富钒决明子对淋巴性脑水肿大鼠海马神经元的保护作用
摘要
目的
应用富钒决明子以普通决明子及无机钒(NaVO3)为对照,干预淋巴性脑水肿(lymphatic brain edema, LBE)大鼠,以评价富钒决明子对于淋巴性脑水肿大鼠海马神经元的保护作用,并探讨其可能机制；比较普通决明子、无机钒(NaVO3)与富钒决明子之间对于LBE大鼠海马神经元保护作用的差别。
方法
将大鼠随机分为5组：Sham组、LBE组、SC组、V组和V+SC组(n=10),Sham组、LBE组、V组均以普通大鼠饲料喂养,SC组、V+SC组分别应用普通决明子及富钒决明子大鼠饲料干预。Sham组行假手术,其余各组进行LBE手术。V组手术前后腹腔注射无机钒(NaV03)。应用Morris水迷宫检测大鼠空间学习记忆能力、尼氏染色检测大鼠海马CA1区椎体细胞层神经元功能状态、ChAT免疫组化染色和RT-PCR检测大鼠海马胆碱能神经元细胞活性,TUNEL染色检测大鼠海马CA1区椎体细胞层神经元凋亡。
结果
(1)各组大鼠每日进食饲料重量(60g/只/d)和每周体重增长重量(20g/w)无差异(P>0.05)；SC组与V+SC组大鼠每日饮水50ml,较Sham组(40m1/d)多(P<0.05)。(2)MWM定位航行试验中,V+SC组大鼠逃避潜伏期与Sham组大致相同,SC组、V组依次延长,LBE组大鼠逃避潜伏期最长,且始终高于其他组。(3)M WM空间搜索试验中,V+SC组大鼠与Sham组穿越平台次数、目标象限活动时间和目标象限路程/总路程三个指标均相近(P>0.05)。V+SC组、V组三个指标均明显高于LBE组(P<0.0])；SC组与LBE组无差异(P=0.131)。V+SC组明显高于V组(P<0.01)。(4)LBE组、V+SC组、V组、SC组、LBE组尼氏染色海马CA1区椎体细胞层神经元细胞深蓝、紫色区域的累积光密度(integrated optical density,IOD)值依次降低,各处理组与Sham组间以及各处理组间IOD值均有显著差异(P<0.01)。(5)LBE组、V+SC组、V组、SC组、LBE组ChAT免疫组化染色海马CA1区椎体细胞层神经元细胞黄色棕黄色区域IOD值依次降低,各处理组与Sham组间以及各处理组间IOD值均有显著差异(P<0.01)。(6)TUNEL染色海马CA1区椎体细胞层神经元细胞凋亡率,V+SC组与Sham组间无差别(P=0.338),其余各处理组与Sham组间以及各处理组间均有显著差异(P<0.01)。(7) RT-PCR中,ChAT表达量LBE组、V+SC组、V组、SC组、LBE组依次降低,各处理组与Sham组间以及各处理组间ChAT与β-actin灰度值之比均有显著差异(P<0.01)。
结论
(1)富钒决明子饲养的大鼠,其正常生长发育未受影响。(2)富钒决明子可以改善LBE大鼠的空间学习记忆能力,其可能机制是通过增加海马CA1区锥体细胞层神经元数目、提高胆碱神经元功能、抑制神经元凋亡。为LBE的治疗提供实验依据。(3)富钒决明子的神经保护作用优于普通决明子以及无机钒(NaVO3)。
Objective
We use common semen cassiae, inorganic vanadium(NaVO3) and semen cassiae enriching vanadium intervene lymphatic brain edema rats, detect related parameters, to evaluate the neuroprotective effects of semen cassiae enriching vanadium on neuron in lymphatic brain edema rats and to explore the mechanism of the neuroprotective effects. Compare the the difference in the neuroprotective effects of common semen cassiae, inorganic vanadium(NaV03) and semen cassiae enriching vanadium. Our destination is to provid a scientific guid and thought to the exploitation of semen cassiae and vanadium and the treatment of lymphatic brain edema.
Methods
The rats was divided into five groups randomly:group Sham,group LBE,group SC,group V and groupV+SC(n=10).The group Sham, group LBE and group V rats were rised by common fodder of rat, however,The group SC rats were feed by common fodder of rat with common semen cassiae,and The group V+SC rats were feed by common fodder of rat with semen cassiae enriching vanadium.All groups were taken the LBE operation but group Sham.Group V rats were injected inorganic vanadium(NaVO3) dissolved in physiological saline before and after the operation.The Morris Warter Maze was used to detect spatial learning and memory of the rats. Nissl's staining was to detect the function of neurons in CA1 region of hippocampus. Immunohistochemical staining of choline acetyltransferas and RT-PCR can detect the activity of acetyltransferas in the hippocampus. Detecting the apoptosis of the neurons in CA1 region of hippocampus by TUNEL staining.
