丹参、藜芦对大鼠MSCs分化为神经元样细胞及SMNmRNA表达的影响
|
摘要
目的:体外分离、培养大鼠骨髓间充质干细胞(Mesenchymal stem cells,MSCs),研究MSCs生物学特性。观察丹参、藜芦水煎液诱导MSCs向神经元样细胞分化的作用,研究丹参、藜芦水煎液对分化后神经元样细胞SMNmRNA表达的影响;并采用血清药理学方法,研究丹参、藜芦含药血清诱导MSCs分化为神经元样细胞的作用。探索提高MSCs向神经元样细胞分化和上调分化后神经元样细胞SMNmRNA表达的干预因素,为进行神经元样细胞移植治疗脊髓。性肌萎缩症提供基础。
方法:
1.采用全骨髓贴壁法分离培养MSCs;通过形态学观察、绘制生长曲线、流式细胞仪进行MSCs表面抗原鉴定和检测MSCs生长周期来鉴定MSCs;建立体外培养的稳定MSCs细胞株系。
2.观察丹参、藜芦水煎液诱导MSCs向神经元样细胞分化的作用,免疫组化法检测神经元样细胞Nestin、NSE、GFAP,摸索β-巯基乙醇、丹参水煎液、藜芦水煎液、丹参+藜芦水煎液的最佳诱导浓度,观察各组最佳浓度诱导MSCs向神经元样细胞分化的作用。
3.观察丹参、藜芦水煎液对分化后神经元样细胞SMNmRNA表达的影响,FQ-PCR法检测空白组、β-巯基乙醇诱导组、丹参水煎液组、藜芦丹参水煎液组、丹参+藜芦水煎液组对分化后神经元样细胞SMNmRNA表达的影响。
4.观察丹参、藜芦含药血清诱导MSCs向神经元样细胞分化的作用,免疫组化法检测神经元样细胞Nestin、NSE、GFAP,摸索丹参含药血清、藜芦含药血清、丹参+藜芦含药血清的最佳诱导浓度,观察各组最佳浓度诱导MSCs向神经元样细胞分化的作用。
结果:
1.全骨髓贴壁法分离培养的MSCs具有均质性好、增殖活力强、传代生长快的特点。本实验培养的MSCs经流式细胞仪检测,表达整合素家族成员的CD29和粘附分子CD44,不表达泛白细胞标志CD45;MSCs处于G_0-G_1期的细胞为87.63%,G_2-M期的细胞为3.57%,S期的细胞为8.80%:生长曲线均呈S形,接种1-3 d细胞的OD值变化不大,到第3 d细胞数目明显增加,第8 d到达顶点,之后减少;证实已成功建立稳定的MSCs株系。
2.β-巯基乙醇、丹参水煎液、藜芦水煎液和丹参+藜芦水煎液最佳诱导MSCs向神经元样细胞分化浓度分别是β-巯基乙醇1mmol/L的无血清L-DMEM培养液、丹参水煎液120μl/ml10%胎牛血清L-DMEM培养液、藜芦水煎液140μl/ml 10%胎牛血清L-DMEM培养液和丹参+藜芦80μl/ml 10%胎牛血清L-DMEM培养液。空白组Nestin、NSE和GFAP表达均为阴性。丹参+藜芦水煎液组Ncstin阳性细胞率高于β-巯基乙醇组,P＜0.01;丹参+藜芦水煎液组NSE阳性细胞率高于β-巯基乙醇诱导组,P＜0.05;丹参+藜芦水煎液组Nestin、NSE阳性细胞率分别高于丹参水煎液和藜芦水煎液,P＜0.05;各实验组GFAP表达均为阴性。
3.β-巯基乙醇组、丹参水煎液组、藜芦水煎液组、丹参+藜芦水煎液组分化后神经元样细胞SMNmRNA表达丰度值均高于空白组,P＜0.01;丹参+藜芦水煎液组SMNmRNA表达丰度值高于β-巯基乙醇组,P＜0.05:丹参+藜芦水煎液组SMNmRNA表达丰度值高于丹参水煎液组、藜芦水煎液组,P＜0.01;β-巯基乙醇组SMNmRNA表达丰度值高于丹参水煎液组、藜芦水煎液组,P＜0.05。
4.丹参含药血清组、藜芦含药血清和丹参+藜芦含药血清最佳诱导MSCs向神经元样细胞分化浓度分别是丹参含药血清20μl/ml 10%胎牛血清L-DMEM培养液、藜芦含药血清25μl/ml 10%胎牛血清L-DMEM培养液和丹参+藜芦含药血清15μl/ml 10%胎牛血清L-DMEM培养液。空白组Ncstin、NSE和GFAP表达均为阴性。丹参+藜芦含药血清诱导组Nestin阳性细胞率高于β-巯基乙醇诱导组,P＜0.05;丹参+藜芦诱导组NSE阳性细胞率高于β-巯基乙醇诱导组,P＜0.01;各实验组GFAP表达均为阴性。
结论:
1.运用全骨髓贴壁法,可以建立体外稳定培养的MSCs株系。
2.丹参水煎液、丹参+藜芦水煎液可以诱导体外培养的MSCs向神经元样细胞分化,藜芦水煎液诱导MSCs向神经元样细胞分化作用不明显,丹参+藜芦水煎液诱导MSCs向神经元.样细胞分化作用高于其它诱导组,MSCs没有诱导因素的作用不会向神经元样细胞分化。
3.丹参、藜芦水煎液可以促进分化后神经元样细胞表达SMNmRNA,丹参+藜芦水煎液对神经元样细胞SMNmRNA表达作用强于丹参水煎液或藜芦水煎液,分化后的神经元样细胞在丹参+藜芦水煎液干预下有向运动神经元细胞进一步分化的趋向。
4.丹参+藜芦含药血清组促MSCs向神经元样细胞分化作用弱于丹参+藜芦水煎液组,原因可能是丹参+藜芦组水煎液经过大鼠机体代谢,含药血清中促MSCs向神经元样细胞分化作用的有效组份(群)降低所致。
Objective:Mesenchymal stem cells(MSCs) were isolated from the Spragu-Dawley rats and cμlutred in vitro,then to study the biological characteristics of MSCs.Compairing the effects of that MSCs were dieffrentiated into neuron-like cells,which inducing by the decoction and drug-containing serum of Salvia Mitiorrhiza,Veratrum nigrum.To investigate the expression of SMNmRNA in neuron-like cells,which inducing by the decoction Salvia Mitiorrhiza and Veratrum nigrum,and to analyse the mechanism of them.To explore the ideal factors which can elevate the differentiation percentage of MSCs dieffrentiated into neuron-like cells,and also to explore the ideal condition which can enhance the expression of SMNmRNA in neuron-like cells.It might offer reference for transplanting neuron-like cells to treat spinalm uscμlarat rophy(SMA).
Methods:
1.To investigate the effects of MSCs were isolated from the rats by means of the density-gradient centrifugation and the differential time adherent method.The stable and uniformal MSCs were instaurating in vitro.To observe the morphologic characteristics of MSCs,MTT method was used to investigate the proliferation activity of MSCs,the growth curve of MSCs was drawing according as proliferation activity of MSCs.The cell surface antigens and Cell cycle of MSCs were detected by flow cytometry(FCM).
2.The effects of that MSCs were dieffrentiated into neuron-like cells induced by control group, classic group,Salvia Mitiorrhiza,Veratrum nigrum,Salvia Mitiorrhiza and Veratrum nigrum,was investigated,To explore the most effective concentration of the above groups on differentiation MSCs into neuron-like cells.Neuron-specific enolase(NSE),Nestin,gliall fibrillary acaidic protein(GFAP) of neuron-like cells were detected by immunocyto chemistry.The morphologic characteristics and the proliferation of neuron-like cells was observed.
3.FQ-PCR was used to detect the expression of SMNmRNA in neuron-like cells,which inducing by control group,classic group,Salvia Mitiorrhiza decoction,Veratrum nigrum decoction, Salvia Mitiorrhiza and Veratrum nigrum decoction,To analyse expression of SMNmRNA in the above groups.
4.To prepare different concentration serum and drug-containing serum(control group serum, Salvia Mitiorrhiza serum,Veratrum nigrum serum,Salvia Mitiorrhiza and Veratrum nigrum serum).To explore the the most effective concentration of the above groups on differentiation MSCs into neuron-like cells.NSE,GFAP,GFAP of neuron-like cells were detected by immunocyto chemistry.The morphologic characteristics and the proliferation of neuron-like cells was observed.
Results:
1.The MSCs isolated with differential time adherent method has characteristic of high homogenize,proliferating actively and differentiating quickly.The results of the surface antigens of MSCs detected by flow cytometer showed the positive expressions of CD29 and CD44 and the negative expressions of CD45,It is accord with the immunological characteristic of MSCs, It's also substantiated that MSCs coμld be obtained and purified in vitro by our experimentation..
2.The most effective differentiation concentration ofβ-mercaptoethanol(β-ME),Salvia Mitiorrhiza,Veratrum nigrum,Salvia Mitiorrhiza and Veratrum nigrum were L-DMEM cμlture medium containing 1mmol/1β-mercaptoethanol,L-DMEM cμlture medium containing 10% fetal calf seurm(FCS) and 120μl/ml Salvia Mitiorrhiza,L-DMEM cμlture medium containing 10%fetal calf seurm(FCS) and 140μl/ml Veratrum nigrum,L-DMEM cμlture medium containing 10%FCS and 80μl/ml Salvia Mitiorrhiza and Veratrum nigrum respectively.The induced expression rate of NSE and Nestin in the induced groups was higher than that in the control group,P＜0.01;The expression rate of Nestin in Salvia Mitiorrhiza and Veratrum nigrum was higher than that inβ-ME;The expression rate of NSE in Salvia Mitiorrhiza and Veratrum nigrum was higher than that inβ-ME,P＜0.05;but negatively with GFAP.
3.The most effective differentiation concentration of Salvia Mitiorrhiza serum,Veratrurn nigrum serum,Salvia Mitiorrhiza and Veratrum nigrum serum were L-DMEM cμlture medium containing 10%fetal calf seurm(FCS) and 20μl/ml Salvia Mitiorrhiza serum,L-DMEM cμlture medium containing 10%fetal calf seurm(FCS) and 25μl/ml Veratrum nigrum serum,L-DMEM cμlture medium containing 10%FCS and 15μl/ml Salvia Mitiorrhiza and Veratrum nigrum serum respectively.The induced expression rate of NSE and Nestin in the induced groups was higher than that in the control group,P＜0.05;The expression rate of Nestin in Salvia Mitiorrhiza and Veratrum nigrum serum was higher than that inβ-ME;The expression rate of NSE in Salvia Mitiorrhiza and Veratrum nigrum serum was higher than that inβ-ME,P＜0.01;but negatively with GFAP.
4.The relative expression abundance of SMNmRNA inβ-ME,Salvia Mitiorrhiza,Veratrum nigrum,Salvia Mitiorrhiza and Veratrum nigrum was higher than that in control group,P＜0.01.The relative expression abundance of SMNmRNA in Salvia Mitiorrhiza and Veratrum nigrum was higher than that inβ-ME,P＜0.05.
Conclusion:
1.The differential time adherent method was a ideal method to isolate MSCs from Spragu-Dawley rat.
2.Salvia Mitiorrhiza decoction,Salvia Mitiorrhiza and Veratrum nigrum decoction coμld induce MSCs to differentiate into neuron-like cells in vitro.The effects of that MSCs were dieffrentiated into neuron-like cells induced byeratrum nigrum decoction was indistinctively. The differentiation effects of Salvia Mitiorrhiza and Veratrum nigrum decoction was higher than that in other groups,The reasons might be that there were some combination components and effectiveness in the Salvia Mitiorrhiza and Veratrum nigrum decoction,the contrary integration effects of Salvia Mitiorrhiza and Veratrum nigrum decoction need to be explore profoundly.
3.The relative expression abundance of SMNmRNA in Salvia Mitiorrhiza and Veratrurn nigrum decoction was higher than that in other groups.The reasons might be that the SMN gene was in state of silencing,there was no transcription and expression of SMNmRNA.It's might be the components and effectiveness in Salvia Mitiorrhiza and Veratrum nigrum decoction that activated the SMN gene of neuron-like cells,and enhanced the expression of SMNmRNA.
4.The differentiation effects of Salvia Mitiorrhiza and Veratrum nigrum serum was lower than that in Salvia Mitiorrhiza and Veratrum nigrum decoction.The reasons might be that the induced components and effectiveness in Salvia Mitiorrhiza and Veratrum nigrum decoction were weaken by through the metabolism of rats.
方法:
1.采用全骨髓贴壁法分离培养MSCs;通过形态学观察、绘制生长曲线、流式细胞仪进行MSCs表面抗原鉴定和检测MSCs生长周期来鉴定MSCs;建立体外培养的稳定MSCs细胞株系。
2.观察丹参、藜芦水煎液诱导MSCs向神经元样细胞分化的作用,免疫组化法检测神经元样细胞Nestin、NSE、GFAP,摸索β-巯基乙醇、丹参水煎液、藜芦水煎液、丹参+藜芦水煎液的最佳诱导浓度,观察各组最佳浓度诱导MSCs向神经元样细胞分化的作用。
3.观察丹参、藜芦水煎液对分化后神经元样细胞SMNmRNA表达的影响,FQ-PCR法检测空白组、β-巯基乙醇诱导组、丹参水煎液组、藜芦丹参水煎液组、丹参+藜芦水煎液组对分化后神经元样细胞SMNmRNA表达的影响。
4.观察丹参、藜芦含药血清诱导MSCs向神经元样细胞分化的作用,免疫组化法检测神经元样细胞Nestin、NSE、GFAP,摸索丹参含药血清、藜芦含药血清、丹参+藜芦含药血清的最佳诱导浓度,观察各组最佳浓度诱导MSCs向神经元样细胞分化的作用。
结果:
1.全骨髓贴壁法分离培养的MSCs具有均质性好、增殖活力强、传代生长快的特点。本实验培养的MSCs经流式细胞仪检测,表达整合素家族成员的CD29和粘附分子CD44,不表达泛白细胞标志CD45;MSCs处于G_0-G_1期的细胞为87.63%,G_2-M期的细胞为3.57%,S期的细胞为8.80%:生长曲线均呈S形,接种1-3 d细胞的OD值变化不大,到第3 d细胞数目明显增加,第8 d到达顶点,之后减少;证实已成功建立稳定的MSCs株系。
2.β-巯基乙醇、丹参水煎液、藜芦水煎液和丹参+藜芦水煎液最佳诱导MSCs向神经元样细胞分化浓度分别是β-巯基乙醇1mmol/L的无血清L-DMEM培养液、丹参水煎液120μl/ml10%胎牛血清L-DMEM培养液、藜芦水煎液140μl/ml 10%胎牛血清L-DMEM培养液和丹参+藜芦80μl/ml 10%胎牛血清L-DMEM培养液。空白组Nestin、NSE和GFAP表达均为阴性。丹参+藜芦水煎液组Ncstin阳性细胞率高于β-巯基乙醇组,P＜0.01;丹参+藜芦水煎液组NSE阳性细胞率高于β-巯基乙醇诱导组,P＜0.05;丹参+藜芦水煎液组Nestin、NSE阳性细胞率分别高于丹参水煎液和藜芦水煎液,P＜0.05;各实验组GFAP表达均为阴性。
3.β-巯基乙醇组、丹参水煎液组、藜芦水煎液组、丹参+藜芦水煎液组分化后神经元样细胞SMNmRNA表达丰度值均高于空白组,P＜0.01;丹参+藜芦水煎液组SMNmRNA表达丰度值高于β-巯基乙醇组,P＜0.05:丹参+藜芦水煎液组SMNmRNA表达丰度值高于丹参水煎液组、藜芦水煎液组,P＜0.01;β-巯基乙醇组SMNmRNA表达丰度值高于丹参水煎液组、藜芦水煎液组,P＜0.05。
4.丹参含药血清组、藜芦含药血清和丹参+藜芦含药血清最佳诱导MSCs向神经元样细胞分化浓度分别是丹参含药血清20μl/ml 10%胎牛血清L-DMEM培养液、藜芦含药血清25μl/ml 10%胎牛血清L-DMEM培养液和丹参+藜芦含药血清15μl/ml 10%胎牛血清L-DMEM培养液。空白组Ncstin、NSE和GFAP表达均为阴性。丹参+藜芦含药血清诱导组Nestin阳性细胞率高于β-巯基乙醇诱导组,P＜0.05;丹参+藜芦诱导组NSE阳性细胞率高于β-巯基乙醇诱导组,P＜0.01;各实验组GFAP表达均为阴性。
结论:
1.运用全骨髓贴壁法,可以建立体外稳定培养的MSCs株系。
2.丹参水煎液、丹参+藜芦水煎液可以诱导体外培养的MSCs向神经元样细胞分化,藜芦水煎液诱导MSCs向神经元样细胞分化作用不明显,丹参+藜芦水煎液诱导MSCs向神经元.样细胞分化作用高于其它诱导组,MSCs没有诱导因素的作用不会向神经元样细胞分化。
3.丹参、藜芦水煎液可以促进分化后神经元样细胞表达SMNmRNA,丹参+藜芦水煎液对神经元样细胞SMNmRNA表达作用强于丹参水煎液或藜芦水煎液,分化后的神经元样细胞在丹参+藜芦水煎液干预下有向运动神经元细胞进一步分化的趋向。
4.丹参+藜芦含药血清组促MSCs向神经元样细胞分化作用弱于丹参+藜芦水煎液组,原因可能是丹参+藜芦组水煎液经过大鼠机体代谢,含药血清中促MSCs向神经元样细胞分化作用的有效组份(群)降低所致。
Objective:Mesenchymal stem cells(MSCs) were isolated from the Spragu-Dawley rats and cμlutred in vitro,then to study the biological characteristics of MSCs.Compairing the effects of that MSCs were dieffrentiated into neuron-like cells,which inducing by the decoction and drug-containing serum of Salvia Mitiorrhiza,Veratrum nigrum.To investigate the expression of SMNmRNA in neuron-like cells,which inducing by the decoction Salvia Mitiorrhiza and Veratrum nigrum,and to analyse the mechanism of them.To explore the ideal factors which can elevate the differentiation percentage of MSCs dieffrentiated into neuron-like cells,and also to explore the ideal condition which can enhance the expression of SMNmRNA in neuron-like cells.It might offer reference for transplanting neuron-like cells to treat spinalm uscμlarat rophy(SMA).
