Ⅰ群禽腺病毒的分离鉴定和生物学特性研究及重组禽腺病毒的构建
|
摘要
腺病毒最早发现于1953年,由于它们倾向于感染上皮细胞而被命名为腺病毒。腺病毒属腺病毒科(Adenoviridae),共分为四个属即哺乳动物腺病毒属(Mastadenovirus)、禽腺病毒属(Aviadenovirus)、腺胸腺病毒属(Atadenovirus)和唾液腺病毒属(Siadenovirus)。禽腺病毒是禽类常见的病毒之一,大部分在禽体内复制却不致病,少数引起轻微的病症。对禽腺病毒的流行和变异国外有很多研究工作,国内的报道较少。
腺病毒作为载体,宿主范围广,致病性低,在机体内复制不发生整合,安全性高;能在增殖和非增殖细胞中感染和表达基因;能有效进行增殖,病毒滴度高;腺病毒载体应用方便,载体疫苗可经口服途径接种。因此以腺病毒作为病毒载体具有独特的优势。许多学者在禽类腺病毒载体方面做了很多研究工作,国内研究较晚,但是所研制的基因工程疫苗离临床应用还有一定距离。目前,对禽腺病毒载体研究主要使用CMV启动子,获得了较好的表达效果,也有使用病毒自身的晚期启动子/先导序列(MLP/LS)的报道,但是尚未见到对两个启动子的重组病毒的免疫效果进行比较的报道。
本研究从外表健康禽群中随机采取泄殖腔拭子,根据禽腺病毒CELOV的全序列设计引物,PCR扩增了病毒基因组右末端1.5Kbp的ITR片段。结果表明:禽腺病毒在禽群中的流行比较广泛,鸡群的阳性率为23.8%(30/126),鸭群的阳性率为51.7%(15/29),鹅群的阳性率为17.3%(10/58)。从鸡源、鸭源、鹅源ITR扩增阳性的样品中各选取3株,将ITR片段的PCR扩增产物克隆测序,并与CELOV、FAV-9、EDSV以及FAV-JS株的相应序列进行了比较。结果表明:本次分离的9株禽腺病毒与血清1型的代表株CELOV和我们实验室早期分离株FAV-JS株在ITR片段上的同源性最高可达99.9%,最低也为94.7%;而与D亚群的FAV-9和禽腺病毒Ⅲ群的EDSV相比较,同源性较低。在此基础上,根据已经发表的CELOV的全基因组序列设计了引物,对分离株的保守序列ORF8进行了扩增和序列测定工作,结果表明所有分离株、CELOV、FAV-9、EDSV以及FAV-JS株的ORF8片段均具有高度同源性,符合腺病毒的基因组结构特征。根据以上序列分析结果,本研究证明了这些分离到的禽腺病毒属于Ⅰ群禽腺病毒。同时,禽腺病毒在鸡群、鸭群、鹅群中非常普遍,有必要对这些病毒的生物学特性进行研究。
为了更好的了解分离株的生物学特性以便探讨其作为活病毒疫苗载体的可能性,通过病毒凝集实验、电镜形态观察、细胞病变、动物攻毒实验等方法对上述疑为Ⅰ群禽腺病毒3个分离株进行了研究。电镜观察病毒粒子,结果发现病毒为球型、无囊膜,直径为70-80nm,具有腺病毒典型的二十面体结构。红细胞凝集试验证明,分离到的病毒均不能凝集鸡的红细胞,与Ⅰ群禽腺病毒的特征一致。细胞培养表明:病毒分离株不能引起鸡胚成纤维细胞(CEF)的细胞病变,而在鸡胚肾细胞(CEK)上可以产生典型的禽腺病毒的细胞病变,表现为细胞变圆,折光性增强等病变。鸡胚接种试验未见明显肉眼可见的病变,鸡胚生长发育正常,这与Ⅰ群禽腺病毒分离株CELOV的特性不完全一致。
通过大剂量皮下注射和口服两种途径接种1日龄的SPF鸡,进行人工致病性试验。结果发现,试验鸡在感染病毒后,未出现任何明显临床症状。在10日龄时,皮下注射和口服攻毒的小鸡的体重都明显轻于对照组,而在20、30日龄时,各组和健康对照组之间体重没有明显差异,表明该分离株对SPF鸡的体重影响不大,呈一过性。在10日龄时,攻毒组小鸡主要免疫器官重量略低于对照组,实验后期各组免疫免疫器官的重量基本一致。组织病理学切片观察结果表明,部分脏器有轻微病变,大部分脏器基本没有病变,后期组织器官均正常。这表明该病毒对免疫器官没有明显的生长抑制和可见的病理损伤。以上体内外实验均表明病毒分离株致病性低,生物安全性高,可能是较好的禽腺病毒载体。
前期研究中筛选了FAV-JS的复制非必需区,将CMV调控外源基因表达的表达盒插入的禽腺病毒FAV-JS株左右臂构建了通用转移载体pFACMV3.1。在此研究基础上,从本实验室分离的所有禽腺病毒中选择FAV-JS进行重组禽腺病毒的构建。本研究根据CELOV全基因序列设计引物,从FAVI-JS基因组中PCR扩增出腺病毒的主要晚期启动子(MLP),将扩增产物克隆测序,并与CELOV的MLP进行了序列比较,两者同源性为99%。在此基础上,将FAVI-JS株MLP片段克隆至pcDNA3.1/zeo(+),然后再将pFACMV3.1中含有部分L片段和完整R片段的L#-R片段克隆至同一pcDNA3.1/zeo(+)中,随后将含有MLP片段、部分L片段和完整R片段的大片段克隆至pFACMV3.1,将pFACMV3.1中的CMV启动子替换为MLP启动子,而其它部分不变,从而构建了MLP启动子控制的通用转移载体pFAMLP 3.1。在此基础上插入eGFP报告基因,构建了MLP启动子控制表达eGFP的正向转移载体pFAMLP-eGFP,以用于重组病毒的构建。
将构建的pFAMLP-eGFP转移载体转染原代鸡胚肾细胞(CEK)细胞,证实所克隆的MLP启动子具有启动子活性。在此研究基础上,将pFAMLP-eGFP转染已被wtFAVI-JS分离株感染的原代鸡胚肾细胞(CEK),进行细胞内同源重组。转染后的细胞及细胞上清混合物经过冻融和离心处理,取上清进行稀释,感染96孔细胞培养板上的CEK单层,结果连续传了三代后,仍然能观察到绿色荧光蛋白的表达,表明成功构建了表达eGFP的重组禽腺病毒,为探讨晚期启动子在重组病毒中是否可以高效的表达外源蛋白,重组禽腺病毒基因工程疫苗的启动子对疫苗免疫效果的影响提供了条件。
Adenovirus was found in 1949, which is tendency to infect epithelial cell. Adenoviridae is divided into two genuses, the Mastadenoviridae and the Aviadenoviridae in the past. However, Atadenoviridae and Siadenoviridae were added to this family in the ICTV 8th report, 2005. Duck adenovirus A is classified as members of Atadenoviridae while HEV as Siadenoviridae, an intermediate between the Mastadenoviridae and the Aviadenoviridae because both encode sialidase in the right structure of the genome. It would be worth notice that EDSV is called Duck adenovirus A and Turkey adenovirus A is designated as new species after this report. Fowl adenoviruses are divided into three groups by differences in serotype, most of which only replicate in birds and don't cause clinical symptoms or only have low pathogenicity. But most of them will influence the health of birds if there are other inducing factors or they superinfect with other pathogens while some fowl adenoviruses themselves are pathogenic. There are some reports about the prevalence or variation of fowl adenovirus (FAV) in foreign countries, but little in China.
With the development of molecular biology, many viral vectors are being used for gene therapy, vaccination or transfer. Differently from retrovirus, adenovirus does not depend on host cell division for its replication, and its chromosome rarely integrates into the cell genome, remaining episomal in most cases. Adenoviral vectors have a broad spectrum of cell infectivity that includes virtually all post-mitotic and mitotic cells, and also can be produced in high titers. Therefore, it is convenient to use adenoviral vectors for gene therapy or transfer by many routs. It has been found that birds were reinfected with the same strain after 8 week, eliciting a secondary response neutralizing and precipitating antibodies; virus excretion also occurred despite of humoral antibody. So, if the virus can be constructed into a vector to express foreign protein, it would induce strong immunity against foreign protein in chickens for a long time. Up to now, there is some preliminary research on FAV vector in foreign countries, while there was little research progress in China. The CMV promoter is used more popularly than the major late promoter of FAV in the construct of recombinant virus, but which one is more efficient to express the foreign gene is unknown.
In this study, fowl adenoviruses (FAV) distributions in flocks of chickens, ducks and geese were investigated by PCR. The results show that FAV was extensively popular in chickens, geese and ducks, with the positive rate of 51.4%, 51.7%, and 17.3%, respectively. Three positive samples were selected separately from chickens, ducks and geese, and then the open reading frame 8 (ORF8) and the right terminal of viral genome (ITR), were amplified, sequenced and analyzed. All nine isolates were 94.7%-99.9% homologous to FAVI when compared with that of CELOV, FAVI-JS, but shared low nucleotide sequence homology to FAV-9 and EDSV. They all converged into the same lineage with CELOV, FAVI-JS, while the viruses from group III shared the other one. The sequence of the ORF8 from all the above fowl adenoviruses shared very high homology to each other, which is in accordance with the genomic structural feature of adenovirus. From these results, we can presume that the fowl adenoviruses isolates belong to FAV group I.
In order to investigate the possibility of FAV as live vaccine vector, the biological characterization of FAVs isolates were further studied with technology of electron microscope, virus morphology, cytopathogenic effect (CPE) and experiments in ovo and in vivo. The results of these experiments showed that the virus particles are spherical, non-enveloped, and about 70-80nm in diameter and couldn't agglutinate the erythrocytes of chickens. The virus couldn't cause CPE in chicken embryo fibroblast cells (CEFs), but cause typical CPE of FAV in chicken embryonic kidney cells (CEKs), which became round with stronger light refraction. No gross lesions and growth retardation was observed in chicken embryo inoculated with the isolates, which are not completely in conformity with the characterization of CELOV (Chicken embryo lethal orphan virus).
