大鼠C6脑胶质瘤3.0T MR-DTI及瘤周水肿浸润组织FA值与AQP1的相关性研究
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摘要
第一部分Wistar大鼠C6脑胶质瘤模型的建立
目的：建立稳定可靠的Wistar大鼠C6脑胶质瘤晚期阶段模型。
材料和方法：1、C6细胞在含10%FBS、1%青链霉素混合液的RPMI-1640完全培养基中,置于37℃、5%CO2培养箱中培养,胰酶消化、终止,采集对数生长期的C6细胞计数,检测细胞活性,加完全培养基调整浓度。2、采用随机数字表将22只Wistar大鼠分成实验组17只,假手术组(对照组)5只。通过立体定向技术,将浓度大于1.0x106.10μ1的C6细胞接种至实验组大鼠右侧尾状核区,假手术组大鼠在相同部位注射完全培养基。3、肿瘤细胞接种后约3-4周对相应大鼠进行磁共振扫描和病理观察。
结果：1、17只大鼠接种肿瘤细胞,1只死于术后约1h,1只于接种后15日不明原因死亡(随后病理证实有肿瘤形成),其余15只肿瘤细胞接种大鼠在随后的MRI和病理检查中均证实有肿瘤形成,接种成功率100%。假手术组5只大鼠均存活,且未见肿瘤形成。2、术后经磁共振及实验结束后的病理证实肿瘤细胞接种后约3-4周存在瘤周水肿和/或肿瘤细胞浸润。
结论：Wistar大鼠C6脑胶质瘤模型稳定可靠,生长迅速,接种成功率高,肿瘤细胞接种后约3-4周,可满足瘤周水肿和/或肿瘤浸润实验要求。
第二部分大鼠C6脑胶质瘤在体3.0T磁共振张量成像研究
目的：探讨3.0T磁共振张量成像在Wistar大鼠C6胶质瘤肿瘤区和对侧正常脑实质区的各向异性分数(FA)值及平均弥散系数(MD)值,并比较两种不同的方法选取感兴趣区(ROI)对测量结果的影响。
材料和方法：采用随机数字表将22只Wistar大鼠分成实验组17只,假手术组(对照组)5只。通过立体定向技术,将浓度大于1.0x106/10μ1的C6细胞接种至实验组大鼠右侧尾状核区,假手术组大鼠在相同部位注射完全培养基。肿瘤细胞接种后约3-4周,利用3.0T高场强医用磁共振扫描仪,大鼠专用线圈对大鼠C6胶质瘤模型行常规MRI、DT工、T1WI及SAG T1-3D-bravo增强检查,结合T2WI、FLAIR、T1WI及SAGT1-3D-bravo增强确定肿瘤与水肿区,借助Function Tool软件对DTI数据后处理,避开出血、坏死区,选取多个即3个等大(2mmm2,9pix)ROI和单个较大ROI,获得肿瘤实质和对侧正常脑实质区的FA及MD值。
结果：15只成瘤大鼠中,肿瘤区和对侧正常脑实质区3个等大ROI FA平均值分别为(0.1075±0.02)和(0.312±0.024),而3个等大ROIMD值分别为(0.944±0.029)×10-3mm2/s和(0.808±0.036)×10-3mm2/s,两组结果经两样本t检验显示差别均有统计学意义(P＜0.05)。肿瘤区和对侧正常脑实质区单个较大ROI FA平均值分为(0.118±0.039)和(0.310±0.052),而单个较大ROI MD平均值分别为(0.941±0.047)×10-3mm2/s和(0.806±0.043)×10-3mm2/s,两组结果经两样本t检验显示差别亦有统计学意义(P＜0.05)。两种不同的方法选取肿瘤区ROI获得的FA值经配对t检验显示有统计学意义(P<0.05),而MD值间无统计学意义(P＞0.05)。
结论：用临床3.0T MRI行大鼠C6胶质瘤DTI检查切实可行,通过测量肿瘤区、对侧正常脑实质区FA及MD值,为以后的科研工作提供了有用的参考。方法学上,本研究发现两种不同的方法选取ROI对FA值测量结果有影响,而对MD值测量结果无明显影响。
第三部分AQP1在鼠脑C6胶质瘤瘤周水肿浸润组织中的表达与FA值的相关性研究
目的：研究Wistar大鼠C6脑胶质瘤瘤周水肿浸润组织的磁共振弥散张量成像(MR-DTI)的特征,并与免疫组织化学水通道蛋白1(AQP1)对照,探讨AQP1在胶质瘤瘤周水肿浸润组织中的表达与MR-DTI参数部分各向异性分数(FA)的相关性。
材料与方法：采用随机数字表将22只Wistar大鼠分成实验组17只,假手术组(对照组)5只。通过立体定向技术,将浓度大于1.0×106/10ul的C6细胞接种至实验组大鼠右侧尾状核区,假手术组大鼠在相同部位注射完全培养基。肿瘤细胞接种后约3～4周,利用3.0T高场强医用磁共振扫描仪,大鼠专用线圈对大鼠C6胶质瘤模型行常规MRI、DTI、FLAIR、T1WI及SAG T1-3D-bravo增强检查,结合T2WI、FLAIR.T1WI及SAG T1-3D-bravo增强确定肿瘤与水肿区,借助Function Tool软件对DTI数据后处理,避开出血、坏死等影响结果的区域,选取多个即3个等大(2mm2,9pix)ROI,获得相应部位的FA值。设肿瘤对侧正常脑组织为内对照。MRI扫描完成后立即行4%的多聚甲醛灌注固定全脑,之后过量1%的戊巴比妥钠处死大鼠取全脑固定,选取与DTI肿瘤最大层面相对应的瘤周水肿区脑组织做连续冠状切片,进行HE染色和AQP1抗体免疫组织化学检查。Pearson相关分析法检测FA值和相应部位的AQPl阳性表达的IOD值的相关性,计算相关系数,并进行t检验,以P<0.05为有统计学意义。
