海藻酸钠—明胶共混体系为载体的可注射式组织工程骨的研究
|
摘要
目的:探讨海藻酸钠—明胶共混体系为载体的可注射式组织工程骨的理化性能、生物相容性及其在临床上的应用前景。
方法:从兔骨髓中分离骨髓间充质干细胞,进行体外培养、分离和扩增,诱导向成骨细胞分化并进行检测和观察。探讨了不同浓度的氯化钙溶液对成骨细胞的影响作用。研究不同配比共混体系的理化性能和不同消毒方法对其的影响作用和成骨细胞在体系中的生物学行为。在从细胞遗传学角度考察了共混体系安全性的基础上,进行动物实验,观察材料的组织相容性和不同时间段新骨的形成。
结果:骨髓间充质干细胞经诱导培养后分化为成骨细胞,Ca~(2+)浓度对成骨细胞的代谢活性有影响作用。共混体系理化性能随不同配比的浓度、温度有所变化。各种消毒方法对其理化性能均有影响,其中,以高压蒸汽法对海藻酸钠粉末的影响最小。成骨细胞在共混体系中,呈悬浮状生长并增殖。植入共混体系/成骨细胞凝胶的动物,未见到有染色体型畸变。凝胶注射植入实验兔皮下后有软骨样组织和骨组织形成,并以软骨化骨的形式修复了实验动物的颅骨极限缺损。
结论:骨髓间充质干细胞可通过体外诱导培养分化为成骨细胞。不同浓度和作用时间的氯化钙对成骨细胞具有影响作用。海藻酸钠—明胶共混体系的理化性能可满足作为成骨细胞载体的需要。高压蒸汽对海藻酸钠粉末进行消毒的方法对共混体系的理化性能影响最小。共混体系无明显致染色体型畸变作用。通过注射方式在动物软组织中有异位成骨作用。可以用来进行骨缺损的修复。
Object To explore physico-chemical property,biocompatibility and clinical application of injectable tissue engineering bone carried by algin-gelatin complex.
Methods Bone marrow mesenchymal stem cells were abstracted from rabbit bone marrow.And then culture,isolation and amplification were performed in vitro. Osteoblasts,drived from Bone marrow mesenchymal stem cells,were measured and observed.Effect of different concentration solution of calcium chloride on osteoblasts was studied.Effect of different matching ratio complex and sterilization methods on complex was investigated.From cytogenetics,safety,and biocompatibility,of complex, and duration of osteogenesis were explored.
Results Bone marrow mesenchymal stem cells were induced into osteoblasts. Concentration of Ca~(2+) could influence metabolic activity of osteoblasts.Different matching ratio,temperature and sterilization methods could change physico-chemicai property of complex.Vapor sterilization for powder of algin had little effect on physico-chemical property of complex.Osteoblasts in complex system grew and proliferated in suspension.To the animals,which were transplanted in complex system of gels and cells,aberration of chromosomal pattern did not occur.After transplantation of the gel by injected into subcutaneous tissue,chondrogenesis and osteogenesis happened.And maximum defect of skull was repaired with os cartilaginea.
Conclusion Bone marrow mesenchymal stem cells can be induced into osteoblasts in vitro.Different concentration and duration of calcium chloride have effects on osteoblasts.Physico-chemical property of algin-gelatin complex can satisfy requirement of osteoblasts cartier.Vapor sterilization for powder of algin has little effect on physico-chemical property of complex.Complex system has no effect of chromosomal pattern aberration.Using the injectable method heterotopic bone can be applied to repair defect of bone.
方法:从兔骨髓中分离骨髓间充质干细胞,进行体外培养、分离和扩增,诱导向成骨细胞分化并进行检测和观察。探讨了不同浓度的氯化钙溶液对成骨细胞的影响作用。研究不同配比共混体系的理化性能和不同消毒方法对其的影响作用和成骨细胞在体系中的生物学行为。在从细胞遗传学角度考察了共混体系安全性的基础上,进行动物实验,观察材料的组织相容性和不同时间段新骨的形成。
结果:骨髓间充质干细胞经诱导培养后分化为成骨细胞,Ca~(2+)浓度对成骨细胞的代谢活性有影响作用。共混体系理化性能随不同配比的浓度、温度有所变化。各种消毒方法对其理化性能均有影响,其中,以高压蒸汽法对海藻酸钠粉末的影响最小。成骨细胞在共混体系中,呈悬浮状生长并增殖。植入共混体系/成骨细胞凝胶的动物,未见到有染色体型畸变。凝胶注射植入实验兔皮下后有软骨样组织和骨组织形成,并以软骨化骨的形式修复了实验动物的颅骨极限缺损。
结论:骨髓间充质干细胞可通过体外诱导培养分化为成骨细胞。不同浓度和作用时间的氯化钙对成骨细胞具有影响作用。海藻酸钠—明胶共混体系的理化性能可满足作为成骨细胞载体的需要。高压蒸汽对海藻酸钠粉末进行消毒的方法对共混体系的理化性能影响最小。共混体系无明显致染色体型畸变作用。通过注射方式在动物软组织中有异位成骨作用。可以用来进行骨缺损的修复。
Object To explore physico-chemical property,biocompatibility and clinical application of injectable tissue engineering bone carried by algin-gelatin complex.
Methods Bone marrow mesenchymal stem cells were abstracted from rabbit bone marrow.And then culture,isolation and amplification were performed in vitro. Osteoblasts,drived from Bone marrow mesenchymal stem cells,were measured and observed.Effect of different concentration solution of calcium chloride on osteoblasts was studied.Effect of different matching ratio complex and sterilization methods on complex was investigated.From cytogenetics,safety,and biocompatibility,of complex, and duration of osteogenesis were explored.
Results Bone marrow mesenchymal stem cells were induced into osteoblasts. Concentration of Ca~(2+) could influence metabolic activity of osteoblasts.Different matching ratio,temperature and sterilization methods could change physico-chemicai property of complex.Vapor sterilization for powder of algin had little effect on physico-chemical property of complex.Osteoblasts in complex system grew and proliferated in suspension.To the animals,which were transplanted in complex system of gels and cells,aberration of chromosomal pattern did not occur.After transplantation of the gel by injected into subcutaneous tissue,chondrogenesis and osteogenesis happened.And maximum defect of skull was repaired with os cartilaginea.
Conclusion Bone marrow mesenchymal stem cells can be induced into osteoblasts in vitro.Different concentration and duration of calcium chloride have effects on osteoblasts.Physico-chemical property of algin-gelatin complex can satisfy requirement of osteoblasts cartier.Vapor sterilization for powder of algin has little effect on physico-chemical property of complex.Complex system has no effect of chromosomal pattern aberration.Using the injectable method heterotopic bone can be applied to repair defect of bone.
引文
1.司徒镇强,吴军正 主编.细胞培养.西安.世界图书出版公司,1996.
2.王慧明,王模堂,王翰章,等.人胚成骨细胞体外分离及连续传代培养的初步研究.华西口腔医学杂志,1994,12(03):216-219.
3.Cooper LF,Masuda T,Whitson SW,et,al.Formation of mineralizing osteoblast cultures on machined,titanium oxide grit-blasted,and plasma-sprayed titanium surfaces,lnt J Oral Maxillofac Implants.1999,14(1):37-47.
4.Malaval L,Liu F,Roche P,et,al.Kinetics of osteoprogenitor proliferation and osteoblast differentiation in vitro.J Cell Biochem.1999,74(4):616-27.
5.Riccio V,Della Ragione F,Marrone G,et,al.Cultures of human embryonic osteoblasts.A new in vitro model for biocompatibility studies.Clin Orthop Relat Res.1994,(308):73-8.
6.Hankey DP,McCabe RE,Doherty MJ,Enhancement of human osteoblast proliferation and phenotypic expression when cultured in human serum.Acta Orthop Scand.2001,72(4):395-403.
7.Malekzadeh R,Hollinger JO,Buck D,et,al.Isolation of human osteoblast-like cells and in vitro amplification for tissue engineering.J Periodontol.1998,69(11):1256-62.
8.陈富林,毛天球,杨维东,等.培养成骨细胞接种于天然珊瑚中再造骨组织的研究.实用口腔医学杂志,1999,15(05):386-387.
9.毛天球,陈富林,杨维东,等.骨组织工程的研究进展.现代康复,2001,(16):5-7.
10.Uchida S,Doi Y,Kudo H,et,al.Transient expression of activin betaA mRNA on osteoprogenitor cells in rat bone regeneration after drill-hole injury.Bone.2000,27(1):81-90.
11.Vacanti CA,Upton J.Tissue-engineered morphogenesis of cartilage and bone by means of cell transplantation using synthetic biodegradable polymer matrices.Clin Plast Surg.1994,21(3):445-62.
12.Breitbart AS,Grande DA,Kessler R,et,al.Tissue engineered bone repair of calvarial defects using cultured periosteal cells.Plast Reconstr Surg.1998,101(3):567-74;discussion 575-6.
13.Beresford JN.Osteogenic stem cells and the stromal system of bone and marrow.Clin Orthop Relat Res.1989,(240):270-80.
14.Oreffo RO,Triffitt JT.Future potentials for using osteogenic stem cells and biomaterials in orthopedics.Bone.1999,25(2 Suppl):5S-gS.
15.Kadiyala S,Young RG,rhiede MA,et,al.Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro.Cell Transplant.1997,6(2):125-34.