Methods:
1.To investigate the effects of MSCs were isolated from the rats by means of the density-gradient centrifugation and the differential time adherent method.The stable and uniformal MSCs were instaurating in vitro.To observe the morphologic characteristics of MSCs,MTT method was used to investigate the proliferation activity of MSCs,the growth curve of MSCs was drawing according as proliferation activity of MSCs.The cell surface antigens and Cell cycle of MSCs were detected by flow cytometry(FCM).
2.The effects of that MSCs were dieffrentiated into neuron-like cells induced by control group, classic group,Salvia Mitiorrhiza,Veratrum nigrum,Salvia Mitiorrhiza and Veratrum nigrum,was investigated,To explore the most effective concentration of the above groups on differentiation MSCs into neuron-like cells.Neuron-specific enolase(NSE),Nestin,gliall fibrillary acaidic protein(GFAP) of neuron-like cells were detected by immunocyto chemistry.The morphologic characteristics and the proliferation of neuron-like cells was observed.
3.FQ-PCR was used to detect the expression of SMNmRNA in neuron-like cells,which inducing by control group,classic group,Salvia Mitiorrhiza decoction,Veratrum nigrum decoction, Salvia Mitiorrhiza and Veratrum nigrum decoction,To analyse expression of SMNmRNA in the above groups.
4.To prepare different concentration serum and drug-containing serum(control group serum, Salvia Mitiorrhiza serum,Veratrum nigrum serum,Salvia Mitiorrhiza and Veratrum nigrum serum).To explore the the most effective concentration of the above groups on differentiation MSCs into neuron-like cells.NSE,GFAP,GFAP of neuron-like cells were detected by immunocyto chemistry.The morphologic characteristics and the proliferation of neuron-like cells was observed.
Results:
1.The MSCs isolated with differential time adherent method has characteristic of high homogenize,proliferating actively and differentiating quickly.The results of the surface antigens of MSCs detected by flow cytometer showed the positive expressions of CD29 and CD44 and the negative expressions of CD45,It is accord with the immunological characteristic of MSCs, It's also substantiated that MSCs coμld be obtained and purified in vitro by our experimentation..
2.The most effective differentiation concentration ofβ-mercaptoethanol(β-ME),Salvia Mitiorrhiza,Veratrum nigrum,Salvia Mitiorrhiza and Veratrum nigrum were L-DMEM cμlture medium containing 1mmol/1β-mercaptoethanol,L-DMEM cμlture medium containing 10% fetal calf seurm(FCS) and 120μl/ml Salvia Mitiorrhiza,L-DMEM cμlture medium containing 10%fetal calf seurm(FCS) and 140μl/ml Veratrum nigrum,L-DMEM cμlture medium containing 10%FCS and 80μl/ml Salvia Mitiorrhiza and Veratrum nigrum respectively.The induced expression rate of NSE and Nestin in the induced groups was higher than that in the control group,P＜0.01;The expression rate of Nestin in Salvia Mitiorrhiza and Veratrum nigrum was higher than that inβ-ME;The expression rate of NSE in Salvia Mitiorrhiza and Veratrum nigrum was higher than that inβ-ME,P＜0.05;but negatively with GFAP.
3.The most effective differentiation concentration of Salvia Mitiorrhiza serum,Veratrurn nigrum serum,Salvia Mitiorrhiza and Veratrum nigrum serum were L-DMEM cμlture medium containing 10%fetal calf seurm(FCS) and 20μl/ml Salvia Mitiorrhiza serum,L-DMEM cμlture medium containing 10%fetal calf seurm(FCS) and 25μl/ml Veratrum nigrum serum,L-DMEM cμlture medium containing 10%FCS and 15μl/ml Salvia Mitiorrhiza and Veratrum nigrum serum respectively.The induced expression rate of NSE and Nestin in the induced groups was higher than that in the control group,P＜0.05;The expression rate of Nestin in Salvia Mitiorrhiza and Veratrum nigrum serum was higher than that inβ-ME;The expression rate of NSE in Salvia Mitiorrhiza and Veratrum nigrum serum was higher than that inβ-ME,P＜0.01;but negatively with GFAP.
4.The relative expression abundance of SMNmRNA inβ-ME,Salvia Mitiorrhiza,Veratrum nigrum,Salvia Mitiorrhiza and Veratrum nigrum was higher than that in control group,P＜0.01.The relative expression abundance of SMNmRNA in Salvia Mitiorrhiza and Veratrum nigrum was higher than that inβ-ME,P＜0.05.
Conclusion:
1.The differential time adherent method was a ideal method to isolate MSCs from Spragu-Dawley rat.
2.Salvia Mitiorrhiza decoction,Salvia Mitiorrhiza and Veratrum nigrum decoction coμld induce MSCs to differentiate into neuron-like cells in vitro.The effects of that MSCs were dieffrentiated into neuron-like cells induced byeratrum nigrum decoction was indistinctively. The differentiation effects of Salvia Mitiorrhiza and Veratrum nigrum decoction was higher than that in other groups,The reasons might be that there were some combination components and effectiveness in the Salvia Mitiorrhiza and Veratrum nigrum decoction,the contrary integration effects of Salvia Mitiorrhiza and Veratrum nigrum decoction need to be explore profoundly.
3.The relative expression abundance of SMNmRNA in Salvia Mitiorrhiza and Veratrurn nigrum decoction was higher than that in other groups.The reasons might be that the SMN gene was in state of silencing,there was no transcription and expression of SMNmRNA.It's might be the components and effectiveness in Salvia Mitiorrhiza and Veratrum nigrum decoction that activated the SMN gene of neuron-like cells,and enhanced the expression of SMNmRNA.
4.The differentiation effects of Salvia Mitiorrhiza and Veratrum nigrum serum was lower than that in Salvia Mitiorrhiza and Veratrum nigrum decoction.The reasons might be that the induced components and effectiveness in Salvia Mitiorrhiza and Veratrum nigrum decoction were weaken by through the metabolism of rats.
引文
1.Young PJ,Day PM,Zhou J,et al.A direct interaction between the survival motor neuron protein and p53 and its relationship to spinal muscular atrophy[J].Biol Chem,2002,277(4):2852-2859
2.Pittenger MF,Mackay AM,Jaiswal SC,et al.Multilineage potential Of adult human mesenchymal stem cell[J].Science,1999,284(5411):143.
3.Woodbury D,Schwarz EJ,Prockop DJ,et al.Adult rat and human bone marrow stromal cells differentiatein to neurons[J].J Neurosci Res,2000,61(4):364.
4.胡向阳,张荣华.中药诱导骨髓间充质干细胞向神经样细胞分化研究现状与探讨[J].辽宁中医杂志,2007,34(11):1657-1658.
5.张本斯,王凡,邓力.大鼠骨髓间充质干细胞分离培养及表型和功能特点[J].四川大学学报(医学版),2003,34(4):738-741.
6.Friedenstein AJ,Gorskaja JF,Kulagina NN.Fibroblast precursors in normal and irradiated mouse hematopoietic organs[J].Exp Hematol.1976,4(5):267-274.
7.Castro Malaspina H,Gay RE,gesnick G.et al.Characterization of human bone marrow fibroblast colony-forming cells and their progeny[J].Blood,1980,56:289-301.
8.Conget PA,Minguell JJ.Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells[j].Cell Physiol,1999,181:67-73.
9.Friedenstein AJ.Osteogenic stem cells in the bone marrow.Bone and Mineral Research[J],1990,7:243-272.
10.Prockop DJ.Marrow stromal cells as stem cells of nonhematopoietic tissues for continual renewal and as potential vectors for gene therapy[J].Cell Biochem Suppl,1998,30-31:284-285.
11.Deans RJ,Moseley AB.Mesenchymal stem cells:biology and potential clinical uses[J].Exp Hematol.2000,28(8):875-884.
12.Noth U,Full R,Osyczka A M,et al.In vitro engineered cartilage constructs produced by presscoating bio-degradable polymer with human mesenchymal stem cells[J].Tissue Eng,2002,8(1):131-144.
13.Friedenstein A J.Osteogenic stem cells in the bone marrow[J].Bone and Mineral Research,1990,7:243-272.
14.J.E.Dennis,J.P.Carbillet,A.I.Caplan,et al.TheSTRO-1+ marrow cell population is multipotential[J].Cells Tissues Organs,2002,170:73-82.
15.Tondreau T,Lagneaux L,Dejeneffe M,et al.Isolation of mesenchymal stem cells by plastic adhesion or negative selection:phenotype,proliferation kinetics and differentiation potential.[J].Cytotherapy,2004,6(4):372-379.
16.Mizobe T,Kidoguchi K,Tamaki M,et al.Sca-1 and Thy-1 accelerate neuron-like differentiation in bone marrow stromal cells[J].Kobe J Med Sci,2006,52(5):119-125.
17.Quirici N,Soligo D,Bossolasco P,et al.Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factorreceptor antibodies[J].Exp Hematol,2002,30(7):783-789.
18.Gurevich O,Vexler A,Marx G,et al.Fibrin Microbeads for Isolating and growing bone marrow-derived progenitor cells capable of forming bone Tissue[J].Tissue Eng,2002,8(4):661-672.
19.Baddoo M,Hill K.Willinson R,et.al.Characterization of mesenchymal stem cells isolated from murine bone marrow by negative Selection.[J].Cell Biochem,2003,89(6):1235-1249.
20.Zvailer NJ,Man'nova ML,Adams G,et al.Mesenchymal precursor cells in the blood of normal individuals[J].Arthritis Res,2000,2(6):477-479.
21.Erices A,Conger P,Minsuell JJ.Mesenchymal progenitor cells in human umbilical cord blood[J].Br J Haematol,2000,109(1):235-237.
22.Zuk PA,Zhu M,Mizuno H,et al.Multi—lineage cells human adipose tissue:implications for cell based therapies[J].Tissue Eng,2001,7(2):211-213.
23.Kern S,Eichler H,Stoeve J,et al.Comparative analysis of mesenchymal stem cells from bone marrow,umbilical cord blood,or adipose tissue[J].Stem Cells,2006,24(5):1294-1301.
24.Neort WA,KmissdbrinkAB,AnkerPS,etal.Mesenchyreal stem cells promote engraftment of human umbilical cord blood derived CD34 cells in NOD/SCID mice[J].Exp Hematol,2002,30(8):870.
25.Bang Y,Vaessen B,Lenvi KT.et al.Multi—potent progenitor cells can bc isolated from post natal marine bone marrow,muscle,and brain[J]..Exp Hematol,2002,30(8):896.
26.Almeida PG,EIShabrawy D,Porada C,et al.Diferentiative potential of human metanephric mesenchymal cells[J]..Exp Hematol,2002,30(12):1454.
27.Bruder SP,Jaiswal N,Haynesworth SE,et al.Growth kineties,self-renewal,and the osteogenie potential of purified human mesenehymal stem cells during extensive subeultivation following cryoprcservation[J].Cell Bio chem,2003,64(2):278-294.
28.Lennon DP,Haynesworth SE,Young RG,et al.A chemically defined medium supports in vitro Proliferation and maintains the osteochondral potential of rat marrow derived mesenehyma Istem cells[J]..Experimental Cell Research,2005,219(1):211-222.
29.Tsutsumi S,Noel B,Flautre B,et al.Assoeiation of porous hydroxyapapite and bone marrow cells for bone regeneration[J].Bone,1999,25(3):515-545.
30.李建华,黄建荣,樊雪萍,等.胰岛素样生长因子-I对人骨髓间充质干细胞增殖的影响[J].中国临床康复,2004,8(8):1464-1466.
31.杨莉,王冬梅,洪欣,等.成人骨髓间充质干细胞体外扩增和定向诱导分化为骨和软骨细胞的研究[J].自然科学进展,2001,10:25-42.
32.Friedemten AJ,Dglllllo JF,Kulagina NN.et al.Precursors for fibroblast in different population Of hematopoitie cells as deteced by the in vitro colony assay method[J].Exp Hemat,1974,2(2):83-92.
33.姚晓黎,张成,卢锡林等.成人骨髓间质干细胞体外分化为神经元样细胞[J].广东医学,2004,25(5):504-506.
34.JIANG Y,JAHAGIRDAR BN,REINHARDT RL.Pluripoteney of mesenchymal stem cells derived from adult marrow[J].Nature,2002,418(6893):41-49.
35.Conger PA,Minguell JJ.Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells[J].Cell Physiol,1999,181:67-73.
36.路艳蒙,傅文玉,朴英杰.人骨髓间充质干细胞的超微结构[J].电子显微学报,2002,04:373-374.
37.李善宗,张成,吴金浪,等.体外培养大鼠骨髓间质干细胞的超微结构[J].中山大学学报(医学科学版),2003,24(1):11-15.
38.Song L,Tuan RS.Transdifferentiation potential of human mesenchymal stem cells derived from bone marrow[J].FASEBJ,2004,18(9):980-982.
39.Bruder SP,Jaiswal N,Haynesworth SE.Growth kinetics,self-renewal,and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation.[J].Cell Biochem,2005,64(2):278-94.
40.Bianchi G,Banfi A,Mastrogiacomo M,Notaro R,et al.Ex vivo enrichment of mesenehymal cell progenitors by fibroblast growth factor 2[J].Exp.Cell Res,2003,287:98-105
41.Simonsen JL,Rosada C,Serakinei N,et al.Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrowstromal cells[J].Nat Biotectmol,2002,20:592-596.
42.Shi S,Gronthos S,Chen S,et al.Bone formation by human postnatal bone marrow stromal stem cells is enhanced by telomerase expression[J].Nature Biotechnol.2002,20:587-591.
43.D.Baksh,L.Song,R.S.Tuan.Adult mesenchymal stem cells:characterization,differentiation,and application in cell and gene therapy[J].JCell Mol Med,2004,8(3):301-316.
44.Lange C,Bruns H,Kluth D,et ai.Hepato-cytic differentiation of mesenchymal stem cells in coc-ultures with fetal liver cells[J].World J Gastroenterol,2006,12(15):2394-2397.
45.Chen LB,Jiang XB,Yang L.Differentiation of rat marrow mesenehymal stem cells into pancreatic islet betacells[J].World J Gastroenterol,2004,15,10(20):3016-3020.
46.Wislet-Gendebien S,Hans G.Leprince P,et al.Plastieity of cultured mesenchymai stem cells:switch from nestin-positive to excitable neuron-like phenotype[J].Stem Cells,2005,23(3):392-402.
47.裴雪涛.干细胞技术[M].化学工业出版社,2002,第一版:36.
48.Chopp M,Zhang XH,Li Y,et al.Spinal cord injury in rat:Treatment with bone marrow stromal cell transplantation[J].Neuroreport,2000,11(13):3001-3005.
49.Jiang W,Ma A,Wang T,et al.Homing and differentiation ofmesenchymal stem cells delivered intravenously to ischemic myocardium in vivo:a timeseries study.P flugers[J].Arch,2006,453(1):43-52.
50.Koji Natsu,Mitsuo Ochi,Yu Mochizuki,et al.allogeneie bone-derived mesenchymal stromal cells promote the regeneration of injured skeletal muscle without differentiation into myofibers,tissue[J],engineering,2004,10:1093-1099.
51.Chen J,Li Y,Wang L,et ai.Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats[J].Stroke,2001,2(4):1005-1011.
52.LeBlanc K.Mesenchymal stromal cells:Tissue repair and immune modulation[J].Cyto therapy,2006,8(6):559-561.
53.Tse WT,Pendleton JD,Beyer WM,et al.Suppression of logeneic T-cell proliferation by human marrow stromal cells:implications in transplantation[J].Transplantatien,2003,75:389-397.
54.McIntosh KR,Bartholomew A.Stromal cell modulation of the immune system:a potential role for mesenchymal stem cells[J].Graft,2000,3:324-328.
55.Corcione A,Benvenuto F,Ferretti E.Human mesenchymal stem cells modulate B cell functions[J].Blood,2006,107(1):367-372.
56.Le Blanc K,Tammik L,Sundberg B,et al.Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures andmitogenic responses independently of the major histocompatibility complex[J].Scand J Immunol,2003,7:11-20.
57.Di Nicola M,Carlo-Stella C,Magni M,et al.Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli[J]..Blood,2002,99:3838-3843.
58.WEXLER SA,DNALDSON C,DENNING KENDAII F,et al.Adult bone marrow is a rich source of human mesenchymal stem cells but umbilical cord and mobilized adult blood are not.[J].Haematol,2003,121:368-374.
59.MINGUEII JJ,ERICES A,CONGET P..Mesenehymal.stem cells[J]..Exp Biotrices Meal,2001,226:507-520.
60.Minguell,Alejandro Edces,Paulette Conget.Mesenchymal Stem Cells[J].Experimental Biology and Medicine,2001,226:507-520.
