开胃进食汤超微配方颗粒对脾虚证胃肠激素调节作用的研究
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摘要
第一部分开胃进食汤超微配方颗粒治疗脾虚证的临床研究
目的:评价开胃进食汤超微配方颗粒治疗脾虚证的临床疗效,观察其对胃肠激素的影响。
方法:采用随机对照实验方法对90例辨证为脾虚证患者分为3组,分别予开胃进食汤超微配方颗粒等倍剂量、1/2剂量、健胃消食片治疗,以2周为一疗程,观察各组治疗前后临床症状变化及血浆GAS、SST、MTL水平。
结果:1.开胃进食汤超微等倍组总有效率为86.67%,1/2剂量组为83.33%,两者临床疗效基本相当,显效率均较健胃消食片组为优(P＜0.05)。2与治疗前比较,开胃进食汤超微等倍组、超微1/2组、健胃消食片组均能升高血浆GAS、MTL水平,降低SST水平,组间比较差异无统计学意义。
结论:开胃进食汤超微配方颗粒能明显改善脾虚患者临床症状,1/2剂量组与等倍组疗效基本相当;开胃进食汤超微配方颗粒能明显改善脾虚患者胃肠激素水平,使之趋于正常。
第二部分开胃进食汤超微配方颗粒对大鼠胃液分泌的影响
目的:研究开胃进食汤超微配方颗粒对正常大鼠及利血平所致脾虚证大鼠胃液分泌的影响
方法:采用完全随机对照设计方案,选用健胃消食片作阳性药物对照,观察开胃进食汤超微配方颗粒等倍剂量、1/2剂量、1/4剂量、传统汤剂对正常SD大鼠胃液分泌的影响,找出最佳有效剂量,在此基础上进一步观察开胃进食汤对利血平所致脾虚大鼠胃液分泌的影响。
结果:1与正常对照组比较,健胃消食片组、开胃进食汤传统汤剂组和超微配方颗粒各剂量组均能增加正常大鼠的胃液分泌量,降低胃液PH值,使胃液中游离酸、总酸度及总酸排出量明显增加,能明显增加胃蛋白酶活性;在增加幅度方面,超微1/2组与超微等倍组作用基本相当,优于超微1/4组(P＜0.05),亦较传统汤剂组、健胃消食片组为强,但无统计学意义。2.与脾虚模型组比较,健胃消食片组、开胃进食汤传统汤剂组和超微1/2组的胃液分泌量,游离酸、总酸度及总酸排出量均减少,胃蛋白酶活性降低,PH值升高;且超微1/2组较健胃消食片组、传统汤剂组减少明显,但亦无统计学意义。
结论:开胃进食汤超微配方颗粒对大鼠胃液分泌具有双向作用。1.促进正常大鼠胃液和胃酸的分泌,增加胃蛋白酶活性,且促进作用随剂量的增加有所增强,以1/2剂量为最佳;2.抑制利血平所致脾虚大鼠胃液和胃酸的分泌,降低胃蛋白酶活性。
第三部分开胃进食汤超微配方颗粒对脾虚证大鼠组织和血浆GAS、SST、MTL含量的影响
目的:观察开胃进食汤微配方颗粒对正常大鼠及利血平所致脾虚证大鼠组织和血浆GAS、SST、MTL含量的影响。
方法:取SD大鼠60只,随机分为正常对照组、正常超微组、脾虚对照组、脾虚超微组、传统汤剂组、健胃消食片组,用利血平造成脾虚模型,正常组用生理盐水造模,然后分别予生理盐水、开胃进食汤超微配方颗粒1/2剂量混悬液、健胃消食片溶液灌胃,2周后提取标本,用放射免疫法(RIA)检测各组相关组织、血浆GAS、SST、MTL水平。
结果:1.正常超微组组织、血浆GAS、MTL水平显著高于正常对照组(P＜0.01),SST水平低于对照组(P＜0.05);2.脾虚超微组、传统组、健胃消食片组组织、血浆GAS水平均显著低于脾虚模型组,SST、MTL水平则显著高于脾虚模型组;3.组间比较,脾虚超微组组织、血浆GAS水平低于传统汤剂组和健胃消食片组,SST、MTL水平高于传统汤剂组和健胃消食片组,与健胃消食片组比较P＜0.05。
结论:开胃进食汤超微配方颗粒对大鼠胃肠激素的调节呈现双向作用。
1.刺激正常大鼠分泌GAS、MTL,抑制SST的分泌;2.能升高利血平所致脾虚大鼠组织、血浆SST、MTL水平,降低组织、血浆GAS水平。表明开胃进食汤能调节脾虚大鼠之胃肠激素紊乱,使之趋向正常。
第四部分开胃进食汤超微配方颗粒对大鼠胃窦粘膜GAS、SSTR1mRNA表达的调节
目的:研究开胃进食汤超微配方颗粒对正常大鼠及脾虚证大鼠胃窦粘膜GAS、SSTR1mRNA表达的影响,探讨其调节相关胃肠激素水平的分子机制。
方法:在第三部分留取标本的基础上,运用RT-PCR方法测定各组胃窦粘膜GASmRNA、SSTR1mRNA的表达。
结果:1.开胃进食汤超微配方颗粒可上调GASmRNA在正常大鼠胃粘膜的表达水平,而减弱其在脾虚大鼠胃粘膜的表达;2.开胃进食汤超微配方颗粒可减弱SSTR1mRNA在正常大鼠胃粘膜的表达水平,上调其在脾虚大鼠胃粘膜的表达。
结论:开胃进食汤超微配方颗粒通过上调正常大鼠胃粘膜GASmRNA的表达、减弱SSTR1mRNA的表达,以及通过上调脾虚大鼠胃粘膜SSTR1mRNA的表达、减弱GAsmRNA的表达来调节胃肠激素的分泌,进而调节胃液、胃酸的分泌,在一定程度从分子水平上阐述了开胃进食汤通过调节胃肠激素治疗脾虚证的机制。
PartⅠClinic research on Spleen deficiency treated by Kaiwei Jinshitang ultramicro-drug piece
Objective:To evaluate the clinical effect of Kaiwei Jinshitang(KWJST) ultramicro-drug piece(UMDP) on Spleen deficiency,and the gastrointestinal hormone changes.
Method:90 patients with Spleen deficiency were divided randomly into three groups,treated with one dose and half dose of KWJST UMDP and Jianwei Xiaoshi Tablet(JWXST) respectively,two weeks for one period of treatment,the serum gastrin(GAS),somatostatin(SST) and motilin(MTL) were selected as evaluating items.
