白菜雄性不育相关新基因BcMF1的克隆和功能验证
|
摘要
芸薹属是十字花科植物的重要类群，也是我国栽培面积最大的蔬菜作物。白菜(Brassica campestrisL．ssp．chinensis Makino)是芸薹属芸薹种的一个亚种，是典型的异花授粉作物，具有显著的杂种优势，F_1代杂种的推广应用可以提高白菜的生产水平。雄性不育材料在白菜F_1代种子的生产中具有重要地位，其选育和利用日益受到人们的重视。以白菜雄性不育材料为基础，分析雄性不育相关基因的表达特征，分离雄性不育相关基因并验证基因的功能，不仅可以促进白菜雄性不育材料的选育，而且对于揭示植物雄性不育的分子机制也具有重要的理论意义。
本文以‘矮脚黄’白菜隐性核雄性不育两用系为研究对象，在前人获得雄性不育相关新基因Brassica campestris Male Fertility 1(BcMF1)的cDNA拼接序列的基础上，通过PCR进一步克隆BcMF1的DNA全长和cDNA全长；通过TAIL PCR技术克隆该基因的启动子序列；对BcMF1及其启动子的序列特征以及编码蛋白的功能进行分析和预测；并在几种芸薹属植物中进行BcMF1同源基因的克隆和进化分析。为验证BcMF1的功能，构建了RNA干涉(RNAi)和反义RNA植物表达载体，在分析菜心BcMF1同源基因表达特征的基础上，通过农杆菌介导法转化菜心植株，获取BcMF1表达沉默的卡那霉素抗性植株，对转基因植株的花粉发育状态进行观察，深入分析BcMF1在白菜花粉发育中的生物学功能。本研究获得的主要结果如下：
(1)根据表达差异片段BcMF-A15T17及其RACE扩增序列设计引物，PCR扩增得到BcMF1准确的cDNA全长和DNA全长序列，cDNA全长为1 684 bp，基因全长为1 985 bp，含有两个内含子和3个外显子，最大开放阅读框为1 416 bp，预测该基因可编码一个含有471个氨基酸的蛋白质。不计算70 bp的3’末端A／T丰富序列和poly T尾部结构，前人所得到的cDNA拼接序列为1613 bp，最大开放阅读框为1344 bp，预期可编码一个含有447个氨基酸的蛋白质。本文的结果校正了前人cDNA拼接序列及其预测的氨基酸序列的误差。在GeneBank中没有发现与BcMF1同源性较高的功能已知基因，BcMF1仅与拟南芥未知功能蛋白家族DUF1216的相似性较高，是第一个被报导的DUF1216表达基因，蛋白质特征分析显示BcMF1基因编码蛋白很可能是一个定位在细胞外的分泌蛋白。
(2)以两用系的可育株群和不育株群为研究对象，使用Northern杂交的方法较全面地分析了BcMF1的转录表达特征，结果显示BcMF1仅在可育株系的中花蕾、大花蕾、花以及雄蕊中特异表达，在不育株系中不表达。因此，BcMF1是一个与白菜育性相关的新基因。
(3)通过PCR在不育株中扩增了BcMF1序列，通过TAIL PCR在两用系可育株和不育株中同时扩增了该基因的启动子序列，从DNA水平上探讨该基因表达差异形成的分子机理。序列比对发现BcMF1及其启动子序列在可育株和不育株中没有出现变异，表明该基因的这种表达差异不是因为基因或启动子序列突变所引起的，推测BcMF1的表达受其他基因的调控。在BcMF1启动子序列中，发现了一些与光诱导响应、激素响应和胁迫响应有关的顺式作用元件，推测BcMF1的表达可能受到光条件、内外源激素以及逆境胁迫的影响
(4)根据BcMF1的全长序列设计引物，在7种芸薹属蔬菜作物中克隆到BcMF1的同源序列，BcMF1同源基因在核苷酸水平和氨基酸水平相似性分别为97．8％～99．6％和95．3％～98．9％。以同源基因的多序列比对为基础，对7种芸薹属蔬菜作物的BcMF1同源基因进行系统分析，揭示了BcMF1同源基因在这些蔬菜作物中的进化关系。
(5)克隆了菜心BcMF1同源基因并分析其表达特征，证明了菜心适用于白菜BcMF1的功能分析。构建了RNA干涉表达载体pBcMF1-RNAi和反义RNA表达载体pBcMF1-AS，农杆菌介导法转化菜心，获得了Kan抗性菜心植株。分子检测表明已得到了BcMF1-AS转基因植株和BcMF1-RNAi转基因植株，Northern杂交显示BcMF1的表达在转基因植株中受到抑制。对转基因植株的生长状况、花器官发育、花粉形态、花粉离体萌发率以及花粉发育过程进行观察，发现抑制BcMF1的表达可以影响菜心植株的花粉发育，因此推论BcMF1是一个与白菜花粉发育相关的新基因。
(6)根据pBI121质粒多克隆位点两侧的DNA序列设计一对通用引物，以几种pBI121表达载体及其转基因植株为模板进行PCR扩增，并使用限制性内切酶BamHⅠ酶切分析来验证扩增条带的真实性，检测结果与常规鉴定方法完全一致，为各种pBI121表达载体及其转基因植株的鉴定提供了一种快速、有效的方法。
Brassica is one of the most important genus of Cruciferae,and is one of the vegetable crops culturedmost widely in China.Chinese cabbage pak-choi(Brassica campestris L.ssp.chinensis Makino),asubspecies of Brassica campestris L,is a typical allogamy plant with significant heterosis,and thepopularization and application of its F_1 hybrid seeds can improve the level of production in Chinese cabbage.The male sterility lines are very important for producing F_1 hybrid seeds in Chinese cabbage,and thebreeding and utilization of new male sterility lines are paid more attention by researchers.The isolation andfunctional confirmation of male sterility-related genes based on male sterility lines,as well as theinvestigation of behavior characteristics of these genes in chinese cabbage,might be useful to elucidate tothe possible molecular mechanism of plant male sterility,while improving breedings of male sterility lines.
