西南地区小果油茶群体遗传结构及脂肪酸成分变异分析
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摘要
小果油茶(Camellia meiocarpa Hu.)是一种重要的木本油料树种,在我国长江以南地区广泛分布。本研究对西南地区小果油茶群体资源从脂肪酸组成与分子标记方面进行了系统评价,其主要结果如下：
(1)利用SRAP标记对6个小果油茶群体的180份材料进行遗传多样性研究。从87对SRAP引物中共筛选出9对多态性理想的引物组合,共扩增出184条带,其中多态性条带184条,多态性位点比率高达100%。平均有效等位基因数为1.7603,180份小果油茶总Nei基因多样性指数为0.4279,平均Shannon信息指数为0.6183,表明小果油茶群体具有较丰富的遗传多样性,遗传变异主要分布在群体内,群体间变异相对较小
(2)从小果油茶茶油脂肪酸成分的多重比较得出,群体间油茶的含油率变异较大,脂肪酸成分变异不显著。且脂肪酸成分与地区的地理、环境因子相关性不显著,其遗传性状是主要的影响因子。
(3)运用分子标记与脂肪酸组成变异分析,结果显示二种方法揭示的小果油茶群体聚类结果较一致,表明这二种方法在评价小果油茶遗传多样性中的耦合性较好。
本研究还通过小果油茶遗传多样性和遗传结构的分析提出了对该物种遗传多样性的保护策略。
Camellia meiocarpa Hu is a is one of the important oil tree species in south China. In this study, population resources of Camellia meiocarpa Hu were systematically evaluated at fatty acid compositions and molecular marker.The main results are as following:
1. The genetic diversity of Camellia meiocarpa Hu was analyzed by SRAP markers.9 primers selected from 87 candidates were applied to the amplification which produced 184 bands. Of them 184 bands (100%) were polymorphic. The average effective number of alleles. Nei's gene diversity and Shannon's information index were 1.7603, the genetic diversity of 180 Camellia meiocarpa Hu materials was high with Nei's gene diversity of 0.4279 and Shannon's information index of 0.6183,the mainly genetic variation of Camellia meiocarpa Hu existed in the populations.
2. By Multi-comparison to the composition of tea oil-Fatty acid in Camellia meiocarpa Hu, the result that the variation of value of oilness was greater among tea oil while the variation of Fatty acid was limited was brought. The composition of fatty acid was not remarkable relevance with the Geographic and environmental Factors. The main impact factor was its Genetic Character.
3. The result showed that the Groups clustering results of Camellia meiocarpa Hu were Inclined by means of MMs and analyzing the variation of Fatty acid. It showed that the Coupling of this two kinds of methods were better in evaluating the Genetic Diversity of Camellia meiocarpa Hu.
Based on the genetic information available for the native Camellia meiocarpa Hu, some conservation strategies were proposed.
(1)利用SRAP标记对6个小果油茶群体的180份材料进行遗传多样性研究。从87对SRAP引物中共筛选出9对多态性理想的引物组合,共扩增出184条带,其中多态性条带184条,多态性位点比率高达100%。平均有效等位基因数为1.7603,180份小果油茶总Nei基因多样性指数为0.4279,平均Shannon信息指数为0.6183,表明小果油茶群体具有较丰富的遗传多样性,遗传变异主要分布在群体内,群体间变异相对较小
(2)从小果油茶茶油脂肪酸成分的多重比较得出,群体间油茶的含油率变异较大,脂肪酸成分变异不显著。且脂肪酸成分与地区的地理、环境因子相关性不显著,其遗传性状是主要的影响因子。
(3)运用分子标记与脂肪酸组成变异分析,结果显示二种方法揭示的小果油茶群体聚类结果较一致,表明这二种方法在评价小果油茶遗传多样性中的耦合性较好。
本研究还通过小果油茶遗传多样性和遗传结构的分析提出了对该物种遗传多样性的保护策略。
Camellia meiocarpa Hu is a is one of the important oil tree species in south China. In this study, population resources of Camellia meiocarpa Hu were systematically evaluated at fatty acid compositions and molecular marker.The main results are as following:
1. The genetic diversity of Camellia meiocarpa Hu was analyzed by SRAP markers.9 primers selected from 87 candidates were applied to the amplification which produced 184 bands. Of them 184 bands (100%) were polymorphic. The average effective number of alleles. Nei's gene diversity and Shannon's information index were 1.7603, the genetic diversity of 180 Camellia meiocarpa Hu materials was high with Nei's gene diversity of 0.4279 and Shannon's information index of 0.6183,the mainly genetic variation of Camellia meiocarpa Hu existed in the populations.