Results:1.The total effect rate of one dose and half dose of KWJST UMDP were 86.67%and 83.33%respectively and the clinic efficacy were equal.The effective rate of KWJST UMDP was higher than that of JWXST(P＜0.05).2.One dose and half dose of KWJST UMDP and JWXST could raise serum GAS and MTL,while reduce SST,and there were no significant difference among them.
Conclusion:KWJST UMDP could improve the symptom of Spleen deficiency, the half dose group equals to one dose group.KWJS UMDP could prompt GAS and MTL,as well inhibit SST secretion.
PartⅡThe effect of KWJST UMDP on rats' gastric juice secretion
Objective:To study the effect of KWJST UMDP on the gastric juice secretion in normal rats and Spleen deficiency rats induced by reserpine.
Method:In order to find out the best dose,random comparing design applied, and JWXST selected as positive contrast drug,one dose,half dose and one fourth dose of KWJST UMDP,and traditional decoction of KWJST respectively were used to observe the effect of the above materials on the normal SD rats' gastric juice secretion.And the effect of KWJST UMDP on the gastric juice secretion in Spleen deficiency rats induced by reserpine was also evaluated.
Results:1.Comparing to normal group,JWXST and traditional decoction and different doses UMDP of KWJST could increase rats gastric juice secretion, decreasing gastric juice PH value,increasing free acid and total acidity and output of total acid,as well pepsin activity.As for the increasing amplitude,half dose of KWJST UMDP equaled to one dose,and was higher than one fourth dose(P＜0.05). While there were no significant difference among traditional decoction and half dose of KWJST UMDP and JWXST.2.Comparing to Spleen deficiency group, the gastric juice,free acid,total acidity and output of acid were lower in JWXST, traditional decoction and half dose of KWJST,as well as pepsin activity,while the higher PH value in later three groups.And for the above items,there were no significant difference among the later three groups.
Conclusion:KWJST UMDP has double effects on rats' gastric juice secretion.1. It could promote normal rats' gastric juice and acid secretion,and increase pepsin activity.With the higher dose,the higher effects appear,and half of dose has best effects.2.It could also inhibit gastric juice and acid secretion and pepsin activity in Spleen deficiency rats induced by reserpine.