Earlier study in our laboratory has obtaind the preliminary full-length cDNA sequence of a newgene named Brassica campestris Male Fertility 1(BcMF1),by assembling the 5' end,and the 3' end fromRACE with a transcript-derived fragment(TDF) from cDNA-AFLP.In this paper,from the genic malesterile-fertile line‘Bajh97-01AB’of Chinese cabbage taken as a research object,the real full-lengthcDNA and DNA sequences of BcMF1 were cloned by PCR amplification,firstly;the new promotersequences were isolated by TAIL PCR,secondly;the sequence characteristics of BcMF1 and its promoterwere analysed and the function of the hypothetical protein BcMF1 was predicted,thirdly;then RNAinterference(RNAi) expression vector and anti-sense expression vector of BcMF1 were constructed,andtransformed into flowering chinese cabbage(B.campestris ssp.chinensis var.parachinensis Tsen et Lee) byagrobacteriurn-mediated method to obtain their loss-of-function mutants for confirming the function ofBcMF1 in pollen development process;homologous genes of BcMF1 were cloned from deferent species inBrassica by PCR,and the phylogenetic trees of BcMF1 were constructed based on the alignment of thesehomologous genes,lastly.The major study results were as follows:
(1) On the basis of cDNA-AFLP differential fragment BcMF1-A15T17 and its two RACE ends,the real full-length cDNA and DNA sequences of BcMF1 were cloned by PCR.BcMF1 was 1 684-bp long incDNA and 1 985-bp long in DNA,including two introns and three extrons in its DNA sequence,and itslargest open reading frame consisted of 1 416 bp,encoding 471 amino acid residues.The preliminaryfull-length cDNA sequence obtained from earlier study in our laboratory,was 1 613 bp long,notincluding a 70-bp AT-rich sequence and polyT tail,and its largest open reading frame consisted of 1 344 bp,coding 447 amino acid residues.The result in this paper corrected the errors of the cDNA and deducedamino acid sequence of BcMF1 from earlier study.Homology analysis showed that the deduced protein ofBcMF1 with no significant similarity to any known genes,had higher amino acids sequence similarity to aunknown protein family DUF1216 in Arabidopsis thaliana.BcMF1,encoding a extracellular protein inSecretory pathway,is the first expressed gene which had been reported in DUF1216 family.
(2) The expression patterns of BcMF1 in the genic male sterile-fertile line‘Bajh97-01AB’ofChinese cabbage were analyzed by Northern blot.The results showed that BcMF1 specially expressed inmiddle and big flower buds(≥1.0 mm in diameter),open flowers and stamens of the fertile lineBajh97-01B,and didn't express at any stage of the sterility‘Bajh97-01A’line.The results suggest thatBcMF1 is a new gene related with genic male sterility in Chinese cabbage.
(3) The DNA sequence of BcMF1 was amplified by PCR in the sterility line‘Bajh97-01A’,and thepromoter sequences of BcMF1 were isolated simultaneously by TAIL PCR BcMF1 from the fertile line‘Bajh97-01B’and the sterility line‘Bajh97-01A’.The sequence alignment was performed for finding out themolecular cause,in DNA level,why BcMF1 expressed differently between the fertile line‘Bajh97-01B’and the sterility line‘Bajh97-01A’.The results showed that no difference in BcMF1 and its promotersequences was found between the fertile line‘Bajh97-01B’and the sterility line‘Bajh97-01A’,andthe results suggested that the expression difference was not caused by sequence mutantion of BcMF1and its promoter between the fertile line‘Bajh97-01B’and the sterility line‘Bajh97-01A’.Thus,it wasdeduced that another gene or some genes might regulate the expression of BcMF1.In the promotersequence of BcMF1,various cis-elements responding to light inducement,hormone inducement andstress condition were present,so it was predicted that the expression of BcMF1 might be influencedby light,hormone and stress condition.
(4) BcMF1 Homologous genes were isolated from 7 species in Brassica by PCR,and the identification ratio of nucleotide and amino acid sequence were 97.8%～99.6% and 95.3%～98.9% in 7 specises,respectively.The phylogenetic trees were produced on the basis of homologous sequences alignment ofBcMF1 from different seven species in Brassica,and the evolution of homologous BcMF1 in these specieswas demonstrated by the phylogenetic trees.