2. By Multi-comparison to the composition of tea oil-Fatty acid in Camellia meiocarpa Hu, the result that the variation of value of oilness was greater among tea oil while the variation of Fatty acid was limited was brought. The composition of fatty acid was not remarkable relevance with the Geographic and environmental Factors. The main impact factor was its Genetic Character.
3. The result showed that the Groups clustering results of Camellia meiocarpa Hu were Inclined by means of MMs and analyzing the variation of Fatty acid. It showed that the Coupling of this two kinds of methods were better in evaluating the Genetic Diversity of Camellia meiocarpa Hu.
Based on the genetic information available for the native Camellia meiocarpa Hu, some conservation strategies were proposed.
引文
[1]庄瑞林.中国油茶(第2版)[M].北京：中国林业出版社,2008
[2]胡芳名,谭晓风,刘惠民.中国主要经济树种栽培与利用[M].北京：中国林业出版社,2006.
[3]欧阳嘉,曹叶青,樊可可,沈树宝.多种油脂的脂肪酸组成分析[J].南京化工大学学报(自然科学版),1997,(02).
[4]丛玲美,姚小华,费学谦,王开良,王亚萍,王年金.长期贮藏对茶油酸值和过氧化值的影响[J].林业科学研究,2007,(02)
[5]李纪元.种好旱地,粮油丰收[J].山西农业科学,1981(06)
[6]姚小华.油茶高效实用栽培技术[M].北京：科学出版社,2010.
[7]庄瑞林,王德斌,蔡肖群,匡元玉,熊年康.山茶大树嫁接的研究[J].经济林研究1994,12(2)
[8]周盛,朱金惠,肖景治等.油茶远缘杂交育种试验[J].经济林研究,2001,19(1)：20-25
[9]傅瑞树等.油茶某些生理活动的昼夜变化[J].林业科技通讯,1984,(8)：11-14
[10]李建安,张日清,石明旺,杨伟.油茶两物种花药培养愈伤组织诱导试验[J].经济林研究2003,21(3)：36-38
[11]闻丽,张日清,李建安,吕芳德.油荼种质改良现状及其花药培养技术的应用前景[J].经济林研究2004,22(4)：87-90
[12]张日清,闻丽,刘友全,李建安,漆龙霖,栗彬,张春生.低温预处理对油茶花药愈伤组织诱导的影响[J].中南林学院学报,2005(06)
[13]丁檀磊,张日清,刘友全,闻丽.油茶12个物种花药愈伤组织诱导及继代培养[J].经济林研究,2007,25(1)：20-24
[14]沈德绪,王元裕,陈力耕.柑橘遗传育种学[M].北京,科学出版社,1998
[15]杨学明,裔传灯.植物染色体原位杂交技术及其在稻属研究中的应用[J].植物遗传资源学报[J],.2004(04)
[16]张玉静.分子遗传学[M].北京：科学出版社,2002,9-46
[17]刘军,陈益泰,姜景民,何贵平,余国民.毛红椿群体遗传结构的SSR分析[J].林业科学研究,2009,22(1)：37-41
[18]Godwir I D, Aitke R E, Smith L W. Application of inter-simply sequence repeat (ISSR) markers to plant genetics [J]. Electrophoresis,1997,18:1524-1528
[19]Qian W, Hong D Y, Ge S. Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers [J]. Theor Appl Genet,2001,102:440-449
[20]刘平等.赞皇大枣的群体遗传多样性评价[J].果树学报,2006,23(5)：685-689
[21]Kantety R V, Rotal M L, Matthews D E, Sorrells M E. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat [J]. Plant Mol Biol,2002,48:501-510
[22]Femamdez M E, Figueiras A M, Benito C. The use of ISSR and RAPD markets for detecting DNA polymorphism, gene type identification and diversity among barley cultivars with known origin [J]. Theor Appl Genet,2002,104:845-851
[23]李斌,顾万春.白皮松保育遗传学——天然群体遗传多样性评价与保护策略[J].林业科学,2005,41(1)：57-64
[24]高秀琴,兰进好,林琪,等.部分旱地小麦品种(系)遗传多样性的SSR分析[J].麦类作物学报,2008,28(4)：577-581