To evaluate the safety of isolated viruses, SPF chickens were inoculated with the FAVI-G24-0605 at 1-day-old by subcutaneous injection or oral way. The weight of body and some immune organs were measured when a couple of chicken was euthanized at 10d, 20d, and 30d post infection (p.i). The results showed that the inoculated chickens were all healthy with no gross lesions during the animal experiment and there were no serious effects on the body weight of SPF chickens, although the body weight of chickens was lower than the control at 10d p.i and became normal at 20d and 30d p.i. The same changes also occurred in the weight of major immune organs of chickens inoculated. Most of tissue samples had no histopathological changes except some minor lesions at the early stage. These results indicate that the FAVI-G24-0605 strain may be a good candidate for recombinant FAV.
The non-essential region for replication of FAVI-JS was determined and the plasmid pFACMV3.1, a universal transfer vector for gene transferred to FAVI-JS, has been constructed by our studying team. On the basis of this research, FAVI-JS was selected from all the fowl adenovirus isolated in our lab for recombination. The major late promoter (MLP) of FAVI-JS, which belongs to group I avian adenovirus, were amplified by PCR and the sequence of MLP has high homology (99%) with that of CELOV. Then the MLP, together with a fragment from pFACMV3.1, containing the R fragment and the large part of L fragment, were cloned into pcDNA3.1/zeo (+). The above large fragment, digested with another two restrict enzymes, was subcloned to pFACMV3.1 to produce pFAMLP3.1, an universal transfer vector controlled by the MLP. By the same way, the ORF of eGFP gene was cloned into pFAMLP3.1 to construct pFAMLP-eGFP as a transfer vector with eGFP controlled by the MLP.
CEK was transfected with pFAMLP-eGFP plasmid. Expression of eGFP was found at 48h post transfection. Then CEK infected with wt-FAVI-JS was transfected with pFAMLP-eGFP to produce recombinant virus. The transfected cells and supernatant was freezed, thawed and centrifuged after 3-4d. And the diluted supernatant was re-infected CEK. The expression of eGFP was observed for at least 3 passages. Thus the recombinant fowl adenovirus rFAMLP-eGFP was successfully constructed and provided the basis for study the influence of the promoter on the immune efficiency of FAV.
腺病毒作为载体,宿主范围广,致病性低,在机体内复制不发生整合,安全性高;能在增殖和非增殖细胞中感染和表达基因;能有效进行增殖,病毒滴度高;腺病毒载体应用方便,载体疫苗可经口服途径接种。因此以腺病毒作为病毒载体具有独特的优势。许多学者在禽类腺病毒载体方面做了很多研究工作,国内研究较晚,但是所研制的基因工程疫苗离临床应用还有一定距离。目前,对禽腺病毒载体研究主要使用CMV启动子,获得了较好的表达效果,也有使用病毒自身的晚期启动子/先导序列(MLP/LS)的报道,但是尚未见到对两个启动子的重组病毒的免疫效果进行比较的报道。
本研究从外表健康禽群中随机采取泄殖腔拭子,根据禽腺病毒CELOV的全序列设计引物,PCR扩增了病毒基因组右末端1.5Kbp的ITR片段。结果表明:禽腺病毒在禽群中的流行比较广泛,鸡群的阳性率为23.8%(30/126),鸭群的阳性率为51.7%(15/29),鹅群的阳性率为17.3%(10/58)。从鸡源、鸭源、鹅源ITR扩增阳性的样品中各选取3株,将ITR片段的PCR扩增产物克隆测序,并与CELOV、FAV-9、EDSV以及FAV-JS株的相应序列进行了比较。结果表明:本次分离的9株禽腺病毒与血清1型的代表株CELOV和我们实验室早期分离株FAV-JS株在ITR片段上的同源性最高可达99.9%,最低也为94.7%;而与D亚群的FAV-9和禽腺病毒Ⅲ群的EDSV相比较,同源性较低。在此基础上,根据已经发表的CELOV的全基因组序列设计了引物,对分离株的保守序列ORF8进行了扩增和序列测定工作,结果表明所有分离株、CELOV、FAV-9、EDSV以及FAV-JS株的ORF8片段均具有高度同源性,符合腺病毒的基因组结构特征。根据以上序列分析结果,本研究证明了这些分离到的禽腺病毒属于Ⅰ群禽腺病毒。同时,禽腺病毒在鸡群、鸭群、鹅群中非常普遍,有必要对这些病毒的生物学特性进行研究。
为了更好的了解分离株的生物学特性以便探讨其作为活病毒疫苗载体的可能性,通过病毒凝集实验、电镜形态观察、细胞病变、动物攻毒实验等方法对上述疑为Ⅰ群禽腺病毒3个分离株进行了研究。电镜观察病毒粒子,结果发现病毒为球型、无囊膜,直径为70-80nm,具有腺病毒典型的二十面体结构。红细胞凝集试验证明,分离到的病毒均不能凝集鸡的红细胞,与Ⅰ群禽腺病毒的特征一致。细胞培养表明:病毒分离株不能引起鸡胚成纤维细胞(CEF)的细胞病变,而在鸡胚肾细胞(CEK)上可以产生典型的禽腺病毒的细胞病变,表现为细胞变圆,折光性增强等病变。鸡胚接种试验未见明显肉眼可见的病变,鸡胚生长发育正常,这与Ⅰ群禽腺病毒分离株CELOV的特性不完全一致。
通过大剂量皮下注射和口服两种途径接种1日龄的SPF鸡,进行人工致病性试验。结果发现,试验鸡在感染病毒后,未出现任何明显临床症状。在10日龄时,皮下注射和口服攻毒的小鸡的体重都明显轻于对照组,而在20、30日龄时,各组和健康对照组之间体重没有明显差异,表明该分离株对SPF鸡的体重影响不大,呈一过性。在10日龄时,攻毒组小鸡主要免疫器官重量略低于对照组,实验后期各组免疫免疫器官的重量基本一致。组织病理学切片观察结果表明,部分脏器有轻微病变,大部分脏器基本没有病变,后期组织器官均正常。这表明该病毒对免疫器官没有明显的生长抑制和可见的病理损伤。以上体内外实验均表明病毒分离株致病性低,生物安全性高,可能是较好的禽腺病毒载体。
前期研究中筛选了FAV-JS的复制非必需区,将CMV调控外源基因表达的表达盒插入的禽腺病毒FAV-JS株左右臂构建了通用转移载体pFACMV3.1。在此研究基础上,从本实验室分离的所有禽腺病毒中选择FAV-JS进行重组禽腺病毒的构建。本研究根据CELOV全基因序列设计引物,从FAVI-JS基因组中PCR扩增出腺病毒的主要晚期启动子(MLP),将扩增产物克隆测序,并与CELOV的MLP进行了序列比较,两者同源性为99%。在此基础上,将FAVI-JS株MLP片段克隆至pcDNA3.1/zeo(+),然后再将pFACMV3.1中含有部分L片段和完整R片段的L#-R片段克隆至同一pcDNA3.1/zeo(+)中,随后将含有MLP片段、部分L片段和完整R片段的大片段克隆至pFACMV3.1,将pFACMV3.1中的CMV启动子替换为MLP启动子,而其它部分不变,从而构建了MLP启动子控制的通用转移载体pFAMLP 3.1。在此基础上插入eGFP报告基因,构建了MLP启动子控制表达eGFP的正向转移载体pFAMLP-eGFP,以用于重组病毒的构建。
将构建的pFAMLP-eGFP转移载体转染原代鸡胚肾细胞(CEK)细胞,证实所克隆的MLP启动子具有启动子活性。在此研究基础上,将pFAMLP-eGFP转染已被wtFAVI-JS分离株感染的原代鸡胚肾细胞(CEK),进行细胞内同源重组。转染后的细胞及细胞上清混合物经过冻融和离心处理,取上清进行稀释,感染96孔细胞培养板上的CEK单层,结果连续传了三代后,仍然能观察到绿色荧光蛋白的表达,表明成功构建了表达eGFP的重组禽腺病毒,为探讨晚期启动子在重组病毒中是否可以高效的表达外源蛋白,重组禽腺病毒基因工程疫苗的启动子对疫苗免疫效果的影响提供了条件。
Adenovirus was found in 1949, which is tendency to infect epithelial cell. Adenoviridae is divided into two genuses, the Mastadenoviridae and the Aviadenoviridae in the past. However, Atadenoviridae and Siadenoviridae were added to this family in the ICTV 8th report, 2005. Duck adenovirus A is classified as members of Atadenoviridae while HEV as Siadenoviridae, an intermediate between the Mastadenoviridae and the Aviadenoviridae because both encode sialidase in the right structure of the genome. It would be worth notice that EDSV is called Duck adenovirus A and Turkey adenovirus A is designated as new species after this report. Fowl adenoviruses are divided into three groups by differences in serotype, most of which only replicate in birds and don't cause clinical symptoms or only have low pathogenicity. But most of them will influence the health of birds if there are other inducing factors or they superinfect with other pathogens while some fowl adenoviruses themselves are pathogenic. There are some reports about the prevalence or variation of fowl adenovirus (FAV) in foreign countries, but little in China.
With the development of molecular biology, many viral vectors are being used for gene therapy, vaccination or transfer. Differently from retrovirus, adenovirus does not depend on host cell division for its replication, and its chromosome rarely integrates into the cell genome, remaining episomal in most cases. Adenoviral vectors have a broad spectrum of cell infectivity that includes virtually all post-mitotic and mitotic cells, and also can be produced in high titers. Therefore, it is convenient to use adenoviral vectors for gene therapy or transfer by many routs. It has been found that birds were reinfected with the same strain after 8 week, eliciting a secondary response neutralizing and precipitating antibodies; virus excretion also occurred despite of humoral antibody. So, if the virus can be constructed into a vector to express foreign protein, it would induce strong immunity against foreign protein in chickens for a long time. Up to now, there is some preliminary research on FAV vector in foreign countries, while there was little research progress in China. The CMV promoter is used more popularly than the major late promoter of FAV in the construct of recombinant virus, but which one is more efficient to express the foreign gene is unknown.
