用户名: 密码: 验证码:
土壤基本养分及其它无机元素对川白芷产量和质量的影响研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
本文对川白芷道地产区四川省遂宁市各乡镇15个不同采集地的川白芷根部农艺性状、欧前胡素和异欧前胡素含量,以及相应生长地土壤基本养分和其它11种无机元素进行测定,通过相关分析研究土壤基本养分及其它无机元素对川白芷产量及质量的影响;同时将经过多年选育的10个不同白芷品系,在四川省雅安市和遂宁市分别同田栽培,考察其各自产量、欧前胡素和异欧前胡素含量。研究的主要结果如下:
     1.遂宁不同川白芷生长地土壤基本养分差异明显。所有土壤均为弱碱性,pH值变幅8.49-8.88,平均8.68;有机质含量普遍偏低,变幅10.15-25.16 g/kg,平均15.12 g/kg;全氮含量水平普遍偏低,变幅0.72-1.68 g/kg,平均0.99 g/kg;而碱解氮含量则处于较高水平,变幅63.87-144.6 mg/kg,平均96.88 mg/kg;有效磷含量处于较高水平,变幅9.45-38.01 g/kg,平均22.56 g/kg;速效钾含量处于适中水平,变幅32.28-138.4 mg/kg,平均68.93 mg/kg。
     2.遂宁不同川白芷生长地土壤中10种其它无机元素含量间也存在明显差异。其中,Ca的含量变化范围为19788.92-65651.16 mg/kg,平均51143.05 mg/kg;Fe的含量变化范围为24410.62-35147.12 mg/kg,平均27786.65 mg/kg;Mn的含量变化范围为248.25-674.99 mg/kg,平均558.73 mg/kg;Mg的含量变化范围为5986.33-17499.63 mg/kg,平均10636.64 mg/kg;Pb的含量变化范围为3.99-7.02mg/kg,平均5.67 mg/kg;As的含量变化范围为1.50-5.86 mg/kg,平均3.33 mg/kg;Zn的含量变化范围为29.53-83.76 mg/kg,平均59.71 mg/kg;Cr的含量变化范围为5.50-14.00 mg/kg,平均9.43 mg/kg;Cu的含量变化范围为12.53-25.13 mg/kg,平均18.40 mg/kg;Hg的含量变化范围为0.03-0.19 mg/kg,平均0.10 mg/kg。未检测到Cd。
     3.遂宁不同生长地川白芷的主要农艺性状、欧前胡素和异欧前胡素含量以及9种无机元素含量也不尽相同。其中,根重变幅10.60-23.25 g,平均16.36 g;根长变幅9.70-24.00 cm,平均18.75 cm;根头直径变幅3.90-9.25 cm,平均6.96 cm;欧前胡素含量变幅1.29-5.62 mg/g,平均2.48 mg/g;异欧前胡素含量变幅0.46-1.37mg/g,平均0.88 mg/g。Ca含量变幅6423.92-10664.04 mg/kg,平均7928.53 mg/kg;Mg含量变幅2496.01-3499.30 mg/kg,平均2906.39 mg/kg;Fe含量变幅287.21-1423.53 mg/kg,平均728.78 mg/kg;Mn含量变幅17.73-69.39 mg/kg,平均31.65mg/kg;Cr含量变幅9.00-36.69 mg/kg,平均17.15 mg/kg;Zn含量变幅6.91-12.91mg/kg,平均9.41 mg/kg;Cu含量变幅0.75-1.75 mg/kg,平均1.34 mg/kg;Pb含量变幅0.25-1.75 mg/kg,平均0.71 mg/kg;As含量变幅0.18-1.09 mg/kg,平均3.37 mg/kg。未检测到Cd和Hg。
     4.相关分析结果表明,川白芷根长与土壤有效磷含量、根头直径与土壤有机质含量、根重与土壤有效磷及速效钾含量间均呈显著正相关,土壤全氮则与川白芷异欧前胡素含量间呈显著负相关。川白芷欧前胡素和异欧前胡素含量与土壤中其它11种无机元素含量间相关均不显著。由此,土壤中氮、磷和钾与川白芷产量和质量密切相关,生产中应注意氮、磷和钾肥的施用。
     5.土壤中各无机元素与川白芷中无机元素间相关均不显著,揭示了川白芷可能通过主动吸收调控药材中无机元素的含量;川白芷中钙与铁、钙与锰、钙与铅、钙与砷、铁与锰、铁与锌、锰与砷极显著相关,钙与锌、铁与铅、铁与砷、锰与铅、铅与锌、铅与铜显著相关。由此,川白芷钙、铁、锰、锌、铅、铜与大部分元素间的吸收有很好的协同作用。