[25]严学兵,王堑,周禾,等.不同来源SSR标记在我国披碱草属植物的通用性和效率评价[J].草业学报,2008,17(6)：112-120
[26]Amel S, Mikhtar T, Salwa Z, et al. Inter-simple sequence repeat fingerprint s to assess genetic diversity in trnisian fig (Ficus carica L.) germplasm[J]. Genetic Resources and Crop Evolution,2004,51:269-275.
[27]Hassanien H A, Gilbey J. Genetic diversity and differentiation of Niletilap ia (Oreochrom is niloticus) revealed by DNA microsatellites[J]. Aquaculture Research, 2005,36,(14):1450-1457
[28]EP.NieeseJ.I.HormazaGH.MeGranahan. Molecular characterization and genetic relatedness among walnut (Juglans regia L.) genotypes base on RAPD markers [J]. Euphytica,1998,101 (2):199-206
[29]Echt C S, Deverno L L, Anzidei M. Chlorop last microsatellites reveal population genetic diversity in red pine, Pinus resinosa Ait [J]. Molecular Ecology, 1998,7(3):307-316
[30]LI G, QUIROS C F. Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:It's application to mapping and gene tagging in Brassica [J]. Theoretical and Applied Genetics.2001.103:455-461
[31]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].北京：科学出版社,2002：1-9
[32]WANG Gang.Construction of a Cucumber Genetic Linkage Map with RAPD, SRAP and SSR Markers[J]. Journal of Shanghai Jiaotong University (Science).2005, 45-49.
[33]Ferriol M, PicoB,Nuez F. Genetic diversity of a germplasm collection of cucurbita pepa using SRAP and AFLP markers[J].Theor Appl Genet,2003,107:271-282.
[34]李廷春,樊洪泓,高正良,周应兵,杨华应.丹参遗传多样性的SRAP标记分析[J].核农学报,208(05)
[35]沈程文,黄建安等.利用SRAP和ISSR标记分析广东茶树种质资源的遗传多样性[J].核农学报,2010,24(5)：948-955
[36]李怀志,韩洪强,李维为等.利用SRAP技术评价茄子种质资源遗传多样性[A]：中国园艺学会十届会员代表大会暨学术讨论论文集,2007
[37]刘建丰,王志德,刘艳华,牟建民.应用SRAP标记研究烟草种质资源的遗传多样性[J].中国烟草科学,2007(05)
[38]路娟,张绍铃等.樱桃SRAP-PCR体系优化及其遗传多样性分析[J].果树学报2009,26(2)：163-169
[39]左丹丹,赵海涛等.蜡梅天然群体遗传多样性的SRAP标记分析[J].园艺学报,2009,36(8)：1197-1202
[40]张春雨等.新疆野苹果群体遗传结构和遗传多样性的SRAP分析[J].园艺学报,2009,36(1)：7-14
[41]凌瑶等.西南五省区及非洲野生狗牙根种质基于SRAP标记的遗传多样性分析[J].草业学报,2010,19(2)：196-203
[42]England P R, Usher A V, Whelan R J, et al. Microsatellite diversity and genetic structure of fragmented populations of the rare, fire-dependent shrub Grevillea m acleayana [J]. Molecular Ecology,2002,11 (6):967-977
[43]鄢家俊等.青藏高原东南缘老芒麦自然居群遗传多样性的SRAP和SSR分析[J].草业学报,2010,19(4)：122-134
[44]刘月光,滕永勇,潘辰,韩延闯等.应用SRAP标记对莲藕资源的聚类分析[J].氨基酸和生物资源,2006(01)
[45]于拴仓,柴敏,姜立纲.主要樱桃番茄品种的分子鉴别[J].华北农学报,2005(05)
[46]张建成,王传堂,焦坤,杨新道SRAP标记技术在花生种子纯度鉴定中的应用[J].中国农学通报,2005(12)
[47]林忠旭,张献龙,聂以春.新型标记SRAP在棉花F2分离群体及遗传多样性评价中的适用性分析[J].遗传学报,2004,31(6)：622-626
