新城疫病毒NDV4-C株反向遗传操作系统的建立及耐热应用研究
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摘要
新城疫(Newcastle disease, ND)是由新城疫病毒(Newcastle disease virus, NDV)引起的急性、高度接触性的禽类传染病，给世界养禽业造成极大的经济损失。目前，疫苗接种仍然是预防和控制该病普遍采用的方法。
NDV4是广泛应用于东南亚和非洲等热带亚热带地区的一种耐热疫苗株，具有耐热性和免疫持续期长等优点。NDV4-C株是V4疫苗株在SPF鸡胚上连续传代筛选获得的一株耐热无毒株，目前NDV4毒株的研究相对滞后，一个主要的原因是反向遗传操作系统平台尚未建立，相应的耐热性和免疫原性研究也鲜有报道。为此，本研究以NDV4-C株为研究对象，在对NDV4-C株全基因组序列系统解析的基础上，首次建立了分别以T7启动子和CMV启动子的NDV4-C株反向遗传操作系统，拯救病毒rNDV4-C (T7)和rNDV4-C (CMV)的耐热性和生物学特性均与亲本毒株相似。同时，为了评估NDV4-C株作为载体的可行性，构建了表达增强型绿色荧光蛋白的重组新城疫病毒rNDV4-eGFP，其在SPF鸡胚上生长特性与亲本病毒相似，重组病毒能够稳定表达增强型绿色荧光蛋白至少15代，结果表明，rNDV4-C具有作为病毒载体应用的潜力。
聚合酶蛋白对副粘病毒的耐热性作用已有报道，为了调查聚合酶蛋白对NDV4-C株耐热性的作用影响，我们分别构建了以耐热株NDV4-C和不耐热株LaSota为骨架替换聚合酶基因的嵌合病毒株rNDV4-La-L和rLaSota-V4-L，测定其耐热性和病毒生物学特性，结果表明以NDV4-C为骨架的嵌合病毒耐热性降低，生长滴度低于NDV4-C株的生长滴度。反之，以LaSota为骨架嵌合NDV4-C株聚合酶基因的嵌合病毒耐热性有一定程度的提高，生长滴度也较LaSota的滴度升高。此外，用表达荧光素酶的微基因组系统检测聚合酶蛋白活性，结果表明NDV4-C株的聚合酶蛋白具有更高的活性。因此聚合酶蛋白影响了NDV4-C的耐热性。
当前的NDV疫苗免疫可有效控制新城疫疫情，但不能够阻止新城疫病毒的排毒、传播和感染。V4疫苗株与LaSota、Clone30和Hitchner B1等疫苗株相比，免疫诱导的体液免疫抗体水平低，但粘膜免疫水平较高。为了探讨能否在保持NDV4-C耐热性的基础上提高其免疫应答水平，在前两项工作的基础上，我们构建并拯救了以NDV4-C为骨架嵌合当前流行的基因Ⅶ型强毒株Go/CH/HLJ/LL01/08(LL01/08)囊膜蛋白的两株嵌合病毒rNDV4-HNLL01和rNDV4-MuF/HNLL01。相对于亲本病毒LL01/08，嵌合病毒保持了低毒力，生长略有延迟，耐热性略有下降但保持了耐热性。病毒株rNDV4-C、rNDV4-HNLL01、rNDV4-MuF/HNLL01和疫苗株LaSota以1×10~6EID_(50)剂量滴鼻免疫两周龄SPF鸡，能够诱导不同程度的体液免疫和粘膜免疫水平，免疫两周后以1×10~6EID_(50)的剂量强毒株LL01/08攻毒。动物实验结果表明，嵌合病毒诱导产生的IgG抗体水平并没有升高，但在血清和十二指肠产生的IgA抗体水平较亲本病毒rNDV4-C显著提高。嵌合病毒能够完全保护SPF鸡抵抗致死剂量强毒的攻击，更重要地是嵌合病毒rNDV4-MuF/HNLL01免疫组的排毒量较NDV4-C免疫组有显著的下降。结果表明嵌合病毒rNDV4-MuF/HNLL01能够作为防控当前流行NDV的候选疫苗株。
Newcastle disease (ND), caused by Newcastle disease virus (NDV), is an acute and highlycontagious disease which affects many domestic and wild avian species and causes severe economiclosses to the poultry industry in the world. Currently, vaccination remains a primary strategy for NDcontrol.
NDV4vaccine strain is being widely used in the tropical and sub-tropical countries of SoutheastAsia and Africa due to its thermostability and long-lasting immunity. NDV4-C is an avirulent andthermostable strain which is screened by passaging in specific-pathogen-free (SPF) chicken eggs. Todate, the reverse genetics of NDV4strain is unavailable, which accounts for the limited research ofNDV4strain. Based on the systemic sequence analysis of NDV4-C, in this study, we established thereverse genetics system for the avirulent and thermostable NDV4-C strain. Successful recovery ofNDV4-C was achieved by using either T7RNA polymerase or cellular RNA polymerase II to drivetranscription of the full-length virus antigenome from cloned cDNA. The recovered viruses rNDV4-C(T7) and rNDV4-C (CMV) showed similar thermostability and biological properties as the parentalstrain NDV4-C. The potential of rNDV4-C (T7) to serve as a viral vector was assessed by generating arecombinant virus, rNDV4-eGFP, which expressed enhanced green fluorescent protein (eGFP). TherNDV4-eGFP could stably carry and express eGFP for at least fifteen passages. These results indicatedthat the rNDV4-C (T7) has the potential to serve as a live vaccine vector.
It is reported that the large protein is associated with the temperature-sensitive in theparamyxovirus. Here, we engineered these chimeras rNDV4-La-L and rLaSota-V4-L by exchanging theL gene between the thermostable NDV4-C strain and the thermolabile LaSota strain to evaluate the roleof large protein in NDV4-C thermostability. Thermostability of chimeric viruses was examined bydetermining the infectious titre after incubation for different periods of time at55°C. The characteristicof chimeric viruses was also evaluated in embryonated SPF chickens and eggs. Chimera rNDV4-La-Lposses decreased thermostability and lower viral titre, and rather than vise versa, suggesting that the Lgene contributes to the thermostability of NDV4-C. Furthermore, the in vitro replication assay with aminigenome system expressing luciferase showed that the L activity of NDV4-C was higher than that ofLaSota, which contributes to the thermostability of NDV.
Routine and/or intensive vaccinations have been shown to be effective in preventing disease andmortality but not in preventing viral shedding, transmission, and infection, NDV4vaccine inducedlower humoral immune response antibody level than the LaSota, Clone30, and Hitchner B1, but couldinduce good mucosal immune response. We engineered two chimeras, rNDV4-HNLL01andrNDV4-MuF/HNLL01, which combined the thermostability of the NDV4-C strain with the goodimmunogenicity of the velogenic sub-genotype VIId Go/CH/HLJ/LL01/08(LL01/08) strain.Thermostability of these chimeras was confirmed by determining the infectious titre after incubation for different periods of time at55°C. Both chimeras fulfilled the criteria for lentogenic virus.Two-week-old SPF chickens, which were inoculated by the oculonasal route with each of thesechimeras, the rNDV4-C strain, or the widely used NDV vaccine strain LaSota, respectively, produceddifferent levels of humoral IgG and mucosal IgA antibodies. The IgG antibody level induced by LaSotawas higher than that induced by the other groups, while these chimeras induced higher levels of IgAantibody than that of rNDV4-C in sera and dodecadactylon. The animal experiments demonstrated thatall vaccinated groups were fully protected from morbidity and mortality. Importantly, bothrNDV4-MuF/HNLL01-and LaSota-vaccinated chickens showed a significant reduction in viralshedding in the cloaca compared to chickens of the other vaccinated groups. In summary, our datasuggest that the lentogenic and thermostable rNDV4-MuF/HNLL01strain, which induces enhancedmucosal immune response, could be a promising vaccine candidate for the control of current NDepidemics.
NDV4是广泛应用于东南亚和非洲等热带亚热带地区的一种耐热疫苗株，具有耐热性和免疫持续期长等优点。NDV4-C株是V4疫苗株在SPF鸡胚上连续传代筛选获得的一株耐热无毒株，目前NDV4毒株的研究相对滞后，一个主要的原因是反向遗传操作系统平台尚未建立，相应的耐热性和免疫原性研究也鲜有报道。为此，本研究以NDV4-C株为研究对象，在对NDV4-C株全基因组序列系统解析的基础上，首次建立了分别以T7启动子和CMV启动子的NDV4-C株反向遗传操作系统，拯救病毒rNDV4-C (T7)和rNDV4-C (CMV)的耐热性和生物学特性均与亲本毒株相似。同时，为了评估NDV4-C株作为载体的可行性，构建了表达增强型绿色荧光蛋白的重组新城疫病毒rNDV4-eGFP，其在SPF鸡胚上生长特性与亲本病毒相似，重组病毒能够稳定表达增强型绿色荧光蛋白至少15代，结果表明，rNDV4-C具有作为病毒载体应用的潜力。
聚合酶蛋白对副粘病毒的耐热性作用已有报道，为了调查聚合酶蛋白对NDV4-C株耐热性的作用影响，我们分别构建了以耐热株NDV4-C和不耐热株LaSota为骨架替换聚合酶基因的嵌合病毒株rNDV4-La-L和rLaSota-V4-L，测定其耐热性和病毒生物学特性，结果表明以NDV4-C为骨架的嵌合病毒耐热性降低，生长滴度低于NDV4-C株的生长滴度。反之，以LaSota为骨架嵌合NDV4-C株聚合酶基因的嵌合病毒耐热性有一定程度的提高，生长滴度也较LaSota的滴度升高。此外，用表达荧光素酶的微基因组系统检测聚合酶蛋白活性，结果表明NDV4-C株的聚合酶蛋白具有更高的活性。因此聚合酶蛋白影响了NDV4-C的耐热性。
当前的NDV疫苗免疫可有效控制新城疫疫情，但不能够阻止新城疫病毒的排毒、传播和感染。V4疫苗株与LaSota、Clone30和Hitchner B1等疫苗株相比，免疫诱导的体液免疫抗体水平低，但粘膜免疫水平较高。为了探讨能否在保持NDV4-C耐热性的基础上提高其免疫应答水平，在前两项工作的基础上，我们构建并拯救了以NDV4-C为骨架嵌合当前流行的基因Ⅶ型强毒株Go/CH/HLJ/LL01/08(LL01/08)囊膜蛋白的两株嵌合病毒rNDV4-HNLL01和rNDV4-MuF/HNLL01。相对于亲本病毒LL01/08，嵌合病毒保持了低毒力，生长略有延迟，耐热性略有下降但保持了耐热性。病毒株rNDV4-C、rNDV4-HNLL01、rNDV4-MuF/HNLL01和疫苗株LaSota以1×10~6EID_(50)剂量滴鼻免疫两周龄SPF鸡，能够诱导不同程度的体液免疫和粘膜免疫水平，免疫两周后以1×10~6EID_(50)的剂量强毒株LL01/08攻毒。动物实验结果表明，嵌合病毒诱导产生的IgG抗体水平并没有升高，但在血清和十二指肠产生的IgA抗体水平较亲本病毒rNDV4-C显著提高。嵌合病毒能够完全保护SPF鸡抵抗致死剂量强毒的攻击，更重要地是嵌合病毒rNDV4-MuF/HNLL01免疫组的排毒量较NDV4-C免疫组有显著的下降。结果表明嵌合病毒rNDV4-MuF/HNLL01能够作为防控当前流行NDV的候选疫苗株。
Newcastle disease (ND), caused by Newcastle disease virus (NDV), is an acute and highlycontagious disease which affects many domestic and wild avian species and causes severe economiclosses to the poultry industry in the world. Currently, vaccination remains a primary strategy for NDcontrol.