     6.将经多年选育得到的10个川白芷品系在雅安同田种植,结果发现不同品系各性状间存在显著差异。其根长变幅15.38-29.36 cm,平均22.04 cm;根头直径变幅2.10-3.94 cm,平均3.16 cm;小区产量变幅4.7-9.7 kg,平均7.3 kg;欧前胡素变幅0.997-2.516 mg/g,平均1.739 mg/g;异欧前胡素含量变幅0.473-1.036mg/g,平均0.736 mg/g。上述10个不同川白芷品系在遂宁同田种植,其各性状间差异也达显著水平。根长变幅21.77-33.29 cm,平均26.23 cm;根头直径变幅2.94-4.37 cm,平均3.57 cm,小区产量变幅17.0-23.5 kg,平均20.40 kg;欧前胡素含量变幅1.671-2.721 mg/g,平均2.250 mg/g;异欧前胡素含量变幅0.644-1.458mg/g,平均1.075 mg/g。说明通过选择仍可以得到性状差异明显的不同川白芷品系。
     7.比较雅安和遂宁两个试验点川白芷品系主要农艺性状和有效成分含量。不难发现,遂宁种植的川白芷品系比雅安种植的相同川白芷品系根长均长,平均长4.19cm;根粗也明显,平均粗0.41 cm;小区产量均高于相应品系,平均增产13.1kg,是雅安种植的川白芷品系小区产量的2.79倍,欧前胡素和异欧前胡素含量则分别比雅安的高0.521 mg/g和0.339 mg/g。进一步说明环境对川白芷的产量质量影响确实很大,遂宁的土壤气候等环境更利于川白芷优质高产。
To study the effects of the basic soil nutrients and other 11 different inorganic elements on yield and quality of Angelica dahurica originated from Sichuan Province.The main agronomic traits on the yield and the content of imperatorin, isoimperatorin and other 11 different inorganic elements of A. dahurica gathered at 15 different spots of Suining in Sichuan Province was determined. And the basic soil nutrients and other 11 different mineral elements of the soil were analyzed. The effects of the basic soil nutrients and other 11 different inorganic elements on the yield and quality were analyzed through correlation analysis. At the same time,10 different A. dahurcia screened strains were cultivated under the same soil conditions in Yaan and Suining respectively to explore the yield, content of imperatorin and isoimperatorin of them. The main results are as follows:
     1. The difference of basic soil nutrients of different spots A. dahurica grew in was obvious in Suining. All the soil were weak alkaline, pH values ranged from 8.49 to 8.88, 8.68 in average. Organic matter contents were generally low, ranged from 10.15 to 25.16 g/kg, with the average of 15.12 g/kg. Total nitrogen levels were generally low, ranged from 0.72 to 1.68 g/kg,0.99 g/kg in average. The nitrogen contents were at a high level, ranged from 63.87 to 144.6 mg/kg, with an average of 96.88 mg/kg. Phosphorus contents were at a high level, ranged from 9.45 to 38.01g/kg,22.56 g/kg in average. Available potassium were in the moderate level, ranged from 32.28 to 138.4 mg/kg,68.93 mg/kg in average.