[48]Doyle J J. Doyle J L Isolation of plant DNA from fresh tissue [J], Focus,1990, 12:13-15
[49]张云.油茶遗传多样性及遗传性状的RAPD分析[D].福州：福建师范大学.2003
[50]黄永芳等.油茶种质资源遗传多样性分析[J].林业科学.2006,42(4)：38-43
[51]王保明等.应用ISSR分析油茶无性系的遗传多样性[J].东北林业大学报,2008,36(6)：19-23
[52]Ammiraju J S, Dholakia B B, Santra D K,et al.Identification of inter simple Sequence repeat(ISSR)markers associated with seed size in wheat[J].Theor Appl Genet,2001,102:726-732
[53]LinZ, ZhangX, NieY, HeD, Wu M. Construction of a genetic linkage map for Cotton based on SRAP [J]. Chinese Science Bulletin.2003,48(19):2063-2067
[54]Doyle J J. Doyle J L Isolation of plant DNA from fresh tissue [J], Focus,1990, 12:13-15
[55]郑婷婷,林萍,杨水平等.油茶SRAP-PCR反应体系的优化[J],林业科学研究,2010,23(2)：302-307
[56]Wirght S. The genetic structure of populations [M].AnnEugen,1951,15:323-350
[57]Bhatt.B.M. Multivariate analysis approach to selection of parents for hybridization aiming at yield improvement in self-pollinated crops[J]. Australian Journal of Agricultural Research,1970,21(1):1-7.
[58]毛盛贤,黄远樟.群体遗传及其程序设计[M].北京：农业出版社,1991.
[59]王玲,卓丽环,杨传平等.兴安落叶松等位酶水平的遗传多样性[J].林业科学,2009,45(8)：170-174
[60]George J, Dobrowolski M P,Van-Zijll-de-Jong E, et al. Assessment of genetic diversity in cultivars of white clover (Trifolium repens L.) detected by SSR polymorphisms [J].Genome,2006,49(8):919-930.
[61]Archak S,Gaikwad A B,Gautam D,et al. Comparative assessment of DNA fingerprinting techniques(RAPD,ISSR and AFLP) for genetic analysis of cashew(Anacardium occidentale L.) accessions of India[J].Genome,2003,46:362-369.
[62]Suyama Y. A cline of allozyme variation in Abies mariesii.[J].Journal of Plant Research,110:219-226
[63]Nybom H. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants [J]. Molecular Ecology,2004,13 (5):1143-1155
[64]黄武刚,程丽莉,周志军,刘建立.板栗野生居群遗传多样性研究[J].果树学报,2010,27(2)：227-232
[65]王滑,郝俊民,王宝庆,裴东.中国核桃8个天然居群遗传多样性分析[J].林业科学,2007,43(7)
[66]刘军,陈益泰,姜景民,何贵平,余国民.毛红椿群体遗传结构的SSR分析[J].林业科学研究2009,22(1)：37-41
[67]Hamrick J L, Godt M J W, Sherman-BroylesL S. Gene flow among plant populations:Evidence from genetic markers[C]//Hoch D C, Stephnon A G. Experimental and Molecular App roaches to Plant Biosystematics. Missouri Botanical Garden,1995,215-232
[68]李景秀等.云南紫金牛属植物资源调查研究[J].广西植物,2009,29(2)：236-241
[69]Lande R. Genetics and demography in biological conservation[J].Science,1988, 241:1455-1460
[70]唐芳,李小元,吴卫国,刘军鸽.山茶油脂肪酸甲酯化条件研究[J].粮食与油脂,2010(08)
[71]盛灵慧,黄峥,王晶.气相色谱质谱法测定植物油中脂肪酸[J].化学分析计量,2010,19(2)
[72]廖书娟,吉当玲,童华荣.茶油脂肪酸组成及其营养保健功能[J].粮食与油脂,2005(06)
[2]胡芳名,谭晓风,刘惠民.中国主要经济树种栽培与利用[M].北京：中国林业出版社,2006.
