硝化应激在心血管疾病中作用及机制的研究进展
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摘要
硝化应激(nitrative stress)指蛋白质的酪氨酸硝化,是一种重要的蛋白质翻译后修饰。近年来大量研究发现硝化应激在多种心血管疾病如缺血再灌注损伤、动脉粥样硬化、心力衰竭及高血压病等疾病的病理生理过程中起着关键作用。硝化应激是指由一氧化氮或由一氧化氮(?NO)衍生的活性氮族(reactive nitrogen species,RNS)与活性氧族共同联合发生的生物化学作用,使细胞内蛋白质中的酪氨酸硝化成3-硝基酪氨酸(3-nitrotyrosine,3-NT),引起细胞损伤或凋亡,从而对细胞产生多种毒性作用的病理状态,其最主要的两种途径是过氧亚硝酸根(ONOO-)途径和NO2-/H2O2/血红素过氧化物酶途径,反应为自由基机理。本文对硝化应激在多种心血管疾病发生发展中的作用及机制做了综述,指出蛋白质硝化具有选择性,特定酪氨酸残基发生硝化能够改变蛋白质的结构和功能,进一步鉴别体内的硝化蛋白,研究特定蛋白质硝化与疾病发生发展关系将为人类预防和治疗多种心血管疾病开辟新的途径。
Nitrative stress is an important posttranslational modification involving a variety of diseases.Many recent studies demonstrated that nitrative stress plays a key role in the pathophysiology of several major cardiovascular diseases such as myocardial ischemical reperfusion injury, atherosclerosis,heart failure,hypertension. nitration is a biological process derived from the biochemical interaction of nitric oxide or nitric oxide-derived secondary products ( RNS ) with reactive oxygen species,lead to the concomitant formation of tyrosyl radicals and?NO2,which combine at diffusion-controlled rates to form 3-nitrotyrosine,then lead cell damage or death,which cause many kinds of cytotoxic effects in cells.It is occurred via peroxynitrite or nitrite/hydrogen peroxide/hemeproxydase system,and nitrotyrosine is formed by free radical reaction. In this article,the role and mechanism of nitrative stress in several major cardiovascular diseases are reviewed.It points out that protein nitration has selectivity,and nitration of special tyrosine residue(s)can lead to the alteration of the structure and functions of the protein.Individual identification of nitrated proteins in vivo and investigation of the relation between special protein nitration and the development and progression of many cardiovascular diseases may open new avenues for the preventing and treatment of human cardiovascular diseases.
Nitrative stress is an important posttranslational modification involving a variety of diseases.Many recent studies demonstrated that nitrative stress plays a key role in the pathophysiology of several major cardiovascular diseases such as myocardial ischemical reperfusion injury, atherosclerosis,heart failure,hypertension. nitration is a biological process derived from the biochemical interaction of nitric oxide or nitric oxide-derived secondary products ( RNS ) with reactive oxygen species,lead to the concomitant formation of tyrosyl radicals and?NO2,which combine at diffusion-controlled rates to form 3-nitrotyrosine,then lead cell damage or death,which cause many kinds of cytotoxic effects in cells.It is occurred via peroxynitrite or nitrite/hydrogen peroxide/hemeproxydase system,and nitrotyrosine is formed by free radical reaction. In this article,the role and mechanism of nitrative stress in several major cardiovascular diseases are reviewed.It points out that protein nitration has selectivity,and nitration of special tyrosine residue(s)can lead to the alteration of the structure and functions of the protein.Individual identification of nitrated proteins in vivo and investigation of the relation between special protein nitration and the development and progression of many cardiovascular diseases may open new avenues for the preventing and treatment of human cardiovascular diseases.
引文
1 Ischiropoulos H.Biological tyrosine nitration:a athophysiological function of nitric oxide and reactive oxygen species[J].Arch Biochem Biophys,1998,356(1):1-11
2 Ohshima H,Friesen M,Brouet I,et al.Nitrotyrosine as a new marker for endogenous nitrosation and nitration of proteins [J].Food Chem Toxicol,1990,28(9):647-652
3 Ischiropoulos H, Zhu L,Chen J,et al.Peroxynitrite-mediated tyrosine nitration catalyzed by superoxide dismutase[J]. Arch Biochem Biophys,1992,298(2):431-437
4 Ye YZ,Strong M,Huang ZQ,et al.Antibodies that recognize nitrotyrosine[J].Methods Enzymol,1996,269:201-209
5 Greenacre SA,Ischiropoulos H.Tyrosine nitration: localization,quantification,consequences for protein function and signal transduction[J].Free Rad Res,2001, 34(6): 541-581
6 Turko IV,Murad F.Protein nitration in cardiovascular diseases[J]. Pharmacol Rev,2002,54(4):619-634
7 Davidge ST,Ojimba J,McLaughlin MK.Vascular function in the vitamin E-deprived rat.An interaction between nitric oxide and superoxide anions[J].Hypertension,1998,31:830-835
8 Frustaci A, Kajstura J, Chimenti C,et al.Myocardial cell death in human diabetes[J].Circ Res,2000,87(12):1123-1132
9 Kajstura J,Fiordaliso F,Andreoli AM,et al.IGF-1 overexpression inhibits the development of diabetic cardiomyopathy and angiotensin II-mediated oxidative stress[J].Diabetes,2001,50:1414-1424
10 Mihm MJ,Coyle CM,Schanbacher BL, et al.Peroxynitrite induced nitration and inactivation of myofibrillar creatine kinase in experimental heart failure[J].Cardiovasc Res, 2001,49(4):798-807
11 Zou MH,Leist M,Ullrich V.Selective nitration of prostacyclin synthase and defective vasorelaxation in atherosclerotic bovine coronary arteries[J].Am J Pathol,1999, 154(5):1359-1365
