黄芩对单一雌激素作用青春期大鼠子宫内膜TF、PAI-1的影响
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摘要
目的：探讨黄芩对单一雌激素全周期持续作用下的青春期大鼠子宫内膜组织因子(tissue factor, TF)及纤溶酶原激活物抑制物-1(Plasminogen activator inhibitor-1, PAI-1)表达的影响,旨在探讨黄芩对单一雌激素全周期持续作用下的无排卵型功能性子宫出血的子宫内膜持续性增生与内膜稳定性与出血相关指标研究,而TF、PAI-1是其相关指标之一。
方法：将56只大鼠随机分为2组,空白组20只灌胃生理盐水,模型组36只灌胃补佳乐,连续灌胃3周,即全周期雌激素干预。其中每组每周处死4只大鼠,取双侧子宫(用4%的多聚甲醛液固定),低温保存。第4周开始将模型组分为生理盐水组8只,雌激素组8只,黄芩组8只。生理盐水组灌胃生理盐水,雌激素组灌胃补佳乐,黄芩组灌胃黄芩悬液。每周各组处死4只大鼠,取双侧子宫(用4%的多聚甲醛液固定),低温保存。各组灌胃剂量按照正常人体体表面积比值计算。通过光镜观察每周各组大鼠子宫内膜形态学的变化。通过免疫组化法检测每周各组大鼠子宫内膜组织因子及纤溶酶原激活物抑制物在子宫内膜的表达情况。
结果：实验结果显示：与空白组相比,模型组前三周每周子宫内膜腺体和间质增生,子宫内膜组织因子及纤溶酶原激活物抑制物在子宫内膜的表达水平均升高(P<0.05)。与空白组和生理盐水组相比,第四周和第五周雌激素组和黄芩组子宫内膜腺体和间质较前萎缩,子宫内膜组织因子及纤溶酶原激活物抑制物在子宫内膜的表达水平降低(P<0.05)。
结论：单一雌激素全周期持续作用的青春期大鼠子宫内膜持续增生,在单味中药黄芩的作用下,大鼠子宫内膜组织中组织因子表达增加,可以激活组织因子,促使血栓形成和促凝活性增强而止血。纤溶酶原激活物抑制物在大鼠子宫内膜组织中的表达增加,使血管脆性和破坏降低,子宫内膜稳定性增强而止血。这可能是黄芩治疗青春期功血的止血机制之一。
Objective:investigate the continuing role of the cycle of the influence of radix scutellariae for the rats endometrial TF and PAI-1with no ovulation type dysfunctional uterine bleeding, and in order to investigate the hemostatic mechanism of radix scutellariae to endometrial hyperplasia and endometrial stability and persistent bleeding related indicator research and local micro environment of no ovulation type dysfunctional uterine bleeding acted by single estrogen, and the TF, PAI1is one of the relevant index.
Method:Divide56rats into2groups; the blank group including20is filled by saline, while the estrogen intervention group including36is filled by bujiale in3weeks continually, the continuing role of the cycle. Then kill4rats in each group weekly, and take bilateral uterine (with4%polyphosphate formaldehyde liquid fixed),keeping at low temperature. At the forth week,divide the estrogen intervention group into the negative control group,the positive control group and the drug control group, which of only8rats per group. Feed the negative control group by saline, feed the positive control group by estrogen and feed the drug control group by the liquid of radix scutellariae. Kill4rats in each group weekly, and take bilateral uterine (with4%polyphosphate formaldehyde liquid fixed),keeping at low temperature (the calculation of the dosage in accordance with the normal human body surface area in each group). Observe the endometrial morphological changes in each group's rat weekly by light mirror. Detect the condition of expression in the rat endometrial TF and PAI-1through the immunohistochemical methods.
Result:The experimental results show that comparing with the blank group,the endometrial glands and interstitial increase in each group weekly at the first3weeks, while the expression level of endometrial TF and PAI-1increase mildly (P<0.05); comparing with the blank group and negative control group, the endometrial glands and interstitial of the positive and drug control group atrophy in the forth and fifth week, while the expression level of endometrial TF and PAI-1decrease obviously (P<0.05)
Conclusion:Adolescence rats acted by single estrogen of the continuing role of the cycle appeared retreat hemorrhages, by the action of the only herb radix scutellariae, the increase of TF at the rats endometrial tissues can activate TF,making thrombosis and strengthening the activity to hemostatic; the increase of PAI-lat the rats endometrial tissues can restrain fibrinolysis syetem to hemostatic. That's maybe one of the mechanisms for radix scutellariae to treat the no ovulation type dysfunctional uterine bleeding.