     2. The difference of other 10 different mineral elements of the different spots of soil A.dahurica grew in was also obvious at Suining. Of them, the content of Ca ranged from 19788.92 to 65651.16 mg/kg,51143.05 mg/kg in average. The content of Fe ranged from 24410.62 to 35147.12 mg/kg,27786.65 mg/kg in average. The content of Mn ranged from 248.25 to 674.99 mg/kg,558.73 mg/kg in average. The content of Mg ranged from 5986.33 to 17499.63 mg/kg,10636.64 mg/kg in average. The content of Pb ranged from 3.99 to 7.02 mg/kg,5.67 mg/kg in average. The content of As ranged from 1.50 to 5.86 mg/kg,3.33 mg/kg in average. The content of Zn ranged from 29.53 to 83.76 mg/kg, 59.71 mg/kg in average. The content of Cr ranged from 5.50 to 14.00 mg/kg,9.43 mg/kg in average. The content of Cu ranged from 12.53 to 25.13 mg/kg,18.40 mg/kg in average. The content of Hg ranged from 0.03 to 0.19 mg/kg,0.10 mg/kg in average. Cd could not be detected.
     3.The main agronomic traits of root, content of imperatorin, isoimperatorin and other 9 different mineral elements of the different spots of soil A. dahurica grew in were different in Suining. Of them, The root weights ranged from 10.60 to 23.25 g,16.36 g in average. Lengths ranged from 9.70 to 24.00 cm,18.75 cm in average. Circumferences ranged from 3.90 to 9.25 cm,6.96 cm in average. And the contents of imperatorin ranged from 1.29 to 5.62 mg/g,2.48 mg/g in average. Isoimperatorin contents ranged from 0.46 to 1.37 mg/g,0.88 mg/g in average. The contents of Ca ranged from 6423.92 to 10664.04 mg/kg,7928.53 mg/kg in average. The contents of Mg ranged from 2496.01 to 3499.30 mg/kg,2906.39 mg/kg in average. The contents of Fe ranged from 287.21 to 1423.53 mg/kg,728.78 mg/kg in average. The contents of Mn ranged from 17.73 to 69.39 mg/kg, 31.65 mg/kg in average. The contents of Cr ranged from 9.00 to 36.69 mg/kg,17.15 mg/kg in average. The contents of Zn ranged from 6.91 to 12.91 mg/kg,9.41 mg/kg in average. The contents of Cu ranged from 0.75 to 1.75 mg/kg,1.34 mg/kg in average; The contents of Pb ranged from 0.25 to 1.75 mg/kg,0.71 mg/kg in average. Cd and Hg could not be detected.
     4. Correlation analysis showed that the positive correlation between root length of A. dahurica and soil available P content, root circumference of A. dahurica and soil organic matter content, root weight of A. dahurica and soil available P and K contents were all significant. The soil total nitrogen content had negatively significant corratation with the content of isoimperatorin. The correlation between the imperatorin, isoimperatorin contents of A. dahurica and 11 other soil inorganicel elements were not significant. This showed that N, P and K content of soil had obvious relationship with the yield and quality of A. dahurica from Sichuan province. We should pay attention to manure N, P and K in production.
     5. The correlation between the soil inorganic elements and inorganic elements of Angelica dahurica were not significant. This showed that A. dahurica could regulated the content of inorganic elements through active absorption. The correlation between Ca and Fe, Ca and Mn, Ca and Pb, Ca and As, Fe and Mn, Fe and Zn, Mn and As of A. dahurica were very significant. The correlation between Ca and Zn, Fe and Pb, Fe and As, Mn and Pb, Pb and Zn, Pb and Cu content were all significant in A. dahurica. This showed that the absorption of Ca, Fe, Mn, Pb, As, Mn, Zn and most of the inorganic elements had good synergy.