(5) It was proved that flowering Chinese cabbage is fit to confirm the function of BcMF1,by cloningand expression analysis of the homologous BcMF1 in flowering Chinese cabbage.Anti-sense plantexpression vector pBcMF1-AS and RNAi plant expression vector pBcMF1-RNAi of BcMF1 wereconstructed,and transformed into flowering Chinese cabbage by agrobacterium-mediated method.Themolecular assay showed that pBcMF1-AS and pBcMF1-RNAi constructs were integrated into the genomesof BcMF1-AS and BcMF1-RNAi transgenic plants with Kanamycin resistance respectively,and theexpression level of BcMF1 was down-regulated in BcMF1-AS and BcMF1-RNAi transformants.Growthperformance,floral organ development,pollen morphology,pollen germination in vitro and pollendevelopment of of transgenic plants were observed,these results showed that pollen development wereinfluenced by down-regulated expression of BcMF1 in transgenic plants.These results suggest that BcMF1is a new pollen development-related gene in Chinese cabbage.
(6) Recombinant plant expression vectors constructed on the basis of the the binary vector pBI121,andtheir transgenic plants,were amplified by PCR with a universal primer pair designed according to the flanksequence of multiple clone site in pBI121;Further,the PCR products with predicted sizes were verified bydigestion with the restriction enzyme BamHⅠ.The detecting results by PCR-Digestion were consistent withthese results by the traditional means,therefore a rapid and effective method to identify the expressionvectors and their transgenic plants containing pBI121 plasmid was developed in this paper.
本文以‘矮脚黄’白菜隐性核雄性不育两用系为研究对象，在前人获得雄性不育相关新基因Brassica campestris Male Fertility 1(BcMF1)的cDNA拼接序列的基础上，通过PCR进一步克隆BcMF1的DNA全长和cDNA全长；通过TAIL PCR技术克隆该基因的启动子序列；对BcMF1及其启动子的序列特征以及编码蛋白的功能进行分析和预测；并在几种芸薹属植物中进行BcMF1同源基因的克隆和进化分析。为验证BcMF1的功能，构建了RNA干涉(RNAi)和反义RNA植物表达载体，在分析菜心BcMF1同源基因表达特征的基础上，通过农杆菌介导法转化菜心植株，获取BcMF1表达沉默的卡那霉素抗性植株，对转基因植株的花粉发育状态进行观察，深入分析BcMF1在白菜花粉发育中的生物学功能。本研究获得的主要结果如下：
(1)根据表达差异片段BcMF-A15T17及其RACE扩增序列设计引物，PCR扩增得到BcMF1准确的cDNA全长和DNA全长序列，cDNA全长为1 684 bp，基因全长为1 985 bp，含有两个内含子和3个外显子，最大开放阅读框为1 416 bp，预测该基因可编码一个含有471个氨基酸的蛋白质。不计算70 bp的3’末端A／T丰富序列和poly T尾部结构，前人所得到的cDNA拼接序列为1613 bp，最大开放阅读框为1344 bp，预期可编码一个含有447个氨基酸的蛋白质。本文的结果校正了前人cDNA拼接序列及其预测的氨基酸序列的误差。在GeneBank中没有发现与BcMF1同源性较高的功能已知基因，BcMF1仅与拟南芥未知功能蛋白家族DUF1216的相似性较高，是第一个被报导的DUF1216表达基因，蛋白质特征分析显示BcMF1基因编码蛋白很可能是一个定位在细胞外的分泌蛋白。
(2)以两用系的可育株群和不育株群为研究对象，使用Northern杂交的方法较全面地分析了BcMF1的转录表达特征，结果显示BcMF1仅在可育株系的中花蕾、大花蕾、花以及雄蕊中特异表达，在不育株系中不表达。因此，BcMF1是一个与白菜育性相关的新基因。
(3)通过PCR在不育株中扩增了BcMF1序列，通过TAIL PCR在两用系可育株和不育株中同时扩增了该基因的启动子序列，从DNA水平上探讨该基因表达差异形成的分子机理。序列比对发现BcMF1及其启动子序列在可育株和不育株中没有出现变异，表明该基因的这种表达差异不是因为基因或启动子序列突变所引起的，推测BcMF1的表达受其他基因的调控。在BcMF1启动子序列中，发现了一些与光诱导响应、激素响应和胁迫响应有关的顺式作用元件，推测BcMF1的表达可能受到光条件、内外源激素以及逆境胁迫的影响
(4)根据BcMF1的全长序列设计引物，在7种芸薹属蔬菜作物中克隆到BcMF1的同源序列，BcMF1同源基因在核苷酸水平和氨基酸水平相似性分别为97．8％～99．6％和95．3％～98．9％。以同源基因的多序列比对为基础，对7种芸薹属蔬菜作物的BcMF1同源基因进行系统分析，揭示了BcMF1同源基因在这些蔬菜作物中的进化关系。
(5)克隆了菜心BcMF1同源基因并分析其表达特征，证明了菜心适用于白菜BcMF1的功能分析。构建了RNA干涉表达载体pBcMF1-RNAi和反义RNA表达载体pBcMF1-AS，农杆菌介导法转化菜心，获得了Kan抗性菜心植株。分子检测表明已得到了BcMF1-AS转基因植株和BcMF1-RNAi转基因植株，Northern杂交显示BcMF1的表达在转基因植株中受到抑制。对转基因植株的生长状况、花器官发育、花粉形态、花粉离体萌发率以及花粉发育过程进行观察，发现抑制BcMF1的表达可以影响菜心植株的花粉发育，因此推论BcMF1是一个与白菜花粉发育相关的新基因。
(6)根据pBI121质粒多克隆位点两侧的DNA序列设计一对通用引物，以几种pBI121表达载体及其转基因植株为模板进行PCR扩增，并使用限制性内切酶BamHⅠ酶切分析来验证扩增条带的真实性，检测结果与常规鉴定方法完全一致，为各种pBI121表达载体及其转基因植株的鉴定提供了一种快速、有效的方法。
Brassica is one of the most important genus of Cruciferae,and is one of the vegetable crops culturedmost widely in China.Chinese cabbage pak-choi(Brassica campestris L.ssp.chinensis Makino),asubspecies of Brassica campestris L,is a typical allogamy plant with significant heterosis,and thepopularization and application of its F_1 hybrid seeds can improve the level of production in Chinese cabbage.The male sterility lines are very important for producing F_1 hybrid seeds in Chinese cabbage,and thebreeding and utilization of new male sterility lines are paid more attention by researchers.The isolation andfunctional confirmation of male sterility-related genes based on male sterility lines,as well as theinvestigation of behavior characteristics of these genes in chinese cabbage,might be useful to elucidate tothe possible molecular mechanism of plant male sterility,while improving breedings of male sterility lines.
