水稻生育后期耐热性机理及其鉴定指标的筛选
|
摘要
在高温、大田对照两种条件下用不同品种分别对水稻开花期、灌浆期和全生育期的生长发育和生理生化等性状特征进行了系统研究和分析,初步建立了水稻开花期和灌浆期的耐热性鉴定指标,深入研究了高温胁迫对水稻生理、生化、产量等指标的影响,充分论证了高温胁迫下水稻生理生化特性与品种耐热性的关系,揭示了决定水稻耐热性的关健生理生化机制。主要结果如下:
1.水稻开花期高温胁迫降低了叶片光合速率、叶绿素含量,增加了可溶性糖、游离脯氨酸、MDA含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性。通过相关分析表明,开花期高温胁迫与对照条件下的叶绿素、游离脯氨酸、MDA含量和SOD活性的变化幅度与品种的热敏感指数呈显著或极显著相关性,因此,高温下的叶绿素、游离脯氨酸、MDA含量和SOD活性的变化幅度可作为水稻开花期耐热性生理鉴定指标。对此4项指标变化幅度与热敏感指数进行逐步回归分析,所建立的回归方程可预测水稻开花期耐热性。
2.灌浆期高温胁迫严重影响水稻的光合能力,酶保护机制和渗透调节能力。叶片光合速率,叶绿素、可溶性糖、蛋白质、MDA含量和SOD活性都是与水稻灌浆期耐热性密切相关的生理生化指标。但不同品种灌浆期耐热性存在相当大的差异。
3.以水稻叶片光合速率,叶绿素、可溶性糖、蛋白质、MDA含量和SOD活性等与灌浆期耐热性密切相关性状的耐热指数为指标,应用主成分分析法将其综合成2个新的彼此独立的综合指标,再利用隶属函数值法求出每一品种耐热性的综合评价值,可较为全面、准确地评判各水稻品种的耐热性强弱。
4.全生育期高温胁迫加速了水稻植株衰老和保护性功能的丧失,导致生育后期水稻叶片酶保护性机制失活,叶片渗透调节能力降低,干物质生产减少,茎鞘物质转运受阻,茎鞘物质输出率和转换率都显著下降,导致产量显著降低。在整个大环境升温情况下,应该注重生育中后期的田间管理。
The experiment was carried out in growth chamber under both normal and high temperature to investigate the effects of heat stress on morphological, growth and development indexes and physiological and biochemical characteristics during flowering, grain filling stage and the whole growth stage. Identification index of heat resistance during flowering and grain filling stage had been established preliminary. The effects of heat stress on morphological, physiological, biochemical and grain yield had been carried out deep research. This paper adequately expounded and proved the relationship between physiological and biochemical characteristics and heat resistance in rice and discussed the key physiological and biochemical mechanism of heat resistance during grain flowering stage. The results followed:
1. High temperature stress on flowering stage decreased leaves' photosynthesis rate, contents of chlorophyll, increased contents of soluble sugar content, free proline content, malondialdehyde (MDA) content, as well as the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in leaves at flowering stage. Correlation analysis showed that heat susceptibility index significantly or very significantly correlated with relative changed percentage of contents of chlorophyll, free proline, MDA and SOD activity under both high temperature and normal conditions. Therefore, they could be selected as physiological identification indexes for heat tolerance at flowering stage in rice. Using stepwise regression equation to evaluate the heat tolerance of rice cultivars was considered scientifically and reliably.
2. The ability of photosynthesis, osmotic adjustment and antioxidative enzyme activities were effected significantly in the high temperature condition at grain filling stage. The content of chlorophyll, soluble sugar, protein ,MDA, photosynthesis rate and the activity of SOD were correlated with heat resistance on grain filling stage. And heat tolerance on grain filling stage were different because of different cultivars.
3. The content of chlorophyll, soluble sugar, protein, MDA, photosynthesis rate and the activity of SOD are correlative with heat tolerance at grain filling stage, which were synthesized to two new independent comprehensive indices by principal component analysis, Then the comprehensive evaluation of every rice varieties' heat tolerance was obtained by subordinate function value method (SFVM), Consequently, each cultivar' heat tolerance could be evaluated completely and correctly.