61.Bianco P,Riminucci M,Gronthos S,et al.Bone marrow stromal stem cells:nature,biology,and potential applications[J]..Stem Cells,2001,19(3):180-192.
62.Martinez C,Hofmann TJ,Marino R,et al.Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2:a novel surface marker for the identification of MSCs[J].Blood,2007,30:336-338.
63.DBaksh L.Song RS.Tuan.Adult mesenchymal stem cells:characterization,differentiation,and application in cell and gene therapy[J].Cell Mol,2004,8(3):301-316.
64.连霞,李光来.骨髓间充质干细胞生物学特性及其多向分化潜能的理论基础和机制[J].临床医药实践杂志,2006,15(3):163-165.
65.林建华,雷盛民,康德智,等.静脉注骨髓间质干细胞对脊髓损伤修复作用的实脸研究[J].中华骨科杂志,2005,25(9):556-558.
66.Vulliet PR,Greeley M,Halloran SM,et al.Intra-coronary arterial injection of mesenchymal stromal cells and microinfarction in dogs[J].Lancet,2004,363(9411):783-784.
67.Lu L,Zhao C,Liu Y,et al.Therapoutic benefit of TH-engineered mesenchymal stem cells for Parkinson's disease[J].BrainRes Brain Res Protocol,2005,15(1):46-51.
68.Mimura T,Dezawa M,Kanno H,et al.Peripheral nerve regeneration by transplantation of bone marrow stromal cell-derived Schwann cells in adult rats[J].J Neuro surg,2004,101(5):806-812.
69.SAKAI D,MOCHIDA J,YAMAMOTO Y,et al.Tranlantation of mesenchymal stem cells embedded in Aieloeollagen gel tothe inter-vertebral disc:a potential therapeutic model for disc degeneration [J].Biomaterials,2003,24(20):3531-3541.
70.Woo SL,Hildebrand K,Watanabe N,et al.Tissue engineering of ligament and tendon healing[J].Clin Orthop.2006,12(11):312-323.
71.Jorgensen C,Djouad F,Apparailly F,et al.Engineedng mesenchymal stem ceils or immuao-therapy[J].Gene Ther,2003,23(10):928-931.
72.Woodbury D,Schwarz EJ,Prockop DJ,et al.Adult rat and human bone marrow stromal cells differentiate into neurons[J].J Neurosci Res,2000,61(4):364-370.
73.Deng W,Obrocka M,Fischer I,et al.In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intraccllular cyclic AMP[J].Biochcm Biophys Res Commun,2001,282(1):148-152.
74.项鹏,夏文杰,张丽蓉,等.成人骨髓间质干细胞定向诱导为神经元样细胞的研究[J].中国病理生理杂志,2001,17(5):385-387.
75.张卉,王纪佐,孙红宇,等.成鼠骨髓基质细胞分化成神经元的体外研究[J].脑与神经疾病杂志,2001,9(6):336-338.
76.李德华,单伟,张海锋.体外诱导人脐静脉间充质干细胞分化为神经元样细胞的实验研究[J].神经解剖学杂志,2007,23(5):554-558.
77.辛颖,李玉林,张丽红.成人骨髓间充质干细胞体外定向诱导分化为神经元样细胞的研究[J].中国实验诊断学2007,11(1):4-6.
78.施雪英,陈先文,任于果.大鼠骨髓间充质干细胞在体外向神经元样细胞的分化[J].安徽医科大学学报,2006,41(1):16-19.
79.Black I,Woodbury D..Adult rat and human bone marrow strom stem cells differentiate into neurons[J].Blood Cells MolDis,2001,27:632-636.
80.Kohyama J,Abe H,Shimazaki T,et al.Brain from bone:efficient "meta differentiation"of marrow stromaderived mature osteoblasts to neurons with noggin or ademethylating agent[J].Differentiation,2001,68:235-244.
81.Sanchez-Rarnos J,Song S,Cardozo-Pelaez F,et al,Adult bone marrow stromal cells diferentiate into neural cells in vitro[J].Exp Neurol,2000;164(2):247-256.
82.徐如祥,戴宜武,姜晓丹,等.成年骨髓间质干细胞体外诱导分化成神经细胞研究[J].中华神经外科疾病研究杂志,2002,1f1):63-67.
83.许期年,金钧,苗宗宁.脑源性神经生长因子促进骨髓间充质干细胞分化为神经元样细胞的实验研究[J].苏州大学学报(医学版)2007,27(4):520-522.
84.梅晰凡,王伟,刘品端.碱性成纤维细胞生长因子和神经生长因子联合诱导成年鼠骨髓基质细胞向神经元样细胞的分化[J].中国临床康复,2006,10(13):32-35.
85.叶民,陈生弟,陆国强,等.成年大鼠骨髓基质干细胞诱导分化为神经元样细胞不同方法比较的研究.[J]中国神经科学杂志.2004,20(4):275-281.
86.钟春玖,王志军,金莉蓉.诱导骨髓基质细胞表达神经细胞标记物Nestia,NSE和GFAP:脑源性神经节苷脂定向诱导细胞分化作用的研究[J].中国临床医学,2005,3(5):761-762.
87.Quirici N,Soligo D,Bossolasco P,et al.Isolation of bone marrow mesenchymal stem cells by anti—nerve growth factor receptor antibodies[J].Exp Hematol,2002;30(7):783-791.
88.Satomura K,Derubeis A,Fedarko NS,et ol.Receptor tyrosine kinase expression in human bone marrow stromal cells[J].Cell Physical,1998:177(3):426-438.
89.Tokumine J,Kakinohana O,Cizkova D,et ol.Changes inspinal GDNF,BDNF and expression after transient spinal cord ischemia in the mice[J].Neurosci Res,2003:74(4):552-561.
90.Tassi E,AlAttar A,Aigner A,et ol.Enhancement of fibroblast growth factor(FGF)activity by an FGF—binding protein[J].Biol Chem,2001;276(43):40247-40253.
91.黄慧,唐云安,张成.香丹注射液定向诱导大鼠骨髓间质干细胞分化为神经元样细胞[J].中药材,2004,27(8):585-589.
92.刘金保,董晓先,董燕湘等.当归诱导骨髓间充质干细胞分化为神经元样细胞[J].广东医学,2003,24(5):466-468.
93.赵楠,冯忠堂,王廷华等.丹参注射液诱导骨髓基质细胞分化为神经元的研究[J].昆明医学院学报,2004,25(4):27-29.
94.黄洁,张宜,杜少辉等.牛珀至宝微丸对骨髓间充质干细胞移植后转分化为神经元的影响.[J]中华中医药杂志,2005,20(12):721-723.
95.董晓先,刘金保,董燕湘等.天麻诱导骨髓间质干细胞分化为神经元细胞的实验研究[J].中国中西医结合杂志,2004,24(1):51-54.
96.张进,徐志伟.补肾法诱导间充质干细胞向神经方向分化研究[J].现代医院,2004,4(9):15-17.
97.王勇,陆长青,王凡.黄芪诱导大鼠骨髓间充质干细胞分化为神经样细胞的研究[J].四川解剖学杂志,2006,14(1):5-8.
98.陈东风,杜少辉,李伊为等.龟板含药血清体外诱导成年大鼠骨髓间充质干细胞分化为神经元[J].广州中医药大学学报,2003,20(3):224-226.
99.陆长青,王勇,陈登榜等.丹参诱导骨髓基质干细胞向神经元样细胞分化及相关基因的表达[J].四川解剖学杂志,2006,14,(1):1-4.
100.董燕湘,董晓先,何慧华等.大鼠骨髓间质干细胞用中药绞股蓝诱导为神经细胞的研究[J].中华神经科杂志,2003,36(5):355-358.
101.撒亚莲,李海标.川芎嗪诱导大鼠骨髓间质干细胞分化为神经元样细胞的研究[J].解剖学报,2003,34(5):515-518.
102.余勤,罗依,鄂艳等.丹参素定向诱导骨髓间质干细胞分化为神经元样细胞的研究[J].中国中西医结合杂志,2005,25(1):49-54.
103.蔡光先,林琳刘柏炎等.地黄多糖诱导骨髓间充质干细胞分化为神经样细胞的效应[J].中国临床康复,2005,9(17):17-19.
104.项平,李海标.黄连素诱导大鼠骨髓间质干细胞分化为神经元样细胞[J].中国病理生理杂志,2004,20(1):51-53.
105.贾延劼,杨于嘉,周燕等.黄芩甙体外诱导大鼠骨髓基质细胞成为神经细胞[J].中华医学杂志,2002,82(19):1337-1342.
106.唐云安,王瑞淑,张成.大鼠脊髓匀浆上清液对骨髓间质干细胞的诱导分化作用[J].中风与神经疾病杂志,2003,20(3):196-199.
107.宋永周,崔慧先,吕哲,等.创伤性脑组织匀浆对大鼠骨髓间充质干细胞分化为神经元样细胞的影响[J].中国组织工程研究与临床康复,2008,12(3):461-463.
108.牛朝诗,孔杰.损伤脑组织匀浆诱导骨髓间质干细胞向神经细胞分化的研究[J].立体定向和功能神经外科杂志,2006,19(5):270-272.
109.张燕青,曾园山,张静.体外培养的嗅鞘细胞能够促进骨髓间充质干细胞分化为神经元样细胞[J].中国神经精神病杂志,2007,33(10):611-614.
110.牛云,何旭,王心蕊.鼠胶质瘤细胞上清液诱导人骨髓间充质干细胞向神经元样细胞的分化[J].吉林大学学报(医学版),2007,33(4):635-637.
111.陈宁,李昌琪,罗学港.骨髓基质干细胞诱导转化与移植[J].国外医学·生理、病理科学与临床分册,2002,23(5):534-537.
112.闰彬彬,王莉莉,乔俊峰等.体外诱导骨髓基质干细胞向神经元分化的机制研究[J].细胞与分子免疫学杂志[J],2005,21(3):301-304.
113.Denrus J E,Charbord P.Origin and differentiation of Human and Murine Stroma[J].Stem cells,2002,20(3):205-214.
114.高健,刘振华,姜晓丹等.骨髓源性神经干细胞诱导分化为多巴胺能神经元的实验研究.中华神经医学杂志[J],2006,5(4):349-352.
115.Yamaguchi A,Komori T,Suda T.Regulation of osteoblast differentiation mediated by bone morphogenetic proteins,hedgehogs,and Cbfal[J].Endocrine Rev,2000,21(4):393-411.
116.Deng W,Obrocka M,Fischer I,et al.In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracell ularcyclic AMP[J].Biochem Bioph Res Co,2001,282(1):148-152.
117.王海燕,徐如祥,罗深秋等.多细胞因子诱导成年鼠骨髓基质细胞向神经细胞分化的实验研究[J].中国微侵袭神经外科杂志,2004,9(2):77-81.
118.Sanchez-Ramos J,Song S,Cardozo-Pelaez F.et al.Adult bone marrow stromal cells differentiate into neural cells in vitro[J].Exp Neurology,2000,164(2):247-256.
119.Woodbery D,Reynolds K,Black I B.Adult bone marrow stromal stem cells express germline,ectodermal,endodermal,and mesdermal genes prior to neurogenesis[J].Neurosci Res,2002,69(6):908-917.
120.Weslet-Gendebien S,Hans G,Leprince P,et al.Plasticity of cultured mesenchymal stem cells:switch from nestin-positive to excitable neuron-like phenotype[J].StemCells,2005,23(3):392-402.
121.Jori FP,Melone MA,Napolitano MA,et al.RB and RB2/PBO genes demonstrate both specific and overlapping functions during the early step of in vitro neural differentiation of marrow stromal stem cells[J].Cell Death Differ,2005,12(1):65-77.
122.Jori FP,Napolitano MA,Melone MA,et al.Molecular pathways involved in neural in vitro differentiation of marrow stromal stem cells[J].J Cell Biochem,2005,94(4):645-655.
123.冯英,谢畜康等.骨髓基质干细胞诱导成神经元样细胞分化相关基因的表达[J].中国病理生理杂志,2004,20(12):2268-2271.
124.Shafit-Zagardo B,Kalcheva N.Making sense of the multiple MAP-2 transcripts and their role in the neuron[J].Mol Neurobiol,2001,16(2):149-162.
125.Kaech S,Parmar H,Roelandse M,et al.Cytoskeletal microdifferentiation:a mechanism for organizing morphological plasticity in dendrites[J].Proc Natl Acad Sci USA,2003,98(13):7086-7092.
126.任宁,王新平,张文治.骨髓间充质干细胞向神经细胞分化的研究进展[J].国际脑血管杂志,2006,14(1):65-68.
127.高晓山.病理生理条件下中药十八反实验研究的综合报告[J].中医杂志,1991;(1):36-38.
128.关天增,潘先琼,常洪志.等.“诸参辛芍叛藜芦”之实验研究[J].河南中医,1993;13(5):204-205.
129.金恩波,姜名瑛,黄启福,等.中药十八反的药理研究[J].中成药研究,1982;(2):28-31.
130.《中华人民共和国药典》[M].国家药典委员会编.北京:化学工业出版社,2005:199.
131.李同琴,郭秋红,仝利琪.党参反藜芦的动物实验研究.陕西中医,2003,23(8):744.
132.杨胜辉,刘威.十八反中几组药物配伍的临床应用.陕西中医,2000,21(4):179.
133.《中华人民共和国药典》.国家药典委员会编.北京:化学工业出版社,2005:52-53.
134.LAYI S,HSIEH CC,CHIUJ H,et al.Salvianolic acid B enhances in vitro angiogenesis and imp roves skin flap survival in Sprague-Dawley rats[J].Surg Res,2003,115(2):279-286.
135.GES R,YUY X,XIEG X.Advances in studies on pharmacological activities of salvianolic acids[J].Chin M ed Material,2002,25(9):683-685.
136.L IANG Y,YANG YM,YUANS L.Advances in studies on pharmacological activities and clinical applications of Tanshinones[J].Chin Tradit Herb D rugs,2000,31(4):304-306.
137.赵娜,郭治昕,赵雪.丹参的化学成分与药理作用[J].国外医药·植物药分册,2007,22(4):155-157.
138.杜冠华,张均田.丹参现代研究概况与进展[J].医药导报,2004,23(6):355-361.
139.叶勇.丹参有效成分分离的研究进展[J].药品评价,2005,2(2):146-148.
140.祖淑玉,高咏梅,朱广瑾.丹参对血管紧张素诱导内皮细胞组织因子基因的表达的作用[J].中华老年心血管病杂志,2004,6(4):257-260
141.Ding M,Zhao G R,Ye T X,et al.Salvia miltiorrhiza protect sendothelial ceils against oxidative stress[J].Altem Complement Med,2006,12(1):5-6.
142.Ling S H,Dai A Z,Guo Z X,et al.Effects of a Chinese herbal preparation on vascular cells in culture:mechanisms of cardiovascular protection[J].Clin Exp Pharmacol Physiol,2005,32(7):571-578.
143.孙可青,徐长庆.丹参素的抗心律失常作用及其电生理机制的研究[J].中国中医药科技,2000,7(3):171-172
144.徐长庆,王孝铭,范劲松,等.丹参酮Ⅱ-A对豚鼠单个心室肌细胞跨膜电位及L-型钙电流的影响[J].中国病理生理杂志,1997,13(1):43-47.
145.Chang P N,Mao J C,Huang S H,et al.Analysis of cardioprotective effects using purifiedSalvia miltiorrhiza extract on isolated rat hearts[J].Pharrnacol Sci,2006,101(3):245-249
146.史举彤,杨文浩,陈长清.丹参素对体外循环中血细胞的影响[J].山东医科大学学报,1991,29(3):237-239.
147.Imanshahidi M,Hosseinzadeh H.The pharmacological effects of Salviaspecies on the central nervous system.[J].Phytother Res,2006,20(6):427-437
148.刘军,匡培根,吴卫平,等.大鼠脑缺血再灌注区小胶质细胞反应及丹参的影响[J].中华老年心脑血管病杂志,2002,4(2):127-129
149.沈霞,崔桂云,张璐,等.丹参对大鼠全脑缺血再灌注后海马CA1区AP1DNA结合活性的影响[J].江苏医药杂志,2004,30(6):422-424.
150.Lao C J,Lin J G,Kuo J S,et al.Effect ofSalvia miltiorrhiza bunge on cerebral infarct in ischemia-reperfusion injured rats[J].Am J Chin Med,2003,31(2):191-200.
151.董静,罗桂林,苗维纳,等.丹参对实验性肺纤维化小鼠病理变化和核因子κB表达的影响[J].中国药理学通报,2003,19(12):1428-1431.
152.姜慧卿,王占魁,张晓岚,等.丹参单体IH764-3抗胆总管结扎大鼠胶原降解作用及其机制[J].胃肠病和肝病学杂志,2003,12(4):336-338.
153.吴杲,何招兵,吴汉斌.丹参酮的药理作用研究进展[J].现代中西医结合杂志,2005,14(10):1382-1385.
154.Soung do Y,Rhee S H,Kim J S,et al.Peroxynitrite scavenging activity of lithospermate B fromSalvia miltiorrhiza[J]..Pharm Pharmacol,2003,55(10):1427-1432
155.Ding M,Ye T X,Zhao G R,et al.Aqueous extract of Salvia miltiorrhizaattenuats increased endothelial permeability induced by tumor necrosis factor-α.[J].Int Immuno pharm,,2005,5(11):1641-1651.
156.Kwon H J,Hyun S H,Choung S Y.Traditional Chinese medicine improves dysfunction of peroxisome proliferators activated receptor alpha and mierosomal triglyeeride transferprotein on abnormalities in lipid metabolism in ethanolfed rats[J].Biofactors,2005,23(3):163-176.