16.Bruder SP,Jaiswal N,Haynesworth SE.Growth kinetics,self-renewal,and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation.J Cell Biochem.1997,64(2):278-94.
17.Nuttall ME,Patton AJ,Olivera DL,et,al.Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype:implications for osteopenic disorders.J Bone Miner Res.1998,13(3):371-82.
18.Jaiswal N,Haynesworth SE,Caplan AI,et,al.Osteogenic differentiation of purified,culture-expanded human mesenchymal stem cells in vitro.J Cell Biochem.1997,64(2):295-312.
19.Digirolamo CM,Stokes D,Colter D,et,al.Propagation and senescence of human marrow stromal cells in culture:a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate.Br J Haematol.1999,107(2):275-81.
20.Maniatopoulos C,Sodek J,Melcher AH.Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats.Cell Tissue Res.1988,254(2):317-30.
21.刘延青,娄思权.骨髓基质中的骨源性干细胞.中华骨科杂志,2000,20(2):49-51.
22.陈富林 毛天球,马秦,等.骨髓细胞体外成骨能力的实验研究.华西口腔医学杂志,1998,16(03):15-16.
23.Lane JM,Yasko AW,Tomin E,et,al.Bone marrow and recombinant human bone morphogenetic protein-2 in osseous repair.Clin Orthop Relat Res.1999 Apr;(361):216-27.
24.Caplan AI,Elyaderani M,Mochizuki Y,et,al.Principles of cartilage repair and regeneration.Clin Orthop Relat Res.1997,(342):254-69.
25.Bruder SP,Fox BS.Tissue engineering of bone.Cell based strategies.Clin Orthop Relat Res.1999,(367 Suppl):S68-83.
26.邱丽燕,王金福.骨髓间充质干细胞的研究进展,生物工程学报,2003,19(02):136-40.
27.Mueller SM,Glowacki J.Age-related decline in the osteogenic potential of human bone marrow cells cultured in three-dimensional collagen sponges.J Cell Biochem.2001,82(4):583-90.
28.Hughes SS,Hicks DG,O'Keefe RJ,et,al.Shared phenotypic expression of osteoblasts and chondrocytes in fracture callus.J Bone Miner Res.1995,10(4):533-44.
29.Fleming JE Jr,Cornell CN,Muschler GF.Bone cells and matrices in orthopedic tissue engineering.Orthop Clin North Am.2000,31(3):357-74.
30.邓廉夫,汤雪明,柴本甫,等.肿瘤坏死因子-α和骨形成发生蚩白-2诱导成纤维细胞成骨表型表达的体外研究.中国修复重建外科杂志,1998,12(04):49-53.
31.胡显文,陈昭烈,黄培堂.人胚胎干细胞的研究.生物技术通讯,2000,11(02):135-140.
32.Thomson JA,Itskovitz-Eldor J,et,al.Embryonic stem cell lines derived from human blastocysts.Science.1998,282(5391):1145-7.
33.Schuldiner M,Yanuka O,Itskovitz-Eldor J,et,al.Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells.Proc Natl Acad Sci U S A.2000,97(21):11307-12.
34.中国国家人类基因组南方研究中心伦理委员会.人类胚胎干细胞研究的伦理准则(建议稿).医学与哲学,2003,24(02):19-21.
35.Abdallah BM,Haack-Sφrensen M,Burns JS,et,al.Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene despite [corrected]extensive proliferation.Biochem Biophys Res Commun.2005,326(3):527-38.
36.Cheng SL,Lou J,Wright NM,et,al.In vitro and in vivo induction of bone formation using a recombinant adenoviral vector carrying the human BMP-2 gene.Calcif Tissue Int.2001,68(2):87-94
37.戴克戎,徐小良,汤亭亭,等.骨形态发生蛋白-2基因修饰的组织工程化骨修复羊胫骨干骨缺损.中华医学杂志,2003,83(15):55-60.
38.Blum JS,Barry MA,Mikos AG,et,al.In vivo evaluation of gene therapy vectors in ex vivo-derived marrow stromal cells for bone regeneration in a rat critical-size calvarial defect model.Hum Gene Ther.2003,14(18):1689-701.
39.Moutsatsos IK,Turgeman G,Zhou S,et,al.Exogenously regulated stem cell-mediated gene therapy for bone regeneration.Mol Ther.2001,3(4):449-61.
40.Gazit D,Turgeman G,Kelley P,et,al.Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone:a novel cell-mediated gene therapy.J Gene Med.1999,1(2):121-33.
41.郑启新,郭晓东,刘勇,等.bFGF基因修饰的间充质干细胞/多孔β-TCP陶瓷复合移植修复兔节段性骨缺损.中国生物医学工程学报,2003,22(2):171-177.
42.Zuk PA,Zhu M,Mizuno H,et,al.Multilineage ceils from human adipose tissue:implications for cell-based therapies.Tissue Eng.2001,7(2):211-28.
43.Zuk PA,Zhu M,Ashjian P,et,al.Human adipose tissue is a source of multipotent stem cells.Mol giol Cell.2002,13(12):4279-95.
44.Mizuno H,Hyakusoku H.Nesengenic potential and future clinical perspective of human processed lipoaspirate cells.J Nippon Med Sch.2003,70(4):300-6.
45.Oragoo JL,Choi JY,Lieberman JR,et,al.Bone induction by BMP-2transduced stem cells derived from human fat.J Orthop Res.2003,21(4):622-9.
46.Dragoo JL,Samimi B,Zhu M,et,al.Tissue-engineered.cartilage and bone using stem cells from human infrapatellar fat pads.J Bone Joint Surg Br.2003,85(5):740-7.
47.Rickard DJ,Sullivan TA,Shenker BJ,et,al.Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2.Dev Biol.1994,161(1):218-28.
48.颜晓慧,郑磊,洪华容,等.骨组织工程应用可降解聚合物的可能性探讨.国外医学—生物学工程分册,2000,23(01):11-17.
49.杨维东.口腔颌面骨替代材料与骨组织工程.实用口腔医学杂志,1999,15(6):469-471.
50.许永华,施新猷,胡蕴玉,等.同种异体成骨细胞-煅烧骨复合物动物体内植入.第四军医大学学报,2002,23(03):223-226.
51.刘玮,胡蕴玉,陆裕朴,等.重组合异种骨的研制及其生物活性分析.第四军医大学学报,,1992,13(01):11.
52.Urist MR.Bone:formation by autoinduction.Science.1965 Nov 12;150(698):893-9.
53.杨维东,彭品祥,马振国,等.异体脱矿骨修复创伤性眶底缺损,实用口腔医学杂志,1994,10(3):175-177.
54.马振国,周树夏,刘宝林.部分脱矿异体骨移植修复大块下颌骨缺损的实验研究.实用口腔医学杂志,1994,10(3):155-157.
55.Aspenberg P,Lohmander LS,Thorngren KG.Failure of bone induction by bone matrix in adult monkeys.J Bone Joint Surg Br.1988,70(4):625-7.
56.Thoren K,Aspenberg P,Thorngren KG.Lipid extraction decreases the specific immunologic response to bone allografts in rabbits.Acta Orthop Scand.1993,64(1):44-6.
51.Urist MR,Iwata H,Ceccotti PL,et,al.Bone morphogenesis in implants of insoluble bone gelatin.Proc Natl Acad Sci U S A.1973,70(12):3511-5.
58.Kirker-Head CA,Gerhart TN,Schelling SH,et,al.Long-term healing.of bone using recombinant human bone morphogenetic protein 2.Clin Orthop Relat Res.1995,(318):222-30.
59.Takagi K,Urist MR.The reaction of the dura to bone morphogenetic protein(BMP) in repair of skull defects.Ann Surg.1982,196(1):100-9.
60.Kakiuchi M,Hosoya T,Takaoka K,et,al.Human bone matrix gelatin as a clinical alloimplant.A retrospective review of 160 cases.Int Orthop.1985,9(3):181-8.
61.王勤涛 史俊南 吴织芬.牙周复合膜材料的生物学评价.实用口腔医学杂志,1996,12(02):36-38.
62.Sasano Y,Mizoguchi I,Takahashi I,et,al.BMPs induce endochondral ossification in rats when implanted ectopically within a carrier made of fibrous glass membrane.Anat Rec.1997,247(4):472-8.
63.Takaoka K,Nakahara H,Yoshikawa H,et,al.Ectopic bone induction on and in porous hydroxyapatite combined with collagen and bone morphogenetic protein.Clin Orthop Relat Res.I988,(234):250-4.
64.Itoh S,Kikuchi M,Takakuda K,et,al.The biocompatibility and osteoconductive activity of a novel hydroxyapatite/collagen composite biomaterial,and its function as a carrier of rhBMP-2.J Biomed Mater Res.2001,54(3):445-53.
65.Hendrickson DA,Nixon AJ,Erb HN,et,al.Phenotype and biological activity of neonatal equine chondrocytes cultured in a three-dimensional fibrin matrix.Am J Vet Res.1994,55(3):410-4.
66.Kawamura M,Iwata H,Sato K,et,al.Chondroosteogenetic response to crude bone matrix proteins bound to hydroxyapatite.Clin Orthop Relat Res.1987,(217):281-92..
67.Omura S,Mizuki N,Kawabe R,A carrier for clinical use of recombinant human BMP-2:dehydrothermally cross-linked composite of fibrillar and denatured atelocollagen sponge.Int J Oral Maxillofac Surg.1998,27(2):129-34.