Results
(1)In every groups, one rat eats 60g fodder every day and gains 20g body weight in a week, there is no difference between groups(P>0.05).Group SC and group V+SC taken more water(50ml/d of one rat) than the group Sham(40ml/d of one rat) (P<0.05).(2)In place navigation test of MWM, the escape latent period of group V+SC rats are identical to group Sham. The escape latent period of group SC and group V are prolong in turn. The escape latent period of group LBE is the most long and is longer than other groups always.(3)In spatial probe test of MWM,the group V+SC and group Sham are dentical in the index of number of crossing platform, time spent in the target quadrant and distance in the target quadrant/total distance(P>0.05).The three index of group SC and group V are significant higher than group LBE(P<0.05). there is not significantly different between group SC and group LBE(P=0.131).Group V+SC is higher than group V significantly(P<0.01).(4)In Nissl's staining, the IOD of dark blue and violet region in CA1 of hippocampus are reduced in order of group LBE, group V+SC, group V, group SC and group LBE.The differences between treatment groups and the differences between treatment groups and group Sham are significant(P<0.01).(5) Immunohistochemical staining show that the IOD of yellow and buffy region in CA1 of hippocampus are reduced in order of group LBE, group V+SC, group V, group SC and group LBE.The differences between treatment groups and the differences between treatment groups and group Sham are significant(P<0.01).(6)In TUNEL staining,there is no significant difference between group V+SC and group Sham in the ratio of apoptosis in CA1 of hippocampus(P=0.338).But the differences between other treatment groups and the differences between other treatment groups and group Sham are significant(P<0.01).(7)RT-PCR show that the expression of ChAT are reduced in order of group LBE, group V+SC, group V, group SC and group LBE.The differences between treatment groups and the differences between treatment groups and group Sham of ChAT/β-actin gray scale value are significant (P<0.01).
Conclusions
(1)The growing development of rats feed by common fodder of with semen cassiae enriching vanadium is not influenced.(2)The semen cassiae enriching vanadium can improve the spatial learning and memory of the LBE rats,and the mechanisms may be through increasing the number of neurons, elevating the function of cholinergic neurons and inhibiting the apoptosis of neurons in CA1 region of hippocampus.It can provid a new idear for the therapy of LBE.(3)The neuroprotective effects of the semen cassiae enriching vanadium is more effective than common semen cassiae and inorganic vanadium(NaV03).
应用富钒决明子以普通决明子及无机钒(NaVO3)为对照,干预淋巴性脑水肿(lymphatic brain edema, LBE)大鼠,以评价富钒决明子对于淋巴性脑水肿大鼠海马神经元的保护作用,并探讨其可能机制；比较普通决明子、无机钒(NaVO3)与富钒决明子之间对于LBE大鼠海马神经元保护作用的差别。
方法
将大鼠随机分为5组：Sham组、LBE组、SC组、V组和V+SC组(n=10),Sham组、LBE组、V组均以普通大鼠饲料喂养,SC组、V+SC组分别应用普通决明子及富钒决明子大鼠饲料干预。Sham组行假手术,其余各组进行LBE手术。V组手术前后腹腔注射无机钒(NaV03)。应用Morris水迷宫检测大鼠空间学习记忆能力、尼氏染色检测大鼠海马CA1区椎体细胞层神经元功能状态、ChAT免疫组化染色和RT-PCR检测大鼠海马胆碱能神经元细胞活性,TUNEL染色检测大鼠海马CA1区椎体细胞层神经元凋亡。
结果
(1)各组大鼠每日进食饲料重量(60g/只/d)和每周体重增长重量(20g/w)无差异(P>0.05)；SC组与V+SC组大鼠每日饮水50ml,较Sham组(40m1/d)多(P<0.05)。(2)MWM定位航行试验中,V+SC组大鼠逃避潜伏期与Sham组大致相同,SC组、V组依次延长,LBE组大鼠逃避潜伏期最长,且始终高于其他组。(3)M WM空间搜索试验中,V+SC组大鼠与Sham组穿越平台次数、目标象限活动时间和目标象限路程/总路程三个指标均相近(P>0.05)。V+SC组、V组三个指标均明显高于LBE组(P<0.0])；SC组与LBE组无差异(P=0.131)。V+SC组明显高于V组(P<0.01)。(4)LBE组、V+SC组、V组、SC组、LBE组尼氏染色海马CA1区椎体细胞层神经元细胞深蓝、紫色区域的累积光密度(integrated optical density,IOD)值依次降低,各处理组与Sham组间以及各处理组间IOD值均有显著差异(P<0.01)。(5)LBE组、V+SC组、V组、SC组、LBE组ChAT免疫组化染色海马CA1区椎体细胞层神经元细胞黄色棕黄色区域IOD值依次降低,各处理组与Sham组间以及各处理组间IOD值均有显著差异(P<0.01)。(6)TUNEL染色海马CA1区椎体细胞层神经元细胞凋亡率,V+SC组与Sham组间无差别(P=0.338),其余各处理组与Sham组间以及各处理组间均有显著差异(P<0.01)。(7) RT-PCR中,ChAT表达量LBE组、V+SC组、V组、SC组、LBE组依次降低,各处理组与Sham组间以及各处理组间ChAT与β-actin灰度值之比均有显著差异(P<0.01)。
结论
(1)富钒决明子饲养的大鼠,其正常生长发育未受影响。(2)富钒决明子可以改善LBE大鼠的空间学习记忆能力,其可能机制是通过增加海马CA1区锥体细胞层神经元数目、提高胆碱神经元功能、抑制神经元凋亡。为LBE的治疗提供实验依据。(3)富钒决明子的神经保护作用优于普通决明子以及无机钒(NaVO3)。
Objective