In this study, fowl adenoviruses (FAV) distributions in flocks of chickens, ducks and geese were investigated by PCR. The results show that FAV was extensively popular in chickens, geese and ducks, with the positive rate of 51.4%, 51.7%, and 17.3%, respectively. Three positive samples were selected separately from chickens, ducks and geese, and then the open reading frame 8 (ORF8) and the right terminal of viral genome (ITR), were amplified, sequenced and analyzed. All nine isolates were 94.7%-99.9% homologous to FAVI when compared with that of CELOV, FAVI-JS, but shared low nucleotide sequence homology to FAV-9 and EDSV. They all converged into the same lineage with CELOV, FAVI-JS, while the viruses from group III shared the other one. The sequence of the ORF8 from all the above fowl adenoviruses shared very high homology to each other, which is in accordance with the genomic structural feature of adenovirus. From these results, we can presume that the fowl adenoviruses isolates belong to FAV group I.
In order to investigate the possibility of FAV as live vaccine vector, the biological characterization of FAVs isolates were further studied with technology of electron microscope, virus morphology, cytopathogenic effect (CPE) and experiments in ovo and in vivo. The results of these experiments showed that the virus particles are spherical, non-enveloped, and about 70-80nm in diameter and couldn't agglutinate the erythrocytes of chickens. The virus couldn't cause CPE in chicken embryo fibroblast cells (CEFs), but cause typical CPE of FAV in chicken embryonic kidney cells (CEKs), which became round with stronger light refraction. No gross lesions and growth retardation was observed in chicken embryo inoculated with the isolates, which are not completely in conformity with the characterization of CELOV (Chicken embryo lethal orphan virus).
To evaluate the safety of isolated viruses, SPF chickens were inoculated with the FAVI-G24-0605 at 1-day-old by subcutaneous injection or oral way. The weight of body and some immune organs were measured when a couple of chicken was euthanized at 10d, 20d, and 30d post infection (p.i). The results showed that the inoculated chickens were all healthy with no gross lesions during the animal experiment and there were no serious effects on the body weight of SPF chickens, although the body weight of chickens was lower than the control at 10d p.i and became normal at 20d and 30d p.i. The same changes also occurred in the weight of major immune organs of chickens inoculated. Most of tissue samples had no histopathological changes except some minor lesions at the early stage. These results indicate that the FAVI-G24-0605 strain may be a good candidate for recombinant FAV.
The non-essential region for replication of FAVI-JS was determined and the plasmid pFACMV3.1, a universal transfer vector for gene transferred to FAVI-JS, has been constructed by our studying team. On the basis of this research, FAVI-JS was selected from all the fowl adenovirus isolated in our lab for recombination. The major late promoter (MLP) of FAVI-JS, which belongs to group I avian adenovirus, were amplified by PCR and the sequence of MLP has high homology (99%) with that of CELOV. Then the MLP, together with a fragment from pFACMV3.1, containing the R fragment and the large part of L fragment, were cloned into pcDNA3.1/zeo (+). The above large fragment, digested with another two restrict enzymes, was subcloned to pFACMV3.1 to produce pFAMLP3.1, an universal transfer vector controlled by the MLP. By the same way, the ORF of eGFP gene was cloned into pFAMLP3.1 to construct pFAMLP-eGFP as a transfer vector with eGFP controlled by the MLP.
CEK was transfected with pFAMLP-eGFP plasmid. Expression of eGFP was found at 48h post transfection. Then CEK infected with wt-FAVI-JS was transfected with pFAMLP-eGFP to produce recombinant virus. The transfected cells and supernatant was freezed, thawed and centrifuged after 3-4d. And the diluted supernatant was re-infected CEK. The expression of eGFP was observed for at least 3 passages. Thus the recombinant fowl adenovirus rFAMLP-eGFP was successfully constructed and provided the basis for study the influence of the promoter on the immune efficiency of FAV.
引文
[1]Rigky PWJ.Cloning vectors derived from animal viruses.J.Gen.Virol.,1983,64:255.
[2]侯云德.分子病毒学.北京:学苑出版社.1990.151
[3]Szilvia L,Farkas M,Benk P,et al.Genomic and phylogenetic analyses of an adenovirus isolated from a corn snake(Elaphe guttata) imply a common origin with members of the proposed new genus Atadenovirus[J].Journal of General Virology,2002,83:2403-2410.
[4]Duncan J,Mc Geoch,Derek Gatherer.Integrating reptilian herpesviruses into the family herpesviridae[J].Journal of Virology,2005,79(2):725-731.
[5]Fauquet C M,Mayo M A,Maniloff J,et al.Virus Taxonomies 8th Report of ICTV[M].Amsterdaml:Elsevier Academic Press,2005.
[6]张维维.腺病毒载体及其在基因治疗中的应用.肿瘤分子生物学研究进展.96北京国际肿瘤分子生物学学术会议论文汇编.1996:30-41
[7]殷震 刘景华 主编.动物病毒学(第二版).科学出版社.1997.1104-1129.
[8]Corredor JC,Krell PJ,Nagy E,Sequence analysis of the left end of fowl adenovirus genomes.Virus Genes.2006 Aug;33(1):95-106.
[9]Corredor JC,Garceac A,Krell PJ,et al.Sequence comparison of the right end of fowl adenovirus genomes.Virus Genes.2008.36(2):331-44.
[10]Poi Kiang Tan,Anne-Isabelle Michou,Jeffrey M,et al.Defining CAR as a cellular receptor for the avian adenovirus CELO using a genetic analysis of the two viral fibre proteins,J of Gen Virol,(2001),82,1465-1472.
[11]Maria C B,Karen S,Yian-Biao Z,Paul F,John M F.Structural analysis of the mechanism of adenovirus binding to its human celluar receptor,CAR.Science,1999,286(19):1579-1583.
[12]Taharaguchi S,Kono Y,Ohta H,et al.Putative host cell receptor for fowl adenovirus detected in gizzard.J Vet Med Sci.2007.69(11):1203-5.
[13]Kiseleva EK,Khil'Ko SN,Grigor'ev VG,et al.Stability of the structure and antigenic determinants of adenovirus type 1 native hexon to proteases.Biokhimiia,1986,51(8):1286-294.
[14]Zask L,Kisary J.Grouping of fowl adenoviruses based upon the restriction pattems of DNA generated by BamH Ⅰ and Hind Ⅲ.Intervirology,1984,22(2):110-114
[15]Zask L,Kisary J.Studies on egg drop syndrome(EDS) and chick embryo lethal orphan (CELO) avian adenovirus DNAs by restriction endonucleases.J Gen Virol,1981,56(Pt1):87-95
[16]Shimada K,Yoshida T,Kutoishi T,et al.Restriction map of CELO virus DNA.Biochemica Biophysica Acta,1983,740:169-178
[17]Shinagawa M,Iida Y,Matsuda A,et al.Phylogenetic relationships between adenovirus as inferred from nucleotide sequences of inverted terminal repeats.Gene,1987,55:85-93
[18]Sheppard M,Erny K M.DNA sequence analysis of the inverted terminal repeats of a non-oncogenic avian adenovirus.Nucleic Acids Research,1989,17(10):3995
[19]Akopian T A,Kruglyak V A,Rivkina M B,et al.Sequence of an avian adenovirus(CELO)DNA fragment(0-11.2%).Nucleic Acids Research,1990,18(9):2825
[20]Rademaker HJ,Fallaux FJ,Van den Wollenberg DJ,et al.Relaxed template specificity in fowl adenovirus 1 DNA replication initiation.J Gen Virol.2006 Mar;87(Pt 3):553-62.
[21]Li P,Bellett A J D,Parish C R.The structural proteins of chick embryo lethal orphan virus (fowl adenovirus type 1).Journal of General Virology,1984,65(10):1803-1815
[22]Tan P K,Miehou A I,Bergelson J M,et al.Defining CAR as a celluar receptor for the avian adenovirus CELO using a genetic analysis of the two viral fibre proteins.J Gen Virol,2001,82(Pt6):1465-72
[23]王凤雪 王云峰 智海东 等.CELO病毒fiber1基因部分片段的原核表达及抗原性鉴定.中国兽医科技.2005.35(6):468-471
[24]Parthiban M,Manoharan S,Roy P,et al.Nucleotide sequence analysis of the L1 loop variable region of hexon gene of fowl adenovirus 4 isolates from India.Acta Virol.2005;49(1):65-8
[25]Chiocca S,Kurtev V,Colombo R,et al.Histone deacetylase 1 inactivation by an adenovirus.early gene product.Curr Biol,2002,12(7):594-8
[26]Glotzer J B,Saltik M,Chiocca S,et al.Activation of heat-shock response by an adenovirus is essential for virus replication.Nature,2000,407(6801):207-11
[27]Misiurina O,Neugodova G L,Naroditskii B S.Transcription of anti-apoptotic proteins from CELO and EDS avian adenovirus at early stages of infection.Mol Gen Mikrobiol Virusol,2000,(4):15-7
[28]Chiocca S,Baker A,Cotton M.Identification of a novel antippotitic protein,GAM-1,encoded by the CELO adenovirus.J Virol,1997,71(4):3168-77
[29]Hacker D L,Derow E,Wurm F M.The CELO adenovirus Gaml protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells.J Biotechnol,2005,117(1):21-9
[30]Wick N,Luedemann S,Vietor I,et al.Induction of short interspersed nuclear repeat-containing transcripts in epithelial cells upon infection with a chicken adenovirus.J Mol Biol.2003 May 9;328(4):779-90.