68.Langer R.Biomaterials in drug delivery and tissue engineering:one laboratory's experience,Acc Chem Res.2000,33(2):94-101
69.Ruel-Gariepy E,Chenite A,Chaput C,et,al.Characterization of thermosensitive chitosan gels for the sustained delivery of drugs.Int J Pharm.2000,10;203(1-2):89-98.
70.Chenite A,Chaput C,Wang D,et,al.Novel injectable neutral solutions of chitosan form biodegradable gels in situ.Biomaterials.2000,21(21):2155-61.
71.Molinaro G,Leroux JC,Damas J,et,al.Biocompatibility of thermosensitive chitosan-based hydrogels:an in vivo experimental approach to injectable biomaterials.Biomaterials.2002,23(13):2717-22.
72.Souyris F,Pellequer C,Payrot C,et,al.Coral,a new biomedical material.Experimental and first clinical investigations on Madreporaria.J Maxillofac Surg.1985,13(2):64-9.
73.郑有华,任材年,陈小华,等.复合珊瑚骨修复颌骨实验性骨缺损.临床口腔医学杂志,1996,12(03):143-145.
74.张森林,毛天球,王会信,等.重组人骨形成蛋白2-珊瑚复合人工骨修复骨质缺损的实验研究.中华口腔医学杂志,1998,33(01):13-14.
75.韩亮,毛天球,戴毅敏,等.珊瑚转化多孔羟基磷灰石复合rhBMP-2人工骨的动物实验研究.实用口腔医学杂志,1999,15(04):46-48.
76.Jarcho M.Calcium phosphate ceramics as hard tissue prosthetics.Clin Orthop Relat Res.1981,(157):259-78..
77.Renooij W,Hoogendoorn HA,Visser WJ,et,al.Bioresorption of ceramic strontium-85-1abeled calcium phosphate implants in dog femora.A pilot study to quantitate bioresorption of ceramic implants of hydroxyapatite and tricalcium orthophosphate in vivo.Clin Orthop Relat Res.1985,(197):272-85.
78.Hollinger JO,Battistone GC.Biodegradable bone repair materials.Synthetic polymers and ceramics.Clin Orthop Relat Res.1986,(207):290-305.
79.Levin MP,Getter L,Cutright DE,Bhaskar SN.Biodegradable ceramic in periodontal defects.Oral Surg Oral Med Oral Pathol.1974,38(3): 344-51.
80.王海亭,陈磊,金雪玲,等.羟基磷灰石(HAP)材料在生物材料中的研究与开发.山东陶瓷.2002,25(2):3-8.
81.王莉丽,赵康,陈永楠.凝胶成型法制备多孔羟基磷灰石生物陶瓷.硅酸盐通报,2005,24(2):110-112.
82.孙劲东,徐韵涛.SD大鼠骨髓基质细胞与多孔羟基磷灰石陶瓷支架体外粘附的实验研究.海南医学,2005,16(10):140-141.
83.Casabona F,Martin I,Muraglia A,et,al.Prefabricated engineered bone flaps:an experimental model of tissue reconstruction in plastic surgery.Plast Reconstr Surg.1998,101(3):577-81.
84.Kon E,Muraglia A,Corsi A,et,al.Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones.J Biomed Mater Res.2000,49(3):328-37.
85.郭昭庆,党耕町,王志国,等.种植骨髓基质细胞的骨组织工程学研究.中华外科杂志,1999,37(07):10-13.
86.Sayer M,Stratilatov AD,Reid J,et,al.Structure and composition of silicon stabilized tricalcium phosphate.Biomaterials.2003,24(3):369-82.
87.Reddi AH,Huggins CB.Influence of geometry of transplanted tooth and bone on transformation of fibroblasts.Proc Soc Exp Biol Med.1973,143(3):634-7.
88.Handschel J,Wiesmann HP,Stratmann U,et,al.TCP is hardly resorbed and not osteoconductive in a non-loading calvarial model.Biomaterials.2002,23(7):1689-95.
89.Pilliar RM,Filiaggi MJ,Wells JD,et,al.Porouscalcium polyphosphate scaffolds for bone substitute applications—in vitro characterization.Biomaterials.2001,22(9):963-72.
90.颜晓慧,洪华容,张晓.可降解聚合物用作骨组织工程细胞外基质材料的研究进展.北京生物医学工程,2000,19(02):123-128.
91.Vesala AL,Kallioinen M,T(o|¨)rm(a|¨)l(a|¨) P,et,al.Bone tissue engineering:treatment of cranial bone defects in rabbits using self-reinforced poly-L,D-lactide 96/4 sheets.J Craniofac Surg.2002,13(5):607-13.
92.Ishaug SL,Crane GM,Miller MJ,et,al.Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds.J Biomed Mater Res.1997,36(1):17-28.
93.Sikavitsas VI,Bancroft GN,Holtorf HL,et,al.Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces.Proc Natl Acad Sci U S A.2003,100(25):14683-8.
94.Agrawal CM,Best J,Heckman JD,et,al.Protein release kinetics of a biodegradable implant for fracture non-unions.Biomaterials.1995,16(16):1255-60.
95.Zellin G,Linde A.Importance of delivery systems for growth-stimulatory factors in combination with osteopromotive membranes.An experimental study using rhBMP-2 in rat mandibular defects.J Biomed Mater Res.1997,35(2):181-90.
96.Miyamoto S,Takaoka K,Okada T,et,al.Evaluation of polylactic acid homopolymers as carriers for bone morphogenetic protein Clin Orthop Relat Res.1992,(2?8):274-85..
97.Marra KG,Szem JW,Kumta PN,et,al.In vitro analysis of biodegradable polymer blend/hydroxyapatite composites for bone tissue engineering.J Biomed Mater Res.1999,47(3):324-35.
98.Saito N,Okada T,Horiuchi H,et,al.Biodegradable poly-D,L-lactic acid-polyethylene glycol block copolymers as a BMP delivery system for inducing bone.J Bone Joint Surg Am.2001,83-A Suppl 1(Pt 2):S92-8.
99.Oldham JB,Lu L,Zhu X,et,al.Biological activity of rhBMP-2 released from PLGA microspheres.J Biomech Eng.2000,122(3):289-92.
100.Murphy WL,Peters MC,Kohn DH,et,al.Sustained release of vascular endothelial growth factor from mineralized poly scaffolds for tissue engineering Biomaterials.2000,21(24):2521-7.
101.贺小兵,卢卫忠,唐康来,等.骨形成蛋白和转化生长因子-β对兔尺骨骨折愈合后生物力学性能的影响.中国修复重建外科杂志,2003,17(3):185-188.
102.Thomson RC,Yaszemski MJ,Powers JM,et,al.Hydroxyapatite fiber reinforced poly(alpha-hydroxy ester) foams for bone regeneration.Biomaterials.1998,19(21):1935-43.
103.Lin HR,Yeh YJ.Porous alginate/hydroxyapatite composite scaffolds for bone tissue engineering:preparation,characterization,and in vitro studies.J Biomed Mater Res B Appl Biomater.2004,71(1):52-65.
104.Peter SJ,Yaszemski MJ,Suggs LJ,et,al.Characterization of partially saturated poly(propylene fumarate) for orthopaedic application.J Biomater Sci Po]ym Ed.1997,8(11):893-904.
105.Mak K,Toriumi DM.Injectable filler materials for soft-tissue augmentation.Otolaryngol Clin North Am.1994 Feb;27(1):211-22.
106.Friess W.Collagen—biomaterial for drug delivery.Eur J Pharm Biopharm.1998 Mar;45(2):113-36.
107.Wu L,Zhang H,Zhang J,et,al.Fabrication of three-dimensional porous scaffolds of complicated shape for tissue engineering.Ⅰ.Compression molding based on flexible-rigid combined mold.Tissue Eng.2005 Jul-Aug;11(7-8):1105-14
108.Wu L,Jing D,Ding J.A "room-temperature" injection molding/particulate leaching approach for fabrication of biodegradable three-dimensional porous scaffolds.Biomaterials.2006 Jan;27(2):185-91.
109.段世锋,朱文,俞麟,等。一种新型可注射性组织工程水凝胶的双组分引发体系细胞毒性的负协同效应.科学通讯,2005,50(05):430-433.
110.Elisseeff J.Injectable cartilage tissue engineering.Expert Opin Biol Ther.2004 Dec;4(12):1849-59.
111.Hou,Q.P.;De Bank,P.A.;Shakesheff,K.M.,Injectable scaffolds for tissue regeneration.J.Mater.Chem.2004,14,1915-1923.
112.Basle MF,Grizon F,Pascaretti C,et,al.Shape and orientation of osteoblast-like cells(Saos-2) are influenced by collagen fibers in xenogenic bone biomaterial.J Biomed Mater Res.1998 Jun 5;40(3):350-7.
113.Shi S,Kirk M,Kahn AJ.The role of type Ⅰ collagen in the regulation of the osteoblast phenotype.J Bone Miner Res.1996Aug;11(8):1139-45.
114.Green J,Schotland S,Stauber DJ,et,al.Cell-matrix interaction in bone:type Ⅰ collagen modulates signal transduction in osteoblast-likecells.Am J Physiol.1995 May;268(5 Pt 1):C1090-103.
115.Roche S,Ronziere MC,Herbage D,et,al.Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering.Biomaterials.2001Jan;22(1):9-18.