方法：将56只大鼠随机分为2组,空白组20只灌胃生理盐水,模型组36只灌胃补佳乐,连续灌胃3周,即全周期雌激素干预。其中每组每周处死4只大鼠,取双侧子宫(用4%的多聚甲醛液固定),低温保存。第4周开始将模型组分为生理盐水组8只,雌激素组8只,黄芩组8只。生理盐水组灌胃生理盐水,雌激素组灌胃补佳乐,黄芩组灌胃黄芩悬液。每周各组处死4只大鼠,取双侧子宫(用4%的多聚甲醛液固定),低温保存。各组灌胃剂量按照正常人体体表面积比值计算。通过光镜观察每周各组大鼠子宫内膜形态学的变化。通过免疫组化法检测每周各组大鼠子宫内膜组织因子及纤溶酶原激活物抑制物在子宫内膜的表达情况。
结果：实验结果显示：与空白组相比,模型组前三周每周子宫内膜腺体和间质增生,子宫内膜组织因子及纤溶酶原激活物抑制物在子宫内膜的表达水平均升高(P<0.05)。与空白组和生理盐水组相比,第四周和第五周雌激素组和黄芩组子宫内膜腺体和间质较前萎缩,子宫内膜组织因子及纤溶酶原激活物抑制物在子宫内膜的表达水平降低(P<0.05)。
结论：单一雌激素全周期持续作用的青春期大鼠子宫内膜持续增生,在单味中药黄芩的作用下,大鼠子宫内膜组织中组织因子表达增加,可以激活组织因子,促使血栓形成和促凝活性增强而止血。纤溶酶原激活物抑制物在大鼠子宫内膜组织中的表达增加,使血管脆性和破坏降低,子宫内膜稳定性增强而止血。这可能是黄芩治疗青春期功血的止血机制之一。
Objective:investigate the continuing role of the cycle of the influence of radix scutellariae for the rats endometrial TF and PAI-1with no ovulation type dysfunctional uterine bleeding, and in order to investigate the hemostatic mechanism of radix scutellariae to endometrial hyperplasia and endometrial stability and persistent bleeding related indicator research and local micro environment of no ovulation type dysfunctional uterine bleeding acted by single estrogen, and the TF, PAI1is one of the relevant index.
Method:Divide56rats into2groups; the blank group including20is filled by saline, while the estrogen intervention group including36is filled by bujiale in3weeks continually, the continuing role of the cycle. Then kill4rats in each group weekly, and take bilateral uterine (with4%polyphosphate formaldehyde liquid fixed),keeping at low temperature. At the forth week,divide the estrogen intervention group into the negative control group,the positive control group and the drug control group, which of only8rats per group. Feed the negative control group by saline, feed the positive control group by estrogen and feed the drug control group by the liquid of radix scutellariae. Kill4rats in each group weekly, and take bilateral uterine (with4%polyphosphate formaldehyde liquid fixed),keeping at low temperature (the calculation of the dosage in accordance with the normal human body surface area in each group). Observe the endometrial morphological changes in each group's rat weekly by light mirror. Detect the condition of expression in the rat endometrial TF and PAI-1through the immunohistochemical methods.
Result:The experimental results show that comparing with the blank group,the endometrial glands and interstitial increase in each group weekly at the first3weeks, while the expression level of endometrial TF and PAI-1increase mildly (P<0.05); comparing with the blank group and negative control group, the endometrial glands and interstitial of the positive and drug control group atrophy in the forth and fifth week, while the expression level of endometrial TF and PAI-1decrease obviously (P<0.05)
Conclusion:Adolescence rats acted by single estrogen of the continuing role of the cycle appeared retreat hemorrhages, by the action of the only herb radix scutellariae, the increase of TF at the rats endometrial tissues can activate TF,making thrombosis and strengthening the activity to hemostatic; the increase of PAI-lat the rats endometrial tissues can restrain fibrinolysis syetem to hemostatic. That's maybe one of the mechanisms for radix scutellariae to treat the no ovulation type dysfunctional uterine bleeding.
引文
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