12 Turko IV,Marcondes S,Murad F.Diabetes-associated nitration of tyrosine and inactivation of succinyl-CoA:3-oxoacid CoA-transferase[J].Am J Physiol Heart Circ Physiol, 2001,281:H2289-H2294
13 Khan J,Brennan DM,Bradley N,et al.3-Nitrotyrosine in the proteins of human plasma determined by an ELISA method[J].Biochem J,1998,330:795-801
14 Marfella R, Quagliaro L, Nappo F, et al.Acute hyperglycemia induces an oxidative stress in healthy subjects[J]. J Clin Investig,2001,108(4): 635-636
15 Radi R.Nitric oxide,oxidants and protein tyrosine nitration[J]. Proc Natl Acad Sci U S A,2004,101(12):4003-4008
16 Ferdinandy P,Schulz R.Nitric oxide,superoxide,and peroxynitrite in myocardial ischaemia-reperfusion injury and preconditioning[J].Br J Pharmacol,2003,138(4):532-543
17 Hogg N,Singh R J, Kalyanaraman B.The role of glutathione in the transport and catabolism of nitric oxide[J].FEBS Lett,1996,382(3):223-228
18 Alvarez B,Radi R.Peroxynitrite reactivity with amino acids and proteins[J].Amino Acids,2003,25(3-4):295-311
19 Brennan ML,Wu W,Fu X,et al.A tale of two controversies: defining both the role of peroxidases in nitrotyrosine formation in vivo using eosinophil peroxidase and myeloperoxidase-deficient mice,and the nature of peroxidase-generated reactive nitrogen species[J].J Biol Chem,2002,277(20):17415-17427
20 Monzani E, Roncone R,Galliano M,et al.Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide[J].Eur J Biochem,2004,271(5):895-906
21 Thomas DD,Espey MG,Vitek MP,et al.Protein nitration ismediated by heme and free metals through Fenton-type chemistry: An alternative to the NO/O2-reaction[J].Proc Natl Acad Sci USA,2002,99(20):12691-12696
22 Bian K,Gao Z,Weisbrodt N,et al.The nature of heme/iron-induced protein tyrosine nitration[J].Proc Natl Acad Sci USA,2003,100(10):5712-5717
23 Pfeiffer S,Lass A,Schmidt K,et al.Protein Tyrosine nitration in cytokine-activated murine macrophages[J].Biol Chem,2001,276(36):34051-34058
24 Gunther MR,Hsi LC,Curtis JF,et al.Nitric oxide trapping of the tyrosyl radical of prostaglandin H synthase-2 leads to tyrosine iminoxyl radical and nitrotyrosine formation[J]. Biol Chem,1997,272(27):17086-17090
25 Ischiropoulos H.Biological selectivity and functional aspects of protein tyrosine nitration[J].Biochem Biophys Res Commun,2003,305(3):776-783
26 Batthyany C,Souza JM,Radi R,et al.Time course and site(s) of cytochrome c tyrosine nitration by peroxynitrite[J].B Biochemistry,2005,44:8038-8046
27 Gole MD,Souza JM,Choi I,et al.Plasma proteins modified by tyrosine nitration in acute respiratory distress syndrome[J].Am J Physiol Lung Cell Mol Physiol,2000, 278(5):L961-L967
28 Li H,Gutterman DD,Rusch NJ,et al.Nitration and functional loss of voltage-gated K+ channels in rat Coronary microvessels exposed to high glucose[J].Diabetes,2004,53:2436-2442
29 Reinehr R,Gorg B,Hongen A,et al.CD95-tyrosine nitration inhibits hyperosmotic and CD95 ligand-induced CD95 activation in rat hepatocytes[J].J Biol Chem,2004, 279(11):10364-10373
30 Takakura K,Beckman JS,MacMillan-Crow LA,et al.Rapid and irreversible inactivation of protein tyrosine phosphatases PTP1B, CD45,and LAR by peroxynitrite [J].Arch Biochem Biophys,1999,369(2):197-207
31 Mallozzi C,Di Stasi AM,Minetti M.Peroxynitrite modulates tyrosine-dependent signal transduction pathway of human erythrocyte band 3[J].Faseb J,1997,11(14):1281-1290
32 Jennings RB,Sommers HM,Smyth GA,et al.Myocardial necrosis induced by temporary occlusion of a coronary artery in the dog[J].Arch Pathol,1960,70:68-78
33 Ronson RS,Nakamura M,Vinten-Johansen J.The cardiovascular effects and implications of peroxynitrite[J]. Cardiovasc Res,1999,44(1):47-59
34 Ishid H,Ichimori K,Hirota Y,et al.Peroxynitrite-induced cardiac myocyte injury[J].Free Rad Biol Med,1996,20(3): 343-350
35 Ishida H,Genka C,Hirota Y,et al.Distinct roles of peroxynitrite and hydroxyl radical in triggering stunned myocardium-like impairment of cardiac myocytes in vitro[J].Mol Cell Biochem,1999,198(1-2):31-38
36 Liu P,Hock CE,Nagele R,et al.Formation of nitric oxide,superoxide, and peroxynitrite in myocardial ischemia-reperfusion injury in rats[J].Am J Physiol,1997,272(5Pt2): H2327-H2336
37 Huang CH,Vatner SF,Peppas AP,et al.Cardiac nerves affect myocardial stunning through reactive oxygen and nitric oxide mechanisms[J].Circ Res,2003,93(9):866-873
38 Pacher P,Liaudet L,Bai P,et al.Potent metalloporphyrin peroxynitrite decomposition catalyst protects against the development of doxorubicin-induced cardiac dysfunction [J]. Circulation,2003,107(6):896-904
39 Levrand S,Vannay-Bouchiche C,Pesse B,et al.Peroxynitrite is a major trigger of cardiomyocyte apoptosis in vitro and in vivo[J].Free Radic Biol Med,2006,41(6):886–895
40 Hayashi Y,Sawa Y,Fukuyama N,et al.Leukocyte-depleted terminal blood cardioplegia provides superior myocardial protective effects in association with myocardium-derived nitric oxide and peroxynitrite production for patients undergoing prolonged aortic crossclamping for more than
120 minutes[J].J Thorac Cardiovasc Surg,2003, 126(6):1813-1821