116.Mizuno M,Shindo M,Kobayashi D,et,al.Osteogenesis by bone marrow stromal cells maintained on type Ⅰ collagen matrix gels in vivo.Bone.1997 Feb;20(2):101-7.
117.Wakitani S,Kimura T,Hirooka A,et,al.Repair of rabbit articular surfaces with allograft chondrocytes embedded in collagen gel.J Bone Joint Surg Br.1989 Jan;71(1):74-80.
118.Kang HW,Tabata Y,Ikada Y.Fabrication of porous gelatin scaffolds for tissue engineering.Biomaterials.1999 Jul;20(14):1339-44.
119.Rault I,Frei V,Herbage D,et,al.(1996) Evaluation of different chemical methods for cross-linking collagen gel,films and sponges.J Mater Sci Mater Med,7:215-221
120.Chevallay B,Abdul-Malak N,Herbage D.Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds:influence of crosslinking with diphenylphosphorylazide on matrix reorganization,growth,and biosynthetic and proteolyticactivities.J Biomed Mater Res.2000 Mar 15;49(4):448-59.
121.田卫东,乔鞠,李声伟,等.纤维蛋白粘合剂作为骨形成蛋白载体的实验研究.中国口腔种植学杂志,1999,4(1):17-20.
122.Perka C,Spitzer RS,Lindenhayn K,et,al.Matrix-mixed culture:new methodology for chondrocyte culture and preparation of cartilage transplants.J Biomed Mater Res.2000 Mar 5;49(3):305-11.
123.李征.纤维蛋白胶的作用原理和临床应用及不良反应.华西药学杂志,1999,14(1):39-41.
124.Homminga GN,Buma P,Koot HW,et,al.Chondrocyte behavior in fibrin glue in vitro.Acta Orthop Stand.1993 Aug;64(4):441-5.
125.Sims CD,Butler PE,Cao YL,et,al.Tissue engineered neocartilage using plasma derived polymer substrates and chondrocytes.Plast Reconstr Surg.1998 May;101(6):1580-5.
126.Hendrickson DA,Nixon AJ,Grande DA,et,al.Chondrocyte-fibrin matrix transplants for resurfacing extensive articular cartilage defects.J Orthop Ues.1994 Jul;12(4):485-97.
127.Ye Q,Z(u|¨)nd G,Benedikt P,et,al.Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering.Eur J Cardiothorac Surg.2000 May;17(5):587-91.
128.Ikari Y,Mulvihill E,Schwartz SM alpha 1-Proteinase inhibitor,alpha 1-antichymotrypsin,and alpha 2-macroglobulin are the antiapoptotic factors of vascular smooth muscle cells.J Biol Chem.2001 Apr 13;276(15):11798-803.
129.Meinhart J,Fussenegger M,H(o|¨)bling W.Stabilization of fibrin-chondrocyte constructs for cartilage reconstruction.Ann Plast Surg.1999 Jun;42(6):673-8.
130.池光范,候宜,候立中,等.胶原一纤维蛋白混合凝胶对关节软骨细胞合成分泌Ⅱ型胶原的组织学影响研究.第三届全国组织工程学术会议暨第一届中国国际组织工程会议论文集,成都,p 178-180
131.Peretti GM,Randolph MA,Zaporojan V,et,al.A biomechanical analysis of an engineered cell-scaffold implant for cartiiage repair.Ann Plast Surg.2001 May;46(5):533-7.
132.Draget KI,Skjak-Braek G,Smidsrφd O.Alginate based new materials.Int J Biol Macromol.1997 Aug;21(1-2):47-55.
133.Smidsrφd O,Skjak-Braek G.Alginate as immobilization matrix for cells.Trends Biotechnol.1990 Mar;8(3):71-8.
134.Kuo CK,Ma PX.Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering:part 1.Structure,gelation rate and mechanical properties.Biomaterials.2001 Mar;22(6):511-21.
I35.Majmudar G,Bole D,Goldstein SA,et,al.Bone cell culture in a three-dimensional polymer bead stabilizes the differentiated phenotype and provides evidence that osteoblastic ceils synthesize type Ⅲ collagen and fibronectin.J Bone Miner Res.1991Aug;6(8):869-81.
136.Hguselmann HJ,Fernandes RJ,Mok SS,et,al.Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads.J Cell Sci.1994 Jan;107(Pt 1):17-27.
137.Paige KT,Cima LG,Yaremchuk MJ,et,al.De novo cartilage generation using calcium alginate-chondrocyte constructs.Plast Reconstr Surg.1996 Jan;97(1):168-78.
138.Paige KT,Cima LG,Yaremchuk MJ,et,al.Injectable cartilage.Plast Reconstr Surg.1995 Nov;96(6):1390-8;discussion 1399-400.
139.Park DJ,Bong JP,Park SY,et,al.Cartilage generation using alginate-encapsulated autogenous chondrocytes in rabbits.Ann Otol Rhinol Laryngol.2000 Dec;109(12 Pt 1):1157-61.
140.Stevens MM,Qanadilo HF,Langer R,et,al.A rapid-curing alginate gel system:utility in periosteum-derived cartilage tissue engineering.Biomaterials.2004 Feb;25(5):887-94.
141.陶凯,毛天球,陈富林,等.骨髓基质成骨细胞-松质骨基质复合人工骨皮下移植成骨作用的实验研究.中华口腔医学杂志,2002 37(1):18-20.
142.Alsberg E,Anderson KW,Albeiruti A,et,al.Cell-interactive alginate hydrogels for bone tissue engineering.J Dent Res.2001Nov;80(11):2025-9.
143.孙磊,胡蕴玉,窦榆生,等.骨髓基质细胞在藻酸盐凝胶中的生物学行为.中国矫形外科杂志,2002,9(02):54-56.
144.Shang Q,Wang Z,Liu W,Shi Y,et,al.Tissue-engineered bone repair of sheep cranial defects with autologous bone marrow stromal cells.J Craniofac Surg.2001 Nov;12(6):586-93.
145.羊书勇,杨维东,雷德林,等.可注射组织工程骨的初步研究.实用口腔医学杂志,2001,17(04):271-273.
146.翁雨来,蔡霞,商庆新,等.骨髓基质干细胞-改良藻酸钙凝胶修复颅骨缺损的实验研究.上海口腔医学,2001,10(1):35-38.
147.Mi FL,Lin YM,Wu YB,et,al.Chitin/PLGA blend microspheres as a biodegradable drug-delivery system:phase-separation,degradation and release behavior.Biomaterials.2002 Aug;23(15):3257-67.
148.Klokkevold PR,Vandemark L,Kenney EB,et,al.Osteogenesis enhanced by chitosan(poly-N-acetyl glucosaminoglycan) in vitro.J Periodontol.1996 Nov;67(11):1170-5.
149.Madihally SV,Matthew HW.Porous chitosan scaffolds for tissue engineering.Biomaterials.1999 Jun;20(12):1133-42.
150.Suh JK,Matthew HW.Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering:a review.Biomaterials.2000 Dec;21(24):2589-98.
151.Boland T,Mironov V,Gutowska A,et,al.Cell and organ printing 2:fusion of cell aggregates in three-dimensional gels.Anat Rec A Discov Mol Cell Evol Biol.2003 Jun;272(2):497-502.
152.Vercruysse KP,Marecak DM,Marecek JF,et,al.Synthesis and in vitro degradation of new polyvalent hydrazide cross-linked hydrogels of hyaluronic acid.Bioconjug Chem.1997 Sep-Oct;8(5):686-94.
153.Girotto D,Urbani S,Brun P,et,al.Tissue-specific gene expression in chondrocytes grown on three-dimensional hyaluronic acid scaffolds.Biomaterials.2003 Aug;24(19):3265-75.
154.Duranti F,Salti G,Bovani B,et,al.Injectable hyaluronic acid gel for soft tissue augmentation.A clinical and histological study.Dermatol Surg.1998 Dec;24(12):1317-25.
155.Barbucci R,Lamponi S,Borzacchiello A,et,al.Hyaluronic acid hydrogel in the treatment of osteoarthritis.Biomaterials.2002Dec;23(23):4503-13.
156.Aigner J,Tegeler J,Hutzler P,et,al.Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester. J Biomed Mater Res. 1998 Nov;42(2):172-81.
157. Dillon GP, Yu X, Sridharan A, et, al. The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold. J Biomater Sci Polym Ed. 1998;9(10):1049-69.
158. Diduch DR, Jordan LC, Mierisch CM, et,al. Marrow stromal cells embedded in alginate for repair of osteochondral defects. Arthroscopy. 2000 Sep;16(6):571-7
159. Liang HF, Hong MH, Ho RM, et, al. Novel method using a temperature-sensitive polymer (methylcellulose) to thermally gel aqueous alginate as a pH-sensitive hydrogel. Biomacromolecules. 2004 Sep-Oct;5(5):1917-25.
160. Choi HS, Yamamoto K, Ooya T, et,al. Synthesis of poly-grafted dextrans and their and thermosensitive hydrogelation with cyclodextrins. Chemphyschem. 2005 Jun 13;6(6):1081-6.
1.Oreffo RO,Triffitt JT.Future potentials for using osteogenic stem cells and biomaterials in orthopedics.Bone.1999,25(2 Suppl):5S-9S.
2.Kadiyala S,Young RG,rhiede MA,et,al.Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro.Cell Transplant.1997,6(2):125-34.