4. The high temperature stress in the whole growth stage accelerated rice caducity and loss of protected functions, also lost the activities of antioxidative enzymes in leaves, decreased the osmotic adjustment of leaves, reduced the dry matters production, blocked the translocation and transformation of dry matters in stems and sheaths, decreased yields significantly. In the condition of higher environmental temperature, it should be important to strengthen the farming management in the late growth stage.
1.水稻开花期高温胁迫降低了叶片光合速率、叶绿素含量,增加了可溶性糖、游离脯氨酸、MDA含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性。通过相关分析表明,开花期高温胁迫与对照条件下的叶绿素、游离脯氨酸、MDA含量和SOD活性的变化幅度与品种的热敏感指数呈显著或极显著相关性,因此,高温下的叶绿素、游离脯氨酸、MDA含量和SOD活性的变化幅度可作为水稻开花期耐热性生理鉴定指标。对此4项指标变化幅度与热敏感指数进行逐步回归分析,所建立的回归方程可预测水稻开花期耐热性。
2.灌浆期高温胁迫严重影响水稻的光合能力,酶保护机制和渗透调节能力。叶片光合速率,叶绿素、可溶性糖、蛋白质、MDA含量和SOD活性都是与水稻灌浆期耐热性密切相关的生理生化指标。但不同品种灌浆期耐热性存在相当大的差异。
3.以水稻叶片光合速率,叶绿素、可溶性糖、蛋白质、MDA含量和SOD活性等与灌浆期耐热性密切相关性状的耐热指数为指标,应用主成分分析法将其综合成2个新的彼此独立的综合指标,再利用隶属函数值法求出每一品种耐热性的综合评价值,可较为全面、准确地评判各水稻品种的耐热性强弱。
4.全生育期高温胁迫加速了水稻植株衰老和保护性功能的丧失,导致生育后期水稻叶片酶保护性机制失活,叶片渗透调节能力降低,干物质生产减少,茎鞘物质转运受阻,茎鞘物质输出率和转换率都显著下降,导致产量显著降低。在整个大环境升温情况下,应该注重生育中后期的田间管理。
The experiment was carried out in growth chamber under both normal and high temperature to investigate the effects of heat stress on morphological, growth and development indexes and physiological and biochemical characteristics during flowering, grain filling stage and the whole growth stage. Identification index of heat resistance during flowering and grain filling stage had been established preliminary. The effects of heat stress on morphological, physiological, biochemical and grain yield had been carried out deep research. This paper adequately expounded and proved the relationship between physiological and biochemical characteristics and heat resistance in rice and discussed the key physiological and biochemical mechanism of heat resistance during grain flowering stage. The results followed:
1. High temperature stress on flowering stage decreased leaves' photosynthesis rate, contents of chlorophyll, increased contents of soluble sugar content, free proline content, malondialdehyde (MDA) content, as well as the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in leaves at flowering stage. Correlation analysis showed that heat susceptibility index significantly or very significantly correlated with relative changed percentage of contents of chlorophyll, free proline, MDA and SOD activity under both high temperature and normal conditions. Therefore, they could be selected as physiological identification indexes for heat tolerance at flowering stage in rice. Using stepwise regression equation to evaluate the heat tolerance of rice cultivars was considered scientifically and reliably.
2. The ability of photosynthesis, osmotic adjustment and antioxidative enzyme activities were effected significantly in the high temperature condition at grain filling stage. The content of chlorophyll, soluble sugar, protein ,MDA, photosynthesis rate and the activity of SOD were correlated with heat resistance on grain filling stage. And heat tolerance on grain filling stage were different because of different cultivars.
3. The content of chlorophyll, soluble sugar, protein, MDA, photosynthesis rate and the activity of SOD are correlative with heat tolerance at grain filling stage, which were synthesized to two new independent comprehensive indices by principal component analysis, Then the comprehensive evaluation of every rice varieties' heat tolerance was obtained by subordinate function value method (SFVM), Consequently, each cultivar' heat tolerance could be evaluated completely and correctly.