[31]Hess M,Blocker H,Brant P.The complete nucleotide sequence of egg drop syndrome virus:an intermediate between mastadenoviruses and aviadenoviruses.Virology,1997,238(1):145-56
[32]金奇 曾力宇.禽减蛋综合征病毒AAV2株全基因组文库的构建及核苷酸序列分析.中国科学:C辑.1999.29(5):543-548
[33]李刚 周锦屏 郑明球 等.鸭源和鸡源减蛋综合征病毒(EDSV)酶切图谱的分析.中国兽医学报.1997.17(6):551-554
[34]金奇 曾力宇 李茂祥 等.鸡减蛋综合征病毒(EDSV-76)基因组E1区结构特点分析.病毒学报.1998.14(3):253-255
[35]曾力宇 单金刚.鸡减蛋综合征病毒(EDSV)的基因组特点.科学通报.1998.43(5):524-527
[36]李虹 金奇 章金钢 等.鸡减蛋综合征病毒(EDSV)基因组右末端结构的分析,病毒学报,1998,14(4):329-337
[37]杜华茂 黄伟 谢和芬 等.含PHYA基因的EDSV转移载体构建和表达研究.西南农业大学学报.2005.27(1):132-135
[38]Yamaguchi S,Imada T,Kawamura H,et al.Pathogenicity and distribution of egg-drop syndrome-1976virus(JPA-1) in inoculated laying hens.Avian Dis,1981,25(3):642-9
[39]俞乃胜 龙沛然 等.鹌鹑腺病毒的分离和病原特性研究.畜牧兽医学报.2002.33(3):271-275
[40]张春杰 张敏 吴庭才 等.EDSV六邻体蛋白基因克隆与原核表达载体构建,河南科技大学学报(自然科学版),2005,26(4):80-83
[41]Chiocca S,Kuzbauer R,Schaffner G,et al.The complete DNA sequence and geomic organization of the avian adenovirus CELO.J Virol,1996,70:2939-2949
[42]潭维彦 阮力 彭薇等.腺病毒4型E3区核苷酸序列测定及其基因结构与功能分析.病毒学报.1994.10:197-208
[43]Reddy P S,Idamakanti N,Derbyshire J B,et al.Porcine adenovirus types 1,2 and 3 have short and simple early E3 regions.Virus Research,1996,43:99-109
[44]Zakharchuk A N,Kruglyak V A,A kopian T A,et al.Physical mapping and homology studies of egg drop syndrome(EDS276) adenovirus DNA.Arch Virol,1993,128:171-176
[45]王雪敏 蔡宝祥 郑明球 等.单抗夹心ELISA检测产蛋下降综合征病毒的研究.中国兽医杂志.1998.24(3):8-9
[46]Raj GD,Ratnapraba S,Matheswaran K,et al.Comparison of hemagglutination inhibition test and ELISA in quantification of antibodies to egg drop syndrome virus.Acta Vitrol.2004;48(3):183-7.
[47]王爱华 王玉珠 孙继国 等.胶体金快速诊断方法对鸡减蛋综合征病毒的检测.中国兽医科技.2005.35(11):856-858
[48]曹红敏 徐学孟 王文志 等.光敏生物素标记EDSV-DNA探针检测减蛋综合征病毒的研究.动物检疫.1994.11(1):7-8
[49]张春杰 李银聚 程相朝 等.应用降落PCR检测减蛋综合征病毒的研究.中国兽医科技.2003.33(7):11-13
[50]Kumar NS,Kataria JM,Koti M,et al.Detection of egg drop syndrome 1976 virus by polymerase chain reaction and study of its persistence in experimentally infected layer birds.Aeta Virol.2003;47(3):179-84
[51]Fingerut E,Gutter B,Gallili G,et al.A subunit vaccine against the adenovirus egg-drop syndrome using part of its fiber protein.Vaccine.2003 Jun 20;21(21-22):2761-6.
[52]Adrych K.Can obesity be infectious? Przegl Lek.2005;62(9):916-8.
[53]Jaworowska A,Bazylak G.Obesity development associated with viral infections.Postepy Hig Med Dosw(Online).2006;60:227-36.
[54]富强 翟新验 卢胜明.3Rs原则的应用实例-用鸡胚肝细胞替代鸡肾细胞培养禽腺病毒.实验动物科学与管理.2003.20(3):54-56
[55]B.W.Carlnek,Disease of poultry,第十版,中国农业出版社,782-783
[56]Schonewille E,Singh A,Gobel TW,et al.Fowl adenovirus(FAdV) serotype 4 causes depletion of B and T cells in lymphoid organs in specific pathogen-free chickens following experimental infection.Vet Immunol Immunopathol.2008.121(1-2):130-9.
[57]Zavala G,Dufour-Zavala L,Villegas P,et al.Lack of interaction between avian leukosis virus subgroup J and fowl adenovirus(FAV) in FAV-antibody-positive chickens.Avian Dis.2002.Oct-Dec;46(4):979-84.
[58]Muroga N,Taharaguchi S,Ohta H,et al.Pathogenicity of fowl adenovirus isolated from gizzard erosions to immuno-suppressed chickens.J Vet Med Sci.2006 Mar;68(3):289-91.
[59]Rautenschlein S,Sharma JM.Response of turkeys to simultaneous vaccination with hemorrhagic enteritis and Newcastle disease viruses.Avian Dis.1999 Apr-Jun;43(2):286-92
[60]Chary P,Rautenschlein S,Sharma JM.Reduced efficacy of hemorrhagic enteritis virus vaccine in turkeys exposed to avian pneumovirus.Avian Dis.2002 Apr-Jun;46(2):353-9.
[61]Balamurugan V,Kataria JM.Economically important non-oncogenic immunosuppressive viral diseases of chicken—current status.Vet Res Commun.2006 Jul;30(5):541-66.
[62]Anne-Isabelle Michouy,Heike Lehrmann and Mediyha Saltik,et al.Mutational Analysis of the Avian Adenovirus CELO,Which Provides a Basis for Delivery Vectors.J.Virol.,1999,73(2):1399-1410.
[63]Achille Francois,Nicolas Eterradossi,Bernard Delmas,et al.Construction of Avian Adenovirus CELO Recombinants in Cosmids.J.Virol.,2001,75(11):5288-5301.
[64]Washietl S,Eisenhaber F.Reannotation of CELO genome characterizes a set of previously unassigned open reading frames and points to vivo modes of host interaction in avian adenoviruses.BMC Bioinfermatics,2003,4(1):55.
[65]Le Goff F,Mederle-Mangeot Ⅰ,Jestin A,et al.Deletion of open reading flames 9,10 and 11from the avian adenovirus CELO genome:effect on biodistribution and humoral responses.J Gen Virol.2005 Jul;86(Pt 7):2019-27
[66]Achille Francois,Christophe Chevalier,Bernard Delmas,et al.Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens.Vaccine.2004 Jun 2;22(17-18):2351-60
[67]Sheppard M,W Wemer and E Tsatas,et al.Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease.Achives of Virology,1998,143:915-930.
[68]Davor Ojkic,Nagy E.The long repeat region is dispensable for fowl adenovirus replication in vitro.Virology,2001,283(2):197-206.
[69]Ojkic D,Nagy E.The complete nucleotide sequence of fowl adenovirus type 8.J Gen Virol.2000,81(7):1833-1837
[70]Davor Ojkic and Eva Nagy.Antibody response and virus tissue distribution in chickens inoculated with wild-type and recombinant fowl adenoviruses.Vaccine,2003,22(1):42-48.
[71]Cao,J.X.,Krell,P.J.,and Nagy.Sequence and transcriptional analysis of terminal regions of the fowl adenovirus type 8 genome.J.Gen.Virol.,1998,79:2507-2516.
[72]Michael A.Johnson,Cathryn Pooley,John W.Lowenthal.Delivery of avian cytokines by adenovirus vectors.Developmental and Comparative Immunology,2000,24:343-354.
[73]Michael A.Johnson,Catherine Pooley,Jagoda Ignjatovic,et al.A recombinant fowl adenovirus expressing the S 1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis vires.Vaccine,2003,21:2730-2736.
[74]Gao W,Soloff AC,Lu X,et al.Protection of mice and poultry from lethal H5N1 avian influenza virus through adenovirus-based immunization.J Virol.2006 Feb;80(4):1959-64.
[75]何秀苗 秦爱建 刘岳龙等.禽腺病毒江苏株(JS)复制非必需片段的确定.微生物学报.2004.44(3):23-27
[76]刘岳龙 徐福州 崔治中 等.鹅源腺病毒Y81G4株基因组末端倒置重复序列(ITR)的鉴定和结构分析.中国预防兽医学报.2000.22(4):285-288.
[77]郑玉美 王永坤 徐瑗 等.鹅源腺病毒的初步研究.中国家禽.1992.(6):23-24.
[78]徐福州 崔治中 刘岳龙 等.鹅源腺病毒基因组DNA物理图谱的构建.中国病毒学.2000.15(4):288-294.
[79]刘岳龙 秦爱建 金文杰 等.鹅源腺病毒基因组E3区的鉴定和分析.江苏青年微生物学学术报告会论文集.江苏省微生物学会.江苏南京.2001.12.281-185.
[80]王凤雪 王云峰.CELOV载体在人类和动物疾病控制上的应用.中国生物工程杂志.2005.25(1):14-17
[81]陈溥言.禽腺病毒Ⅰ型(鸡胚致死孤儿病毒)在非免疫鸡胚中隐性感染可能已经严重危害到我国疫苗生产.畜牧与兽医.2002.34(6):19
[82]周斌 刘华雷 曹瑞兵 等.污染疫苗的禽腺病毒分离和鉴定.南京农业大学学报.2004.27(3):78-80
[83]Logunov Dlu,Cherenova LV,Shmarov MM,et al.Delivery of secreted placental 1 alkaline phosphatase(SEAP) gene in vitro and in vivo as a component of recombinant avian adenovirus (CELO).Mol Gen Microbiol Virusol.2002,4:21-25.
[84]Cherenova LV,Logunov DY,Shashkova EV,et al.Recombinant avian adenovirus CELO expressing the human interleukin-2:characterization in vitro,in ovo and in vivo.Virus Res.2004Mar 15;100(2):257-61.
[85]Logunov DY,Ilyinskaya GV,Cherenova LV,et al.Restoration of P53 tumor-suppressor acting in human tumor cells in vitro and in their exnografts in vivo by recombinant avian adenovirus CELO-p53.Gene Ther.2004,11(1):79-84.
[86]Shashkova EV,Cherenova LV,Kazansky DB,et al.Avian adenovirus vector CELO-TK displays anticancer activity in human cancer cells and suppresses established murine melanoma tumors.Cancer Gene Ther.2005 Jul;12(7):617-26.
