in pathogenesis and in immune recovery 2004(02)
[10] Kalams SA.Goulder PJ.Shea AK Levels of human immunodeficiency virus type-1 specific cytotoxic T-lymphocyte effector and memory response decline after suppression of viremia with highly active antiretroviral therapy 1999(08)
[11] Wilde MI, Langtry HD. Zidovudine. An update of its pharmacodynamic and pharmacokinetic properties, and therapeutic efficacy. Drugs 1993,46:515–78.
[12] Drake S M. NNRTIs - a new class of drugs for HIV. J Antimicrob Chemother,2000, 45(4):417.
[13]周伟,危剑安,孙利民等.AIDS的高效抗逆转录病毒疗法的毒副作用.中国艾滋病性病,2005,11(1):73-75.
[14] Imam i N , Gotch F. Prospects for immune reconstitution in H IV 1 infection [J]. Clin Exp Immunol, 2002, 127 ( 3) : 402–411
[15] Hengge UR , GoosM , Esser S,et a lII trial of subcutaneous interleukin 2 in combination w ith highly active antiretroviral treatment ( HAART) in H IV patients [ J ]. A IDS,1998, 12 ( 17) : 225.
[16] Tambussi G, Ghezzi S, Nozza S,et a l. Efficacy of low dose interm ittent subcutaneous interleukin (IL) 2 in antiviral drug experiencedhuman immunodeficiency virusload: a controlled study of 3 ILTargets, 2006, 7 (12) : 1571
[17] Lu H , Zhao Z, Kalina T Vaccine, regimens w ith antiviral drug therapy [J]. J Infect Dis, 2001, 183 (10) : 1467–1484
[18] Sasson SC, Zaunders JJ, Kelleher AD ,et a l. The IL 7 / IL 7 recep.Interleukin 7 imp roves reconstituT cells [J]. Clin Immunol, 2005, 114.
[19] 1 M aggiolo F, R ipamonti D , Gregis G,et a l1Effect of p ro longeddiscontinuation of successful antiretroviral therapy on CD4 T cells: a contro lled, p ro spective tria AIDS, 2004 , 18 (3)439 446
[20] Carcelain G, Tubiana R , Sam ri A,etal. Transient mobilization of humanimmunodeficiency virus ( H IV ) specific CD4 T-helper Cells: fails to contro l virus rebounds during interm ittent antiretroviral therapy in chronic H IV type 1 infection 2001 , 75(1)234-341
[21] Fischer M , Hafner R Schneider c ,et a H IV RNA in p lasma1et a lbounds wthin days during structured treatment interrup tions AIDS, 2003, 17 (2)
[22] Law rence J, M ayers DL , Hullsiek KH et a Structured treatment interrup tion in patients w ith multidrug resistant human im munodeficiency virus N Engl J M ed, 2003, 349 (9) : 837)-846
[23] Rosenberg ES, A ltfeld M , Poon SH ,et a lImmune control of H IV1 after early treatment of acute infection [J]. Nature, 2000, 407
[24] Moss RB , B randt C, Giermakow ska W K,et a l. H IV specific immunity during structured antiviral drug treatment interrup tion [J].12) : 1066 1071
[25] Fischer M , Hafner R , Schneider C,et a l. H IV RNA in p lasma rebounds w ithin days during structured treatment interrup tions [ J ].
[26] Oxenius A , Price DA , Gunthard HF,et a l. Stimulation of H IVspecific cellular immunity by structured treatment interrup tion fails toUSA [J]. 2002, 99
[27] Ruiz L , Carcelain G, M artinez2de Loes S. Role of therapeutic vaccines in the control of typ ic and Functional Immune Restoration in H IVClin Immunol, 2002, 102 (2) : 154typ ic and Functional Immune Restoration in H IVClin Immunol, 2002, 102 (2) : 154
[28] Little K,Surjadi M.A scientific overview of the development of AIDS vaccines.J Assoc Nurses AIDS Care,2000,11:19~28Invest. 1981.68(6):1548—1557.
[29]易玲,易莉.中医药治疗艾滋病研究进展[J].湖北中医杂志,2007,29(1):54.
[30]屈冰.试述艾滋病的病因病机.中医研究,2006,19 (5) :10
[31]杨凤珍,烟建华,王健,等.HIV/AIDS中医分期辨证治疗.中国医药学报,2004,19(4) :240
[32]李发枝,徐立然,李伯龄.中医学对艾滋病病因病机的认识.中医杂志,2006,47(5) :395
[33]刘学伟,郭会军,刘琦,等.艾滋病从“毒邪”论治探析.中医杂志,2006,47( 11) : 803
[34]彭勃,苗明三,杨晓娜中医药治疗艾滋病的研究进展[J]河南中医, 2006,21(1)82
[35]郭选贤,谢世平,孙林,等艾滋病中医病因病机研究概况[J]中医研究,2007,20(5):59
[36]王莉,段呈玉,瞿广城,等艾滋病的中医证候和演变规律的研究概况[J]云南中医中药杂志,2008,29(2):53
[37]何颖;浅析爱滋病的病因病机[J];湖北中医杂志; 2002年06期
[38]吕维柏.艾滋病中西医防治学[M].北京:人民卫生出版社,1994,144.