Earlier study in our laboratory has obtaind the preliminary full-length cDNA sequence of a newgene named Brassica campestris Male Fertility 1(BcMF1),by assembling the 5' end,and the 3' end fromRACE with a transcript-derived fragment(TDF) from cDNA-AFLP.In this paper,from the genic malesterile-fertile line‘Bajh97-01AB’of Chinese cabbage taken as a research object,the real full-lengthcDNA and DNA sequences of BcMF1 were cloned by PCR amplification,firstly;the new promotersequences were isolated by TAIL PCR,secondly;the sequence characteristics of BcMF1 and its promoterwere analysed and the function of the hypothetical protein BcMF1 was predicted,thirdly;then RNAinterference(RNAi) expression vector and anti-sense expression vector of BcMF1 were constructed,andtransformed into flowering chinese cabbage(B.campestris ssp.chinensis var.parachinensis Tsen et Lee) byagrobacteriurn-mediated method to obtain their loss-of-function mutants for confirming the function ofBcMF1 in pollen development process;homologous genes of BcMF1 were cloned from deferent species inBrassica by PCR,and the phylogenetic trees of BcMF1 were constructed based on the alignment of thesehomologous genes,lastly.The major study results were as follows:
(1) On the basis of cDNA-AFLP differential fragment BcMF1-A15T17 and its two RACE ends,the real full-length cDNA and DNA sequences of BcMF1 were cloned by PCR.BcMF1 was 1 684-bp long incDNA and 1 985-bp long in DNA,including two introns and three extrons in its DNA sequence,and itslargest open reading frame consisted of 1 416 bp,encoding 471 amino acid residues.The preliminaryfull-length cDNA sequence obtained from earlier study in our laboratory,was 1 613 bp long,notincluding a 70-bp AT-rich sequence and polyT tail,and its largest open reading frame consisted of 1 344 bp,coding 447 amino acid residues.The result in this paper corrected the errors of the cDNA and deducedamino acid sequence of BcMF1 from earlier study.Homology analysis showed that the deduced protein ofBcMF1 with no significant similarity to any known genes,had higher amino acids sequence similarity to aunknown protein family DUF1216 in Arabidopsis thaliana.BcMF1,encoding a extracellular protein inSecretory pathway,is the first expressed gene which had been reported in DUF1216 family.
(2) The expression patterns of BcMF1 in the genic male sterile-fertile line‘Bajh97-01AB’ofChinese cabbage were analyzed by Northern blot.The results showed that BcMF1 specially expressed inmiddle and big flower buds(≥1.0 mm in diameter),open flowers and stamens of the fertile lineBajh97-01B,and didn't express at any stage of the sterility‘Bajh97-01A’line.The results suggest thatBcMF1 is a new gene related with genic male sterility in Chinese cabbage.
(3) The DNA sequence of BcMF1 was amplified by PCR in the sterility line‘Bajh97-01A’,and thepromoter sequences of BcMF1 were isolated simultaneously by TAIL PCR BcMF1 from the fertile line‘Bajh97-01B’and the sterility line‘Bajh97-01A’.The sequence alignment was performed for finding out themolecular cause,in DNA level,why BcMF1 expressed differently between the fertile line‘Bajh97-01B’and the sterility line‘Bajh97-01A’.The results showed that no difference in BcMF1 and its promotersequences was found between the fertile line‘Bajh97-01B’and the sterility line‘Bajh97-01A’,andthe results suggested that the expression difference was not caused by sequence mutantion of BcMF1and its promoter between the fertile line‘Bajh97-01B’and the sterility line‘Bajh97-01A’.Thus,it wasdeduced that another gene or some genes might regulate the expression of BcMF1.In the promotersequence of BcMF1,various cis-elements responding to light inducement,hormone inducement andstress condition were present,so it was predicted that the expression of BcMF1 might be influencedby light,hormone and stress condition.