NDV4vaccine strain is being widely used in the tropical and sub-tropical countries of SoutheastAsia and Africa due to its thermostability and long-lasting immunity. NDV4-C is an avirulent andthermostable strain which is screened by passaging in specific-pathogen-free (SPF) chicken eggs. Todate, the reverse genetics of NDV4strain is unavailable, which accounts for the limited research ofNDV4strain. Based on the systemic sequence analysis of NDV4-C, in this study, we established thereverse genetics system for the avirulent and thermostable NDV4-C strain. Successful recovery ofNDV4-C was achieved by using either T7RNA polymerase or cellular RNA polymerase II to drivetranscription of the full-length virus antigenome from cloned cDNA. The recovered viruses rNDV4-C(T7) and rNDV4-C (CMV) showed similar thermostability and biological properties as the parentalstrain NDV4-C. The potential of rNDV4-C (T7) to serve as a viral vector was assessed by generating arecombinant virus, rNDV4-eGFP, which expressed enhanced green fluorescent protein (eGFP). TherNDV4-eGFP could stably carry and express eGFP for at least fifteen passages. These results indicatedthat the rNDV4-C (T7) has the potential to serve as a live vaccine vector.
It is reported that the large protein is associated with the temperature-sensitive in theparamyxovirus. Here, we engineered these chimeras rNDV4-La-L and rLaSota-V4-L by exchanging theL gene between the thermostable NDV4-C strain and the thermolabile LaSota strain to evaluate the roleof large protein in NDV4-C thermostability. Thermostability of chimeric viruses was examined bydetermining the infectious titre after incubation for different periods of time at55°C. The characteristicof chimeric viruses was also evaluated in embryonated SPF chickens and eggs. Chimera rNDV4-La-Lposses decreased thermostability and lower viral titre, and rather than vise versa, suggesting that the Lgene contributes to the thermostability of NDV4-C. Furthermore, the in vitro replication assay with aminigenome system expressing luciferase showed that the L activity of NDV4-C was higher than that ofLaSota, which contributes to the thermostability of NDV.
Routine and/or intensive vaccinations have been shown to be effective in preventing disease andmortality but not in preventing viral shedding, transmission, and infection, NDV4vaccine inducedlower humoral immune response antibody level than the LaSota, Clone30, and Hitchner B1, but couldinduce good mucosal immune response. We engineered two chimeras, rNDV4-HNLL01andrNDV4-MuF/HNLL01, which combined the thermostability of the NDV4-C strain with the goodimmunogenicity of the velogenic sub-genotype VIId Go/CH/HLJ/LL01/08(LL01/08) strain.Thermostability of these chimeras was confirmed by determining the infectious titre after incubation for different periods of time at55°C. Both chimeras fulfilled the criteria for lentogenic virus.Two-week-old SPF chickens, which were inoculated by the oculonasal route with each of thesechimeras, the rNDV4-C strain, or the widely used NDV vaccine strain LaSota, respectively, produceddifferent levels of humoral IgG and mucosal IgA antibodies. The IgG antibody level induced by LaSotawas higher than that induced by the other groups, while these chimeras induced higher levels of IgAantibody than that of rNDV4-C in sera and dodecadactylon. The animal experiments demonstrated thatall vaccinated groups were fully protected from morbidity and mortality. Importantly, bothrNDV4-MuF/HNLL01-and LaSota-vaccinated chickens showed a significant reduction in viralshedding in the cloaca compared to chickens of the other vaccinated groups. In summary, our datasuggest that the lentogenic and thermostable rNDV4-MuF/HNLL01strain, which induces enhancedmucosal immune response, could be a promising vaccine candidate for the control of current NDepidemics.
引文
1.曹殿军，孙建宏，闫丽辉，等。新城疫LaSota和V4疫苗免疫鸡和免疫攻毒鸡的特异性IgA抗体动态变化规律比较[J].中国预防兽医学报,2001,23(2):84-86.
2.姜延龙.新城疫病毒V4株全基因组测序及全长cDNA克隆的构建.[姜延龙博士学位论文].黑龙江：东北农业大学,2009.
3.黎秋华,邵华斌，杨峻.鸡新城疫病毒HB92株生物学特性研究(II)—对热的稳定性及在鸡群中的自然传播性试验[J].湖北畜牧兽医,1996,2:1-2.
4.殷震,刘景华(主编).(1997).动物病毒学[M].北京:科学出版社.
5.邵华斌,黎秋华,杨峻.鸡新城疫病毒HB92株生物学特性研究(I)—对鸽、鹌鹑的免疫试验[J].湖北畜牧兽医,1996,1:4-5
6.吴宝成,周金法,孙莉.新城疫V4+C30复合疫苗的制备和免疫研究[J].中国预防兽医学报，2001,23(6):451-454.
7.徐振东,孙莉,周金法.新城疫V4株弱毒变种的选育[J].中国畜禽传染病,1996,6:20-23.
8. Abolnik, C., Horner, R.F., Bisschop, S.P., Parker, M.E., Romito, M., Viljoen, G.J.,2004. Aphylogenetic study of South African Newcastle disease virus strains isolated between1990and2002suggests epidemiological origins in the Far East. Arch Virol149,603-619.
9. Al-Garib, S.O., Gielkens, A.L., Gruys, D.E., Hartog, L., Koch, G.,2003. Immunoglobulin classdistribution of systemic and mucosal antibody responses to Newcastle disease in chickens. AvianDis47,32-40.
10. Alexander, D.,2000. Newcastle disease and other avian paramyxoviruses. Revue Scientifique etTechnique-Office International des Epizooties19,443-455.
11. Altomonte, J., Marozin, S., Schmid, R.M., Ebert, O.,2010. Engineered newcastle disease virus asan improved oncolytic agent against hepatocellular carcinoma. Mol Ther18,275-284.
12. Bailey, D., Chard, L.S., Dash, P., Barrett, T., Banyard, A.C.,2007. Reverse genetics forpeste-des-petits-ruminants virus (PPRV): promoter and protein specificities. Virus Res126,250-255.
13. Baron, M.D., Foster-Cuevas, M., Baron, J., Barrett, T.,1999. Expression in cattle of epitopes of aheterologous virus using a recombinant rinderpest virus. J Gen Virol80(Pt8),2031-2039.
14. Berinstein, A., Vazquez-Rovere, C., Asurmendi, S., Gomez, E., Zanetti, F., Zabal, O., Tozzini, A.,Conte Grand, D., Taboga, O., Calamante, G., Barrios, H., Hopp, E., Carrillo, E.,2005. Mucosal andsystemic immunization elicited by Newcastle disease virus (NDV) transgenic plants as antigens.Vaccine23,5583-5589.
15. Blakqori, G., Kochs, G., Haller, O., Weber, F.,2003. Functional L polymerase of La Crosse virusallows in vivo reconstitution of recombinant nucleocapsids. J Gen Virol84,1207-1214.
16. Blumberg, B.M., Kolakofsky, D.,1981. Intracellular vesicular stomatitis virus leader RNAs arefound in nucleocapsid structures. J Virol40,568-576.
17. Britton, P., Green, P., Kottier, S., Mawditt, K.L., Penzes, Z., Cavanagh, D., Skinner, M.A.,1996.Expression of bacteriophage T7RNA polymerase in avian and mammalian cells by a recombinantfowlpox virus. J Gen Virol77(Pt5),963-967.
18. Brown, D.D., Collins, F.M., Duprex, W.P., Baron, M.D., Barrett, T., Rima, B.K.,2005.'Rescue' ofmini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins. J GenVirol86,1077-1081.
19. Brown, P.A., Lupini, C., Catelli, E., Clubbe, J., Ricchizzi, E., Naylor, C.J.,2011. A singlepolymerase (L) mutation in avian metapneumovirus increased virulence and partially maintainedvirus viability at an elevated temperature. J Gen Virol92,346-354.
20. Buchholz, U.J., Finke, S., Conzelmann, K.K.,1999. Generation of bovine respiratory syncytialvirus (BRSV) from cDNA: BRSV NS2is not essential for virus replication in tissue culture, andthe human RSV leader region acts as a functional BRSV genome promoter. J Virol73,251-259.
21. Buckland, R., and F.Wild.,1989. Leucine zipper motif extends. Nature,338:547.
22. Bukreyev, A., Camargo, E., Collins, P.L.,1996. Recovery of infectious respiratory syncytial virusexpressing an additional, foreign gene. J Virol70,6634-6641.
23. Bukreyev, A., Huang, Z., Yang, L., Elankumaran, S., St Claire, M., Murphy, B.R., Samal, S.K.,Collins, P.L.,2005. Recombinant newcastle disease virus expressing a foreign viral antigen isattenuated and highly immunogenic in primates. J Virol79,13275-13284.
24. Carr, C.M., Chaudhry, C., Kim, P.S.,1997. Influenza hemagglutinin is spring-loaded by ametastable native conformation. Proc Natl Acad Sci U S A94,14306-14313.
25. Cattaneo, R., Rebmann, G., Schmid, A., Baczko, K., ter Meulen, V., Billeter, M.A.,1987. Alteredtranscription of a defective measles virus genome derived from a diseased human brain. EMBO J6,681-688.
26. Chambers, P., Millar, N.S., Bingham, R.W., Emmerson, P.T.,1986. Molecular cloning ofcomplementary DNA to Newcastle disease virus, and nucleotide sequence analysis of the junctionbetween the genes encoding the haemagglutinin-neuraminidase and the large protein. J Gen Virol67(Pt3),475-486.
27. Chambers, P., Pringle, C.R., Easton, A.J.,1990. Heptad repeat sequences are located adjacent tohydrophobic regions in several types of virus fusion glycoproteins. J Gen Virol71(Pt12),3075-3080.
28. Chen, F., Liu, J., Liu, D., Yan, Z., Ji, J., Qin, J., Li, H., Ma, J., Bi, Y., Xie, Q.,2012. CompleteGenome Sequence of a Newcastle Disease Virus Strain Isolated from Broiler Breeder Flocks inChina. J Virol86,12461-12462.
29. Chen, L., Gorman, J.J., McKimm-Breschkin, J., Lawrence, L.J., Tulloch, P.A., Smith, B.J., Colman,P.M., Lawrence, M.C.,2001. The structure of the fusion glycoprotein of Newcastle disease virussuggests a novel paradigm for the molecular mechanism of membrane fusion. Structure9,255-266.
30. Collins, M.S., Bashiruddin, J.B., Alexander, D.J.,1993. Deduced amino acid sequences at thefusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity andpathogenicity. Arch Virol128,363-370.
31. Courtney, S.C., Gomez, D., Susta, L., Hines, N., Pedersen, J.C., Miller, P.J., Afonso, C.L.,2012.Complete genome sequencing of a novel newcastle disease virus isolate circulating in layerchickens in the dominican republic. J Virol86,9550.
32. Crennell, S., Takimoto, T., Portner, A., Taylor, G.,2000. Crystal structure of the multifunctionalparamyxovirus hemagglutinin-neuraminidase. Nat Struct Biol7,1068-1074.