[3]欧阳嘉,曹叶青,樊可可,沈树宝.多种油脂的脂肪酸组成分析[J].南京化工大学学报(自然科学版),1997,(02).
[4]丛玲美,姚小华,费学谦,王开良,王亚萍,王年金.长期贮藏对茶油酸值和过氧化值的影响[J].林业科学研究,2007,(02)
[5]李纪元.种好旱地,粮油丰收[J].山西农业科学,1981(06)
[6]姚小华.油茶高效实用栽培技术[M].北京：科学出版社,2010.
[7]庄瑞林,王德斌,蔡肖群,匡元玉,熊年康.山茶大树嫁接的研究[J].经济林研究1994,12(2)
[8]周盛,朱金惠,肖景治等.油茶远缘杂交育种试验[J].经济林研究,2001,19(1)：20-25
[9]傅瑞树等.油茶某些生理活动的昼夜变化[J].林业科技通讯,1984,(8)：11-14
[10]李建安,张日清,石明旺,杨伟.油茶两物种花药培养愈伤组织诱导试验[J].经济林研究2003,21(3)：36-38
[11]闻丽,张日清,李建安,吕芳德.油荼种质改良现状及其花药培养技术的应用前景[J].经济林研究2004,22(4)：87-90
[12]张日清,闻丽,刘友全,李建安,漆龙霖,栗彬,张春生.低温预处理对油茶花药愈伤组织诱导的影响[J].中南林学院学报,2005(06)
[13]丁檀磊,张日清,刘友全,闻丽.油茶12个物种花药愈伤组织诱导及继代培养[J].经济林研究,2007,25(1)：20-24
[14]沈德绪,王元裕,陈力耕.柑橘遗传育种学[M].北京,科学出版社,1998
[15]杨学明,裔传灯.植物染色体原位杂交技术及其在稻属研究中的应用[J].植物遗传资源学报[J],.2004(04)
[16]张玉静.分子遗传学[M].北京：科学出版社,2002,9-46
[17]刘军,陈益泰,姜景民,何贵平,余国民.毛红椿群体遗传结构的SSR分析[J].林业科学研究,2009,22(1)：37-41
[18]Godwir I D, Aitke R E, Smith L W. Application of inter-simply sequence repeat (ISSR) markers to plant genetics [J]. Electrophoresis,1997,18:1524-1528
[19]Qian W, Hong D Y, Ge S. Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers [J]. Theor Appl Genet,2001,102:440-449
[20]刘平等.赞皇大枣的群体遗传多样性评价[J].果树学报,2006,23(5)：685-689
[21]Kantety R V, Rotal M L, Matthews D E, Sorrells M E. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat [J]. Plant Mol Biol,2002,48:501-510
[22]Femamdez M E, Figueiras A M, Benito C. The use of ISSR and RAPD markets for detecting DNA polymorphism, gene type identification and diversity among barley cultivars with known origin [J]. Theor Appl Genet,2002,104:845-851
[23]李斌,顾万春.白皮松保育遗传学——天然群体遗传多样性评价与保护策略[J].林业科学,2005,41(1)：57-64
[24]高秀琴,兰进好,林琪,等.部分旱地小麦品种(系)遗传多样性的SSR分析[J].麦类作物学报,2008,28(4)：577-581
[25]严学兵,王堑,周禾,等.不同来源SSR标记在我国披碱草属植物的通用性和效率评价[J].草业学报,2008,17(6)：112-120
[26]Amel S, Mikhtar T, Salwa Z, et al. Inter-simple sequence repeat fingerprint s to assess genetic diversity in trnisian fig (Ficus carica L.) germplasm[J]. Genetic Resources and Crop Evolution,2004,51:269-275.