[39]吕维柏.中医药治疗HIV/AIDS血清抗体阴转8例报告[J].中国中西医结合杂志,1997,17(5):271-273.
[40]赵晓梅.490例HIV感染者的流行病学及证候学分析[J].中国中医基础医学杂志,1995,1(4):38.
[41]胡建华,李秀惠,刘翠娥,等.100例艾滋病患者中医辨证分型研究[J].北京中医,2007,26(2):97-98.
[42]邱红,谢世平,郭选贤.HIV/AIDS 274例中医证候流行病学分布[J].郑州大学学报(医学版),2007,42(2):363-364.
[43]李洪娟,李峰,王健,等.158例HIV/AIDS感染者常见中医症状和证候分析[J].北京中医药大学学报,2005,28(1):69-72.
[44]方路,王莉,段吴玉,等.云南省180例HIV/AIDS的中医症状及证候初步分析[J].云南中医中药杂志,2006,27(3):38-39.
[45]吕乃达,王禄林.艾滋病的中医药治疗研究[J].中医药临床杂志, 2005,17(4) :409 .
[46]王艳艳,张晓静,王桂英.中药单体及复方制剂抗艾滋病的研究进展[J].中医研究,2004,17(16):5960.
[47]刘因华,曹志红.抗艾滋病药物的发展近况[ J ] .云南中医中药杂志,2004,25(6):4445.
[48]贺金华,买尔旦.中药防治艾滋病研究概况[J].中国药物与临床,2004,4(6):454456.
[49] Xia X,Lai Z,Cao X,et al. A preliminary clinical evaluation of treatment with the traditional Chinese medicine‘XIN XUE PIAN’in HCVcoinfected AIDS patients[J].Antiviral Ther,2003,8:500.
[50]朱善岚,陈应振,陈子榕,等.中药治疗艾滋病概述[J].海峡药学,2001,13(2) :104 .
[51]谢世平,郭会军,许前磊,等.爱康胶囊对HIV / AIDS患者生存质量的影响[J].上海中医药杂志,2007,41(9) :15 .
[52]李发枝,徐立然,李伯龄,等.中医药对173例艾滋病患者T淋巴细胞亚群的影响[J].中医杂志,2006,47(1) :31 -32 .
[53]王健,黄世敬.艾滋病中西医结合临床与研究[M ].北京:人民卫生出版社, 2007: 199.
[54]吕维柏.中医药治疗艾滋病的最新进展和对策[J].河南中医学院学报, 2004,19 (11):14.
[55]尹勇,段丽萍,刘玉生.针灸治疗艾滋病23例[J].上海中医药大学学报,2002,16(2):29-30.
[56]赵丹,李峰,张宁.中医非药物疗法防治艾滋病研究进展[J].北京中医药大学学报,2006,29(1):63-66.
[57]何庆年.略论气功抗肿瘤与艾滋病[J].东方气功,1990,(1):12-15.
[58]李朝武,陈玉平,胡金銮.浅析中医药和气功可控制艾滋病感染期的发展[J].中医药动态,1993,(1):12.
[59]庞俊清.气功—艾滋病患者希望之光[J]气功与科学,1992,(12):46.
[60]刘振杰,胡伟飚.艾滋病的中医药研究进展[J].山东中医杂志,2006,25(4)228 .
[61]周立华,唐英,卢依平,等.艾灸法治疗艾滋病脾气虚腹泻的临床研究[J].上海针灸杂志,2008,27(5),19 .
[62]王素秋,党斌温,燕相山,等.艾滋病及其肺合并症3例分析[J].
[63]杨凤珍,王健,赵敏.72例HIV/AIDS患者中医证候与T淋巴细胞亚群和病毒载量相关性研究.中国医药学报.2004,19(12):733-737.
[64]张可,王健,蒋岩等.中药治疗200例HIV感染者/AIDS患者的结果分析.中国艾滋病性病.2005,11(2):94-96.
[65]李发枝,徐立然,李柏龄.中医药对173例艾滋病患者T淋巴细胞亚群的影响中医杂志.2006,47(1):31-32.
[66]危剑安,孙利民,陈宇霞.中药艾灵颗粒对IV/AIDS患者免疫重建的影响.中国中西医结合杂志.2006,26(4):319-311.
[67]刘振杰,胡伟飚.艾滋病的中医药研究进展.山东中医杂志.2006,25(4):228- 231.
[68]耿文清,尚红,姜拥军.经血感染HIV/AIDS患者pDCs与艾滋病疾病进展关系的研究.中华微生物学和免疫学杂志.2005,25(8):663-666.
[69]高月求,郑亚江,王灵台.健脾方对脾虚型慢性乙型肝炎患者树突细胞功能的影响.中国中西医结合杂志.2007,27(4):300-303.
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