33. Curran, J., Boeck, R., Kolakofsky, D.,1991. The Sendai virus P gene expresses both an essentialprotein and an inhibitor of RNA synthesis by shuffling modules via mRNA editing. EMBO J10,3079-3085.
34. Curran, J., Marq, J.B., Kolakofsky, D.,1995. An N-terminal domain of the Sendai paramyxovirus Pprotein acts as a chaperone for the NP protein during the nascent chain assembly step of genomereplication. J Virol69,849-855.
35. Czegledi, A., Ujvari, D., Somogyi, E., Wehmann, E., Werner, O., Lomniczi, B.,2006. Thirdgenome size category of avian paramyxovirus serotype1(Newcastle disease virus) andevolutionary implications. Virus Res120,36-48.
36. de Leeuw, O.S., Koch, G., Hartog, L., Ravenshorst, N., Peeters, B.P.,2005. Virulence of Newcastledisease virus is determined by the cleavage site of the fusion protein and by both the stem regionand globular head of the haemagglutinin-neuraminidase protein. J Gen Virol86,1759-1769.
37. DiNapoli, J.M., Kotelkin, A., Yang, L., Elankumaran, S., Murphy, B.R., Samal, S.K., Collins, P.L.,Bukreyev, A.,2007. Newcastle disease virus, a host range-restricted virus, as a vaccine vector forintranasal immunization against emerging pathogens. Proc Natl Acad Sci U S A104,9788-9793.
38. DiNapoli, J.M., Nayak, B., Yang, L., Finneyfrock, B.W., Cook, A., Andersen, H., Torres-Velez, F.,Murphy, B.R., Samal, S.K., Collins, P.L., Bukreyev, A.,2010.
39. Foster, H.A., Chitukuro, H.R., Tuppa, E., Mwanjala, T., Kusila, C.,1999. Thermostable Newcastledisease vaccines in Tanzania. Vet Microbiol68,127-130.
40. Newcastle disease virus-vectored vaccines expressing the hemagglutinin or neuraminidase proteinof H5N1highly pathogenic avian influenza virus protect against virus challenge in monkeys. JVirol84,1489-1503.
41. DJ Cao, J., Sun, LH,Yan, PX, Liu et al. July15-20,2001. the changes of IgG, IgM and IgAantibody level of chickens immunized with NDV LaSota or V4vaccine strain. In: Proceedings ofthe Sixth International Veterinary Immunology Symposium, Uppsala, Sweden, p102.
42. Dortmans, J.C., Koch, G., Rottier, P.J., Peeters, B.P.,2009. Virulence of pigeon paramyxovirus type1does not always correlate with the cleavability of its fusion protein. J Gen Virol90,2746-2750.
43. Dortmans, J.C., Rottier, P.J., Koch, G., Peeters, B.P.,2010. The viral replication complex isassociated with the virulence of Newcastle disease virus. J Virol84,10113-10120.
44. Dortmans, J.C., Rottier, P.J., Koch, G., Peeters, B.P.,2011. Passaging of a Newcastle disease viruspigeon variant in chickens results in selection of viruses with mutations in the polymerase complexenhancing virus replication and virulence. J Gen Virol92,336-345.
45. Egelman, E.H., Wu, S.S., Amrein, M., Portner, A., Murti, G.,1989. The Sendai virus nucleocapsidexists in at least four different helical states. J Virol63,2233-2243.
46. Errington, W., Emmerson, P.T.,1997. Assembly of recombinant Newcastle disease virusnucleocapsid protein into nucleocapsid-like structures is inhibited by the phosphoprotein. J GenVirol78,2335-2339.
47. Errington, W., Steward, M., Emmerson, P.T.,1995. A diagnostic immunoassay for Newcastledisease virus based on the nucleocapsid protein expressed by a recombinant baculovirus. Journal ofvirological methods55,357-365.
48. Estevez, C., King, D., Seal, B., Yu, Q.,2007. Evaluation of Newcastle disease virus chimerasexpressing the Hemagglutinin-Neuraminidase protein of velogenic strains in the context of amesogenic recombinant virus backbone. Virus Res129,182-190.
49. Freiberg, A., Dolores, L.K., Enterlein, S., Flick, R.,2008. Establishment and characterization ofplasmid-driven minigenome rescue systems for Nipah virus: RNA polymerase I-and T7-catalyzedgeneration of functional paramyxoviral RNA. Virology370,33-44.
50. Garcin, D., Pelet, T., Calain, P., Roux, L., Curran, J., Kolakofsky, D.,1995. A highlyrecombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA:generation of a novel copy-back nondefective interfering virus. EMBO J14,6087-6094.
51. Ge, J., Deng, G., Wen, Z., Tian, G., Wang, Y., Shi, J., Wang, X., Li, Y., Hu, S., Jiang, Y., Yang, C.,Yu, K., Bu, Z., Chen, H.,2007. Newcastle disease virus-based live attenuated vaccine completelyprotects chickens and mice from lethal challenge of homologous and heterologous H5N1avianinfluenza viruses. J Virol81,150-158.
52. Groseth, A., Feldmann, H., Theriault, S., Mehmetoglu, G., Flick, R.,2005. RNA polymeraseI-driven minigenome system for Ebola viruses. J Virol79,4425-4433.
53. Haller, A.A., MacPhail, M., Mitiku, M., Tang, R.S.,2001. A single amino acid substitution in theviral polymerase creates a temperature-sensitive and attenuated recombinant bovine parainfluenzavirus type3. Virology288,342-350.
54. Hamaguchi, M., Yoshida, T., Nishikawa, K., Naruse, H., Nagai, Y.,1983. Transcriptive complex ofNewcastle disease virus. I. Both L and P proteins are required to constitute an active complex.Virology128,105-117.
55. He, B., Paterson, R.G., Ward, C.D., Lamb, R.A.,1997. Recovery of infectious SV5from clonedDNA and expression of a foreign gene. Virology237,249-260.
56. Hu, H., Roth, J.P., Estevez, C.N., Zsak, L., Liu, B., Yu, Q.,2011. Generation and evaluation of arecombinant Newcastle disease virus expressing the glycoprotein (G) of avian metapneumovirussubgroup C as a bivalent vaccine in turkeys. Vaccine29,8624-8633.
57. Hu, S., Ma, H., Wu, Y., Liu, W., Wang, X., Liu, Y., Liu, X.,2009. A vaccine candidate of attenuatedgenotype VII Newcastle disease virus generated by reverse genetics. Vaccine27,904-910.
58. Huang, Y., Wan, H.Q., Liu, H.Q., Wu, Y.T., Liu, X.F.,2004a. Genomic sequence of an isolate ofNewcastle disease virus isolated from an outbreak in geese: a novel six nucleotide insertion in thenon-coding region of the nucleoprotein gene. Brief Report. Arch Virol149,1445-1457.
59. Huang, Z., Elankumaran, S., Yunus, A.S., Samal, S.K.,2004b. A recombinant Newcastle diseasevirus (NDV) expressing VP2protein of infectious bursal disease virus (IBDV) protects againstNDV and IBDV. J Virol78,10054-10063.
60. Huang, Z., Krishnamurthy, S., Panda, A., Samal, S.K.,2003. Newcastle disease virus V protein isassociated with viral pathogenesis and functions as an alpha interferon antagonist. J Virol77,8676-8685.
61. Huang, Z., Panda, A., Elankumaran, S., Govindarajan, D., Rockemann, D.D., Samal, S.K.,2004c.The hemagglutinin-neuraminidase protein of Newcastle disease virus determines tropism andvirulence. J Virol78,4176-4184.
62. Ibrahim, A.L., Chulan, U., Babjee, A.M.,1981. An assessment of the Australian V4strain ofNewcastle disease virus as a vaccine by spray, aerosol and drinking water administration. Aust VetJ57,277-280.
63. Ideris, A., Ibrahim, A.L., Spradbrow, P.B.,1990. Vaccination of chickens against Newcastle diseasewith a food pellet vaccine. Avian Pathol19,371-384.
64. Inoue, K., Shoji, Y., Kurane, I., Iijima, T., Sakai, T., Morimoto, K.,2003. An improved method forrecovering rabies virus from cloned cDNA. J Virol Methods107,229-236.
65. Jayawardane, G.W., Spradbrow, P.B.,1995a. Cell-mediated immunity in chickens vaccinated withthe V4strain of Newcastle disease virus. Vet Microbiol46,37-41.
66. Jayawardane, G.W., Spradbrow, P.B.,1995b. Mucosal immunity in chickens vaccinated with theV4strain of Newcastle disease virus. Vet Microbiol46,69-77.
67. Jeon, W.J., Lee, E.K., Lee, Y.J., Jeong, O.M., Kim, Y.J., Kwon, J.H., Choi, K.S.,2008. Protectiveefficacy of commercial inactivated Newcastle disease virus vaccines in chickens against a recentKorean epizootic strain. Journal of veterinary science9,295-300.
68. Jiang, Y., Liu, H., Liu, P., Kong, X.,2009. Plasmids driven minigenome rescue system forNewcastle disease virus V4strain. Mol Biol Rep36,1909-1914.
69. Kaleta, E.F., Baldeuf, C.,1988. Newcastle disease in free-living and pet birds. In: Alexander, D.J.(Ed.), Newcastle Disease. Kluwer Academic Publishers, Boston.
70. Kattenbelt, J.A., Meers, J., Gould, A.R.,2006. Genome sequence of the thermostable Newcastledisease virus (strain I-2) reveals a possible phenotypic locus. Vet Microbiol114,134-141.
71. Kawahara, N., Yang, X.Z., Sakaguchi, T., Kiyotani, K., Nagai, Y., Yoshida, T.,1992. Distributionand substrate specificity of intracellular proteolytic processing enzyme(s) for paramyxovirus fusionglycoproteins. J Gen Virol73(Pt3),583-590.
72. Khattar, S.K., Samal, S., Devico, A.L., Collins, P.L., Samal, S.K.,2011. Newcastle disease virusexpressing human immunodeficiency virus type1envelope glycoprotein induces strong mucosaland serum antibody responses in Guinea pigs. J Virol85,10529-10541.
73. Kim, S.-H., Nayak, S., Paldurai, A., Nayak, B., Samuel, A., Aplogan, G.L., Awoume, K.A., Webby,R.J., Ducatez, M.F., Collins, P.L., Samal, S.K.,2012. Complete Genome Sequence of a NovelNewcastle Disease Virus Strain Isolated from a Chicken in West Africa. J Virol86,11394-11395.
74. Kim, S.H., Yan, Y., Samal, S.K.,2009. Role of the cytoplasmic tail amino acid sequences ofNewcastle disease virus hemagglutinin-neuraminidase protein in virion incorporation, cell fusion,and pathogenicity. J Virol83,10250-10255.
75. Krishnamurthy, S., Huang, Z., Samal, S.K.,2000. Recovery of a virulent strain of newcastledisease virus from cloned cDNA: expression of a foreign gene results in growth retardation andattenuation. Virology278,168-182.
76. Krishnamurthy, S., Samal, S.K.,1998. Nucleotide sequences of the trailer, nucleocapsid proteingene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of theentire genome sequence. J Gen Virol79(Pt10),2419-2424.
77. Lamb, R.A., and G. D. Parks.,2007. Paramyxoviridae: the viruses and their replication. In D. M.Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.),Fields virology,5th ed. Lippincott Williams&Wilkins, Philadelphia, PA.