(4) BcMF1 Homologous genes were isolated from 7 species in Brassica by PCR,and the identification ratio of nucleotide and amino acid sequence were 97.8%～99.6% and 95.3%～98.9% in 7 specises,respectively.The phylogenetic trees were produced on the basis of homologous sequences alignment ofBcMF1 from different seven species in Brassica,and the evolution of homologous BcMF1 in these specieswas demonstrated by the phylogenetic trees.
(5) It was proved that flowering Chinese cabbage is fit to confirm the function of BcMF1,by cloningand expression analysis of the homologous BcMF1 in flowering Chinese cabbage.Anti-sense plantexpression vector pBcMF1-AS and RNAi plant expression vector pBcMF1-RNAi of BcMF1 wereconstructed,and transformed into flowering Chinese cabbage by agrobacterium-mediated method.Themolecular assay showed that pBcMF1-AS and pBcMF1-RNAi constructs were integrated into the genomesof BcMF1-AS and BcMF1-RNAi transgenic plants with Kanamycin resistance respectively,and theexpression level of BcMF1 was down-regulated in BcMF1-AS and BcMF1-RNAi transformants.Growthperformance,floral organ development,pollen morphology,pollen germination in vitro and pollendevelopment of of transgenic plants were observed,these results showed that pollen development wereinfluenced by down-regulated expression of BcMF1 in transgenic plants.These results suggest that BcMF1is a new pollen development-related gene in Chinese cabbage.
(6) Recombinant plant expression vectors constructed on the basis of the the binary vector pBI121,andtheir transgenic plants,were amplified by PCR with a universal primer pair designed according to the flanksequence of multiple clone site in pBI121;Further,the PCR products with predicted sizes were verified bydigestion with the restriction enzyme BamHⅠ.The detecting results by PCR-Digestion were consistent withthese results by the traditional means,therefore a rapid and effective method to identify the expressionvectors and their transgenic plants containing pBI121 plasmid was developed in this paper.
引文
白林含，刘杨，郑鸣，蒋彦，杨志荣，曹毅．盐藻烯醇酶基因表达载体的构建及其转基因烟草的鉴定．应用与环境生物学报，2003，9 (6)： 603-606．
白新祥，戴思兰．反义RNA技术在花色育种中的应用．植物学通报，2005， 22 (3 )： 284 -291．
本杰明·卢因．基因Ⅷ．北京；科学出版社，2005．
曹家树，曹寿椿，易清明．白菜及其相邻类群基因组DNA的RAPD分析．园艺学报，1995， 22 (1)： 47-52．
曹家树．中国白菜起源、演化和分类研究进展．园艺学年评，北京：科学出版社，1996， 137-160．
曹家树，缪颖，卢钢，曹寿椿．中国白菜各类群的分支分析和演化关系研究．园艺学报，1997，24 (1)： 35-42．
曹家树，叶纨芝，张明，曾广文．白菜核雄性不育两用系花蕾的mRNA差别显示及其cDNA差异片段分析．浙江大学学报(农业与生命科学版)， 2001， 27 (6)： 596-600．
曹家树，余小林，叶纨芝，向珣，卢钢，王永勤，黄鹂，黄科，王玲平，张弢．白菜核雄性不育相关基因的研究．中国园艺文摘，2005， 21 (5)： 39-44．
曹寿椿，李式军．矮脚黄白菜雄性不育系两用系的选育与利用．南京农学院学报，1980(1)：59-67．
曹寿椿，李式军．火白菜“矮杂一号”及雄性不育两用系的选育．园艺学报，1981，8(3)：35-41．
曹寿椿，侯喜林，张蜀宁，朱月林，刘克均．不结球白菜抗病育种的研究Ⅶ．矮抗四号新品种的选育．南京农业大学学报，1998，21 (2)： 24-29．