157.汤建,李慧梁,黄海强.藜芦属植物化学成分的研究近况[J].药学进展,2006.30(5):206-209.
158.赵录英,赵画晨,吴博威.藜芦混碱对离体心泵功能的效应及其与洋地黄类药物的对比[J].山西医科大学学报,2002,33(3):231-232.
159.康毅,王国祥.牛磺酸抗藜芦碱实验性心律失常作用[J].天津医药,1989,17(4):199-201.
160.中国药科大学、中国医药出版社.中药辞海(第四卷)[M].北京:中国医药科技出版社,1998:8-13.
161.李淑媛,李卫平,李传勋.盐酸乌苏里藜芦碱对麻醉犬血流动力学的研究[J].中国药学杂志,1997,32(7):407-409.
162.刘燕,高广猷,叶丽虹.光脉黎芦碱的降压作用[J].大连医学院学报,1991,13(4):68-71.
163.李华,高广猷,李淑媛,等.乌苏里藜芦碱对肾性高血压大鼠中枢儿茶酚胺能神经元的作用[J].中国药理学报,2000,21(1):23-28.
164.马丽焱,周远鹏,江京俐.狭叶藜芦碱甲对犬血流动力学的影响[J].中草药,1998,29(2):105-107.
165.李欣燕,任洪灿,韩国柱.乌苏里藜芦碱在大鼠的血液流变学作用[J].大连医科大学学报,2004,26(3):165-167.
166.李欣燕,韩国柱,张书文.乌苏里藜芦碱对血小板聚集及凝血与出血时间的影响[J].中草药,2004,35(11):1269-1272.
167.段建红,段玉斌,邢俊玲,等.藜芦碱致使大鼠背根神经节A类神经元产生触发性振荡[J].生理学报,2002,54(3):208-212.
168.雷革胜,朱俊玲,万业宏,等.小剂量藜芦碱诱发大鼠脑海马CA1区锥体神经元异常放电癫痫脑片模型的特征[J].中国临床康复,2005,9(25):238-239。
169.由广旭,周琴,李卫平.乌苏里藜芦碱对大鼠脑缺血-再灌注损伤的保护作用[J].中草药,2004,35(8):908-911.
170.雷革胜,朱俊玲,万业宏,等.小剂量藜芦碱诱发大脑海马CA1区锥体神经元异常放电癫痫脑片模型的特征[J].中国临床康复,2005,9(25):238-239.
171.郑善子,崔春权,刘永镇.藜芦乳膏对毛囊蠕形螨的体外杀虫作用[J].延边大学医学学报,2004,27(1):31-32.
172.杨素华,李东宁,孙健.藜芦乳膏治疗疥疮的疗效观察[J].辽宁中医杂志,2002,29(11):664.
173.李太一,申成华,郑善子,等.藜芦乳膏的皮肤毒性实验研究[J].延边大学医学学报,2004,27(2):118-120.
174.徐国钧.徐珞珊,王峥涛.常用中药材品种整理和质量研究(第四册)[M].福建:福建科学技术出版社,2001:121-178.
175.毛晓峰,史志诚,王亚洲.我国藜芦属植物研究进展[J].动物毒物学,2003,18(1-2):17-21.
176.王延章.重审十八反[M].北京:中国中医药出版社,1992:23-59.
177.陈馥馨.782个含十八反、十九畏内服成药方组成与主治分析[J].中国医药学报,1987,(2):26.
178.楼朱雄,杨建国,王健生,等.HPLC法测定藜芦配丹参后丹参酮ⅡA含量的变化[J].中药材,1993,16(10):32.
179.王宇光,高月,柴彪新,等.人参、藜芦合用对大鼠肝P450酶活性及mRNA表达的调控作用[J].中国中药杂志,2004,29(4):366-370.
180.沈光稳·采用发光细菌研究探讨“十八反”配伍药物的临床毒性[J].中医药研究,1998,14(1):10.
181.Chem V,Yip B,Yam I,et al.Carrier incidence for spinal muscul aratrophy in Southern Chinese[J].Neurol,2004,251(9):1089-1093.
182.Yip B,Yam I,杜芳,et a.l.中国南方脊髓性肌萎缩的基因携带率[J].世界核心医学期刊文摘.神经病学分 册,2005,(2):26-27.
183.倪昊,王立文.脊髓性肌萎缩症及临床相关SMN基因的研究进展[J].国际儿科学杂志,2006,33(1):26-29.
184.Lefebvre S,Burlet P,Liu Q,et al.Correlation between severity and SMN Protein level in spinal muscul aratrophy[J].Nat Genet,1997,16(3):265-269.
185.Coovert DD,Le TT,McAndrew PE,et,al.The survival motor neuron protein in spinal muscular atrophy [J].Hum Mol Genet,1997,6(8):1205-1214.
186.Carter TA,Bonnermann CG,Wang CH,et al.A multicopy transcrip tion-repair gene,BTF P44,maps to the region and demonstrates SMA associated deletions[J].Hum Mol Genet,1997,6:229-236.
187.Scharf JM,Endrizzi MG,Wetter A,Huang S,et al.Identification of a candidate modifying gene for spinal muscular atrophy by comparative genomics[J].Nature Genet,2005,20:83-86.
188.Lcfebvre S,burglen L,Frezal J,et al.The role of SMN gene in proximal spinal muscular atrophy[J].HumMol Genet,1998,7:1531-1536.
189.邢晓为,傅俊江.脊髓性肌萎缩症的分子遗传学研究新进展[J].国外医学·生理、病理科学与临床分册,2001,21(6):438-450.
190.Monani UR,Lorson CL,Parsons DW,et al.A single nueleotide difference that alters splicing patterns distinguishes the SMA gene SMN1 from the copy gene SMN2[J].HumMol Genet,2003,8:1177-1183.
191.Lorson CL,Halmen E,Androphy EJ,Wirth B,et al.A single nueleotide in the SMN gene regulates splicing and is responsible for spinal muscular atrophy[J].Proc Natl Aead Sei USA,1999,96(11):6307-6311.
192.Coovert DD,Le TT,McAndrew PE,et,al.The survival motor neuron protein in spinal muscular atrophy[J].Hum Mol Genet,2006,6(8):1205-1214.
193.Lefebvre S,burglen L,Reboullet S,et al.Identification and characterization of spinal muscular atrophydetermining gene[J].Cell,1995,,80:167.
194.杨晓苏,张丽芳,肖波.SMN蛋白与脊髓性肌萎缩症的研究进展[J].中国神经科学杂志,2002,18(4):740-742.
195.Pellizzoni L,Charroux B,Dreyfuss G.SMN mutants of spinal muscular atrophy patients are defective in binding to snKNP proteins[J].Proc Natl Acad Sci USA,2003,96(20):11167-11172.
196.Li HMH,Chang JG,et al.The effect of inactivation of SMN gene on mouse[J].Nat Genet,2003,24:66-70.
197.Wirth B.An update of the mutation spectrum of the survival motor neuron gone(SMN1) in autosomal recessive spinal muscular atrophy(SMA)[J].Human Mutation,2001,15:228-237.
198.Wirth B,Herz W,Wetter A,Moskau S,et al.Quantitative analysis of survivalmotor neuron copies:identification of subtle SMN1 mutations in patientswith sp inal muscular atrophy,genotype-phenotype correlation,and imp lieation for genetic counseling[J].Am J Hum Gcnet,1999,64:1340-1356.
199.Feldkotter M,Schwarzer V,Wirth R,et al.Quantitative analyses of SMN1 and SMN2 based on real-time LightCyeler PCR:fast and highly reliable carrier testing and p rcdiction of severity of sp inalmnscular atrophy[J].Am J Hum Genct,2002,70:358-368.
200.Wirth B,Sehmidt T,P,,udnik-Sehoneborn S,et al.De novo rearrangements found in 2%index patientswith spinal muscular atrophy(SMA):mutation mechanisms,parental origin,mutation rate and implications for prenatal diagnosis[J].Cell,2004.,56:573-576.
201.Rossoll W,Jablonka S,Andreassi C,et al.Stun,the spinal muscular atrophy Determing gene product,modulates axon growth and localization of beta-actin mRNA in growth cones of moto-neurons[j].Cell Biol,2003,163(4):801-812.
202.Coovert DD,Le TT,McAndrew.PE,et al.The survival motorneuron protein in spinal muscular atrophy [J]..Mol Genet,1997,6:1205-1214.
203.倪昊,王立文.脊髓性肌萎缩症及临床相关SMN基因的研究进展[J]..国际儿科学杂志,2006,33(1):26-28.
204.Rossoll W,Jablonka S,Andreassi C,et al.Smn,the spinal muscular atrophy determing gene product,modulates axon growth and localization of beta2actinmRNA in growth cones of motoneurous[J].Cell Biol,2003,163(4):801-812.
205.Meister G,Buhler D,Laggerbauer B,et al.Characterization of a nuclear 20S complex containing the survival of motor neurons(SMN) protein and a specific subset of spliceosomal Sm proteins[J]...Hum Mol Genet,2000,9:1977-1986.
206.Campbell L,Hunter KM,Mohaghegh P,et al.Direct interaction of SMN with dp103,a putative RNA helicase:a role for SMN in transcription regulation[J].Hum Mol Genet,2000,9:1093-1100
207.Liu Q,Fischer U,Wang F,Dreyfuss G.The sp inalmuscular atrophy disease gene product,SMN and its associated protein SIPI are in complex with splice osomal snRNP proteins[J].Cell,2004,90:1013-1017.
208.Pagliardini S,Giavazzi A,Setola V,et al.Subcellular localization and axonal transport of the survival motor neuron(SMN) protein in the developing rat spinal cord[J]..Hum Mol Genet,2000,9:47-56.
209.Jones MA,McEwen IR,Hansen L.Use of power mobility for a young child with spinal muscular atrophy[J].Phys Ther,2003,83(3):253-262.
210.Cusco I,Barcelo MJ,Soler C,et al.prenatal diagnosis for risk of spinal muscular atrophy[J].BJOG,2002,109(11):1244-1249.
211.Migita M,Migita M,Uchikoba Y,et al.Genetic diagnosis of Werdnig Hoffmann disease:a problem for application to prenatal diagnosis[J].Nippon Med Sch,2003,70(1):45-48
212.Moutou C,Gardes N,Viville S.Duplex PCR for preimplantation genetic(PGD) of spinal muscular atrophy[J].prenat Diagn,2003,23(8):685-689.
213.Bergeijk J,Haastert K,Grothe C,et al.Valproic acid promotes neurite outgrowth in PC12 cells independent from regulation of the survival of motor neuron Protein.[J].Chem Biol Drug Des,2006,67:244-24.
214.Andreassi C,Jarecki J,Zhou J,et al.Aclarubicin treatment restores SMN levels to cells derived from type I spinal muscular atrophy patients[J].Hum Mol Genet,2001,10(24):2841-2849.
215.倪昊,王立文.脊髓性肌萎缩症及临床相关SMN基因的研究进展[J].国际儿科学杂志,2006,33(1):26-28.
216.Rossoll W,Jablonka S,Andreassi C,et al.Smn,the spinal muscular atrophy determing gene product,modulates axon growth and localization of beta2actinmRNA in growth cones of motoneurons[J].JCell Biol,2003,163(4):801-812.
217.Zhiming Yang,Yu-Jia Chang,I-Chen Yu,et al.ASC-J9 ameliorates spinal and bulbar muscular atrophy phenotype via degradation of androgen receptor[J].Nature Medicine,2007,13:348-353.
218.杨晓苏,吴海香,肖波.人骨髓间质干细胞分化为神经元样细胞及SMN蛋白表达的研究[J].中华医学杂志,2005,85:1121-1128.
219.罗新明,杨晓苏,肖波.脊髓性肌萎缩症患者神经元样细胞SMN2基因mRNA的表达[J].中华内科杂志,2006,45(10);831-833.
220.丁伟荣,吴晓牧,饶燕飞,等.人骨髓间充质干细胞体外分离培养方法的比较研究[J].江西医药,2007,42(2):115-117.
221.Jiang Y,Jahagirdar BN,Reinhardt RL,et al.Pluripotency of mesenchymal stem cells derived from adult marrow[J].Nature,2002,41841-49.
222.Friedenstein AJ,Piatetzky Shapiro Ⅱ,Petrakova KV.Osteogenesis in transplants of bone marrow cells.[J].Embryol Exp Morphol,1966,16(3):381.
223.Kuo CK,Tuan RS.Tissue engineering with mensenchymal stem cells[J].IEEE Eng Med Bio Mag,2003,22(5):51
224.Shin M,Yoshimoto H,Vacanti JP.In vivo bone tissue engineering using mesenchymal stem cells on a novel electrospun nano fibrous scaffold[J].Tissue Eng,2004,10(1-2):33
225.Pittenger MF,Mackay AM,Beck SC,et al.Multilineage potential of adult human mesenchymal stem cells[J].Science,1999,284(5411):143.
226.徐忠华,吴清玉.贴壁法分离培养大鼠骨髓间充质干细胞的生物学特性[J].中国组织化学与细胞化学杂志,2007,16(5):554-556.
227.代飞,吴军,许建中,等.两种从骨髓中分离人间充质干细胞方法的比较研究[J].第三军医大学学报,2005,27(16):1631
228.Coher DC,Clas R,DiGirolanao CM,et al.Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow[J].Proc Natl Acad Sci USA.2000,97(7):3213-3218.
229.陈欢意,牛平,赵帅,杜迎春.改良贴壁培养法简单的骨髓基质干细胞分离纯化法[J].解剖科学进展,2007,13(2):190-192.
230.Colter DC,Sekiya I,Prockop DJ.Identification of a subpopulation of rapidly self-renewing and mul-tipotential adult stem cells in colonies of human marrow stromal cells[J].Proc Natl Acad Sci USA.2001,98:7841-7845.
231.杨丽.OP模鼠MSCs生物学特性及药物对大鼠MSCs骨向分化的干预研究.暨南大学2004级博士学位论文,37.
232.Majumdar MK,Keane-Moore M,Buyaner D,et al.Characterization and functionality of cell surface molecules on human mesenehymal stem cells[J].Biomed Sci,2003;10(2):228-241.
233.Tropel P,Noel D,Platet N,et al.Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow[J].Exp Cell Res,2004,295(2):395-406.
234.HOR I Y,NAKAMURA T,KIMURA D,et al.Experimental study on tissue engineering of the small intestine bymesenchymal stem cell seeding[J].Surg Res,2002,102(2):156-160.
235.Devine SM.Mesenchymal stem cells:will they have a role in the clinic?[J].J Cell Biochem,2002;38[Suppl]:73-79.
236.Pittenger MF,Mackay AM,Jaiswal SC,et al.Multilineage potential of adult human mesenchymal stem cells[J].Science,1999;254(541):143-147.
237.杨丽.OP模鼠MSCs生物学特性及药物对大鼠MSCs骨向分化的干预研究.暨南大学2007年博士学位论文,101-103.
238.关宁,罗俊生,刘兴波,等.β-巯基乙醇和神经生长因子定向诱导鼠骨髓基质细胞分化为神经细胞的验证[J].中国组织工程研究与临床康复,2007,11(3):451-453.
239.龚理.NG对OP大鼠MSCs增殖、骨向分化及凋亡影响的实验研究.暨南大学2006年硕士学位论文,45-47.
240.Krause DS.Plasticity of marrow-derived stem cells[J].Gene Therapy,2002:9:754-758.
241.Erica L,Herzog,LiChai,et al.Plasticity of majrrow derived stem cells[J].Blood,2003,102(10):3483-3493.
242.李健,牛朝诗,傅先明.bFGF和N2体外联合定向诱导骨髓间质干细胞向神经元样细胞分化[J].立体定向和功能性神经外科杂志,2004,17(3):144-147.
243.陈宁,李昌琪,罗学港.骨髓基质干细胞诱导转化与移植[J].国外医学·生理、病理科学与临床分册,2002,23(5):534-537.
244.闰彬彬,王莉莉,乔俊峰等.体外诱导骨髓基质干细胞向神经元分化的机制研究[J].细胞与分子免疫学杂志,2005,21(3):301-304.
245.冯英,谢富康等.骨髓基质干细胞诱导成神经元样细胞分化相关基因的表达[J].中国病理生理杂志,2004,20(12):2268-2271.
246.张蒙夏,谢富康.成年SD鼠骨髓基质干细胞向神经元样细胞分化过程中分化与凋亡关系的研究[J].中国病理生理杂志,2003,19(1):32-35.
247.夏文杰,陈振光,张丽蓉等.隐丹参酮诱导骨髓间充质干细胞分化为神经元样细胞的实验研究[J]。中国中西医结合杂志,2002,22(12):921-924.
248.Wang LF,Zhao～HY.A theoretical investigation on DPPH radiealscavenging mechanism ofaravone[J].Bioorg Med Chem Lett,2003,13(21):3789-3792.
249.Takeda Y,Sato H,Satoh M,etal.Immunohistochemical expression of neural tissue markers(neuron-specific enolase,glial fibrillary acidic protein,S100 protein) in ameloblastic fibrodentinoma:a comparative study with ameloblastic fibroma[J].Pathol Int.2000,50(8):610-615.
250.Pekny M,Johnasson CB,Eliasson C,et al.Abnomral reation to eenrtal nervous system injury in mice lacking glial fibrillayr aeidic protein and vimentin[J].Cell Biol,1999,145(3):503-514.
251.Maslov AY,Barone TA Plunker RJ,et al.Neuarl stem cell detection,characterization,and age-related changes in the subvertrtieular zone of mice[J].Neuorsci,2004,24(7):1726-1733.