     6.Ten different A. dahurcia screened strains were cultivated in the same field in Yaan. The results showed that all traits of this 10 strains had significant difference. The root lengths ranged from 15.38 to 29.36 cm,22.04 cm in average. Circumferences ranged from 2.10 to 3.94 cm, average 3.16 cm. Yields per plot ranged from 4.7 to 9.7 kg,7.3 kg in average. The contents of imperatorin ranged from 0.997 to 2.516 mg/g,1.739 mg/g in average. And isoimperatorin contents ranged from 0.473 to 1.036 mg/g,0.736 mg/g in average. The results also showed that all traits of this 10 different A. dahurica strains grew in the same field in Suining were also significant different. The root lengths ranged from 21.77 to 33.29 cm,26.23 cm in average. Circumferences ranged from 2.94 to 4.37 cm, with an average of 3.57 cm. Yields per plot ranged from 17.0 to 23.5 kg,20.40 kg in average. The contents of imperatorin ranged from 1.671 to 2.721 mg/g,20.250 mg/g in average. And isoimperatorin contents ranged from 0.644 to 1.458 mg/g,1.075 mg/g in average. It indicated that distinct A. dahurica var. formosana lines could also be attained through extensive selection.
     7.The main agronomic traits and the main effective constituents of 10 different strains of A. dahurica grew in Yaan and Suining were compared. It is easy to find that, the root of A. dahurica strain grew in Suining were longer than the same strain grew in Yaan,4.19 cm longer in average. The root of A. dahurica strain grew in Suining were thicker than the same strain grew in Yaan,0.41 cm thicker in average. The yield per plot of A. dahurica strain grew in Suining were higher than those grew in Yaan, increased 13.1 kg in average. The average of yield per plot of A. dahurica strains grew in Suining was 2.79 times as that in Yaan. And the imperatorin and isoimperatorin contents of 10 different strains of A. dahurica grew in Suining were higher than Yaan,0.521mg/g and 0.339 mg/g in average, respectively. This further showed that the environment factors had important effects on A. dahurica indeed, and A. dahurica could had higher yield and best quality under the environment of Suining.
引文
[1]肖培根,李大鹏,杨世林,等.新编中药志一部[M].化学工业出版社.2002:337-343
    [2]王梦月,贾敏如.白芷本草考证[J].中药材,2004,27(5):382-385
    [3]国家药典委员会.中华人民共和国药典(一部)[S].北京,中国医药科技出版社,2010:97
    [4]李宏宇,戴跃进,张海波,等.中药川白芷的药理研究[J].华西药学杂志,1991,6(1):16-18
    [5]夏黎明,姚成.中药白芷的研究现状[J].中医药研究,2002,18(5):56-58
    [6]黄璐琦,王敏,付桂芳,等.中药白芷种质资源地RAPD分析[J].中国中药杂志.1999,24(8):457-459
    [7]潘泽惠,秦惠贞,吴竹君,等.白芷的核型研究及其意义[J].植物分类学报.1985,23(2):185-187
    [8]马逾英,熊英,贾敏如,等.川白芷种质的RAPD遗传标记研究[J].世界科学技术-中医药现代化.2004,6(02):76-80
    [9]王年鹤,黄璐琦,杨滨,等.白芷的基原植物、栽培历史以及其近缘野生植物演化的讨论.中国中药杂志[J].2001,26(10):733-736
    [10]杨滨,王敏,曹春,等.中药的分子遗传及其原植物分析[J].中国中药杂志,2004,39(9):654-657.