结果：15只成瘤大鼠中,瘤周水肿区约3-4周3个等大ROI FA平均值为(0.204±0.036),较对侧正常脑组织多个ROI FA平均值(0.310±0.027)降低,不过没有瘤体降低明显,两组结果经两样本t检验显示差别有统计学意义(P＜0.05)。Pearson相关分析显示,肿瘤水肿浸润组织的FA值与AQP1阳性表达IOD值之间存在负相关(r=-0.81,P<0.05)。
结论：Wistar大鼠C6脑胶质瘤瘤周水肿浸润组织MR-DTI参数FA值与AQP1之间存在负相关,随着AQP1阳性表达程度的增高,FA值降低。
Part I:The establishment of Wistar rat C6 brain glioma model
Objective:To establish the stable and reliable late stage Wistar rat C6 brain glioma model.
Materials and Methods:1.Rat C6 glioma cell lines were propagated in RPMI-1640 medium,supplemented with 10% fetal bovine serum,1% Penicillin-Streptomycin Solution,and were cultured with 5%C02 at 37℃in incubator. The tumor cells were maintained in continuous monolayer cell cultures in coming tissue culture flasks.Tumor cells were collected during logarithmic phase to count and detect cell activity.The concentrati-on were adjusted with complete medium.2.22 Wistar rats were divided into experimental group of 17 and control group of 5 by the random number table.The concentration of more than 1.0×106/10ul glioma cells and complete medium were injected stereotactically into the right caudate nucleus of the experimental group and control group, respectively.3. MRI scans and pathological observations were performed about 3～4 weeks after implantation for the rats.
Results:1. One of the experimental group died after about lh,and another died of unknown causes 15 days afterr implantation (the pathology demonstrated tumor formation), the other 15 rats were confirmed the tumor formation by the subsequent MRI and pathological 硕士学位论文英文摘要examination.The control group of 5 were alive, and no tumor formation. 2. There is peritumor edema and/or tumor cell invasion about 3～4 weeks after implantation by Postoperative MRI and pathological examination.
Conclusion:Wistar rat C6 glioma model is a stable and reliable model, After implantation,tumor grows rapidly,and the success rate is very high.The model can meet experimental requirements for the peritumoral edema and/or tumor infiltration about 3 to 4 weeks after inoculation.
PartⅡ:The study of diffusion tensor imaging using 3.0T MR scanner in rat C6 brain glioma model in vivo
Objective:To study the average fractional anisotropy(FA) and mean diffusivity (MD) values in the wistar rat C6 brain glioma regions and the contralateral normal brain parenchyma using 3.0T Magnetic resonance scanner,and compare the two different selective methods of region of interest (ROI).