4. The high temperature stress in the whole growth stage accelerated rice caducity and loss of protected functions, also lost the activities of antioxidative enzymes in leaves, decreased the osmotic adjustment of leaves, reduced the dry matters production, blocked the translocation and transformation of dry matters in stems and sheaths, decreased yields significantly. In the condition of higher environmental temperature, it should be important to strengthen the farming management in the late growth stage.
引文
[1]王忠.植物生理学.北京,中国农业出版社,1999
[2]吕芝香,赵常青.NaCl对小麦幼苗叶肉细胞超微结构的影响.武汉植物学研究,1990,8(2):122-124
[3]Liu P,Zhao.Effect of cold-hardening on photosynthetic performance and chilling photoinhibition in sweet pepper leaves,Journal of Plant Physiology and Molecular Biology,2002,28(1):51-58
[4]王羽梅,任安详,潘春香.不同浓度NaCl对觅菜幼苗生长、光合速率、离子吸收和甜菜碱含量的影响.植物生理学通讯,2003.39(1):13-16
[5]王洪春.植物抗性生理.植物生理学通讯,1981.17(6):72-81
[6]Blum A.Osmotoc adjustment and growth in barley genotypes under draught stress..Crop Science,1989.29:230-233
[7]Scandalil.J.G.Oxygen stress and superoxide dismutases..Plant Physio,1993.101:7-12
[8]Seel W E,Hendry G A F,Lee J A.Effect of deccication on some activated oxygen processing enzymes and anti-oxidants in mosses.Exp Bot,1992.43:1031-1037
[9]Jacob T.Absicisic acid signal transduction in guard cells is mediated by phospholipase D activity.Proc Natl Acad Sci USA,1999.96(21):12192-12197
[10]赵可夫.盐胁迫下外源ABA对玉米幼苗耐盐性的影响.植物学报:英文版-1995年4月
[11]郑小林,董任瑞.水稻热激反应的研究Ⅰ.幼苗叶片的膜透性和游离脯氨酸的含量变化.湖南农业大学学报,1997,23(2):109-112
[12]张桂莲等.高温胁迫对水稻剑叶保护性酶活性合膜透性的影响.作物学报,2006.32(9):1306-1310.
[13]彭文博,王向阳,赵会杰,等.不同光温条件对小麦旗叶生理特性及粒重的影响.植物生理学通讯,1992,28(6):421-423.
[14]郭培国,李荣华,夜间高温胁迫对水稻叶片光合机构的影响.植物学报,2000,42(7):673-678.
[15]黄英金,罗永锋,黄兴作,等.水稻灌浆期耐热性的品种间差异及其与剑叶光合特性和内源多胺的关系,中国水稻科学,1999,13(4):205-210.
[16]叶陈亮,柯玉琴,陈伟.大白菜耐热性的生理研究Ⅱ.叶片水分和蛋白质代谢与耐热性.福建农业大学学报,1996,25(4):490-493
[17]Hare P D,Cress W A.,Van Staden J.Proline synthesis and degradation:a model system for elucidating stress-related signal transduction.Journal of Experimental Botany,1999,50(333):413-434
[18]Babu R C,Pathan M S,Blum A,et al.Comparison of measurement methods of osmotic adjustment in rice cultivars.Crop Sci,1999,39:150-158
[19]黄英金,张宏玉,漆映雪,等.水稻耐高温逼热的生理机制及育种应用研究初报.科学技术与工程,2004,4(8):655-658.
[20]李稳香,陈立云等.高温条件下杂交中稻结实率与生理生化特性变化的相关性研究.种子,2006.25(5):12-16.
[21]雷东阳,陈立云等.高温对不同杂交稻开花期影响的生理差异.农业现代化研究,2005.26(5):397-400.
[22]黄文江,王纪华,赵春江,等.水稻旱作条件下渗透调节物质和激素含量是关系.干旱地区农业研究,2002,20(1):61-64
[23]汤乔芳,刘云国,曾光明,等.镉胁迫对萝卜幼苗活性氧产生、膜质过氧化和抗氧化酶活性的影响.植物生理与分子生物学学报,2004,30(4):469-474
[24]田小磊,吴晓岚,李云,等.盐胁迫条件下γ-氨基丁酸对玉米幼苗SOD、POD、及CAT 活性的影响.实验生物学报,2005,38(1):75-79
[25]郑小林,董任瑞.水稻热激反应的研究Ⅲ.高温对水稻幼苗叶片过氧化物酶的影响.湖南农业大学学报,1998,24(6):432-435.