陈云鹏，曹家树，缪颖，叶纨芝．芸薹类蔬菜DNA遗传多样性RAPD分析．浙江大学学报(农业与生命科学版)，2000， 26 (2)： 131-136．
杜娟，朱祯，李晚忱．植物逆境诱导启动子mwcsl20的克隆及表达特性研究．作物学报，2005，31 (10)： 1328-1332．
盖树鹏，孟祥栋．转基因植物的筛选与检测．山东农业大学学报[自然科学版]， 2000，31 (1)： 95-100．
顾雪松，陈章良、朱玉贤．光敏色素与光调控．植物学报(英文版)，1997，39 (7)： 675-681．
郭晶心，周乃元，马容才，曹鸣庆．白菜类蔬菜遗传多样性的AFLP分子标记研究．农业生物技术学报，2002， 10 (2)： 138-143．
韩建明，侯喜林，徐海明，史公军，耿建峰，邓晓辉．不结球白菜(Brassica campestris ssp．chinensis Makino)种质资源SRAP遗传分化分析．作物学报，2007， 33(11)：1862-1868．
何余堂，陈宝元，傅廷栋，李殿荣，涂金星．白菜型油菜在中国的起源与进化．遗传学报，2001，28 (4)： 331-335．
候爱菊，朱延明，张晶，李杰，张彬彬．转基因植物中筛选标记基因的利用及消除．遗传，2003，25 (4)： 466-470．
胡维，向华．用PCR法直接快速筛查重组阳性克隆．中国微生态学杂志，2000， 12 (1) ： 1-2
黄鹂，曹家树，张强．十字花科植物花粉发育相关基因的研究Ⅰ．自然科学进展，2005a， 15 (8)： 907-916．
黄鹂，曹家树．十字花科植物授粉过程中花粉表达基因的研究Ⅱ．自然科学进展，2005b， 15 (11)： 1287-1296．
黄鹂，曹家树，叶意群，张豫超．甘蓝花粉特异表达的多聚半乳糖醛酸酶基因BoMF9的克隆与特征分析．农业生物技术学报，2007a， 15 (2)： 268-273．
黄鹂，曹家树，张豫超，叶意群．一个白菜花粉发育相关的新基因BcMF7的鉴定．中国科学C， 2007b， 37 (4)：479-487．
黄鹂．白菜三种雄性不育系与保持系花蕾转录组差异分析及三个花粉发育相关基因功能鉴定浙江大学博士论文，2007c．
金松恒，翁晓燕，王妮妍，李雪芹，毛伟华，蒋德安．Rubisco活化酶基因反义表达载体的构建与水稻的遗传转化．遗传，2004， 26 (6)： 881-886．
康俊根，王晓武，张国裕，张延国，娄平，方智远．利用cDNA-AFLP检测甘蓝雄性不育相关基因的时序性表达．园艺学报，2006， 33 (3)： 544-548．
孔凡江，吕慧颖，杨庆凯，赵奎军，陈庆山，宁海龙．植物转基因中应用的筛选体系．东北农业大学学报，2002， 33(2)：191-197．
廖世奇，张春梅，张雪力，孙雨葳，王黎，王晓辉．一种PCR快速鉴定重组体DNA阳性克隆及插入方向的方法．科学技术与工程，2004， 4 (02)： 89-90， 96．
李德葆，周雪平，许建平，何祖华．基因工程操作技术．上海：上海科学技术出版社，1996．
李晶，李杰，关英芝，朱延明．逆境诱导型启动子rd29A的克隆及植物表达载体的构建．植物研究，2004， 24 (1)：111-114．
刘会超，李永春，巨关生，孙振元，柴德勇．香石竹ACC氧化酶基因克隆及其反义表达载体构建．核农学报，2005， 19(6)：461-464，473．
刘慧丽，李玲．脱落酸(ABA)诱导基因表达的调控元件植物学通报，2001， 18 (3)： 276-282．
刘乐承，向珣，曹家树．白菜雄性不育相关基因BcMF4基因功能的RNAi验证．遗传，2006， 28(11)： 1428-1434．
刘乐承，向珣，曹家树．RNAi沉默BcMF3基因对菜薹花粉发育的影响．园艺学报，2007， 34 (1 )： 125-130．
娄平，王晓武，Guusje Bonnema，方智远．利用cDNA-AFLP技术克隆鉴定甘蓝显性核不育相关基因表达序列园艺学报，2003， 30 (6)： 668-672．
卢钢，曹家树陈杭．芸薹属植物分子标记技术和基因组研究进展．园艺学报1999．26(6 )，384 -390
路静，赵华燕，何奕昆，宋艳茹高等植物启动子及其应用研究进展．自然科学进展，2004， 14 (8)： 856-862．
那杰，王关林，夏然，杨怀义．草莓annfaf基因反义融合表达载体构建及转基因植株的获得．中国农业科学，2006， 39(3)：582-586．
萨姆布鲁克J，拉赛尔D．W．分子克隆实验指南，第3版．北京：科学出版社，2002．
邵宏波，梁宗锁，邵明安．高等植物对环境胁迫的适应与其胁迫信号的转导．生态学报，2005， 25 (7)： 1772-1781．
宋江华．白菜花粉发育相关基因BcMF11和BcMF12的克隆、表达及其功能验证。浙江大学博士学位论文，2006．
帅琪峰，叶纨芝，金维正，曹家树，张明，甘德芳．白菜细胞核雄性不育两用系的性状比较分析，上海农业科学，2004，20 (3)：48-51．
孙德岭，赵前程，宋文芹，陈瑞阳．白菜类蔬菜亲缘关系的AFLP分析．园艺学报，2001， 28 (4)： 331-335．
孙日飞，吴飞燕，司家钢．大白菜核雄性不育两用系小孢子发生的细胞形态学研究．园艺学报，1995，22： 153-156．
孙日飞，方智远，张淑江．萝卜胞质大白菜雄性不育系的生化分析．园艺学报， 2000， 27 (2)： 187-192．
涂红艳，刘元风．反义RNA的作用机理及其在植物基因工程领域的应用．生物磁学．2005，5 (2)： 32-34．
王关林，方宏筠．植物基因工程，第2板北京：科学出版社，2002．
王关林，孙月剑，那杰，李洪艳，方宏筠．中国转基因植物产业化的研究进展及存在问题．中国农业科学，2006， 39 (7)：1328-1335．
王玲平，曹家树，叶纨芝，向珣，周生茂十字花科植物CYP86MF基因同源序列的克隆与进化分析．遗传，2005， 27(3)：385-402．
王铃平．十字花科植物CYP86MF同源基因的结构、功能及进化关系的研究．浙江大学博士学位论文，2004．
王钦丽，卢龙斗，吴小琴，陈祖铿，林金星．花粉的保存及其生活力测定．植物学通报，2002， 29 (3)： 365-373
王亚馥，胡昌勤，林志刚等．大白菜雄性不育两用系可育株不育株的比较分析．园艺学报，1984，11 (3)： 182-186．
王永勤，曹家树，虞慧芳，叶纨芝，余小林，向珣，卢钢．白菜核雄性不育两用系生理生化特性的分析，园艺学报，2003 a，30 (2)： 212-214．
