基于贵金属纳米簇的传感器研究
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摘要
贵金属纳米簇(NCs),如金纳米簇(AuNCs)、银纳米簇(AgNCs),是一类很有用的纳米材料。最近几年,高荧光强度,高稳定性的金属纳米簇被密集的报道作为荧光纳米簇探针,用于检测很多种类的目标物。大多数这些检测方法是基于金属纳米簇的荧光增敏或猝灭。在本论文中,我们以目标物对贵金属簇荧光的猝灭效应,建立了多种传感体系。
本论文第一部分实验中,我们合成了牛血清白蛋白(BSA)包裹的银纳米簇,研究了BSA-AgNCs荧光探针对金属离子的响应,以荧光猝灭的方式建立了一种检测Cu2+的方法。并利用组氨酸对铜离子荧光猝灭的抑制的效应,建立了间接检测组氨酸的传感器。
本论文第二部分实验我们建立了一种提高金纳米簇对Hg2+和Cu2+检测选择性的方法。BSA包裹的金纳米簇以荧光猝灭的方式对这两种离子产生响应。EDTA和NaBH4分别加入到相应的体系中作为掩蔽剂,EDTA能与Cu2+络合,NaBH4能把Hg2+还原为Hg0。这两种离子都能抑制相应的离子与纳米簇的相互作用,排除相应的猝灭效应,从而能够检测另一种离子。即使在这两种离子共存的情况下,纳米簇对这两种离子的相应也能有很好的选择性。
Noble metal nanocluster(NCs), such as gold and silver ones, are a type of useful nanomaterials. In recent years, highly luminescence and stable NCs are intensively reported as fluorescence probes for a wide range of targets. Most of these detections are based on either the enhancement or the queching of the fluorescence. In this paper, based on the fluorescence quenching from targets, we developed a variety of sensing systems.
In the first part, we synthesised silver nanoclusters stabibized by bovine serum albumin, and we studied the response of silver nanocluster fluorescent probe toward metal ions, developed a method to detect Cu2＋through fluorescence quenching. Furtherly, the complexation of histidine toward copper ion was adopted for an indirect detection of histidine.
In the second part, we developed a method that improved the selectivity of gold nanoclusters toward copper and mercury ions. Bovine serum albumin stabilized gold nanocluster responded to both these two ions through fluorescence quenching. EDTA and sodium borohydride were added respectively as the "masking" reagents, in which EDTA complexed with Cu2＋, and borohydride reduced Hg2＋into HgO. Both these reactions inhibited the ions'interaction with the nanocluster and eliminated related quenching effect, thus detection of the other ion was achieved. Good selectivity of the nanocluster over these two ions was obtained enabling sensitive detection even under their coexistence.
本论文第一部分实验中,我们合成了牛血清白蛋白(BSA)包裹的银纳米簇,研究了BSA-AgNCs荧光探针对金属离子的响应,以荧光猝灭的方式建立了一种检测Cu2+的方法。并利用组氨酸对铜离子荧光猝灭的抑制的效应,建立了间接检测组氨酸的传感器。
本论文第二部分实验我们建立了一种提高金纳米簇对Hg2+和Cu2+检测选择性的方法。BSA包裹的金纳米簇以荧光猝灭的方式对这两种离子产生响应。EDTA和NaBH4分别加入到相应的体系中作为掩蔽剂,EDTA能与Cu2+络合,NaBH4能把Hg2+还原为Hg0。这两种离子都能抑制相应的离子与纳米簇的相互作用,排除相应的猝灭效应,从而能够检测另一种离子。即使在这两种离子共存的情况下,纳米簇对这两种离子的相应也能有很好的选择性。
Noble metal nanocluster(NCs), such as gold and silver ones, are a type of useful nanomaterials. In recent years, highly luminescence and stable NCs are intensively reported as fluorescence probes for a wide range of targets. Most of these detections are based on either the enhancement or the queching of the fluorescence. In this paper, based on the fluorescence quenching from targets, we developed a variety of sensing systems.
In the first part, we synthesised silver nanoclusters stabibized by bovine serum albumin, and we studied the response of silver nanocluster fluorescent probe toward metal ions, developed a method to detect Cu2＋through fluorescence quenching. Furtherly, the complexation of histidine toward copper ion was adopted for an indirect detection of histidine.
In the second part, we developed a method that improved the selectivity of gold nanoclusters toward copper and mercury ions. Bovine serum albumin stabilized gold nanocluster responded to both these two ions through fluorescence quenching. EDTA and sodium borohydride were added respectively as the "masking" reagents, in which EDTA complexed with Cu2＋, and borohydride reduced Hg2＋into HgO. Both these reactions inhibited the ions'interaction with the nanocluster and eliminated related quenching effect, thus detection of the other ion was achieved. Good selectivity of the nanocluster over these two ions was obtained enabling sensitive detection even under their coexistence.
引文
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