    [11]侯凯,吴卫,郑有良,等.川产白芷遗传多样性的ISSR分析[J].四川农业大学学报,2008,26(3):237-240
    [12]凤良元,鄢顺琴,杨瑞琴,等.五种不同产地的白芷药理作用的比较研究[J].安徽中医院学报,1990,9(2):56
    [13]李宏宇,戴跃进.不同商品白芷的药理研究[J].中国中药杂志,1991,16(9):560-562
    [14]野口敬身.河南实.白芷の成分研究(其一).伞形科植物の有效成分研究.(第五报)[J].药学杂志,1938,58(4):370
    [15]王梦月,贾敏如.白芷的化学成分研究进展[J].中药材,2002,25(6):446-449
    [16]陈贤春,王玉蓉,路世鹏.白芷提取工艺的研究[J].中成药,2005,27(2):145-147
    [17]梁明金,杨德广,贺浪冲.白芷中欧前胡素的提取方法研究[J].中成药,2000,22(12):829-831
    [18]刘红梅,张明贤.白芷中香豆素类成分的超临界流体萃取和GC-MS分析[J].中国中药杂志,2004,29(3):241-244
    [19]梁波,徐丽珍,邹忠梅,等.川白芷化学成分研究[J].中草药,2005,36(8):1132-1135
    [20]王婷婷,李丹毅,李清,等.正交试验法优选白芷的提取工艺[J].沈阳药科大学学报,2007,24(5):310-314
    [21]Kimura Y.. Histamine-Release Effectors from Angelica dahurica var. dahurica Root[J]. J, Nal, Prod,1997,60:249-251
    [22]Woo W. S., Lee,C. K.,etal.Isolation of drug metabolism modifiers from roots of Angelica Koreana.PlantaMediea,1982,45 (4):234-236
    [23]藤原英俊,横井利夫,谷升平,等.白芷成分研究(第一报)[J].药学杂志,1980,100(12):1258
    [24]周继铭,余朝菁,沈振东,等.白芷治疗银屑病有效成分研究[J].中成药研究,1980,(4):33
    [25]周继铭,余朝菁,杭宜卿.白芷的研究V.化学成分研究[J].中草药,1987,8(16):242-246
    [26]孙文基,赵晓文,薛利华.川白芷饮片中几种主要成分的分离与鉴定[J].药物分析杂志,1996,16(4):259-260
    [27]丁云梅,张涵庆.杭白芷香豆素的薄层分离和紫外分光光度法测定[J].药学通报,1981,16(8):16-17
    [28]张丽英,任艳春,张昭庆,等.白芷中欧前胡素的含量测定[J].中医药信息,2002,19(4):24
    [29]李宏宇,戴跃进,谢成科,等.川白芷中香豆素类成分的反相高效液相色谱分析[J].华西药学杂志,1990,5(4):231-233
    [30]王梦月,贾敏如,马逾英,等.不同入药部分及不同加工方法对白芷香豆素类成分含量的影响[J].中药材,2004,27(11):826-828
    [31]张志梅,翟志席,郭玉海,等.白芷干物质积累和异欧前胡素的动态研究[J].中草药,2005,36(6):902-904
    [32]高颖,马逾英,钟世红,等.川白芷中欧前胡素含量测定方法的比较[J].成都中医药大学学报,2006,29(4):49-51
    [33]韩娟.薄层扫描法测定中药白芷中香豆素类化合物含量的研究[J].中草药.1986,17(8):13
    [34]周钢,李革,刘晓方,等.高效液相色谱法对白芷中的欧前胡素含量测定的研究[J].中医药学刊,2003,21(2):320
    [35]王健.中药中香豆素的测定法,薄层扫描法测定白芷中异欧前胡素的含量[J].药物分析杂志,1988,8(2):87
    [36]王立人,李宏宇,谢成科.白芷中香豆精类成分的反相高效液相色谱测定[J].药学学报.1990,25(2):131-136
    [37]贾敏如,王梦月,金曲摸,等.HPLC测定13种白芷中欧前胡素和异欧前胡素的含量[J].华西药学杂志,2003,18(5):361-362
    [38]李宏宇,戴跃进.不同商品白芷中香豆素的薄层扫描法测定含量[J].华西药学杂志,1990,5(3):165-167
