浙江省甲3亚型流感流行与猪型流感的关系探讨
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摘要
背景和目的:
流行性感冒(influenza)是由流感病毒(Influenza Virus)引起的,具有高度传染性的急性呼吸道传染病。由于流感病毒基因的高度变异性,使该病在人群中发病率高且易发生流行。2009年春季开始的甲型H1N1流感流行再次使流感病毒成为全球关注的热点。甲型流感病毒,是危害人类健康的重要病原体之一,同时中国地区,又是世界上流感变异株的发源地之一,所以对其变异规律进行深入细致地分析研究,在流感的防治工作中具有极为重要的意义。猪流感(Swine influenza,SI)是由猪流感病毒(Swine influenza virus,SIV)引起的急性猪呼吸道传染病。猪具有同时感染禽和人流感病毒的能力,被认为是新亚型流感病毒产生的中间宿主和“基因混合器”。而且,猪流感病毒具有感染人和禽的能力,因此,猪流感具有重要的兽医和人类公共卫生学意义。本研究基于这一背景,就浙江省近十年来流感流行的甲型H3N2代表株,与猪型H3N2株在主要抗原基因间进行比较分析,探讨浙江省甲3亚型流感病毒与猪流感病毒之间的关系。
材料和方法:
对浙江省近十年来流感流行期间分离的甲3亚型流感代表性毒株,采用逆转录-聚合酶链反应(Reverse transcription-Polymerase chain reaction ,RT-PCR)扩增病毒血凝素(Haemagglutinin, HA)和神经氨酸酶(Neuraminidase, NA)基因,进行核苷酸序列测定,并与其它同期的人源及猪源H3N2流感毒株的相应序列用BioEdit(version 5.0.9.1)和MEGA4.0软件作同源性与进化树分析。(其它同期的人源及猪源H3N2流感毒株的HA1、NA序列,由美国NCBI的GenBank下载。)
结果:
浙江省近十年来甲3亚型流感流行中的代表株A3/浙江(Zhejiang,ZJ)/10/98、A3/Zhejiang/6/99、A3/Zhejiang/8/02与猪流感A3/SW/安大略( Ontario,ON)/130/97、A3/SW/香港(Hongkong,HK)/4361/99、A3/SW/Hongkong/74/02在HA1区的同源性分别为99.1%、99.4%、99.4%;在NA区,A3/Zhejiang/10/98、A3/Zhejiang/6/99、A3/Zhejiang/8/02与猪流感A3/SW/Ontario/130/97、A3/SW/Henan/S4/01、A3/SW/HongKong/411//02的同源性分别达到98.2%、99.3%、99.3%。二者之间均呈现很高的同源性,有的远远高于它与同期人流感毒株的同源性,在基因系统进化树上也呈现这一状况。
结论:
浙江省近十年来甲3亚型流感流行的毒株与猪流感的某些毒株之间存在着密切的联系,猪流感与人流感之间的关系值得深入研究。
Background and objectives:
Influenza is an acute respiratory disease which was caused by influenza virus. Because of high genetic variation of the virus, it infected the population again and again with high incidence. The global epidemic of influenza A H1N1 virus in Spring 2009 become the world’s attention once again. Because influenza A virus remains an important pathogen which threatens health of human in long time and China is the main resources of the new viruses, so deeply understanding the evolution and variation rule of influenza virus has very important meaning for prevention of influenza. Swine influenza is an acute respiratory disease which was caused by swine influenza virus. Pig is susceptible to avian and human influenza viruses and has been proposed to be intermediate host or“mixing vessel”for the generation of pandemic influenza virus. On the other hand, swine influenza virus could infect people and avian. Swine influenza surveillance is of great significance for veterinary and public health. In this assay, we investigated the phylogenetic relationship between swine influenza A/H3N2 virus and the representative strains of human influenza A/H3N2 virus isolated in the last ten years, through comparing the sequences within HA and NA genes.
Material and methods:
HA、NA gene of the representative strains of Influenza A/H3N2 isolated in the last ten years in Zhejiang Province were amplified by reverse transcription polymerase chain reaction (RT-PCR). PCR products were purified and sequenced, other HA1、NA sequences of H3N2 human and swine influenza virus in the corresponding period of time were downloaded from NCBI GenBank, then data were aligned and used to construct phylogenetic tree with biologic software bioEdit(version 5.0.9.1) and MEGA(version4.0).
Results:
The homologies on the HA domain between human representative strains (A/Zhejiang/10/98, A/Zhejiang/6/99 and A/Zhejiang/8/02) and the swine strains (A/SW/Ontario/130/97、A/SW/Hongkong/4361/99 and A/SW/Hongkong/74/02) are 99.1%, 99.4% and 99.4% respectively, and based on the NA gene, the homologies between human strains (A/Zhejiang/10/98, A/Zhejiang/6/99 and A/Zhejiang/8/02) and the swine strains (A/SW/Ontario/130/97, A3/SW/Henan/S4/01 and A3/SW/HongKong/411//02) are 98.2%,99.3% and 99.3% respectively. These showed that the two types of Influenza viruses were highly homologue, and even some of their homologies were higher than that amongst the contemporary human influenza A/H3N2 strains. The same results were shown in the phylogenetic tree.
Conclusions:
The human influenza A/H3N2 virus isolated in the last ten years in Zhejiang Province closely associated with some of the swine influenza virus strains, and their relationship should be further studied.