3.刘延青,娄思权.骨髓基质中的骨源性干细胞.中华骨科杂志,2000,20(2):49-51.
1.刘国廉主编.细胞毒理学.第一版.北京军事医学科学出版社,2000,367-370.
2.陈治清主编.口腔材料学.第二版.北京人民卫生出版社,2000,35-42.
3.Fragonas E,Valente M,Pozzi-Mucelli M,et,al.Articu]ar cartilage repair in rabbits by using suspensions of allogenic chondrocytes in alginate.Biomaterials.2000,21(8):795-801.
4.Marijnissen WJ,van Osch GJ,Aigner J,et al.Tissue-engineered cartilage using Serially passaged articular chondrocytes.Chondrocytes in alginate,combined in vivo with a synthetic(E210) or biologic biodegradable carrier(DBM).Biomaterials.2000,21(6):571-80.
5.Kuo CK,Ma PX.Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering:part 1.Structure,gelation rate and mechanical properties.Biomaterials.2001,22(6):511-21.
1.赵谋明,李敏,孙哲浩,等.明胶与海藻酸钠的相互作用及其应用.食品与发酵工业,2000,26(03)10-14.
2.郑洪河,张虎成,夏志清,等.海藻酸钠溶液的粘度性质与流变学特征.河南师范大学学报(自然科学版),1997,25(02),55-59.
3.严金龙,孙汝东,柏云杉,聂福礼,王肇福.明胶的粘度行为和谱学特征.皮革化工,1999,16(06),1-3.
1.Paige KT,Cima LG,Yaremchuk MJ,et al.Injectable cartilage.Plast Reconstr Surg.1995,96(6):1390-8.
2.Shang Q,Wang Z,Liu W,et,al.Tissue-engineered bone repair of sheep cranial defects with autologous bone marrow stromal cells.J Craniofac Surg.2001,12(6):586-93.
3.施绮云,宁国伯.对藻酸钠消毒方法的研究.中国消毒学杂志,1998,15(02),28-31.
1.Perka C,Spitzer RS,Lindenhayn K,et,al.Matrix-mixed culture:new methodology for chondrocyte culture and preparation of cartilage transplants.J Biomed Mater Res.2000,49(3):305-11.
2.Wang L,Shelton RM,Cooper PR,et,al.Evaluationof sodium alginate for bone marrow cell tissue engineering.Biomaterials.2003 24(20):3475-81.
3.王敏,翁雨来,胡晓洁,等。组织工程技术修复犬牙槽骨缺损的实验研究。中华医学杂志.2003,83(15),55-60.
1.GB/T 16886.1-2001.医疗器械生物学评价第1部分:评价与试验
1.Gauthier O,Bouler JM,Weiss P,et al.Kinetic study of bone ingrowth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes.J Biomed Mater Res.1999,47(1):28-35.
2.Gauthier O,Boix D,Grimandi G,et al.A new injectable calcium phosphate biomaterial for immediate bone filling of extraction sockets:a preliminary study in dogs.J Periodontol.1999,70(4):375-83.
3.杨维东.口腔颌面骨替代材料与骨组织工程.实用口腔医学杂志,1999,15(06):469-471.
1.Schmitz JP,Hollinger JO.The critical size defect as an experimental model for craniomandibulofacial nonunions.Clin Orthop Relat Res.1986,(205):299-308.
2.Hollinger JO,Kleinschmidt JC.The critical size defect as an experimental model to test bone repair materials.J Craniofac Surg.1990,1(1):60-8.
2.王慧明,王模堂,王翰章,等.人胚成骨细胞体外分离及连续传代培养的初步研究.华西口腔医学杂志,1994,12(03):216-219.
3.Cooper LF,Masuda T,Whitson SW,et,al.Formation of mineralizing osteoblast cultures on machined,titanium oxide grit-blasted,and plasma-sprayed titanium surfaces,lnt J Oral Maxillofac Implants.1999,14(1):37-47.
4.Malaval L,Liu F,Roche P,et,al.Kinetics of osteoprogenitor proliferation and osteoblast differentiation in vitro.J Cell Biochem.1999,74(4):616-27.
5.Riccio V,Della Ragione F,Marrone G,et,al.Cultures of human embryonic osteoblasts.A new in vitro model for biocompatibility studies.Clin Orthop Relat Res.1994,(308):73-8.
6.Hankey DP,McCabe RE,Doherty MJ,Enhancement of human osteoblast proliferation and phenotypic expression when cultured in human serum.Acta Orthop Scand.2001,72(4):395-403.
7.Malekzadeh R,Hollinger JO,Buck D,et,al.Isolation of human osteoblast-like cells and in vitro amplification for tissue engineering.J Periodontol.1998,69(11):1256-62.
8.陈富林,毛天球,杨维东,等.培养成骨细胞接种于天然珊瑚中再造骨组织的研究.实用口腔医学杂志,1999,15(05):386-387.
9.毛天球,陈富林,杨维东,等.骨组织工程的研究进展.现代康复,2001,(16):5-7.
10.Uchida S,Doi Y,Kudo H,et,al.Transient expression of activin betaA mRNA on osteoprogenitor cells in rat bone regeneration after drill-hole injury.Bone.2000,27(1):81-90.
11.Vacanti CA,Upton J.Tissue-engineered morphogenesis of cartilage and bone by means of cell transplantation using synthetic biodegradable polymer matrices.Clin Plast Surg.1994,21(3):445-62.
12.Breitbart AS,Grande DA,Kessler R,et,al.Tissue engineered bone repair of calvarial defects using cultured periosteal cells.Plast Reconstr Surg.1998,101(3):567-74;discussion 575-6.
13.Beresford JN.Osteogenic stem cells and the stromal system of bone and marrow.Clin Orthop Relat Res.1989,(240):270-80.
14.Oreffo RO,Triffitt JT.Future potentials for using osteogenic stem cells and biomaterials in orthopedics.Bone.1999,25(2 Suppl):5S-gS.
15.Kadiyala S,Young RG,rhiede MA,et,al.Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro.Cell Transplant.1997,6(2):125-34.
16.Bruder SP,Jaiswal N,Haynesworth SE.Growth kinetics,self-renewal,and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation.J Cell Biochem.1997,64(2):278-94.
17.Nuttall ME,Patton AJ,Olivera DL,et,al.Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype:implications for osteopenic disorders.J Bone Miner Res.1998,13(3):371-82.
18.Jaiswal N,Haynesworth SE,Caplan AI,et,al.Osteogenic differentiation of purified,culture-expanded human mesenchymal stem cells in vitro.J Cell Biochem.1997,64(2):295-312.
19.Digirolamo CM,Stokes D,Colter D,et,al.Propagation and senescence of human marrow stromal cells in culture:a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate.Br J Haematol.1999,107(2):275-81.
20.Maniatopoulos C,Sodek J,Melcher AH.Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats.Cell Tissue Res.1988,254(2):317-30.
21.刘延青,娄思权.骨髓基质中的骨源性干细胞.中华骨科杂志,2000,20(2):49-51.
22.陈富林 毛天球,马秦,等.骨髓细胞体外成骨能力的实验研究.华西口腔医学杂志,1998,16(03):15-16.
23.Lane JM,Yasko AW,Tomin E,et,al.Bone marrow and recombinant human bone morphogenetic protein-2 in osseous repair.Clin Orthop Relat Res.1999 Apr;(361):216-27.
24.Caplan AI,Elyaderani M,Mochizuki Y,et,al.Principles of cartilage repair and regeneration.Clin Orthop Relat Res.1997,(342):254-69.
25.Bruder SP,Fox BS.Tissue engineering of bone.Cell based strategies.Clin Orthop Relat Res.1999,(367 Suppl):S68-83.
26.邱丽燕,王金福.骨髓间充质干细胞的研究进展,生物工程学报,2003,19(02):136-40.
27.Mueller SM,Glowacki J.Age-related decline in the osteogenic potential of human bone marrow cells cultured in three-dimensional collagen sponges.J Cell Biochem.2001,82(4):583-90.
28.Hughes SS,Hicks DG,O'Keefe RJ,et,al.Shared phenotypic expression of osteoblasts and chondrocytes in fracture callus.J Bone Miner Res.1995,10(4):533-44.
29.Fleming JE Jr,Cornell CN,Muschler GF.Bone cells and matrices in orthopedic tissue engineering.Orthop Clin North Am.2000,31(3):357-74.
30.邓廉夫,汤雪明,柴本甫,等.肿瘤坏死因子-α和骨形成发生蚩白-2诱导成纤维细胞成骨表型表达的体外研究.中国修复重建外科杂志,1998,12(04):49-53.
31.胡显文,陈昭烈,黄培堂.人胚胎干细胞的研究.生物技术通讯,2000,11(02):135-140.
32.Thomson JA,Itskovitz-Eldor J,et,al.Embryonic stem cell lines derived from human blastocysts.Science.1998,282(5391):1145-7.
33.Schuldiner M,Yanuka O,Itskovitz-Eldor J,et,al.Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells.Proc Natl Acad Sci U S A.2000,97(21):11307-12.
34.中国国家人类基因组南方研究中心伦理委员会.人类胚胎干细胞研究的伦理准则(建议稿).医学与哲学,2003,24(02):19-21.
35.Abdallah BM,Haack-Sφrensen M,Burns JS,et,al.Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene despite [corrected]extensive proliferation.Biochem Biophys Res Commun.2005,326(3):527-38.