252.Kobayashi M,Sjobegr G,Sodehtall S,et al.Pediartic rhabdomyosacromas express the internrediate filament nestin[J].pediart Res,2005,43(3):386-392
253.KemPernrann G,Kronenberg G,et al.Early determination and long tens persistence of dault generated new neurons in hippoeampus mice[J].Development,2003,130(2):391-399.
254,Sanehez-Ramos JR,Cardozo-Pelaez F,Song S.Differentiation of neuron-like cells from bone marrow stromal cells[J].Mov Disord,1998,13(Suppl):122.
255.Woodury D,Schwarz EJ,Prockop DJ,et a.l Adult rat and human bone marrow stromal cells differentiate into neurons[J].Neurosei Res,2000,61(4):364-370.
256.Mezey Eva,Keys,VogelsangG,et a.l Transplanted bone marrow generates new neurons in human brains [J].PNAs,2003,100(3):1364-1369.
257.余勤,罗依,鄂艳,等.丹参素定向诱导骨髓间质干细胞分化为神经元样细胞的研究[J].中国中西医结合杂志,2005,25(1):49-54.
258.施雪英,陈先文,任于果,等.大鼠骨髓间充质干细胞在体外向神经元样细胞的分化[J].安徽医科大学学报,2006,41(1):16-18.
259.Ong SY,Dai H,Leong KW.Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture[J].Biomaterials 2006;27(22):4087-4097.
260.关宁,罗俊生,刘兴波,等.β-巯基乙醇和神经生长因子定向诱导鼠骨髓基质细胞分化为神经细胞的验证[J].中国组织工程研究与临床康复2007,11(3)2:451-454.
261.Majumdar MK,Thiede MA,Mosea JD,etal.Phenotypic and functional comparison of marrow derived mesenehymal stem cells[J].Cell physiol,2005,176(1):56-59.
262.Weiss S,Jehon Y.Pathways for neural stem cell biology and repair[J].Nat Bioteehnol,2004,17(9):850-851.
263.Sanehez Rarnos J.Adult bone marrow stromal cells differentiate into neural cells in vitro[J].Exp Neurol,2000,1 64:247-256.
264.Wurmser AE,Cage FH.Stem cell:Cell fusion causes confusion.[J]Nature,2002,416(6880):485-487.
265.Rietze RL,Valeanis H,Brooker CF,et al,Purification of a pluripotent neural stem cell from the adult mouse brain[J].Nature,2001,412(6848):736-739.
266.姚晓黎,张成,冯善伟,等.人骨髓间充质干细胞分化为神经元样细胞的逆转化现象[J].第一军医大学学报,2005,25(50:513-515.
267.DengW,Obroeka M,Fisher 1,et al.Adult bone marrow stromal cells differentiate into neural cells in vitro[J].Biochem Biophys Res Commun,2001,282:148-152.
268.Woodbury D,Reynolds K,Black IB.Adult bone marrow stromal stem cells express germline,ectodermal,endodermal,and mesodermal genes prior to neurogenesis[J].Neurosei Res,2002,96:908-917.
269.江和碧,卓本慧,代英等.bFGF促进大鼠骨髓间充质干细胞向神经元转化及机制[J].第三军医大学学报,2005,27(2):98-101.
270.Prockop DJ..Marrow stromal cell as stem cells for nonhematopoietis tissues[J].Science,1997,276(5309);71-74.
271.Vander Steege G,Draaijer TG,Groots cholten PM,et al.PCR-base DNA test to confirm the clinical diagnosis of autosomal recessive spinalmuscular atrophy.[J]Lancet,1995,345:985-986.
272.杨晓苏,张丽芳,肖波.SMN蛋白与脊髓性肌萎缩症的研究进展[J].中国神经科学杂志,2002,18(4):740-743.
273.Manzur A,Muntoni F,Simonds A.Muscular Dystrophy Campaign sponsord workshop:Recommendation for Respiratory Care of Children with Spinal Muscular Atrophy Type Ⅱ and Ⅲ[J].Neuro muscul Disord,2003,13(2):184-189.
274.JonesMA,McEwen IR,Hansen L.Use of power mobility for a young child with spinal muscular atrophy[J].PhysTher,2003,83(3):253-262.
275.Richardson RM,F illmoer HL,H olloway KL,et al.Progress in cerebraltran splantation of expanded neuronal stem cells[J].Neurosurg.,2004.100(4):659-671.
276.Eriksson C,B jorklund A,W ictorin K.Neuronal diferentiation following transplantation of e xpanded mouse neuro sphere cultures derived from different embry of brain regions[J].Exp Nenrol.2003 184(2):615-35.
277.Alvarez-BuyllaA,L im DA,et al.For the long run:maintaining germinal niches in the adult brain.[J]Nenron.2004.41(5):683-685.
278.Liang P,ZhaoS,K awamotoK,et al.Neuronal and glial diferentiation following of the human embryonic cortical stem cells[J].Hum Cell.2003.16(3):151-156.
279.ZhangS C.Embryonic stem cells for neural replacement therapy:prospects and chalenges.[J]Hematother Stem Cell Res.2003.12(6):625-634.
280.RipponH I,B ishopA E.Embryonic stem ce lls[J].Cell Prolif,.2004.37(1):23-34.
281.Lopez M oratalla N,Gonzalezd,et alI.Human plulrpotent stems cells[J].Rev Med Univ Navarra.,2003,47(3):34-42.
282.诸敏.邓志锋,汪决.干细胞移植在中枢神经系统疾病疗中的进展[J].国际脑血管病杂志,2007,15(3):224-226.
283.王金花.干细胞在脊髓损伤治疗中的研究进展[J].临床研究,2007,14(17):52-55.
284.张延洁,蔡芳,杨一峰.中老年人骨髓间质干细胞的生物学特性研究[J].中国生物工程杂志,2005,25(4):33-37.
285.雷俊霞,郭振宇,赵东长.大鼠骨髓间质干细胞对同种骨髓移植后造血重建和免疫重建的作用[J].中国病理生理杂志,2006,22(6):1129-1132.
286.Le Blanc K,Rasmusson I,Gotherstrom C,et al.Mesenchymal stem cells inhibit the expression of CD25(interleukin-2 receptor)and CD38 on phytohaemagglutinin-activated lymphocytes[J].Scand J Immunol,2004,60(3):307-315.
287.Frank MH,Sayegh MH.Immunomodulatory functions of mesenchymal stem ceils[J].Lancet,2004,363(9419):1411-1412.
288.王灯亮,康德智,林建华.间质干细胞修复脊髓损伤的理论与应用[J].中国临床康复,2006,10(17):155-157.
289.CHOPP M,ZHANGX H,LI Y,et al.Spinal cord injury in rat:treatment with bone marrow stromal cell transplantation[J].Neuoreport,2000,11(13):3001.
290.Li CH,Jiao BH.Effect of bone marrow stromal cells transfected with interleukin 18 on growth of intracranial glioma in rats[J].Ai Zheng,2007,26(1):38-43.
291.Chert J,LiY,Katakowski M,et al.Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat[J]..Neurosci Res,,2003,73:778-786.
292.张珊红,郭亮,龚卫琴.骨髓间充质干细胞在脑缺血再灌注大鼠脑内存活并分化为神经元样细胞的实验研究[J].中国老年学杂志,2007,27(19):1871-1873.
293.王颖,邓宇斌,李艳.骨髓间质干细胞诱导为神经元样细胞移植治疗脑梗塞模型大鼠后运动功能的改善[J].中国病理生理杂志,2006,22(12):2401-2406.
294.刘晓刚,邓宇斌.神经元样细胞与控释神经营养因子联合移植对脊髓损伤猴后索结构修复和功能恢复的作用[J].中国临床康复,9(26):96-99.
295.程伟,邬伟,郑国庆.中药诱导干细胞分化为神经元样细胞的研究思路和方法[J].中华中医药学刊,2007,25(7):1403-1405.
296.杨晓苏,吴海香,肖波.人骨髓间质干细胞分化为神经元样细胞及运动神经元生存蛋白表达的研究[J].中华医学杂志,85(16):1125-1127.
297.罗新明,杨晓苏,肖波.丙戊酸对脊髓性肌萎缩症患者神经元样细胞SMN2基因mRNA表达的影响[J].中华神经科杂志,2006,69(3):159-161.
298.Lou S,Gu P,Chen F,et al.The effect of bone marrow stromal cells on neuronal differentiation of mesencephalic neural stem cells in Sprague-Dawley rats[J].Brain Res,2003,968(1):114-121.
299.王斌,罗毅文,胡年宏.骨康方含药血清诱导大鼠骨髓基质细胞向成骨细胞方向分化的实验研究[J].中国中医骨伤科杂志,2007,15(11):31-33.
300.杨丽.OP模鼠MSCs生物学特性及药物对大鼠MSCs骨向分化的干预研究.暨南大学2007年博士学位论文,101-102.
301.史丙俊.中药血清药理学研究初探[J].泸州医学院学报,2003,26(3):272-273.
302.#12
303.冯瑜娟,邓翀,孟宪丽.血清药物化学应用于中药复方配伍研究的思路[J].中药药理与临床2007,23(5):261-263.
304.齐炼文,李萍,赵静.代谢组学与中医药现代研究[J].世界科学技术-中医药现代化,2006,(06):177-179.
305.黄敏,杨坤杰.中药血清药理学的研究方法及其改进思路[J].湖北中医杂志,2006,28(5):24-26.
306.米永杰,李健.中药血清药理学研究概述[J].四川解剖学杂志,2006,14(4):34-36.
307.王睿冲药血清药理学研究进展[J].齐齐哈尔医学院学报,2006,27(18):2243-2244.
308.罗焕敏.“血清药理学”与“血浆药理学”[J].中国药理学通报,2003,19(9):1075-1076.
309.陆慧晶.血清药理学研究中的若干问题探讨[J].基层中药杂志,2000,14(3):51.
310.崔玲,晏卫东.中药血清药理研究方法回顾与展望[J].武警医学,2004,15(6):460-461.
311.李仪奎.中药血清药理学实验方法的若干问题[J].中药新药与临床药理,1999,10(2):95-96.
312.王宁生,杨奎,雷燕,等.关于血清药理学的若干思考[J].中国中西医结合杂志,1999,19(5):263.
313.王力倩,李仪奎.血清药理学方法研究探索[J].中药药理与临床,1997,13(3):29-31.
314.梁春敏,王贤喜,董群,等.玉屏风散对小鼠免疫调节作用的血清药理学研究[J].上海免疫学杂志,2003,23(6):285-287.
315.赵明奇,吴伟康,段新芬,等.四逆汤对阿霉素心衰性大鼠线粒体功能的影响[J].中药材,2005,28(6):486-488.
316.Yang YF,Wang YQ.Study on standardization of serum pharmacologic study on Chinese composite recipes[J].Chin J Integrated Tradit West Med,2000;20(5):380-381.
317.杨仙凌.中药血清药理学研究方法应用现状及展望[J].浙江中医药大学学报,2007,31(5):662-665.
318.熊鹰.孔小云,陈如山,等.复方犀黄丸含药血清体外抗癌作用的研究[J].中国中西医结合消化杂志,2001;9(4):82.
319.吴健宇,穆静,李仪奎,等.血清药理学方法体外实验系统的血清添加量问题[J].上海中医药杂志,2001;(9):8-11.
320.王睿.药血清药理学研究进展[J].齐哈尔医学院学报,2006,27(18):2243-2246.
321.孟凡刚,吴承远.丹参诱导成年小鼠骨髓基质干细胞分化为神经细胞研究[J].上海中医药杂志,2005,39(10):48-49.
322.杨立业,刘湘名,惠国桢,等.丹参诱导鼠骨髓间充质细胞向神经元分化[J].中华儿科杂志,2003,41(8):607-610.
323.李爱秀.中药“药效团药性假说”的提出[J].天津药学,2007,19(2):42-44.
324.王喜军,张宁,常存宽.方剂配伍规律的研究现状和未来发展[J].世界科学技术-中医药现代化,2006,8(4):13-15.
325.黄敏,杨坤杰.中药血清药理学的研究方法及其改进思路[J].湖北中医杂志,2006,28(5):24-26.
326.李萍,刘惠如,黄涛.中药血清药物化学对中医现代化的意义[J].传统医药,2007,16,(2):58.
2.Pittenger MF,Mackay AM,Jaiswal SC,et al.Multilineage potential Of adult human mesenchymal stem cell[J].Science,1999,284(5411):143.
3.Woodbury D,Schwarz EJ,Prockop DJ,et al.Adult rat and human bone marrow stromal cells differentiatein to neurons[J].J Neurosci Res,2000,61(4):364.
4.胡向阳,张荣华.中药诱导骨髓间充质干细胞向神经样细胞分化研究现状与探讨[J].辽宁中医杂志,2007,34(11):1657-1658.
5.张本斯,王凡,邓力.大鼠骨髓间充质干细胞分离培养及表型和功能特点[J].四川大学学报(医学版),2003,34(4):738-741.
6.Friedenstein AJ,Gorskaja JF,Kulagina NN.Fibroblast precursors in normal and irradiated mouse hematopoietic organs[J].Exp Hematol.1976,4(5):267-274.
7.Castro Malaspina H,Gay RE,gesnick G.et al.Characterization of human bone marrow fibroblast colony-forming cells and their progeny[J].Blood,1980,56:289-301.
8.Conget PA,Minguell JJ.Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells[j].Cell Physiol,1999,181:67-73.
9.Friedenstein AJ.Osteogenic stem cells in the bone marrow.Bone and Mineral Research[J],1990,7:243-272.
10.Prockop DJ.Marrow stromal cells as stem cells of nonhematopoietic tissues for continual renewal and as potential vectors for gene therapy[J].Cell Biochem Suppl,1998,30-31:284-285.
11.Deans RJ,Moseley AB.Mesenchymal stem cells:biology and potential clinical uses[J].Exp Hematol.2000,28(8):875-884.
12.Noth U,Full R,Osyczka A M,et al.In vitro engineered cartilage constructs produced by presscoating bio-degradable polymer with human mesenchymal stem cells[J].Tissue Eng,2002,8(1):131-144.
13.Friedenstein A J.Osteogenic stem cells in the bone marrow[J].Bone and Mineral Research,1990,7:243-272.
14.J.E.Dennis,J.P.Carbillet,A.I.Caplan,et al.TheSTRO-1+ marrow cell population is multipotential[J].Cells Tissues Organs,2002,170:73-82.
15.Tondreau T,Lagneaux L,Dejeneffe M,et al.Isolation of mesenchymal stem cells by plastic adhesion or negative selection:phenotype,proliferation kinetics and differentiation potential.[J].Cytotherapy,2004,6(4):372-379.
16.Mizobe T,Kidoguchi K,Tamaki M,et al.Sca-1 and Thy-1 accelerate neuron-like differentiation in bone marrow stromal cells[J].Kobe J Med Sci,2006,52(5):119-125.
17.Quirici N,Soligo D,Bossolasco P,et al.Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factorreceptor antibodies[J].Exp Hematol,2002,30(7):783-789.
18.Gurevich O,Vexler A,Marx G,et al.Fibrin Microbeads for Isolating and growing bone marrow-derived progenitor cells capable of forming bone Tissue[J].Tissue Eng,2002,8(4):661-672.
19.Baddoo M,Hill K.Willinson R,et.al.Characterization of mesenchymal stem cells isolated from murine bone marrow by negative Selection.[J].Cell Biochem,2003,89(6):1235-1249.
20.Zvailer NJ,Man'nova ML,Adams G,et al.Mesenchymal precursor cells in the blood of normal individuals[J].Arthritis Res,2000,2(6):477-479.
21.Erices A,Conger P,Minsuell JJ.Mesenchymal progenitor cells in human umbilical cord blood[J].Br J Haematol,2000,109(1):235-237.
22.Zuk PA,Zhu M,Mizuno H,et al.Multi—lineage cells human adipose tissue:implications for cell based therapies[J].Tissue Eng,2001,7(2):211-213.
23.Kern S,Eichler H,Stoeve J,et al.Comparative analysis of mesenchymal stem cells from bone marrow,umbilical cord blood,or adipose tissue[J].Stem Cells,2006,24(5):1294-1301.
24.Neort WA,KmissdbrinkAB,AnkerPS,etal.Mesenchyreal stem cells promote engraftment of human umbilical cord blood derived CD34 cells in NOD/SCID mice[J].Exp Hematol,2002,30(8):870.
25.Bang Y,Vaessen B,Lenvi KT.et al.Multi—potent progenitor cells can bc isolated from post natal marine bone marrow,muscle,and brain[J]..Exp Hematol,2002,30(8):896.
26.Almeida PG,EIShabrawy D,Porada C,et al.Diferentiative potential of human metanephric mesenchymal cells[J]..Exp Hematol,2002,30(12):1454.
27.Bruder SP,Jaiswal N,Haynesworth SE,et al.Growth kineties,self-renewal,and the osteogenie potential of purified human mesenehymal stem cells during extensive subeultivation following cryoprcservation[J].Cell Bio chem,2003,64(2):278-294.
28.Lennon DP,Haynesworth SE,Young RG,et al.A chemically defined medium supports in vitro Proliferation and maintains the osteochondral potential of rat marrow derived mesenehyma Istem cells[J]..Experimental Cell Research,2005,219(1):211-222.
29.Tsutsumi S,Noel B,Flautre B,et al.Assoeiation of porous hydroxyapapite and bone marrow cells for bone regeneration[J].Bone,1999,25(3):515-545.