41 Mehlhorn U,Krahwinkel A,Geissler HJ,et al.Nitrotyrosine and 8-isoprostane formation indicate free radical-mediated injury in hearts of patients subjected to cardioplegia[J].J Thorac Cardiovasc Surg,2003,125(1):178-183
42 Hayashi Y,Sawa Y,Ohtake S,et al.Peroxynitrite formation from human myocardium after ischemia-reperfusion during open heart operation[J].Ann Thorac Surg,2001,72(2):571-576
43 Ravalli S,Albala A,Ming M,et al.Inducible nitric oxide synthase expression in smooth muscle cells and macrophages of human transplant coronary artery disease[J].Circulation,1998,97(23):2338-2345
44 Nakamura M,Thourani VH,Ronson RS,et al.Glutathione reverses endothelial damage fromperoxynitrite,the byproduct of nitric oxidedegradation,in crystalloid cardioplegia[J].Circulation,2000,102(19 Suppl 3):III332-338
45 Nossuli TO,Hayward R,Jensen D,et al.Mechanisms of cardioprotection by peroxynitrite in myocardial ischemia and reperfusion injury[J].Am J Physiol,1998,275(2Pt2): H509-H519
46 Schulz R,Dodge KL,Lopaschuk GD,et al.Peroxynitrite impairs cardiac contractile function by decreasing cardiac efficiency[J].Am J Physiol,1997,272(3Pt2):H1212-H1219
47 Ma XL,Gao F,Lopez BL,et al.Peroxynitrite,a Two-edged sword in post-ischemic myocardial Injury-dichotomy of Action in crystalloid-versus blood-perfused hearts[J].J Pharmacol Exp Ther,2000,292(3):912-920
48 Cheung PY,Wang W,Schulz R.Glutathione protects against myocardial ischemia-reperfusion injury bydetoxifying peroxynitrite[J].J Mol Cell Cardiol,2000, 32(9):1669-1678
49 Hansson GK.Inflammation,atherosclerosis,and coronary artery disease[J].N Engl J Med,2005,352(16):1685-1695
50 Rubbo H, O'Donnell V.Nitric oxide,peroxynitrite and lipoxygenase in atherogenesis:mechanistic insights[J]. Toxicology,2005,208(2): 305-317
51 Davis B,Zou MH.CD40 Ligand–dependent tyrosine nitration of prostacyclin synthase in vivo[J].Circulation, 2005,112(14):2184-2192
52 Pennathur S,Bergt C,Shao B,et al.Human atherosclerotic intima and blood of patients with established coronary artery disease contain high density lipoprotein damaged by reactive nitrogen species[J].J Biol Chem,2004,279(41): 42977-42983
53 Sucu N,Unlu A,Tamer L,et al.3-Nitrotyrosine in atherosclerotic blood vessels[J].Clin Chem Lab Med,2003, 41:23-25
54 Go YM,Patel RP,Maland MC,et al.Evidence for peroxynitrite as a signaling molecule in flow-dependent activation of c-Jun NH(2)-terminal kinase[J].Am J Physiol,1999,277(4 Pt2):H1647-1653
55 Pennathur S,Bergt C,Shao B,et al.Human atherosclerotic intima and blood of patients with established coronary artery disease contain high density lipoprotein damaged by reactive nitrogen species[J].J Biol Chem,2004,279(41):42977-42983
56 Rubbo H,Trostchansky A,Botti H,et al.Interactions of nitric oxide and peroxynitrite with low-density lipoprotein[J].Biol Chem,2002,383(3-4):547-552
57 Kano H,Hayashi T,Sumi D,et al.Food restriction attenuates age-related increase estriol retards and stabilizes atherosclerosis through an NO-mediated system[J].Life Sci,2002,71:31-42
58 Szilvassy Z,Csont T,Pali T,et al.Nitric oxide,peroxynitrite and cGMP in atherosclerosis-induced hypertension in rabbits:beneficial effects of cicletanine[J].J Vasc Res,2001, 38(1):39-46
59 Yang H,Shi M,Story J,et al.Food restriction attenuates age-related increase in the sensitivity of endothelial cells to oxidized lipids[J].J Gerontol A Biol Sci Med Sci,2004, 59(4):316-323
60 Bagi Z,Ungvari Z,Koller A.Xanthine oxidase-derived reactive oxygen species convert flow-induced arteriolar dilation to constriction in hyperhomocysteinemia: possible role of peroxynitrite[J].Arterioscler Thromb Vasc Biol,2002, 22(1):28-33
61 Hucks D,Thuraisingham RC,Raftery MJ,et al.Homocysteine induced impairment of nitric oxide-dependent vasorelaxation is reversible by the superoxide dismutase mimetic TEMPOL[J]. Nephrol Dial Transplant,2004,19(8): 1999-2005
62 Sood HS,Cox MJ,Tyagi SC.Generation of nitrotyrosine precedes activation of metalloproteinase in myocardium of hyperhomocysteinemic rats[J].Antioxid Redox Signal,2002, 4(5):799-804
63 Zhang R,Ma J,Xia M,et al.Mild hyperhomocysteinemia induced by feeding rats diets rich in methionine or deficient in folate promotes early atherosclerotic inflammatory processes[J].J Nutr,2004,134(4): 825-830
64 Mungrue,IN,Gros R,You X,et al.Cardiomyocyte overexpression of iNOS in mice results in peroxynitrite generation,heart block,and sudden death[J].J Clin Invest, 2002,109(6):735-743
65 Feng Q,Lu X,Jones DL,et al.Increased inducible nitric oxide synthase expression contributes to myocardial dysfunction and higher mortality after myocardial infarction in mice[J].Circulation,2001,104(6):700-704 66 Mihm MJ,Coyle CM,Schanbacher BL,et al.Peroxynitrite induced nitration and inactivation of myofibrillar creatine kinase in experimental heart failure[J].Cardiovasc Res,2001, 49 (4):798-807
67 Mihm MJ,Yu F,Carnes CA,et al.Impaired myofibrillar energetics and oxidative injury during human atrial fibrillation[J].Circulation, 2001,104(2):174-180
68 Okamoto T,Akaike T,Sawa T,et al.Activation of matrix metalloproteinases by peroxynitrite-induced protein S-glutathiolation via disulfide S-oxide formation[J].J BiolChem,2001,276(31):29596-29602