PartⅢThe effect of KWJST UMDP on Spleen deficiency rats' GAS,SST and MTL content in tissue and serum respectively
Objective:To explore the effect of KWJST UMDP on GAS,SST and MTL content in tissue and serum of normal rats and Spleen deficiency rats induced by reserpine.
Method:60 SD rats were randomly divided into normal contrast group,normal group with KWJST UMDP,Spleen deficiency group,Spleen deficiency treated with KWJST UMDP,traditional decoction group,JWXST group.Spleen deficiency group were induced by reserpine,and normal contrast group with normal saline.Then normal saline,half dose of KWJST UMDP and JWXST intragastric administration for two weeks,the tissue and serum GAS,SST and MTL were assayed respectively by radioimmunoassay(RIA).
Results:1.The tissue and serum GAS and MTL in the normal group with KWJST UMDP were higher than those in normal contrast group significantly (P＜0.01),while the SST was lower in the normal group with KWJST UMDP(P＜0.05).2.Both of the tissue and serum GAS in Spleen deficiency treated with KWJST UMDP,traditional decoction of KWJST and JWXST,were lower than those in Spleen deficiency group,while SST and MTL were higher than those in Spleen deficiency group.3.Among the groups,the tissue and serum GAS were lower in Spleen deficiency treated with KWJST UMDP than those in traditional decoction of KWJST and JWXST,while the SST and MTL were higher.
Conclusion:KWJST UMDP has double regulating function for rats' gastrointestinal hormone.1 It could stimulate normal rats with more GAS and MTL secretion,inhibiting SST secretion.2 It could raise the tissue and serum SST and MTL and reduce GAS level in the Spleen deficiency rats induced by reserpine. It shows that KWJST UMDP could regulate the inordinate gastrointestinal hormone for the normal level.
PartⅣThe regulation of KWJST UMDP on normal rats and Spleen deficiency rats' GAS mRNA and SSTR mRNA expression in sinuses ventriculi mucosa
Objective:To observe the effect of KWJST UMDP on normal and Spleen deficiency rats' GAS and SSTR mRNA expression in sinuses ventriculi mucosa, and explore the molecular mechanism of regulating gastrointestinal hormone.
Method:RT-PCR was used for GAS mRNA and SSTR mRNA expression in rats' sinuses ventriculi mucosa.
Results:1.KWJST UMDP could up-regulate GAS mRNA expression in sinuses ventriculi mucosa of the normal rats,while weakening its expression in the Spleen deficiency rats.2.KWJST UMDP could weaken SSTR mRNA expression in sinuses ventriculi mucosa of the normal rats,while up-regulating its expression in the Spleen deficiency rats
