羟基磷灰石纳米材料的制备与表征
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摘要
人体的骨骼和牙齿的主要成分与羟基磷灰石十分相似,因而引起了研究者的广泛关注。随着羟基磷灰石的成功合成,人们也逐渐发现了它具有良好的生物相容性和生物活性,可以引导骨的生长,并可与骨组织形成牢固的骨性结合,因而被公认为性能良好的骨修复替代材料。纳米材料具有尺寸小、表面积大等诸多特点,决定了其具有优异的性能。在羟基磷灰石中掺杂稀土发光离子,可以起到生物示踪的作用。因而制备出羟基磷灰石纳米材料是一个具有生物应用前景的重要课题。
本文以CaCO3、NH4H2PO4为原料,CTAB为模板,利用水热法在不同温度下制备了羟基磷灰石纳米棒；以CTAB和EDTA为双模板,利用水热法制备了羟基磷灰石纳米棒；以EDTA为模板制备了铕、铽掺杂羟基磷灰石。同时,以Ca(CH3COO)2·H2O、H3PO4、PVP、DMF为主要原料,利用静电纺丝法制备了棒状羟基磷灰石及铕、铽掺杂羟基磷灰石多孔薄膜。采用XRD、FESEM、FTIR、UV-Vis,荧光光谱等分析技术对样品进行了系统表征。结果表明,所制备的铕、铽掺杂羟基磷灰石纳米棒都具有较好的发光性质,而且无生物毒性,可以在生物体内起到生物示踪作用,获得了一些有意义的结果,为羟基磷灰石纳米材料的进一步深入研究奠定了基础。
The main ingredients of skeleton and teeth of human are similar with chemical composition of hydroxyapatite, and therefore, researchers pay more attention to it. With the successful synthesis of hydroxyapatite, people also realize that it has better biocompatibility and bioactivity, which leads bone to grow up. It can combine with bones and therefore it is publicly called as good substitute materials for bone modification. Nanomaterials have the characteristics of small size and big surface area, which insure its better properties. If rare earth ions are incorporated into hydroxyapatite, it will play an important part in biological tracer. Thence, it will be an important research subject of good biological application to fabricate hydroxyapatite nanomaterials.
In this dissertation, at the different temperature conditions, using CaCO3 and NH4H2PO4 as starting materials, hydroxyapatite nanorods were prepared via hydrothermal method using CTAB as a template. Hydroxyapatite naonorods were prepared via hydrothermal method using both CTAB and EDTA as templates. Europium-doped and terbium-doped hydroxyapatite nanorods were prepared via hydrothermal method using EDTA as a template. In addition, rod-shaped hydroxyapatite. Europium-doped and terbium-doped hydroxyapatite thin membranes were prepared by electrospinning using Ca(CH3COO)2·H2O, H3PO4, PVP and DMF as main starting materials. The samples were systematically characterized by XRD, FESEM. EDS, FTIR, Fluorescence, etc. The results show that Europium-doped and terbium-doped hydroxyapatite naonorods have better fluorescence properties without any biological toxicity, which can be used as biological tracer. Some significant results were achieved, and solid foundations were built for the future study of hydroxyapatite nanomaterials.
本文以CaCO3、NH4H2PO4为原料,CTAB为模板,利用水热法在不同温度下制备了羟基磷灰石纳米棒；以CTAB和EDTA为双模板,利用水热法制备了羟基磷灰石纳米棒；以EDTA为模板制备了铕、铽掺杂羟基磷灰石。同时,以Ca(CH3COO)2·H2O、H3PO4、PVP、DMF为主要原料,利用静电纺丝法制备了棒状羟基磷灰石及铕、铽掺杂羟基磷灰石多孔薄膜。采用XRD、FESEM、FTIR、UV-Vis,荧光光谱等分析技术对样品进行了系统表征。结果表明,所制备的铕、铽掺杂羟基磷灰石纳米棒都具有较好的发光性质,而且无生物毒性,可以在生物体内起到生物示踪作用,获得了一些有意义的结果,为羟基磷灰石纳米材料的进一步深入研究奠定了基础。
The main ingredients of skeleton and teeth of human are similar with chemical composition of hydroxyapatite, and therefore, researchers pay more attention to it. With the successful synthesis of hydroxyapatite, people also realize that it has better biocompatibility and bioactivity, which leads bone to grow up. It can combine with bones and therefore it is publicly called as good substitute materials for bone modification. Nanomaterials have the characteristics of small size and big surface area, which insure its better properties. If rare earth ions are incorporated into hydroxyapatite, it will play an important part in biological tracer. Thence, it will be an important research subject of good biological application to fabricate hydroxyapatite nanomaterials.
In this dissertation, at the different temperature conditions, using CaCO3 and NH4H2PO4 as starting materials, hydroxyapatite nanorods were prepared via hydrothermal method using CTAB as a template. Hydroxyapatite naonorods were prepared via hydrothermal method using both CTAB and EDTA as templates. Europium-doped and terbium-doped hydroxyapatite nanorods were prepared via hydrothermal method using EDTA as a template. In addition, rod-shaped hydroxyapatite. Europium-doped and terbium-doped hydroxyapatite thin membranes were prepared by electrospinning using Ca(CH3COO)2·H2O, H3PO4, PVP and DMF as main starting materials. The samples were systematically characterized by XRD, FESEM. EDS, FTIR, Fluorescence, etc. The results show that Europium-doped and terbium-doped hydroxyapatite naonorods have better fluorescence properties without any biological toxicity, which can be used as biological tracer. Some significant results were achieved, and solid foundations were built for the future study of hydroxyapatite nanomaterials.
引文
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