69 Lalu MM,Wang W,Schulz R,et al.Peroxynitrite in myocardial ischemia-reperfusion injury.Heart Fail Rev,2002, 7(4):359-369
70 Szabo C.Multiple pathways of peroxynitrite cytotoxicity[J]. Toxicol Lett,2003,140-141:105–112
71 Virag L,Szabo C.The therapeutic potential of poly(ADP-ribose) polymerase inhibitors[J].Pharmacol Rev,2002,54(3): 375-429
72 Spinale,FG.Matrix metalloproteinases:regulation and dysregulation in the failing heart[J].Circ Res,2002,90(5): 520-530
73 Siwik DA,Colucci WS.Regulation of matrix metalloproteinases by cytokines and reactive oxygen/ nitrogen species in the myocardium[J].Heart Fail Rev,2004, 9(1):43-51
74 Wang W,Sawicki G,Schulz R.Peroxynitrite-induced myocardial injury is mediated through matrix metalloproteinase-2[J].Cardiovasc Res,2002,53(1):165-174
75 Peterson JT,Hallak H,Johnson L,et al.Matrix metalloproteinase inhibition attenuates left ventricular remodeling and dysfunction in a rat model of progressive heart failure[J].Circulation,2001,103(18):2303-2309
76 Gao CQ,Sawicki G,Suarez-Pinzon WL,et al.Matrix metalloproteinase-2 mediates cytokine-induced myocardial contractile dysfunction[J].Cardiovasc Res,2003,57(2):426-433
77 Pacher P,Liaudet L,Bai P,et al.Potent metalloporphyrin peroxynitrite decomposition catalyst protects against the development of doxorubicin-induced cardiac dysfunction [J].Circulation,2003,107(6):896-904
78 Bai P,Mabley JG,Liaudet L,et al.Matrix metalloproteinase activation is an early event in doxorubicin-induced cardiotoxicity[J].Oncol Rep,2004,11(2):505-508
79 Docherty JC,Kuzio B,Silvester JA,et al.An inhibitor of poly (ADP-ribose) synthetase activity reduces contractile dysfunction and preserves high energy phosphate levels during reperfusion of the ischaemic rat heart[J].Br J Pharmacol,1999,127(6):1518-1524
80 Pacher P,Liaudet L,Soriano FG,et al.The role of poly(ADP-ribose) polymerase activation in the development of myocardial and endothelial dysfunction in diabetes[J]. Diabetes,2002,51(2):514-521
81 Pillai JB,Russell HM,Jeevanandam JRV,et al.Increased expression of poly (ADP) ribose polymerase-1 contributes to caspase-independent myocyte cell death during heart failure[J].Am J Physiol Heart Circ Physiol,2005,288(2): H486-H496
82 Pacher P,Liaudet L,Bai P,et al.Activation of poly(ADP-ribose) polymerase contributes to development of doxorubicin-induced heart failure[J].J Pharmacol Exp Ther.2002,300(3):862-867
83 Xiao CY,Chen M,Zsengeller Z,et al.Poly(ADP-ribose) polymerase promotes cardiac remodeling,contractile failure and translocation of apoptosis-inducing factor in a murine experimental model of aortic banding and heart failure[J].J Pharmacol Exp Ther,2005,312(3):891-898
84 Wattanapitayakul SK,Weinstein DM,Holycross BJ,et al. Endothelial dysfunction and peroxynitrite formation are early events in angiotensin-induced cardiovascular disorders[J].FASEB J,2000,14(2):271-278
85 Szabo C,Pacher P,Zsengeller Z,et al.Angiotensin II-mediated endothelial dysfunction: role of poly(ADP-ribose) polymerase activation[J].Mol Med,2004,10(1-6):28-35
86 Minchenko, AG,Stevens MJ,White L,et al.Diabetes-induced overexpression of endothelin-1 and endothelin receptors in the rat renal cortex is mediated via poly(ADP-ribose)polymerase activation[J].FASEB J,2003, 17(11): 1514-1516
87 Bai P,Mabley JG,Liaudet L,et al.Matrix metalloproteinase activation is an early event in doxorubicin-induced cardiotoxicity[J].Oncol Rep,2004,11(2):505-508
88 Pacher P,Liaudet L,Bai P,et al.Potent metalloporphyrin peroxynitrite decomposition catalyst protects against the development of doxorubicin-induced cardiac dysfunction[J]. Circulation,2003,107(6): 896-904
89 Feng Q,Lu X,Jones DL,et al.Increased inducible nitric oxide synthase expression contributes to myocardialdysfunction and higher mortality after myocardial infarction in mice[J].Circulation,2001,104(6):700-704
90 Lokuta AJ,Maertz NA, Meethal SV,et al.Increased Nitration of Sarcoplasmic Reticulum Ca2+-ATPase in Human Heart Failure[J]. Circulation,2005,111(8):988-995
91 Bouloumie A,Bauersachs J,Linz W,et al.Endothelial dysfunction coincides with an enhanced nitric oxide synthase expression and superoxide anion production[J]. Hypertension,1997,30:934-941
92 Calo L,Giacon B,Davis PA,et al.Oxidative stress and TGFbeta in kidney-transplanted patients with cyclosporin-induced hypertension. Effect of carvedilol and nifedipine[J]. Clin Nephrol, 2002,58(2):103-110
93 Dobrian AD,Schriver SD,Prewitt RL.Role of angiotensin II and free radicals in blood pressure regulation in a rat model of renal hypertension[J].Hypertension,2001,38(3):361-366
94 Vaziri ND,Ni Z,Oveisi F,et al.Enhanced nitric oxide inactivation and protein nitration by reactive oxygen species in renal insufficiency.Hypertension,2002,39(1):135-141
95 Van der Loo B,Labugger R,Skepper JN,et al.Enhanced peroxynitrite formation is associated with vascular aging[J]. J Exp Med,2000,192(12):1731-1744
96 Knyushko TV,Sharov VS,Williams TD,et al.3-Nitrotyrosine modification of SERCA2a in the agingheart:a distinct signature of the cellular redox environment[J]. Biochemistry, 2005,44(39): 13071-13081