Conclusion:KWJST UMDP could regulate gastrointestinal hormone by way of up-regulating GAS mRNA and down-regulating SSTR mRNA expression in normal rat,up-regulating SSTR mRNA and down-regulating SSTR mRNA expression in Spleen deficiency rat.And it is the base for regulating gastric juice and acid secretion,which is the molecular mechanism of KWJST UMDP treating Spleen deficiency in a way.
目的:评价开胃进食汤超微配方颗粒治疗脾虚证的临床疗效,观察其对胃肠激素的影响。
方法:采用随机对照实验方法对90例辨证为脾虚证患者分为3组,分别予开胃进食汤超微配方颗粒等倍剂量、1/2剂量、健胃消食片治疗,以2周为一疗程,观察各组治疗前后临床症状变化及血浆GAS、SST、MTL水平。
结果:1.开胃进食汤超微等倍组总有效率为86.67%,1/2剂量组为83.33%,两者临床疗效基本相当,显效率均较健胃消食片组为优(P＜0.05)。2与治疗前比较,开胃进食汤超微等倍组、超微1/2组、健胃消食片组均能升高血浆GAS、MTL水平,降低SST水平,组间比较差异无统计学意义。
结论:开胃进食汤超微配方颗粒能明显改善脾虚患者临床症状,1/2剂量组与等倍组疗效基本相当;开胃进食汤超微配方颗粒能明显改善脾虚患者胃肠激素水平,使之趋于正常。
第二部分开胃进食汤超微配方颗粒对大鼠胃液分泌的影响
目的:研究开胃进食汤超微配方颗粒对正常大鼠及利血平所致脾虚证大鼠胃液分泌的影响
方法:采用完全随机对照设计方案,选用健胃消食片作阳性药物对照,观察开胃进食汤超微配方颗粒等倍剂量、1/2剂量、1/4剂量、传统汤剂对正常SD大鼠胃液分泌的影响,找出最佳有效剂量,在此基础上进一步观察开胃进食汤对利血平所致脾虚大鼠胃液分泌的影响。
结果:1与正常对照组比较,健胃消食片组、开胃进食汤传统汤剂组和超微配方颗粒各剂量组均能增加正常大鼠的胃液分泌量,降低胃液PH值,使胃液中游离酸、总酸度及总酸排出量明显增加,能明显增加胃蛋白酶活性;在增加幅度方面,超微1/2组与超微等倍组作用基本相当,优于超微1/4组(P＜0.05),亦较传统汤剂组、健胃消食片组为强,但无统计学意义。2.与脾虚模型组比较,健胃消食片组、开胃进食汤传统汤剂组和超微1/2组的胃液分泌量,游离酸、总酸度及总酸排出量均减少,胃蛋白酶活性降低,PH值升高;且超微1/2组较健胃消食片组、传统汤剂组减少明显,但亦无统计学意义。
结论:开胃进食汤超微配方颗粒对大鼠胃液分泌具有双向作用。1.促进正常大鼠胃液和胃酸的分泌,增加胃蛋白酶活性,且促进作用随剂量的增加有所增强,以1/2剂量为最佳;2.抑制利血平所致脾虚大鼠胃液和胃酸的分泌,降低胃蛋白酶活性。
第三部分开胃进食汤超微配方颗粒对脾虚证大鼠组织和血浆GAS、SST、MTL含量的影响
目的:观察开胃进食汤微配方颗粒对正常大鼠及利血平所致脾虚证大鼠组织和血浆GAS、SST、MTL含量的影响。
方法:取SD大鼠60只,随机分为正常对照组、正常超微组、脾虚对照组、脾虚超微组、传统汤剂组、健胃消食片组,用利血平造成脾虚模型,正常组用生理盐水造模,然后分别予生理盐水、开胃进食汤超微配方颗粒1/2剂量混悬液、健胃消食片溶液灌胃,2周后提取标本,用放射免疫法(RIA)检测各组相关组织、血浆GAS、SST、MTL水平。
结果:1.正常超微组组织、血浆GAS、MTL水平显著高于正常对照组(P＜0.01),SST水平低于对照组(P＜0.05);2.脾虚超微组、传统组、健胃消食片组组织、血浆GAS水平均显著低于脾虚模型组,SST、MTL水平则显著高于脾虚模型组;3.组间比较,脾虚超微组组织、血浆GAS水平低于传统汤剂组和健胃消食片组,SST、MTL水平高于传统汤剂组和健胃消食片组,与健胃消食片组比较P＜0.05。
结论:开胃进食汤超微配方颗粒对大鼠胃肠激素的调节呈现双向作用。
1.刺激正常大鼠分泌GAS、MTL,抑制SST的分泌;2.能升高利血平所致脾虚大鼠组织、血浆SST、MTL水平,降低组织、血浆GAS水平。表明开胃进食汤能调节脾虚大鼠之胃肠激素紊乱,使之趋向正常。
第四部分开胃进食汤超微配方颗粒对大鼠胃窦粘膜GAS、SSTR1mRNA表达的调节
目的:研究开胃进食汤超微配方颗粒对正常大鼠及脾虚证大鼠胃窦粘膜GAS、SSTR1mRNA表达的影响,探讨其调节相关胃肠激素水平的分子机制。
方法:在第三部分留取标本的基础上,运用RT-PCR方法测定各组胃窦粘膜GASmRNA、SSTR1mRNA的表达。
结果:1.开胃进食汤超微配方颗粒可上调GASmRNA在正常大鼠胃粘膜的表达水平,而减弱其在脾虚大鼠胃粘膜的表达;2.开胃进食汤超微配方颗粒可减弱SSTR1mRNA在正常大鼠胃粘膜的表达水平,上调其在脾虚大鼠胃粘膜的表达。
结论:开胃进食汤超微配方颗粒通过上调正常大鼠胃粘膜GASmRNA的表达、减弱SSTR1mRNA的表达,以及通过上调脾虚大鼠胃粘膜SSTR1mRNA的表达、减弱GAsmRNA的表达来调节胃肠激素的分泌,进而调节胃液、胃酸的分泌,在一定程度从分子水平上阐述了开胃进食汤通过调节胃肠激素治疗脾虚证的机制。
PartⅠClinic research on Spleen deficiency treated by Kaiwei Jinshitang ultramicro-drug piece
Objective:To evaluate the clinical effect of Kaiwei Jinshitang(KWJST) ultramicro-drug piece(UMDP) on Spleen deficiency,and the gastrointestinal hormone changes.