78. Lawson, N.D., Stillman, E.A., Whitt, M.A., Rose, J.K.,1995. Recombinant vesicular stomatitisviruses from DNA. Proc Natl Acad Sci U S A92,4477-4481.
79. Le Mercier, P., Jacob, Y., Tanner, K., Tordo, N.,2002. A novel expression cassette of lyssavirusshows that the distantly related Mokola virus can rescue a defective rabies virus genome. J Virol76,2024-2027.
80. Li, B.Y., Li, X.R., Lan, X., Yin, X.P., Li, Z.Y., Yang, B., Liu, J.X.,2011. Rescue of Newcastledisease virus from cloned cDNA using an RNA polymerase II promoter. Arch Virol156,979-986.
81. Liu, H., Wang, Z., Wang, Y., Sun, C., Zheng, D., Wu, Y.,2008. Characterization of Newcastledisease virus isolated from waterfowl in China. Avian Dis52,150-155.
82. Liu, H., Wang, Z., Wu, Y., Zheng, D., Sun, C., Bi, D., Zuo, Y., Xu, T.,2007. Molecularepidemiological analysis of Newcastle disease virus isolated in China in2005. J Virol Methods140,206-211.
83. Liu, X.F., Wan, H.Q., Ni, X.X., Wu, Y.T., Liu, W.B.,2003. Pathotypical and genotypicalcharacterization of strains of Newcastle disease virus isolated from outbreaks in chicken and gooseflocks in some regions of China during1985-2001. Arch Virol148,1387-1403.
84. Lubiniecki, A.S., Petricciani, J.C.,2001. Recent trends in cell substrate considerations forcontinuous cell lines. Curr Opin Biotechnol12,317-319.
85. Luytjes, W., Krystal, M., Enami, M., Parvin, J.D., Palese, P.,1989. Amplification, expression, andpackaging of foreign gene by influenza virus. Cell59,1107-1113.
86. Markoff, L., Lin, B., Sveda, M., Lai, C.,1984. Glycosylation and surface expression of theinfluenza virus neuraminidase requires the N-terminal hydrophobic region. Molecular and cellularbiology4,8-16.
87. Martin, A., Staeheli, P., Schneider, U.,2006. RNA polymerase II-controlled expression ofantigenomic RNA enhances the rescue efficacies of two different members of the Mononegaviralesindependently of the site of viral genome replication. J Virol80,5708-5715.
88. Mayo, M.A.,2002. Virus taxonomy-Houston2002. Arch Virol147,1071-1076.
89. Mazija, H., Cajavec, S., Ergotic, N., Ciglar-Grozdanic, I., Gottstein, Z., Ragland, W.L.,2010.Immunogenicity and safety of Queensland V4and Ulster2C strains of Newcastle disease virusgiven to maternally immune, newly hatched chickens by nebulization. Avian Dis54,99-103.
90. McGinnes, L.W., Morrison, T.G.,1987. The nucleotide sequence of the gene encoding theNewcastle disease virus membrane protein and comparisons of membrane protein sequences.Virology156,221-228.
91. Mebatsion, T., Koolen, M.J., de Vaan, L.T., de Haas, N., Braber, M., Romer-Oberdorfer, A., vanden Elzen, P., van der Marel, P.,2002. Newcastle disease virus (NDV) marker vaccine: animmunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by aforeign epitope. J Virol76,10138-10146.
92. Mebatsion, T., Verstegen, S., De Vaan, L.T., Romer-Oberdorfer, A., Schrier, C.C.,2001. Arecombinant newcastle disease virus with low-level V protein expression is immunogenic and lackspathogenicity for chicken embryos. J Virol75,420-428.
93. Melanson, V.R., Iorio, R.M.,2006. Addition of N-glycans in the stalk of the Newcastle diseasevirus HN protein blocks its interaction with the F protein and prevents fusion. J Virol80,623-633.
94. Millar, N.S., Chambers, P., Emmerson, P.T.,1986. Nucleotide sequence analysis of thehaemagglutinin-neuraminidase gene of Newcastle disease virus. J Gen Virol67(Pt9),1917-1927.
95. Miller, P.J., Decanini, E.L., Afonso, C.L.,2010. Newcastle disease: evolution of genotypes and therelated diagnostic challenges. Infect Genet Evol10,26-35.
96. Mirza, A.M., Deng, R., Iorio, R.M.,1994. Site-directed mutagenesis of a conserved hexapeptide inthe paramyxovirus hemagglutinin-neuraminidase glycoprotein: effects on antigenic structure andfunction. J Virol68,5093-5099.
97. Muir, W.I., Bryden, W.L., Husband, A.J.,2000. Immunity, vaccination and the avian intestinal tract.Dev Comp Immunol24,325-342.
98. Nagai, Y., Klenk, H.D., Rott, R.,1976. Proteolytic cleavage of the viral glycoproteins and itssignificance for the virulence of Newcastle disease virus. Virology72,494-508.
99. Nakaya, T., Cros, J., Park, M.S., Nakaya, Y., Zheng, H., Sagrera, A., Villar, E., Garcia-Sastre, A.,Palese, P.,2001. Recombinant Newcastle disease virus as a vaccine vector. J Virol75,11868-11873.
100. Nasser, M., Lohr, J.E., Mebratu, G.Y., Zessin, K.H., Baumann, M.P., Ademe, Z.,2000. OralNewcastle disease vaccination trials in Ethiopia. Avian Pathol29,27-34.
101. Neumann, G., Fujii, K., Kino, Y., Kawaoka, Y.,2005. An improved reverse genetics system forinfluenza A virus generation and its implications for vaccine production. Proc Natl Acad Sci U S A102,16825-16829.
102. Olabode, A.O., Ndako, J.A., Echeonwu, G.O., Nwankiti, O.O., Chukwuedo, A.A.,2010. Use ofcracked maize as a carrier for NDV4vaccine in experimental vaccination of chickens. Virol J7,67.
103. Panda, A., Elankumaran, S., Krishnamurthy, S., Huang, Z., Samal, S.K.,2004a. Loss of N-linkedglycosylation from the hemagglutinin-neuraminidase protein alters virulence of Newcastle diseasevirus. J Virol78,4965-4975.
104. Panda, A., Huang, Z., Elankumaran, S., Rockemann, D.D., Samal, S.K.,2004b. Role of fusionprotein cleavage site in the virulence of Newcastle disease virus. Microb Pathog36,1-10.
105. Park, K.H., Huang, T., Correia, F.F., Krystal, M.,1991. Rescue of a foreign gene by Sendai virus.Proc Natl Acad Sci U S A88,5537-5541.
106. Park, M.S., Garcia-Sastre, A., Cros, J.F., Basler, C.F., Palese, P.,2003. Newcastle disease virus Vprotein is a determinant of host range restriction. J Virol77,9522-9532.
107. Peeples, M.E.1991. Paramyxovirus M proteins, In: The Paramyxoviruses. Springer,427-456.
108. Peeters, B.P., de Leeuw, O.S., Koch, G., Gielkens, A.L.,1999. Rescue of Newcastle disease virusfrom cloned cDNA: evidence that cleavability of the fusion protein is a major determinant forvirulence. J Virol73,5001-5009.
109. Peeters, B.P., Gruijthuijsen, Y.K., de Leeuw, O.S., Gielkens, A.L.,2000. Genome replication ofNewcastle disease virus: involvement of the rule-of-six. Arch Virol145,1829-1845.
110. Peeters, B.P., de Leeuw, O.S., Verstegen, I., Koch, G., Gielkens, A.L.,2001. Generation of arecombinant chimeric Newcastle disease virus vaccine that allows serological differentiation betweenvaccinated and infected animals. Vaccine19,1616-1627.
111. Perozo, F., Marcano, R., Afonso, C.L.,2012. Biological and phylogenetic characterization of agenotype VII Newcastle disease virus from Venezuela: efficacy of field vaccination. J ClinMicrobiol50,1204-1208.
112. Perozo, F., Villegas, P., Dolz, R., Afonso, C.L., Purvis, L.B.,2008. The VG/GA strain of Newcastledisease virus: mucosal immunity, protection against lethal challenge and molecular analysis. AvianPathol37,237-245.
113. Picken, J.,1964. Thermostability of Newcastle disease virus. Newcastle Dis-ease Virus: AnEvolving Pathogen, University of Wisconsin Press, Madison and Milwaukee,167-188.
114. Puhler, F., Willuda, J., Puhlmann, J., Mumberg, D., Romer-Oberdorfer, A., Beier, R.,2008.Generation of a recombinant oncolytic Newcastle disease virus and expression of a full IgGantibody from two transgenes. Gene Ther15,371-383.
115. Qin, Z.M., Ma, B.C., He, Y.F., Wang, Y.L., Cui, Z.Z.,2006. Wei Sheng Wu Xue Bao46,227-232.
116. Radecke, F., Spielhofer, P., Schneider, H., Kaelin, K., Huber, M., Dotsch, C., Christiansen, G.,Billeter, M.A.,1995. Rescue of measles viruses from cloned DNA. EMBO J14,5773-5784.
117. Ramp, K., Skiba, M., Karger, A., Mettenleiter, T.C., Romer-Oberdorfer, A.,2011. Influence ofinsertion site of the avian influenza virus haemagglutinin (HA) gene within the Newcastle diseasevirus genome on HA expression. J Gen Virol92,355-360.
118. Rauw, F., Gardin, Y., Palya, V., Van Borm, S., Gonze, M., Lemaire, S., van den Berg, T., Lambrecht,B.,2009. Humoral, cell-mediated and mucosal immunity induced by oculo-nasal vaccination ofone-day-old SPF and conventional layer chicks with two different live Newcastle disease vaccines.Vaccine27,3631-3642.
119. Rehmani, S.F., Spradbrow, P.B., West, R.,1995. Lactose pellets--a new approach to oralvaccination of village chickens against Newcastle disease. Vet Microbiol46,47-53.
120. Ren, G., Wang, Z., Wang, G., Song, Y., Yao, P., Xu, H., Wen, H., Zhang, W.,2006. Effects of heptadrepeat regions of f protein on the specific membrane fusion in paramyxoviruses. Intervirology49,299-306.
121. Rennick, L.J., Duprex, W.P., Rima, B.K.,2007. Measles virus minigenomes encoding twoautofluorescent proteins reveal cell-to-cell variation in reporter expression dependent on viralsequences between the transcription units. J Gen Virol88,2710-2718.
122. Reynolds, D.L., Maraqa, A.D.,2000. Protective immunity against Newcastle disease: the role ofantibodies specific to Newcastle disease virus polypeptides. Avian Dis44,138-144.
123. Romer-Oberdorfer, A., Mundt, E., Mebatsion, T., Buchholz, U.J., Mettenleiter, T.C.,1999.Generation of recombinant lentogenic Newcastle disease virus from cDNA. J Gen Virol80(Pt11),2987-2995.
124. Romer-Oberdorfer, A., Werner, O., Veits, J., Mebatsion, T., Mettenleiter, T.C.,2003. Contributionof the length of the HN protein and the sequence of the F protein cleavage site to Newcastledisease virus pathogenicity. J Gen Virol84,3121-3129.
125. Rout, S.N., Samal, S.K.,2008. The large polymerase protein is associated with the virulence ofNewcastle disease virus. J Virol82,7828-7836.
126. Sakaguchi, T., Matsuda, Y., Kiyokage, R., Kawahara, N., Kiyotani, K., Katunuma, N., Nagai, Y.,Yoshida, T.,1991. Identification of endoprotease activity in the trans Golgi membranes of rat livercells that specifically processes in vitro the fusion glycoprotein precursor of virulent Newcastledisease virus. Virology184,504-512.