97 Kooy NW,Lewis SJ,Royall JA,et al.Extensive tyrosine nitration in human myocardial inflammation: Evidence for the presence of peroxynitrite[J].Crit Care Med,1997,25(5): 812-819
98 Mebazaa A,De Keulenaer GW,Paqueron X,et al. Activation of cardiac endothelium as a compensatory component in endotoxin-induced cardiomyopathy[J]. Circulation,2001, 104(25): 3137-3144
99 Pignatelli B,Li CQ,Boffetta P,et al.Nitrated and oxidized plasma proteins in smokers and lung cancer patients[J]. Cancer Res, 2001,61(2):778-784
100 Cynthia A,Knight-Lozano BS,Christal G,et al.Cigarette smoke exposure and hypercholesterolemia increase mitochondrial damage in cardiovascular tissues[J]. Circulation,2002,105(7):849-854
101 Ishiyama S,Hiroe M,Nishikawa T,et al.Nitric oxide contributes to the progression of myocardial damage in experimental autoimmune myocarditis in rats[J]. Circulation, 1997,95(2):489-496
102 Bachmaier K, Neu N, Pummerer C,et al.iNOS expression and nitrotyrosine formation in the myocardium in response to inflammation is controlled by the interferon regulatory transcription factor[J].Circulation,1997,96(2):585-591
103 Ceriello A,Mercuri F,Quagliaro L,et al.Detection ofnitrotyrosine in the diabetic plasma:evidence of oxidative stress[J].Diabetologia,2001,44(7):834-838
104 Wang XL,Rainwater DL,Leone A,et al.Effects of diabetes on plasma nitrotyrosine levels[J].Diabet Med,2004, 21(6):577-580
105 Turko IV,Aulak KS,Stuehr DJ,et al.Protein tyrosine nitration in the mitochondria from diabetic mouse heart[J].J Biol Chem,2003,278(36):33972-33977
106 Hermo R,Cristina M,Mary Mazzotta,et al.Circulating levels of nitrated apolipoprotein A-I are increased in type 2 diabetic patients[J].Clin Chem Lab Med,2005,43(6):601-606
2 Ohshima H,Friesen M,Brouet I,et al.Nitrotyrosine as a new marker for endogenous nitrosation and nitration of proteins [J].Food Chem Toxicol,1990,28(9):647-652
3 Ischiropoulos H, Zhu L,Chen J,et al.Peroxynitrite-mediated tyrosine nitration catalyzed by superoxide dismutase[J]. Arch Biochem Biophys,1992,298(2):431-437
4 Ye YZ,Strong M,Huang ZQ,et al.Antibodies that recognize nitrotyrosine[J].Methods Enzymol,1996,269:201-209
5 Greenacre SA,Ischiropoulos H.Tyrosine nitration: localization,quantification,consequences for protein function and signal transduction[J].Free Rad Res,2001, 34(6): 541-581
6 Turko IV,Murad F.Protein nitration in cardiovascular diseases[J]. Pharmacol Rev,2002,54(4):619-634
7 Davidge ST,Ojimba J,McLaughlin MK.Vascular function in the vitamin E-deprived rat.An interaction between nitric oxide and superoxide anions[J].Hypertension,1998,31:830-835
8 Frustaci A, Kajstura J, Chimenti C,et al.Myocardial cell death in human diabetes[J].Circ Res,2000,87(12):1123-1132
9 Kajstura J,Fiordaliso F,Andreoli AM,et al.IGF-1 overexpression inhibits the development of diabetic cardiomyopathy and angiotensin II-mediated oxidative stress[J].Diabetes,2001,50:1414-1424
10 Mihm MJ,Coyle CM,Schanbacher BL, et al.Peroxynitrite induced nitration and inactivation of myofibrillar creatine kinase in experimental heart failure[J].Cardiovasc Res, 2001,49(4):798-807
11 Zou MH,Leist M,Ullrich V.Selective nitration of prostacyclin synthase and defective vasorelaxation in atherosclerotic bovine coronary arteries[J].Am J Pathol,1999, 154(5):1359-1365
12 Turko IV,Marcondes S,Murad F.Diabetes-associated nitration of tyrosine and inactivation of succinyl-CoA:3-oxoacid CoA-transferase[J].Am J Physiol Heart Circ Physiol, 2001,281:H2289-H2294
13 Khan J,Brennan DM,Bradley N,et al.3-Nitrotyrosine in the proteins of human plasma determined by an ELISA method[J].Biochem J,1998,330:795-801
14 Marfella R, Quagliaro L, Nappo F, et al.Acute hyperglycemia induces an oxidative stress in healthy subjects[J]. J Clin Investig,2001,108(4): 635-636
15 Radi R.Nitric oxide,oxidants and protein tyrosine nitration[J]. Proc Natl Acad Sci U S A,2004,101(12):4003-4008
16 Ferdinandy P,Schulz R.Nitric oxide,superoxide,and peroxynitrite in myocardial ischaemia-reperfusion injury and preconditioning[J].Br J Pharmacol,2003,138(4):532-543
17 Hogg N,Singh R J, Kalyanaraman B.The role of glutathione in the transport and catabolism of nitric oxide[J].FEBS Lett,1996,382(3):223-228
18 Alvarez B,Radi R.Peroxynitrite reactivity with amino acids and proteins[J].Amino Acids,2003,25(3-4):295-311
19 Brennan ML,Wu W,Fu X,et al.A tale of two controversies: defining both the role of peroxidases in nitrotyrosine formation in vivo using eosinophil peroxidase and myeloperoxidase-deficient mice,and the nature of peroxidase-generated reactive nitrogen species[J].J Biol Chem,2002,277(20):17415-17427
20 Monzani E, Roncone R,Galliano M,et al.Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide[J].Eur J Biochem,2004,271(5):895-906
21 Thomas DD,Espey MG,Vitek MP,et al.Protein nitration ismediated by heme and free metals through Fenton-type chemistry: An alternative to the NO/O2-reaction[J].Proc Natl Acad Sci USA,2002,99(20):12691-12696
22 Bian K,Gao Z,Weisbrodt N,et al.The nature of heme/iron-induced protein tyrosine nitration[J].Proc Natl Acad Sci USA,2003,100(10):5712-5717