30.李建华,黄建荣,樊雪萍,等.胰岛素样生长因子-I对人骨髓间充质干细胞增殖的影响[J].中国临床康复,2004,8(8):1464-1466.
31.杨莉,王冬梅,洪欣,等.成人骨髓间充质干细胞体外扩增和定向诱导分化为骨和软骨细胞的研究[J].自然科学进展,2001,10:25-42.
32.Friedemten AJ,Dglllllo JF,Kulagina NN.et al.Precursors for fibroblast in different population Of hematopoitie cells as deteced by the in vitro colony assay method[J].Exp Hemat,1974,2(2):83-92.
33.姚晓黎,张成,卢锡林等.成人骨髓间质干细胞体外分化为神经元样细胞[J].广东医学,2004,25(5):504-506.
34.JIANG Y,JAHAGIRDAR BN,REINHARDT RL.Pluripoteney of mesenchymal stem cells derived from adult marrow[J].Nature,2002,418(6893):41-49.
35.Conger PA,Minguell JJ.Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells[J].Cell Physiol,1999,181:67-73.
36.路艳蒙,傅文玉,朴英杰.人骨髓间充质干细胞的超微结构[J].电子显微学报,2002,04:373-374.
37.李善宗,张成,吴金浪,等.体外培养大鼠骨髓间质干细胞的超微结构[J].中山大学学报(医学科学版),2003,24(1):11-15.
38.Song L,Tuan RS.Transdifferentiation potential of human mesenchymal stem cells derived from bone marrow[J].FASEBJ,2004,18(9):980-982.
39.Bruder SP,Jaiswal N,Haynesworth SE.Growth kinetics,self-renewal,and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation.[J].Cell Biochem,2005,64(2):278-94.
40.Bianchi G,Banfi A,Mastrogiacomo M,Notaro R,et al.Ex vivo enrichment of mesenehymal cell progenitors by fibroblast growth factor 2[J].Exp.Cell Res,2003,287:98-105
41.Simonsen JL,Rosada C,Serakinei N,et al.Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrowstromal cells[J].Nat Biotectmol,2002,20:592-596.
42.Shi S,Gronthos S,Chen S,et al.Bone formation by human postnatal bone marrow stromal stem cells is enhanced by telomerase expression[J].Nature Biotechnol.2002,20:587-591.
43.D.Baksh,L.Song,R.S.Tuan.Adult mesenchymal stem cells:characterization,differentiation,and application in cell and gene therapy[J].JCell Mol Med,2004,8(3):301-316.
44.Lange C,Bruns H,Kluth D,et ai.Hepato-cytic differentiation of mesenchymal stem cells in coc-ultures with fetal liver cells[J].World J Gastroenterol,2006,12(15):2394-2397.
45.Chen LB,Jiang XB,Yang L.Differentiation of rat marrow mesenehymal stem cells into pancreatic islet betacells[J].World J Gastroenterol,2004,15,10(20):3016-3020.
46.Wislet-Gendebien S,Hans G.Leprince P,et al.Plastieity of cultured mesenchymai stem cells:switch from nestin-positive to excitable neuron-like phenotype[J].Stem Cells,2005,23(3):392-402.
47.裴雪涛.干细胞技术[M].化学工业出版社,2002,第一版:36.
48.Chopp M,Zhang XH,Li Y,et al.Spinal cord injury in rat:Treatment with bone marrow stromal cell transplantation[J].Neuroreport,2000,11(13):3001-3005.
49.Jiang W,Ma A,Wang T,et al.Homing and differentiation ofmesenchymal stem cells delivered intravenously to ischemic myocardium in vivo:a timeseries study.P flugers[J].Arch,2006,453(1):43-52.
50.Koji Natsu,Mitsuo Ochi,Yu Mochizuki,et al.allogeneie bone-derived mesenchymal stromal cells promote the regeneration of injured skeletal muscle without differentiation into myofibers,tissue[J],engineering,2004,10:1093-1099.
51.Chen J,Li Y,Wang L,et ai.Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats[J].Stroke,2001,2(4):1005-1011.
52.LeBlanc K.Mesenchymal stromal cells:Tissue repair and immune modulation[J].Cyto therapy,2006,8(6):559-561.
53.Tse WT,Pendleton JD,Beyer WM,et al.Suppression of logeneic T-cell proliferation by human marrow stromal cells:implications in transplantation[J].Transplantatien,2003,75:389-397.
54.McIntosh KR,Bartholomew A.Stromal cell modulation of the immune system:a potential role for mesenchymal stem cells[J].Graft,2000,3:324-328.
55.Corcione A,Benvenuto F,Ferretti E.Human mesenchymal stem cells modulate B cell functions[J].Blood,2006,107(1):367-372.
56.Le Blanc K,Tammik L,Sundberg B,et al.Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures andmitogenic responses independently of the major histocompatibility complex[J].Scand J Immunol,2003,7:11-20.
57.Di Nicola M,Carlo-Stella C,Magni M,et al.Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli[J]..Blood,2002,99:3838-3843.
58.WEXLER SA,DNALDSON C,DENNING KENDAII F,et al.Adult bone marrow is a rich source of human mesenchymal stem cells but umbilical cord and mobilized adult blood are not.[J].Haematol,2003,121:368-374.
59.MINGUEII JJ,ERICES A,CONGET P..Mesenehymal.stem cells[J]..Exp Biotrices Meal,2001,226:507-520.
60.Minguell,Alejandro Edces,Paulette Conget.Mesenchymal Stem Cells[J].Experimental Biology and Medicine,2001,226:507-520.
61.Bianco P,Riminucci M,Gronthos S,et al.Bone marrow stromal stem cells:nature,biology,and potential applications[J]..Stem Cells,2001,19(3):180-192.
62.Martinez C,Hofmann TJ,Marino R,et al.Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2:a novel surface marker for the identification of MSCs[J].Blood,2007,30:336-338.
63.DBaksh L.Song RS.Tuan.Adult mesenchymal stem cells:characterization,differentiation,and application in cell and gene therapy[J].Cell Mol,2004,8(3):301-316.
64.连霞,李光来.骨髓间充质干细胞生物学特性及其多向分化潜能的理论基础和机制[J].临床医药实践杂志,2006,15(3):163-165.
65.林建华,雷盛民,康德智,等.静脉注骨髓间质干细胞对脊髓损伤修复作用的实脸研究[J].中华骨科杂志,2005,25(9):556-558.
66.Vulliet PR,Greeley M,Halloran SM,et al.Intra-coronary arterial injection of mesenchymal stromal cells and microinfarction in dogs[J].Lancet,2004,363(9411):783-784.
67.Lu L,Zhao C,Liu Y,et al.Therapoutic benefit of TH-engineered mesenchymal stem cells for Parkinson's disease[J].BrainRes Brain Res Protocol,2005,15(1):46-51.
68.Mimura T,Dezawa M,Kanno H,et al.Peripheral nerve regeneration by transplantation of bone marrow stromal cell-derived Schwann cells in adult rats[J].J Neuro surg,2004,101(5):806-812.
69.SAKAI D,MOCHIDA J,YAMAMOTO Y,et al.Tranlantation of mesenchymal stem cells embedded in Aieloeollagen gel tothe inter-vertebral disc:a potential therapeutic model for disc degeneration [J].Biomaterials,2003,24(20):3531-3541.
70.Woo SL,Hildebrand K,Watanabe N,et al.Tissue engineering of ligament and tendon healing[J].Clin Orthop.2006,12(11):312-323.
71.Jorgensen C,Djouad F,Apparailly F,et al.Engineedng mesenchymal stem ceils or immuao-therapy[J].Gene Ther,2003,23(10):928-931.
72.Woodbury D,Schwarz EJ,Prockop DJ,et al.Adult rat and human bone marrow stromal cells differentiate into neurons[J].J Neurosci Res,2000,61(4):364-370.
73.Deng W,Obrocka M,Fischer I,et al.In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intraccllular cyclic AMP[J].Biochcm Biophys Res Commun,2001,282(1):148-152.
74.项鹏,夏文杰,张丽蓉,等.成人骨髓间质干细胞定向诱导为神经元样细胞的研究[J].中国病理生理杂志,2001,17(5):385-387.
75.张卉,王纪佐,孙红宇,等.成鼠骨髓基质细胞分化成神经元的体外研究[J].脑与神经疾病杂志,2001,9(6):336-338.
76.李德华,单伟,张海锋.体外诱导人脐静脉间充质干细胞分化为神经元样细胞的实验研究[J].神经解剖学杂志,2007,23(5):554-558.
77.辛颖,李玉林,张丽红.成人骨髓间充质干细胞体外定向诱导分化为神经元样细胞的研究[J].中国实验诊断学2007,11(1):4-6.
78.施雪英,陈先文,任于果.大鼠骨髓间充质干细胞在体外向神经元样细胞的分化[J].安徽医科大学学报,2006,41(1):16-19.
79.Black I,Woodbury D..Adult rat and human bone marrow strom stem cells differentiate into neurons[J].Blood Cells MolDis,2001,27:632-636.
80.Kohyama J,Abe H,Shimazaki T,et al.Brain from bone:efficient "meta differentiation"of marrow stromaderived mature osteoblasts to neurons with noggin or ademethylating agent[J].Differentiation,2001,68:235-244.
81.Sanchez-Rarnos J,Song S,Cardozo-Pelaez F,et al,Adult bone marrow stromal cells diferentiate into neural cells in vitro[J].Exp Neurol,2000;164(2):247-256.
82.徐如祥,戴宜武,姜晓丹,等.成年骨髓间质干细胞体外诱导分化成神经细胞研究[J].中华神经外科疾病研究杂志,2002,1f1):63-67.
83.许期年,金钧,苗宗宁.脑源性神经生长因子促进骨髓间充质干细胞分化为神经元样细胞的实验研究[J].苏州大学学报(医学版)2007,27(4):520-522.
84.梅晰凡,王伟,刘品端.碱性成纤维细胞生长因子和神经生长因子联合诱导成年鼠骨髓基质细胞向神经元样细胞的分化[J].中国临床康复,2006,10(13):32-35.
85.叶民,陈生弟,陆国强,等.成年大鼠骨髓基质干细胞诱导分化为神经元样细胞不同方法比较的研究.[J]中国神经科学杂志.2004,20(4):275-281.
86.钟春玖,王志军,金莉蓉.诱导骨髓基质细胞表达神经细胞标记物Nestia,NSE和GFAP:脑源性神经节苷脂定向诱导细胞分化作用的研究[J].中国临床医学,2005,3(5):761-762.
87.Quirici N,Soligo D,Bossolasco P,et al.Isolation of bone marrow mesenchymal stem cells by anti—nerve growth factor receptor antibodies[J].Exp Hematol,2002;30(7):783-791.
88.Satomura K,Derubeis A,Fedarko NS,et ol.Receptor tyrosine kinase expression in human bone marrow stromal cells[J].Cell Physical,1998:177(3):426-438.
89.Tokumine J,Kakinohana O,Cizkova D,et ol.Changes inspinal GDNF,BDNF and expression after transient spinal cord ischemia in the mice[J].Neurosci Res,2003:74(4):552-561.
90.Tassi E,AlAttar A,Aigner A,et ol.Enhancement of fibroblast growth factor(FGF)activity by an FGF—binding protein[J].Biol Chem,2001;276(43):40247-40253.
91.黄慧,唐云安,张成.香丹注射液定向诱导大鼠骨髓间质干细胞分化为神经元样细胞[J].中药材,2004,27(8):585-589.
92.刘金保,董晓先,董燕湘等.当归诱导骨髓间充质干细胞分化为神经元样细胞[J].广东医学,2003,24(5):466-468.
93.赵楠,冯忠堂,王廷华等.丹参注射液诱导骨髓基质细胞分化为神经元的研究[J].昆明医学院学报,2004,25(4):27-29.
94.黄洁,张宜,杜少辉等.牛珀至宝微丸对骨髓间充质干细胞移植后转分化为神经元的影响.[J]中华中医药杂志,2005,20(12):721-723.
95.董晓先,刘金保,董燕湘等.天麻诱导骨髓间质干细胞分化为神经元细胞的实验研究[J].中国中西医结合杂志,2004,24(1):51-54.
96.张进,徐志伟.补肾法诱导间充质干细胞向神经方向分化研究[J].现代医院,2004,4(9):15-17.
97.王勇,陆长青,王凡.黄芪诱导大鼠骨髓间充质干细胞分化为神经样细胞的研究[J].四川解剖学杂志,2006,14(1):5-8.
98.陈东风,杜少辉,李伊为等.龟板含药血清体外诱导成年大鼠骨髓间充质干细胞分化为神经元[J].广州中医药大学学报,2003,20(3):224-226.
99.陆长青,王勇,陈登榜等.丹参诱导骨髓基质干细胞向神经元样细胞分化及相关基因的表达[J].四川解剖学杂志,2006,14,(1):1-4.
100.董燕湘,董晓先,何慧华等.大鼠骨髓间质干细胞用中药绞股蓝诱导为神经细胞的研究[J].中华神经科杂志,2003,36(5):355-358.
101.撒亚莲,李海标.川芎嗪诱导大鼠骨髓间质干细胞分化为神经元样细胞的研究[J].解剖学报,2003,34(5):515-518.
102.余勤,罗依,鄂艳等.丹参素定向诱导骨髓间质干细胞分化为神经元样细胞的研究[J].中国中西医结合杂志,2005,25(1):49-54.
103.蔡光先,林琳刘柏炎等.地黄多糖诱导骨髓间充质干细胞分化为神经样细胞的效应[J].中国临床康复,2005,9(17):17-19.
104.项平,李海标.黄连素诱导大鼠骨髓间质干细胞分化为神经元样细胞[J].中国病理生理杂志,2004,20(1):51-53.
105.贾延劼,杨于嘉,周燕等.黄芩甙体外诱导大鼠骨髓基质细胞成为神经细胞[J].中华医学杂志,2002,82(19):1337-1342.
106.唐云安,王瑞淑,张成.大鼠脊髓匀浆上清液对骨髓间质干细胞的诱导分化作用[J].中风与神经疾病杂志,2003,20(3):196-199.
107.宋永周,崔慧先,吕哲,等.创伤性脑组织匀浆对大鼠骨髓间充质干细胞分化为神经元样细胞的影响[J].中国组织工程研究与临床康复,2008,12(3):461-463.
108.牛朝诗,孔杰.损伤脑组织匀浆诱导骨髓间质干细胞向神经细胞分化的研究[J].立体定向和功能神经外科杂志,2006,19(5):270-272.
109.张燕青,曾园山,张静.体外培养的嗅鞘细胞能够促进骨髓间充质干细胞分化为神经元样细胞[J].中国神经精神病杂志,2007,33(10):611-614.
110.牛云,何旭,王心蕊.鼠胶质瘤细胞上清液诱导人骨髓间充质干细胞向神经元样细胞的分化[J].吉林大学学报(医学版),2007,33(4):635-637.
111.陈宁,李昌琪,罗学港.骨髓基质干细胞诱导转化与移植[J].国外医学·生理、病理科学与临床分册,2002,23(5):534-537.
112.闰彬彬,王莉莉,乔俊峰等.体外诱导骨髓基质干细胞向神经元分化的机制研究[J].细胞与分子免疫学杂志[J],2005,21(3):301-304.
113.Denrus J E,Charbord P.Origin and differentiation of Human and Murine Stroma[J].Stem cells,2002,20(3):205-214.
114.高健,刘振华,姜晓丹等.骨髓源性神经干细胞诱导分化为多巴胺能神经元的实验研究.中华神经医学杂志[J],2006,5(4):349-352.
115.Yamaguchi A,Komori T,Suda T.Regulation of osteoblast differentiation mediated by bone morphogenetic proteins,hedgehogs,and Cbfal[J].Endocrine Rev,2000,21(4):393-411.
116.Deng W,Obrocka M,Fischer I,et al.In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracell ularcyclic AMP[J].Biochem Bioph Res Co,2001,282(1):148-152.
117.王海燕,徐如祥,罗深秋等.多细胞因子诱导成年鼠骨髓基质细胞向神经细胞分化的实验研究[J].中国微侵袭神经外科杂志,2004,9(2):77-81.
118.Sanchez-Ramos J,Song S,Cardozo-Pelaez F.et al.Adult bone marrow stromal cells differentiate into neural cells in vitro[J].Exp Neurology,2000,164(2):247-256.
119.Woodbery D,Reynolds K,Black I B.Adult bone marrow stromal stem cells express germline,ectodermal,endodermal,and mesdermal genes prior to neurogenesis[J].Neurosci Res,2002,69(6):908-917.
120.Weslet-Gendebien S,Hans G,Leprince P,et al.Plasticity of cultured mesenchymal stem cells:switch from nestin-positive to excitable neuron-like phenotype[J].StemCells,2005,23(3):392-402.
121.Jori FP,Melone MA,Napolitano MA,et al.RB and RB2/PBO genes demonstrate both specific and overlapping functions during the early step of in vitro neural differentiation of marrow stromal stem cells[J].Cell Death Differ,2005,12(1):65-77.
122.Jori FP,Napolitano MA,Melone MA,et al.Molecular pathways involved in neural in vitro differentiation of marrow stromal stem cells[J].J Cell Biochem,2005,94(4):645-655.
123.冯英,谢畜康等.骨髓基质干细胞诱导成神经元样细胞分化相关基因的表达[J].中国病理生理杂志,2004,20(12):2268-2271.
124.Shafit-Zagardo B,Kalcheva N.Making sense of the multiple MAP-2 transcripts and their role in the neuron[J].Mol Neurobiol,2001,16(2):149-162.