Method:90 patients with Spleen deficiency were divided randomly into three groups,treated with one dose and half dose of KWJST UMDP and Jianwei Xiaoshi Tablet(JWXST) respectively,two weeks for one period of treatment,the serum gastrin(GAS),somatostatin(SST) and motilin(MTL) were selected as evaluating items.
Results:1.The total effect rate of one dose and half dose of KWJST UMDP were 86.67%and 83.33%respectively and the clinic efficacy were equal.The effective rate of KWJST UMDP was higher than that of JWXST(P＜0.05).2.One dose and half dose of KWJST UMDP and JWXST could raise serum GAS and MTL,while reduce SST,and there were no significant difference among them.
Conclusion:KWJST UMDP could improve the symptom of Spleen deficiency, the half dose group equals to one dose group.KWJS UMDP could prompt GAS and MTL,as well inhibit SST secretion.
PartⅡThe effect of KWJST UMDP on rats' gastric juice secretion
Objective:To study the effect of KWJST UMDP on the gastric juice secretion in normal rats and Spleen deficiency rats induced by reserpine.
Method:In order to find out the best dose,random comparing design applied, and JWXST selected as positive contrast drug,one dose,half dose and one fourth dose of KWJST UMDP,and traditional decoction of KWJST respectively were used to observe the effect of the above materials on the normal SD rats' gastric juice secretion.And the effect of KWJST UMDP on the gastric juice secretion in Spleen deficiency rats induced by reserpine was also evaluated.
Results:1.Comparing to normal group,JWXST and traditional decoction and different doses UMDP of KWJST could increase rats gastric juice secretion, decreasing gastric juice PH value,increasing free acid and total acidity and output of total acid,as well pepsin activity.As for the increasing amplitude,half dose of KWJST UMDP equaled to one dose,and was higher than one fourth dose(P＜0.05). While there were no significant difference among traditional decoction and half dose of KWJST UMDP and JWXST.2.Comparing to Spleen deficiency group, the gastric juice,free acid,total acidity and output of acid were lower in JWXST, traditional decoction and half dose of KWJST,as well as pepsin activity,while the higher PH value in later three groups.And for the above items,there were no significant difference among the later three groups.
Conclusion:KWJST UMDP has double effects on rats' gastric juice secretion.1. It could promote normal rats' gastric juice and acid secretion,and increase pepsin activity.With the higher dose,the higher effects appear,and half of dose has best effects.2.It could also inhibit gastric juice and acid secretion and pepsin activity in Spleen deficiency rats induced by reserpine.
PartⅢThe effect of KWJST UMDP on Spleen deficiency rats' GAS,SST and MTL content in tissue and serum respectively
Objective:To explore the effect of KWJST UMDP on GAS,SST and MTL content in tissue and serum of normal rats and Spleen deficiency rats induced by reserpine.
Method:60 SD rats were randomly divided into normal contrast group,normal group with KWJST UMDP,Spleen deficiency group,Spleen deficiency treated with KWJST UMDP,traditional decoction group,JWXST group.Spleen deficiency group were induced by reserpine,and normal contrast group with normal saline.Then normal saline,half dose of KWJST UMDP and JWXST intragastric administration for two weeks,the tissue and serum GAS,SST and MTL were assayed respectively by radioimmunoassay(RIA).
Results:1.The tissue and serum GAS and MTL in the normal group with KWJST UMDP were higher than those in normal contrast group significantly (P＜0.01),while the SST was lower in the normal group with KWJST UMDP(P＜0.05).2.Both of the tissue and serum GAS in Spleen deficiency treated with KWJST UMDP,traditional decoction of KWJST and JWXST,were lower than those in Spleen deficiency group,while SST and MTL were higher than those in Spleen deficiency group.3.Among the groups,the tissue and serum GAS were lower in Spleen deficiency treated with KWJST UMDP than those in traditional decoction of KWJST and JWXST,while the SST and MTL were higher.