127. Samson, A.C.R.,1988. Virus structure. pp:23-45. In: Newcastle disease. D.J.Alexander. Springer.Kluwer academic publishers.
128. Samuel, J.L., Bensink, Z., Spradbrow, P.B.,1993. Oral vaccination of chickens with the V4strain ofNewcastle disease virus. Cooked and raw white rice as a vehicle. Trop Anim Health Prod25,2-10.
129. Schaper, U.M., Fuller, F.J., Ward, M.D., Mehrotra, Y., Stone, H.O., Stripp, B.R., De Buysscher,E.V.,1988. Nucleotide sequence of the envelope protein genes of a highly virulent, neurotropicstrain of Newcastle disease virus. Virology165,291-295.
130. Schirrmacher, V., Fournier, P.,2009. Newcastle disease virus: a promising vector for viral therapy,immune therapy, and gene therapy of cancer. Methods Mol Biol542,565-605.
131. Schloer, G.M., Hanson, R.P.,1968. Relationship of plaque size and virulence for chickens of14representative Newcastle disease virus strains. J Virol2,40-47.
132. Schnell, M.J., Mebatsion, T., Conzelmann, K.K.,1994. Infectious rabies viruses from clonedcDNA. EMBO J13,4195-4203.
133. Schnell, M.J., Buonocore, L., Whitt, M.A., Rose, J.K.,1996. The minimal conserved transcriptionstop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. J Virol70,2318-2323.
134. Simmons, G.C.,1967. The isolation of Newcastle disease virus in Queensland. Aust Vet J43,29-30.
135. Snoeck, C.J., Ducatez, M.F., Owoade, A.A., Faleke, O.O., Alkali, B.R., Tahita, M.C., Tarnagda, Z.,Ouedraogo, J.B., Maikano, I., Mbah, P.O., Kremer, J.R., Muller, C.P.,2009. Newcastle diseasevirus in West Africa: new virulent strains identified in non-commercial farms. Arch Virol154,47-54.
136. Spiess, M., Lodish, H.F.,1986. An internal signal sequence: the asialoglycoprotein receptormembrane anchor. Cell44,177-185.
137. Steward, M., Vipond, I.B., Millar, N.S., Emmerson, P.T.,1993. RNA editing in Newcastle diseasevirus. J Gen Virol74(Pt12),2539-2547.
138. Sun J H., C.D.J.,2001. Indirect ELISA for the Detection of NDV Specific ImmunoglobulinsG, M,and A. Chinese Journal of Preventive Veterinary Medicine23,23-25.
139. Tan, W.S., Lau, C.H., Ng, B.K., Ibrahim, A.L., Yusoff, K.,1995. Nucleotide sequence of thehaemagglutinin-neuraminidase (HN) gene of a Malaysian heat resistant viscerotropic-velogenicNewcastle disease virus (NDV) strain AF2240. DNA Seq6,47-50.
140. Tang, R.S., Nguyen, N., Zhou, H., Jin, H.,2002. Clustered charge-to-alanine mutagenesis ofhuman respiratory syncytial virus L polymerase generates temperature-sensitive viruses. Virology302,207-216.
141. Tsai, H.J., Chang, K.H., Tseng, C.H., Frost, K.M., Manvell, R.J., Alexander, D.J.,2004. Antigenicand genotypical characterization of Newcastle disease viruses isolated in Taiwan between1969and
1996. Vet Microbiol104,19-30.
142. Vigil, A., Park, M.S., Martinez, O., Chua, M.A., Xiao, S., Cros, J.F., Martinez-Sobrido, L., Woo,S.L., Garcia-Sastre, A.,2007. Use of reverse genetics to enhance the oncolytic properties ofNewcastle disease virus. Cancer Res67,8285-8292.
143. Wakamatsu, N., King, D.J., Seal, B.S., Peeters, B.P., Brown, C.C.,2006. The effect onpathogenesis of Newcastle disease virus LaSota strain from a mutation of the fusion cleavage siteto a virulent sequence. Avian Dis50,483-488.
144. Walpita, P., Flick, R.,2005. Reverse genetics of negative-stranded RNA viruses: a globalperspective. FEMS Microbiol Lett244,9-18.
145. Walpita, P., Peters, C.J.,2007. Cis-acting elements in the antigenomic promoter of Nipah virus. JGen Virol88,2542-2551.
146. Wang, Z., Liu, H., Xu, J., Bao, J., Zheng, D., Sun, C., Wei, R., Song, C., Chen, J.,2006.Genotyping of Newcastle disease viruses isolated from2002to2004in China. Ann N Y Acad Sci1081,228-239.
147. Wei, D., Sun, N., Nan, G., Wang, Y., Liu, H.Q., Peeters, B., Chen, Z.N., Bian, H.,2012.Construction of recombinant Newcastle disease virus Italien strain for oncolytic virotherapy oftumors. Hum Gene Ther23,700-710.
148. Weiss, S.R., Bratt, M.A.,1974. Polyadenylate sequences on Newcastle disease virus mRNAsynthesized in vivo and in vitro. J Virol13,1220-1230.
149. Yan, Y., Rout, S.N., Kim, S.H., Samal, S.K.,2009a. Role of untranslated regions of thehemagglutinin-neuraminidase gene in replication and pathogenicity of newcastle disease virus. JVirol83,5943-5946.
150. Yan, Y., Samal, S.K.,2008. Role of intergenic sequences in newcastle disease virus RNAtranscription and pathogenesis. J Virol82,1323-1331.
151. Yan, Y.Q., Rout, S.N., Kim, S.H., Samal, S.K.,2009b. Role of Untranslated Regions of theHemagglutinin-Neuraminidase Gene in Replication and Pathogenicity of Newcastle Disease Virus.J Virol83,5943-5946.
152. Yap, C.C., Ishii, K., Aoki, Y., Aizaki, H., Tani, H., Shimizu, H., Ueno, Y., Miyamura, T., Matsuura,Y.,1997. A hybrid baculovirus-T7RNA polymerase system for recovery of an infectious virusfrom cDNA. Virology231,192-200.
153. Yi, J., Liu, C., Chen, B., Wu, S.,2011. Molecular Characterization of a Virulent Genotype VIIdStrain of Newcastle Disease Virus from Farmed Chickens in Shanghai. Avian Diseases55,279-284.
154. Yusoff, K., Millar, N.S., Chambers, P., Emmerson, P.T.,1987. Nucleotide sequence analysis of theL gene of Newcastle disease virus: homologies with Sendai and vesicular stomatitis viruses.Nucleic Acids Res15,3961-3976.
155. Yusoff, K., Tan, W.S., Lau, C.H., Ng, B.K., Ibrahim, A.L.,1996. Sequence of thehaemagglutinin-neuraminidase gene of the Newcastle disease virus oral vaccine strain V4(UPM).Avian Pathol25,837-844.
156. Zamarin, D., Palese, P.,2012. Oncolytic Newcastle disease virus for cancer therapy: old challengesand new directions. Future Microbiology7,347-367.
157. Zanetti, F., Berinstein, A., Carrillo, E.,2008. Effect of host selective pressure on Newcastle diseasevirus virulence. Microbial Pathogenesis44,135-140.
158. Zhao, H., Peeters, B.P.,2003. Recombinant Newcastle disease virus as a viral vector: effect ofgenomic location of foreign gene on gene expression and virus replication. J Gen Virol84,781-788.
159. Zulkifli, M.M., Ibrahim, R., Ali, A.M., Aini, I., Jaafar, H., Hilda, S.S., Alitheen, N.B., Abdullah,J.M.,2009. Newcastle diseases virus strain V4UPM displayed oncolytic ability againstexperimental human malignant glioma. Neurol Res31,3-10.
2.姜延龙.新城疫病毒V4株全基因组测序及全长cDNA克隆的构建.[姜延龙博士学位论文].黑龙江：东北农业大学,2009.
3.黎秋华,邵华斌，杨峻.鸡新城疫病毒HB92株生物学特性研究(II)—对热的稳定性及在鸡群中的自然传播性试验[J].湖北畜牧兽医,1996,2:1-2.
4.殷震,刘景华(主编).(1997).动物病毒学[M].北京:科学出版社.
5.邵华斌,黎秋华,杨峻.鸡新城疫病毒HB92株生物学特性研究(I)—对鸽、鹌鹑的免疫试验[J].湖北畜牧兽医,1996,1:4-5
6.吴宝成,周金法,孙莉.新城疫V4+C30复合疫苗的制备和免疫研究[J].中国预防兽医学报，2001,23(6):451-454.
7.徐振东,孙莉,周金法.新城疫V4株弱毒变种的选育[J].中国畜禽传染病,1996,6:20-23.
8. Abolnik, C., Horner, R.F., Bisschop, S.P., Parker, M.E., Romito, M., Viljoen, G.J.,2004. Aphylogenetic study of South African Newcastle disease virus strains isolated between1990and2002suggests epidemiological origins in the Far East. Arch Virol149,603-619.
9. Al-Garib, S.O., Gielkens, A.L., Gruys, D.E., Hartog, L., Koch, G.,2003. Immunoglobulin classdistribution of systemic and mucosal antibody responses to Newcastle disease in chickens. AvianDis47,32-40.
10. Alexander, D.,2000. Newcastle disease and other avian paramyxoviruses. Revue Scientifique etTechnique-Office International des Epizooties19,443-455.
11. Altomonte, J., Marozin, S., Schmid, R.M., Ebert, O.,2010. Engineered newcastle disease virus asan improved oncolytic agent against hepatocellular carcinoma. Mol Ther18,275-284.
12. Bailey, D., Chard, L.S., Dash, P., Barrett, T., Banyard, A.C.,2007. Reverse genetics forpeste-des-petits-ruminants virus (PPRV): promoter and protein specificities. Virus Res126,250-255.
13. Baron, M.D., Foster-Cuevas, M., Baron, J., Barrett, T.,1999. Expression in cattle of epitopes of aheterologous virus using a recombinant rinderpest virus. J Gen Virol80(Pt8),2031-2039.
14. Berinstein, A., Vazquez-Rovere, C., Asurmendi, S., Gomez, E., Zanetti, F., Zabal, O., Tozzini, A.,Conte Grand, D., Taboga, O., Calamante, G., Barrios, H., Hopp, E., Carrillo, E.,2005. Mucosal andsystemic immunization elicited by Newcastle disease virus (NDV) transgenic plants as antigens.Vaccine23,5583-5589.
15. Blakqori, G., Kochs, G., Haller, O., Weber, F.,2003. Functional L polymerase of La Crosse virusallows in vivo reconstitution of recombinant nucleocapsids. J Gen Virol84,1207-1214.
16. Blumberg, B.M., Kolakofsky, D.,1981. Intracellular vesicular stomatitis virus leader RNAs arefound in nucleocapsid structures. J Virol40,568-576.
17. Britton, P., Green, P., Kottier, S., Mawditt, K.L., Penzes, Z., Cavanagh, D., Skinner, M.A.,1996.Expression of bacteriophage T7RNA polymerase in avian and mammalian cells by a recombinantfowlpox virus. J Gen Virol77(Pt5),963-967.
18. Brown, D.D., Collins, F.M., Duprex, W.P., Baron, M.D., Barrett, T., Rima, B.K.,2005.'Rescue' ofmini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins. J GenVirol86,1077-1081.