[87]Avdeeva SV,Voronov DA,Khaidarova NV,et al.The CELO-ANG recombinant avian adenovirus with human angiogenine gene inducing neovascularization in the anterior tibial muscle of rat,Mol Gen Mikrobiol Virusol.2004;(4):38-40
[88]Ding H,Wang R,Marcel R,Fisher DZ,et al.Adenovirus-mediated expression of a truncated PDGF beta receptor inhibits thrombosis and neointima formation in an avian arterial injury model.Thromb Haemost.2001 Sep;86(3):914-22
[89]Stevenson M,Boos E,Herbert C,et al.Chick embryo lethal orphan virus can be polymer-coated and retargeted to infect mammalian cells.Gene Ther.2006 Feb;13(4):356-68.
[1]陈溥言.禽腺病毒1型(鸡胚致死孤儿病毒)在非免疫鸡胚中隐性感染可能己经严重危害到我国疫苗生产.畜牧与兽医.2002,34(6):19.
[2]周斌 曹瑞兵 卢银华等.随机引物PCR扩增鸡胚致死孤儿病毒DNA的研究.中国病毒学.2003,18(2):137-140.
[3]Sam blook等著.金冬雁等译.分子克隆(实验指南).科学出版社.1996.第二版
[4]Dhinakar Raj G,Sivakumar S,Matheswaran K,et al.Detection of egg drop syndrome virus antigen or genome by enzyme-linked immunosorbent assay or polymerase chain reaction.Avian Pathol.2003 Oct;32(5):545-50.
[5]Kumar NS,Katafia JM,Koti M,et al.Detection of egg drop syndrome 1976 virus by polymerase chain reaction and study of its persistence in experimentally infected layer birds.Acta Virol.2003;47(3):179-84.
[6]Raj GD,Ratnapraba S,Matheswaran K,et al.Comparison of hemagglutination inhibition test and ELISA in quantification of antibodies to egg drop syndrome virus.Acta V'trol.2004;48(3):183-7.
[7]Schrenzel M,Oaks JL,Rotstein D,et al.Characterization of a new species of adenovirus in falcons.J Clin Microbiol.2005 Jul;43(7):3402-13.
[8]Uhart MM,Quintana F,Karesh WB,et al.Hematology,plasma biochemistry,and serosurvey for selected infectious agents in southern giant petrels from Patagonia,Argentina.J Wildl Dis.2003 Apr;39(2):359-65.
[9]Raue R,Gerlach H,Muller H.Phylogenetic analysis of the hexon loop 1 region of an adenovirus from psittacine birds supports the existence of a new psittaeine adenovirus(PsAdV).Arch Virol.2005Oct;150(10):1933-43.
[10]Hollmen TE,Franson JC,Flint PL,et al.An adenovirus linked to mortality and disease in long-tailed ducks(Clangula hyemalis) in Alaska.Avian Dis.2003 Oct-Dec;47(4):1434-40.
[11]Guy JS,Barnes HJ,Smith L,et al.Partial characterization of an adenovirus-like virus isolated from broiler chickens with transmissible viral proventrieulitis.Avian Dis.2005 Sep;49(3):344-51.
[12]Morikazu Shinagawa,Yoichi Iida,Akihisa,et al.Phylogenetic relationships between adenoviruses as inferred from nucleotide sequences of inverted terminal repeats.Gene,1987,55:85-93.
[13]Michael Hess,Helmut Blocker,Petra Brandt.The Complete Nucleotide Sequence of The Egg Drop Syndrome Virus:An Intermediate between Mastadenoviruses and Aviadenoviruses.Virology,1997,238:145-156.
[14]刘岳龙,徐福州,崔治中等.鹅源腺病毒Y81G4株基因组末端倒置重复序列(ITR)的鉴定和结构分析.中国预防兽医学报.2000,22(4):285-288.
[15]Chiocca S,Kurtev V,Colombo R,et al.Histone deacetylase 1 inactivation by an adenovirus early gene product.Curr Biol,2002,12(7):594-8
[16]Misiurina O,Neugodova G L,Naroditskii B S.Transcription of anti-apoptotic proteins from CELO and EDS avian adenovirus at early stages of infection.Mol Gen Mikrobiol Virusol,2000,(4):15-7
[17]Chiocca S,Baker A,Cotton M.Identification of a novel antippotitic protein,GAM-1,encoded by the CELO adenovirus.J Virol,1997,71(4):3168-77
[18]Hacker D L,Derow E,Wurm F M.The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells.J Biotechnol,2005,117(1):21-9
[1]Szilvia L,Farkas M,Benk P,et al.Genomic and phylogenetic analyses of an adenovirus isolated from a corn snake(Elaphe guttata) imply a common origin with members of the proposed new genus Atadenovirus [J].Journal of General Virology,2002,83:2403-2410.
[2]Duncan J,Mc Geoch,Derek Gat herer.Integrating reptilian herpesviruses into the family herpesviridae[J].Journal of Virology,2005,79(2):725-731.
[3]Fauquet C M,Mayo M A,Maniloff J,et al.Virus Taxonomies 8th Report of ICTV[M].Amsterdaml:Elsevier Academic Press,2005.
[4]富强 翟新验 卢胜明.3Rs原则的应用实例-用鸡胚肝细胞替代鸡肾细胞培养禽腺病毒.实验动物科学与管理.2003.20(3):54-56
[5]殷震 刘景华 主编.动物病毒学(第二版).科学出版社.1997.
[6]B.W.Calnet et al.Disease of Poultry[M].Tenth Edition.1997;607-642
[7]B.W Carlnek,et al.Disease of Poultry,第十版,中国农业出版社,782-783
[8]Schonewille E,Singh A,Gobel TW,et al.Fowl adenovirus(FAdV) serotype 4 causes depletion of B and T cells in lymphoid organs in specific pathogen-free chickens following experimental infection.Vet Immunol Immunopathol.2008.121(1-2):130-9.
[9]甘孟侯.中国禽病学.中国农业出版社.1999,94-104
[10]Cook J.K.A.Pathogenicity of avian adenovirus for day-old chicks.J Comp Pathol,1974,84:505-515
[11]Barr D.A,P.Scott.Adenovirus and IBH.Proc 2~(nd) Asian/Pacific poult health conf[Proc 112].Post Gruduate Comm Vet Sci,University of Sydney,Austrilia,323-326
[12]Yates V J,Rhee Y O,Fry D E.Serological response of chickens exposed to a type 1 avian adenovirus alone or in combination with the adeno-associated virus.Avian Dis.1977,21:408-14.
[1]Chiocca S,Kurzbauer R,Schaffner G,et al.The complete DNA sequence and genomic organization of the avian adenovirus CELO.J Virol.1996 May;70(5):2939-49.
[2]Ojkic D,Nagy E.The complete nucleotide sequence of fowl adenovirus type 8.J Gen Virol.2000 Jul;81(Pt 7):1833-7.
[3]Hess M,Blocker H,Brandt P.The complete nucleotide sequence of the egg drop syndrome virus:an intermediate between mastadenoviruses and aviadenoviruses.Virology.1997 Nov 10;238(1):145-56.
[4]Pitcovski J,Mualem M,Rei-Koren Z,et al.The complete DNA sequence and genome organization of the avian adenovirus,hemorrhagic enteritis virus.Virology.1998 Sep 30;249(2):307-15.
[5]Achille Francois,Nicolas Eterradossi,Bernard Delmas,et al.Construction of Avian Adenovirus CELO Recombinants in Cosmids.J.Virol.,2001,75(11):5288-5301.
[6]Achille Francois,Christophe Chevalier,Bernard Delmas,et al.Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens.Vaccine.2004 Jun 2;22(17-18):2351-60
[7]Sheppard M,W Werner and E Tsatas,et al.Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces pretective immunity against bursal disease.Achives of Virology,1998,143:915-930.
[8]Michael A.Johnson,Cathryn Pooley,John W.Lowenthal.Delivery of avian cytokines by adenovirus vectors.Developmental and Comparative Immunology,2000,24:343-354.
[9]Michael A.Johnson,Catherine Pooley,Jagoda Ignjatovic,et al.A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus.Vaccine,2003,21:2730-2736.
[10]Davor Ojkic,Nagy E.The long repeat region is dispensable for fowl adenovirus replication in vitro.Virology,2001,283(2):197-206.
[11]Davor Ojkic and Eva Nagy.Antibody response and virus tissue distribution in chickens inoculated with wild-type and recombinant fowl adenoviruses.Vaccine,2003,22(1):42-48.
[12]何秀苗 张科 秦爱建等.Ⅰ群禽腺病毒江苏分离株(FA-V-JS)的分离鉴定.中国预防兽医学报. 2005.27(1):42-45
[13]何秀苗 秦爱建 刘岳龙等.禽腺病毒江苏株(JS)复制非必需片段的确定.微生物学报.2004.44(3):23-27
[14]Sam blook等著.金冬雁等译.分子克隆(实验指南).科学出版社.1996.第二版
[15]Addison C L,Hitt M,Kunsken D,et al.Comparison of the human versus murine cytomegalovirus immediate early gene promoters for transgene expression by adenovirus vectors.J Virol.,1997,78(7):1653-1661.
[16]Payet V,Arnauld C,Picault JP,et al.Transcriptional organization of the avian adenovirus CELO.J Virol.1998 Nov;72(11):9278-85.
[17]Sheppard M,Werner W,McCoy RJ,et al.The major late promoter and bipartite leader sequence of fowl adenovirus.Arch Virol.1998;143(3):537-48.
[18]Leong K,Lee W,Berk AJ(1990) High-level transcription from the adenovirus major late promoter requires downstream binding sites for late-phase-specific factors.J Virol 64:51-60
[19]Li X-C,Huang WL,Flint SJ(1992) The downstream regulatory sequence of the adenovirus type 2 major late promoter is fuctionally redundant.J Virol 66:5 685-5 690
[20]Mondesert G,Kedinger C(1991) Cooperation between upstream and downstream elements of the adenovirus major late promoter for maximal late phase-specific transcription.Nucleic Acids Res 19:3 221-3228
[1]Yates V J,Rhee Y O,Fry D E.Serological response of chickens exposed to a type 1 avian adenovirus alone or in combination with the adeno-associated virus.Avian Dis.,1977,21:408-414.