Conclusion:KWJST UMDP has double regulating function for rats' gastrointestinal hormone.1 It could stimulate normal rats with more GAS and MTL secretion,inhibiting SST secretion.2 It could raise the tissue and serum SST and MTL and reduce GAS level in the Spleen deficiency rats induced by reserpine. It shows that KWJST UMDP could regulate the inordinate gastrointestinal hormone for the normal level.
PartⅣThe regulation of KWJST UMDP on normal rats and Spleen deficiency rats' GAS mRNA and SSTR mRNA expression in sinuses ventriculi mucosa
Objective:To observe the effect of KWJST UMDP on normal and Spleen deficiency rats' GAS and SSTR mRNA expression in sinuses ventriculi mucosa, and explore the molecular mechanism of regulating gastrointestinal hormone.
Method:RT-PCR was used for GAS mRNA and SSTR mRNA expression in rats' sinuses ventriculi mucosa.
Results:1.KWJST UMDP could up-regulate GAS mRNA expression in sinuses ventriculi mucosa of the normal rats,while weakening its expression in the Spleen deficiency rats.2.KWJST UMDP could weaken SSTR mRNA expression in sinuses ventriculi mucosa of the normal rats,while up-regulating its expression in the Spleen deficiency rats
Conclusion:KWJST UMDP could regulate gastrointestinal hormone by way of up-regulating GAS mRNA and down-regulating SSTR mRNA expression in normal rat,up-regulating SSTR mRNA and down-regulating SSTR mRNA expression in Spleen deficiency rat.And it is the base for regulating gastric juice and acid secretion,which is the molecular mechanism of KWJST UMDP treating Spleen deficiency in a way.
引文
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[1]金敬善,王广才,张绳组,等.血清中胃泌素水平与脾虚证的关系 中西医结合杂志,1982,2(1)25-26
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[3]宋开源,刘旭光,汪开明,等.辰、戊时辰四君子汤治疗“脾虚证”大鼠血清胃泌素昼夜节律性的影响[J].成都中医药大学学报,1996,19(1):32-35
[4]牟德峻.消化性溃疡中医辨证分型与胃酸、胃泌素、环核苷酸关系的探讨[J]中西医结合杂志,1988,8(9):531
[5]熊洪翔.中医辨证分型对慢性萎缩性胃炎患者空腹血清胃泌素变化的观察[J].福建中医,1983,14(2):50
[6]涂福音.慢性萎缩性胃炎103例中医辨证分型与胃泌素、尿胃蛋白酶关系探讨[J]福建中医药,1990,21(2):11
[7]张万岱.脾虚证患者胃液及胃窦十二指肠黏膜胃泌素含量的研究[J].新消化病学杂志,1993,1(特1):14
[8]张桂珍.脾虚证患者胃窦G细胞体视学定量分析[J].中西医结合杂志,1989,9(12):711
[9]智发朝,张万岱,宋于刚.生长抑素与脾虚证关系的研究.新消化病学杂志,1993;1(特1)::49-50
[10]马建伟,徐丽梅,郝刚,等.脾虚证与血浆和胃窦组织中生长抑素关系的探讨.实用中西医结合杂志,1995,8(11):644-645
[11]宋于刚,姚永莉,张万岱.脾虚证与体液胃泌素及生长抑素关系的实验研究.胃肠病学,1998,3(3):182-183