Materials and Methods:22 Wistar rats were divided into experim-ental group of 17 and control group of 5 by the random number table. The concentration of more than 1.0×106/10ul glioma cells and complete medium were stereotactically injected into the right caudate nucleus of the experimental group and controlgroup,respectively.Conventional MRI、DTI、enhanced T1WI and SAG T1-3D-bravo scans were performed using the GE Signa HDx 3.0T MRI scanner with the rat special coil about 3～4 weeks after implantation.And glioma and peritumoral edema were distin-guished by T2WI、FLAIR、enhanced T1WI and SAG T1-3D-bravo scans.Postproeessing was done by the DTI specific softw-are Function Tool to gain FA and MD images. ROIs were drawn,avoiding hemorrhage and necrosis areas, in different selective methods (3 equal-sized ROIs and a single larger ROI) in tumor parenchyma and the contralateral normal brain parenchyma.
Results:The average FA values of 3 equal-sized ROIs in the 15 wistar rats C6 brain glioma regions and the contralateral normal brain parenchyma were (0.1075±0.02) and (0.312±0.024), respectively;the average MD values were (0.944±0.029)×10-3mm2/s and (0.808±0.036)×10-3mm2/s, respectively.Two independent-sample T test of the two groups showed statistically significant difference (P<0.05).The average FA values of single larger ROI in the wistar rat C6 brain glioma regions and the contralateral normal brain parenchyma were (0.118±0.039) and (0.310±0.052),respectively; the average MD values were(0.941±0.047)×10-3 mm2/s and (0.806±0.043)×10-3 mm2/s,respectively.Two independ-ent-sample T test of the two groups also showed statistically significant difference (P<0.05). Paired-sample T test of obtained FA values by the two different measurement methods showed statistically significant difference(P<0.05),while no statistical significance between the MD values (P> 0.05).
Conclusion:It is feasible to perform DTI scan in the rat C6 brain glioma model using a clinical 3.0T MR scanner,and the FA and MD values measured in the rat C6 brain glioma regions and the contralateral normal brain parenchyma can be used for reference in future studies. Methodologically, the study showed the two different selective methods of region of interest could influence the measurements of FA vales, while have no effect on the measurements of MD values.
Part III:The correlative study of AQP1 expression in rat brain glioma infiltrated peritumoral edema tissue and the FA value
Objective:Magnetic resonance diffusion tensor imaging (MR-DTI) was performed in Wistar rat brain glioma infiltrated peritumoral edema tissue to analyze its features, and explore the correlation of AQP1 expression in rat brain glioma infiltrated peritumoral edema tissue by immunohistochemical findings and the FA value.
Materials and Methods:22 Wistar rats were divided into experimental group of 17 and control group of 5 by the random number table.The concentration of more than 1.0×106/10ul glioma cells and complete medium were injected stereotactically into the right caudate nucleus of the experimental group and control group,respectively. Conv-entional MRI, DTI, enhanced T1WI and SAG T1-3D-bravo scans were performed using the GE Signa HDx 3.0T MRI scanner with the rat special coil about 3-4 weeks after implantation. And gliomas and peritumoral edema were distinguished by T2WI、FLAIR、enhanced T1WI and SAG T1-3D-bravo scans.Postproeessing was done using the DTI specific software Function Tool to gain FA images. ROIs were drawn, av-oiding hemorrhage and necrosis areas, in 3 equal-sized (2mm2,9pix). ROIs in peritumoral edema and the contralateral normal brain parenchy-ma to gain FA values. Let the contralateral normal brain tissue as an internal control. The whole rat brain was fixed by 4% paraformaldehyde perfusion after MRI scans immediately, and then the rats were sacrificed to take the whole brain fixed by excess of 1% sodium pentobarbital. Peritumoral brain edema tissue corresponding the maximum level of the tumor were selected to do continuous coronal sections. Each rat brain was examined histologically using HE and immunohistochemical staining for AQP1. Pearson correlation analysis was used to determine the relations between FA values and the IOD value of AQP1 expression.Correlation coefficient was also calculated and T test was made with statistical significance when P<0.05.
Results:The average FA values of 3 equal-sized ROIs in the 15 rats C6 brain glioma peritumoral edema tissue about 3～4 weeks after implantation were (0.204±0.036). The results were lower than the average FA values (0.310±0.027) of the contralateral normal brain tissue. Two independent-sample T test of two groups showed statistically significant difference (P<0.05).Correlation were Negative between FA values of infiltrated peritumoral edema tissue and the IOD value of AQP1 expression(r=-0.81,P<0.05).