[26]曹立勇.水稻耐热性的QTL定位及耐热性与光合速率的相关性.中国水稻科学,2003,17(3):223-227.
[27]曹立勇,朱军,赵松涛,等.水稻籼粳交DH群体耐热性QTLS定位.农业生物技术学报,2002,10(3):210-214.
[28]IRRI.Annual report for 1977-1983.Los Bones,Philippines,1978-1984.
[29]徐云碧,石春海,申宗坦.热害对早稻结实率的影响.浙江农业科学,1989,(2):51-54.
[30]李太贵,沈波.水稻品种开花期抗热性鉴定.作物品种资源,1995(1):34-35.
[31]汤圣祥.闵绍楷.耐逆境育种.中国稻米,1998,(3):38-39.
[32]IRRI.Annual report for 1977-1983.Los Bones,Philippines,1978-1984.
[33]森国谷男.水稻高温胁迫抗性遗传育种研究概况.徐正进译.杂交水稻,1992,(1):47-48.
[34]张宏玉,刘凯等.水稻品种灌浆期耐热性的综合评判.生态学报,2006.26.(7): 2154-2160.
[35]马永战,邹琦,程炳松.小麦的高温伤害与高温适应Ⅱ.高温对麦苗游离脯氯酸的含量的影响.山东农业大学学报,1986,17(4):1-8.
[36]张桂莲,陈立云等.水稻耐热性研究进展.杂交水稻,2005.20(1):1-5.
[37]谭中和.杂交水稻开花期高温危害及其对策的研究.作物学报,1985,11(2):103-108.
[38]上海植物生理研究所.高温对水稻开花结实的影响及其防治.植物学报,1996,18(3):323-329.
[39]朱雪梅,邵继荣,杨文钰,等.温度对杂交中籼稻灌浆期淀粉体发育及胚乳透明度的影响.作物学报,2005,35(6):790-795
[40]IPCC.IPCC Third Assessment Report-Climate Change.http://www.ipcc.ch/,2001
[41]Maestri E,Klueva N,Perrotta C.Molecular genetics of heat tolerance and heat shock proteins in cereals.Plant Mol Biol,2002,48:667-681
[42]IPCC.2007.Climate change 2007:The physical science basis.Summary for policymakers.WMO/UNEP,Paris.
[43]黄义德,曹流俭,武立权,等.2003年安徽省中稻花期高温热害的凋查与分析.安徽农业大学学报,2004,31(4):385-388
[44]汤昌本,林迢,简根梅,等.浙江早稻高温危害研究.浙江气象技,2000,21(2):14-18
[45]Yoshida S,Satake T,Machill D S.High temperature stress in rice.IRRI Research Paper Series,1981,67
[46]曾汉来,卢开阳,贺道华,等.中籼杂交水稻新组合结实性的高温适应性鉴定.华中农业大学学报,2000,19(1):1-4.
[47]王锋尖,黄英金.水稻高温胁迫及耐热性育种.中国农学通报,2004,20(3):87-90
[48]徐海波,王光明,隗溟,等.高温胁迫下水稻花粉粒性状与结实率的相关分析.西南农业大学学报,2001,23(3):205-207
[49]欧志英,林桂珠,彭长连.超高产杂交水稻培矮64S/E32和两优培九剑叶对高温的响应.中国水稻科学,2005,19(3):249-254
[50]李合生主编.植物生理生化试验原理和技术.北京,高等教育出版社,2000
[51]任红旭,陈雄,吴冬秀,等.CO_2浓度升高对干旱胁迫下蚕豆光合作用和抗氧化能力的影响.作物学报,2001,27(6):729-736.