23 Pfeiffer S,Lass A,Schmidt K,et al.Protein Tyrosine nitration in cytokine-activated murine macrophages[J].Biol Chem,2001,276(36):34051-34058
24 Gunther MR,Hsi LC,Curtis JF,et al.Nitric oxide trapping of the tyrosyl radical of prostaglandin H synthase-2 leads to tyrosine iminoxyl radical and nitrotyrosine formation[J]. Biol Chem,1997,272(27):17086-17090
25 Ischiropoulos H.Biological selectivity and functional aspects of protein tyrosine nitration[J].Biochem Biophys Res Commun,2003,305(3):776-783
26 Batthyany C,Souza JM,Radi R,et al.Time course and site(s) of cytochrome c tyrosine nitration by peroxynitrite[J].B Biochemistry,2005,44:8038-8046
27 Gole MD,Souza JM,Choi I,et al.Plasma proteins modified by tyrosine nitration in acute respiratory distress syndrome[J].Am J Physiol Lung Cell Mol Physiol,2000, 278(5):L961-L967
28 Li H,Gutterman DD,Rusch NJ,et al.Nitration and functional loss of voltage-gated K+ channels in rat Coronary microvessels exposed to high glucose[J].Diabetes,2004,53:2436-2442
29 Reinehr R,Gorg B,Hongen A,et al.CD95-tyrosine nitration inhibits hyperosmotic and CD95 ligand-induced CD95 activation in rat hepatocytes[J].J Biol Chem,2004, 279(11):10364-10373
30 Takakura K,Beckman JS,MacMillan-Crow LA,et al.Rapid and irreversible inactivation of protein tyrosine phosphatases PTP1B, CD45,and LAR by peroxynitrite [J].Arch Biochem Biophys,1999,369(2):197-207
31 Mallozzi C,Di Stasi AM,Minetti M.Peroxynitrite modulates tyrosine-dependent signal transduction pathway of human erythrocyte band 3[J].Faseb J,1997,11(14):1281-1290
32 Jennings RB,Sommers HM,Smyth GA,et al.Myocardial necrosis induced by temporary occlusion of a coronary artery in the dog[J].Arch Pathol,1960,70:68-78
33 Ronson RS,Nakamura M,Vinten-Johansen J.The cardiovascular effects and implications of peroxynitrite[J]. Cardiovasc Res,1999,44(1):47-59
34 Ishid H,Ichimori K,Hirota Y,et al.Peroxynitrite-induced cardiac myocyte injury[J].Free Rad Biol Med,1996,20(3): 343-350
35 Ishida H,Genka C,Hirota Y,et al.Distinct roles of peroxynitrite and hydroxyl radical in triggering stunned myocardium-like impairment of cardiac myocytes in vitro[J].Mol Cell Biochem,1999,198(1-2):31-38
36 Liu P,Hock CE,Nagele R,et al.Formation of nitric oxide,superoxide, and peroxynitrite in myocardial ischemia-reperfusion injury in rats[J].Am J Physiol,1997,272(5Pt2): H2327-H2336
37 Huang CH,Vatner SF,Peppas AP,et al.Cardiac nerves affect myocardial stunning through reactive oxygen and nitric oxide mechanisms[J].Circ Res,2003,93(9):866-873
38 Pacher P,Liaudet L,Bai P,et al.Potent metalloporphyrin peroxynitrite decomposition catalyst protects against the development of doxorubicin-induced cardiac dysfunction [J]. Circulation,2003,107(6):896-904
39 Levrand S,Vannay-Bouchiche C,Pesse B,et al.Peroxynitrite is a major trigger of cardiomyocyte apoptosis in vitro and in vivo[J].Free Radic Biol Med,2006,41(6):886–895
40 Hayashi Y,Sawa Y,Fukuyama N,et al.Leukocyte-depleted terminal blood cardioplegia provides superior myocardial protective effects in association with myocardium-derived nitric oxide and peroxynitrite production for patients undergoing prolonged aortic crossclamping for more than
120 minutes[J].J Thorac Cardiovasc Surg,2003, 126(6):1813-1821
41 Mehlhorn U,Krahwinkel A,Geissler HJ,et al.Nitrotyrosine and 8-isoprostane formation indicate free radical-mediated injury in hearts of patients subjected to cardioplegia[J].J Thorac Cardiovasc Surg,2003,125(1):178-183
42 Hayashi Y,Sawa Y,Ohtake S,et al.Peroxynitrite formation from human myocardium after ischemia-reperfusion during open heart operation[J].Ann Thorac Surg,2001,72(2):571-576
43 Ravalli S,Albala A,Ming M,et al.Inducible nitric oxide synthase expression in smooth muscle cells and macrophages of human transplant coronary artery disease[J].Circulation,1998,97(23):2338-2345
44 Nakamura M,Thourani VH,Ronson RS,et al.Glutathione reverses endothelial damage fromperoxynitrite,the byproduct of nitric oxidedegradation,in crystalloid cardioplegia[J].Circulation,2000,102(19 Suppl 3):III332-338
45 Nossuli TO,Hayward R,Jensen D,et al.Mechanisms of cardioprotection by peroxynitrite in myocardial ischemia and reperfusion injury[J].Am J Physiol,1998,275(2Pt2): H509-H519
46 Schulz R,Dodge KL,Lopaschuk GD,et al.Peroxynitrite impairs cardiac contractile function by decreasing cardiac efficiency[J].Am J Physiol,1997,272(3Pt2):H1212-H1219
47 Ma XL,Gao F,Lopez BL,et al.Peroxynitrite,a Two-edged sword in post-ischemic myocardial Injury-dichotomy of Action in crystalloid-versus blood-perfused hearts[J].J Pharmacol Exp Ther,2000,292(3):912-920
48 Cheung PY,Wang W,Schulz R.Glutathione protects against myocardial ischemia-reperfusion injury bydetoxifying peroxynitrite[J].J Mol Cell Cardiol,2000, 32(9):1669-1678
49 Hansson GK.Inflammation,atherosclerosis,and coronary artery disease[J].N Engl J Med,2005,352(16):1685-1695
50 Rubbo H, O'Donnell V.Nitric oxide,peroxynitrite and lipoxygenase in atherogenesis:mechanistic insights[J]. Toxicology,2005,208(2): 305-317
