鄱阳湖水体细菌物种多样性研究
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摘要
本研究采用细菌16SrDNA序列分析的分子生物学技术对鄱阳湖南北主湖区水体细菌多样性进行了系统研究。其结果如下:
①.北湖平水期(2006年10月样)水域的细菌分属于八大细菌类群:变形菌门的α-,β-,γ-,Epsilon和未知分类地位的变形细菌纲,放线菌门,拟杆菌门,酸杆菌门,浮霉菌门。
②.北湖丰水期(2007年5月样)水域的细菌分属于以下七大细菌类群中:变形菌门的α-,β-,γ-变形细菌纲,放线菌门,拟杆菌门,OP10,浮霉菌门,以及未知分类地位细菌。
③.南湖平水期水域(2006年10月样)的细菌分属于以下九大细菌类群中:变形菌门的α-,β-,γ-变形细菌门,放线菌门,拟杆菌门,OP10,蓝细菌,疣微菌门,硝化螺旋菌门以及未知分类地位细菌。
④.南湖丰水期(2007年5月样)水域的细菌分属于以下八大细菌类群中:变形菌门的α-,β-,γ-变形细菌纲,放线菌门,拟杆菌门,蓝细菌,疣微菌门,浮霉菌以及未知分类地位细菌。
鄱阳湖水体细菌主要由不可培养菌组成,细菌群落明显受季节影响,南湖水体细菌的丰富度和多样性高于北湖。北湖平水期和丰水期水体优势菌分别为β-变形菌和γ-变形菌+拟杆菌;南湖平水期和丰水期水体优势菌分别为β-变形菌纲+放线菌和β-变形菌。鄱阳湖水环境细菌群落受到了人类活动的影响。
In this study,bacterial community compositions in the northern and southern water area of Lake Poyang were analyzed firstly by 16S rDNA sequence analysis. The main conclusions were presented as follow:
①.Bacterial community composition from North water area of Lake Poyang in Oct.2006 belongs to the following eight groups of the domain bacteria: Alpha-proteobacteria、Beta-proteobacteria、Gamma-proteobacteria、Epsilon-proteobacteria、Actinobacteria、Bacteroidetes、Acidobacteria、Planctomycates and unclassified proteobacteria.
②.Bacterial community composition from North water area of Lake Poyang in May.2007 belongs to the following seven groups of the domain Bacteria: Alpha-proteobacteria、Beta-proteobacteria、Gamma-proteobacteria、Actinobacteria、Bacteroidetes、OP10、Planctomycetes and the unclassified bacteria.
③.Bacterial community composition from South Lake Poyang in Oct.2006 was affiliated with nine bacterial divisions,e.g.,Alpha-proteobacteria, Beta-proteobacteria,Gamma-proteobacteria,Actinobacteria,Bacteroidetes,OP10, Cyanobacteria,Verrucomicrobia,Nitrospira,unclassified bacterium.
④.Bacterial community composition from South Lake Poyang in May.2007 was affiliated with eight bacterial divisions,e.g.,Alpha-proteobacteria, Beta-proteobacteria,Gamma-proteobacteria,Actinobacteria,Bacteroidetes, Cyanobacteria,Verrucomicrobia,Planctomycetes,unclassified bacterium.
Results indicated that bacteria had rich diversity and apparently temporal and spatial heterogeneity from water of Lake Poyang.Bacterial compositions were affected by season,and the bacterial diversity and richeness from southern water was greater than from northem water.Beta-proteobacteria was the most significant lineages in the northern(2006) and southern(2007) Lake Poyang.However, Gamma-proteobacteria and Bacteroidetes were the most significant lineages in the northern(2007) Lake Poyang,while Beta-proteobacteria and Actinobacteria were the significant lineages in the southern(2006) Poyang.
①.北湖平水期(2006年10月样)水域的细菌分属于八大细菌类群:变形菌门的α-,β-,γ-,Epsilon和未知分类地位的变形细菌纲,放线菌门,拟杆菌门,酸杆菌门,浮霉菌门。
②.北湖丰水期(2007年5月样)水域的细菌分属于以下七大细菌类群中:变形菌门的α-,β-,γ-变形细菌纲,放线菌门,拟杆菌门,OP10,浮霉菌门,以及未知分类地位细菌。
③.南湖平水期水域(2006年10月样)的细菌分属于以下九大细菌类群中:变形菌门的α-,β-,γ-变形细菌门,放线菌门,拟杆菌门,OP10,蓝细菌,疣微菌门,硝化螺旋菌门以及未知分类地位细菌。
④.南湖丰水期(2007年5月样)水域的细菌分属于以下八大细菌类群中:变形菌门的α-,β-,γ-变形细菌纲,放线菌门,拟杆菌门,蓝细菌,疣微菌门,浮霉菌以及未知分类地位细菌。
鄱阳湖水体细菌主要由不可培养菌组成,细菌群落明显受季节影响,南湖水体细菌的丰富度和多样性高于北湖。北湖平水期和丰水期水体优势菌分别为β-变形菌和γ-变形菌+拟杆菌;南湖平水期和丰水期水体优势菌分别为β-变形菌纲+放线菌和β-变形菌。鄱阳湖水环境细菌群落受到了人类活动的影响。
In this study,bacterial community compositions in the northern and southern water area of Lake Poyang were analyzed firstly by 16S rDNA sequence analysis. The main conclusions were presented as follow:
①.Bacterial community composition from North water area of Lake Poyang in Oct.2006 belongs to the following eight groups of the domain bacteria: Alpha-proteobacteria、Beta-proteobacteria、Gamma-proteobacteria、Epsilon-proteobacteria、Actinobacteria、Bacteroidetes、Acidobacteria、Planctomycates and unclassified proteobacteria.
②.Bacterial community composition from North water area of Lake Poyang in May.2007 belongs to the following seven groups of the domain Bacteria: Alpha-proteobacteria、Beta-proteobacteria、Gamma-proteobacteria、Actinobacteria、Bacteroidetes、OP10、Planctomycetes and the unclassified bacteria.
③.Bacterial community composition from South Lake Poyang in Oct.2006 was affiliated with nine bacterial divisions,e.g.,Alpha-proteobacteria, Beta-proteobacteria,Gamma-proteobacteria,Actinobacteria,Bacteroidetes,OP10, Cyanobacteria,Verrucomicrobia,Nitrospira,unclassified bacterium.
④.Bacterial community composition from South Lake Poyang in May.2007 was affiliated with eight bacterial divisions,e.g.,Alpha-proteobacteria, Beta-proteobacteria,Gamma-proteobacteria,Actinobacteria,Bacteroidetes, Cyanobacteria,Verrucomicrobia,Planctomycetes,unclassified bacterium.
Results indicated that bacteria had rich diversity and apparently temporal and spatial heterogeneity from water of Lake Poyang.Bacterial compositions were affected by season,and the bacterial diversity and richeness from southern water was greater than from northem water.Beta-proteobacteria was the most significant lineages in the northern(2006) and southern(2007) Lake Poyang.However, Gamma-proteobacteria and Bacteroidetes were the most significant lineages in the northern(2007) Lake Poyang,while Beta-proteobacteria and Actinobacteria were the significant lineages in the southern(2006) Poyang.
引文
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