[2]Cherenova LV,Logunov DY,Shashkova EV,et al.Recombinant avian adenovirus CELO expressing the human interleukin-2:characterization in vitro,in ovo and in vivo.Virus Res.2004 Mar 15;100(2):257-61.[3]Francois A,Eterradossi N,Delmas B,et al.Construction of avian adenovirus CELO recombinants in cosmids.J Virol.2001 Jun;75(11):5288-301.
[4]Francois A,Chevalier C,Delmas B,et al.Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens.Vaccine.2004 Jun 2;22(17-18):2351-60.
[5]Alexander HS,Huber P,Cao J,et al.Growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line.J Virol Methods.1998 Sep;74(1):9-14.
[6]Scholz E,Welniak E,Nyholm T,et al.An avian hepatoma cell line for the cultivation of infectious laryngotracheitis virus and for the expression of foreign genes with a mammalian promoter.J Virol Methods.1993 Aug;43(3):273-86.
[7]Payet V,Arnauld C,Picault JP,et al.Transcriptional organization of the avian adenovirus CELO.J Virol.1998 Nov;72(11):9278-85.
[8]Sheppard M,Werner W,McCoy RJ,et al.The major late promoter and bipartite leader sequence of fowl adenovirus.Arch Virol.1998;143(3):537-48.
[9]Tsukamoto K,Saito S,Saeki S,et al.Complete long lasting protection against lethal infectious bursal disease virus challenge by a single vaccination with an avian herpesvirus vector expressing VP2 antigens[J].J Virol,2002,76(11):5637-45.
[10]Sheppard M,W Wemer and E Tsatas,et al.Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease.Achives of Virology,1998,143:915-930.
[11]Michael A.Johnson,Cathryn Pooley,John W.Lowenthal.Delivery of avian cytokines by adenovirus vectors.Developmental and Comparative Immunology,2000,24:343-354.
[12]Michael A.Johnson,Catherine Pooley,Jagoda Ignjatovic,et al.A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus.Vaccine,2003,21:2730-2736.
[2]侯云德.分子病毒学.北京:学苑出版社.1990.151
[3]Szilvia L,Farkas M,Benk P,et al.Genomic and phylogenetic analyses of an adenovirus isolated from a corn snake(Elaphe guttata) imply a common origin with members of the proposed new genus Atadenovirus[J].Journal of General Virology,2002,83:2403-2410.
[4]Duncan J,Mc Geoch,Derek Gatherer.Integrating reptilian herpesviruses into the family herpesviridae[J].Journal of Virology,2005,79(2):725-731.
[5]Fauquet C M,Mayo M A,Maniloff J,et al.Virus Taxonomies 8th Report of ICTV[M].Amsterdaml:Elsevier Academic Press,2005.
[6]张维维.腺病毒载体及其在基因治疗中的应用.肿瘤分子生物学研究进展.96北京国际肿瘤分子生物学学术会议论文汇编.1996:30-41
[7]殷震 刘景华 主编.动物病毒学(第二版).科学出版社.1997.1104-1129.
[8]Corredor JC,Krell PJ,Nagy E,Sequence analysis of the left end of fowl adenovirus genomes.Virus Genes.2006 Aug;33(1):95-106.
[9]Corredor JC,Garceac A,Krell PJ,et al.Sequence comparison of the right end of fowl adenovirus genomes.Virus Genes.2008.36(2):331-44.
[10]Poi Kiang Tan,Anne-Isabelle Michou,Jeffrey M,et al.Defining CAR as a cellular receptor for the avian adenovirus CELO using a genetic analysis of the two viral fibre proteins,J of Gen Virol,(2001),82,1465-1472.
[11]Maria C B,Karen S,Yian-Biao Z,Paul F,John M F.Structural analysis of the mechanism of adenovirus binding to its human celluar receptor,CAR.Science,1999,286(19):1579-1583.
[12]Taharaguchi S,Kono Y,Ohta H,et al.Putative host cell receptor for fowl adenovirus detected in gizzard.J Vet Med Sci.2007.69(11):1203-5.
[13]Kiseleva EK,Khil'Ko SN,Grigor'ev VG,et al.Stability of the structure and antigenic determinants of adenovirus type 1 native hexon to proteases.Biokhimiia,1986,51(8):1286-294.
[14]Zask L,Kisary J.Grouping of fowl adenoviruses based upon the restriction pattems of DNA generated by BamH Ⅰ and Hind Ⅲ.Intervirology,1984,22(2):110-114
[15]Zask L,Kisary J.Studies on egg drop syndrome(EDS) and chick embryo lethal orphan (CELO) avian adenovirus DNAs by restriction endonucleases.J Gen Virol,1981,56(Pt1):87-95
[16]Shimada K,Yoshida T,Kutoishi T,et al.Restriction map of CELO virus DNA.Biochemica Biophysica Acta,1983,740:169-178
[17]Shinagawa M,Iida Y,Matsuda A,et al.Phylogenetic relationships between adenovirus as inferred from nucleotide sequences of inverted terminal repeats.Gene,1987,55:85-93
[18]Sheppard M,Erny K M.DNA sequence analysis of the inverted terminal repeats of a non-oncogenic avian adenovirus.Nucleic Acids Research,1989,17(10):3995
[19]Akopian T A,Kruglyak V A,Rivkina M B,et al.Sequence of an avian adenovirus(CELO)DNA fragment(0-11.2%).Nucleic Acids Research,1990,18(9):2825
[20]Rademaker HJ,Fallaux FJ,Van den Wollenberg DJ,et al.Relaxed template specificity in fowl adenovirus 1 DNA replication initiation.J Gen Virol.2006 Mar;87(Pt 3):553-62.
[21]Li P,Bellett A J D,Parish C R.The structural proteins of chick embryo lethal orphan virus (fowl adenovirus type 1).Journal of General Virology,1984,65(10):1803-1815
[22]Tan P K,Miehou A I,Bergelson J M,et al.Defining CAR as a celluar receptor for the avian adenovirus CELO using a genetic analysis of the two viral fibre proteins.J Gen Virol,2001,82(Pt6):1465-72
[23]王凤雪 王云峰 智海东 等.CELO病毒fiber1基因部分片段的原核表达及抗原性鉴定.中国兽医科技.2005.35(6):468-471
[24]Parthiban M,Manoharan S,Roy P,et al.Nucleotide sequence analysis of the L1 loop variable region of hexon gene of fowl adenovirus 4 isolates from India.Acta Virol.2005;49(1):65-8
[25]Chiocca S,Kurtev V,Colombo R,et al.Histone deacetylase 1 inactivation by an adenovirus.early gene product.Curr Biol,2002,12(7):594-8
[26]Glotzer J B,Saltik M,Chiocca S,et al.Activation of heat-shock response by an adenovirus is essential for virus replication.Nature,2000,407(6801):207-11
[27]Misiurina O,Neugodova G L,Naroditskii B S.Transcription of anti-apoptotic proteins from CELO and EDS avian adenovirus at early stages of infection.Mol Gen Mikrobiol Virusol,2000,(4):15-7
[28]Chiocca S,Baker A,Cotton M.Identification of a novel antippotitic protein,GAM-1,encoded by the CELO adenovirus.J Virol,1997,71(4):3168-77
[29]Hacker D L,Derow E,Wurm F M.The CELO adenovirus Gaml protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells.J Biotechnol,2005,117(1):21-9
[30]Wick N,Luedemann S,Vietor I,et al.Induction of short interspersed nuclear repeat-containing transcripts in epithelial cells upon infection with a chicken adenovirus.J Mol Biol.2003 May 9;328(4):779-90.
[31]Hess M,Blocker H,Brant P.The complete nucleotide sequence of egg drop syndrome virus:an intermediate between mastadenoviruses and aviadenoviruses.Virology,1997,238(1):145-56
[32]金奇 曾力宇.禽减蛋综合征病毒AAV2株全基因组文库的构建及核苷酸序列分析.中国科学:C辑.1999.29(5):543-548
[33]李刚 周锦屏 郑明球 等.鸭源和鸡源减蛋综合征病毒(EDSV)酶切图谱的分析.中国兽医学报.1997.17(6):551-554
[34]金奇 曾力宇 李茂祥 等.鸡减蛋综合征病毒(EDSV-76)基因组E1区结构特点分析.病毒学报.1998.14(3):253-255
[35]曾力宇 单金刚.鸡减蛋综合征病毒(EDSV)的基因组特点.科学通报.1998.43(5):524-527
[36]李虹 金奇 章金钢 等.鸡减蛋综合征病毒(EDSV)基因组右末端结构的分析,病毒学报,1998,14(4):329-337
[37]杜华茂 黄伟 谢和芬 等.含PHYA基因的EDSV转移载体构建和表达研究.西南农业大学学报.2005.27(1):132-135
[38]Yamaguchi S,Imada T,Kawamura H,et al.Pathogenicity and distribution of egg-drop syndrome-1976virus(JPA-1) in inoculated laying hens.Avian Dis,1981,25(3):642-9
[39]俞乃胜 龙沛然 等.鹌鹑腺病毒的分离和病原特性研究.畜牧兽医学报.2002.33(3):271-275
[40]张春杰 张敏 吴庭才 等.EDSV六邻体蛋白基因克隆与原核表达载体构建,河南科技大学学报(自然科学版),2005,26(4):80-83
[41]Chiocca S,Kuzbauer R,Schaffner G,et al.The complete DNA sequence and geomic organization of the avian adenovirus CELO.J Virol,1996,70:2939-2949
[42]潭维彦 阮力 彭薇等.腺病毒4型E3区核苷酸序列测定及其基因结构与功能分析.病毒学报.1994.10:197-208
[43]Reddy P S,Idamakanti N,Derbyshire J B,et al.Porcine adenovirus types 1,2 and 3 have short and simple early E3 regions.Virus Research,1996,43:99-109
[44]Zakharchuk A N,Kruglyak V A,A kopian T A,et al.Physical mapping and homology studies of egg drop syndrome(EDS276) adenovirus DNA.Arch Virol,1993,128:171-176
[45]王雪敏 蔡宝祥 郑明球 等.单抗夹心ELISA检测产蛋下降综合征病毒的研究.中国兽医杂志.1998.24(3):8-9
[46]Raj GD,Ratnapraba S,Matheswaran K,et al.Comparison of hemagglutination inhibition test and ELISA in quantification of antibodies to egg drop syndrome virus.Acta Vitrol.2004;48(3):183-7.