51 Davis B,Zou MH.CD40 Ligand–dependent tyrosine nitration of prostacyclin synthase in vivo[J].Circulation, 2005,112(14):2184-2192
52 Pennathur S,Bergt C,Shao B,et al.Human atherosclerotic intima and blood of patients with established coronary artery disease contain high density lipoprotein damaged by reactive nitrogen species[J].J Biol Chem,2004,279(41): 42977-42983
53 Sucu N,Unlu A,Tamer L,et al.3-Nitrotyrosine in atherosclerotic blood vessels[J].Clin Chem Lab Med,2003, 41:23-25
54 Go YM,Patel RP,Maland MC,et al.Evidence for peroxynitrite as a signaling molecule in flow-dependent activation of c-Jun NH(2)-terminal kinase[J].Am J Physiol,1999,277(4 Pt2):H1647-1653
55 Pennathur S,Bergt C,Shao B,et al.Human atherosclerotic intima and blood of patients with established coronary artery disease contain high density lipoprotein damaged by reactive nitrogen species[J].J Biol Chem,2004,279(41):42977-42983
56 Rubbo H,Trostchansky A,Botti H,et al.Interactions of nitric oxide and peroxynitrite with low-density lipoprotein[J].Biol Chem,2002,383(3-4):547-552
57 Kano H,Hayashi T,Sumi D,et al.Food restriction attenuates age-related increase estriol retards and stabilizes atherosclerosis through an NO-mediated system[J].Life Sci,2002,71:31-42
58 Szilvassy Z,Csont T,Pali T,et al.Nitric oxide,peroxynitrite and cGMP in atherosclerosis-induced hypertension in rabbits:beneficial effects of cicletanine[J].J Vasc Res,2001, 38(1):39-46
59 Yang H,Shi M,Story J,et al.Food restriction attenuates age-related increase in the sensitivity of endothelial cells to oxidized lipids[J].J Gerontol A Biol Sci Med Sci,2004, 59(4):316-323
60 Bagi Z,Ungvari Z,Koller A.Xanthine oxidase-derived reactive oxygen species convert flow-induced arteriolar dilation to constriction in hyperhomocysteinemia: possible role of peroxynitrite[J].Arterioscler Thromb Vasc Biol,2002, 22(1):28-33
61 Hucks D,Thuraisingham RC,Raftery MJ,et al.Homocysteine induced impairment of nitric oxide-dependent vasorelaxation is reversible by the superoxide dismutase mimetic TEMPOL[J]. Nephrol Dial Transplant,2004,19(8): 1999-2005
62 Sood HS,Cox MJ,Tyagi SC.Generation of nitrotyrosine precedes activation of metalloproteinase in myocardium of hyperhomocysteinemic rats[J].Antioxid Redox Signal,2002, 4(5):799-804
63 Zhang R,Ma J,Xia M,et al.Mild hyperhomocysteinemia induced by feeding rats diets rich in methionine or deficient in folate promotes early atherosclerotic inflammatory processes[J].J Nutr,2004,134(4): 825-830
64 Mungrue,IN,Gros R,You X,et al.Cardiomyocyte overexpression of iNOS in mice results in peroxynitrite generation,heart block,and sudden death[J].J Clin Invest, 2002,109(6):735-743
65 Feng Q,Lu X,Jones DL,et al.Increased inducible nitric oxide synthase expression contributes to myocardial dysfunction and higher mortality after myocardial infarction in mice[J].Circulation,2001,104(6):700-704 66 Mihm MJ,Coyle CM,Schanbacher BL,et al.Peroxynitrite induced nitration and inactivation of myofibrillar creatine kinase in experimental heart failure[J].Cardiovasc Res,2001, 49 (4):798-807
67 Mihm MJ,Yu F,Carnes CA,et al.Impaired myofibrillar energetics and oxidative injury during human atrial fibrillation[J].Circulation, 2001,104(2):174-180
68 Okamoto T,Akaike T,Sawa T,et al.Activation of matrix metalloproteinases by peroxynitrite-induced protein S-glutathiolation via disulfide S-oxide formation[J].J BiolChem,2001,276(31):29596-29602
69 Lalu MM,Wang W,Schulz R,et al.Peroxynitrite in myocardial ischemia-reperfusion injury.Heart Fail Rev,2002, 7(4):359-369
70 Szabo C.Multiple pathways of peroxynitrite cytotoxicity[J]. Toxicol Lett,2003,140-141:105–112
71 Virag L,Szabo C.The therapeutic potential of poly(ADP-ribose) polymerase inhibitors[J].Pharmacol Rev,2002,54(3): 375-429
72 Spinale,FG.Matrix metalloproteinases:regulation and dysregulation in the failing heart[J].Circ Res,2002,90(5): 520-530
73 Siwik DA,Colucci WS.Regulation of matrix metalloproteinases by cytokines and reactive oxygen/ nitrogen species in the myocardium[J].Heart Fail Rev,2004, 9(1):43-51
74 Wang W,Sawicki G,Schulz R.Peroxynitrite-induced myocardial injury is mediated through matrix metalloproteinase-2[J].Cardiovasc Res,2002,53(1):165-174
75 Peterson JT,Hallak H,Johnson L,et al.Matrix metalloproteinase inhibition attenuates left ventricular remodeling and dysfunction in a rat model of progressive heart failure[J].Circulation,2001,103(18):2303-2309
76 Gao CQ,Sawicki G,Suarez-Pinzon WL,et al.Matrix metalloproteinase-2 mediates cytokine-induced myocardial contractile dysfunction[J].Cardiovasc Res,2003,57(2):426-433
77 Pacher P,Liaudet L,Bai P,et al.Potent metalloporphyrin peroxynitrite decomposition catalyst protects against the development of doxorubicin-induced cardiac dysfunction [J].Circulation,2003,107(6):896-904
78 Bai P,Mabley JG,Liaudet L,et al.Matrix metalloproteinase activation is an early event in doxorubicin-induced cardiotoxicity[J].Oncol Rep,2004,11(2):505-508
79 Docherty JC,Kuzio B,Silvester JA,et al.An inhibitor of poly (ADP-ribose) synthetase activity reduces contractile dysfunction and preserves high energy phosphate levels during reperfusion of the ischaemic rat heart[J].Br J Pharmacol,1999,127(6):1518-1524
