多效唑对盾叶薯蓣皂素合成、活性氧代谢及膜脂过氧化反应的影响
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摘要
多效唑(paclobutrazol，PBZ)是一种新型、高效的植物生长调节剂，其应用非常广泛。本文以盾叶薯蓣为材料，研究了多效唑对盾叶薯蓣皂素合成、活性氧代谢及膜脂过氧化反应的影响，主要结果如下：
1．用25、50、100、200、400mg／L的多效唑处理盾叶薯蓣(Dioscorea zingiberensis Wright)植株，结果发现，不同浓度的多效唑处理均促进了盾叶薯蓣根状茎中薯蓣皂素(diosgenin)的合成和积累，其中100mg／L多效唑处理效果最好，薯蓣皂素含量比对照提高了56.5％。
2．进一步研究了不同浓度的多效唑(25、50、100、200、400mg／L)对正常生长条件下盾叶薯蓣叶片活性氧代谢和膜脂过氧化反应的影响，结果表明，不同浓度的多效唑均显著降低了盾叶薯蓣叶片中超氧物自由基(O_2~-．)产生速率、丙二醛(malondialdehyde，MDA)的含量，提高了超氧化物歧化酶(superoxide dismutase，SOD)、过氧化氢酶(catalase，CAT)、过氧化物酶(puaiacol peroxidase，POD)、抗坏血酸过氧化物酶(ascorbic acid peroxidase，APX)活性，促进了可溶性蛋白质和叶绿素的合成，其中100mg／L多效唑处理效果最明显。
3.干早胁迫下的盾叶薯祯叶片中02’.产生速率、MDA和游离脯氨酸含
量、电解质的相对外渗率均急剧上升，SOD、CAT、POD、妙X活性均不
同程度的显著下降，可溶性蛋白质和叶绿素的合成也受到了明显抑制。用
100m叭多效哇多效哇处理后，上述变化均有明显缓解，这说明多效哇处
理在一定程度上提高了盾叶薯裁植株的抗逆性。
Paclobutrazol (PBZ), which is a new and effective plant growth regulator, has been put into use very widely. This paper studied the effects of paclobutrazol on diosgenin synthesis, reactive oxygen metabolism and lipid peroxidation in Dioscorea zingiberensis Wright plants. The main results were as follows:
1. The effects of various concentrations (25, 50, 100, 200 and 400mg/L) of Paclobutrazol (PBZ) on the level of diosgenin in tubers of China endemic species Dioscorea zingiberensis Wright were observed. The results show that diosgenin synthesis was stimulated by PBZ, and the best result was in the case of 100mg/L treatment in which the diosgenin level was increased by 56.5% than control.
2. This paper further studied the effects of various concentrations of PBZ on reactive oxygen metabolism and lipid peroxidation in Dioscorea zingiberensis Wright leaves under normal growth condition, the results show that 25, 50, 100,
200, 400mg/L PBZ-treated Dioscorea zingiberensis Wright leaves were all decreased superoxide anion radical (O2-) production rate, stimulated an increment in the activities of superoxide dismutase (SOD), catalase (CAT), puaiacol peroxidase (POD), and ascorbic acid peroxidase (APX) activity, reduced malondialdehyde (MDA) content, and promoted the synthesis of soluble protein and chlorophyll. From the results, we can also see that 100 mg/L PBZ-treated is the best effective than the others.
3. Drought brought about over production of reactive oxygen species (ROS) in Dioscorea zingiberensis Wright leaves, measured as an increment in O2-. production rate, MDA and free proline content and electrolytic relative exosmosis rate; an decrement in the activities of SOD, CAT, POD, APX activity and soluble protein and chlorophyll contents. However, after PBZ treatment, the changes above were maintained to a lower degree than their corresponding controls after exposure to drought stress, this indictated that PBZ-treated had elevated stress-resistance in Dioscorea zingiberensis Wright plants.
1．用25、50、100、200、400mg／L的多效唑处理盾叶薯蓣(Dioscorea zingiberensis Wright)植株，结果发现，不同浓度的多效唑处理均促进了盾叶薯蓣根状茎中薯蓣皂素(diosgenin)的合成和积累，其中100mg／L多效唑处理效果最好，薯蓣皂素含量比对照提高了56.5％。
2．进一步研究了不同浓度的多效唑(25、50、100、200、400mg／L)对正常生长条件下盾叶薯蓣叶片活性氧代谢和膜脂过氧化反应的影响，结果表明，不同浓度的多效唑均显著降低了盾叶薯蓣叶片中超氧物自由基(O_2~-．)产生速率、丙二醛(malondialdehyde，MDA)的含量，提高了超氧化物歧化酶(superoxide dismutase，SOD)、过氧化氢酶(catalase，CAT)、过氧化物酶(puaiacol peroxidase，POD)、抗坏血酸过氧化物酶(ascorbic acid peroxidase，APX)活性，促进了可溶性蛋白质和叶绿素的合成，其中100mg／L多效唑处理效果最明显。
3.干早胁迫下的盾叶薯祯叶片中02’.产生速率、MDA和游离脯氨酸含
量、电解质的相对外渗率均急剧上升，SOD、CAT、POD、妙X活性均不
同程度的显著下降，可溶性蛋白质和叶绿素的合成也受到了明显抑制。用
100m叭多效哇多效哇处理后，上述变化均有明显缓解，这说明多效哇处
理在一定程度上提高了盾叶薯裁植株的抗逆性。
Paclobutrazol (PBZ), which is a new and effective plant growth regulator, has been put into use very widely. This paper studied the effects of paclobutrazol on diosgenin synthesis, reactive oxygen metabolism and lipid peroxidation in Dioscorea zingiberensis Wright plants. The main results were as follows:
1. The effects of various concentrations (25, 50, 100, 200 and 400mg/L) of Paclobutrazol (PBZ) on the level of diosgenin in tubers of China endemic species Dioscorea zingiberensis Wright were observed. The results show that diosgenin synthesis was stimulated by PBZ, and the best result was in the case of 100mg/L treatment in which the diosgenin level was increased by 56.5% than control.
2. This paper further studied the effects of various concentrations of PBZ on reactive oxygen metabolism and lipid peroxidation in Dioscorea zingiberensis Wright leaves under normal growth condition, the results show that 25, 50, 100,
200, 400mg/L PBZ-treated Dioscorea zingiberensis Wright leaves were all decreased superoxide anion radical (O2-) production rate, stimulated an increment in the activities of superoxide dismutase (SOD), catalase (CAT), puaiacol peroxidase (POD), and ascorbic acid peroxidase (APX) activity, reduced malondialdehyde (MDA) content, and promoted the synthesis of soluble protein and chlorophyll. From the results, we can also see that 100 mg/L PBZ-treated is the best effective than the others.
3. Drought brought about over production of reactive oxygen species (ROS) in Dioscorea zingiberensis Wright leaves, measured as an increment in O2-. production rate, MDA and free proline content and electrolytic relative exosmosis rate; an decrement in the activities of SOD, CAT, POD, APX activity and soluble protein and chlorophyll contents. However, after PBZ treatment, the changes above were maintained to a lower degree than their corresponding controls after exposure to drought stress, this indictated that PBZ-treated had elevated stress-resistance in Dioscorea zingiberensis Wright plants.
引文
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