[47]王爱华 王玉珠 孙继国 等.胶体金快速诊断方法对鸡减蛋综合征病毒的检测.中国兽医科技.2005.35(11):856-858
[48]曹红敏 徐学孟 王文志 等.光敏生物素标记EDSV-DNA探针检测减蛋综合征病毒的研究.动物检疫.1994.11(1):7-8
[49]张春杰 李银聚 程相朝 等.应用降落PCR检测减蛋综合征病毒的研究.中国兽医科技.2003.33(7):11-13
[50]Kumar NS,Kataria JM,Koti M,et al.Detection of egg drop syndrome 1976 virus by polymerase chain reaction and study of its persistence in experimentally infected layer birds.Aeta Virol.2003;47(3):179-84
[51]Fingerut E,Gutter B,Gallili G,et al.A subunit vaccine against the adenovirus egg-drop syndrome using part of its fiber protein.Vaccine.2003 Jun 20;21(21-22):2761-6.
[52]Adrych K.Can obesity be infectious? Przegl Lek.2005;62(9):916-8.
[53]Jaworowska A,Bazylak G.Obesity development associated with viral infections.Postepy Hig Med Dosw(Online).2006;60:227-36.
[54]富强 翟新验 卢胜明.3Rs原则的应用实例-用鸡胚肝细胞替代鸡肾细胞培养禽腺病毒.实验动物科学与管理.2003.20(3):54-56
[55]B.W.Carlnek,Disease of poultry,第十版,中国农业出版社,782-783
[56]Schonewille E,Singh A,Gobel TW,et al.Fowl adenovirus(FAdV) serotype 4 causes depletion of B and T cells in lymphoid organs in specific pathogen-free chickens following experimental infection.Vet Immunol Immunopathol.2008.121(1-2):130-9.
[57]Zavala G,Dufour-Zavala L,Villegas P,et al.Lack of interaction between avian leukosis virus subgroup J and fowl adenovirus(FAV) in FAV-antibody-positive chickens.Avian Dis.2002.Oct-Dec;46(4):979-84.
[58]Muroga N,Taharaguchi S,Ohta H,et al.Pathogenicity of fowl adenovirus isolated from gizzard erosions to immuno-suppressed chickens.J Vet Med Sci.2006 Mar;68(3):289-91.
[59]Rautenschlein S,Sharma JM.Response of turkeys to simultaneous vaccination with hemorrhagic enteritis and Newcastle disease viruses.Avian Dis.1999 Apr-Jun;43(2):286-92
[60]Chary P,Rautenschlein S,Sharma JM.Reduced efficacy of hemorrhagic enteritis virus vaccine in turkeys exposed to avian pneumovirus.Avian Dis.2002 Apr-Jun;46(2):353-9.
[61]Balamurugan V,Kataria JM.Economically important non-oncogenic immunosuppressive viral diseases of chicken—current status.Vet Res Commun.2006 Jul;30(5):541-66.
[62]Anne-Isabelle Michouy,Heike Lehrmann and Mediyha Saltik,et al.Mutational Analysis of the Avian Adenovirus CELO,Which Provides a Basis for Delivery Vectors.J.Virol.,1999,73(2):1399-1410.
[63]Achille Francois,Nicolas Eterradossi,Bernard Delmas,et al.Construction of Avian Adenovirus CELO Recombinants in Cosmids.J.Virol.,2001,75(11):5288-5301.
[64]Washietl S,Eisenhaber F.Reannotation of CELO genome characterizes a set of previously unassigned open reading frames and points to vivo modes of host interaction in avian adenoviruses.BMC Bioinfermatics,2003,4(1):55.
[65]Le Goff F,Mederle-Mangeot Ⅰ,Jestin A,et al.Deletion of open reading flames 9,10 and 11from the avian adenovirus CELO genome:effect on biodistribution and humoral responses.J Gen Virol.2005 Jul;86(Pt 7):2019-27
[66]Achille Francois,Christophe Chevalier,Bernard Delmas,et al.Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens.Vaccine.2004 Jun 2;22(17-18):2351-60
[67]Sheppard M,W Wemer and E Tsatas,et al.Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease.Achives of Virology,1998,143:915-930.
[68]Davor Ojkic,Nagy E.The long repeat region is dispensable for fowl adenovirus replication in vitro.Virology,2001,283(2):197-206.
[69]Ojkic D,Nagy E.The complete nucleotide sequence of fowl adenovirus type 8.J Gen Virol.2000,81(7):1833-1837
[70]Davor Ojkic and Eva Nagy.Antibody response and virus tissue distribution in chickens inoculated with wild-type and recombinant fowl adenoviruses.Vaccine,2003,22(1):42-48.
[71]Cao,J.X.,Krell,P.J.,and Nagy.Sequence and transcriptional analysis of terminal regions of the fowl adenovirus type 8 genome.J.Gen.Virol.,1998,79:2507-2516.
[72]Michael A.Johnson,Cathryn Pooley,John W.Lowenthal.Delivery of avian cytokines by adenovirus vectors.Developmental and Comparative Immunology,2000,24:343-354.
[73]Michael A.Johnson,Catherine Pooley,Jagoda Ignjatovic,et al.A recombinant fowl adenovirus expressing the S 1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis vires.Vaccine,2003,21:2730-2736.
[74]Gao W,Soloff AC,Lu X,et al.Protection of mice and poultry from lethal H5N1 avian influenza virus through adenovirus-based immunization.J Virol.2006 Feb;80(4):1959-64.
[75]何秀苗 秦爱建 刘岳龙等.禽腺病毒江苏株(JS)复制非必需片段的确定.微生物学报.2004.44(3):23-27
[76]刘岳龙 徐福州 崔治中 等.鹅源腺病毒Y81G4株基因组末端倒置重复序列(ITR)的鉴定和结构分析.中国预防兽医学报.2000.22(4):285-288.
[77]郑玉美 王永坤 徐瑗 等.鹅源腺病毒的初步研究.中国家禽.1992.(6):23-24.
[78]徐福州 崔治中 刘岳龙 等.鹅源腺病毒基因组DNA物理图谱的构建.中国病毒学.2000.15(4):288-294.
[79]刘岳龙 秦爱建 金文杰 等.鹅源腺病毒基因组E3区的鉴定和分析.江苏青年微生物学学术报告会论文集.江苏省微生物学会.江苏南京.2001.12.281-185.
[80]王凤雪 王云峰.CELOV载体在人类和动物疾病控制上的应用.中国生物工程杂志.2005.25(1):14-17
[81]陈溥言.禽腺病毒Ⅰ型(鸡胚致死孤儿病毒)在非免疫鸡胚中隐性感染可能已经严重危害到我国疫苗生产.畜牧与兽医.2002.34(6):19
[82]周斌 刘华雷 曹瑞兵 等.污染疫苗的禽腺病毒分离和鉴定.南京农业大学学报.2004.27(3):78-80
[83]Logunov Dlu,Cherenova LV,Shmarov MM,et al.Delivery of secreted placental 1 alkaline phosphatase(SEAP) gene in vitro and in vivo as a component of recombinant avian adenovirus (CELO).Mol Gen Microbiol Virusol.2002,4:21-25.
[84]Cherenova LV,Logunov DY,Shashkova EV,et al.Recombinant avian adenovirus CELO expressing the human interleukin-2:characterization in vitro,in ovo and in vivo.Virus Res.2004Mar 15;100(2):257-61.
[85]Logunov DY,Ilyinskaya GV,Cherenova LV,et al.Restoration of P53 tumor-suppressor acting in human tumor cells in vitro and in their exnografts in vivo by recombinant avian adenovirus CELO-p53.Gene Ther.2004,11(1):79-84.
[86]Shashkova EV,Cherenova LV,Kazansky DB,et al.Avian adenovirus vector CELO-TK displays anticancer activity in human cancer cells and suppresses established murine melanoma tumors.Cancer Gene Ther.2005 Jul;12(7):617-26.
[87]Avdeeva SV,Voronov DA,Khaidarova NV,et al.The CELO-ANG recombinant avian adenovirus with human angiogenine gene inducing neovascularization in the anterior tibial muscle of rat,Mol Gen Mikrobiol Virusol.2004;(4):38-40
[88]Ding H,Wang R,Marcel R,Fisher DZ,et al.Adenovirus-mediated expression of a truncated PDGF beta receptor inhibits thrombosis and neointima formation in an avian arterial injury model.Thromb Haemost.2001 Sep;86(3):914-22
[89]Stevenson M,Boos E,Herbert C,et al.Chick embryo lethal orphan virus can be polymer-coated and retargeted to infect mammalian cells.Gene Ther.2006 Feb;13(4):356-68.
[1]陈溥言.禽腺病毒1型(鸡胚致死孤儿病毒)在非免疫鸡胚中隐性感染可能己经严重危害到我国疫苗生产.畜牧与兽医.2002,34(6):19.
[2]周斌 曹瑞兵 卢银华等.随机引物PCR扩增鸡胚致死孤儿病毒DNA的研究.中国病毒学.2003,18(2):137-140.
[3]Sam blook等著.金冬雁等译.分子克隆(实验指南).科学出版社.1996.第二版
[4]Dhinakar Raj G,Sivakumar S,Matheswaran K,et al.Detection of egg drop syndrome virus antigen or genome by enzyme-linked immunosorbent assay or polymerase chain reaction.Avian Pathol.2003 Oct;32(5):545-50.
[5]Kumar NS,Katafia JM,Koti M,et al.Detection of egg drop syndrome 1976 virus by polymerase chain reaction and study of its persistence in experimentally infected layer birds.Acta Virol.2003;47(3):179-84.
[6]Raj GD,Ratnapraba S,Matheswaran K,et al.Comparison of hemagglutination inhibition test and ELISA in quantification of antibodies to egg drop syndrome virus.Acta V'trol.2004;48(3):183-7.
[7]Schrenzel M,Oaks JL,Rotstein D,et al.Characterization of a new species of adenovirus in falcons.J Clin Microbiol.2005 Jul;43(7):3402-13.