80 Pacher P,Liaudet L,Soriano FG,et al.The role of poly(ADP-ribose) polymerase activation in the development of myocardial and endothelial dysfunction in diabetes[J]. Diabetes,2002,51(2):514-521
81 Pillai JB,Russell HM,Jeevanandam JRV,et al.Increased expression of poly (ADP) ribose polymerase-1 contributes to caspase-independent myocyte cell death during heart failure[J].Am J Physiol Heart Circ Physiol,2005,288(2): H486-H496
82 Pacher P,Liaudet L,Bai P,et al.Activation of poly(ADP-ribose) polymerase contributes to development of doxorubicin-induced heart failure[J].J Pharmacol Exp Ther.2002,300(3):862-867
83 Xiao CY,Chen M,Zsengeller Z,et al.Poly(ADP-ribose) polymerase promotes cardiac remodeling,contractile failure and translocation of apoptosis-inducing factor in a murine experimental model of aortic banding and heart failure[J].J Pharmacol Exp Ther,2005,312(3):891-898
84 Wattanapitayakul SK,Weinstein DM,Holycross BJ,et al. Endothelial dysfunction and peroxynitrite formation are early events in angiotensin-induced cardiovascular disorders[J].FASEB J,2000,14(2):271-278
85 Szabo C,Pacher P,Zsengeller Z,et al.Angiotensin II-mediated endothelial dysfunction: role of poly(ADP-ribose) polymerase activation[J].Mol Med,2004,10(1-6):28-35
86 Minchenko, AG,Stevens MJ,White L,et al.Diabetes-induced overexpression of endothelin-1 and endothelin receptors in the rat renal cortex is mediated via poly(ADP-ribose)polymerase activation[J].FASEB J,2003, 17(11): 1514-1516
87 Bai P,Mabley JG,Liaudet L,et al.Matrix metalloproteinase activation is an early event in doxorubicin-induced cardiotoxicity[J].Oncol Rep,2004,11(2):505-508
88 Pacher P,Liaudet L,Bai P,et al.Potent metalloporphyrin peroxynitrite decomposition catalyst protects against the development of doxorubicin-induced cardiac dysfunction[J]. Circulation,2003,107(6): 896-904
89 Feng Q,Lu X,Jones DL,et al.Increased inducible nitric oxide synthase expression contributes to myocardialdysfunction and higher mortality after myocardial infarction in mice[J].Circulation,2001,104(6):700-704
90 Lokuta AJ,Maertz NA, Meethal SV,et al.Increased Nitration of Sarcoplasmic Reticulum Ca2+-ATPase in Human Heart Failure[J]. Circulation,2005,111(8):988-995
91 Bouloumie A,Bauersachs J,Linz W,et al.Endothelial dysfunction coincides with an enhanced nitric oxide synthase expression and superoxide anion production[J]. Hypertension,1997,30:934-941
92 Calo L,Giacon B,Davis PA,et al.Oxidative stress and TGFbeta in kidney-transplanted patients with cyclosporin-induced hypertension. Effect of carvedilol and nifedipine[J]. Clin Nephrol, 2002,58(2):103-110
93 Dobrian AD,Schriver SD,Prewitt RL.Role of angiotensin II and free radicals in blood pressure regulation in a rat model of renal hypertension[J].Hypertension,2001,38(3):361-366
94 Vaziri ND,Ni Z,Oveisi F,et al.Enhanced nitric oxide inactivation and protein nitration by reactive oxygen species in renal insufficiency.Hypertension,2002,39(1):135-141
95 Van der Loo B,Labugger R,Skepper JN,et al.Enhanced peroxynitrite formation is associated with vascular aging[J]. J Exp Med,2000,192(12):1731-1744
96 Knyushko TV,Sharov VS,Williams TD,et al.3-Nitrotyrosine modification of SERCA2a in the agingheart:a distinct signature of the cellular redox environment[J]. Biochemistry, 2005,44(39): 13071-13081
97 Kooy NW,Lewis SJ,Royall JA,et al.Extensive tyrosine nitration in human myocardial inflammation: Evidence for the presence of peroxynitrite[J].Crit Care Med,1997,25(5): 812-819
98 Mebazaa A,De Keulenaer GW,Paqueron X,et al. Activation of cardiac endothelium as a compensatory component in endotoxin-induced cardiomyopathy[J]. Circulation,2001, 104(25): 3137-3144
99 Pignatelli B,Li CQ,Boffetta P,et al.Nitrated and oxidized plasma proteins in smokers and lung cancer patients[J]. Cancer Res, 2001,61(2):778-784
100 Cynthia A,Knight-Lozano BS,Christal G,et al.Cigarette smoke exposure and hypercholesterolemia increase mitochondrial damage in cardiovascular tissues[J]. Circulation,2002,105(7):849-854
101 Ishiyama S,Hiroe M,Nishikawa T,et al.Nitric oxide contributes to the progression of myocardial damage in experimental autoimmune myocarditis in rats[J]. Circulation, 1997,95(2):489-496
102 Bachmaier K, Neu N, Pummerer C,et al.iNOS expression and nitrotyrosine formation in the myocardium in response to inflammation is controlled by the interferon regulatory transcription factor[J].Circulation,1997,96(2):585-591
103 Ceriello A,Mercuri F,Quagliaro L,et al.Detection ofnitrotyrosine in the diabetic plasma:evidence of oxidative stress[J].Diabetologia,2001,44(7):834-838
104 Wang XL,Rainwater DL,Leone A,et al.Effects of diabetes on plasma nitrotyrosine levels[J].Diabet Med,2004, 21(6):577-580
105 Turko IV,Aulak KS,Stuehr DJ,et al.Protein tyrosine nitration in the mitochondria from diabetic mouse heart[J].J Biol Chem,2003,278(36):33972-33977
106 Hermo R,Cristina M,Mary Mazzotta,et al.Circulating levels of nitrated apolipoprotein A-I are increased in type 2 diabetic patients[J].Clin Chem Lab Med,2005,43(6):601-606