125.Kaech S,Parmar H,Roelandse M,et al.Cytoskeletal microdifferentiation:a mechanism for organizing morphological plasticity in dendrites[J].Proc Natl Acad Sci USA,2003,98(13):7086-7092.
126.任宁,王新平,张文治.骨髓间充质干细胞向神经细胞分化的研究进展[J].国际脑血管杂志,2006,14(1):65-68.
127.高晓山.病理生理条件下中药十八反实验研究的综合报告[J].中医杂志,1991;(1):36-38.
128.关天增,潘先琼,常洪志.等.“诸参辛芍叛藜芦”之实验研究[J].河南中医,1993;13(5):204-205.
129.金恩波,姜名瑛,黄启福,等.中药十八反的药理研究[J].中成药研究,1982;(2):28-31.
130.《中华人民共和国药典》[M].国家药典委员会编.北京:化学工业出版社,2005:199.
131.李同琴,郭秋红,仝利琪.党参反藜芦的动物实验研究.陕西中医,2003,23(8):744.
132.杨胜辉,刘威.十八反中几组药物配伍的临床应用.陕西中医,2000,21(4):179.
133.《中华人民共和国药典》.国家药典委员会编.北京:化学工业出版社,2005:52-53.
134.LAYI S,HSIEH CC,CHIUJ H,et al.Salvianolic acid B enhances in vitro angiogenesis and imp roves skin flap survival in Sprague-Dawley rats[J].Surg Res,2003,115(2):279-286.
135.GES R,YUY X,XIEG X.Advances in studies on pharmacological activities of salvianolic acids[J].Chin M ed Material,2002,25(9):683-685.
136.L IANG Y,YANG YM,YUANS L.Advances in studies on pharmacological activities and clinical applications of Tanshinones[J].Chin Tradit Herb D rugs,2000,31(4):304-306.
137.赵娜,郭治昕,赵雪.丹参的化学成分与药理作用[J].国外医药·植物药分册,2007,22(4):155-157.
138.杜冠华,张均田.丹参现代研究概况与进展[J].医药导报,2004,23(6):355-361.
139.叶勇.丹参有效成分分离的研究进展[J].药品评价,2005,2(2):146-148.
140.祖淑玉,高咏梅,朱广瑾.丹参对血管紧张素诱导内皮细胞组织因子基因的表达的作用[J].中华老年心血管病杂志,2004,6(4):257-260
141.Ding M,Zhao G R,Ye T X,et al.Salvia miltiorrhiza protect sendothelial ceils against oxidative stress[J].Altem Complement Med,2006,12(1):5-6.
142.Ling S H,Dai A Z,Guo Z X,et al.Effects of a Chinese herbal preparation on vascular cells in culture:mechanisms of cardiovascular protection[J].Clin Exp Pharmacol Physiol,2005,32(7):571-578.
143.孙可青,徐长庆.丹参素的抗心律失常作用及其电生理机制的研究[J].中国中医药科技,2000,7(3):171-172
144.徐长庆,王孝铭,范劲松,等.丹参酮Ⅱ-A对豚鼠单个心室肌细胞跨膜电位及L-型钙电流的影响[J].中国病理生理杂志,1997,13(1):43-47.
145.Chang P N,Mao J C,Huang S H,et al.Analysis of cardioprotective effects using purifiedSalvia miltiorrhiza extract on isolated rat hearts[J].Pharrnacol Sci,2006,101(3):245-249
146.史举彤,杨文浩,陈长清.丹参素对体外循环中血细胞的影响[J].山东医科大学学报,1991,29(3):237-239.
147.Imanshahidi M,Hosseinzadeh H.The pharmacological effects of Salviaspecies on the central nervous system.[J].Phytother Res,2006,20(6):427-437
148.刘军,匡培根,吴卫平,等.大鼠脑缺血再灌注区小胶质细胞反应及丹参的影响[J].中华老年心脑血管病杂志,2002,4(2):127-129
149.沈霞,崔桂云,张璐,等.丹参对大鼠全脑缺血再灌注后海马CA1区AP1DNA结合活性的影响[J].江苏医药杂志,2004,30(6):422-424.
150.Lao C J,Lin J G,Kuo J S,et al.Effect ofSalvia miltiorrhiza bunge on cerebral infarct in ischemia-reperfusion injured rats[J].Am J Chin Med,2003,31(2):191-200.
151.董静,罗桂林,苗维纳,等.丹参对实验性肺纤维化小鼠病理变化和核因子κB表达的影响[J].中国药理学通报,2003,19(12):1428-1431.
152.姜慧卿,王占魁,张晓岚,等.丹参单体IH764-3抗胆总管结扎大鼠胶原降解作用及其机制[J].胃肠病和肝病学杂志,2003,12(4):336-338.
153.吴杲,何招兵,吴汉斌.丹参酮的药理作用研究进展[J].现代中西医结合杂志,2005,14(10):1382-1385.
154.Soung do Y,Rhee S H,Kim J S,et al.Peroxynitrite scavenging activity of lithospermate B fromSalvia miltiorrhiza[J]..Pharm Pharmacol,2003,55(10):1427-1432
155.Ding M,Ye T X,Zhao G R,et al.Aqueous extract of Salvia miltiorrhizaattenuats increased endothelial permeability induced by tumor necrosis factor-α.[J].Int Immuno pharm,,2005,5(11):1641-1651.
156.Kwon H J,Hyun S H,Choung S Y.Traditional Chinese medicine improves dysfunction of peroxisome proliferators activated receptor alpha and mierosomal triglyeeride transferprotein on abnormalities in lipid metabolism in ethanolfed rats[J].Biofactors,2005,23(3):163-176.
157.汤建,李慧梁,黄海强.藜芦属植物化学成分的研究近况[J].药学进展,2006.30(5):206-209.
158.赵录英,赵画晨,吴博威.藜芦混碱对离体心泵功能的效应及其与洋地黄类药物的对比[J].山西医科大学学报,2002,33(3):231-232.
159.康毅,王国祥.牛磺酸抗藜芦碱实验性心律失常作用[J].天津医药,1989,17(4):199-201.
160.中国药科大学、中国医药出版社.中药辞海(第四卷)[M].北京:中国医药科技出版社,1998:8-13.
161.李淑媛,李卫平,李传勋.盐酸乌苏里藜芦碱对麻醉犬血流动力学的研究[J].中国药学杂志,1997,32(7):407-409.
162.刘燕,高广猷,叶丽虹.光脉黎芦碱的降压作用[J].大连医学院学报,1991,13(4):68-71.
163.李华,高广猷,李淑媛,等.乌苏里藜芦碱对肾性高血压大鼠中枢儿茶酚胺能神经元的作用[J].中国药理学报,2000,21(1):23-28.
164.马丽焱,周远鹏,江京俐.狭叶藜芦碱甲对犬血流动力学的影响[J].中草药,1998,29(2):105-107.
165.李欣燕,任洪灿,韩国柱.乌苏里藜芦碱在大鼠的血液流变学作用[J].大连医科大学学报,2004,26(3):165-167.
166.李欣燕,韩国柱,张书文.乌苏里藜芦碱对血小板聚集及凝血与出血时间的影响[J].中草药,2004,35(11):1269-1272.
167.段建红,段玉斌,邢俊玲,等.藜芦碱致使大鼠背根神经节A类神经元产生触发性振荡[J].生理学报,2002,54(3):208-212.
168.雷革胜,朱俊玲,万业宏,等.小剂量藜芦碱诱发大鼠脑海马CA1区锥体神经元异常放电癫痫脑片模型的特征[J].中国临床康复,2005,9(25):238-239。
169.由广旭,周琴,李卫平.乌苏里藜芦碱对大鼠脑缺血-再灌注损伤的保护作用[J].中草药,2004,35(8):908-911.
170.雷革胜,朱俊玲,万业宏,等.小剂量藜芦碱诱发大脑海马CA1区锥体神经元异常放电癫痫脑片模型的特征[J].中国临床康复,2005,9(25):238-239.
171.郑善子,崔春权,刘永镇.藜芦乳膏对毛囊蠕形螨的体外杀虫作用[J].延边大学医学学报,2004,27(1):31-32.
172.杨素华,李东宁,孙健.藜芦乳膏治疗疥疮的疗效观察[J].辽宁中医杂志,2002,29(11):664.
173.李太一,申成华,郑善子,等.藜芦乳膏的皮肤毒性实验研究[J].延边大学医学学报,2004,27(2):118-120.
174.徐国钧.徐珞珊,王峥涛.常用中药材品种整理和质量研究(第四册)[M].福建:福建科学技术出版社,2001:121-178.
175.毛晓峰,史志诚,王亚洲.我国藜芦属植物研究进展[J].动物毒物学,2003,18(1-2):17-21.
176.王延章.重审十八反[M].北京:中国中医药出版社,1992:23-59.
177.陈馥馨.782个含十八反、十九畏内服成药方组成与主治分析[J].中国医药学报,1987,(2):26.
178.楼朱雄,杨建国,王健生,等.HPLC法测定藜芦配丹参后丹参酮ⅡA含量的变化[J].中药材,1993,16(10):32.
179.王宇光,高月,柴彪新,等.人参、藜芦合用对大鼠肝P450酶活性及mRNA表达的调控作用[J].中国中药杂志,2004,29(4):366-370.
180.沈光稳·采用发光细菌研究探讨“十八反”配伍药物的临床毒性[J].中医药研究,1998,14(1):10.
181.Chem V,Yip B,Yam I,et al.Carrier incidence for spinal muscul aratrophy in Southern Chinese[J].Neurol,2004,251(9):1089-1093.
182.Yip B,Yam I,杜芳,et a.l.中国南方脊髓性肌萎缩的基因携带率[J].世界核心医学期刊文摘.神经病学分 册,2005,(2):26-27.
183.倪昊,王立文.脊髓性肌萎缩症及临床相关SMN基因的研究进展[J].国际儿科学杂志,2006,33(1):26-29.
184.Lefebvre S,Burlet P,Liu Q,et al.Correlation between severity and SMN Protein level in spinal muscul aratrophy[J].Nat Genet,1997,16(3):265-269.
185.Coovert DD,Le TT,McAndrew PE,et,al.The survival motor neuron protein in spinal muscular atrophy [J].Hum Mol Genet,1997,6(8):1205-1214.
186.Carter TA,Bonnermann CG,Wang CH,et al.A multicopy transcrip tion-repair gene,BTF P44,maps to the region and demonstrates SMA associated deletions[J].Hum Mol Genet,1997,6:229-236.
187.Scharf JM,Endrizzi MG,Wetter A,Huang S,et al.Identification of a candidate modifying gene for spinal muscular atrophy by comparative genomics[J].Nature Genet,2005,20:83-86.
188.Lcfebvre S,burglen L,Frezal J,et al.The role of SMN gene in proximal spinal muscular atrophy[J].HumMol Genet,1998,7:1531-1536.
189.邢晓为,傅俊江.脊髓性肌萎缩症的分子遗传学研究新进展[J].国外医学·生理、病理科学与临床分册,2001,21(6):438-450.
190.Monani UR,Lorson CL,Parsons DW,et al.A single nueleotide difference that alters splicing patterns distinguishes the SMA gene SMN1 from the copy gene SMN2[J].HumMol Genet,2003,8:1177-1183.
191.Lorson CL,Halmen E,Androphy EJ,Wirth B,et al.A single nueleotide in the SMN gene regulates splicing and is responsible for spinal muscular atrophy[J].Proc Natl Aead Sei USA,1999,96(11):6307-6311.
192.Coovert DD,Le TT,McAndrew PE,et,al.The survival motor neuron protein in spinal muscular atrophy[J].Hum Mol Genet,2006,6(8):1205-1214.
193.Lefebvre S,burglen L,Reboullet S,et al.Identification and characterization of spinal muscular atrophydetermining gene[J].Cell,1995,,80:167.
194.杨晓苏,张丽芳,肖波.SMN蛋白与脊髓性肌萎缩症的研究进展[J].中国神经科学杂志,2002,18(4):740-742.
195.Pellizzoni L,Charroux B,Dreyfuss G.SMN mutants of spinal muscular atrophy patients are defective in binding to snKNP proteins[J].Proc Natl Acad Sci USA,2003,96(20):11167-11172.
196.Li HMH,Chang JG,et al.The effect of inactivation of SMN gene on mouse[J].Nat Genet,2003,24:66-70.
197.Wirth B.An update of the mutation spectrum of the survival motor neuron gone(SMN1) in autosomal recessive spinal muscular atrophy(SMA)[J].Human Mutation,2001,15:228-237.
198.Wirth B,Herz W,Wetter A,Moskau S,et al.Quantitative analysis of survivalmotor neuron copies:identification of subtle SMN1 mutations in patientswith sp inal muscular atrophy,genotype-phenotype correlation,and imp lieation for genetic counseling[J].Am J Hum Gcnet,1999,64:1340-1356.
199.Feldkotter M,Schwarzer V,Wirth R,et al.Quantitative analyses of SMN1 and SMN2 based on real-time LightCyeler PCR:fast and highly reliable carrier testing and p rcdiction of severity of sp inalmnscular atrophy[J].Am J Hum Genct,2002,70:358-368.
200.Wirth B,Sehmidt T,P,,udnik-Sehoneborn S,et al.De novo rearrangements found in 2%index patientswith spinal muscular atrophy(SMA):mutation mechanisms,parental origin,mutation rate and implications for prenatal diagnosis[J].Cell,2004.,56:573-576.
201.Rossoll W,Jablonka S,Andreassi C,et al.Stun,the spinal muscular atrophy Determing gene product,modulates axon growth and localization of beta-actin mRNA in growth cones of moto-neurons[j].Cell Biol,2003,163(4):801-812.
202.Coovert DD,Le TT,McAndrew.PE,et al.The survival motorneuron protein in spinal muscular atrophy [J]..Mol Genet,1997,6:1205-1214.
203.倪昊,王立文.脊髓性肌萎缩症及临床相关SMN基因的研究进展[J]..国际儿科学杂志,2006,33(1):26-28.
204.Rossoll W,Jablonka S,Andreassi C,et al.Smn,the spinal muscular atrophy determing gene product,modulates axon growth and localization of beta2actinmRNA in growth cones of motoneurous[J].Cell Biol,2003,163(4):801-812.
205.Meister G,Buhler D,Laggerbauer B,et al.Characterization of a nuclear 20S complex containing the survival of motor neurons(SMN) protein and a specific subset of spliceosomal Sm proteins[J]...Hum Mol Genet,2000,9:1977-1986.
206.Campbell L,Hunter KM,Mohaghegh P,et al.Direct interaction of SMN with dp103,a putative RNA helicase:a role for SMN in transcription regulation[J].Hum Mol Genet,2000,9:1093-1100
207.Liu Q,Fischer U,Wang F,Dreyfuss G.The sp inalmuscular atrophy disease gene product,SMN and its associated protein SIPI are in complex with splice osomal snRNP proteins[J].Cell,2004,90:1013-1017.
208.Pagliardini S,Giavazzi A,Setola V,et al.Subcellular localization and axonal transport of the survival motor neuron(SMN) protein in the developing rat spinal cord[J]..Hum Mol Genet,2000,9:47-56.
209.Jones MA,McEwen IR,Hansen L.Use of power mobility for a young child with spinal muscular atrophy[J].Phys Ther,2003,83(3):253-262.
210.Cusco I,Barcelo MJ,Soler C,et al.prenatal diagnosis for risk of spinal muscular atrophy[J].BJOG,2002,109(11):1244-1249.
211.Migita M,Migita M,Uchikoba Y,et al.Genetic diagnosis of Werdnig Hoffmann disease:a problem for application to prenatal diagnosis[J].Nippon Med Sch,2003,70(1):45-48
212.Moutou C,Gardes N,Viville S.Duplex PCR for preimplantation genetic(PGD) of spinal muscular atrophy[J].prenat Diagn,2003,23(8):685-689.
213.Bergeijk J,Haastert K,Grothe C,et al.Valproic acid promotes neurite outgrowth in PC12 cells independent from regulation of the survival of motor neuron Protein.[J].Chem Biol Drug Des,2006,67:244-24.
214.Andreassi C,Jarecki J,Zhou J,et al.Aclarubicin treatment restores SMN levels to cells derived from type I spinal muscular atrophy patients[J].Hum Mol Genet,2001,10(24):2841-2849.
215.倪昊,王立文.脊髓性肌萎缩症及临床相关SMN基因的研究进展[J].国际儿科学杂志,2006,33(1):26-28.
216.Rossoll W,Jablonka S,Andreassi C,et al.Smn,the spinal muscular atrophy determing gene product,modulates axon growth and localization of beta2actinmRNA in growth cones of motoneurons[J].JCell Biol,2003,163(4):801-812.
217.Zhiming Yang,Yu-Jia Chang,I-Chen Yu,et al.ASC-J9 ameliorates spinal and bulbar muscular atrophy phenotype via degradation of androgen receptor[J].Nature Medicine,2007,13:348-353.
218.杨晓苏,吴海香,肖波.人骨髓间质干细胞分化为神经元样细胞及SMN蛋白表达的研究[J].中华医学杂志,2005,85:1121-1128.
219.罗新明,杨晓苏,肖波.脊髓性肌萎缩症患者神经元样细胞SMN2基因mRNA的表达[J].中华内科杂志,2006,45(10);831-833.
220.丁伟荣,吴晓牧,饶燕飞,等.人骨髓间充质干细胞体外分离培养方法的比较研究[J].江西医药,2007,42(2):115-117.
221.Jiang Y,Jahagirdar BN,Reinhardt RL,et al.Pluripotency of mesenchymal stem cells derived from adult marrow[J].Nature,2002,41841-49.