[12]张万岱,姚永莉,宋于刚,实验脾虚证组织中胃泌素及生长抑素含量变化及其意义.胃肠病学,1998,3(3):180-181
[13]任平,夏天,张忠兵,等.脾虚泄泻与血浆及肠组织中胃动素的关系 第四军医大学学报,1991,12(5):391-392
[14]任平,宋国增,夏天,等.对脾虚泄泻患者胃动素及前列腺素的初探 中国医药学报,1993;8(1):22-24
[15]任平,黄熙,蒋永培,等.四君子汤对脾虚大鼠胃动素及川芎嗪药物动力学特征的影 响 中国中西医结合杂志,1997,17(1):45-47
[16]毛炯,伍怡和,司徒净普,等.脾阴虚证与血浆胃动素水平关系初探中国中西医结合杂志,1994,14(11):653-655
[17]曾锦章,张万岱,周殿元.慢性胃炎中医辨证与血浆P物质及胃动素浓度的关系.新消化病学杂志,1997,5(6):381-382
[18]易崇勤,叶百宽,金敬善,等.四君子汤对脾虚大鼠胃粘膜细胞酶组织化学及血浆胃肠激素的影响[J].北京中医药大学学报,1997,20(6):31-34
[19]张万岱,姚永莉,宋于刚.脾虚证大鼠组织中胃泌素及生长抑素含量的变化及意义 中国中西医结合脾胃杂志,1998,6(4):223
[20]高云芳,王慧平,王亚洲.四君子汤对脾虚证小鼠血象及胃酸分泌的影响 西北大学学报(自然科学版),2003,33(2):213
[21]徐珊,杨青,成信法.乐胃饮对脾虚实验大鼠胃泌素和胃动素的影响 中国医药学报,2004,19(1):20-22
[22]刘明.益脾汤对脾虚模型大鼠血浆中胃肠激素的影响 福建中医药,2002,33(1):44-45
[23]任平,黄熙,张莉.四君子汤对实验性脾虚大鼠胃排空率的影响 中国中西医结合杂志,2000,20(8):596
[2]陈灏珠等.实用内科学第12版.北京,人民卫生出版社 2005
[3]陈奇.中药药理研究方法学[M].第1版.北京,人民卫生出版社,1994
[4]孙学惠,叶家齐.陈皮及其复方制剂对大鼠胃液分泌的影响.见:陈奇主编.中药药理研究方法学[M].第1版.北京,人民卫生出版社,1994.459-461
[5]陈小铭,倪立,李金恒.宝贝开胃颗粒的药效学研究.医学研究生学报,2006,19(6):535
[6]Mira JP,Cariou A,Grail F,et al.Association of TNF2,a TNFalpha promoter polymorphism,with septic shock susceptibility and mortality:a multi center study[J].JMMA,1998,28(6):561-568.
[7]刘学松.利血平致动物脾虚模型机制的研究.中国医药学报,1989,4(5):68
[8]宗亚峰,陈伟华,皱思湘,等.β-酪啡肽7促大鼠胃内胃泌素的表达.动物学报,2006,52(6):1075-1081
[9]易受乡,阳仁达,严洁,等.针刺对胃黏膜损伤家兔表皮生长因子、生长抑素及生长抑素受体基因表达的影响.世界华人消化杂志,2004,12(7):1721-1723
[10]王建华,刘干中.脾虚的动物模型和补脾药药理研究方法.见:陈奇主编.中药药理研究方法学[M].第1版.北京:人民卫生出版社,1994.1021-1022.
[11]金敬善,王广才,张绳组,等.血清中胃泌素水平与脾虚证的关系.中西医结合杂志,1982,2(1)25-26
[12]聂丹丽.慢性胃病脾虚患者血清胃泌素与胃排空的关系[J].浙江中医杂志,1994,1(29):34)
[13]牟德峻.消化性溃疡中医辨证分型与胃酸、胃泌素、环核苷酸关系的探讨[J].中西医结合杂志,1988,8(9):531
[14]熊洪翔.中医辨证分型对慢性萎缩性胃炎患者空腹血清胃泌素变化的观察[J].福建中医,1983,14(2):50
[15]涂福音.慢性萎缩性胃炎103例中医辨证分型与胃泌素、尿胃蛋白酶关系探讨[J].福建中医药,1990,21(2):11
[16]张万岱.脾虚证患者胃液及胃窦十二指肠黏膜胃泌素含量的研究[J].新消化病学杂志,1993,1(特1):14
[17]张桂珍.脾虚证患者胃窦G细胞体视学定量分析[J].中西医结合杂志,1989,9(12):711
[18]智发朝,张万岱,宋于刚.生长抑素与脾虚证关系的研究.新消化病学杂志,1993;1(特1)::49-50
[19]马建伟,徐丽梅,郝刚,等.脾虚证与血浆和胃窦组织中生长抑素关系的探讨.实用中西医结合杂志,1995,8(11):644-645
[20]宋于刚,姚永莉,张万岱.脾虚证与体液胃泌素及生长抑素关系的实验研究.胃肠病学,1998,3(3):182-183
[21]张万岱,姚永莉,宋于刚.脾虚证大鼠组织中胃泌素及生长抑素含量的变化及意义 中国中西医结合脾胃杂志,1998,6(4):2231
[22]任平,夏天,张忠兵,等.脾虚泄泻与血浆及肠组织中胃动素的关系 第四军医大学学报,1991,12(5):391-392
[23]任平,宋国增,夏天,等.对脾虚泄泻患者胃动素及前列腺素的初探 中国医药学报,1993;8(1):22-24
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