两种水稻病毒胁迫下水稻的生物信息学研究
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摘要
水稻矮缩病毒(Rice dwarf virus,RDV)和水稻条纹病毒(Rice stripe virus,RSV)是两种对水稻生长有着重大影响的病毒,对我国水稻生产造成巨大威胁。RDV是呼肠孤病毒科(Reoviridae)、植物呼肠孤病毒属(Phytoreovirus)的成员,是水稻普通矮缩病的病源,广泛分布于中国、日本、朝鲜、菲律宾、尼泊尔等东南亚水稻种植区。RSV是纤细病毒属(Tenuivirus)的代表种,其分布更为广泛,因此危害也更严重。病毒与寄主互作的分子机制研究表明,病毒侵染后寄主会发生一系列防御反应。从转录组水平上揭示在病毒胁迫下的水稻抗病相关基因的表达模型,对于研究病毒与寄主互作的分子机制具有重要的理论和应用意义。本文在全面分析水稻分别受RDV和RSV胁迫下的基因表达谱数据的基础上,从对抗病与感病品种的比较、RDV与RSV胁迫下基因表达谱的变化这两个角度,利用计算机编程方便快捷的处理数据,进行大规模的生物信息学分析,得出水稻在病毒胁迫下的几条显著差异且具有代表性的代谢途径(pathway)。本文还着重通过对植物激素——赤霉素(gibberellin)代谢及其调控的生物信息学分析,深入探讨水稻受病毒胁迫的机理。
Rice dwarf virus (RDV) and Rice stripe virus(RSV) both have made a great impact on rice growth.Whether RDV outbreaks or RSV outbreaks resulted in a considerable decrease in production.Rice dwarf virus is the member of the first family of Phytoreovirus, Reoviridae. It is the pathogen for ordinary dwarf diseases of rice and widely distributed in rice growth regions in Southeast Asian, such as Japan, Korea, Philippines and Nepal. RDV disease commonly occurred and even ran rampant in rice production areas in the south of China . It has caused great decreases to rice yields in China. And Rice stripe virus is the typical species of the first family of Tenuivirus.
The researches on the molecular mechanisms of virus-host interactions indicated that a number of defense reactions were induced after viral infection. It is of profound theoretical and practical significance to reveal the expression model of the related resistance genes under the stress of virus at the transcriptional level. To adopt Both-threaded method research this thesis.
Two pilot schemes were carried out respectively. Based on transcriptom profilings obtained previously in our lab, expression differences between the resistent and susceptable rice varieties in response to RSV infection and between rice responses to RDV and RSV infection were compaired using specific programs in this study. Several pathways were shown to be differentialy altered in different rice/variaty-virus combinations. Among them were those involved in the metebolitics of gibberellins. Implications of the findings and the possible involvement of gibberellins in rice-virus interactions were discussed.
Rice dwarf virus (RDV) and Rice stripe virus(RSV) both have made a great impact on rice growth.Whether RDV outbreaks or RSV outbreaks resulted in a considerable decrease in production.Rice dwarf virus is the member of the first family of Phytoreovirus, Reoviridae. It is the pathogen for ordinary dwarf diseases of rice and widely distributed in rice growth regions in Southeast Asian, such as Japan, Korea, Philippines and Nepal. RDV disease commonly occurred and even ran rampant in rice production areas in the south of China . It has caused great decreases to rice yields in China. And Rice stripe virus is the typical species of the first family of Tenuivirus.
The researches on the molecular mechanisms of virus-host interactions indicated that a number of defense reactions were induced after viral infection. It is of profound theoretical and practical significance to reveal the expression model of the related resistance genes under the stress of virus at the transcriptional level. To adopt Both-threaded method research this thesis.
Two pilot schemes were carried out respectively. Based on transcriptom profilings obtained previously in our lab, expression differences between the resistent and susceptable rice varieties in response to RSV infection and between rice responses to RDV and RSV infection were compaired using specific programs in this study. Several pathways were shown to be differentialy altered in different rice/variaty-virus combinations. Among them were those involved in the metebolitics of gibberellins. Implications of the findings and the possible involvement of gibberellins in rice-virus interactions were discussed.
引文
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