19. Brown, P.A., Lupini, C., Catelli, E., Clubbe, J., Ricchizzi, E., Naylor, C.J.,2011. A singlepolymerase (L) mutation in avian metapneumovirus increased virulence and partially maintainedvirus viability at an elevated temperature. J Gen Virol92,346-354.
20. Buchholz, U.J., Finke, S., Conzelmann, K.K.,1999. Generation of bovine respiratory syncytialvirus (BRSV) from cDNA: BRSV NS2is not essential for virus replication in tissue culture, andthe human RSV leader region acts as a functional BRSV genome promoter. J Virol73,251-259.
21. Buckland, R., and F.Wild.,1989. Leucine zipper motif extends. Nature,338:547.
22. Bukreyev, A., Camargo, E., Collins, P.L.,1996. Recovery of infectious respiratory syncytial virusexpressing an additional, foreign gene. J Virol70,6634-6641.
23. Bukreyev, A., Huang, Z., Yang, L., Elankumaran, S., St Claire, M., Murphy, B.R., Samal, S.K.,Collins, P.L.,2005. Recombinant newcastle disease virus expressing a foreign viral antigen isattenuated and highly immunogenic in primates. J Virol79,13275-13284.
24. Carr, C.M., Chaudhry, C., Kim, P.S.,1997. Influenza hemagglutinin is spring-loaded by ametastable native conformation. Proc Natl Acad Sci U S A94,14306-14313.
25. Cattaneo, R., Rebmann, G., Schmid, A., Baczko, K., ter Meulen, V., Billeter, M.A.,1987. Alteredtranscription of a defective measles virus genome derived from a diseased human brain. EMBO J6,681-688.
26. Chambers, P., Millar, N.S., Bingham, R.W., Emmerson, P.T.,1986. Molecular cloning ofcomplementary DNA to Newcastle disease virus, and nucleotide sequence analysis of the junctionbetween the genes encoding the haemagglutinin-neuraminidase and the large protein. J Gen Virol67(Pt3),475-486.
27. Chambers, P., Pringle, C.R., Easton, A.J.,1990. Heptad repeat sequences are located adjacent tohydrophobic regions in several types of virus fusion glycoproteins. J Gen Virol71(Pt12),3075-3080.
28. Chen, F., Liu, J., Liu, D., Yan, Z., Ji, J., Qin, J., Li, H., Ma, J., Bi, Y., Xie, Q.,2012. CompleteGenome Sequence of a Newcastle Disease Virus Strain Isolated from Broiler Breeder Flocks inChina. J Virol86,12461-12462.
29. Chen, L., Gorman, J.J., McKimm-Breschkin, J., Lawrence, L.J., Tulloch, P.A., Smith, B.J., Colman,P.M., Lawrence, M.C.,2001. The structure of the fusion glycoprotein of Newcastle disease virussuggests a novel paradigm for the molecular mechanism of membrane fusion. Structure9,255-266.
30. Collins, M.S., Bashiruddin, J.B., Alexander, D.J.,1993. Deduced amino acid sequences at thefusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity andpathogenicity. Arch Virol128,363-370.
31. Courtney, S.C., Gomez, D., Susta, L., Hines, N., Pedersen, J.C., Miller, P.J., Afonso, C.L.,2012.Complete genome sequencing of a novel newcastle disease virus isolate circulating in layerchickens in the dominican republic. J Virol86,9550.
32. Crennell, S., Takimoto, T., Portner, A., Taylor, G.,2000. Crystal structure of the multifunctionalparamyxovirus hemagglutinin-neuraminidase. Nat Struct Biol7,1068-1074.
33. Curran, J., Boeck, R., Kolakofsky, D.,1991. The Sendai virus P gene expresses both an essentialprotein and an inhibitor of RNA synthesis by shuffling modules via mRNA editing. EMBO J10,3079-3085.
34. Curran, J., Marq, J.B., Kolakofsky, D.,1995. An N-terminal domain of the Sendai paramyxovirus Pprotein acts as a chaperone for the NP protein during the nascent chain assembly step of genomereplication. J Virol69,849-855.
35. Czegledi, A., Ujvari, D., Somogyi, E., Wehmann, E., Werner, O., Lomniczi, B.,2006. Thirdgenome size category of avian paramyxovirus serotype1(Newcastle disease virus) andevolutionary implications. Virus Res120,36-48.
36. de Leeuw, O.S., Koch, G., Hartog, L., Ravenshorst, N., Peeters, B.P.,2005. Virulence of Newcastledisease virus is determined by the cleavage site of the fusion protein and by both the stem regionand globular head of the haemagglutinin-neuraminidase protein. J Gen Virol86,1759-1769.
37. DiNapoli, J.M., Kotelkin, A., Yang, L., Elankumaran, S., Murphy, B.R., Samal, S.K., Collins, P.L.,Bukreyev, A.,2007. Newcastle disease virus, a host range-restricted virus, as a vaccine vector forintranasal immunization against emerging pathogens. Proc Natl Acad Sci U S A104,9788-9793.
38. DiNapoli, J.M., Nayak, B., Yang, L., Finneyfrock, B.W., Cook, A., Andersen, H., Torres-Velez, F.,Murphy, B.R., Samal, S.K., Collins, P.L., Bukreyev, A.,2010.
39. Foster, H.A., Chitukuro, H.R., Tuppa, E., Mwanjala, T., Kusila, C.,1999. Thermostable Newcastledisease vaccines in Tanzania. Vet Microbiol68,127-130.
40. Newcastle disease virus-vectored vaccines expressing the hemagglutinin or neuraminidase proteinof H5N1highly pathogenic avian influenza virus protect against virus challenge in monkeys. JVirol84,1489-1503.
41. DJ Cao, J., Sun, LH,Yan, PX, Liu et al. July15-20,2001. the changes of IgG, IgM and IgAantibody level of chickens immunized with NDV LaSota or V4vaccine strain. In: Proceedings ofthe Sixth International Veterinary Immunology Symposium, Uppsala, Sweden, p102.
42. Dortmans, J.C., Koch, G., Rottier, P.J., Peeters, B.P.,2009. Virulence of pigeon paramyxovirus type1does not always correlate with the cleavability of its fusion protein. J Gen Virol90,2746-2750.
43. Dortmans, J.C., Rottier, P.J., Koch, G., Peeters, B.P.,2010. The viral replication complex isassociated with the virulence of Newcastle disease virus. J Virol84,10113-10120.
44. Dortmans, J.C., Rottier, P.J., Koch, G., Peeters, B.P.,2011. Passaging of a Newcastle disease viruspigeon variant in chickens results in selection of viruses with mutations in the polymerase complexenhancing virus replication and virulence. J Gen Virol92,336-345.
45. Egelman, E.H., Wu, S.S., Amrein, M., Portner, A., Murti, G.,1989. The Sendai virus nucleocapsidexists in at least four different helical states. J Virol63,2233-2243.
46. Errington, W., Emmerson, P.T.,1997. Assembly of recombinant Newcastle disease virusnucleocapsid protein into nucleocapsid-like structures is inhibited by the phosphoprotein. J GenVirol78,2335-2339.
47. Errington, W., Steward, M., Emmerson, P.T.,1995. A diagnostic immunoassay for Newcastledisease virus based on the nucleocapsid protein expressed by a recombinant baculovirus. Journal ofvirological methods55,357-365.
48. Estevez, C., King, D., Seal, B., Yu, Q.,2007. Evaluation of Newcastle disease virus chimerasexpressing the Hemagglutinin-Neuraminidase protein of velogenic strains in the context of amesogenic recombinant virus backbone. Virus Res129,182-190.
49. Freiberg, A., Dolores, L.K., Enterlein, S., Flick, R.,2008. Establishment and characterization ofplasmid-driven minigenome rescue systems for Nipah virus: RNA polymerase I-and T7-catalyzedgeneration of functional paramyxoviral RNA. Virology370,33-44.
50. Garcin, D., Pelet, T., Calain, P., Roux, L., Curran, J., Kolakofsky, D.,1995. A highlyrecombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA:generation of a novel copy-back nondefective interfering virus. EMBO J14,6087-6094.
51. Ge, J., Deng, G., Wen, Z., Tian, G., Wang, Y., Shi, J., Wang, X., Li, Y., Hu, S., Jiang, Y., Yang, C.,Yu, K., Bu, Z., Chen, H.,2007. Newcastle disease virus-based live attenuated vaccine completelyprotects chickens and mice from lethal challenge of homologous and heterologous H5N1avianinfluenza viruses. J Virol81,150-158.
52. Groseth, A., Feldmann, H., Theriault, S., Mehmetoglu, G., Flick, R.,2005. RNA polymeraseI-driven minigenome system for Ebola viruses. J Virol79,4425-4433.
53. Haller, A.A., MacPhail, M., Mitiku, M., Tang, R.S.,2001. A single amino acid substitution in theviral polymerase creates a temperature-sensitive and attenuated recombinant bovine parainfluenzavirus type3. Virology288,342-350.
54. Hamaguchi, M., Yoshida, T., Nishikawa, K., Naruse, H., Nagai, Y.,1983. Transcriptive complex ofNewcastle disease virus. I. Both L and P proteins are required to constitute an active complex.Virology128,105-117.
55. He, B., Paterson, R.G., Ward, C.D., Lamb, R.A.,1997. Recovery of infectious SV5from clonedDNA and expression of a foreign gene. Virology237,249-260.
56. Hu, H., Roth, J.P., Estevez, C.N., Zsak, L., Liu, B., Yu, Q.,2011. Generation and evaluation of arecombinant Newcastle disease virus expressing the glycoprotein (G) of avian metapneumovirussubgroup C as a bivalent vaccine in turkeys. Vaccine29,8624-8633.
57. Hu, S., Ma, H., Wu, Y., Liu, W., Wang, X., Liu, Y., Liu, X.,2009. A vaccine candidate of attenuatedgenotype VII Newcastle disease virus generated by reverse genetics. Vaccine27,904-910.
58. Huang, Y., Wan, H.Q., Liu, H.Q., Wu, Y.T., Liu, X.F.,2004a. Genomic sequence of an isolate ofNewcastle disease virus isolated from an outbreak in geese: a novel six nucleotide insertion in thenon-coding region of the nucleoprotein gene. Brief Report. Arch Virol149,1445-1457.
59. Huang, Z., Elankumaran, S., Yunus, A.S., Samal, S.K.,2004b. A recombinant Newcastle diseasevirus (NDV) expressing VP2protein of infectious bursal disease virus (IBDV) protects againstNDV and IBDV. J Virol78,10054-10063.
60. Huang, Z., Krishnamurthy, S., Panda, A., Samal, S.K.,2003. Newcastle disease virus V protein isassociated with viral pathogenesis and functions as an alpha interferon antagonist. J Virol77,8676-8685.
61. Huang, Z., Panda, A., Elankumaran, S., Govindarajan, D., Rockemann, D.D., Samal, S.K.,2004c.The hemagglutinin-neuraminidase protein of Newcastle disease virus determines tropism andvirulence. J Virol78,4176-4184.
62. Ibrahim, A.L., Chulan, U., Babjee, A.M.,1981. An assessment of the Australian V4strain ofNewcastle disease virus as a vaccine by spray, aerosol and drinking water administration. Aust VetJ57,277-280.