Conclusion:The correlation of AQP1 expression in rat brain glioma infiltrated peritumoral edema tissue and the FA value were negative.with the increase of AQP1 expression level, FA value decreased.
目的：建立稳定可靠的Wistar大鼠C6脑胶质瘤晚期阶段模型。
材料和方法：1、C6细胞在含10%FBS、1%青链霉素混合液的RPMI-1640完全培养基中,置于37℃、5%CO2培养箱中培养,胰酶消化、终止,采集对数生长期的C6细胞计数,检测细胞活性,加完全培养基调整浓度。2、采用随机数字表将22只Wistar大鼠分成实验组17只,假手术组(对照组)5只。通过立体定向技术,将浓度大于1.0x106.10μ1的C6细胞接种至实验组大鼠右侧尾状核区,假手术组大鼠在相同部位注射完全培养基。3、肿瘤细胞接种后约3-4周对相应大鼠进行磁共振扫描和病理观察。
结果：1、17只大鼠接种肿瘤细胞,1只死于术后约1h,1只于接种后15日不明原因死亡(随后病理证实有肿瘤形成),其余15只肿瘤细胞接种大鼠在随后的MRI和病理检查中均证实有肿瘤形成,接种成功率100%。假手术组5只大鼠均存活,且未见肿瘤形成。2、术后经磁共振及实验结束后的病理证实肿瘤细胞接种后约3-4周存在瘤周水肿和/或肿瘤细胞浸润。
结论：Wistar大鼠C6脑胶质瘤模型稳定可靠,生长迅速,接种成功率高,肿瘤细胞接种后约3-4周,可满足瘤周水肿和/或肿瘤浸润实验要求。
第二部分大鼠C6脑胶质瘤在体3.0T磁共振张量成像研究
目的：探讨3.0T磁共振张量成像在Wistar大鼠C6胶质瘤肿瘤区和对侧正常脑实质区的各向异性分数(FA)值及平均弥散系数(MD)值,并比较两种不同的方法选取感兴趣区(ROI)对测量结果的影响。
材料和方法：采用随机数字表将22只Wistar大鼠分成实验组17只,假手术组(对照组)5只。通过立体定向技术,将浓度大于1.0x106/10μ1的C6细胞接种至实验组大鼠右侧尾状核区,假手术组大鼠在相同部位注射完全培养基。肿瘤细胞接种后约3-4周,利用3.0T高场强医用磁共振扫描仪,大鼠专用线圈对大鼠C6胶质瘤模型行常规MRI、DT工、T1WI及SAG T1-3D-bravo增强检查,结合T2WI、FLAIR、T1WI及SAGT1-3D-bravo增强确定肿瘤与水肿区,借助Function Tool软件对DTI数据后处理,避开出血、坏死区,选取多个即3个等大(2mmm2,9pix)ROI和单个较大ROI,获得肿瘤实质和对侧正常脑实质区的FA及MD值。
结果：15只成瘤大鼠中,肿瘤区和对侧正常脑实质区3个等大ROI FA平均值分别为(0.1075±0.02)和(0.312±0.024),而3个等大ROIMD值分别为(0.944±0.029)×10-3mm2/s和(0.808±0.036)×10-3mm2/s,两组结果经两样本t检验显示差别均有统计学意义(P＜0.05)。肿瘤区和对侧正常脑实质区单个较大ROI FA平均值分为(0.118±0.039)和(0.310±0.052),而单个较大ROI MD平均值分别为(0.941±0.047)×10-3mm2/s和(0.806±0.043)×10-3mm2/s,两组结果经两样本t检验显示差别亦有统计学意义(P＜0.05)。两种不同的方法选取肿瘤区ROI获得的FA值经配对t检验显示有统计学意义(P<0.05),而MD值间无统计学意义(P＞0.05)。
结论：用临床3.0T MRI行大鼠C6胶质瘤DTI检查切实可行,通过测量肿瘤区、对侧正常脑实质区FA及MD值,为以后的科研工作提供了有用的参考。方法学上,本研究发现两种不同的方法选取ROI对FA值测量结果有影响,而对MD值测量结果无明显影响。
第三部分AQP1在鼠脑C6胶质瘤瘤周水肿浸润组织中的表达与FA值的相关性研究
目的：研究Wistar大鼠C6脑胶质瘤瘤周水肿浸润组织的磁共振弥散张量成像(MR-DTI)的特征,并与免疫组织化学水通道蛋白1(AQP1)对照,探讨AQP1在胶质瘤瘤周水肿浸润组织中的表达与MR-DTI参数部分各向异性分数(FA)的相关性。
材料与方法：采用随机数字表将22只Wistar大鼠分成实验组17只,假手术组(对照组)5只。通过立体定向技术,将浓度大于1.0×106/10ul的C6细胞接种至实验组大鼠右侧尾状核区,假手术组大鼠在相同部位注射完全培养基。肿瘤细胞接种后约3～4周,利用3.0T高场强医用磁共振扫描仪,大鼠专用线圈对大鼠C6胶质瘤模型行常规MRI、DTI、FLAIR、T1WI及SAG T1-3D-bravo增强检查,结合T2WI、FLAIR.T1WI及SAG T1-3D-bravo增强确定肿瘤与水肿区,借助Function Tool软件对DTI数据后处理,避开出血、坏死等影响结果的区域,选取多个即3个等大(2mm2,9pix)ROI,获得相应部位的FA值。设肿瘤对侧正常脑组织为内对照。MRI扫描完成后立即行4%的多聚甲醛灌注固定全脑,之后过量1%的戊巴比妥钠处死大鼠取全脑固定,选取与DTI肿瘤最大层面相对应的瘤周水肿区脑组织做连续冠状切片,进行HE染色和AQP1抗体免疫组织化学检查。Pearson相关分析法检测FA值和相应部位的AQPl阳性表达的IOD值的相关性,计算相关系数,并进行t检验,以P<0.05为有统计学意义。