[27]Hassanien H A, Gilbey J. Genetic diversity and differentiation of Niletilap ia (Oreochrom is niloticus) revealed by DNA microsatellites[J]. Aquaculture Research, 2005,36,(14):1450-1457
[28]EP.NieeseJ.I.HormazaGH.MeGranahan. Molecular characterization and genetic relatedness among walnut (Juglans regia L.) genotypes base on RAPD markers [J]. Euphytica,1998,101 (2):199-206
[29]Echt C S, Deverno L L, Anzidei M. Chlorop last microsatellites reveal population genetic diversity in red pine, Pinus resinosa Ait [J]. Molecular Ecology, 1998,7(3):307-316
[30]LI G, QUIROS C F. Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:It's application to mapping and gene tagging in Brassica [J]. Theoretical and Applied Genetics.2001.103:455-461
[31]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].北京：科学出版社,2002：1-9
[32]WANG Gang.Construction of a Cucumber Genetic Linkage Map with RAPD, SRAP and SSR Markers[J]. Journal of Shanghai Jiaotong University (Science).2005, 45-49.
[33]Ferriol M, PicoB,Nuez F. Genetic diversity of a germplasm collection of cucurbita pepa using SRAP and AFLP markers[J].Theor Appl Genet,2003,107:271-282.
[34]李廷春,樊洪泓,高正良,周应兵,杨华应.丹参遗传多样性的SRAP标记分析[J].核农学报,208(05)
[35]沈程文,黄建安等.利用SRAP和ISSR标记分析广东茶树种质资源的遗传多样性[J].核农学报,2010,24(5)：948-955
[36]李怀志,韩洪强,李维为等.利用SRAP技术评价茄子种质资源遗传多样性[A]：中国园艺学会十届会员代表大会暨学术讨论论文集,2007
[37]刘建丰,王志德,刘艳华,牟建民.应用SRAP标记研究烟草种质资源的遗传多样性[J].中国烟草科学,2007(05)
[38]路娟,张绍铃等.樱桃SRAP-PCR体系优化及其遗传多样性分析[J].果树学报2009,26(2)：163-169
[39]左丹丹,赵海涛等.蜡梅天然群体遗传多样性的SRAP标记分析[J].园艺学报,2009,36(8)：1197-1202
[40]张春雨等.新疆野苹果群体遗传结构和遗传多样性的SRAP分析[J].园艺学报,2009,36(1)：7-14
[41]凌瑶等.西南五省区及非洲野生狗牙根种质基于SRAP标记的遗传多样性分析[J].草业学报,2010,19(2)：196-203
[42]England P R, Usher A V, Whelan R J, et al. Microsatellite diversity and genetic structure of fragmented populations of the rare, fire-dependent shrub Grevillea m acleayana [J]. Molecular Ecology,2002,11 (6):967-977
[43]鄢家俊等.青藏高原东南缘老芒麦自然居群遗传多样性的SRAP和SSR分析[J].草业学报,2010,19(4)：122-134
[44]刘月光,滕永勇,潘辰,韩延闯等.应用SRAP标记对莲藕资源的聚类分析[J].氨基酸和生物资源,2006(01)
[45]于拴仓,柴敏,姜立纲.主要樱桃番茄品种的分子鉴别[J].华北农学报,2005(05)
[46]张建成,王传堂,焦坤,杨新道SRAP标记技术在花生种子纯度鉴定中的应用[J].中国农学通报,2005(12)
[47]林忠旭,张献龙,聂以春.新型标记SRAP在棉花F2分离群体及遗传多样性评价中的适用性分析[J].遗传学报,2004,31(6)：622-626
[48]Doyle J J. Doyle J L Isolation of plant DNA from fresh tissue [J], Focus,1990, 12:13-15
[49]张云.油茶遗传多样性及遗传性状的RAPD分析[D].福州：福建师范大学.2003