[52]周广生,梅方竹,周竹青,等.小麦不同品种耐湿性生理指标综合评价及预测.中国农业科学,2003,36(11):1378-1382
[53]郝黎仁,樊元,郝哲欧,等.SPSS实用统计分析.北京,中国水利水电出版社,2003
[54]任昌福,陈安和,刘保国.高温影响杂交水稻开花结实的生理生化基础.西南农业大 学学报,1990,12(5):440-43.
[55]肖浪涛,王三根主编.植物生理学.北京:中国农业出版社,2004.
[56]Berry J,Bjorknmn O.Photosynthetic response and adaptation to temperature in higher plants.Ann Rev Plant Physiol,1980,31:491-543.
[57]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响.生态学报,2002,22(4):503-507.
[58]彭文博,王向刚,赵会杰,等.不同光温条件对小麦旗叶生理特性及粒重的影响,植物生理学通讯,1992,28(6):421-423.
[59]王孝威,曹慧.高等植物衰老的机理研究.山西农业大学学报,2004,24(4):416-419.
[60]杜秀敏,殷文璇,赵彦修,等.植物中活性氧的产生及清除机制.生物工程学报,2001,17(2):121-125.
[61]许长成,邹琦.大豆叶片旱促衰老及其与膜脂过氧化的关系.作物学报,1993,19(4):359-364.
[62]罗利军,张启发.栽培稻抗旱性研究的现状与策略.中国水稻科学,2001,15(3):209-214
[63]龚明.作物抗旱性鉴定方法与指标及其综合评价,云南农业大学学报,1989,4(1):73-80
[64]钮福祥,华希新,郭小丁,等.甘薯品种抗旱性生理指标及其综合评价初探,作物学报,1996,22(4):392-398
[65]西山岩男,赵贵彬,凌天行.水稻高温障碍的研究.国外农学-水稻,1982,(5):17-20.
[66]杨纯明,James L,Heilman.短期高温对水稻生长发育和产量的影响.谢国禄译.国外作物育种,1994,(2):4-5.
[67]张磊,吴冬云,朱碧岩.灌浆期不同温光对水稻叶、籽粒可溶性蛋白质及可溶性糖动态变化的影响.华南师范大学学报(自然科学版),2002(2):98-101.
[68]汤日圣,郑建初,陈留根.高温对杂交水稻籽粒灌浆和剑叶某些生理特性的影响.植物生理与分子生物学学报,2005,31(6):657-662
[2]吕芝香,赵常青.NaCl对小麦幼苗叶肉细胞超微结构的影响.武汉植物学研究,1990,8(2):122-124
[3]Liu P,Zhao.Effect of cold-hardening on photosynthetic performance and chilling photoinhibition in sweet pepper leaves,Journal of Plant Physiology and Molecular Biology,2002,28(1):51-58
[4]王羽梅,任安详,潘春香.不同浓度NaCl对觅菜幼苗生长、光合速率、离子吸收和甜菜碱含量的影响.植物生理学通讯,2003.39(1):13-16
[5]王洪春.植物抗性生理.植物生理学通讯,1981.17(6):72-81
[6]Blum A.Osmotoc adjustment and growth in barley genotypes under draught stress..Crop Science,1989.29:230-233
[7]Scandalil.J.G.Oxygen stress and superoxide dismutases..Plant Physio,1993.101:7-12
[8]Seel W E,Hendry G A F,Lee J A.Effect of deccication on some activated oxygen processing enzymes and anti-oxidants in mosses.Exp Bot,1992.43:1031-1037
[9]Jacob T.Absicisic acid signal transduction in guard cells is mediated by phospholipase D activity.Proc Natl Acad Sci USA,1999.96(21):12192-12197
[10]赵可夫.盐胁迫下外源ABA对玉米幼苗耐盐性的影响.植物学报:英文版-1995年4月
[11]郑小林,董任瑞.水稻热激反应的研究Ⅰ.幼苗叶片的膜透性和游离脯氨酸的含量变化.湖南农业大学学报,1997,23(2):109-112
[12]张桂莲等.高温胁迫对水稻剑叶保护性酶活性合膜透性的影响.作物学报,2006.32(9):1306-1310.
[13]彭文博,王向阳,赵会杰,等.不同光温条件对小麦旗叶生理特性及粒重的影响.植物生理学通讯,1992,28(6):421-423.