We use common semen cassiae, inorganic vanadium(NaVO3) and semen cassiae enriching vanadium intervene lymphatic brain edema rats, detect related parameters, to evaluate the neuroprotective effects of semen cassiae enriching vanadium on neuron in lymphatic brain edema rats and to explore the mechanism of the neuroprotective effects. Compare the the difference in the neuroprotective effects of common semen cassiae, inorganic vanadium(NaV03) and semen cassiae enriching vanadium. Our destination is to provid a scientific guid and thought to the exploitation of semen cassiae and vanadium and the treatment of lymphatic brain edema.
Methods
The rats was divided into five groups randomly:group Sham,group LBE,group SC,group V and groupV+SC(n=10).The group Sham, group LBE and group V rats were rised by common fodder of rat, however,The group SC rats were feed by common fodder of rat with common semen cassiae,and The group V+SC rats were feed by common fodder of rat with semen cassiae enriching vanadium.All groups were taken the LBE operation but group Sham.Group V rats were injected inorganic vanadium(NaVO3) dissolved in physiological saline before and after the operation.The Morris Warter Maze was used to detect spatial learning and memory of the rats. Nissl's staining was to detect the function of neurons in CA1 region of hippocampus. Immunohistochemical staining of choline acetyltransferas and RT-PCR can detect the activity of acetyltransferas in the hippocampus. Detecting the apoptosis of the neurons in CA1 region of hippocampus by TUNEL staining.
Results
(1)In every groups, one rat eats 60g fodder every day and gains 20g body weight in a week, there is no difference between groups(P>0.05).Group SC and group V+SC taken more water(50ml/d of one rat) than the group Sham(40ml/d of one rat) (P<0.05).(2)In place navigation test of MWM, the escape latent period of group V+SC rats are identical to group Sham. The escape latent period of group SC and group V are prolong in turn. The escape latent period of group LBE is the most long and is longer than other groups always.(3)In spatial probe test of MWM,the group V+SC and group Sham are dentical in the index of number of crossing platform, time spent in the target quadrant and distance in the target quadrant/total distance(P>0.05).The three index of group SC and group V are significant higher than group LBE(P<0.05). there is not significantly different between group SC and group LBE(P=0.131).Group V+SC is higher than group V significantly(P<0.01).(4)In Nissl's staining, the IOD of dark blue and violet region in CA1 of hippocampus are reduced in order of group LBE, group V+SC, group V, group SC and group LBE.The differences between treatment groups and the differences between treatment groups and group Sham are significant(P<0.01).(5) Immunohistochemical staining show that the IOD of yellow and buffy region in CA1 of hippocampus are reduced in order of group LBE, group V+SC, group V, group SC and group LBE.The differences between treatment groups and the differences between treatment groups and group Sham are significant(P<0.01).(6)In TUNEL staining,there is no significant difference between group V+SC and group Sham in the ratio of apoptosis in CA1 of hippocampus(P=0.338).But the differences between other treatment groups and the differences between other treatment groups and group Sham are significant(P<0.01).(7)RT-PCR show that the expression of ChAT are reduced in order of group LBE, group V+SC, group V, group SC and group LBE.The differences between treatment groups and the differences between treatment groups and group Sham of ChAT/β-actin gray scale value are significant (P<0.01).
Conclusions
(1)The growing development of rats feed by common fodder of with semen cassiae enriching vanadium is not influenced.(2)The semen cassiae enriching vanadium can improve the spatial learning and memory of the LBE rats,and the mechanisms may be through increasing the number of neurons, elevating the function of cholinergic neurons and inhibiting the apoptosis of neurons in CA1 region of hippocampus.It can provid a new idear for the therapy of LBE.(3)The neuroprotective effects of the semen cassiae enriching vanadium is more effective than common semen cassiae and inorganic vanadium(NaV03).
引文
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