63. Ideris, A., Ibrahim, A.L., Spradbrow, P.B.,1990. Vaccination of chickens against Newcastle diseasewith a food pellet vaccine. Avian Pathol19,371-384.
64. Inoue, K., Shoji, Y., Kurane, I., Iijima, T., Sakai, T., Morimoto, K.,2003. An improved method forrecovering rabies virus from cloned cDNA. J Virol Methods107,229-236.
65. Jayawardane, G.W., Spradbrow, P.B.,1995a. Cell-mediated immunity in chickens vaccinated withthe V4strain of Newcastle disease virus. Vet Microbiol46,37-41.
66. Jayawardane, G.W., Spradbrow, P.B.,1995b. Mucosal immunity in chickens vaccinated with theV4strain of Newcastle disease virus. Vet Microbiol46,69-77.
67. Jeon, W.J., Lee, E.K., Lee, Y.J., Jeong, O.M., Kim, Y.J., Kwon, J.H., Choi, K.S.,2008. Protectiveefficacy of commercial inactivated Newcastle disease virus vaccines in chickens against a recentKorean epizootic strain. Journal of veterinary science9,295-300.
68. Jiang, Y., Liu, H., Liu, P., Kong, X.,2009. Plasmids driven minigenome rescue system forNewcastle disease virus V4strain. Mol Biol Rep36,1909-1914.
69. Kaleta, E.F., Baldeuf, C.,1988. Newcastle disease in free-living and pet birds. In: Alexander, D.J.(Ed.), Newcastle Disease. Kluwer Academic Publishers, Boston.
70. Kattenbelt, J.A., Meers, J., Gould, A.R.,2006. Genome sequence of the thermostable Newcastledisease virus (strain I-2) reveals a possible phenotypic locus. Vet Microbiol114,134-141.
71. Kawahara, N., Yang, X.Z., Sakaguchi, T., Kiyotani, K., Nagai, Y., Yoshida, T.,1992. Distributionand substrate specificity of intracellular proteolytic processing enzyme(s) for paramyxovirus fusionglycoproteins. J Gen Virol73(Pt3),583-590.
72. Khattar, S.K., Samal, S., Devico, A.L., Collins, P.L., Samal, S.K.,2011. Newcastle disease virusexpressing human immunodeficiency virus type1envelope glycoprotein induces strong mucosaland serum antibody responses in Guinea pigs. J Virol85,10529-10541.
73. Kim, S.-H., Nayak, S., Paldurai, A., Nayak, B., Samuel, A., Aplogan, G.L., Awoume, K.A., Webby,R.J., Ducatez, M.F., Collins, P.L., Samal, S.K.,2012. Complete Genome Sequence of a NovelNewcastle Disease Virus Strain Isolated from a Chicken in West Africa. J Virol86,11394-11395.
74. Kim, S.H., Yan, Y., Samal, S.K.,2009. Role of the cytoplasmic tail amino acid sequences ofNewcastle disease virus hemagglutinin-neuraminidase protein in virion incorporation, cell fusion,and pathogenicity. J Virol83,10250-10255.
75. Krishnamurthy, S., Huang, Z., Samal, S.K.,2000. Recovery of a virulent strain of newcastledisease virus from cloned cDNA: expression of a foreign gene results in growth retardation andattenuation. Virology278,168-182.
76. Krishnamurthy, S., Samal, S.K.,1998. Nucleotide sequences of the trailer, nucleocapsid proteingene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of theentire genome sequence. J Gen Virol79(Pt10),2419-2424.
77. Lamb, R.A., and G. D. Parks.,2007. Paramyxoviridae: the viruses and their replication. In D. M.Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.),Fields virology,5th ed. Lippincott Williams&Wilkins, Philadelphia, PA.
78. Lawson, N.D., Stillman, E.A., Whitt, M.A., Rose, J.K.,1995. Recombinant vesicular stomatitisviruses from DNA. Proc Natl Acad Sci U S A92,4477-4481.
79. Le Mercier, P., Jacob, Y., Tanner, K., Tordo, N.,2002. A novel expression cassette of lyssavirusshows that the distantly related Mokola virus can rescue a defective rabies virus genome. J Virol76,2024-2027.
80. Li, B.Y., Li, X.R., Lan, X., Yin, X.P., Li, Z.Y., Yang, B., Liu, J.X.,2011. Rescue of Newcastledisease virus from cloned cDNA using an RNA polymerase II promoter. Arch Virol156,979-986.
81. Liu, H., Wang, Z., Wang, Y., Sun, C., Zheng, D., Wu, Y.,2008. Characterization of Newcastledisease virus isolated from waterfowl in China. Avian Dis52,150-155.
82. Liu, H., Wang, Z., Wu, Y., Zheng, D., Sun, C., Bi, D., Zuo, Y., Xu, T.,2007. Molecularepidemiological analysis of Newcastle disease virus isolated in China in2005. J Virol Methods140,206-211.
83. Liu, X.F., Wan, H.Q., Ni, X.X., Wu, Y.T., Liu, W.B.,2003. Pathotypical and genotypicalcharacterization of strains of Newcastle disease virus isolated from outbreaks in chicken and gooseflocks in some regions of China during1985-2001. Arch Virol148,1387-1403.
84. Lubiniecki, A.S., Petricciani, J.C.,2001. Recent trends in cell substrate considerations forcontinuous cell lines. Curr Opin Biotechnol12,317-319.
85. Luytjes, W., Krystal, M., Enami, M., Parvin, J.D., Palese, P.,1989. Amplification, expression, andpackaging of foreign gene by influenza virus. Cell59,1107-1113.
86. Markoff, L., Lin, B., Sveda, M., Lai, C.,1984. Glycosylation and surface expression of theinfluenza virus neuraminidase requires the N-terminal hydrophobic region. Molecular and cellularbiology4,8-16.
87. Martin, A., Staeheli, P., Schneider, U.,2006. RNA polymerase II-controlled expression ofantigenomic RNA enhances the rescue efficacies of two different members of the Mononegaviralesindependently of the site of viral genome replication. J Virol80,5708-5715.
88. Mayo, M.A.,2002. Virus taxonomy-Houston2002. Arch Virol147,1071-1076.
89. Mazija, H., Cajavec, S., Ergotic, N., Ciglar-Grozdanic, I., Gottstein, Z., Ragland, W.L.,2010.Immunogenicity and safety of Queensland V4and Ulster2C strains of Newcastle disease virusgiven to maternally immune, newly hatched chickens by nebulization. Avian Dis54,99-103.
90. McGinnes, L.W., Morrison, T.G.,1987. The nucleotide sequence of the gene encoding theNewcastle disease virus membrane protein and comparisons of membrane protein sequences.Virology156,221-228.
91. Mebatsion, T., Koolen, M.J., de Vaan, L.T., de Haas, N., Braber, M., Romer-Oberdorfer, A., vanden Elzen, P., van der Marel, P.,2002. Newcastle disease virus (NDV) marker vaccine: animmunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by aforeign epitope. J Virol76,10138-10146.
92. Mebatsion, T., Verstegen, S., De Vaan, L.T., Romer-Oberdorfer, A., Schrier, C.C.,2001. Arecombinant newcastle disease virus with low-level V protein expression is immunogenic and lackspathogenicity for chicken embryos. J Virol75,420-428.
93. Melanson, V.R., Iorio, R.M.,2006. Addition of N-glycans in the stalk of the Newcastle diseasevirus HN protein blocks its interaction with the F protein and prevents fusion. J Virol80,623-633.
94. Millar, N.S., Chambers, P., Emmerson, P.T.,1986. Nucleotide sequence analysis of thehaemagglutinin-neuraminidase gene of Newcastle disease virus. J Gen Virol67(Pt9),1917-1927.
95. Miller, P.J., Decanini, E.L., Afonso, C.L.,2010. Newcastle disease: evolution of genotypes and therelated diagnostic challenges. Infect Genet Evol10,26-35.
96. Mirza, A.M., Deng, R., Iorio, R.M.,1994. Site-directed mutagenesis of a conserved hexapeptide inthe paramyxovirus hemagglutinin-neuraminidase glycoprotein: effects on antigenic structure andfunction. J Virol68,5093-5099.
97. Muir, W.I., Bryden, W.L., Husband, A.J.,2000. Immunity, vaccination and the avian intestinal tract.Dev Comp Immunol24,325-342.
98. Nagai, Y., Klenk, H.D., Rott, R.,1976. Proteolytic cleavage of the viral glycoproteins and itssignificance for the virulence of Newcastle disease virus. Virology72,494-508.
99. Nakaya, T., Cros, J., Park, M.S., Nakaya, Y., Zheng, H., Sagrera, A., Villar, E., Garcia-Sastre, A.,Palese, P.,2001. Recombinant Newcastle disease virus as a vaccine vector. J Virol75,11868-11873.
100. Nasser, M., Lohr, J.E., Mebratu, G.Y., Zessin, K.H., Baumann, M.P., Ademe, Z.,2000. OralNewcastle disease vaccination trials in Ethiopia. Avian Pathol29,27-34.
101. Neumann, G., Fujii, K., Kino, Y., Kawaoka, Y.,2005. An improved reverse genetics system forinfluenza A virus generation and its implications for vaccine production. Proc Natl Acad Sci U S A102,16825-16829.
102. Olabode, A.O., Ndako, J.A., Echeonwu, G.O., Nwankiti, O.O., Chukwuedo, A.A.,2010. Use ofcracked maize as a carrier for NDV4vaccine in experimental vaccination of chickens. Virol J7,67.
103. Panda, A., Elankumaran, S., Krishnamurthy, S., Huang, Z., Samal, S.K.,2004a. Loss of N-linkedglycosylation from the hemagglutinin-neuraminidase protein alters virulence of Newcastle diseasevirus. J Virol78,4965-4975.
104. Panda, A., Huang, Z., Elankumaran, S., Rockemann, D.D., Samal, S.K.,2004b. Role of fusionprotein cleavage site in the virulence of Newcastle disease virus. Microb Pathog36,1-10.
105. Park, K.H., Huang, T., Correia, F.F., Krystal, M.,1991. Rescue of a foreign gene by Sendai virus.Proc Natl Acad Sci U S A88,5537-5541.
106. Park, M.S., Garcia-Sastre, A., Cros, J.F., Basler, C.F., Palese, P.,2003. Newcastle disease virus Vprotein is a determinant of host range restriction. J Virol77,9522-9532.
107. Peeples, M.E.1991. Paramyxovirus M proteins, In: The Paramyxoviruses. Springer,427-456.
108. Peeters, B.P., de Leeuw, O.S., Koch, G., Gielkens, A.L.,1999. Rescue of Newcastle disease virusfrom cloned cDNA: evidence that cleavability of the fusion protein is a major determinant forvirulence. J Virol73,5001-5009.
109. Peeters, B.P., Gruijthuijsen, Y.K., de Leeuw, O.S., Gielkens, A.L.,2000. Genome replication ofNewcastle disease virus: involvement of the rule-of-six. Arch Virol145,1829-1845.
110. Peeters, B.P., de Leeuw, O.S., Verstegen, I., Koch, G., Gielkens, A.L.,2001. Generation of arecombinant chimeric Newcastle disease virus vaccine that allows serological differentiation betweenvaccinated and infected animals. Vaccine19,1616-1627.
111. Perozo, F., Marcano, R., Afonso, C.L.,2012. Biological and phylogenetic characterization of agenotype VII Newcastle disease virus from Venezuela: efficacy of field vaccination. J ClinMicrobiol50,1204-1208.