[50]黄永芳等.油茶种质资源遗传多样性分析[J].林业科学.2006,42(4)：38-43
[51]王保明等.应用ISSR分析油茶无性系的遗传多样性[J].东北林业大学报,2008,36(6)：19-23
[52]Ammiraju J S, Dholakia B B, Santra D K,et al.Identification of inter simple Sequence repeat(ISSR)markers associated with seed size in wheat[J].Theor Appl Genet,2001,102:726-732
[53]LinZ, ZhangX, NieY, HeD, Wu M. Construction of a genetic linkage map for Cotton based on SRAP [J]. Chinese Science Bulletin.2003,48(19):2063-2067
[54]Doyle J J. Doyle J L Isolation of plant DNA from fresh tissue [J], Focus,1990, 12:13-15
[55]郑婷婷,林萍,杨水平等.油茶SRAP-PCR反应体系的优化[J],林业科学研究,2010,23(2)：302-307
[56]Wirght S. The genetic structure of populations [M].AnnEugen,1951,15:323-350
[57]Bhatt.B.M. Multivariate analysis approach to selection of parents for hybridization aiming at yield improvement in self-pollinated crops[J]. Australian Journal of Agricultural Research,1970,21(1):1-7.
[58]毛盛贤,黄远樟.群体遗传及其程序设计[M].北京：农业出版社,1991.
[59]王玲,卓丽环,杨传平等.兴安落叶松等位酶水平的遗传多样性[J].林业科学,2009,45(8)：170-174
[60]George J, Dobrowolski M P,Van-Zijll-de-Jong E, et al. Assessment of genetic diversity in cultivars of white clover (Trifolium repens L.) detected by SSR polymorphisms [J].Genome,2006,49(8):919-930.
[61]Archak S,Gaikwad A B,Gautam D,et al. Comparative assessment of DNA fingerprinting techniques(RAPD,ISSR and AFLP) for genetic analysis of cashew(Anacardium occidentale L.) accessions of India[J].Genome,2003,46:362-369.
[62]Suyama Y. A cline of allozyme variation in Abies mariesii.[J].Journal of Plant Research,110:219-226
[63]Nybom H. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants [J]. Molecular Ecology,2004,13 (5):1143-1155
[64]黄武刚,程丽莉,周志军,刘建立.板栗野生居群遗传多样性研究[J].果树学报,2010,27(2)：227-232
[65]王滑,郝俊民,王宝庆,裴东.中国核桃8个天然居群遗传多样性分析[J].林业科学,2007,43(7)
[66]刘军,陈益泰,姜景民,何贵平,余国民.毛红椿群体遗传结构的SSR分析[J].林业科学研究2009,22(1)：37-41
[67]Hamrick J L, Godt M J W, Sherman-BroylesL S. Gene flow among plant populations:Evidence from genetic markers[C]//Hoch D C, Stephnon A G. Experimental and Molecular App roaches to Plant Biosystematics. Missouri Botanical Garden,1995,215-232
[68]李景秀等.云南紫金牛属植物资源调查研究[J].广西植物,2009,29(2)：236-241
[69]Lande R. Genetics and demography in biological conservation[J].Science,1988, 241:1455-1460
[70]唐芳,李小元,吴卫国,刘军鸽.山茶油脂肪酸甲酯化条件研究[J].粮食与油脂,2010(08)
[71]盛灵慧,黄峥,王晶.气相色谱质谱法测定植物油中脂肪酸[J].化学分析计量,2010,19(2)
[72]廖书娟,吉当玲,童华荣.茶油脂肪酸组成及其营养保健功能[J].粮食与油脂,2005(06)