结果：15只成瘤大鼠中,瘤周水肿区约3-4周3个等大ROI FA平均值为(0.204±0.036),较对侧正常脑组织多个ROI FA平均值(0.310±0.027)降低,不过没有瘤体降低明显,两组结果经两样本t检验显示差别有统计学意义(P＜0.05)。Pearson相关分析显示,肿瘤水肿浸润组织的FA值与AQP1阳性表达IOD值之间存在负相关(r=-0.81,P<0.05)。
结论：Wistar大鼠C6脑胶质瘤瘤周水肿浸润组织MR-DTI参数FA值与AQP1之间存在负相关,随着AQP1阳性表达程度的增高,FA值降低。
Part I:The establishment of Wistar rat C6 brain glioma model
Objective:To establish the stable and reliable late stage Wistar rat C6 brain glioma model.
Materials and Methods:1.Rat C6 glioma cell lines were propagated in RPMI-1640 medium,supplemented with 10% fetal bovine serum,1% Penicillin-Streptomycin Solution,and were cultured with 5%C02 at 37℃in incubator. The tumor cells were maintained in continuous monolayer cell cultures in coming tissue culture flasks.Tumor cells were collected during logarithmic phase to count and detect cell activity.The concentrati-on were adjusted with complete medium.2.22 Wistar rats were divided into experimental group of 17 and control group of 5 by the random number table.The concentration of more than 1.0×106/10ul glioma cells and complete medium were injected stereotactically into the right caudate nucleus of the experimental group and control group, respectively.3. MRI scans and pathological observations were performed about 3～4 weeks after implantation for the rats.
Results:1. One of the experimental group died after about lh,and another died of unknown causes 15 days afterr implantation (the pathology demonstrated tumor formation), the other 15 rats were confirmed the tumor formation by the subsequent MRI and pathological 硕士学位论文英文摘要examination.The control group of 5 were alive, and no tumor formation. 2. There is peritumor edema and/or tumor cell invasion about 3～4 weeks after implantation by Postoperative MRI and pathological examination.
Conclusion:Wistar rat C6 glioma model is a stable and reliable model, After implantation,tumor grows rapidly,and the success rate is very high.The model can meet experimental requirements for the peritumoral edema and/or tumor infiltration about 3 to 4 weeks after inoculation.
PartⅡ:The study of diffusion tensor imaging using 3.0T MR scanner in rat C6 brain glioma model in vivo
Objective:To study the average fractional anisotropy(FA) and mean diffusivity (MD) values in the wistar rat C6 brain glioma regions and the contralateral normal brain parenchyma using 3.0T Magnetic resonance scanner,and compare the two different selective methods of region of interest (ROI).