36.Cheng SL,Lou J,Wright NM,et,al.In vitro and in vivo induction of bone formation using a recombinant adenoviral vector carrying the human BMP-2 gene.Calcif Tissue Int.2001,68(2):87-94
37.戴克戎,徐小良,汤亭亭,等.骨形态发生蛋白-2基因修饰的组织工程化骨修复羊胫骨干骨缺损.中华医学杂志,2003,83(15):55-60.
38.Blum JS,Barry MA,Mikos AG,et,al.In vivo evaluation of gene therapy vectors in ex vivo-derived marrow stromal cells for bone regeneration in a rat critical-size calvarial defect model.Hum Gene Ther.2003,14(18):1689-701.
39.Moutsatsos IK,Turgeman G,Zhou S,et,al.Exogenously regulated stem cell-mediated gene therapy for bone regeneration.Mol Ther.2001,3(4):449-61.
40.Gazit D,Turgeman G,Kelley P,et,al.Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone:a novel cell-mediated gene therapy.J Gene Med.1999,1(2):121-33.
41.郑启新,郭晓东,刘勇,等.bFGF基因修饰的间充质干细胞/多孔β-TCP陶瓷复合移植修复兔节段性骨缺损.中国生物医学工程学报,2003,22(2):171-177.
42.Zuk PA,Zhu M,Mizuno H,et,al.Multilineage ceils from human adipose tissue:implications for cell-based therapies.Tissue Eng.2001,7(2):211-28.
43.Zuk PA,Zhu M,Ashjian P,et,al.Human adipose tissue is a source of multipotent stem cells.Mol giol Cell.2002,13(12):4279-95.
44.Mizuno H,Hyakusoku H.Nesengenic potential and future clinical perspective of human processed lipoaspirate cells.J Nippon Med Sch.2003,70(4):300-6.
45.Oragoo JL,Choi JY,Lieberman JR,et,al.Bone induction by BMP-2transduced stem cells derived from human fat.J Orthop Res.2003,21(4):622-9.
46.Dragoo JL,Samimi B,Zhu M,et,al.Tissue-engineered.cartilage and bone using stem cells from human infrapatellar fat pads.J Bone Joint Surg Br.2003,85(5):740-7.
47.Rickard DJ,Sullivan TA,Shenker BJ,et,al.Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2.Dev Biol.1994,161(1):218-28.
48.颜晓慧,郑磊,洪华容,等.骨组织工程应用可降解聚合物的可能性探讨.国外医学—生物学工程分册,2000,23(01):11-17.
49.杨维东.口腔颌面骨替代材料与骨组织工程.实用口腔医学杂志,1999,15(6):469-471.
50.许永华,施新猷,胡蕴玉,等.同种异体成骨细胞-煅烧骨复合物动物体内植入.第四军医大学学报,2002,23(03):223-226.
51.刘玮,胡蕴玉,陆裕朴,等.重组合异种骨的研制及其生物活性分析.第四军医大学学报,,1992,13(01):11.
52.Urist MR.Bone:formation by autoinduction.Science.1965 Nov 12;150(698):893-9.
53.杨维东,彭品祥,马振国,等.异体脱矿骨修复创伤性眶底缺损,实用口腔医学杂志,1994,10(3):175-177.
54.马振国,周树夏,刘宝林.部分脱矿异体骨移植修复大块下颌骨缺损的实验研究.实用口腔医学杂志,1994,10(3):155-157.
55.Aspenberg P,Lohmander LS,Thorngren KG.Failure of bone induction by bone matrix in adult monkeys.J Bone Joint Surg Br.1988,70(4):625-7.
56.Thoren K,Aspenberg P,Thorngren KG.Lipid extraction decreases the specific immunologic response to bone allografts in rabbits.Acta Orthop Scand.1993,64(1):44-6.
51.Urist MR,Iwata H,Ceccotti PL,et,al.Bone morphogenesis in implants of insoluble bone gelatin.Proc Natl Acad Sci U S A.1973,70(12):3511-5.
58.Kirker-Head CA,Gerhart TN,Schelling SH,et,al.Long-term healing.of bone using recombinant human bone morphogenetic protein 2.Clin Orthop Relat Res.1995,(318):222-30.
59.Takagi K,Urist MR.The reaction of the dura to bone morphogenetic protein(BMP) in repair of skull defects.Ann Surg.1982,196(1):100-9.
60.Kakiuchi M,Hosoya T,Takaoka K,et,al.Human bone matrix gelatin as a clinical alloimplant.A retrospective review of 160 cases.Int Orthop.1985,9(3):181-8.
61.王勤涛 史俊南 吴织芬.牙周复合膜材料的生物学评价.实用口腔医学杂志,1996,12(02):36-38.
62.Sasano Y,Mizoguchi I,Takahashi I,et,al.BMPs induce endochondral ossification in rats when implanted ectopically within a carrier made of fibrous glass membrane.Anat Rec.1997,247(4):472-8.
63.Takaoka K,Nakahara H,Yoshikawa H,et,al.Ectopic bone induction on and in porous hydroxyapatite combined with collagen and bone morphogenetic protein.Clin Orthop Relat Res.I988,(234):250-4.
64.Itoh S,Kikuchi M,Takakuda K,et,al.The biocompatibility and osteoconductive activity of a novel hydroxyapatite/collagen composite biomaterial,and its function as a carrier of rhBMP-2.J Biomed Mater Res.2001,54(3):445-53.
65.Hendrickson DA,Nixon AJ,Erb HN,et,al.Phenotype and biological activity of neonatal equine chondrocytes cultured in a three-dimensional fibrin matrix.Am J Vet Res.1994,55(3):410-4.
66.Kawamura M,Iwata H,Sato K,et,al.Chondroosteogenetic response to crude bone matrix proteins bound to hydroxyapatite.Clin Orthop Relat Res.1987,(217):281-92..
67.Omura S,Mizuki N,Kawabe R,A carrier for clinical use of recombinant human BMP-2:dehydrothermally cross-linked composite of fibrillar and denatured atelocollagen sponge.Int J Oral Maxillofac Surg.1998,27(2):129-34.
68.Langer R.Biomaterials in drug delivery and tissue engineering:one laboratory's experience,Acc Chem Res.2000,33(2):94-101
69.Ruel-Gariepy E,Chenite A,Chaput C,et,al.Characterization of thermosensitive chitosan gels for the sustained delivery of drugs.Int J Pharm.2000,10;203(1-2):89-98.
70.Chenite A,Chaput C,Wang D,et,al.Novel injectable neutral solutions of chitosan form biodegradable gels in situ.Biomaterials.2000,21(21):2155-61.
71.Molinaro G,Leroux JC,Damas J,et,al.Biocompatibility of thermosensitive chitosan-based hydrogels:an in vivo experimental approach to injectable biomaterials.Biomaterials.2002,23(13):2717-22.
72.Souyris F,Pellequer C,Payrot C,et,al.Coral,a new biomedical material.Experimental and first clinical investigations on Madreporaria.J Maxillofac Surg.1985,13(2):64-9.
73.郑有华,任材年,陈小华,等.复合珊瑚骨修复颌骨实验性骨缺损.临床口腔医学杂志,1996,12(03):143-145.
74.张森林,毛天球,王会信,等.重组人骨形成蛋白2-珊瑚复合人工骨修复骨质缺损的实验研究.中华口腔医学杂志,1998,33(01):13-14.
75.韩亮,毛天球,戴毅敏,等.珊瑚转化多孔羟基磷灰石复合rhBMP-2人工骨的动物实验研究.实用口腔医学杂志,1999,15(04):46-48.
76.Jarcho M.Calcium phosphate ceramics as hard tissue prosthetics.Clin Orthop Relat Res.1981,(157):259-78..
77.Renooij W,Hoogendoorn HA,Visser WJ,et,al.Bioresorption of ceramic strontium-85-1abeled calcium phosphate implants in dog femora.A pilot study to quantitate bioresorption of ceramic implants of hydroxyapatite and tricalcium orthophosphate in vivo.Clin Orthop Relat Res.1985,(197):272-85.
78.Hollinger JO,Battistone GC.Biodegradable bone repair materials.Synthetic polymers and ceramics.Clin Orthop Relat Res.1986,(207):290-305.
79.Levin MP,Getter L,Cutright DE,Bhaskar SN.Biodegradable ceramic in periodontal defects.Oral Surg Oral Med Oral Pathol.1974,38(3): 344-51.
80.王海亭,陈磊,金雪玲,等.羟基磷灰石(HAP)材料在生物材料中的研究与开发.山东陶瓷.2002,25(2):3-8.
81.王莉丽,赵康,陈永楠.凝胶成型法制备多孔羟基磷灰石生物陶瓷.硅酸盐通报,2005,24(2):110-112.
82.孙劲东,徐韵涛.SD大鼠骨髓基质细胞与多孔羟基磷灰石陶瓷支架体外粘附的实验研究.海南医学,2005,16(10):140-141.
83.Casabona F,Martin I,Muraglia A,et,al.Prefabricated engineered bone flaps:an experimental model of tissue reconstruction in plastic surgery.Plast Reconstr Surg.1998,101(3):577-81.
84.Kon E,Muraglia A,Corsi A,et,al.Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones.J Biomed Mater Res.2000,49(3):328-37.
85.郭昭庆,党耕町,王志国,等.种植骨髓基质细胞的骨组织工程学研究.中华外科杂志,1999,37(07):10-13.
