小麦1B·1R易位系与秆锈病抗性基因的分子检测
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摘要
1BL·1RS易位系在我国小麦育种和生产中占有重要地位,快速准确地鉴定1BL·1RS易位系对小麦改良具有重要意义。本研究选用15个1BL·1RS特异性STS、SCAR和RAPD标记及22个1RS染色体上的SSR标记,检测不同来源的1BL·1RS易位系78份以及非1BL·1RS易位系品种10份和黑麦材料3份,其中1BL·1RS易位系包括周8425B及其衍生系36份、兰考906及其衍生系5份、矮孟牛及其衍生系8份和洛类1BL·1RS易位系品种29份;利用10个抗秆锈病基因特异性STS、SCAR和SSR标记,检测308份秆锈病材料,其中包括32份CIMMYT抗秆锈病品种和276份中国抗秆锈病品种。主要结果如下:
1. 7个STS标记、1个SCAR标记、3个RAPD标记和3个黑麦SSR标记可作为鉴别1BL·1RS易位系的可靠分子标记,其中ω-sec-p1/ω-sec-p2、ω-sec-p3/ω-sec-p4、H20和SECA2/SECA3标记较好,扩增效果稳定,重复性好,条带清晰,实验操作简单。
2.周8425B及其衍生系、兰考906及其衍生系、矮孟牛及其衍生系与洛类1BL·1RS易位系的1RS染色体在分子标记检测中没有差异。
3.标记AF1/AF4和Iag95可作为初步检测抗秆锈病基因Sr31的分子标记,标记Gb可作为初步检测抗秆锈病基因Sr25的分子标记,标记gwm389、gwm533和barc133可作为初步检测抗秆锈病基因Sr2的分子标记,其中AF1/AF4、Gb、Iag95、gwm533标记较好,扩增效果稳定,重复性好,条带清晰,实验操作简单。
4. 308份小麦抗秆锈病材料中67份品种含抗秆锈病基因Sr31,12份品种含抗秆锈病基因Sr25,175份品种含抗秆锈病基因Sr2。
1BL·1RS translocation lines played an important role in wheat breeding and production in China. Rapid and accurate identification of 1BL·1RS translocation is important for wheat improvement. In order to discriminate the 1BL·1RS translocations from different origins, 10 STS, one SCAR and four RAPD markers specific to 1BL·1RS translocations as well as 22 SSR markers located on chromosome 1RS were employed to test 78 accessions with 1BL·1RS translocations, 10 non-1BL·1RS translocations, and three rye lines. The 1BL·1RS translocation lines included Zhou 8425B and its 36 derivatives, Lankao 906 and its five derivatives, Aimengniu and its eight derivatives, and Lovrin series with 1BL·1RS translocation (29 accessions). Four STS, one SCAR and five SSR markers for different stem rust resistance genes were employed to test resistance genes in 308 wheat genotypes accessions, including 32 CIMMYT lines and 276 Chinese materials. The results are summarized below.
1. Seven STS, one SCAR, three RAPD, and three SSR markers can be effectively used to identify the 1BL·1RS translocations. Among them, the markersω-sec-p1/ω-sec-p2,ω-sec-p3/ω-sec-p4, H20, and SECA2/SECA3 were better, with stable amplification, clear bands on electrophoresis gel, good repeatability, and simple operation.
2. In the 78 lines with 1BL·1RS translocations, no genetic diversities were observed in the 1RS translocations from different origins in the test with molecular markers.
3. AF1/AF4 and Iag95 markers can be used to identify originally the stem rust resistance gene Sr31, Gb marker can be used to identify originally the stem rust resistance gene Sr25, gwm389, gwm533 and barc133 can be used to identify originally the stem rust resistance gene Sr2. Of them, the markers AF1/AF4, Iag95, Gb, and gwm 533 were better, with stable amplification, clear bands on electrophoresis gel, good repeatability, and simple operation.
4. Among 308 wheat accession, Sr31 is present in 67 varieties, Sr25 is present in 12 varieties, and Sr2 is present in 175 varieties.
1. 7个STS标记、1个SCAR标记、3个RAPD标记和3个黑麦SSR标记可作为鉴别1BL·1RS易位系的可靠分子标记,其中ω-sec-p1/ω-sec-p2、ω-sec-p3/ω-sec-p4、H20和SECA2/SECA3标记较好,扩增效果稳定,重复性好,条带清晰,实验操作简单。
2.周8425B及其衍生系、兰考906及其衍生系、矮孟牛及其衍生系与洛类1BL·1RS易位系的1RS染色体在分子标记检测中没有差异。
3.标记AF1/AF4和Iag95可作为初步检测抗秆锈病基因Sr31的分子标记,标记Gb可作为初步检测抗秆锈病基因Sr25的分子标记,标记gwm389、gwm533和barc133可作为初步检测抗秆锈病基因Sr2的分子标记,其中AF1/AF4、Gb、Iag95、gwm533标记较好,扩增效果稳定,重复性好,条带清晰,实验操作简单。
4. 308份小麦抗秆锈病材料中67份品种含抗秆锈病基因Sr31,12份品种含抗秆锈病基因Sr25,175份品种含抗秆锈病基因Sr2。
1BL·1RS translocation lines played an important role in wheat breeding and production in China. Rapid and accurate identification of 1BL·1RS translocation is important for wheat improvement. In order to discriminate the 1BL·1RS translocations from different origins, 10 STS, one SCAR and four RAPD markers specific to 1BL·1RS translocations as well as 22 SSR markers located on chromosome 1RS were employed to test 78 accessions with 1BL·1RS translocations, 10 non-1BL·1RS translocations, and three rye lines. The 1BL·1RS translocation lines included Zhou 8425B and its 36 derivatives, Lankao 906 and its five derivatives, Aimengniu and its eight derivatives, and Lovrin series with 1BL·1RS translocation (29 accessions). Four STS, one SCAR and five SSR markers for different stem rust resistance genes were employed to test resistance genes in 308 wheat genotypes accessions, including 32 CIMMYT lines and 276 Chinese materials. The results are summarized below.
1. Seven STS, one SCAR, three RAPD, and three SSR markers can be effectively used to identify the 1BL·1RS translocations. Among them, the markersω-sec-p1/ω-sec-p2,ω-sec-p3/ω-sec-p4, H20, and SECA2/SECA3 were better, with stable amplification, clear bands on electrophoresis gel, good repeatability, and simple operation.
2. In the 78 lines with 1BL·1RS translocations, no genetic diversities were observed in the 1RS translocations from different origins in the test with molecular markers.
3. AF1/AF4 and Iag95 markers can be used to identify originally the stem rust resistance gene Sr31, Gb marker can be used to identify originally the stem rust resistance gene Sr25, gwm389, gwm533 and barc133 can be used to identify originally the stem rust resistance gene Sr2. Of them, the markers AF1/AF4, Iag95, Gb, and gwm 533 were better, with stable amplification, clear bands on electrophoresis gel, good repeatability, and simple operation.
4. Among 308 wheat accession, Sr31 is present in 67 varieties, Sr25 is present in 12 varieties, and Sr2 is present in 175 varieties.
引文
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