Materials and Methods:22 Wistar rats were divided into experim-ental group of 17 and control group of 5 by the random number table. The concentration of more than 1.0×106/10ul glioma cells and complete medium were stereotactically injected into the right caudate nucleus of the experimental group and controlgroup,respectively.Conventional MRI、DTI、enhanced T1WI and SAG T1-3D-bravo scans were performed using the GE Signa HDx 3.0T MRI scanner with the rat special coil about 3～4 weeks after implantation.And glioma and peritumoral edema were distin-guished by T2WI、FLAIR、enhanced T1WI and SAG T1-3D-bravo scans.Postproeessing was done by the DTI specific softw-are Function Tool to gain FA and MD images. ROIs were drawn,avoiding hemorrhage and necrosis areas, in different selective methods (3 equal-sized ROIs and a single larger ROI) in tumor parenchyma and the contralateral normal brain parenchyma.
Results:The average FA values of 3 equal-sized ROIs in the 15 wistar rats C6 brain glioma regions and the contralateral normal brain parenchyma were (0.1075±0.02) and (0.312±0.024), respectively;the average MD values were (0.944±0.029)×10-3mm2/s and (0.808±0.036)×10-3mm2/s, respectively.Two independent-sample T test of the two groups showed statistically significant difference (P<0.05).The average FA values of single larger ROI in the wistar rat C6 brain glioma regions and the contralateral normal brain parenchyma were (0.118±0.039) and (0.310±0.052),respectively; the average MD values were(0.941±0.047)×10-3 mm2/s and (0.806±0.043)×10-3 mm2/s,respectively.Two independ-ent-sample T test of the two groups also showed statistically significant difference (P<0.05). Paired-sample T test of obtained FA values by the two different measurement methods showed statistically significant difference(P<0.05),while no statistical significance between the MD values (P> 0.05).
Conclusion:It is feasible to perform DTI scan in the rat C6 brain glioma model using a clinical 3.0T MR scanner,and the FA and MD values measured in the rat C6 brain glioma regions and the contralateral normal brain parenchyma can be used for reference in future studies. Methodologically, the study showed the two different selective methods of region of interest could influence the measurements of FA vales, while have no effect on the measurements of MD values.
Part III:The correlative study of AQP1 expression in rat brain glioma infiltrated peritumoral edema tissue and the FA value
Objective:Magnetic resonance diffusion tensor imaging (MR-DTI) was performed in Wistar rat brain glioma infiltrated peritumoral edema tissue to analyze its features, and explore the correlation of AQP1 expression in rat brain glioma infiltrated peritumoral edema tissue by immunohistochemical findings and the FA value.
Materials and Methods:22 Wistar rats were divided into experimental group of 17 and control group of 5 by the random number table.The concentration of more than 1.0×106/10ul glioma cells and complete medium were injected stereotactically into the right caudate nucleus of the experimental group and control group,respectively. Conv-entional MRI, DTI, enhanced T1WI and SAG T1-3D-bravo scans were performed using the GE Signa HDx 3.0T MRI scanner with the rat special coil about 3-4 weeks after implantation. And gliomas and peritumoral edema were distinguished by T2WI、FLAIR、enhanced T1WI and SAG T1-3D-bravo scans.Postproeessing was done using the DTI specific software Function Tool to gain FA images. ROIs were drawn, av-oiding hemorrhage and necrosis areas, in 3 equal-sized (2mm2,9pix). ROIs in peritumoral edema and the contralateral normal brain parenchy-ma to gain FA values. Let the contralateral normal brain tissue as an internal control. The whole rat brain was fixed by 4% paraformaldehyde perfusion after MRI scans immediately, and then the rats were sacrificed to take the whole brain fixed by excess of 1% sodium pentobarbital. Peritumoral brain edema tissue corresponding the maximum level of the tumor were selected to do continuous coronal sections. Each rat brain was examined histologically using HE and immunohistochemical staining for AQP1. Pearson correlation analysis was used to determine the relations between FA values and the IOD value of AQP1 expression.Correlation coefficient was also calculated and T test was made with statistical significance when P<0.05.
Results:The average FA values of 3 equal-sized ROIs in the 15 rats C6 brain glioma peritumoral edema tissue about 3～4 weeks after implantation were (0.204±0.036). The results were lower than the average FA values (0.310±0.027) of the contralateral normal brain tissue. Two independent-sample T test of two groups showed statistically significant difference (P<0.05).Correlation were Negative between FA values of infiltrated peritumoral edema tissue and the IOD value of AQP1 expression(r=-0.81,P<0.05).
Conclusion:The correlation of AQP1 expression in rat brain glioma infiltrated peritumoral edema tissue and the FA value were negative.with the increase of AQP1 expression level, FA value decreased.
引文
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