222.Friedenstein AJ,Piatetzky Shapiro Ⅱ,Petrakova KV.Osteogenesis in transplants of bone marrow cells.[J].Embryol Exp Morphol,1966,16(3):381.
223.Kuo CK,Tuan RS.Tissue engineering with mensenchymal stem cells[J].IEEE Eng Med Bio Mag,2003,22(5):51
224.Shin M,Yoshimoto H,Vacanti JP.In vivo bone tissue engineering using mesenchymal stem cells on a novel electrospun nano fibrous scaffold[J].Tissue Eng,2004,10(1-2):33
225.Pittenger MF,Mackay AM,Beck SC,et al.Multilineage potential of adult human mesenchymal stem cells[J].Science,1999,284(5411):143.
226.徐忠华,吴清玉.贴壁法分离培养大鼠骨髓间充质干细胞的生物学特性[J].中国组织化学与细胞化学杂志,2007,16(5):554-556.
227.代飞,吴军,许建中,等.两种从骨髓中分离人间充质干细胞方法的比较研究[J].第三军医大学学报,2005,27(16):1631
228.Coher DC,Clas R,DiGirolanao CM,et al.Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow[J].Proc Natl Acad Sci USA.2000,97(7):3213-3218.
229.陈欢意,牛平,赵帅,杜迎春.改良贴壁培养法简单的骨髓基质干细胞分离纯化法[J].解剖科学进展,2007,13(2):190-192.
230.Colter DC,Sekiya I,Prockop DJ.Identification of a subpopulation of rapidly self-renewing and mul-tipotential adult stem cells in colonies of human marrow stromal cells[J].Proc Natl Acad Sci USA.2001,98:7841-7845.
231.杨丽.OP模鼠MSCs生物学特性及药物对大鼠MSCs骨向分化的干预研究.暨南大学2004级博士学位论文,37.
232.Majumdar MK,Keane-Moore M,Buyaner D,et al.Characterization and functionality of cell surface molecules on human mesenehymal stem cells[J].Biomed Sci,2003;10(2):228-241.
233.Tropel P,Noel D,Platet N,et al.Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow[J].Exp Cell Res,2004,295(2):395-406.
234.HOR I Y,NAKAMURA T,KIMURA D,et al.Experimental study on tissue engineering of the small intestine bymesenchymal stem cell seeding[J].Surg Res,2002,102(2):156-160.
235.Devine SM.Mesenchymal stem cells:will they have a role in the clinic?[J].J Cell Biochem,2002;38[Suppl]:73-79.
236.Pittenger MF,Mackay AM,Jaiswal SC,et al.Multilineage potential of adult human mesenchymal stem cells[J].Science,1999;254(541):143-147.
237.杨丽.OP模鼠MSCs生物学特性及药物对大鼠MSCs骨向分化的干预研究.暨南大学2007年博士学位论文,101-103.
238.关宁,罗俊生,刘兴波,等.β-巯基乙醇和神经生长因子定向诱导鼠骨髓基质细胞分化为神经细胞的验证[J].中国组织工程研究与临床康复,2007,11(3):451-453.
239.龚理.NG对OP大鼠MSCs增殖、骨向分化及凋亡影响的实验研究.暨南大学2006年硕士学位论文,45-47.
240.Krause DS.Plasticity of marrow-derived stem cells[J].Gene Therapy,2002:9:754-758.
241.Erica L,Herzog,LiChai,et al.Plasticity of majrrow derived stem cells[J].Blood,2003,102(10):3483-3493.
242.李健,牛朝诗,傅先明.bFGF和N2体外联合定向诱导骨髓间质干细胞向神经元样细胞分化[J].立体定向和功能性神经外科杂志,2004,17(3):144-147.
243.陈宁,李昌琪,罗学港.骨髓基质干细胞诱导转化与移植[J].国外医学·生理、病理科学与临床分册,2002,23(5):534-537.
244.闰彬彬,王莉莉,乔俊峰等.体外诱导骨髓基质干细胞向神经元分化的机制研究[J].细胞与分子免疫学杂志,2005,21(3):301-304.
245.冯英,谢富康等.骨髓基质干细胞诱导成神经元样细胞分化相关基因的表达[J].中国病理生理杂志,2004,20(12):2268-2271.
246.张蒙夏,谢富康.成年SD鼠骨髓基质干细胞向神经元样细胞分化过程中分化与凋亡关系的研究[J].中国病理生理杂志,2003,19(1):32-35.
247.夏文杰,陈振光,张丽蓉等.隐丹参酮诱导骨髓间充质干细胞分化为神经元样细胞的实验研究[J]。中国中西医结合杂志,2002,22(12):921-924.
248.Wang LF,Zhao～HY.A theoretical investigation on DPPH radiealscavenging mechanism ofaravone[J].Bioorg Med Chem Lett,2003,13(21):3789-3792.
249.Takeda Y,Sato H,Satoh M,etal.Immunohistochemical expression of neural tissue markers(neuron-specific enolase,glial fibrillary acidic protein,S100 protein) in ameloblastic fibrodentinoma:a comparative study with ameloblastic fibroma[J].Pathol Int.2000,50(8):610-615.
250.Pekny M,Johnasson CB,Eliasson C,et al.Abnomral reation to eenrtal nervous system injury in mice lacking glial fibrillayr aeidic protein and vimentin[J].Cell Biol,1999,145(3):503-514.
251.Maslov AY,Barone TA Plunker RJ,et al.Neuarl stem cell detection,characterization,and age-related changes in the subvertrtieular zone of mice[J].Neuorsci,2004,24(7):1726-1733.
252.Kobayashi M,Sjobegr G,Sodehtall S,et al.Pediartic rhabdomyosacromas express the internrediate filament nestin[J].pediart Res,2005,43(3):386-392
253.KemPernrann G,Kronenberg G,et al.Early determination and long tens persistence of dault generated new neurons in hippoeampus mice[J].Development,2003,130(2):391-399.
254,Sanehez-Ramos JR,Cardozo-Pelaez F,Song S.Differentiation of neuron-like cells from bone marrow stromal cells[J].Mov Disord,1998,13(Suppl):122.
255.Woodury D,Schwarz EJ,Prockop DJ,et a.l Adult rat and human bone marrow stromal cells differentiate into neurons[J].Neurosei Res,2000,61(4):364-370.
256.Mezey Eva,Keys,VogelsangG,et a.l Transplanted bone marrow generates new neurons in human brains [J].PNAs,2003,100(3):1364-1369.
257.余勤,罗依,鄂艳,等.丹参素定向诱导骨髓间质干细胞分化为神经元样细胞的研究[J].中国中西医结合杂志,2005,25(1):49-54.
258.施雪英,陈先文,任于果,等.大鼠骨髓间充质干细胞在体外向神经元样细胞的分化[J].安徽医科大学学报,2006,41(1):16-18.
259.Ong SY,Dai H,Leong KW.Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture[J].Biomaterials 2006;27(22):4087-4097.
260.关宁,罗俊生,刘兴波,等.β-巯基乙醇和神经生长因子定向诱导鼠骨髓基质细胞分化为神经细胞的验证[J].中国组织工程研究与临床康复2007,11(3)2:451-454.
261.Majumdar MK,Thiede MA,Mosea JD,etal.Phenotypic and functional comparison of marrow derived mesenehymal stem cells[J].Cell physiol,2005,176(1):56-59.
262.Weiss S,Jehon Y.Pathways for neural stem cell biology and repair[J].Nat Bioteehnol,2004,17(9):850-851.
263.Sanehez Rarnos J.Adult bone marrow stromal cells differentiate into neural cells in vitro[J].Exp Neurol,2000,1 64:247-256.
264.Wurmser AE,Cage FH.Stem cell:Cell fusion causes confusion.[J]Nature,2002,416(6880):485-487.
265.Rietze RL,Valeanis H,Brooker CF,et al,Purification of a pluripotent neural stem cell from the adult mouse brain[J].Nature,2001,412(6848):736-739.
266.姚晓黎,张成,冯善伟,等.人骨髓间充质干细胞分化为神经元样细胞的逆转化现象[J].第一军医大学学报,2005,25(50:513-515.
267.DengW,Obroeka M,Fisher 1,et al.Adult bone marrow stromal cells differentiate into neural cells in vitro[J].Biochem Biophys Res Commun,2001,282:148-152.
268.Woodbury D,Reynolds K,Black IB.Adult bone marrow stromal stem cells express germline,ectodermal,endodermal,and mesodermal genes prior to neurogenesis[J].Neurosei Res,2002,96:908-917.
269.江和碧,卓本慧,代英等.bFGF促进大鼠骨髓间充质干细胞向神经元转化及机制[J].第三军医大学学报,2005,27(2):98-101.
270.Prockop DJ..Marrow stromal cell as stem cells for nonhematopoietis tissues[J].Science,1997,276(5309);71-74.
271.Vander Steege G,Draaijer TG,Groots cholten PM,et al.PCR-base DNA test to confirm the clinical diagnosis of autosomal recessive spinalmuscular atrophy.[J]Lancet,1995,345:985-986.
272.杨晓苏,张丽芳,肖波.SMN蛋白与脊髓性肌萎缩症的研究进展[J].中国神经科学杂志,2002,18(4):740-743.
273.Manzur A,Muntoni F,Simonds A.Muscular Dystrophy Campaign sponsord workshop:Recommendation for Respiratory Care of Children with Spinal Muscular Atrophy Type Ⅱ and Ⅲ[J].Neuro muscul Disord,2003,13(2):184-189.
274.JonesMA,McEwen IR,Hansen L.Use of power mobility for a young child with spinal muscular atrophy[J].PhysTher,2003,83(3):253-262.
275.Richardson RM,F illmoer HL,H olloway KL,et al.Progress in cerebraltran splantation of expanded neuronal stem cells[J].Neurosurg.,2004.100(4):659-671.
276.Eriksson C,B jorklund A,W ictorin K.Neuronal diferentiation following transplantation of e xpanded mouse neuro sphere cultures derived from different embry of brain regions[J].Exp Nenrol.2003 184(2):615-35.
277.Alvarez-BuyllaA,L im DA,et al.For the long run:maintaining germinal niches in the adult brain.[J]Nenron.2004.41(5):683-685.
278.Liang P,ZhaoS,K awamotoK,et al.Neuronal and glial diferentiation following of the human embryonic cortical stem cells[J].Hum Cell.2003.16(3):151-156.
279.ZhangS C.Embryonic stem cells for neural replacement therapy:prospects and chalenges.[J]Hematother Stem Cell Res.2003.12(6):625-634.
280.RipponH I,B ishopA E.Embryonic stem ce lls[J].Cell Prolif,.2004.37(1):23-34.
281.Lopez M oratalla N,Gonzalezd,et alI.Human plulrpotent stems cells[J].Rev Med Univ Navarra.,2003,47(3):34-42.
282.诸敏.邓志锋,汪决.干细胞移植在中枢神经系统疾病疗中的进展[J].国际脑血管病杂志,2007,15(3):224-226.
283.王金花.干细胞在脊髓损伤治疗中的研究进展[J].临床研究,2007,14(17):52-55.
284.张延洁,蔡芳,杨一峰.中老年人骨髓间质干细胞的生物学特性研究[J].中国生物工程杂志,2005,25(4):33-37.
285.雷俊霞,郭振宇,赵东长.大鼠骨髓间质干细胞对同种骨髓移植后造血重建和免疫重建的作用[J].中国病理生理杂志,2006,22(6):1129-1132.
286.Le Blanc K,Rasmusson I,Gotherstrom C,et al.Mesenchymal stem cells inhibit the expression of CD25(interleukin-2 receptor)and CD38 on phytohaemagglutinin-activated lymphocytes[J].Scand J Immunol,2004,60(3):307-315.
287.Frank MH,Sayegh MH.Immunomodulatory functions of mesenchymal stem ceils[J].Lancet,2004,363(9419):1411-1412.
288.王灯亮,康德智,林建华.间质干细胞修复脊髓损伤的理论与应用[J].中国临床康复,2006,10(17):155-157.
289.CHOPP M,ZHANGX H,LI Y,et al.Spinal cord injury in rat:treatment with bone marrow stromal cell transplantation[J].Neuoreport,2000,11(13):3001.
290.Li CH,Jiao BH.Effect of bone marrow stromal cells transfected with interleukin 18 on growth of intracranial glioma in rats[J].Ai Zheng,2007,26(1):38-43.
291.Chert J,LiY,Katakowski M,et al.Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat[J]..Neurosci Res,,2003,73:778-786.
292.张珊红,郭亮,龚卫琴.骨髓间充质干细胞在脑缺血再灌注大鼠脑内存活并分化为神经元样细胞的实验研究[J].中国老年学杂志,2007,27(19):1871-1873.
293.王颖,邓宇斌,李艳.骨髓间质干细胞诱导为神经元样细胞移植治疗脑梗塞模型大鼠后运动功能的改善[J].中国病理生理杂志,2006,22(12):2401-2406.
294.刘晓刚,邓宇斌.神经元样细胞与控释神经营养因子联合移植对脊髓损伤猴后索结构修复和功能恢复的作用[J].中国临床康复,9(26):96-99.
295.程伟,邬伟,郑国庆.中药诱导干细胞分化为神经元样细胞的研究思路和方法[J].中华中医药学刊,2007,25(7):1403-1405.
296.杨晓苏,吴海香,肖波.人骨髓间质干细胞分化为神经元样细胞及运动神经元生存蛋白表达的研究[J].中华医学杂志,85(16):1125-1127.
297.罗新明,杨晓苏,肖波.丙戊酸对脊髓性肌萎缩症患者神经元样细胞SMN2基因mRNA表达的影响[J].中华神经科杂志,2006,69(3):159-161.
298.Lou S,Gu P,Chen F,et al.The effect of bone marrow stromal cells on neuronal differentiation of mesencephalic neural stem cells in Sprague-Dawley rats[J].Brain Res,2003,968(1):114-121.
299.王斌,罗毅文,胡年宏.骨康方含药血清诱导大鼠骨髓基质细胞向成骨细胞方向分化的实验研究[J].中国中医骨伤科杂志,2007,15(11):31-33.
300.杨丽.OP模鼠MSCs生物学特性及药物对大鼠MSCs骨向分化的干预研究.暨南大学2007年博士学位论文,101-102.
301.史丙俊.中药血清药理学研究初探[J].泸州医学院学报,2003,26(3):272-273.
302.#12
303.冯瑜娟,邓翀,孟宪丽.血清药物化学应用于中药复方配伍研究的思路[J].中药药理与临床2007,23(5):261-263.
304.齐炼文,李萍,赵静.代谢组学与中医药现代研究[J].世界科学技术-中医药现代化,2006,(06):177-179.
305.黄敏,杨坤杰.中药血清药理学的研究方法及其改进思路[J].湖北中医杂志,2006,28(5):24-26.
306.米永杰,李健.中药血清药理学研究概述[J].四川解剖学杂志,2006,14(4):34-36.
307.王睿冲药血清药理学研究进展[J].齐齐哈尔医学院学报,2006,27(18):2243-2244.
308.罗焕敏.“血清药理学”与“血浆药理学”[J].中国药理学通报,2003,19(9):1075-1076.
309.陆慧晶.血清药理学研究中的若干问题探讨[J].基层中药杂志,2000,14(3):51.
310.崔玲,晏卫东.中药血清药理研究方法回顾与展望[J].武警医学,2004,15(6):460-461.
311.李仪奎.中药血清药理学实验方法的若干问题[J].中药新药与临床药理,1999,10(2):95-96.
312.王宁生,杨奎,雷燕,等.关于血清药理学的若干思考[J].中国中西医结合杂志,1999,19(5):263.
313.王力倩,李仪奎.血清药理学方法研究探索[J].中药药理与临床,1997,13(3):29-31.
314.梁春敏,王贤喜,董群,等.玉屏风散对小鼠免疫调节作用的血清药理学研究[J].上海免疫学杂志,2003,23(6):285-287.
315.赵明奇,吴伟康,段新芬,等.四逆汤对阿霉素心衰性大鼠线粒体功能的影响[J].中药材,2005,28(6):486-488.
316.Yang YF,Wang YQ.Study on standardization of serum pharmacologic study on Chinese composite recipes[J].Chin J Integrated Tradit West Med,2000;20(5):380-381.
317.杨仙凌.中药血清药理学研究方法应用现状及展望[J].浙江中医药大学学报,2007,31(5):662-665.
318.熊鹰.孔小云,陈如山,等.复方犀黄丸含药血清体外抗癌作用的研究[J].中国中西医结合消化杂志,2001;9(4):82.
319.吴健宇,穆静,李仪奎,等.血清药理学方法体外实验系统的血清添加量问题[J].上海中医药杂志,2001;(9):8-11.
320.王睿.药血清药理学研究进展[J].齐哈尔医学院学报,2006,27(18):2243-2246.
321.孟凡刚,吴承远.丹参诱导成年小鼠骨髓基质干细胞分化为神经细胞研究[J].上海中医药杂志,2005,39(10):48-49.
322.杨立业,刘湘名,惠国桢,等.丹参诱导鼠骨髓间充质细胞向神经元分化[J].中华儿科杂志,2003,41(8):607-610.
323.李爱秀.中药“药效团药性假说”的提出[J].天津药学,2007,19(2):42-44.
324.王喜军,张宁,常存宽.方剂配伍规律的研究现状和未来发展[J].世界科学技术-中医药现代化,2006,8(4):13-15.
325.黄敏,杨坤杰.中药血清药理学的研究方法及其改进思路[J].湖北中医杂志,2006,28(5):24-26.
326.李萍,刘惠如,黄涛.中药血清药物化学对中医现代化的意义[J].传统医药,2007,16,(2):58.