86.Sayer M,Stratilatov AD,Reid J,et,al.Structure and composition of silicon stabilized tricalcium phosphate.Biomaterials.2003,24(3):369-82.
87.Reddi AH,Huggins CB.Influence of geometry of transplanted tooth and bone on transformation of fibroblasts.Proc Soc Exp Biol Med.1973,143(3):634-7.
88.Handschel J,Wiesmann HP,Stratmann U,et,al.TCP is hardly resorbed and not osteoconductive in a non-loading calvarial model.Biomaterials.2002,23(7):1689-95.
89.Pilliar RM,Filiaggi MJ,Wells JD,et,al.Porouscalcium polyphosphate scaffolds for bone substitute applications—in vitro characterization.Biomaterials.2001,22(9):963-72.
90.颜晓慧,洪华容,张晓.可降解聚合物用作骨组织工程细胞外基质材料的研究进展.北京生物医学工程,2000,19(02):123-128.
91.Vesala AL,Kallioinen M,T(o|¨)rm(a|¨)l(a|¨) P,et,al.Bone tissue engineering:treatment of cranial bone defects in rabbits using self-reinforced poly-L,D-lactide 96/4 sheets.J Craniofac Surg.2002,13(5):607-13.
92.Ishaug SL,Crane GM,Miller MJ,et,al.Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds.J Biomed Mater Res.1997,36(1):17-28.
93.Sikavitsas VI,Bancroft GN,Holtorf HL,et,al.Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces.Proc Natl Acad Sci U S A.2003,100(25):14683-8.
94.Agrawal CM,Best J,Heckman JD,et,al.Protein release kinetics of a biodegradable implant for fracture non-unions.Biomaterials.1995,16(16):1255-60.
95.Zellin G,Linde A.Importance of delivery systems for growth-stimulatory factors in combination with osteopromotive membranes.An experimental study using rhBMP-2 in rat mandibular defects.J Biomed Mater Res.1997,35(2):181-90.
96.Miyamoto S,Takaoka K,Okada T,et,al.Evaluation of polylactic acid homopolymers as carriers for bone morphogenetic protein Clin Orthop Relat Res.1992,(2?8):274-85..
97.Marra KG,Szem JW,Kumta PN,et,al.In vitro analysis of biodegradable polymer blend/hydroxyapatite composites for bone tissue engineering.J Biomed Mater Res.1999,47(3):324-35.
98.Saito N,Okada T,Horiuchi H,et,al.Biodegradable poly-D,L-lactic acid-polyethylene glycol block copolymers as a BMP delivery system for inducing bone.J Bone Joint Surg Am.2001,83-A Suppl 1(Pt 2):S92-8.
99.Oldham JB,Lu L,Zhu X,et,al.Biological activity of rhBMP-2 released from PLGA microspheres.J Biomech Eng.2000,122(3):289-92.
100.Murphy WL,Peters MC,Kohn DH,et,al.Sustained release of vascular endothelial growth factor from mineralized poly scaffolds for tissue engineering Biomaterials.2000,21(24):2521-7.
101.贺小兵,卢卫忠,唐康来,等.骨形成蛋白和转化生长因子-β对兔尺骨骨折愈合后生物力学性能的影响.中国修复重建外科杂志,2003,17(3):185-188.
102.Thomson RC,Yaszemski MJ,Powers JM,et,al.Hydroxyapatite fiber reinforced poly(alpha-hydroxy ester) foams for bone regeneration.Biomaterials.1998,19(21):1935-43.
103.Lin HR,Yeh YJ.Porous alginate/hydroxyapatite composite scaffolds for bone tissue engineering:preparation,characterization,and in vitro studies.J Biomed Mater Res B Appl Biomater.2004,71(1):52-65.
104.Peter SJ,Yaszemski MJ,Suggs LJ,et,al.Characterization of partially saturated poly(propylene fumarate) for orthopaedic application.J Biomater Sci Po]ym Ed.1997,8(11):893-904.
105.Mak K,Toriumi DM.Injectable filler materials for soft-tissue augmentation.Otolaryngol Clin North Am.1994 Feb;27(1):211-22.
106.Friess W.Collagen—biomaterial for drug delivery.Eur J Pharm Biopharm.1998 Mar;45(2):113-36.
107.Wu L,Zhang H,Zhang J,et,al.Fabrication of three-dimensional porous scaffolds of complicated shape for tissue engineering.Ⅰ.Compression molding based on flexible-rigid combined mold.Tissue Eng.2005 Jul-Aug;11(7-8):1105-14
108.Wu L,Jing D,Ding J.A "room-temperature" injection molding/particulate leaching approach for fabrication of biodegradable three-dimensional porous scaffolds.Biomaterials.2006 Jan;27(2):185-91.
109.段世锋,朱文,俞麟,等。一种新型可注射性组织工程水凝胶的双组分引发体系细胞毒性的负协同效应.科学通讯,2005,50(05):430-433.
110.Elisseeff J.Injectable cartilage tissue engineering.Expert Opin Biol Ther.2004 Dec;4(12):1849-59.
111.Hou,Q.P.;De Bank,P.A.;Shakesheff,K.M.,Injectable scaffolds for tissue regeneration.J.Mater.Chem.2004,14,1915-1923.
112.Basle MF,Grizon F,Pascaretti C,et,al.Shape and orientation of osteoblast-like cells(Saos-2) are influenced by collagen fibers in xenogenic bone biomaterial.J Biomed Mater Res.1998 Jun 5;40(3):350-7.
113.Shi S,Kirk M,Kahn AJ.The role of type Ⅰ collagen in the regulation of the osteoblast phenotype.J Bone Miner Res.1996Aug;11(8):1139-45.
114.Green J,Schotland S,Stauber DJ,et,al.Cell-matrix interaction in bone:type Ⅰ collagen modulates signal transduction in osteoblast-likecells.Am J Physiol.1995 May;268(5 Pt 1):C1090-103.
115.Roche S,Ronziere MC,Herbage D,et,al.Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering.Biomaterials.2001Jan;22(1):9-18.
116.Mizuno M,Shindo M,Kobayashi D,et,al.Osteogenesis by bone marrow stromal cells maintained on type Ⅰ collagen matrix gels in vivo.Bone.1997 Feb;20(2):101-7.
117.Wakitani S,Kimura T,Hirooka A,et,al.Repair of rabbit articular surfaces with allograft chondrocytes embedded in collagen gel.J Bone Joint Surg Br.1989 Jan;71(1):74-80.
118.Kang HW,Tabata Y,Ikada Y.Fabrication of porous gelatin scaffolds for tissue engineering.Biomaterials.1999 Jul;20(14):1339-44.
119.Rault I,Frei V,Herbage D,et,al.(1996) Evaluation of different chemical methods for cross-linking collagen gel,films and sponges.J Mater Sci Mater Med,7:215-221
120.Chevallay B,Abdul-Malak N,Herbage D.Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds:influence of crosslinking with diphenylphosphorylazide on matrix reorganization,growth,and biosynthetic and proteolyticactivities.J Biomed Mater Res.2000 Mar 15;49(4):448-59.
121.田卫东,乔鞠,李声伟,等.纤维蛋白粘合剂作为骨形成蛋白载体的实验研究.中国口腔种植学杂志,1999,4(1):17-20.
122.Perka C,Spitzer RS,Lindenhayn K,et,al.Matrix-mixed culture:new methodology for chondrocyte culture and preparation of cartilage transplants.J Biomed Mater Res.2000 Mar 5;49(3):305-11.
123.李征.纤维蛋白胶的作用原理和临床应用及不良反应.华西药学杂志,1999,14(1):39-41.
124.Homminga GN,Buma P,Koot HW,et,al.Chondrocyte behavior in fibrin glue in vitro.Acta Orthop Stand.1993 Aug;64(4):441-5.
125.Sims CD,Butler PE,Cao YL,et,al.Tissue engineered neocartilage using plasma derived polymer substrates and chondrocytes.Plast Reconstr Surg.1998 May;101(6):1580-5.
126.Hendrickson DA,Nixon AJ,Grande DA,et,al.Chondrocyte-fibrin matrix transplants for resurfacing extensive articular cartilage defects.J Orthop Ues.1994 Jul;12(4):485-97.
127.Ye Q,Z(u|¨)nd G,Benedikt P,et,al.Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering.Eur J Cardiothorac Surg.2000 May;17(5):587-91.
128.Ikari Y,Mulvihill E,Schwartz SM alpha 1-Proteinase inhibitor,alpha 1-antichymotrypsin,and alpha 2-macroglobulin are the antiapoptotic factors of vascular smooth muscle cells.J Biol Chem.2001 Apr 13;276(15):11798-803.
129.Meinhart J,Fussenegger M,H(o|¨)bling W.Stabilization of fibrin-chondrocyte constructs for cartilage reconstruction.Ann Plast Surg.1999 Jun;42(6):673-8.
130.池光范,候宜,候立中,等.胶原一纤维蛋白混合凝胶对关节软骨细胞合成分泌Ⅱ型胶原的组织学影响研究.第三届全国组织工程学术会议暨第一届中国国际组织工程会议论文集,成都,p 178-180
131.Peretti GM,Randolph MA,Zaporojan V,et,al.A biomechanical analysis of an engineered cell-scaffold implant for cartiiage repair.Ann Plast Surg.2001 May;46(5):533-7.