112. Perozo, F., Villegas, P., Dolz, R., Afonso, C.L., Purvis, L.B.,2008. The VG/GA strain of Newcastledisease virus: mucosal immunity, protection against lethal challenge and molecular analysis. AvianPathol37,237-245.
113. Picken, J.,1964. Thermostability of Newcastle disease virus. Newcastle Dis-ease Virus: AnEvolving Pathogen, University of Wisconsin Press, Madison and Milwaukee,167-188.
114. Puhler, F., Willuda, J., Puhlmann, J., Mumberg, D., Romer-Oberdorfer, A., Beier, R.,2008.Generation of a recombinant oncolytic Newcastle disease virus and expression of a full IgGantibody from two transgenes. Gene Ther15,371-383.
115. Qin, Z.M., Ma, B.C., He, Y.F., Wang, Y.L., Cui, Z.Z.,2006. Wei Sheng Wu Xue Bao46,227-232.
116. Radecke, F., Spielhofer, P., Schneider, H., Kaelin, K., Huber, M., Dotsch, C., Christiansen, G.,Billeter, M.A.,1995. Rescue of measles viruses from cloned DNA. EMBO J14,5773-5784.
117. Ramp, K., Skiba, M., Karger, A., Mettenleiter, T.C., Romer-Oberdorfer, A.,2011. Influence ofinsertion site of the avian influenza virus haemagglutinin (HA) gene within the Newcastle diseasevirus genome on HA expression. J Gen Virol92,355-360.
118. Rauw, F., Gardin, Y., Palya, V., Van Borm, S., Gonze, M., Lemaire, S., van den Berg, T., Lambrecht,B.,2009. Humoral, cell-mediated and mucosal immunity induced by oculo-nasal vaccination ofone-day-old SPF and conventional layer chicks with two different live Newcastle disease vaccines.Vaccine27,3631-3642.
119. Rehmani, S.F., Spradbrow, P.B., West, R.,1995. Lactose pellets--a new approach to oralvaccination of village chickens against Newcastle disease. Vet Microbiol46,47-53.
120. Ren, G., Wang, Z., Wang, G., Song, Y., Yao, P., Xu, H., Wen, H., Zhang, W.,2006. Effects of heptadrepeat regions of f protein on the specific membrane fusion in paramyxoviruses. Intervirology49,299-306.
121. Rennick, L.J., Duprex, W.P., Rima, B.K.,2007. Measles virus minigenomes encoding twoautofluorescent proteins reveal cell-to-cell variation in reporter expression dependent on viralsequences between the transcription units. J Gen Virol88,2710-2718.
122. Reynolds, D.L., Maraqa, A.D.,2000. Protective immunity against Newcastle disease: the role ofantibodies specific to Newcastle disease virus polypeptides. Avian Dis44,138-144.
123. Romer-Oberdorfer, A., Mundt, E., Mebatsion, T., Buchholz, U.J., Mettenleiter, T.C.,1999.Generation of recombinant lentogenic Newcastle disease virus from cDNA. J Gen Virol80(Pt11),2987-2995.
124. Romer-Oberdorfer, A., Werner, O., Veits, J., Mebatsion, T., Mettenleiter, T.C.,2003. Contributionof the length of the HN protein and the sequence of the F protein cleavage site to Newcastledisease virus pathogenicity. J Gen Virol84,3121-3129.
125. Rout, S.N., Samal, S.K.,2008. The large polymerase protein is associated with the virulence ofNewcastle disease virus. J Virol82,7828-7836.
126. Sakaguchi, T., Matsuda, Y., Kiyokage, R., Kawahara, N., Kiyotani, K., Katunuma, N., Nagai, Y.,Yoshida, T.,1991. Identification of endoprotease activity in the trans Golgi membranes of rat livercells that specifically processes in vitro the fusion glycoprotein precursor of virulent Newcastledisease virus. Virology184,504-512.
127. Samson, A.C.R.,1988. Virus structure. pp:23-45. In: Newcastle disease. D.J.Alexander. Springer.Kluwer academic publishers.
128. Samuel, J.L., Bensink, Z., Spradbrow, P.B.,1993. Oral vaccination of chickens with the V4strain ofNewcastle disease virus. Cooked and raw white rice as a vehicle. Trop Anim Health Prod25,2-10.
129. Schaper, U.M., Fuller, F.J., Ward, M.D., Mehrotra, Y., Stone, H.O., Stripp, B.R., De Buysscher,E.V.,1988. Nucleotide sequence of the envelope protein genes of a highly virulent, neurotropicstrain of Newcastle disease virus. Virology165,291-295.
130. Schirrmacher, V., Fournier, P.,2009. Newcastle disease virus: a promising vector for viral therapy,immune therapy, and gene therapy of cancer. Methods Mol Biol542,565-605.
131. Schloer, G.M., Hanson, R.P.,1968. Relationship of plaque size and virulence for chickens of14representative Newcastle disease virus strains. J Virol2,40-47.
132. Schnell, M.J., Mebatsion, T., Conzelmann, K.K.,1994. Infectious rabies viruses from clonedcDNA. EMBO J13,4195-4203.
133. Schnell, M.J., Buonocore, L., Whitt, M.A., Rose, J.K.,1996. The minimal conserved transcriptionstop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. J Virol70,2318-2323.
134. Simmons, G.C.,1967. The isolation of Newcastle disease virus in Queensland. Aust Vet J43,29-30.
135. Snoeck, C.J., Ducatez, M.F., Owoade, A.A., Faleke, O.O., Alkali, B.R., Tahita, M.C., Tarnagda, Z.,Ouedraogo, J.B., Maikano, I., Mbah, P.O., Kremer, J.R., Muller, C.P.,2009. Newcastle diseasevirus in West Africa: new virulent strains identified in non-commercial farms. Arch Virol154,47-54.
136. Spiess, M., Lodish, H.F.,1986. An internal signal sequence: the asialoglycoprotein receptormembrane anchor. Cell44,177-185.
137. Steward, M., Vipond, I.B., Millar, N.S., Emmerson, P.T.,1993. RNA editing in Newcastle diseasevirus. J Gen Virol74(Pt12),2539-2547.
138. Sun J H., C.D.J.,2001. Indirect ELISA for the Detection of NDV Specific ImmunoglobulinsG, M,and A. Chinese Journal of Preventive Veterinary Medicine23,23-25.
139. Tan, W.S., Lau, C.H., Ng, B.K., Ibrahim, A.L., Yusoff, K.,1995. Nucleotide sequence of thehaemagglutinin-neuraminidase (HN) gene of a Malaysian heat resistant viscerotropic-velogenicNewcastle disease virus (NDV) strain AF2240. DNA Seq6,47-50.
140. Tang, R.S., Nguyen, N., Zhou, H., Jin, H.,2002. Clustered charge-to-alanine mutagenesis ofhuman respiratory syncytial virus L polymerase generates temperature-sensitive viruses. Virology302,207-216.
141. Tsai, H.J., Chang, K.H., Tseng, C.H., Frost, K.M., Manvell, R.J., Alexander, D.J.,2004. Antigenicand genotypical characterization of Newcastle disease viruses isolated in Taiwan between1969and
1996. Vet Microbiol104,19-30.
142. Vigil, A., Park, M.S., Martinez, O., Chua, M.A., Xiao, S., Cros, J.F., Martinez-Sobrido, L., Woo,S.L., Garcia-Sastre, A.,2007. Use of reverse genetics to enhance the oncolytic properties ofNewcastle disease virus. Cancer Res67,8285-8292.
143. Wakamatsu, N., King, D.J., Seal, B.S., Peeters, B.P., Brown, C.C.,2006. The effect onpathogenesis of Newcastle disease virus LaSota strain from a mutation of the fusion cleavage siteto a virulent sequence. Avian Dis50,483-488.
144. Walpita, P., Flick, R.,2005. Reverse genetics of negative-stranded RNA viruses: a globalperspective. FEMS Microbiol Lett244,9-18.
145. Walpita, P., Peters, C.J.,2007. Cis-acting elements in the antigenomic promoter of Nipah virus. JGen Virol88,2542-2551.
146. Wang, Z., Liu, H., Xu, J., Bao, J., Zheng, D., Sun, C., Wei, R., Song, C., Chen, J.,2006.Genotyping of Newcastle disease viruses isolated from2002to2004in China. Ann N Y Acad Sci1081,228-239.
147. Wei, D., Sun, N., Nan, G., Wang, Y., Liu, H.Q., Peeters, B., Chen, Z.N., Bian, H.,2012.Construction of recombinant Newcastle disease virus Italien strain for oncolytic virotherapy oftumors. Hum Gene Ther23,700-710.
148. Weiss, S.R., Bratt, M.A.,1974. Polyadenylate sequences on Newcastle disease virus mRNAsynthesized in vivo and in vitro. J Virol13,1220-1230.
149. Yan, Y., Rout, S.N., Kim, S.H., Samal, S.K.,2009a. Role of untranslated regions of thehemagglutinin-neuraminidase gene in replication and pathogenicity of newcastle disease virus. JVirol83,5943-5946.
150. Yan, Y., Samal, S.K.,2008. Role of intergenic sequences in newcastle disease virus RNAtranscription and pathogenesis. J Virol82,1323-1331.
151. Yan, Y.Q., Rout, S.N., Kim, S.H., Samal, S.K.,2009b. Role of Untranslated Regions of theHemagglutinin-Neuraminidase Gene in Replication and Pathogenicity of Newcastle Disease Virus.J Virol83,5943-5946.
152. Yap, C.C., Ishii, K., Aoki, Y., Aizaki, H., Tani, H., Shimizu, H., Ueno, Y., Miyamura, T., Matsuura,Y.,1997. A hybrid baculovirus-T7RNA polymerase system for recovery of an infectious virusfrom cDNA. Virology231,192-200.
153. Yi, J., Liu, C., Chen, B., Wu, S.,2011. Molecular Characterization of a Virulent Genotype VIIdStrain of Newcastle Disease Virus from Farmed Chickens in Shanghai. Avian Diseases55,279-284.
154. Yusoff, K., Millar, N.S., Chambers, P., Emmerson, P.T.,1987. Nucleotide sequence analysis of theL gene of Newcastle disease virus: homologies with Sendai and vesicular stomatitis viruses.Nucleic Acids Res15,3961-3976.
155. Yusoff, K., Tan, W.S., Lau, C.H., Ng, B.K., Ibrahim, A.L.,1996. Sequence of thehaemagglutinin-neuraminidase gene of the Newcastle disease virus oral vaccine strain V4(UPM).Avian Pathol25,837-844.
156. Zamarin, D., Palese, P.,2012. Oncolytic Newcastle disease virus for cancer therapy: old challengesand new directions. Future Microbiology7,347-367.
157. Zanetti, F., Berinstein, A., Carrillo, E.,2008. Effect of host selective pressure on Newcastle diseasevirus virulence. Microbial Pathogenesis44,135-140.
158. Zhao, H., Peeters, B.P.,2003. Recombinant Newcastle disease virus as a viral vector: effect ofgenomic location of foreign gene on gene expression and virus replication. J Gen Virol84,781-788.
159. Zulkifli, M.M., Ibrahim, R., Ali, A.M., Aini, I., Jaafar, H., Hilda, S.S., Alitheen, N.B., Abdullah,J.M.,2009. Newcastle diseases virus strain V4UPM displayed oncolytic ability againstexperimental human malignant glioma. Neurol Res31,3-10.
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