[8]Uhart MM,Quintana F,Karesh WB,et al.Hematology,plasma biochemistry,and serosurvey for selected infectious agents in southern giant petrels from Patagonia,Argentina.J Wildl Dis.2003 Apr;39(2):359-65.
[9]Raue R,Gerlach H,Muller H.Phylogenetic analysis of the hexon loop 1 region of an adenovirus from psittacine birds supports the existence of a new psittaeine adenovirus(PsAdV).Arch Virol.2005Oct;150(10):1933-43.
[10]Hollmen TE,Franson JC,Flint PL,et al.An adenovirus linked to mortality and disease in long-tailed ducks(Clangula hyemalis) in Alaska.Avian Dis.2003 Oct-Dec;47(4):1434-40.
[11]Guy JS,Barnes HJ,Smith L,et al.Partial characterization of an adenovirus-like virus isolated from broiler chickens with transmissible viral proventrieulitis.Avian Dis.2005 Sep;49(3):344-51.
[12]Morikazu Shinagawa,Yoichi Iida,Akihisa,et al.Phylogenetic relationships between adenoviruses as inferred from nucleotide sequences of inverted terminal repeats.Gene,1987,55:85-93.
[13]Michael Hess,Helmut Blocker,Petra Brandt.The Complete Nucleotide Sequence of The Egg Drop Syndrome Virus:An Intermediate between Mastadenoviruses and Aviadenoviruses.Virology,1997,238:145-156.
[14]刘岳龙,徐福州,崔治中等.鹅源腺病毒Y81G4株基因组末端倒置重复序列(ITR)的鉴定和结构分析.中国预防兽医学报.2000,22(4):285-288.
[15]Chiocca S,Kurtev V,Colombo R,et al.Histone deacetylase 1 inactivation by an adenovirus early gene product.Curr Biol,2002,12(7):594-8
[16]Misiurina O,Neugodova G L,Naroditskii B S.Transcription of anti-apoptotic proteins from CELO and EDS avian adenovirus at early stages of infection.Mol Gen Mikrobiol Virusol,2000,(4):15-7
[17]Chiocca S,Baker A,Cotton M.Identification of a novel antippotitic protein,GAM-1,encoded by the CELO adenovirus.J Virol,1997,71(4):3168-77
[18]Hacker D L,Derow E,Wurm F M.The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells.J Biotechnol,2005,117(1):21-9
[1]Szilvia L,Farkas M,Benk P,et al.Genomic and phylogenetic analyses of an adenovirus isolated from a corn snake(Elaphe guttata) imply a common origin with members of the proposed new genus Atadenovirus [J].Journal of General Virology,2002,83:2403-2410.
[2]Duncan J,Mc Geoch,Derek Gat herer.Integrating reptilian herpesviruses into the family herpesviridae[J].Journal of Virology,2005,79(2):725-731.
[3]Fauquet C M,Mayo M A,Maniloff J,et al.Virus Taxonomies 8th Report of ICTV[M].Amsterdaml:Elsevier Academic Press,2005.
[4]富强 翟新验 卢胜明.3Rs原则的应用实例-用鸡胚肝细胞替代鸡肾细胞培养禽腺病毒.实验动物科学与管理.2003.20(3):54-56
[5]殷震 刘景华 主编.动物病毒学(第二版).科学出版社.1997.
[6]B.W.Calnet et al.Disease of Poultry[M].Tenth Edition.1997;607-642
[7]B.W Carlnek,et al.Disease of Poultry,第十版,中国农业出版社,782-783
[8]Schonewille E,Singh A,Gobel TW,et al.Fowl adenovirus(FAdV) serotype 4 causes depletion of B and T cells in lymphoid organs in specific pathogen-free chickens following experimental infection.Vet Immunol Immunopathol.2008.121(1-2):130-9.
[9]甘孟侯.中国禽病学.中国农业出版社.1999,94-104
[10]Cook J.K.A.Pathogenicity of avian adenovirus for day-old chicks.J Comp Pathol,1974,84:505-515
[11]Barr D.A,P.Scott.Adenovirus and IBH.Proc 2~(nd) Asian/Pacific poult health conf[Proc 112].Post Gruduate Comm Vet Sci,University of Sydney,Austrilia,323-326
[12]Yates V J,Rhee Y O,Fry D E.Serological response of chickens exposed to a type 1 avian adenovirus alone or in combination with the adeno-associated virus.Avian Dis.1977,21:408-14.
[1]Chiocca S,Kurzbauer R,Schaffner G,et al.The complete DNA sequence and genomic organization of the avian adenovirus CELO.J Virol.1996 May;70(5):2939-49.
[2]Ojkic D,Nagy E.The complete nucleotide sequence of fowl adenovirus type 8.J Gen Virol.2000 Jul;81(Pt 7):1833-7.
[3]Hess M,Blocker H,Brandt P.The complete nucleotide sequence of the egg drop syndrome virus:an intermediate between mastadenoviruses and aviadenoviruses.Virology.1997 Nov 10;238(1):145-56.
[4]Pitcovski J,Mualem M,Rei-Koren Z,et al.The complete DNA sequence and genome organization of the avian adenovirus,hemorrhagic enteritis virus.Virology.1998 Sep 30;249(2):307-15.
[5]Achille Francois,Nicolas Eterradossi,Bernard Delmas,et al.Construction of Avian Adenovirus CELO Recombinants in Cosmids.J.Virol.,2001,75(11):5288-5301.
[6]Achille Francois,Christophe Chevalier,Bernard Delmas,et al.Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens.Vaccine.2004 Jun 2;22(17-18):2351-60
[7]Sheppard M,W Werner and E Tsatas,et al.Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces pretective immunity against bursal disease.Achives of Virology,1998,143:915-930.
[8]Michael A.Johnson,Cathryn Pooley,John W.Lowenthal.Delivery of avian cytokines by adenovirus vectors.Developmental and Comparative Immunology,2000,24:343-354.
[9]Michael A.Johnson,Catherine Pooley,Jagoda Ignjatovic,et al.A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus.Vaccine,2003,21:2730-2736.
[10]Davor Ojkic,Nagy E.The long repeat region is dispensable for fowl adenovirus replication in vitro.Virology,2001,283(2):197-206.
[11]Davor Ojkic and Eva Nagy.Antibody response and virus tissue distribution in chickens inoculated with wild-type and recombinant fowl adenoviruses.Vaccine,2003,22(1):42-48.
[12]何秀苗 张科 秦爱建等.Ⅰ群禽腺病毒江苏分离株(FA-V-JS)的分离鉴定.中国预防兽医学报. 2005.27(1):42-45
[13]何秀苗 秦爱建 刘岳龙等.禽腺病毒江苏株(JS)复制非必需片段的确定.微生物学报.2004.44(3):23-27
[14]Sam blook等著.金冬雁等译.分子克隆(实验指南).科学出版社.1996.第二版
[15]Addison C L,Hitt M,Kunsken D,et al.Comparison of the human versus murine cytomegalovirus immediate early gene promoters for transgene expression by adenovirus vectors.J Virol.,1997,78(7):1653-1661.
[16]Payet V,Arnauld C,Picault JP,et al.Transcriptional organization of the avian adenovirus CELO.J Virol.1998 Nov;72(11):9278-85.
[17]Sheppard M,Werner W,McCoy RJ,et al.The major late promoter and bipartite leader sequence of fowl adenovirus.Arch Virol.1998;143(3):537-48.
[18]Leong K,Lee W,Berk AJ(1990) High-level transcription from the adenovirus major late promoter requires downstream binding sites for late-phase-specific factors.J Virol 64:51-60
[19]Li X-C,Huang WL,Flint SJ(1992) The downstream regulatory sequence of the adenovirus type 2 major late promoter is fuctionally redundant.J Virol 66:5 685-5 690
[20]Mondesert G,Kedinger C(1991) Cooperation between upstream and downstream elements of the adenovirus major late promoter for maximal late phase-specific transcription.Nucleic Acids Res 19:3 221-3228
[1]Yates V J,Rhee Y O,Fry D E.Serological response of chickens exposed to a type 1 avian adenovirus alone or in combination with the adeno-associated virus.Avian Dis.,1977,21:408-414.
[2]Cherenova LV,Logunov DY,Shashkova EV,et al.Recombinant avian adenovirus CELO expressing the human interleukin-2:characterization in vitro,in ovo and in vivo.Virus Res.2004 Mar 15;100(2):257-61.[3]Francois A,Eterradossi N,Delmas B,et al.Construction of avian adenovirus CELO recombinants in cosmids.J Virol.2001 Jun;75(11):5288-301.
[4]Francois A,Chevalier C,Delmas B,et al.Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens.Vaccine.2004 Jun 2;22(17-18):2351-60.
[5]Alexander HS,Huber P,Cao J,et al.Growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line.J Virol Methods.1998 Sep;74(1):9-14.
[6]Scholz E,Welniak E,Nyholm T,et al.An avian hepatoma cell line for the cultivation of infectious laryngotracheitis virus and for the expression of foreign genes with a mammalian promoter.J Virol Methods.1993 Aug;43(3):273-86.
[7]Payet V,Arnauld C,Picault JP,et al.Transcriptional organization of the avian adenovirus CELO.J Virol.1998 Nov;72(11):9278-85.
[8]Sheppard M,Werner W,McCoy RJ,et al.The major late promoter and bipartite leader sequence of fowl adenovirus.Arch Virol.1998;143(3):537-48.
[9]Tsukamoto K,Saito S,Saeki S,et al.Complete long lasting protection against lethal infectious bursal disease virus challenge by a single vaccination with an avian herpesvirus vector expressing VP2 antigens[J].J Virol,2002,76(11):5637-45.
[10]Sheppard M,W Wemer and E Tsatas,et al.Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease.Achives of Virology,1998,143:915-930.
[11]Michael A.Johnson,Cathryn Pooley,John W.Lowenthal.Delivery of avian cytokines by adenovirus vectors.Developmental and Comparative Immunology,2000,24:343-354.
[12]Michael A.Johnson,Catherine Pooley,Jagoda Ignjatovic,et al.A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus.Vaccine,2003,21:2730-2736.