流行性感冒(influenza)是由流感病毒(Influenza Virus)引起的,具有高度传染性的急性呼吸道传染病。由于流感病毒基因的高度变异性,使该病在人群中发病率高且易发生流行。2009年春季开始的甲型H1N1流感流行再次使流感病毒成为全球关注的热点。甲型流感病毒,是危害人类健康的重要病原体之一,同时中国地区,又是世界上流感变异株的发源地之一,所以对其变异规律进行深入细致地分析研究,在流感的防治工作中具有极为重要的意义。猪流感(Swine influenza,SI)是由猪流感病毒(Swine influenza virus,SIV)引起的急性猪呼吸道传染病。猪具有同时感染禽和人流感病毒的能力,被认为是新亚型流感病毒产生的中间宿主和“基因混合器”。而且,猪流感病毒具有感染人和禽的能力,因此,猪流感具有重要的兽医和人类公共卫生学意义。本研究基于这一背景,就浙江省近十年来流感流行的甲型H3N2代表株,与猪型H3N2株在主要抗原基因间进行比较分析,探讨浙江省甲3亚型流感病毒与猪流感病毒之间的关系。
材料和方法:
对浙江省近十年来流感流行期间分离的甲3亚型流感代表性毒株,采用逆转录-聚合酶链反应(Reverse transcription-Polymerase chain reaction ,RT-PCR)扩增病毒血凝素(Haemagglutinin, HA)和神经氨酸酶(Neuraminidase, NA)基因,进行核苷酸序列测定,并与其它同期的人源及猪源H3N2流感毒株的相应序列用BioEdit(version 5.0.9.1)和MEGA4.0软件作同源性与进化树分析。(其它同期的人源及猪源H3N2流感毒株的HA1、NA序列,由美国NCBI的GenBank下载。)
结果:
浙江省近十年来甲3亚型流感流行中的代表株A3/浙江(Zhejiang,ZJ)/10/98、A3/Zhejiang/6/99、A3/Zhejiang/8/02与猪流感A3/SW/安大略( Ontario,ON)/130/97、A3/SW/香港(Hongkong,HK)/4361/99、A3/SW/Hongkong/74/02在HA1区的同源性分别为99.1%、99.4%、99.4%;在NA区,A3/Zhejiang/10/98、A3/Zhejiang/6/99、A3/Zhejiang/8/02与猪流感A3/SW/Ontario/130/97、A3/SW/Henan/S4/01、A3/SW/HongKong/411//02的同源性分别达到98.2%、99.3%、99.3%。二者之间均呈现很高的同源性,有的远远高于它与同期人流感毒株的同源性,在基因系统进化树上也呈现这一状况。
结论:
浙江省近十年来甲3亚型流感流行的毒株与猪流感的某些毒株之间存在着密切的联系,猪流感与人流感之间的关系值得深入研究。
Background and objectives:
Influenza is an acute respiratory disease which was caused by influenza virus. Because of high genetic variation of the virus, it infected the population again and again with high incidence. The global epidemic of influenza A H1N1 virus in Spring 2009 become the world’s attention once again. Because influenza A virus remains an important pathogen which threatens health of human in long time and China is the main resources of the new viruses, so deeply understanding the evolution and variation rule of influenza virus has very important meaning for prevention of influenza. Swine influenza is an acute respiratory disease which was caused by swine influenza virus. Pig is susceptible to avian and human influenza viruses and has been proposed to be intermediate host or“mixing vessel”for the generation of pandemic influenza virus. On the other hand, swine influenza virus could infect people and avian. Swine influenza surveillance is of great significance for veterinary and public health. In this assay, we investigated the phylogenetic relationship between swine influenza A/H3N2 virus and the representative strains of human influenza A/H3N2 virus isolated in the last ten years, through comparing the sequences within HA and NA genes.
Material and methods:
HA、NA gene of the representative strains of Influenza A/H3N2 isolated in the last ten years in Zhejiang Province were amplified by reverse transcription polymerase chain reaction (RT-PCR). PCR products were purified and sequenced, other HA1、NA sequences of H3N2 human and swine influenza virus in the corresponding period of time were downloaded from NCBI GenBank, then data were aligned and used to construct phylogenetic tree with biologic software bioEdit(version 5.0.9.1) and MEGA(version4.0).
Results:
The homologies on the HA domain between human representative strains (A/Zhejiang/10/98, A/Zhejiang/6/99 and A/Zhejiang/8/02) and the swine strains (A/SW/Ontario/130/97、A/SW/Hongkong/4361/99 and A/SW/Hongkong/74/02) are 99.1%, 99.4% and 99.4% respectively, and based on the NA gene, the homologies between human strains (A/Zhejiang/10/98, A/Zhejiang/6/99 and A/Zhejiang/8/02) and the swine strains (A/SW/Ontario/130/97, A3/SW/Henan/S4/01 and A3/SW/HongKong/411//02) are 98.2%,99.3% and 99.3% respectively. These showed that the two types of Influenza viruses were highly homologue, and even some of their homologies were higher than that amongst the contemporary human influenza A/H3N2 strains. The same results were shown in the phylogenetic tree.
Conclusions:
The human influenza A/H3N2 virus isolated in the last ten years in Zhejiang Province closely associated with some of the swine influenza virus strains, and their relationship should be further studied.
引文
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[2]李立明.流行病学[M].第5版.北京:人民卫生出版社,2004,475-480.
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