132.Draget KI,Skjak-Braek G,Smidsrφd O.Alginate based new materials.Int J Biol Macromol.1997 Aug;21(1-2):47-55.
133.Smidsrφd O,Skjak-Braek G.Alginate as immobilization matrix for cells.Trends Biotechnol.1990 Mar;8(3):71-8.
134.Kuo CK,Ma PX.Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering:part 1.Structure,gelation rate and mechanical properties.Biomaterials.2001 Mar;22(6):511-21.
I35.Majmudar G,Bole D,Goldstein SA,et,al.Bone cell culture in a three-dimensional polymer bead stabilizes the differentiated phenotype and provides evidence that osteoblastic ceils synthesize type Ⅲ collagen and fibronectin.J Bone Miner Res.1991Aug;6(8):869-81.
136.Hguselmann HJ,Fernandes RJ,Mok SS,et,al.Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads.J Cell Sci.1994 Jan;107(Pt 1):17-27.
137.Paige KT,Cima LG,Yaremchuk MJ,et,al.De novo cartilage generation using calcium alginate-chondrocyte constructs.Plast Reconstr Surg.1996 Jan;97(1):168-78.
138.Paige KT,Cima LG,Yaremchuk MJ,et,al.Injectable cartilage.Plast Reconstr Surg.1995 Nov;96(6):1390-8;discussion 1399-400.
139.Park DJ,Bong JP,Park SY,et,al.Cartilage generation using alginate-encapsulated autogenous chondrocytes in rabbits.Ann Otol Rhinol Laryngol.2000 Dec;109(12 Pt 1):1157-61.
140.Stevens MM,Qanadilo HF,Langer R,et,al.A rapid-curing alginate gel system:utility in periosteum-derived cartilage tissue engineering.Biomaterials.2004 Feb;25(5):887-94.
141.陶凯,毛天球,陈富林,等.骨髓基质成骨细胞-松质骨基质复合人工骨皮下移植成骨作用的实验研究.中华口腔医学杂志,2002 37(1):18-20.
142.Alsberg E,Anderson KW,Albeiruti A,et,al.Cell-interactive alginate hydrogels for bone tissue engineering.J Dent Res.2001Nov;80(11):2025-9.
143.孙磊,胡蕴玉,窦榆生,等.骨髓基质细胞在藻酸盐凝胶中的生物学行为.中国矫形外科杂志,2002,9(02):54-56.
144.Shang Q,Wang Z,Liu W,Shi Y,et,al.Tissue-engineered bone repair of sheep cranial defects with autologous bone marrow stromal cells.J Craniofac Surg.2001 Nov;12(6):586-93.
145.羊书勇,杨维东,雷德林,等.可注射组织工程骨的初步研究.实用口腔医学杂志,2001,17(04):271-273.
146.翁雨来,蔡霞,商庆新,等.骨髓基质干细胞-改良藻酸钙凝胶修复颅骨缺损的实验研究.上海口腔医学,2001,10(1):35-38.
147.Mi FL,Lin YM,Wu YB,et,al.Chitin/PLGA blend microspheres as a biodegradable drug-delivery system:phase-separation,degradation and release behavior.Biomaterials.2002 Aug;23(15):3257-67.
148.Klokkevold PR,Vandemark L,Kenney EB,et,al.Osteogenesis enhanced by chitosan(poly-N-acetyl glucosaminoglycan) in vitro.J Periodontol.1996 Nov;67(11):1170-5.
149.Madihally SV,Matthew HW.Porous chitosan scaffolds for tissue engineering.Biomaterials.1999 Jun;20(12):1133-42.
150.Suh JK,Matthew HW.Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering:a review.Biomaterials.2000 Dec;21(24):2589-98.
151.Boland T,Mironov V,Gutowska A,et,al.Cell and organ printing 2:fusion of cell aggregates in three-dimensional gels.Anat Rec A Discov Mol Cell Evol Biol.2003 Jun;272(2):497-502.
152.Vercruysse KP,Marecak DM,Marecek JF,et,al.Synthesis and in vitro degradation of new polyvalent hydrazide cross-linked hydrogels of hyaluronic acid.Bioconjug Chem.1997 Sep-Oct;8(5):686-94.
153.Girotto D,Urbani S,Brun P,et,al.Tissue-specific gene expression in chondrocytes grown on three-dimensional hyaluronic acid scaffolds.Biomaterials.2003 Aug;24(19):3265-75.
154.Duranti F,Salti G,Bovani B,et,al.Injectable hyaluronic acid gel for soft tissue augmentation.A clinical and histological study.Dermatol Surg.1998 Dec;24(12):1317-25.
155.Barbucci R,Lamponi S,Borzacchiello A,et,al.Hyaluronic acid hydrogel in the treatment of osteoarthritis.Biomaterials.2002Dec;23(23):4503-13.
156.Aigner J,Tegeler J,Hutzler P,et,al.Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester. J Biomed Mater Res. 1998 Nov;42(2):172-81.
157. Dillon GP, Yu X, Sridharan A, et, al. The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold. J Biomater Sci Polym Ed. 1998;9(10):1049-69.
158. Diduch DR, Jordan LC, Mierisch CM, et,al. Marrow stromal cells embedded in alginate for repair of osteochondral defects. Arthroscopy. 2000 Sep;16(6):571-7
159. Liang HF, Hong MH, Ho RM, et, al. Novel method using a temperature-sensitive polymer (methylcellulose) to thermally gel aqueous alginate as a pH-sensitive hydrogel. Biomacromolecules. 2004 Sep-Oct;5(5):1917-25.
160. Choi HS, Yamamoto K, Ooya T, et,al. Synthesis of poly-grafted dextrans and their and thermosensitive hydrogelation with cyclodextrins. Chemphyschem. 2005 Jun 13;6(6):1081-6.
1.Oreffo RO,Triffitt JT.Future potentials for using osteogenic stem cells and biomaterials in orthopedics.Bone.1999,25(2 Suppl):5S-9S.
2.Kadiyala S,Young RG,rhiede MA,et,al.Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro.Cell Transplant.1997,6(2):125-34.
3.刘延青,娄思权.骨髓基质中的骨源性干细胞.中华骨科杂志,2000,20(2):49-51.
1.刘国廉主编.细胞毒理学.第一版.北京军事医学科学出版社,2000,367-370.
2.陈治清主编.口腔材料学.第二版.北京人民卫生出版社,2000,35-42.
3.Fragonas E,Valente M,Pozzi-Mucelli M,et,al.Articu]ar cartilage repair in rabbits by using suspensions of allogenic chondrocytes in alginate.Biomaterials.2000,21(8):795-801.
4.Marijnissen WJ,van Osch GJ,Aigner J,et al.Tissue-engineered cartilage using Serially passaged articular chondrocytes.Chondrocytes in alginate,combined in vivo with a synthetic(E210) or biologic biodegradable carrier(DBM).Biomaterials.2000,21(6):571-80.
5.Kuo CK,Ma PX.Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering:part 1.Structure,gelation rate and mechanical properties.Biomaterials.2001,22(6):511-21.
1.赵谋明,李敏,孙哲浩,等.明胶与海藻酸钠的相互作用及其应用.食品与发酵工业,2000,26(03)10-14.
2.郑洪河,张虎成,夏志清,等.海藻酸钠溶液的粘度性质与流变学特征.河南师范大学学报(自然科学版),1997,25(02),55-59.
3.严金龙,孙汝东,柏云杉,聂福礼,王肇福.明胶的粘度行为和谱学特征.皮革化工,1999,16(06),1-3.
1.Paige KT,Cima LG,Yaremchuk MJ,et al.Injectable cartilage.Plast Reconstr Surg.1995,96(6):1390-8.
2.Shang Q,Wang Z,Liu W,et,al.Tissue-engineered bone repair of sheep cranial defects with autologous bone marrow stromal cells.J Craniofac Surg.2001,12(6):586-93.
3.施绮云,宁国伯.对藻酸钠消毒方法的研究.中国消毒学杂志,1998,15(02),28-31.
1.Perka C,Spitzer RS,Lindenhayn K,et,al.Matrix-mixed culture:new methodology for chondrocyte culture and preparation of cartilage transplants.J Biomed Mater Res.2000,49(3):305-11.
2.Wang L,Shelton RM,Cooper PR,et,al.Evaluationof sodium alginate for bone marrow cell tissue engineering.Biomaterials.2003 24(20):3475-81.
3.王敏,翁雨来,胡晓洁,等。组织工程技术修复犬牙槽骨缺损的实验研究。中华医学杂志.2003,83(15),55-60.
1.GB/T 16886.1-2001.医疗器械生物学评价第1部分:评价与试验
1.Gauthier O,Bouler JM,Weiss P,et al.Kinetic study of bone ingrowth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes.J Biomed Mater Res.1999,47(1):28-35.
2.Gauthier O,Boix D,Grimandi G,et al.A new injectable calcium phosphate biomaterial for immediate bone filling of extraction sockets:a preliminary study in dogs.J Periodontol.1999,70(4):375-83.
3.杨维东.口腔颌面骨替代材料与骨组织工程.实用口腔医学杂志,1999,15(06):469-471.
1.Schmitz JP,Hollinger JO.The critical size defect as an experimental model for craniomandibulofacial nonunions.Clin Orthop Relat Res.1986,(205):299-308.
2.Hollinger JO,Kleinschmidt JC.The critical size defect as an experimental model to test bone repair materials.J Craniofac Surg.1990,1(1):60-8.