[14]郭培国,李荣华,夜间高温胁迫对水稻叶片光合机构的影响.植物学报,2000,42(7):673-678.
[15]黄英金,罗永锋,黄兴作,等.水稻灌浆期耐热性的品种间差异及其与剑叶光合特性和内源多胺的关系,中国水稻科学,1999,13(4):205-210.
[16]叶陈亮,柯玉琴,陈伟.大白菜耐热性的生理研究Ⅱ.叶片水分和蛋白质代谢与耐热性.福建农业大学学报,1996,25(4):490-493
[17]Hare P D,Cress W A.,Van Staden J.Proline synthesis and degradation:a model system for elucidating stress-related signal transduction.Journal of Experimental Botany,1999,50(333):413-434
[18]Babu R C,Pathan M S,Blum A,et al.Comparison of measurement methods of osmotic adjustment in rice cultivars.Crop Sci,1999,39:150-158
[19]黄英金,张宏玉,漆映雪,等.水稻耐高温逼热的生理机制及育种应用研究初报.科学技术与工程,2004,4(8):655-658.
[20]李稳香,陈立云等.高温条件下杂交中稻结实率与生理生化特性变化的相关性研究.种子,2006.25(5):12-16.
[21]雷东阳,陈立云等.高温对不同杂交稻开花期影响的生理差异.农业现代化研究,2005.26(5):397-400.
[22]黄文江,王纪华,赵春江,等.水稻旱作条件下渗透调节物质和激素含量是关系.干旱地区农业研究,2002,20(1):61-64
[23]汤乔芳,刘云国,曾光明,等.镉胁迫对萝卜幼苗活性氧产生、膜质过氧化和抗氧化酶活性的影响.植物生理与分子生物学学报,2004,30(4):469-474
[24]田小磊,吴晓岚,李云,等.盐胁迫条件下γ-氨基丁酸对玉米幼苗SOD、POD、及CAT 活性的影响.实验生物学报,2005,38(1):75-79
[25]郑小林,董任瑞.水稻热激反应的研究Ⅲ.高温对水稻幼苗叶片过氧化物酶的影响.湖南农业大学学报,1998,24(6):432-435.
[26]曹立勇.水稻耐热性的QTL定位及耐热性与光合速率的相关性.中国水稻科学,2003,17(3):223-227.
[27]曹立勇,朱军,赵松涛,等.水稻籼粳交DH群体耐热性QTLS定位.农业生物技术学报,2002,10(3):210-214.
[28]IRRI.Annual report for 1977-1983.Los Bones,Philippines,1978-1984.
[29]徐云碧,石春海,申宗坦.热害对早稻结实率的影响.浙江农业科学,1989,(2):51-54.
[30]李太贵,沈波.水稻品种开花期抗热性鉴定.作物品种资源,1995(1):34-35.
[31]汤圣祥.闵绍楷.耐逆境育种.中国稻米,1998,(3):38-39.
[32]IRRI.Annual report for 1977-1983.Los Bones,Philippines,1978-1984.
[33]森国谷男.水稻高温胁迫抗性遗传育种研究概况.徐正进译.杂交水稻,1992,(1):47-48.
[34]张宏玉,刘凯等.水稻品种灌浆期耐热性的综合评判.生态学报,2006.26.(7): 2154-2160.
[35]马永战,邹琦,程炳松.小麦的高温伤害与高温适应Ⅱ.高温对麦苗游离脯氯酸的含量的影响.山东农业大学学报,1986,17(4):1-8.
[36]张桂莲,陈立云等.水稻耐热性研究进展.杂交水稻,2005.20(1):1-5.
[37]谭中和.杂交水稻开花期高温危害及其对策的研究.作物学报,1985,11(2):103-108.
[38]上海植物生理研究所.高温对水稻开花结实的影响及其防治.植物学报,1996,18(3):323-329.
