矿质元素调控烟草抗PVY~N生理生化及分子机制研究
|
摘要
本文以栽培烟草品种K 326(Nicotiana tabacum L.CV.K326)为试材,对矿质元素调控烟草抗PVY~N的作用进行了研究,从营养抗性角度揭示了矿质元素可通过调控烟草自身生理生化特性以提高植株的抗病毒能力,并明确了矿质营养调控烟草对病毒抗病性的分子机制,为矿质营养调控植物抗性利用奠定了重要理论基础。本论文主要研究结果如下:
1.明确了镁、铁、铜、硼素可提高烟草抗病毒能力,几种元素处理均使植株病害减轻及病毒含量降低,几种元素调控烟草抗PVY~N的最佳浓度分别为240·L~(-1)、33.6mg·L~(-1)、0.8 mg·L~(-1)和20 mg·L~(-1)。本试验证实Mg3(240 mg·L~(-1))、Fe4(33.6 mg·L~(-1))、Cu3(0.8 mg·L~(-1))及B3(20 mg·L~(-1))处理的烟株病情指数显著低于接种对照,并且该四种元素浓度处理均能降低烟株体内病毒含量,由此可见Mg3、Fe4、Cu3及B3处理对PVY~N有较好的抗性,从表观上看,亦能延迟脉坏死症状的发生;不同元素处理24、72、120、168 h后接种PVY~N植株体内病毒含量差别显著,其中镁、铁、硼三种元素处理后24 h接种效果较好,能明显降低病毒含量,而铜元素处理24、72、120 h后烟株体内的病毒含量相近。因此,本试验选定喷施元素后24 h接种PVY~N为最佳作用时间。
2.采用上述试验筛选的最佳铁、铜、镁、硼浓度处理,明确了矿质元素可通过调节烟株生理生化特性以提高抗性。研究表明:元素处理后,提高了烟株体内可溶性蛋白、脯氨酸、可溶性糖、叶绿素含量,促进PAL、POD、CAT等防御酶的活性,提高光合效率,降低植株体内有害物质丙二醛的含量,减少电解质的外渗,提高烟株木质化程度;喷施矿质元素也可在短期内迅速提高活性氧的含量,活性氧的迸发能刺激寄主植株防御反应的应答;而与叶脉褐变有关的PPO、总酚、类黄酮等物质则表现为前期高于对照后期低于对照烟株,以减少这些物质的破坏作用,因为过多的PPO、总酚、类黄酮会加重褐变。由此可见,喷施矿质元素一方面能增强烟株抗病性,同时也可减轻感病植株症状,减轻PVY~N对烟株的伤害。
3.采用气相色谱(GC)、高效液相色谱(HPLC)的测定方法,研究发现铜素能调控感染PVY~N的烟株体内抗病信号物质水杨酸积累和乙烯释放。试验结果表明,水杨酸含量在接种后第15 d时达到最大值,为接种对照的1.3倍:而乙烯释放量在3～9 d内均高于接种对照,在第12 d时乙烯释放量显著低于接种对照,分析认为前期Cu素处理后诱导了乙烯释放的增强,进行抗性信号的传递,后期接种对照严重发病是其释放大量乙烯的直接原因。因此,喷施Cu素后可以诱导烟草植株产生抗病性,增强烟草对PVY~N侵染的抵抗力,并且诱导了相关抗性信号的传递。
4.明确了铁、铜、镁素具有改变钙离子浓度、调节钙信使系统的功能,使与抗性信号传导相关的钙调素(CaM)、磷脂酶C(PLC)和蛋白激酶C(PKC)活性提高。通过测定不同元素调节钙离子的动态变化发现,元素处理后接种与未接种的烟株体内全钙及可溶性钙含量均有不同程度的增加,同时细胞膜上的Ca~(2+)-ATPase和钙离子受体NADKase明显被激活,研究发现细胞内钙离子浓度升高与二者活性增加密切相关;研究发现,铜素处理后可诱导烟株体内CaM、PKC活性不同程度的提高,且均发生在较早阶段,而磷脂酶C(PLC)的活性变化随钙离子浓的变化而变化,二者呈负相关。由此可见,矿质元素可通过对钙离子浓度的调节,进而参与钙信使系统的抗性信号传递。
5.采用SYBR GreenⅠ作为荧光染料的Real-time PCR方法,明确了铜、镁素能调控烟株体内蛋白激酶级联(MAPKs)信号转导途径的关键基因WIPK、SIPK及抗病信号通路末端PR1基因的过量表达,进一步揭示了矿质营养调控烟草对病毒抗病性的分子机制。本研究发现铜、镁元素分别处理及对照烟株中均有WIPK、SIPK及PR1基因的表达,但表达量有所差异,元素处理的烟株中表达量要高于对照烟株,接种处理的烟株表达量要高于未接种的烟株。其中,除SIPK最大表达量在96 h外,WIPK和PR1基因最大表达量均出现在168 h。由此可见,铜、镁元素处理能诱导信号通路中关键基因的过量表达,使烟株产生抗性,提高植株对病毒的抵抗力。
The regulating action of mineral elements on tobacco plants for resistant to PVY~N was studied by using tobacco variety K326.The mineral elements can improve the tobacco resistant to PVY~N through promoting the physiological and biochemical property.The resistant molecular mechanism of tobacco regulated with mineral elements to PVY~N were revealed in this paper.
1.Magnesium,iron,copper and boron can improve the tobacco resistant to PVY~N, which made the disease symptom lightened and the content of virus decreased at the best concentration of Mg 3(240 mg·L~(-1)),Fe4(33.6 mg·L~(-1)),Cu3(0.8 mg·L~(-1)) and B3(20 mg·L~(-1)). Effect on content of virus in tobacco which treated with different mineral elements at 24,72, 120,168 h showed that,the best induced time to tobacco treated with iron,magnesium and boron was 24 h,and that of copper treatment having no difference between 24,72 and 120 h. In a word,24 h was the best induced time to tobacco treated different elements.
2.The Magnesium,iron,copper and boron can improve the tobacco resistant to PVY~N through promoting the physiological and biochemical property.The results showed that the activity of PAL,POD,CAT and the content of proline,soluble sugar,chlorophyll and lignin in tobacco leaves which inoculated with PVY~N after spraying mineral elements were increased,the content of leakage rate and MDA of electrolyte restrained.Those indicated that the induced resistance was promoted and the symptom was lightened.Furthermore,the production rate of activated oxygen raised sharply in a short time after treatment,those indicated that the induce resistance of tobacco was induced.Besides,the activity of PPO and content of total phenol and flavonoid,which closely linked to vein necrosis,were higher than those of the control in 12 days to resistant PVY~N,but much lower than those of the control when the vein necrosis came severity,which showed that the degree of vein browning was related to the PPO activity and the content of total phenol and flavonoid decreasing.In conclusion spraying iron can induce tobacco plant resistance to PVY~N.
3.Content of salicylic acid and ethylene in tobacco were increased by spraying mineral elements.The results showed that the content of salicylic acid(SA) increased 1.3 times in 15th after treatment.The release of ethylene(ETH) was much higher than that of control in a short time.At the 12~(th) day,ETH released of control was sharply increased,which was much higher than that of copper treatment,because the signal pathway in tobacco leaves was activated when treated with copper solution in early time.But the symptom of control was seriously at later time,which is the factor of ETH released rose.In conclusion spraying copper solution can induce tobacco plant resistance to PVY~N and activate the signal pathway.
4.The calcium system was regulated by changing of Ca2~+ in tobacco treated with mineral elements,and the activity of CaM,PLC and PKC which related with signal transduction promoted.The effect of iron,copper and magnesium solution on the Calcium signal pathway had been preliminarily identified.Consequently,there has a close relative between the content of Ca~(2+) and Ca~(2+) -ATPase and NADKase activity in tobacco leaves. Compared with those of control treatments,the content of Ca~(2+) and total calcium was increased in different time,Ca~(2+) -ATPase and NADKase activated simultaneity.Through the method of ELISA,the activity of CaM and PKC in tobacco which treated with mineral elements was promoted in early time.But there has a a negative correlation between PLC and the content of Ca~(2+).So that the calcium signal pathway could adjust by mineral elements through altering the content of Ca~(2+).
5.Take SYBR Green I as the intercalating fluorescence dye in Real-time PCR,the overexpression of WIPK and SIPK,which were the key genes of MAPK cascades,and the resistant gene PR1 in tobacco treated with copper and magnesium solution were studied.The results showed that,the expression of WIPK、SIPK and PR1 were appeared in tobacco of all treatment and control.But the amount of expression in tobacco treated with copper and magnesium solution were much higher than that of control.Except the expression levels of SIPK largest in the 96h,WIPK and PR1 expressions were both the largest in the 168h.So the key genes over expression of signal pathway in tobacco were induced by spraying copper and magnesium solution,the system resistance of tobacco to PVY~N appeared.
1.明确了镁、铁、铜、硼素可提高烟草抗病毒能力,几种元素处理均使植株病害减轻及病毒含量降低,几种元素调控烟草抗PVY~N的最佳浓度分别为240·L~(-1)、33.6mg·L~(-1)、0.8 mg·L~(-1)和20 mg·L~(-1)。本试验证实Mg3(240 mg·L~(-1))、Fe4(33.6 mg·L~(-1))、Cu3(0.8 mg·L~(-1))及B3(20 mg·L~(-1))处理的烟株病情指数显著低于接种对照,并且该四种元素浓度处理均能降低烟株体内病毒含量,由此可见Mg3、Fe4、Cu3及B3处理对PVY~N有较好的抗性,从表观上看,亦能延迟脉坏死症状的发生;不同元素处理24、72、120、168 h后接种PVY~N植株体内病毒含量差别显著,其中镁、铁、硼三种元素处理后24 h接种效果较好,能明显降低病毒含量,而铜元素处理24、72、120 h后烟株体内的病毒含量相近。因此,本试验选定喷施元素后24 h接种PVY~N为最佳作用时间。
2.采用上述试验筛选的最佳铁、铜、镁、硼浓度处理,明确了矿质元素可通过调节烟株生理生化特性以提高抗性。研究表明:元素处理后,提高了烟株体内可溶性蛋白、脯氨酸、可溶性糖、叶绿素含量,促进PAL、POD、CAT等防御酶的活性,提高光合效率,降低植株体内有害物质丙二醛的含量,减少电解质的外渗,提高烟株木质化程度;喷施矿质元素也可在短期内迅速提高活性氧的含量,活性氧的迸发能刺激寄主植株防御反应的应答;而与叶脉褐变有关的PPO、总酚、类黄酮等物质则表现为前期高于对照后期低于对照烟株,以减少这些物质的破坏作用,因为过多的PPO、总酚、类黄酮会加重褐变。由此可见,喷施矿质元素一方面能增强烟株抗病性,同时也可减轻感病植株症状,减轻PVY~N对烟株的伤害。
3.采用气相色谱(GC)、高效液相色谱(HPLC)的测定方法,研究发现铜素能调控感染PVY~N的烟株体内抗病信号物质水杨酸积累和乙烯释放。试验结果表明,水杨酸含量在接种后第15 d时达到最大值,为接种对照的1.3倍:而乙烯释放量在3～9 d内均高于接种对照,在第12 d时乙烯释放量显著低于接种对照,分析认为前期Cu素处理后诱导了乙烯释放的增强,进行抗性信号的传递,后期接种对照严重发病是其释放大量乙烯的直接原因。因此,喷施Cu素后可以诱导烟草植株产生抗病性,增强烟草对PVY~N侵染的抵抗力,并且诱导了相关抗性信号的传递。
4.明确了铁、铜、镁素具有改变钙离子浓度、调节钙信使系统的功能,使与抗性信号传导相关的钙调素(CaM)、磷脂酶C(PLC)和蛋白激酶C(PKC)活性提高。通过测定不同元素调节钙离子的动态变化发现,元素处理后接种与未接种的烟株体内全钙及可溶性钙含量均有不同程度的增加,同时细胞膜上的Ca~(2+)-ATPase和钙离子受体NADKase明显被激活,研究发现细胞内钙离子浓度升高与二者活性增加密切相关;研究发现,铜素处理后可诱导烟株体内CaM、PKC活性不同程度的提高,且均发生在较早阶段,而磷脂酶C(PLC)的活性变化随钙离子浓的变化而变化,二者呈负相关。由此可见,矿质元素可通过对钙离子浓度的调节,进而参与钙信使系统的抗性信号传递。
5.采用SYBR GreenⅠ作为荧光染料的Real-time PCR方法,明确了铜、镁素能调控烟株体内蛋白激酶级联(MAPKs)信号转导途径的关键基因WIPK、SIPK及抗病信号通路末端PR1基因的过量表达,进一步揭示了矿质营养调控烟草对病毒抗病性的分子机制。本研究发现铜、镁元素分别处理及对照烟株中均有WIPK、SIPK及PR1基因的表达,但表达量有所差异,元素处理的烟株中表达量要高于对照烟株,接种处理的烟株表达量要高于未接种的烟株。其中,除SIPK最大表达量在96 h外,WIPK和PR1基因最大表达量均出现在168 h。由此可见,铜、镁元素处理能诱导信号通路中关键基因的过量表达,使烟株产生抗性,提高植株对病毒的抵抗力。
The regulating action of mineral elements on tobacco plants for resistant to PVY~N was studied by using tobacco variety K326.The mineral elements can improve the tobacco resistant to PVY~N through promoting the physiological and biochemical property.The resistant molecular mechanism of tobacco regulated with mineral elements to PVY~N were revealed in this paper.
1.Magnesium,iron,copper and boron can improve the tobacco resistant to PVY~N, which made the disease symptom lightened and the content of virus decreased at the best concentration of Mg 3(240 mg·L~(-1)),Fe4(33.6 mg·L~(-1)),Cu3(0.8 mg·L~(-1)) and B3(20 mg·L~(-1)). Effect on content of virus in tobacco which treated with different mineral elements at 24,72, 120,168 h showed that,the best induced time to tobacco treated with iron,magnesium and boron was 24 h,and that of copper treatment having no difference between 24,72 and 120 h. In a word,24 h was the best induced time to tobacco treated different elements.
2.The Magnesium,iron,copper and boron can improve the tobacco resistant to PVY~N through promoting the physiological and biochemical property.The results showed that the activity of PAL,POD,CAT and the content of proline,soluble sugar,chlorophyll and lignin in tobacco leaves which inoculated with PVY~N after spraying mineral elements were increased,the content of leakage rate and MDA of electrolyte restrained.Those indicated that the induced resistance was promoted and the symptom was lightened.Furthermore,the production rate of activated oxygen raised sharply in a short time after treatment,those indicated that the induce resistance of tobacco was induced.Besides,the activity of PPO and content of total phenol and flavonoid,which closely linked to vein necrosis,were higher than those of the control in 12 days to resistant PVY~N,but much lower than those of the control when the vein necrosis came severity,which showed that the degree of vein browning was related to the PPO activity and the content of total phenol and flavonoid decreasing.In conclusion spraying iron can induce tobacco plant resistance to PVY~N.
3.Content of salicylic acid and ethylene in tobacco were increased by spraying mineral elements.The results showed that the content of salicylic acid(SA) increased 1.3 times in 15th after treatment.The release of ethylene(ETH) was much higher than that of control in a short time.At the 12~(th) day,ETH released of control was sharply increased,which was much higher than that of copper treatment,because the signal pathway in tobacco leaves was activated when treated with copper solution in early time.But the symptom of control was seriously at later time,which is the factor of ETH released rose.In conclusion spraying copper solution can induce tobacco plant resistance to PVY~N and activate the signal pathway.
4.The calcium system was regulated by changing of Ca2~+ in tobacco treated with mineral elements,and the activity of CaM,PLC and PKC which related with signal transduction promoted.The effect of iron,copper and magnesium solution on the Calcium signal pathway had been preliminarily identified.Consequently,there has a close relative between the content of Ca~(2+) and Ca~(2+) -ATPase and NADKase activity in tobacco leaves. Compared with those of control treatments,the content of Ca~(2+) and total calcium was increased in different time,Ca~(2+) -ATPase and NADKase activated simultaneity.Through the method of ELISA,the activity of CaM and PKC in tobacco which treated with mineral elements was promoted in early time.But there has a a negative correlation between PLC and the content of Ca~(2+).So that the calcium signal pathway could adjust by mineral elements through altering the content of Ca~(2+).
5.Take SYBR Green I as the intercalating fluorescence dye in Real-time PCR,the overexpression of WIPK and SIPK,which were the key genes of MAPK cascades,and the resistant gene PR1 in tobacco treated with copper and magnesium solution were studied.The results showed that,the expression of WIPK、SIPK and PR1 were appeared in tobacco of all treatment and control.But the amount of expression in tobacco treated with copper and magnesium solution were much higher than that of control.Except the expression levels of SIPK largest in the 96h,WIPK and PR1 expressions were both the largest in the 168h.So the key genes over expression of signal pathway in tobacco were induced by spraying copper and magnesium solution,the system resistance of tobacco to PVY~N appeared.
引文
1.蔡汉,李卫东,熊作明.2007.低温胁迫下水杨酸预处理对茉莉幼苗活性氧及保护酶的影响.植物生理科学,23(7):290-294
2.陈芳,慕小倩,梁宗锁.2007.水杨酸对附子叶斑病的诱导抗性及作用机理研.西北农业学报,16(2):245-249
3.陈力力,高必达,2006.微生物抗植物病毒活性物的研究进展,中国生物防治,22(4)255-260
4.陈齐斌,沈嘉祥.2005.抗植物病毒剂研究进展和面临的挑战与机遇.云南农业大学学报,20(4):505-511
5.陈娅斐,冯斌,赵小明.2005.MAPK级联途径在植物信号转导中的研究进展.植物学通报,22(3):357-365
6.陈英,黄敏仁,诸葛强.2002.植物抗病信号传导途径及其相互作用.南京林业大学学报,26(3):85-90
7.程伟东,江禹奉,谭贤杰.2008,水杨酸诱导玉米纹枯病系统抗性的初步研究.广西农业科学,39(6):785-787
8.崔婧.2007.水杨酸与植物抗逆性.安徽农学通报,13(9):35-38
9.单耀军,张进霞,袁洪水.2006.氨基酸铜对6种植物病原真菌生长的抑制作用.河北大学学报,26(1):30-37
10.邓文红,张风娟,金幼菊.2007.反相高效液相色谱法测定植物组织中的水杨酸.北京林业大学学报,29(1):151-154
11.董涛,张志刚,尚庆茂.2009.水杨酸和壳聚糖对黄瓜幼苗耐盐性的共诱导作用.中国蔬菜,2009(2):46-50
12.董英,胡冠娟.2006.壳聚糖对板栗褐变的抑制作用.江苏农业科学2006(4):133-135
13.杜新民,张永清.2008.施锌对石灰性褐土上小白菜光合作用及保护酶活性的影响.西北植物学报.28(6):1203-1207
14.范海延.2003.葡聚寡糖诱导蔬菜抗病性的研究.沈阳农业大学
15.冯东昕,李宝栋.1998.可溶性硅在植物抵御病害中的作用.植物病理学报.28(4):293-297
16.冯高,刘华.2008.温度和光照对PVY生物测定的影响.中国马铃薯,18(3):145-146
17.傅晓艺,刘桂茹,杨学举.2005.植物病害中的信号传导.安徽农业科学,33(5):901-903
18.关洪丹,姜新,李晔.2006.不同供铁水平对烟草与PVY~N互作关系中防御酶活性的影响.安徽农业科学.34(15):3722-3723
19.郭红莲,李丹,白雪芳.2002.壳寡糖对烟草病毒的诱导抗性研究.中国烟草科学,4:1-3
20.郭红莲.2003.壳寡糖诱导植物抗病机理及其信号转导的研究.中国科学院大连化学物理研究所.1-85
21.郭红莲等,2003.壳寡糖对棉花悬浮培养细胞活性氧的作用.中国海洋药物,(1):11-13
22.郭丽,段金秀,梁占训.2007.杜氏盐藻MAPK基因cDNA的克隆及其进化分析.草业科学,24(1):21-25
23.郭丽红,王定康,杨晓虹,陈善娜.2004.外源乙烯利对干旱胁迫过程中玉米幼苗某些抗逆生理指标的影响.云南大学学报(自然科学版),6(4):352-356
24.郭兴启,吕士恩,朱汉城.1999.烟草上马铃薯Y病毒的提纯与检测.中国烟草科学,1999(2):5-7
25.郭兴启,朱汉城,吕士恩.1998.酶标记羊抗兔间接法检测烟草马铃薯Y病毒的研究.中国烟草报,4(2):38-42
26.郭衍银,徐坤,王秀峰.2003.矿质营养与植物病害机理研究进展.甘肃农业大学学报,38(4):385-393
27.何开跃,李晓储,黄利斌.2000.冷冻胁迫对福建柏苗可溶性糖和丙二醛(MDA)含量的影响.江苏林业科技,27(6):6-8
28.黄芳,王建明,徐玉梅.2008.硼抑制灰霉病菌孢子萌发机制的初步研究.植物病理学报,38(4):370-376
29.黄国存,张继澎,孙大业.1993.对采后番茄CAM呼吸强度及乙烯产生的影响.园艺学报,20(4):403-404
30.纪秀娥,张美善,于海秋.1998.植物的硅素营养.农业与技术,1998(4):11-13
31.姜国勇,杨仁崔.2005.番茄花叶病毒移动蛋白和番茄抗病基因Tm-22的互作诱导烟草转化体程序性细胞死亡.微生物学报,45(2):301-304
32.姜华,宁淑香,杨文新.2002.壳聚糖对MDMV的防效与玉米叶片防御酶活性的关系.辽宁师范大学学报,25(1):72-75
33.李宝聚,范海延,孙艳秋.2005.葡聚六糖诱导黄瓜体内水杨酸的积累及其与抗霜霉病关系的初步研究.园艺学报,32(1):115-117
34.李怀方,张莉.钙、镁离子对烟草花叶病毒侵染的抑制作用.植物保护.20(5):2-4
35.李淮,王莱,武国凡.2006.植物抗病性的分子机制和信号传导.西北师范大学学报,46(2):113-117
36.李金玉,李冠,赵惠新.2006.植物抗病分子机制研究进展.种子,25(2):45-50
37.李金柱,吴礼树,杨玉华.2004.硼在植物细胞壁上营养机理的研究进展.中国油料作物学报,26(4):96-99
38.李娟.2007.植物钾、钙、镁素营养的研究进展.福建稻麦科技,25(1):39-42
39.李莉,任金平,曲柏宏.2007.水分胁迫对苹果梨叶片可溶性糖、脯氨酸含量的影响.吉林农业科学,32(1):51-54
40.李宁,樊守金,张增艳.2007.植物抗病相关启动子及其研究进展.植物遗传资源学报,8(2):234-239
41.李群.2005.钙依赖蛋白激酶和腺苷酸环化酶参与拟南芥响应大丽轮枝菌毒诱导信号转导过程的实验证据.中国农业大学.1-70
42.李锐,李生泉,范月仙.低温胁迫对棉花幼苗SOD、CAT活性的影响.山西农业大学,18-19
43.李艳莉,吴晓华,马耀荣.2006.反相高效液相色谱测定足光粉中苯甲酸、水杨酸的含量.佛山科学技术学院学报,24(4):68-71
44.李章海,刘登乾,田必文.2008.打顶和施用NAA对烟株生长、烟碱合成和钾素吸收的影响.烟草农学,254(9):52-55
45.李智辉,王新颖,周广柱.2007.低温胁迫下新铁炮百合幼苗叶片活性氧的产生及保护酶活性的变化.北方园艺,2007(12):106-108
46.梁和,马国瑞,石伟勇.2000.钙硼营养与果实生理及耐贮性研究进展.土壤通报,31(4):187-190
47.刘爱新,梁元存,张博.1998.植物诱导抗病性研究进展.山东农业大学学报,29(3):410-414
48.刘碧容,甄畅迪,萧洪东.2008.硼对草坪草超氧化物歧化酶活性、超氧阴离子产生速率和丙二醛含量的影响.华中农业大学学报.27(3):378-381
49.刘传和,刘岩,易干军.2008.地膜覆盖对菠萝植株有关生理指标的影响.热带作物学报,29(5):546-550
50.刘国强,吴锦程,唐朝晖.2008.枇杷采后酚类物质代谢与果肉木质化的关系.中国农学通报,24(1):247-251
51.刘强,张勇,陈受宜.2000.干旱 高盐及低温诱导的植物蛋白激酶基因.科学通报,45(6):561-566
52.刘世彪,易萍,罗奥.2008.低温胁迫对绞股蓝和五柱绞股蓝抗寒性生理指标的影响.热带作物学报,29(5):572-576
53.刘树玲.2006.稻瘟菌侵染过程中的信号传导.作物研究,2:194-198
54.刘太国,石延霞,文景芝.2003.水杨酸诱导烟草对TMV的抗性和PAL活性变化研究.植物病理学报,33(2):190-191
55.刘伟曲凌慧,刘洪庆.2008.温胁迫对葡萄保护酶和氧自由基的影响.北方园艺,(5):21-24
56.刘亚光,李海英,杨庆凯.2004.乙烯利对大豆抗性生化物质的诱导作用.哈尔滨工业大学学报,36(8):1014-1016
57.刘志文,沙爱华,王英.2005.活性氧物质在植物抗病中的作用.安徽农业科学,33(9):1705-1707
58.吕印谱,丁征宇,宋宝安.2007.抗植物病毒剂作用机制研究进展.中国植保导刊,27(10):14-16
59.马镝,赵秀香,吴元华.2008.壳寡糖对烟草花叶病毒的抑制作用及其对烟草酶活性的影响.中国生物防治,24(2)154-158
60.马志卿,殷培军,郭志波.2007.植物源病毒抑制剂VFB诱导烟草抗烟草花叶病毒机理的初步研究.植物病理学报,37(3):296-300
61.苗洪芹.2005.植物病毒检测技术和防治策略.河北农业科学,119(13):103-106
62.慕康国,赵秀琴,李健强.2005.矿质营养与植物病害关系研究进展.中国农业大学学报,5(1):84-90
63.年夫照,胡承孝,徐芳森,2008,硼对不同硼效率甘蓝型油菜叶片 6-磷酸葡萄糖脱氢酶活性和可溶性糖含量的影响,云南农业大学学报,23(11):47-51
64.宁淑萍,黄慧润,王小兰.2005.褐飞虱取食诱导的MAPK3基因在水稻叶鞘组织中的定位.热带亚热带植物学报,13(5):381-385
65.彭金英,黄勇平.2005.植物防御反应的两种信号转导途径及其相互作用.植物生理与分子生物学学报,31(4):347-353
66.秦碧霞,蔡健和,周兴华.2008.几种药剂防治烟草花叶病毒病田间试验.广西农业科学,39(1):37-39
67.任彩虹,张丽萍,闫桂琴.2007.高温胁迫对马铃薯幼苗抗氧化酶系统和叶绿素含量的影响.科技情报开发与经济,17(14):181-183
68.商闯,马春红,翟彩霞.2007.丙二醛(MDA)含量在玉米诱导抗病过程中的变化.华北农学报,22(增刊):29-32
69.商文静,吴云锋,商鸿生.2008.壳寡糖对烟草花叶病毒的体外钝化作用.病毒学报,24(1):76-78
70.尚庆茂,张志刚,宋士清.2007.壳聚糖诱导黄瓜幼苗抗灰霉病.植物保护学报,34(2):136-140
71.石杰,张磊,张琴.2008.接种耐酸根瘤菌和施钙对酸性土上紫花苜蓿生长的影响.植物营养与肥料学报,14(3):602-607
72.石磊,杨玉华,徐芳森.2002.硼对作物细胞膜功能影响的研究进展,21(4):395-400
73.石延霞,李宝聚,刘学敏.2007.高温诱导黄瓜抗霜霉病机理.应用生态学报.18(2):389-394
74.石延霞,张楠,李宝聚.2008.细菌性角斑病病菌诱导黄瓜产生系统抗病性机理的研究.园艺学报,35(2):221-226
75.时忠杰,杜阿朋,胡哲森.2007.水分胁迫对板栗幼苗叶片活性氧代谢的影响.林业科学研究.20(5):683-687
76.史胜青,齐力旺,肖文发.2008.外源GABA对NaCl胁迫下中间锦鸡儿幼苗乙烯生成的调控作用.林业科学,144(19):26-30
77.宋东辉,宋凤鸣,郑重.2004.MAP激酶在植物信号传递网络中的功能.浙江大学学报,30(2):119-126
78.宋敏丽.2007.茄子嫁接抗黄萎病与叶片叶绿素含量、相对电导率和游离脯氨酸含量的关系.山西农业科学,35(4):51-53
79.孙彤,黄勤妮,印莉萍.2004.缺铁水稻根调控因子和信号转导相关转录本微点阵分析.生物信息 学,2(4):5-8,29
80.孙伟,焦奎.2002.酶联免疫吸附分析法在植物病毒检测中的应用.化学研究与应用,14(5):510-514
81.孙永林.2008.铅对黄瓜叶片丙二醛脯氨酸和可溶性总糖含量的影响.长江蔬菜,12b:28-30
82.唐海明,徐一兰,陈金湘.2007.植物脱落酸、多胺和乙烯与逆境的关系.作物研究,21(5):501-505
83.田兆丰,裘季燕,李永丹.2003.天然抗病毒组合制剂对柿椒病毒病防效试验.植物保护,29(4):33-35
84.王东,于振文.2008.不同施氮量下子粒灌浆不同阶段遮光对小麦氮素积累和转移的影响.植物营养与肥料学报,14(4):615-622
85.王冬梅,康立功,许向阳.2007.热胁迫对番茄叶片主要生理生化指标的影响.园艺学进展,898-901
86.王瑞,马凤鸣,李彩凤.2008.低温胁迫对玉米幼苗脯氨酸、丙二醛含量及电导率的影响.东北农业大学学报,39(5):20-23
87.王少先,刘保国,郭秀璞.1998.病毒对番茄花药中游离脯氨酸含量及花粉萌发率的影响.落雁农专学报.18(3):14-16
88.王媛,杨红玉.2006.植物的抗病性及其分子机制.安徽农学通报,12(9):47-50
89.魏源文,胡春锦,李杨瑞.2006.乙烯在植物应答水分胁迫和病原菌浸染中的作用.植物生理学通讯,42(5):981-9851
90.文才艺,吴元华,张艳红.2000.烟草感染马铃薯Y病毒脉坏死株系后PPO活性及其同工酶变化的研究.华中农业大学学报,19(4):328-330
91.文才艺,吴元华,张艳红.2001.过氧化物酶活性与烟草脉坏死病抗性的关系.华中师范大学学报,35(3):334-337
92.吴涛,宗晓娟,谷令坤.2006.植物中的MAPK及其在信号传导中的作用.生物技术通报,5:1-7
93.吴元华,钟丽娟,赵秀香.2007.烟草感染PVY~N后叶脉坏死与总酚类黄酮及PPO关系研究,37(4):398-402
94.徐爱春,李保国,齐国辉.2003.苹果矿质营养研究进展.河北林果研究,18(4):368-375
95.杨洪强,接玉玲.2003.植物MAPK及其在病原信号传递中的作用.植物病理学报,33(1):8-13
96.杨辉,沈火林,朱鑫.2006.防御酶活性、木质素和总酚含量与辣椒抗黄瓜花叶病毒的关系.中国农学通报,22(5):369-373
97.杨秀清,闫海冰,李国明.2005.铜锌元素与仁用杏丙二醛含量关系的研究.山西林业科技,2005(4):4-6
98.殷培军,马志卿,冯俊涛.2005.VFB诱导烟草叶片生理生化变化与TMV抗性的关系.西北农业学报,14(2):91-95
99.尹立红,马志卿,陈安良.2003.矿质元素与植物抗病虫草害关系研究进展.西北农林科技大学学报,31(Suppl):158-161
100.印芳,葛红,彭克勤.2006.酚类物质与蝴蝶兰褐变关系初探.园艺学报.33(5):1137-114
101.于志章,王熙丽,永刘平.1993.黄瓜种子乙烯释放量酶活性与品种性型的关系.西北农业大学学报,21(4):77-80
102.余朝阁,李天来,杜妍妍.2007.植物诱导抗病性及其信号转导途径.北方园艺,7:73-76
103.余潮,唐伟中,涂志辉.2000.植物抗病过程中的活性氧代谢(一)--植物抗病过程中活性氧的产生及相关酶.江西植保,23(3):36-46
104.余潮,朱友林,葛刚.2000.植物抗病过程中的活性氧代谢(二)--植物抗病过程中活性氧的作用与引起活性氧产生的信号传导.江西植保,23(3)49.59.93
105.余迪求,陈利钢,张利平.2006.转录调控因子WRKY超级家族:起源、结构和功能.云南植物研究,28(1):9-77
106.袁有波,李继新,丁福章.2008.干旱胁迫对烤烟叶片脯氨酸和可溶性蛋白质含量的影响.安徽农业科学,36(21):8891-889
107.曾任森,苏贻娟,叶茂.2008.植物的诱导抗性及生化机理.华南农业大学学报,29(2):1-6
108.张策,谢远红,罗云波.2008.VIGS诱导番茄果实LeETR4沉默及其对乙烯生成的影响.食品工业科技,29(3):125-130
109.张风娟.2006.光肩星天牛对槭树挥发物的响应及虫害诱导信号物质研究.北京林业大学
110.张付云,冯斌,杜昱光.2005.壳寡糖诱导的烟草SKP1基因表达.植物生理与分子生物学学报,31(2):213-216
111.张富平,张蕊.2007.低温下外源水杨酸对玉米幼苗保护酶活性的影响.玉米科学,15(4):83-85
112.张亚冰,刘崇怀,潘兴.2006.盐胁迫下不同耐盐性葡萄砧木丙二醛和脯氨酸含量的变化.河南农业科学,2006(4):84-86
113.张玉凤,齐军山,陈靠山.2006.钕诱导番茄产生抗性的时间研究.中国稀土学报,24:196-199
114.赵利辉,邱德文,刘峥.2006.植物SAR和ISR中的乙烯信号转导网络.生物技术通报,3:28-32
115.赵茂俊,马永毅,吴明君.2006.植物抗病作用机制与信号转导研究进展.草业与畜牧,130:1-8
116.赵小明.2006.壳寡糖诱导植物抗病性及其诱抗机理的初步研究.中国科学院大连化学物理研究所
117.郑永华,席与芳,应铁进.1993.批把果实采后呼吸与乙烯释放规律的研究.园艺学报,20(2):111-115
118.郑昭英,孙小镭,刘世琦.2003.乙烯在黄瓜体内分布及周年变化动态研究初报.山东农业科学,4:15-17
119.钟丽娟,赵秀香,贾玉才.2006.烟草感染马铃薯Y病毒脉坏死株系后内源激素的变化.安徽农业科学,34(15):3724-3725
120.周功克,梁厚果.2000.盐胁迫下烟草愈伤组织内源活性氧及乙烯的产生与交替途径发生、运行的关系.实验生物学报,33(4):285-292
121.周利飞,刘映红,孙现超.2007.烟草芜菁花叶病毒的ELISA和RT-PCR快速检测技术研究.西南农业学报,20(4):758-760
122.周莎莎,周亚平,冯耀祖.2008.苹果原汁褐变有关因素的研究.青岛农业大学学报,25(2):81-83
123.周晓慧,JosephN,Wolukau.2007.甜瓜蔓枯病抗性与SOD、CAT和POD活性变化的关系.中国瓜菜,(2):4-6
124.朱春玉,吴元华,赵秀香.2007.嘧肽霉素对烟草花叶病毒在不同寄主上的防效研究.辽宁大学报,34(1):50-53
125.Abel R,IraidaA,VictorianoV,et al.ABA- and ethylene-mediated responses in osmotically stressed tomato are regulated by the TSS2 and TOS1 loci.Journal of Experimental Botany,57(12):3327-3335
126.Ada V,Michal H,Benjamin A.H,et al.2005.Trichoderma Mitogen-Activated Protein Kinase Signaling Is Involved in Induction of Plant Systemic Resistance.Applied and environmental microbiology,71(10):6241-6246
127.Agrawal G K,Rakwal R,Jwa N S,et al.2002.Molecular cloning and mRNA expression analysis of the first rice jasmonate biosynthetic pathway gene allene oxide synthase.Plant Physiology and Biochemistry,40:771 -782
128.Ajith A.,Srinivasa R.U.,Choong-M.R.,et al.2008.Salicylic Acid and Systemic Acquired Resistance Play a Role in Attenuating Crown Gall Disease Caused by 1[W][OA].Plant Physiol.146:703-715
129.Aleksandra W,Florina V,Caroline S,et al.2008.An Update on Abscisic Acid Signaling in Plants and More....Molecular Plant,1(2):198-217
130.Allan A C,Fluhr R.1997.Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells.The Plant Cell,9:1559-1572
131.Aloni R,Wolf A,Feigenbaum P,Avni A,Klee HJ.1998.The never ripe mutant provides evidence that tumor-induced ethylene controls the morphogenesis of Agrobacterium tumefaciens- induced crown galls in tomato stems.Plant Physiol,117:841-849
132.Alvarez M,Pennell R I,Vbijer PJ et al.1998.Reactive oxygen intermediates mediate a systemic signal net work in the establishment of plant immunity.Cell,92:773-784
133.Amano M,Toyoday K,Ichimosey et al.1997.Association between ion fluxes and defense responses in pea and cowpea tissues.Plant Cell Physiol,38(6):698 -706
134.AN Yu.SHEN Ying-bai.WU Li-juan.2006.A change of phenolic acids content in poplar leaves induced by methyl salicylate and methyl jasmonate.Journal of Forestry Research.17(2):107-110
135.Andrea A.L,Hiromasa Sa,Georg F,et al.2005.Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants.PNAS,102(30):10736-10741
136.Andrew J.H,Xinnian D.2006.Fitness Benefits of Systemic Acquired Resistance During Hyaloperonospora parasitica Infection in Arabidopsis thaliana Copyright,173:1621-1628
137.Androulla Gilliland .Davinder P.Singh .Jennifer M.Hayward.et al.2003.Genetic Modification of Alternative Respiration Has Differential Effects on Antimycin A-Induced versus Salicylic Acid-Induced Resistance to Tobacco mosaic virus.Plant Physiol.132:1518-1528
138.Ange'lique B.,Ce'cile C.,Adrien G.,et al.2008.Nitric Oxide in Plants:Production and Cross-talk with Ca~(2+) Signaling.Molecular Plant 1(2):218-228
139.B.KASSANIS,R.F.WHITE.1974.Inhibition of Acquired Resistance to Tobacco Mosaic Virus by Actinomycin D.Great Britain.25:323-324
140.Balachandran S,Osmond C B,Daley P F.1994.Diaynos is of the earliest stra-specific interactions between tobacco mosaic virus and chlorop lastes of tobacco leaves in vivo by means of chlorophyll fluorescence imaging [J].Plant Physiology,104(3):1059 -1065
141.Brad MB,Ronan C.O'M,Wuyi W,et al.2006.Ethylene Stimulates Nutations That Are Dependent on the ETR1 Receptor.Plant Physiology,142:1690-1700
142.Changhua Z,Lijun G,Zhenguo S,et al.2006.Interactions between jasmonates and ethylene in the regulation of root hair development in Arabidopsis.Journal of Experimental Botany,57(6):1299-1308
143.Chen KM,Gong HJ,Chen GC,Zhang CL.2002.ACC and MACC biosynthesis and ethylene production in water-stressed spring wheat.Acta Bot Sin,44(7):775-781
144.Chen N,Goodwin PH,Hsiang T.2003.The role of ethylene during the infection of Nicotiana tabacum by Colletotrichum destructivum.J Exp Bot,54(392):2449-2456
145.Creeiman R A.1992.Jasmonic acid/ methy jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression.Proc.Natl.Acad.Sci,89:4938—4941
146.Cristinzo G,Scala F.1994.Electrolyte leakage as a potential method for measuring of eggplant resistance and V.dahliae Virulence.Phytopathology,29(4):184 -190
147.Dean J F D,Gamble H R,Andersom J D.1989.The ethylene Biosynthesis- inducing xyganase:its induction in Trichoderma viride and certain plant pathogens [J].Phytopathology,79:1071-1078.
148.Dean R.A.,Kuc J.1986.Induced systemic protection in cucumber:time of production and movement of the signal.Phytopathology,76(10):966-970
149.Dean R.A.,Kuc J.1987.Rapid lignification in response to wounding and infection as a mechanism for induced systemic protection in cucumber.Physiological and Molecular Plant Phytopathology.31:69-81
150.Dempsey D,Shah J,Klessing D F.1999.Salicylic acid and disease resistance in plant [J].Critical Reviews in plant science,18(6):547-575
151.Dixon R A,Harrison M J,Lamb C J.1994.Early events in the activation of plant defense responses.Annu Rev Phytopathology.32:479-486
152.Doke N.1983.Generation of super oxide anion by potato tuber protoplasts upon the hypersensitive response to hyphal wall components of phytophthora infestans and specific inhibition of the reaction by suppressors of hypersensitive [J].Plant Physiology,23:359-367.
153.Dong H P,Peng J L,Bao Z L,et al.2004.Downstream divergence of the ethylene signaling pathway for harpin-stimulated Arabidopsis growth and insect defense.Plant Physiol,136:3628-3638
154.Dongtao R ,Kwang-YY ,Guo-JL,et al.2006.Activation of Ntf4,a Tobacco Mitogen-Activated Protein Kinase,during Plant Defense Response and Its Involvement in Hypersensitive Response-Like Cell Death.Plant Physiology,141:1482-1493
155.Droillard M J,Gudu J,Le Caer J P,et al.1997.Indentification of calreticulin like protein as one of the phosphoproteins,modulated in response to oligo galacturonides in tobacco ceddls.Planta,202(3):341-348
156.Durner J,Shah LKlessig D H.1997.Salicylic acid and disease resistance in plants.Trends Plant Sci,2:266-274
157.Durner J,Wendohenne D,Klessing D F.1997.Salicylic acid and disease resistance in plants.Trends Plant Sci,2:266-274
158.Durner J,Wendohenne D,Klessing D F.1998.Defense gene induction in tobacco by nitricoxide,cyclic ADP-ribose.Proc Natl Acad Sci USA,95:10328-10333
159.Ecker JR.1995.The ethylene signal transduction pathway in plants.Science,268(5):667-665
160.El-Sharkawy,W.S.K,A.E-K,et al.Isolation and characterization of four ethylene signal transduction elements in plums {Prunus salicina L.).ournal of Experimental Botany,58(13):3631-3643
161.Fawe A,Abou-Zaid M.1998.Silic on-mediated accumulation of flavonoid phytoalexins in cucumber.Phytopathology,88(5):396-401
162.Felix G,Regenass M,Boiler T.1993.Specific perception of subnanomolar concentrations of chitin fragments by tomato cells.Induction of extra cellular alkalinization changes in protein phosphorylation and establishment of a refractory state.Plant J,4(2):307-316
163.Fidanza M A,Dernoeden P H.1996.Interaction of potassium source application timing and fungicide on Rhizoctonia blight in ryegrass [J].Hort Sci,19(3):389-392.
164.Fuminor iT,Riichiro Y,Kazuya I,et al.2007.The Mitogen-Activated Protein Kinase Cascade MKK3-MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in Arabidopsis.ThePlantCell,19:805-818
165.Glenn E.B,Betty K.I.2007.Ethylene-sensitive and insensitive regulation of transcription factor expression during in vitro tomato sepal ripening.Journal of Experimental Botany,58(8):2043-2051
166.Godfrey P.M,MarcusA.S,Alan M.J,et al.2004.Mastoparan Rapidly Activates Plant MAP Kinase Signaling Independent of Heterotrimeric G Proteins.Plant Physiology,134:1332-1336
167.G(?)mez-Cadenas A,Tadeo F R,Primo-Millo E,et al.1998.Pathogen-induced systemic activation
168.Gundlach H,Muller M J,Kutchan T M,et al.1992.Jasmonic acid is a signal transducer in elicitor induced plant cellc ultures.Proc Natl Aced Sci USA,89:2389-2393
169.Hanhong B,Moon SK,Richard CS,et al.2006.Necrosis- and Ethylene-Inducing Peptide from Fusarium oxysporum Induces a Complex Cascade of Transcripts Associated with Signal Transduction and Cell Death in Arabidopsis.Plant Physiology,141:1056-1067
170.HELENA BIRECKA,JAMES L.CATALFAMO.PAUL URBAN.1975.Cell Wall and Protoplast Isoperoxidases in Tobacco Plants in Relation to Mechanical Injury and Infection with Tobacco Mosaic Virus.Pant Physiol.(1975) 55:611-619
171.Ingo H,Maria B-P,Katarina H,et al.2005.Virus-Induced Gene Silencing-Based Functional Characterization of Genes Associated with Powdery Mildew Resistance in Barley.Plant Physiology,138:2155-2164
172.Javier A,Paz M,Manuel C,et al.2008.Ethylene-induced differential gene expression during abscission of citrus leaves.Journal of Experimental Botany,59(10):2717-2733
173.Justin L,Daniel FK,Thorsten N.2001.A Harpin Binding Site in Tobacco Plasma Membranes Mediates Activation of the Pathogenesis-Related Gene HIN1 Independent of Extracellular Calcium but Dependent on Mitogen-Activated Protein Kinase Activity.The Plant Cell,13:1079-1093
174.Kaoru S,Akira Y,Hideaki S.1999.Slow and Prolonged Activation of the p47 Protein Kinase during Hypersensitive Cell Death in a Culture of Tobacco Cells.Plant Physiology,19:1465-1472
175.Kerry F.P,Gregory B.M.2004.Identification of MAPKs and Their Possible MAPK Kinase Activators Involved in the Pto-mediated Defense Response of Tomato.THE JOURNAL OF BIOLOGICAL CHEMISTRY,279(47):49229-49235
176.Klarzynski O,Descamps V,Plesse B,et al.2003.Sulfated fucan oligosaccharides elicit defense responses in tobacco and local and systemic resistance against tobacco mosaic virus.Molecular plant-Microbe Interaction,16(2):115-122
177.Klarzynski O,Plesse B,Joubert J M,et al.2000.Linear B-1,3 Glucans are elicitors of defense responses in tobacco.Plant Physiol,124(3):1027-1038
178.Klessing D F,Durner J,Noad R,et al.2000.Nitric oxide and salicylic acid signaling in plant defense.Proc Natl Acad Sci USA.,97:8849-8855
179.Klessing D F,Malamy J.1994.The salicylic acid signal in plants.Plant Mol Bind,26:1439-1458.
180.Kuchitsu K,Kikuyama M,Shibuya N.1993.N-acetyl-chito oligo saccharides biotic elicitor for phytoalexin production,induce transient membrane depolarization in suspension-cultured rice cell.Protoplasma,174(1-2):79-81
181.Kurosaki F,Tsurusawa Y,Nishi A.1987.Break down of phosphatidylinositol during the elicitation of phytoalex in production in cultured carrot cells.Plant Physiol,85:601
182.Kurstak E.1981.Handbook of plant virus infections and comparative diagnosis [M],Elsevier/North-Holland:Biomedical Press,258-277.
183.Kwang-Yeol Y,Yidong L,Shuqun Z.2001.Activation of a mitogen-activated protein kinase pathway is involved in disease resistance in tobacco.PNAS,98(2):741-746
184.Linda M.R,Jessica S.W,Matthew J.C,et al.2007.Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response,including ERF1 induction.Journal of Experimental Botany,58(10):2627-2639
185.Livio T,Alice T,Giorgio C.2007.The involvement of auxin in the ripening of climacteric fruits comes of age:the hormone plays a role of its own and has an intense interplay with ethylene in ripening peaches.Journal of Experimental Botany,58(12):3299-3308
186.Malamy J,Carr J P,Klessig D F,et al.1990.Salicylic acid:a likely endogenous signal in the resistance response of tobacco to viral infection.Science,250:1002-1004
187.Marcus A.S,Ankit W,Shawn D.M,et al.2005.Overexpression of SIPK in tobacco enhances ozone-induced ethylene formation and blocks ozone-induced SA accumulation.Journal of Experimental Botany,56(418):2195-2201
188.Masanori T,John LF,Elizabeth A.H.P-S.2008.Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in ArabidopsisPlant.Physiology,146:1219-1230
189.Matsuda F,Miyagawa H,Ueno T.2001.Involvement of reactive oxygen species in the induction of(S)-N-p-coumaroyloctopamine accumulation by beta-l,3-glucooligosaccharide elicitors in potato tuber tissues.Z Natu rforsch,56(3-4):228-234
190.Mayer M G,et al.1988.An elicitor from Phytophthora megasperma f.sp.glycinea influences the membrane potential of soybean cotyledonar cell.Physiological and Molecular Plant Pathology,33:397-407
191.Mitter,Neena,Kazan,et al.1998.Systemic induction of an Arabidopsis plant defensin gene promoter by tobacco mosaic virus and jasmonic acid in transgenic tobacco.Plant Science,136(2):169-180
192.NING Jing.YUAN Bin.XIE Ka-Bin.et al.2006.Isolation and Identification of SA and JA Inducible Protein Kinase Gene OsSJMKl in Rice.Acta Genetica Sinica.33(7):625-633
193.Ornella C,Nathalie G,Catherine B,et al.2001.A Novel Tobacco Mitogen-activated Protein(MAP) Kinase Kinase,NtMEKl,Activates the Cell Cycle-regulated p43~(Ntf6) MAP Kinase.THE JOURNAL OF BIOLOGICAL CHEMISTRY,276(21):18139-18145
194.Orozco-Cardenas M L,Ryan C A.2002.Nitric oxide negatively modulates wound signaling in tomato plants.Plant Physiol,130(1):487-493
195.Peng M,Kuc J.1992.Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks [J].Phytopathology,82:718-722.
196.Penninckx IAMA,Thomma BPHJ,Buchala A,et al.1998.Concomitant activation of jasmonate
197.Philippe R,Auzanne G,Kalanethee P,et al.1995.Oligogalacturonide defense signal in plants:Large fragments interact with the plasma membrane in vitro.Proc Natl Acad Sci USA,92:4145-4149
198.Revmond P,Farmer E E.1998.Jasmonate and salicylate as global signals for defense gene expression.Current Opinion in Plant Biology,1(5):404-411
199.Reymond P,Grunberger S,Paul K,Muller M.1995.Oligosaccharide defence signals in plants:largefrgmants interact with the plasmam embrane in vitro.Froc Nail Acad Sci USA,92:4145-4149
200.Robert J.S,Ellen N.F,Edwige J.F.S,et al.2007.A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway.Plant Physiology,144:1899-1912
201.Schaller F.2001.Enzymes of the biosynthesis of octadecanoide-derived signaling moleculars Journal of Experimental Botany,52:11-23
202.Shuqun Z,Daniel F.Klessig.1997.Salicylic Acid Activates a 48-kD MAP Kinase in Tobacco.The Plant Cell,9:809-824
203.Shuqun Z,Daniel F.K.1998.Resistance gene N-mediated de novo synthesis and activation of a obacco mitogen-activated protein kinase by tobacco mosaic irusinfection.Proc.Natl.Acad.Sci.USA,95:433-7438
204.Shuqun Z,He D,Daniel F.Klessig.1998.Activation of the Tobacco SIP Kinase by Both a Cell Wall-Derived Carbohydrate Elicitor and Purified Proteinaceous Elicitins from Phytophthora spp.The Plant Cell,10:425-449
205.Shuqun Z,Yidong L.2001.Activation of Salicylic Acid-Induced Protein Kinase,a Mitogen-Activated Protein Kinase,Induces Multiple Defense Responses in Tobacco.The Plant Cell,13:1877-1889
206.ShutaAsai.K.,Hirofumi Y.2008.MAPK Signaling Regulates Nitric Oxide.and NADPH Oxidase- Dependent Oxidative Bursts in WOA Nicotiana benthamiana.American Society of Plant Biologists.20:1390-1406
207.Simone F,Roberta G,Carine D,et al.2007.Resistance to Botrytis cinerea Induced in Arabidopsis by Elicitors Is Independent of Salicylic Acid,Ethylene,or Jasmonate signaling but requires PHYTOALEXIN DEFICIENT3.Plant Physiol,144:367-379
208.Simpson S D,Ashford D A,Jarvey D J,et al.1998.Short chain oligogalacturobides induce ethylene production and expression of the gene encoding aminocvclopropane 1-carboxvlic acid oxides in tomato plants.Glvcobiol,8(6):579-583
209.Sjoerd VE,Bas WMV,Ronald VD,et al.2008.MYB72 Is Required in Early Signaling Steps of Rhizobacteria-Induced Systemic Resistance in Arabidopsis.Plant Physiology,146:1293-1304
210.Solomos T,Laties G G.1976.Ef fect of cyanide and ethylene on the respirarion of cyanide sensitive and cyanide-resistant plant tissues.Plant Physiol,58:47-50
211.Song F,Goodman R M.2001.Activity of nitric oxide is dependent on,but is partially required for function of salicylic acid in the signaling pathway in tobacco systemic acquired resistance.Mol Plant Microbe Interact,14(12):1458-1462
212.SONG Zhen-xia.GAO Ming.WANGLi-ao,et al.2008.Effects of Boron Fertilizer Application Rates on the Yieldand Quality of Flue2Cured Tobacco.Journal of Southwest University.30(6):64-72
213.Strasser H,Hottman C,Grisebach H,et al.1986.Are polyphosphoinositides involved in signal transduction of elicitor induced phytoalexin synthesis in cultured plant cell ? Z Naturforsch,41:717
214.Tang D,Christiansen KM,Innes RW.2005.Regulation of plant disease resistance,stress responses,cell death,and ethylene signaling in Arabidopsis by the EDR1 protein kinase.Plant Physiol,138:1018-1026
215.Taro O,Ling P,Maki K-Y,et al.2005.Functional Analysis of Arabidopsis Ethylene-Responsive Element Binding Protein Conferring Resistance to Bax and Abiotic Stress-Induced Plant Cell Death.Plant Physiology,138:1436-1445
216.Tatiana EM,Jurgen Z.2006.The Arabidopsis Flavin-Dependent Monooxygenase FMO1 Is an Essential Component of Biologically Induced Systemic Acquired Resistance.Plant Physiology,141:1666-1675
217.teven R.S,Li H,Amanda S.B,et al.2005.Development of a Virus-Induced Gene-Silencing System for Hexaploid Wheat and Its Use in Functional Analysis of the Lr21-Mediated Leaf Rust Resistance Pathway.Plant Physiology,138:2165-2173
218.Thomas P,Ame'rin V,Brad M.B,et al.2006.The Exoribonuclease XRN4 Is a Component of the Ethylene Response Pathway in Arabidopsis.The Plant Cell,18:3047-3057
219.Tina R,Pedro P,Shuqun Z,et al.1999.Rapid Avr9- and Cf-9-Dependent Activation of MAP Kinases in Tobacco Cell Cultures and Leaves:Convergence of Resistance Gene,Elicitor,Wound,and Salicylate Responses.The Plant Cell,11:273-287
220.Tsuyoshi Mi,Kenji,Iriet,et al.1996.A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch,cold,and water stress in Arabidopsis thaliana.Proc.Natl.Acad.Sci.USA,93:765-769
221.Vranova E,Inze D,Breusegem FV.2002.Signal transdution during oxidative stress.J Exp Bot,53:1227-1236.
222.Walter E,Riedell,Shannon L.2007.Crop mineral nutrient and yield responses to aphids or barley yellow dwarf virus in spring wheat and oat [J].Crop Science,47:1553-1560.
223.Warington K.1923.The effect of boric acid and borax on the broad bean and certain other plants .Ann Bot,37:629-672.
224.Yang et al.1997.Signal perception and transduction in plant defense response.Genes Dev,1621-1639.
225.Yi-Feng C,Naomi E,G.Eric Schaller.2005.Ethylene Signal Transduction.Annals of Botany,95:901-915
2.陈芳,慕小倩,梁宗锁.2007.水杨酸对附子叶斑病的诱导抗性及作用机理研.西北农业学报,16(2):245-249
3.陈力力,高必达,2006.微生物抗植物病毒活性物的研究进展,中国生物防治,22(4)255-260
4.陈齐斌,沈嘉祥.2005.抗植物病毒剂研究进展和面临的挑战与机遇.云南农业大学学报,20(4):505-511
5.陈娅斐,冯斌,赵小明.2005.MAPK级联途径在植物信号转导中的研究进展.植物学通报,22(3):357-365
6.陈英,黄敏仁,诸葛强.2002.植物抗病信号传导途径及其相互作用.南京林业大学学报,26(3):85-90
7.程伟东,江禹奉,谭贤杰.2008,水杨酸诱导玉米纹枯病系统抗性的初步研究.广西农业科学,39(6):785-787
8.崔婧.2007.水杨酸与植物抗逆性.安徽农学通报,13(9):35-38
9.单耀军,张进霞,袁洪水.2006.氨基酸铜对6种植物病原真菌生长的抑制作用.河北大学学报,26(1):30-37
10.邓文红,张风娟,金幼菊.2007.反相高效液相色谱法测定植物组织中的水杨酸.北京林业大学学报,29(1):151-154
11.董涛,张志刚,尚庆茂.2009.水杨酸和壳聚糖对黄瓜幼苗耐盐性的共诱导作用.中国蔬菜,2009(2):46-50
12.董英,胡冠娟.2006.壳聚糖对板栗褐变的抑制作用.江苏农业科学2006(4):133-135
13.杜新民,张永清.2008.施锌对石灰性褐土上小白菜光合作用及保护酶活性的影响.西北植物学报.28(6):1203-1207
14.范海延.2003.葡聚寡糖诱导蔬菜抗病性的研究.沈阳农业大学
15.冯东昕,李宝栋.1998.可溶性硅在植物抵御病害中的作用.植物病理学报.28(4):293-297
16.冯高,刘华.2008.温度和光照对PVY生物测定的影响.中国马铃薯,18(3):145-146
17.傅晓艺,刘桂茹,杨学举.2005.植物病害中的信号传导.安徽农业科学,33(5):901-903
18.关洪丹,姜新,李晔.2006.不同供铁水平对烟草与PVY~N互作关系中防御酶活性的影响.安徽农业科学.34(15):3722-3723
19.郭红莲,李丹,白雪芳.2002.壳寡糖对烟草病毒的诱导抗性研究.中国烟草科学,4:1-3
20.郭红莲.2003.壳寡糖诱导植物抗病机理及其信号转导的研究.中国科学院大连化学物理研究所.1-85
21.郭红莲等,2003.壳寡糖对棉花悬浮培养细胞活性氧的作用.中国海洋药物,(1):11-13
22.郭丽,段金秀,梁占训.2007.杜氏盐藻MAPK基因cDNA的克隆及其进化分析.草业科学,24(1):21-25
23.郭丽红,王定康,杨晓虹,陈善娜.2004.外源乙烯利对干旱胁迫过程中玉米幼苗某些抗逆生理指标的影响.云南大学学报(自然科学版),6(4):352-356
24.郭兴启,吕士恩,朱汉城.1999.烟草上马铃薯Y病毒的提纯与检测.中国烟草科学,1999(2):5-7
25.郭兴启,朱汉城,吕士恩.1998.酶标记羊抗兔间接法检测烟草马铃薯Y病毒的研究.中国烟草报,4(2):38-42
26.郭衍银,徐坤,王秀峰.2003.矿质营养与植物病害机理研究进展.甘肃农业大学学报,38(4):385-393
27.何开跃,李晓储,黄利斌.2000.冷冻胁迫对福建柏苗可溶性糖和丙二醛(MDA)含量的影响.江苏林业科技,27(6):6-8
28.黄芳,王建明,徐玉梅.2008.硼抑制灰霉病菌孢子萌发机制的初步研究.植物病理学报,38(4):370-376
29.黄国存,张继澎,孙大业.1993.对采后番茄CAM呼吸强度及乙烯产生的影响.园艺学报,20(4):403-404
30.纪秀娥,张美善,于海秋.1998.植物的硅素营养.农业与技术,1998(4):11-13
31.姜国勇,杨仁崔.2005.番茄花叶病毒移动蛋白和番茄抗病基因Tm-22的互作诱导烟草转化体程序性细胞死亡.微生物学报,45(2):301-304
32.姜华,宁淑香,杨文新.2002.壳聚糖对MDMV的防效与玉米叶片防御酶活性的关系.辽宁师范大学学报,25(1):72-75
33.李宝聚,范海延,孙艳秋.2005.葡聚六糖诱导黄瓜体内水杨酸的积累及其与抗霜霉病关系的初步研究.园艺学报,32(1):115-117
34.李怀方,张莉.钙、镁离子对烟草花叶病毒侵染的抑制作用.植物保护.20(5):2-4
35.李淮,王莱,武国凡.2006.植物抗病性的分子机制和信号传导.西北师范大学学报,46(2):113-117
36.李金玉,李冠,赵惠新.2006.植物抗病分子机制研究进展.种子,25(2):45-50
37.李金柱,吴礼树,杨玉华.2004.硼在植物细胞壁上营养机理的研究进展.中国油料作物学报,26(4):96-99
38.李娟.2007.植物钾、钙、镁素营养的研究进展.福建稻麦科技,25(1):39-42
39.李莉,任金平,曲柏宏.2007.水分胁迫对苹果梨叶片可溶性糖、脯氨酸含量的影响.吉林农业科学,32(1):51-54
40.李宁,樊守金,张增艳.2007.植物抗病相关启动子及其研究进展.植物遗传资源学报,8(2):234-239
41.李群.2005.钙依赖蛋白激酶和腺苷酸环化酶参与拟南芥响应大丽轮枝菌毒诱导信号转导过程的实验证据.中国农业大学.1-70
42.李锐,李生泉,范月仙.低温胁迫对棉花幼苗SOD、CAT活性的影响.山西农业大学,18-19
43.李艳莉,吴晓华,马耀荣.2006.反相高效液相色谱测定足光粉中苯甲酸、水杨酸的含量.佛山科学技术学院学报,24(4):68-71
44.李章海,刘登乾,田必文.2008.打顶和施用NAA对烟株生长、烟碱合成和钾素吸收的影响.烟草农学,254(9):52-55
45.李智辉,王新颖,周广柱.2007.低温胁迫下新铁炮百合幼苗叶片活性氧的产生及保护酶活性的变化.北方园艺,2007(12):106-108
46.梁和,马国瑞,石伟勇.2000.钙硼营养与果实生理及耐贮性研究进展.土壤通报,31(4):187-190
47.刘爱新,梁元存,张博.1998.植物诱导抗病性研究进展.山东农业大学学报,29(3):410-414
48.刘碧容,甄畅迪,萧洪东.2008.硼对草坪草超氧化物歧化酶活性、超氧阴离子产生速率和丙二醛含量的影响.华中农业大学学报.27(3):378-381
49.刘传和,刘岩,易干军.2008.地膜覆盖对菠萝植株有关生理指标的影响.热带作物学报,29(5):546-550
50.刘国强,吴锦程,唐朝晖.2008.枇杷采后酚类物质代谢与果肉木质化的关系.中国农学通报,24(1):247-251
51.刘强,张勇,陈受宜.2000.干旱 高盐及低温诱导的植物蛋白激酶基因.科学通报,45(6):561-566
52.刘世彪,易萍,罗奥.2008.低温胁迫对绞股蓝和五柱绞股蓝抗寒性生理指标的影响.热带作物学报,29(5):572-576
53.刘树玲.2006.稻瘟菌侵染过程中的信号传导.作物研究,2:194-198
54.刘太国,石延霞,文景芝.2003.水杨酸诱导烟草对TMV的抗性和PAL活性变化研究.植物病理学报,33(2):190-191
55.刘伟曲凌慧,刘洪庆.2008.温胁迫对葡萄保护酶和氧自由基的影响.北方园艺,(5):21-24
56.刘亚光,李海英,杨庆凯.2004.乙烯利对大豆抗性生化物质的诱导作用.哈尔滨工业大学学报,36(8):1014-1016
57.刘志文,沙爱华,王英.2005.活性氧物质在植物抗病中的作用.安徽农业科学,33(9):1705-1707
58.吕印谱,丁征宇,宋宝安.2007.抗植物病毒剂作用机制研究进展.中国植保导刊,27(10):14-16
59.马镝,赵秀香,吴元华.2008.壳寡糖对烟草花叶病毒的抑制作用及其对烟草酶活性的影响.中国生物防治,24(2)154-158
60.马志卿,殷培军,郭志波.2007.植物源病毒抑制剂VFB诱导烟草抗烟草花叶病毒机理的初步研究.植物病理学报,37(3):296-300
61.苗洪芹.2005.植物病毒检测技术和防治策略.河北农业科学,119(13):103-106
62.慕康国,赵秀琴,李健强.2005.矿质营养与植物病害关系研究进展.中国农业大学学报,5(1):84-90
63.年夫照,胡承孝,徐芳森,2008,硼对不同硼效率甘蓝型油菜叶片 6-磷酸葡萄糖脱氢酶活性和可溶性糖含量的影响,云南农业大学学报,23(11):47-51
64.宁淑萍,黄慧润,王小兰.2005.褐飞虱取食诱导的MAPK3基因在水稻叶鞘组织中的定位.热带亚热带植物学报,13(5):381-385
65.彭金英,黄勇平.2005.植物防御反应的两种信号转导途径及其相互作用.植物生理与分子生物学学报,31(4):347-353
66.秦碧霞,蔡健和,周兴华.2008.几种药剂防治烟草花叶病毒病田间试验.广西农业科学,39(1):37-39
67.任彩虹,张丽萍,闫桂琴.2007.高温胁迫对马铃薯幼苗抗氧化酶系统和叶绿素含量的影响.科技情报开发与经济,17(14):181-183
68.商闯,马春红,翟彩霞.2007.丙二醛(MDA)含量在玉米诱导抗病过程中的变化.华北农学报,22(增刊):29-32
69.商文静,吴云锋,商鸿生.2008.壳寡糖对烟草花叶病毒的体外钝化作用.病毒学报,24(1):76-78
70.尚庆茂,张志刚,宋士清.2007.壳聚糖诱导黄瓜幼苗抗灰霉病.植物保护学报,34(2):136-140
71.石杰,张磊,张琴.2008.接种耐酸根瘤菌和施钙对酸性土上紫花苜蓿生长的影响.植物营养与肥料学报,14(3):602-607
72.石磊,杨玉华,徐芳森.2002.硼对作物细胞膜功能影响的研究进展,21(4):395-400
73.石延霞,李宝聚,刘学敏.2007.高温诱导黄瓜抗霜霉病机理.应用生态学报.18(2):389-394
74.石延霞,张楠,李宝聚.2008.细菌性角斑病病菌诱导黄瓜产生系统抗病性机理的研究.园艺学报,35(2):221-226
75.时忠杰,杜阿朋,胡哲森.2007.水分胁迫对板栗幼苗叶片活性氧代谢的影响.林业科学研究.20(5):683-687
76.史胜青,齐力旺,肖文发.2008.外源GABA对NaCl胁迫下中间锦鸡儿幼苗乙烯生成的调控作用.林业科学,144(19):26-30
77.宋东辉,宋凤鸣,郑重.2004.MAP激酶在植物信号传递网络中的功能.浙江大学学报,30(2):119-126
78.宋敏丽.2007.茄子嫁接抗黄萎病与叶片叶绿素含量、相对电导率和游离脯氨酸含量的关系.山西农业科学,35(4):51-53
79.孙彤,黄勤妮,印莉萍.2004.缺铁水稻根调控因子和信号转导相关转录本微点阵分析.生物信息 学,2(4):5-8,29
80.孙伟,焦奎.2002.酶联免疫吸附分析法在植物病毒检测中的应用.化学研究与应用,14(5):510-514
81.孙永林.2008.铅对黄瓜叶片丙二醛脯氨酸和可溶性总糖含量的影响.长江蔬菜,12b:28-30
82.唐海明,徐一兰,陈金湘.2007.植物脱落酸、多胺和乙烯与逆境的关系.作物研究,21(5):501-505
83.田兆丰,裘季燕,李永丹.2003.天然抗病毒组合制剂对柿椒病毒病防效试验.植物保护,29(4):33-35
84.王东,于振文.2008.不同施氮量下子粒灌浆不同阶段遮光对小麦氮素积累和转移的影响.植物营养与肥料学报,14(4):615-622
85.王冬梅,康立功,许向阳.2007.热胁迫对番茄叶片主要生理生化指标的影响.园艺学进展,898-901
86.王瑞,马凤鸣,李彩凤.2008.低温胁迫对玉米幼苗脯氨酸、丙二醛含量及电导率的影响.东北农业大学学报,39(5):20-23
87.王少先,刘保国,郭秀璞.1998.病毒对番茄花药中游离脯氨酸含量及花粉萌发率的影响.落雁农专学报.18(3):14-16
88.王媛,杨红玉.2006.植物的抗病性及其分子机制.安徽农学通报,12(9):47-50
89.魏源文,胡春锦,李杨瑞.2006.乙烯在植物应答水分胁迫和病原菌浸染中的作用.植物生理学通讯,42(5):981-9851
90.文才艺,吴元华,张艳红.2000.烟草感染马铃薯Y病毒脉坏死株系后PPO活性及其同工酶变化的研究.华中农业大学学报,19(4):328-330
91.文才艺,吴元华,张艳红.2001.过氧化物酶活性与烟草脉坏死病抗性的关系.华中师范大学学报,35(3):334-337
92.吴涛,宗晓娟,谷令坤.2006.植物中的MAPK及其在信号传导中的作用.生物技术通报,5:1-7
93.吴元华,钟丽娟,赵秀香.2007.烟草感染PVY~N后叶脉坏死与总酚类黄酮及PPO关系研究,37(4):398-402
94.徐爱春,李保国,齐国辉.2003.苹果矿质营养研究进展.河北林果研究,18(4):368-375
95.杨洪强,接玉玲.2003.植物MAPK及其在病原信号传递中的作用.植物病理学报,33(1):8-13
96.杨辉,沈火林,朱鑫.2006.防御酶活性、木质素和总酚含量与辣椒抗黄瓜花叶病毒的关系.中国农学通报,22(5):369-373
97.杨秀清,闫海冰,李国明.2005.铜锌元素与仁用杏丙二醛含量关系的研究.山西林业科技,2005(4):4-6
98.殷培军,马志卿,冯俊涛.2005.VFB诱导烟草叶片生理生化变化与TMV抗性的关系.西北农业学报,14(2):91-95
99.尹立红,马志卿,陈安良.2003.矿质元素与植物抗病虫草害关系研究进展.西北农林科技大学学报,31(Suppl):158-161
100.印芳,葛红,彭克勤.2006.酚类物质与蝴蝶兰褐变关系初探.园艺学报.33(5):1137-114
101.于志章,王熙丽,永刘平.1993.黄瓜种子乙烯释放量酶活性与品种性型的关系.西北农业大学学报,21(4):77-80
102.余朝阁,李天来,杜妍妍.2007.植物诱导抗病性及其信号转导途径.北方园艺,7:73-76
103.余潮,唐伟中,涂志辉.2000.植物抗病过程中的活性氧代谢(一)--植物抗病过程中活性氧的产生及相关酶.江西植保,23(3):36-46
104.余潮,朱友林,葛刚.2000.植物抗病过程中的活性氧代谢(二)--植物抗病过程中活性氧的作用与引起活性氧产生的信号传导.江西植保,23(3)49.59.93
105.余迪求,陈利钢,张利平.2006.转录调控因子WRKY超级家族:起源、结构和功能.云南植物研究,28(1):9-77
106.袁有波,李继新,丁福章.2008.干旱胁迫对烤烟叶片脯氨酸和可溶性蛋白质含量的影响.安徽农业科学,36(21):8891-889
107.曾任森,苏贻娟,叶茂.2008.植物的诱导抗性及生化机理.华南农业大学学报,29(2):1-6
108.张策,谢远红,罗云波.2008.VIGS诱导番茄果实LeETR4沉默及其对乙烯生成的影响.食品工业科技,29(3):125-130
109.张风娟.2006.光肩星天牛对槭树挥发物的响应及虫害诱导信号物质研究.北京林业大学
110.张付云,冯斌,杜昱光.2005.壳寡糖诱导的烟草SKP1基因表达.植物生理与分子生物学学报,31(2):213-216
111.张富平,张蕊.2007.低温下外源水杨酸对玉米幼苗保护酶活性的影响.玉米科学,15(4):83-85
112.张亚冰,刘崇怀,潘兴.2006.盐胁迫下不同耐盐性葡萄砧木丙二醛和脯氨酸含量的变化.河南农业科学,2006(4):84-86
113.张玉凤,齐军山,陈靠山.2006.钕诱导番茄产生抗性的时间研究.中国稀土学报,24:196-199
114.赵利辉,邱德文,刘峥.2006.植物SAR和ISR中的乙烯信号转导网络.生物技术通报,3:28-32
115.赵茂俊,马永毅,吴明君.2006.植物抗病作用机制与信号转导研究进展.草业与畜牧,130:1-8
116.赵小明.2006.壳寡糖诱导植物抗病性及其诱抗机理的初步研究.中国科学院大连化学物理研究所
117.郑永华,席与芳,应铁进.1993.批把果实采后呼吸与乙烯释放规律的研究.园艺学报,20(2):111-115
118.郑昭英,孙小镭,刘世琦.2003.乙烯在黄瓜体内分布及周年变化动态研究初报.山东农业科学,4:15-17
119.钟丽娟,赵秀香,贾玉才.2006.烟草感染马铃薯Y病毒脉坏死株系后内源激素的变化.安徽农业科学,34(15):3724-3725
120.周功克,梁厚果.2000.盐胁迫下烟草愈伤组织内源活性氧及乙烯的产生与交替途径发生、运行的关系.实验生物学报,33(4):285-292
121.周利飞,刘映红,孙现超.2007.烟草芜菁花叶病毒的ELISA和RT-PCR快速检测技术研究.西南农业学报,20(4):758-760
122.周莎莎,周亚平,冯耀祖.2008.苹果原汁褐变有关因素的研究.青岛农业大学学报,25(2):81-83
123.周晓慧,JosephN,Wolukau.2007.甜瓜蔓枯病抗性与SOD、CAT和POD活性变化的关系.中国瓜菜,(2):4-6
124.朱春玉,吴元华,赵秀香.2007.嘧肽霉素对烟草花叶病毒在不同寄主上的防效研究.辽宁大学报,34(1):50-53
125.Abel R,IraidaA,VictorianoV,et al.ABA- and ethylene-mediated responses in osmotically stressed tomato are regulated by the TSS2 and TOS1 loci.Journal of Experimental Botany,57(12):3327-3335
126.Ada V,Michal H,Benjamin A.H,et al.2005.Trichoderma Mitogen-Activated Protein Kinase Signaling Is Involved in Induction of Plant Systemic Resistance.Applied and environmental microbiology,71(10):6241-6246
127.Agrawal G K,Rakwal R,Jwa N S,et al.2002.Molecular cloning and mRNA expression analysis of the first rice jasmonate biosynthetic pathway gene allene oxide synthase.Plant Physiology and Biochemistry,40:771 -782
128.Ajith A.,Srinivasa R.U.,Choong-M.R.,et al.2008.Salicylic Acid and Systemic Acquired Resistance Play a Role in Attenuating Crown Gall Disease Caused by 1[W][OA].Plant Physiol.146:703-715
129.Aleksandra W,Florina V,Caroline S,et al.2008.An Update on Abscisic Acid Signaling in Plants and More....Molecular Plant,1(2):198-217
130.Allan A C,Fluhr R.1997.Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells.The Plant Cell,9:1559-1572
131.Aloni R,Wolf A,Feigenbaum P,Avni A,Klee HJ.1998.The never ripe mutant provides evidence that tumor-induced ethylene controls the morphogenesis of Agrobacterium tumefaciens- induced crown galls in tomato stems.Plant Physiol,117:841-849
132.Alvarez M,Pennell R I,Vbijer PJ et al.1998.Reactive oxygen intermediates mediate a systemic signal net work in the establishment of plant immunity.Cell,92:773-784
133.Amano M,Toyoday K,Ichimosey et al.1997.Association between ion fluxes and defense responses in pea and cowpea tissues.Plant Cell Physiol,38(6):698 -706
134.AN Yu.SHEN Ying-bai.WU Li-juan.2006.A change of phenolic acids content in poplar leaves induced by methyl salicylate and methyl jasmonate.Journal of Forestry Research.17(2):107-110
135.Andrea A.L,Hiromasa Sa,Georg F,et al.2005.Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants.PNAS,102(30):10736-10741
136.Andrew J.H,Xinnian D.2006.Fitness Benefits of Systemic Acquired Resistance During Hyaloperonospora parasitica Infection in Arabidopsis thaliana Copyright,173:1621-1628
137.Androulla Gilliland .Davinder P.Singh .Jennifer M.Hayward.et al.2003.Genetic Modification of Alternative Respiration Has Differential Effects on Antimycin A-Induced versus Salicylic Acid-Induced Resistance to Tobacco mosaic virus.Plant Physiol.132:1518-1528
138.Ange'lique B.,Ce'cile C.,Adrien G.,et al.2008.Nitric Oxide in Plants:Production and Cross-talk with Ca~(2+) Signaling.Molecular Plant 1(2):218-228
139.B.KASSANIS,R.F.WHITE.1974.Inhibition of Acquired Resistance to Tobacco Mosaic Virus by Actinomycin D.Great Britain.25:323-324
140.Balachandran S,Osmond C B,Daley P F.1994.Diaynos is of the earliest stra-specific interactions between tobacco mosaic virus and chlorop lastes of tobacco leaves in vivo by means of chlorophyll fluorescence imaging [J].Plant Physiology,104(3):1059 -1065
141.Brad MB,Ronan C.O'M,Wuyi W,et al.2006.Ethylene Stimulates Nutations That Are Dependent on the ETR1 Receptor.Plant Physiology,142:1690-1700
142.Changhua Z,Lijun G,Zhenguo S,et al.2006.Interactions between jasmonates and ethylene in the regulation of root hair development in Arabidopsis.Journal of Experimental Botany,57(6):1299-1308
143.Chen KM,Gong HJ,Chen GC,Zhang CL.2002.ACC and MACC biosynthesis and ethylene production in water-stressed spring wheat.Acta Bot Sin,44(7):775-781
144.Chen N,Goodwin PH,Hsiang T.2003.The role of ethylene during the infection of Nicotiana tabacum by Colletotrichum destructivum.J Exp Bot,54(392):2449-2456
145.Creeiman R A.1992.Jasmonic acid/ methy jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression.Proc.Natl.Acad.Sci,89:4938—4941
146.Cristinzo G,Scala F.1994.Electrolyte leakage as a potential method for measuring of eggplant resistance and V.dahliae Virulence.Phytopathology,29(4):184 -190
147.Dean J F D,Gamble H R,Andersom J D.1989.The ethylene Biosynthesis- inducing xyganase:its induction in Trichoderma viride and certain plant pathogens [J].Phytopathology,79:1071-1078.
148.Dean R.A.,Kuc J.1986.Induced systemic protection in cucumber:time of production and movement of the signal.Phytopathology,76(10):966-970
149.Dean R.A.,Kuc J.1987.Rapid lignification in response to wounding and infection as a mechanism for induced systemic protection in cucumber.Physiological and Molecular Plant Phytopathology.31:69-81
150.Dempsey D,Shah J,Klessing D F.1999.Salicylic acid and disease resistance in plant [J].Critical Reviews in plant science,18(6):547-575
151.Dixon R A,Harrison M J,Lamb C J.1994.Early events in the activation of plant defense responses.Annu Rev Phytopathology.32:479-486
152.Doke N.1983.Generation of super oxide anion by potato tuber protoplasts upon the hypersensitive response to hyphal wall components of phytophthora infestans and specific inhibition of the reaction by suppressors of hypersensitive [J].Plant Physiology,23:359-367.
153.Dong H P,Peng J L,Bao Z L,et al.2004.Downstream divergence of the ethylene signaling pathway for harpin-stimulated Arabidopsis growth and insect defense.Plant Physiol,136:3628-3638
154.Dongtao R ,Kwang-YY ,Guo-JL,et al.2006.Activation of Ntf4,a Tobacco Mitogen-Activated Protein Kinase,during Plant Defense Response and Its Involvement in Hypersensitive Response-Like Cell Death.Plant Physiology,141:1482-1493
155.Droillard M J,Gudu J,Le Caer J P,et al.1997.Indentification of calreticulin like protein as one of the phosphoproteins,modulated in response to oligo galacturonides in tobacco ceddls.Planta,202(3):341-348
156.Durner J,Shah LKlessig D H.1997.Salicylic acid and disease resistance in plants.Trends Plant Sci,2:266-274
157.Durner J,Wendohenne D,Klessing D F.1997.Salicylic acid and disease resistance in plants.Trends Plant Sci,2:266-274
158.Durner J,Wendohenne D,Klessing D F.1998.Defense gene induction in tobacco by nitricoxide,cyclic ADP-ribose.Proc Natl Acad Sci USA,95:10328-10333
159.Ecker JR.1995.The ethylene signal transduction pathway in plants.Science,268(5):667-665
160.El-Sharkawy,W.S.K,A.E-K,et al.Isolation and characterization of four ethylene signal transduction elements in plums {Prunus salicina L.).ournal of Experimental Botany,58(13):3631-3643
161.Fawe A,Abou-Zaid M.1998.Silic on-mediated accumulation of flavonoid phytoalexins in cucumber.Phytopathology,88(5):396-401
162.Felix G,Regenass M,Boiler T.1993.Specific perception of subnanomolar concentrations of chitin fragments by tomato cells.Induction of extra cellular alkalinization changes in protein phosphorylation and establishment of a refractory state.Plant J,4(2):307-316
163.Fidanza M A,Dernoeden P H.1996.Interaction of potassium source application timing and fungicide on Rhizoctonia blight in ryegrass [J].Hort Sci,19(3):389-392.
164.Fuminor iT,Riichiro Y,Kazuya I,et al.2007.The Mitogen-Activated Protein Kinase Cascade MKK3-MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in Arabidopsis.ThePlantCell,19:805-818
165.Glenn E.B,Betty K.I.2007.Ethylene-sensitive and insensitive regulation of transcription factor expression during in vitro tomato sepal ripening.Journal of Experimental Botany,58(8):2043-2051
166.Godfrey P.M,MarcusA.S,Alan M.J,et al.2004.Mastoparan Rapidly Activates Plant MAP Kinase Signaling Independent of Heterotrimeric G Proteins.Plant Physiology,134:1332-1336
167.G(?)mez-Cadenas A,Tadeo F R,Primo-Millo E,et al.1998.Pathogen-induced systemic activation
168.Gundlach H,Muller M J,Kutchan T M,et al.1992.Jasmonic acid is a signal transducer in elicitor induced plant cellc ultures.Proc Natl Aced Sci USA,89:2389-2393
169.Hanhong B,Moon SK,Richard CS,et al.2006.Necrosis- and Ethylene-Inducing Peptide from Fusarium oxysporum Induces a Complex Cascade of Transcripts Associated with Signal Transduction and Cell Death in Arabidopsis.Plant Physiology,141:1056-1067
170.HELENA BIRECKA,JAMES L.CATALFAMO.PAUL URBAN.1975.Cell Wall and Protoplast Isoperoxidases in Tobacco Plants in Relation to Mechanical Injury and Infection with Tobacco Mosaic Virus.Pant Physiol.(1975) 55:611-619
171.Ingo H,Maria B-P,Katarina H,et al.2005.Virus-Induced Gene Silencing-Based Functional Characterization of Genes Associated with Powdery Mildew Resistance in Barley.Plant Physiology,138:2155-2164
172.Javier A,Paz M,Manuel C,et al.2008.Ethylene-induced differential gene expression during abscission of citrus leaves.Journal of Experimental Botany,59(10):2717-2733
173.Justin L,Daniel FK,Thorsten N.2001.A Harpin Binding Site in Tobacco Plasma Membranes Mediates Activation of the Pathogenesis-Related Gene HIN1 Independent of Extracellular Calcium but Dependent on Mitogen-Activated Protein Kinase Activity.The Plant Cell,13:1079-1093
174.Kaoru S,Akira Y,Hideaki S.1999.Slow and Prolonged Activation of the p47 Protein Kinase during Hypersensitive Cell Death in a Culture of Tobacco Cells.Plant Physiology,19:1465-1472
175.Kerry F.P,Gregory B.M.2004.Identification of MAPKs and Their Possible MAPK Kinase Activators Involved in the Pto-mediated Defense Response of Tomato.THE JOURNAL OF BIOLOGICAL CHEMISTRY,279(47):49229-49235
176.Klarzynski O,Descamps V,Plesse B,et al.2003.Sulfated fucan oligosaccharides elicit defense responses in tobacco and local and systemic resistance against tobacco mosaic virus.Molecular plant-Microbe Interaction,16(2):115-122
177.Klarzynski O,Plesse B,Joubert J M,et al.2000.Linear B-1,3 Glucans are elicitors of defense responses in tobacco.Plant Physiol,124(3):1027-1038
178.Klessing D F,Durner J,Noad R,et al.2000.Nitric oxide and salicylic acid signaling in plant defense.Proc Natl Acad Sci USA.,97:8849-8855
179.Klessing D F,Malamy J.1994.The salicylic acid signal in plants.Plant Mol Bind,26:1439-1458.
180.Kuchitsu K,Kikuyama M,Shibuya N.1993.N-acetyl-chito oligo saccharides biotic elicitor for phytoalexin production,induce transient membrane depolarization in suspension-cultured rice cell.Protoplasma,174(1-2):79-81
181.Kurosaki F,Tsurusawa Y,Nishi A.1987.Break down of phosphatidylinositol during the elicitation of phytoalex in production in cultured carrot cells.Plant Physiol,85:601
182.Kurstak E.1981.Handbook of plant virus infections and comparative diagnosis [M],Elsevier/North-Holland:Biomedical Press,258-277.
183.Kwang-Yeol Y,Yidong L,Shuqun Z.2001.Activation of a mitogen-activated protein kinase pathway is involved in disease resistance in tobacco.PNAS,98(2):741-746
184.Linda M.R,Jessica S.W,Matthew J.C,et al.2007.Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response,including ERF1 induction.Journal of Experimental Botany,58(10):2627-2639
185.Livio T,Alice T,Giorgio C.2007.The involvement of auxin in the ripening of climacteric fruits comes of age:the hormone plays a role of its own and has an intense interplay with ethylene in ripening peaches.Journal of Experimental Botany,58(12):3299-3308
186.Malamy J,Carr J P,Klessig D F,et al.1990.Salicylic acid:a likely endogenous signal in the resistance response of tobacco to viral infection.Science,250:1002-1004
187.Marcus A.S,Ankit W,Shawn D.M,et al.2005.Overexpression of SIPK in tobacco enhances ozone-induced ethylene formation and blocks ozone-induced SA accumulation.Journal of Experimental Botany,56(418):2195-2201
188.Masanori T,John LF,Elizabeth A.H.P-S.2008.Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in ArabidopsisPlant.Physiology,146:1219-1230
189.Matsuda F,Miyagawa H,Ueno T.2001.Involvement of reactive oxygen species in the induction of(S)-N-p-coumaroyloctopamine accumulation by beta-l,3-glucooligosaccharide elicitors in potato tuber tissues.Z Natu rforsch,56(3-4):228-234
190.Mayer M G,et al.1988.An elicitor from Phytophthora megasperma f.sp.glycinea influences the membrane potential of soybean cotyledonar cell.Physiological and Molecular Plant Pathology,33:397-407
191.Mitter,Neena,Kazan,et al.1998.Systemic induction of an Arabidopsis plant defensin gene promoter by tobacco mosaic virus and jasmonic acid in transgenic tobacco.Plant Science,136(2):169-180
192.NING Jing.YUAN Bin.XIE Ka-Bin.et al.2006.Isolation and Identification of SA and JA Inducible Protein Kinase Gene OsSJMKl in Rice.Acta Genetica Sinica.33(7):625-633
193.Ornella C,Nathalie G,Catherine B,et al.2001.A Novel Tobacco Mitogen-activated Protein(MAP) Kinase Kinase,NtMEKl,Activates the Cell Cycle-regulated p43~(Ntf6) MAP Kinase.THE JOURNAL OF BIOLOGICAL CHEMISTRY,276(21):18139-18145
194.Orozco-Cardenas M L,Ryan C A.2002.Nitric oxide negatively modulates wound signaling in tomato plants.Plant Physiol,130(1):487-493
195.Peng M,Kuc J.1992.Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks [J].Phytopathology,82:718-722.
196.Penninckx IAMA,Thomma BPHJ,Buchala A,et al.1998.Concomitant activation of jasmonate
197.Philippe R,Auzanne G,Kalanethee P,et al.1995.Oligogalacturonide defense signal in plants:Large fragments interact with the plasma membrane in vitro.Proc Natl Acad Sci USA,92:4145-4149
198.Revmond P,Farmer E E.1998.Jasmonate and salicylate as global signals for defense gene expression.Current Opinion in Plant Biology,1(5):404-411
199.Reymond P,Grunberger S,Paul K,Muller M.1995.Oligosaccharide defence signals in plants:largefrgmants interact with the plasmam embrane in vitro.Froc Nail Acad Sci USA,92:4145-4149
200.Robert J.S,Ellen N.F,Edwige J.F.S,et al.2007.A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway.Plant Physiology,144:1899-1912
201.Schaller F.2001.Enzymes of the biosynthesis of octadecanoide-derived signaling moleculars Journal of Experimental Botany,52:11-23
202.Shuqun Z,Daniel F.Klessig.1997.Salicylic Acid Activates a 48-kD MAP Kinase in Tobacco.The Plant Cell,9:809-824
203.Shuqun Z,Daniel F.K.1998.Resistance gene N-mediated de novo synthesis and activation of a obacco mitogen-activated protein kinase by tobacco mosaic irusinfection.Proc.Natl.Acad.Sci.USA,95:433-7438
204.Shuqun Z,He D,Daniel F.Klessig.1998.Activation of the Tobacco SIP Kinase by Both a Cell Wall-Derived Carbohydrate Elicitor and Purified Proteinaceous Elicitins from Phytophthora spp.The Plant Cell,10:425-449
205.Shuqun Z,Yidong L.2001.Activation of Salicylic Acid-Induced Protein Kinase,a Mitogen-Activated Protein Kinase,Induces Multiple Defense Responses in Tobacco.The Plant Cell,13:1877-1889
206.ShutaAsai.K.,Hirofumi Y.2008.MAPK Signaling Regulates Nitric Oxide.and NADPH Oxidase- Dependent Oxidative Bursts in WOA Nicotiana benthamiana.American Society of Plant Biologists.20:1390-1406
207.Simone F,Roberta G,Carine D,et al.2007.Resistance to Botrytis cinerea Induced in Arabidopsis by Elicitors Is Independent of Salicylic Acid,Ethylene,or Jasmonate signaling but requires PHYTOALEXIN DEFICIENT3.Plant Physiol,144:367-379
208.Simpson S D,Ashford D A,Jarvey D J,et al.1998.Short chain oligogalacturobides induce ethylene production and expression of the gene encoding aminocvclopropane 1-carboxvlic acid oxides in tomato plants.Glvcobiol,8(6):579-583
209.Sjoerd VE,Bas WMV,Ronald VD,et al.2008.MYB72 Is Required in Early Signaling Steps of Rhizobacteria-Induced Systemic Resistance in Arabidopsis.Plant Physiology,146:1293-1304
210.Solomos T,Laties G G.1976.Ef fect of cyanide and ethylene on the respirarion of cyanide sensitive and cyanide-resistant plant tissues.Plant Physiol,58:47-50
211.Song F,Goodman R M.2001.Activity of nitric oxide is dependent on,but is partially required for function of salicylic acid in the signaling pathway in tobacco systemic acquired resistance.Mol Plant Microbe Interact,14(12):1458-1462
212.SONG Zhen-xia.GAO Ming.WANGLi-ao,et al.2008.Effects of Boron Fertilizer Application Rates on the Yieldand Quality of Flue2Cured Tobacco.Journal of Southwest University.30(6):64-72
213.Strasser H,Hottman C,Grisebach H,et al.1986.Are polyphosphoinositides involved in signal transduction of elicitor induced phytoalexin synthesis in cultured plant cell ? Z Naturforsch,41:717
214.Tang D,Christiansen KM,Innes RW.2005.Regulation of plant disease resistance,stress responses,cell death,and ethylene signaling in Arabidopsis by the EDR1 protein kinase.Plant Physiol,138:1018-1026
215.Taro O,Ling P,Maki K-Y,et al.2005.Functional Analysis of Arabidopsis Ethylene-Responsive Element Binding Protein Conferring Resistance to Bax and Abiotic Stress-Induced Plant Cell Death.Plant Physiology,138:1436-1445
216.Tatiana EM,Jurgen Z.2006.The Arabidopsis Flavin-Dependent Monooxygenase FMO1 Is an Essential Component of Biologically Induced Systemic Acquired Resistance.Plant Physiology,141:1666-1675
217.teven R.S,Li H,Amanda S.B,et al.2005.Development of a Virus-Induced Gene-Silencing System for Hexaploid Wheat and Its Use in Functional Analysis of the Lr21-Mediated Leaf Rust Resistance Pathway.Plant Physiology,138:2165-2173
218.Thomas P,Ame'rin V,Brad M.B,et al.2006.The Exoribonuclease XRN4 Is a Component of the Ethylene Response Pathway in Arabidopsis.The Plant Cell,18:3047-3057
219.Tina R,Pedro P,Shuqun Z,et al.1999.Rapid Avr9- and Cf-9-Dependent Activation of MAP Kinases in Tobacco Cell Cultures and Leaves:Convergence of Resistance Gene,Elicitor,Wound,and Salicylate Responses.The Plant Cell,11:273-287
220.Tsuyoshi Mi,Kenji,Iriet,et al.1996.A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch,cold,and water stress in Arabidopsis thaliana.Proc.Natl.Acad.Sci.USA,93:765-769
221.Vranova E,Inze D,Breusegem FV.2002.Signal transdution during oxidative stress.J Exp Bot,53:1227-1236.
222.Walter E,Riedell,Shannon L.2007.Crop mineral nutrient and yield responses to aphids or barley yellow dwarf virus in spring wheat and oat [J].Crop Science,47:1553-1560.
223.Warington K.1923.The effect of boric acid and borax on the broad bean and certain other plants .Ann Bot,37:629-672.
224.Yang et al.1997.Signal perception and transduction in plant defense response.Genes Dev,1621-1639.
225.Yi-Feng C,Naomi E,G.Eric Schaller.2005.Ethylene Signal Transduction.Annals of Botany,95:901-915