    [39]张志梅,翟志席,杨重军,等.白芷体内欧前胡素和异欧前胡素含量研究[J].中国中药杂志,2005,30(17):1377-1378
    [40]彭菲,张胜,刘塔斯,等.四倍体白芷药材中欧前胡素的含量测定[J].中国中药杂志,2002,27(6):426-427
    [41]中草药学,1979
    [42]马逾英,王娜,张利,等.亳白芷挥发油成分的气相色谱-质谱联用分析[J].时珍国医国药,2009,20(11):2667-2669
    [43]马逾英,王娜.不同前处理方法所得白芷挥发油成分的GC-MS分析[J].2009,32(4):50-53
    [44]弥宏,曲莉莉,任玉林.超临界萃取中药白芷的化学成分的气相色谱-质谱分析[J].2005,33(3):366-370
    [45]张国彬,薛敦渊,李兆林,等.杭白芷精油化学成分的研究[J].兰州大学学报(自科版),1989,25(3):159-160
    [46]乔善义,姚新生,刘传华.野生白芷挥发油成分的研究[J].中国药物化学杂志,1997,7(3):222
    [47]黄远征,徐成基,马最瑶.川白芷挥发油化学成分的研究[J].四川日化,1989,1:16-18
    [48]姚川,周成明,崔国印,等.白芷挥发油成分的研究[J].中药材,1990,13(12):34
    [49]李宏宇,戴跃进,谢成科.川白芷的挥发油成分分析[J].华西药学杂志,1990,5(2):79-82
    [50]胡孝丰,张瑞光.不同产地川牛膝、川白芷横切面各结构区域无机成分分布特点[J].中药材,1994,17(12):25
    [51]戴跃进,谢成科,李宏宇.白芷中的微量元素分析[J].华西医药杂志,1990,5(1):22-23
    [52]魏久宁.四川七种地道药材中微量元素的分析研究[J].华西医大学报,2001,32(4):634-635
    [53]陈兴福,丁德蓉,刘岁荣,等.白芷生长土壤的研究[J].中国中药杂志,1994,19(10):591-593
    [54]陈兴福,丁德蓉,刘岁荣,等.白芷生态环境和土壤理化特性的研究[J].中草药,1996,27(8):489-491
    [55]张志梅.白芷质量性状形成及栽培调控研究[学位论文].北京,中国农业大学,2005
    [56]陈兴福,卢进.播种期对白芷早期抽薹影响的研究[J].中国中药杂志,1999,24(4):211-212
    [57]丁德容,卢进,陈兴福,等.肥料种类对白芷早期抽薹与产量的影响研究[J].中国中药杂志,1999,24(1):23-24
    [58]丁德容,卢进,陈兴福,等.施肥措施对白芷早期抽薹与产量的影响研究[J].中草药,1999,30(2):135-137
    [59]杨富荣,杨海莹,郭敦志,等.白芷早薹及防止方法初探[J].中药材,2001,24(10):708
    [60]彭菲,郭显.川白芷离体培养与多倍体诱导过程中的形态组织学观察[J].中国中药杂志,2000,25(1):17-19
    [61]彭菲,王凤翱.川白芷试管苗再生的研究[J].中草药,1997,28(6):364-366
    [62]彭菲,张胜,刘塔斯,等.四倍体白芷药材中欧前胡素的含量测定[J].中国中药杂志,2002,27(6):426-427
    [63]钟世红,马逾英,贾敏如,等.川白芷HPLC指纹图谱研究[J].世界科学技术-中医药现代化,7(6):67-71
    [64]张玉芳,余红梅.硫熏对白芷香豆素类成分含量的影响研究[J].中国中药杂志,1997,22(9):536-538
    [65]赵恒,周斌,孔英华,等.产地加工方法对白芷化学成分的影响[J].中药材,1900,19(3):134-136
    [66]戴跃进,李宏宇,谢成科.川白芷采收加工的质量研究[J].华西药学杂志,1990,5(4):241-242
    [67]邓捷圆,高广慧,赵春杰.HPLC法同时测定不同产地白芷中2种香豆素的含量[J].沈阳药科大学学报.2004,21(5):354-357
    [68]陈翠,袁理春,杨丽英,等.不同海拔、土壤类型及肥力对云南重楼产量和质量的影响[J].西南农业学报,2009,22(5):1388-1390
    [69]郭衍银.生姜产量与土壤特性关系研究[A].中国环境科学学会2006年学术年会优秀论文集(下卷)