王永勤，曹家树，付庆功，余小林，叶纨芝，向珣．利用cDNA-AFLP技术分析白菜核雄性不育两用系的表达差异．中国农业科学，2003b， 36 (5)： 557-560
王永勤．白菜核不育两用系育性基因表达特征分析及其小孢子发育相关基因的分离．浙江大学博士学位论文，2003c．
王永勤，余小林，曹家树．白菜小孢子发育相关基因BcMF3的分离及序列分析．遗传学报，2004， 31 (11 )：1302-1308．
吴建勇，沈俊儒，刘平武，杨光圣．应用cDNA-AFLP研究甘蓝型油菜显性细胞核雄性不育差异表达基因．中国农业科学，2006， 39 (9)： 1921-1926．
向旭，傅家瑞．脱落酸应答基因的表达调控及其与逆境胁迫的关系．植物学通报，1998， 15 (3)： 11-16．
向珣，曹家树，叶纨芝，崔辉梅，俞建浓．白菜OguCMS相关MYB家族新基因BcMYB的克隆与特征分析．遗传，2007，29 (5)：621-628
肖玲，卢长明．以油菜脂肪酸延长酶基因fael为靶标RNAi载体的构建．中国油料作物学报，2004， 26(4) ： 6-11．
萧灿，金振华．反义基因操作及反义分子的设计原则生物工程进展．1993， 14 (2)： 27-29
许德晖，黄辰，刘利英，宋土生．高效siRNA设计的研究进展．遗传，2006， 28 (11)： 1457-1461．
燕飞，成卓敏．RNA干扰机制研究进展．遗传，2005， 27(1)： 167-172．
杨小二，孔华，郭安平，贺立卡．RNAi技术及其在植物分子生物学中的应用．分子植物育种，2005，3 (4)： 571-74．
叶纨芝，曹家树．植物雄性不育的分子机理．植物生理学通讯，2000，36 (2)： 176-181
叶纨芝．白菜细胞核雄性不育相关基因mf-CYP450的分子鉴定、克隆和序列分析．浙江大学博士学位论文，2001．
叶纨芝，曹家树，余小林，崔辉梅，黄鹂，向珣，陶甦．白菜细胞核雄性不育两用系的细胞学观察．细胞生物学杂志，2004，26 (5)：516-522．
叶意群．白菜多聚半乳糖醛酸酶基因BcMF9的功能分析．浙江大学硕士学位论文，2006．
余小林，曹家树，徐淑英．改良菜心离体培养植株再生体系的研究．实验生物学报，2001， 34 (2)： 157-161．
余小林白菜雄性不育相关基因CYP86MF的功能验证及其人公布于细的创建．浙江大学博士学论文，2002．
余小林，曹家树，许立奎，齐文雯，王小静．优化白菜类蔬菜遗传转化体系的研究．浙江大学学报(农业与生命科学版)，2005， 31 (5)： 529-534．
袁定阳，段美娟，谭炎宁，易自力，袁隆平，辛世文．共转化法获得无筛选标记的转PEPC， PPDK基因水稻恢复系纯合体．杂交水稻(HYBRID RICE) 2007，22 (2)： 57-63．
张春晓，王文棋，蒋湘宁，陈雪梅．植物基因启动子研究进展．遗传学报，2004， 31 (12)： 1455-1464．
张国裕，康俊根，张延国，程智慧，王晓武．青花菜雄性不育相关基因BoDHAR的克隆与表达分析．生物工程学报，2006a，22 (5) ： 751-756．
张国裕，康俊根，张延国，娄平，程智慧，王晓武．青花菜快速碱化因子RALF的克隆与序列分析，园艺学报，2006b， 33(3)：561-565．
张明．白菜核雄性不育基因的分子标记及遗传分析．浙江大学硕士学位论文，2000．
张强，黄鹂，曹家树．白菜多聚半乳糖醛酸酶基因BcMF6的克隆、序列分析及其表达．园艺学报，2007， 34 (1)： 117-124．
张玉，许向阳，李景富．反义RNA技术调控番茄果实耐贮性研究进展分子植物育种，2003， 1 (5/6)： 707-711．
张豫超．白菜雄性不育相关基因BcMF10的功能验证．浙江大学硕士学位论文，2006．
张豫超，叶意群，黄鹂，曹家树花粉发育相关基因BjMF6的分子克隆及其生物信息学分析．细胞生物学杂志，2007， 29(1)：158-162．
张弢．十字花科植物BcMF2和BcMF4同源基因的克隆，表达及其进化关系研究．浙江大学博士论文，2005．
赵庆臻，赵双宜，夏光敏．植物RNA沉默机制的研究进展．遗传学报，2005 ， 32 (1)：104-110．
曾雅明，何艺宾，张红心，陈亮．OsICK1基因植物反义表达载体的构建及遗传转化．厦门大学学报(自然科学版)，2006， 45 (B05)：66-69
宗建超，倪爱国，刘福森．反义技术研究进展．生物化学与生物物理进展，1992， 19 (1)： 10-14．
周长久，王鸣，吴定华，李景富．现代蔬菜育种学北京：科学技术文献出版社，1996．
朱龙付，张献龙．RNA i及其在植物遗传改良中的应用华中农业大学学报，2004 ， 23 (3) ： 472-477．
Altschul S F， Madden T L， Schaffer A A， Zhang J H， Zhang Z， Miller W and Lipman D J．Gapped BLAST and PSI-RLAST： A new generation ofprotein database search programs Nucleic Acids Res199725： 3389-3402．
Ahmed K， Davis A T， Wang H， Faust R A， Yu S， Tawfic S．Significance of protein kinase CK2 nuclear signaling in neoplasia．J Cell Biochem Suppl， 2000， 35： 130-135．
Asano T，Katagiri H， Takata K， Lin J L， Ishihara H， Inukai K， Tsukuda K， Kikuchi M．The role of N-glycosylation of GLUT1 for glucose transport activity．J Biol Chem， 1991，266： 24632-24636．
Bachem C W B， van der Hoeven R S， de Bruijn S M， Vreugdenhil D， Zabeau M， Visser R G F．Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP： analysis of gene expression during potato tuber development．Plant J， 1996， 9： 745-753．
