菌床养猪中主要细菌的分离鉴定
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摘要
本课题从四川川安有限公司的菌床养猪场的养殖垫料中分离筛选出6株细菌,分别定名为X1、X2、X3、X4、X5、X6。先通过外观和实验对其形态学和理化等特征进行初步鉴定,再通过PCR测序进行分子鉴定,判定其种属。
经鉴定,X1为沙雷氏菌属(Serratia);X2为假单胞菌属(Pseudomonas); X3为节杆菌属(Arthrobacter);X4为绿脓杆菌(Pseudomonas aeruginosa) ;X5为芽孢杆菌属(Bacillus);X6为埃希氏菌属(Escherichia)。
通过分子鉴定,可以判定到种的是:X1为粘质沙雷氏菌(Serratia marcescens);X3为黄色节杆菌(Arthrobacter flavescens);X4为绿脓杆菌(Pseudomonas aeruginosa);X5为枯草芽孢杆菌(Bacillus subtilis);X6为大肠埃希氏菌(Escherichia coli)。
The study isolated and selected 6 bacteria strains from the deep-litter of the Scale pig farm with deep-litter-system of Chuan’an LTD in Chengdu.SC. They were named X1、X2、X3、X4、X5、X6. According to the research on features of colony form, individual form and the determination of its physiological and biochemical character, the identification was finally determined by the PCR Sequencing.
The results proved that X1 was Serratia; X2 was Pseudomonas; X3 was Arthrobacter; X4 was Pseudomonas aeruginosa; X5 was Bacillus; X6 was Escherichia.
By identifing their molecular formation , and then we can sort out the bateria strains and put them into their species: X1 - Serratia marcescens; X3- Arthrobacter flavescens; X4 - Pseudomonas aeruginosa; X5 Bacillus subtilis -; X6 - Escherichia coli.
经鉴定,X1为沙雷氏菌属(Serratia);X2为假单胞菌属(Pseudomonas); X3为节杆菌属(Arthrobacter);X4为绿脓杆菌(Pseudomonas aeruginosa) ;X5为芽孢杆菌属(Bacillus);X6为埃希氏菌属(Escherichia)。
通过分子鉴定,可以判定到种的是:X1为粘质沙雷氏菌(Serratia marcescens);X3为黄色节杆菌(Arthrobacter flavescens);X4为绿脓杆菌(Pseudomonas aeruginosa);X5为枯草芽孢杆菌(Bacillus subtilis);X6为大肠埃希氏菌(Escherichia coli)。
The study isolated and selected 6 bacteria strains from the deep-litter of the Scale pig farm with deep-litter-system of Chuan’an LTD in Chengdu.SC. They were named X1、X2、X3、X4、X5、X6. According to the research on features of colony form, individual form and the determination of its physiological and biochemical character, the identification was finally determined by the PCR Sequencing.
The results proved that X1 was Serratia; X2 was Pseudomonas; X3 was Arthrobacter; X4 was Pseudomonas aeruginosa; X5 was Bacillus; X6 was Escherichia.
By identifing their molecular formation , and then we can sort out the bateria strains and put them into their species: X1 - Serratia marcescens; X3- Arthrobacter flavescens; X4 - Pseudomonas aeruginosa; X5 Bacillus subtilis -; X6 - Escherichia coli.
引文
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