[39]朱雪梅,邵继荣,杨文钰,等.温度对杂交中籼稻灌浆期淀粉体发育及胚乳透明度的影响.作物学报,2005,35(6):790-795
[40]IPCC.IPCC Third Assessment Report-Climate Change.http://www.ipcc.ch/,2001
[41]Maestri E,Klueva N,Perrotta C.Molecular genetics of heat tolerance and heat shock proteins in cereals.Plant Mol Biol,2002,48:667-681
[42]IPCC.2007.Climate change 2007:The physical science basis.Summary for policymakers.WMO/UNEP,Paris.
[43]黄义德,曹流俭,武立权,等.2003年安徽省中稻花期高温热害的凋查与分析.安徽农业大学学报,2004,31(4):385-388
[44]汤昌本,林迢,简根梅,等.浙江早稻高温危害研究.浙江气象技,2000,21(2):14-18
[45]Yoshida S,Satake T,Machill D S.High temperature stress in rice.IRRI Research Paper Series,1981,67
[46]曾汉来,卢开阳,贺道华,等.中籼杂交水稻新组合结实性的高温适应性鉴定.华中农业大学学报,2000,19(1):1-4.
[47]王锋尖,黄英金.水稻高温胁迫及耐热性育种.中国农学通报,2004,20(3):87-90
[48]徐海波,王光明,隗溟,等.高温胁迫下水稻花粉粒性状与结实率的相关分析.西南农业大学学报,2001,23(3):205-207
[49]欧志英,林桂珠,彭长连.超高产杂交水稻培矮64S/E32和两优培九剑叶对高温的响应.中国水稻科学,2005,19(3):249-254
[50]李合生主编.植物生理生化试验原理和技术.北京,高等教育出版社,2000
[51]任红旭,陈雄,吴冬秀,等.CO_2浓度升高对干旱胁迫下蚕豆光合作用和抗氧化能力的影响.作物学报,2001,27(6):729-736.
[52]周广生,梅方竹,周竹青,等.小麦不同品种耐湿性生理指标综合评价及预测.中国农业科学,2003,36(11):1378-1382
[53]郝黎仁,樊元,郝哲欧,等.SPSS实用统计分析.北京,中国水利水电出版社,2003
[54]任昌福,陈安和,刘保国.高温影响杂交水稻开花结实的生理生化基础.西南农业大 学学报,1990,12(5):440-43.
[55]肖浪涛,王三根主编.植物生理学.北京:中国农业出版社,2004.
[56]Berry J,Bjorknmn O.Photosynthetic response and adaptation to temperature in higher plants.Ann Rev Plant Physiol,1980,31:491-543.
[57]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响.生态学报,2002,22(4):503-507.
[58]彭文博,王向刚,赵会杰,等.不同光温条件对小麦旗叶生理特性及粒重的影响,植物生理学通讯,1992,28(6):421-423.
[59]王孝威,曹慧.高等植物衰老的机理研究.山西农业大学学报,2004,24(4):416-419.
[60]杜秀敏,殷文璇,赵彦修,等.植物中活性氧的产生及清除机制.生物工程学报,2001,17(2):121-125.
[61]许长成,邹琦.大豆叶片旱促衰老及其与膜脂过氧化的关系.作物学报,1993,19(4):359-364.
[62]罗利军,张启发.栽培稻抗旱性研究的现状与策略.中国水稻科学,2001,15(3):209-214
[63]龚明.作物抗旱性鉴定方法与指标及其综合评价,云南农业大学学报,1989,4(1):73-80
[64]钮福祥,华希新,郭小丁,等.甘薯品种抗旱性生理指标及其综合评价初探,作物学报,1996,22(4):392-398
[65]西山岩男,赵贵彬,凌天行.水稻高温障碍的研究.国外农学-水稻,1982,(5):17-20.
[66]杨纯明,James L,Heilman.短期高温对水稻生长发育和产量的影响.谢国禄译.国外作物育种,1994,(2):4-5.
[67]张磊,吴冬云,朱碧岩.灌浆期不同温光对水稻叶、籽粒可溶性蛋白质及可溶性糖动态变化的影响.华南师范大学学报(自然科学版),2002(2):98-101.
[68]汤日圣,郑建初,陈留根.高温对杂交水稻籽粒灌浆和剑叶某些生理特性的影响.植物生理与分子生物学学报,2005,31(6):657-662
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |