高糖日粮对小鼠消化系统自由基和抗氧化能力的影响
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摘要
高糖膳食可导致机体糖代谢紊乱和氧化应激,引发肥胖、糖尿病和心脑血管疾病等多种代谢综合症,而氧自由基(reactive oxygen special, ROS)是介导糖代谢紊乱到代谢综合症的病理因子。本论文研究了灌胃高糖对小鼠血糖浓度和血液中ROS的影响、高糖日粮对小鼠消化系统ROS和抗氧化能力的影响,并研究了硫辛酸(lipoic acid, LA)和丹酚酸B(salvianolic acid B, Sal B)对高糖日粮小鼠的消化系统和抗氧化能力的影响,为高糖膳食引发的各种慢性代谢疾病的发病机理和调控提供理论依据。
选取昆明种雄性小鼠80只,按体重随机分成8组,饥饿过夜后分别灌胃生理盐水、葡萄糖、果糖、半乳糖、白糖、冰糖、红糖和葡萄糖+Sal B,然后选择在0 h、0.5 h、1.0 h、1.5 h、2.0 h时间点,剪尾取血,测定血液ROS和血糖浓度,其中糖和Sal B的灌胃量分别为平均一次采食量的20%和0.002%。另取昆明种雌性小鼠60只,按体重随机分成6组,即正常对照组(正常日粮)、高糖日粮组(高糖日粮)、LAⅰ组(高糖日粮+0.05%LA)、LAⅱ组(高糖日粮+0.1%LA)、Sal Bⅰ组(高糖日粮+0.002%Sal B)和Sal Bⅱ组(高糖日粮+0.004%Sal B),4周后测定各组小鼠血液和消化系统各组织ROS和抗氧化指标。实验结果显示:(1)灌胃单糖后小鼠的血液ROS释放量为:葡萄糖>果糖>半乳糖;灌胃双糖后小鼠的血液ROS释放量:白糖>冰糖>红糖;(2)高糖日粮导致小鼠血液和消化系统ROS释放量增加,改变了各组织ROS释放量占整个消化系统总量的比例,肝脏和胰腺的比例显著提高,造成了严重的氧化应激;(3)LA能够清除高糖日粮小鼠血液和消化系统中产生的过多的ROS,能够提高高糖日粮小鼠机体的抗氧化能力,并且0.1%的剂量好于0.05%;(4)0.002%的Sal B能够降低灌胃高糖小鼠的血糖浓度,减少ROS释放量,能够清除高糖日粮小鼠血液和消化系统中产生的过多的ROS,能显著提高高糖日粮小鼠机体的抗氧化能力,但0.004%的Sal B不能清除高糖日粮小鼠血液和消化吸收系统中产生的过多的ROS,不能提高高糖日粮小鼠机体的抗氧化能力。
High sugar diet can cause glucose metablolism disorder and oxidative stress, leading to metabolic syndrome such as obesity, diabetes mellitus and cardiovascular diseases. While reactive oxygen special (ROS) is a determinant factor from glucose metablolism disorder to metabolic syndrome. This research is focused on following aspects: the effects on blood glucose and ROS level of intragastric(ig) high sugar mice; the effects of high sugar diet on digestive system ROS level and antioxidation ability of mice; the effects of lipoic acid(LA) and salvianolic acid B (Sal B) on digestive system ROS level and antioxidation ability of mice fed with high sugar diet. This research contributes to the pathology mechanism and regulation of chronic metabolic syndrome.
80 Kunming mice were randomly divided into 8 groups. After a fast of 12 h, mice were administered respectively with saline, 20% glucose, 20% fructose, 20% galactose, 20% white sugar, 20% crystal sugar, 20% brown sugar, and 20% glucose + 0.002% Sal B. Blood glucose and the ROS level were determined at 0 h, 0.5 h, 1.0 h, 1.5 h, 2.0 h after administration. Another 6 groups of ten Kunming mice were fed with normal diet, high sugar diet (20% sucrose), high sugar diet with LA (20% sucrose + 0.05% LA), high sugar diet with LA (20% sucrose + 0.1% LA), high sugar diet with Sal B (20% sucrose + 0.002% Sal B) and high sugar diet with Sal B (20% sucrose + 0.004% Sal B), respectively. After 4 weeks, the ROS level and antioxidation ability of digestive system and blood were determined. Results were as follows: (1) ROS levels of ig monosaccharide mice: glucose > fructose > galactose; ROS levels of ig disaccharide mice: white sugar > brown sugar > crystal sugar; (2) High sugar diet led to increase of ROS level of blood and digestive system; the digestive system tissues’contributions to ROS level were changed: the contributions of liver and pancreas were significantly increased in which oxidative stress were most serious; (3) LA can scavenge ROS in blood and digestive system, increase the antioxidation ability of high sugar dietary mice, and the effect is better at a high concentration (0.1%); (4) 0.002% Sal B can lower the blood glucose, reduce ROS level in blood and digestive system, and significantly improve the antioxidation ability of mice fed with high sugar diet, while 0.004% Sal B can not.
选取昆明种雄性小鼠80只,按体重随机分成8组,饥饿过夜后分别灌胃生理盐水、葡萄糖、果糖、半乳糖、白糖、冰糖、红糖和葡萄糖+Sal B,然后选择在0 h、0.5 h、1.0 h、1.5 h、2.0 h时间点,剪尾取血,测定血液ROS和血糖浓度,其中糖和Sal B的灌胃量分别为平均一次采食量的20%和0.002%。另取昆明种雌性小鼠60只,按体重随机分成6组,即正常对照组(正常日粮)、高糖日粮组(高糖日粮)、LAⅰ组(高糖日粮+0.05%LA)、LAⅱ组(高糖日粮+0.1%LA)、Sal Bⅰ组(高糖日粮+0.002%Sal B)和Sal Bⅱ组(高糖日粮+0.004%Sal B),4周后测定各组小鼠血液和消化系统各组织ROS和抗氧化指标。实验结果显示:(1)灌胃单糖后小鼠的血液ROS释放量为:葡萄糖>果糖>半乳糖;灌胃双糖后小鼠的血液ROS释放量:白糖>冰糖>红糖;(2)高糖日粮导致小鼠血液和消化系统ROS释放量增加,改变了各组织ROS释放量占整个消化系统总量的比例,肝脏和胰腺的比例显著提高,造成了严重的氧化应激;(3)LA能够清除高糖日粮小鼠血液和消化系统中产生的过多的ROS,能够提高高糖日粮小鼠机体的抗氧化能力,并且0.1%的剂量好于0.05%;(4)0.002%的Sal B能够降低灌胃高糖小鼠的血糖浓度,减少ROS释放量,能够清除高糖日粮小鼠血液和消化系统中产生的过多的ROS,能显著提高高糖日粮小鼠机体的抗氧化能力,但0.004%的Sal B不能清除高糖日粮小鼠血液和消化吸收系统中产生的过多的ROS,不能提高高糖日粮小鼠机体的抗氧化能力。
High sugar diet can cause glucose metablolism disorder and oxidative stress, leading to metabolic syndrome such as obesity, diabetes mellitus and cardiovascular diseases. While reactive oxygen special (ROS) is a determinant factor from glucose metablolism disorder to metabolic syndrome. This research is focused on following aspects: the effects on blood glucose and ROS level of intragastric(ig) high sugar mice; the effects of high sugar diet on digestive system ROS level and antioxidation ability of mice; the effects of lipoic acid(LA) and salvianolic acid B (Sal B) on digestive system ROS level and antioxidation ability of mice fed with high sugar diet. This research contributes to the pathology mechanism and regulation of chronic metabolic syndrome.
80 Kunming mice were randomly divided into 8 groups. After a fast of 12 h, mice were administered respectively with saline, 20% glucose, 20% fructose, 20% galactose, 20% white sugar, 20% crystal sugar, 20% brown sugar, and 20% glucose + 0.002% Sal B. Blood glucose and the ROS level were determined at 0 h, 0.5 h, 1.0 h, 1.5 h, 2.0 h after administration. Another 6 groups of ten Kunming mice were fed with normal diet, high sugar diet (20% sucrose), high sugar diet with LA (20% sucrose + 0.05% LA), high sugar diet with LA (20% sucrose + 0.1% LA), high sugar diet with Sal B (20% sucrose + 0.002% Sal B) and high sugar diet with Sal B (20% sucrose + 0.004% Sal B), respectively. After 4 weeks, the ROS level and antioxidation ability of digestive system and blood were determined. Results were as follows: (1) ROS levels of ig monosaccharide mice: glucose > fructose > galactose; ROS levels of ig disaccharide mice: white sugar > brown sugar > crystal sugar; (2) High sugar diet led to increase of ROS level of blood and digestive system; the digestive system tissues’contributions to ROS level were changed: the contributions of liver and pancreas were significantly increased in which oxidative stress were most serious; (3) LA can scavenge ROS in blood and digestive system, increase the antioxidation ability of high sugar dietary mice, and the effect is better at a high concentration (0.1%); (4) 0.002% Sal B can lower the blood glucose, reduce ROS level in blood and digestive system, and significantly improve the antioxidation ability of mice fed with high sugar diet, while 0.004% Sal B can not.
引文
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