    [70]王建安,徐增莱,吴国荣,等.氮磷钾对盾叶薯蓣产量及薯蓣皂苷元含量的影响[J].中药材,2004,27(12):891-893
    [71]袁勇,黄慧莲,刘贤旺.无机肥料对中药有效成分含量的影响.江西林业科技,2000,1:29-30
    [72]徐桂新,李向民,乔振杰.土壤水分对黄姜根茎产量和皂素含量的影响[J].西北农业学报,2006,15(6):163-]65
    [73]漆小雪,李锋,李光照,等.罗汉果产区土壤肥力状况与罗汉果产量和品质的关系[J].广西科学,2004,11(3):273-277
    [74]素凤梅,陈士林,任德全.道地药材产地适宜性研究[J].中国中药杂志,2005,30(19):1485-1488
    [75]Bouwmeester H.A.,Davies A.R.,Smid H.G., et al.Physiological limitations to carvone yield in caraway(Carumcarvi L.).Ind.Crops Prod,1995,4:39-51
    [76]Herminio B.,Antonio B..Environmental factors affecting chemical variability of essential oi Is in Thumus piperella L.Biochem.Syst. and Ecol,1998,26:811-822
    [77]Jens R.,Ruth M.,Steinar D.. Chemotypical variation of Tansy (tanacetum vulgara L.) from 40 different locations in Norway.J.Agric.Food Chem,2004,52,1742-1748
    [78]Park H.,Lee M.K..Relationship between ginsenosides and mineral contents in Panax ginse ng grown with nutrient solution. Hanguk Nonghwa Hakhoechi,1987,30(2):186-191
    [79]王文杰,张京教,赵长琦.环境条件对伊贝母生物碱含量的影响[J].中药材,1989,12(2):3-5
    [80]郭巧生,梁迎暖,张重义,等.土壤因子对怀菊质量影响研究[J].中国中药杂志,2008,33(2):123-128
    [81]张永清,李岩坤.影响药用植物体内生物碱含量的因素.齐鲁中医药情报,1992,3:10-12
    [82]张康健,王亚琴,马希汉,等.杜仲叶次生代谢物生态学研究初报[J].林业科学,1999,35(6)28-35
    [83]王亚琴.杜仲叶有效成分的地理学研究[J].广东药学院学报,2000,16(3):173-176
    [84]黄泰康.天然药用植物与土壤环境的药效学研究[M].长春:吉林人民出版社,1994,68
    [85]吴炳辅,崔万钧.中药中微量元素研究的现状和展望[J].中草药,1986,17(4):38-42
    [86]范俊安,易平.四川道地药材与微量元素相关性初步研究.中药材,1991,14(1):3-5
    [87]郭兰萍,黄璐琦,阎玉凝.土壤中无机元素对茅苍术道地性的影响.中国中药杂志,2002,27(4):245-250
    [88]汪丽娅,孟繁蕴,张文生,等.黄草乌原药材与土壤无机元素相关性研究.北京中医药大学学报,2005,28(3):68-71
    [89]中国国家标准汇编[M].2002
    [90]中华人民共和国国家标准[M].1994
    [91]鲁如坤.土壤农业化学分析方法[M].北京,中国农业科技出版社,2000
    [92]唐启义,冯明光.实用统计分析及其DPS数据处理系统[M].北京,科学出版社,2002:43-55,294-311
    [93]陈伟忠.中药微量元素的研究概况[J].右江医学,1991,19(1):31-33
    [94]胡育筑,郭环娟,王志群,等.中药四性和微量元素含量关系的初步研究[J].1992,23(6):348-353

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700