Becker D， Kemper E， Schell J， Masterson R．New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol Biol, 1992, 20: 1195 -1197.
Bcvan M W. Binary Agrobactcrium vectors for plant transformation. Nucleic Acids Res, 1984, 12: 1811-1821.
Block A, Dangl J L, Hahlbrock K, Schulze-Lefcrt P. Functional borders, genetic fine structure, and distance requirements of cis elements mediating light responsiveness of the parsley chalcone synthase promoter. PNAS, 1990, 87: 5387-5391.
Boisson B, Giglione C, Meinnel T. Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote. J Biol Chem, 2003, 278: 43418-43429.
Borgese N, Aggujaro D, Carrera P, Pietrini G, Bassetti M. A Role for N-myristoylation in protein targeting: NADH-cytochrome b5 reductase requires myristic acid for association with outer mitochondrial but not ER membranes. J Cell Biol, 1996, 135: 1501-1513.
Bracha-Drori K, Shichrur K, Katz A, Oliva M, Angelovici R, Yalovsky S, Ohad N. Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. Plant J, 2004, 40: 419-427.
Burch-Smith T M, Schiff M, Liu Y, Dinesh-Kumar SP. Efficient virus-induced gene silencing in Arabidopsis. Plant Physiol, 2006, 142: 21-27.
Buteau H, Pezet A, Ferrag F, Perrot-Applanat M, Kelly PA, Edery M. N-glycosylation of the prolactin receptor is not required for activation of gene transcription but is crucial for its cell surface targeting. Mol Endocrinol, 1998, 12: 544-555.
Cao J S, Yu X L, Ye W Z, Lu G, Xiang X. Functional analysis of a novel male fertility CYP86MF gene in Chinese cabbage (Brassica campestris L. ssp. chinensis Makino). Plant Cell Rep, 2006, 24(12): 715-723.
Chaman A K, Alan C, Hiroshi A. Inhibition of caffeine biosynthesis in tea (Camellia sinensis) and coffee (Coffa arabica) plants by ribavirin. FEBS Letters, 2003, 554: 473-477.
Chakrabarty R, Banerjee R, Chung S M, Farman M, Citovsky V, Hogenhout S A, Tzfira T, Goodin MM. pSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-vhus interactions. Mol Plant Microbe Interact, 2007, 20: 740-750.
Chen P Y, Wang C K, Soong S C, To KY. Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Mol Breeding, 2003, 11: 287-293.
Chuang C F, Meyerowitz E M. Specific and heritable genetic interference by double -stranded RNA in Arabidopsis thaliana. PNAS,2000,97(9):4985-4999.
Citovsky V, Lee LY, Vyas S, Glick E, Chen M H, Vainstein A, Gafni Y, Gelvin SB, Tzfira T (2006) Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planla. J Mol Biol, 362: 1120-1131.
Clement S A, Tan C C, Guo J. Roles of protein kinase C and alpha-tocopherol in regulation of signal transduction for GATA-4 phosphorylarion in HL-1 cardiac muscle cells. Free Radic Biol Med, 2002, 32: 341-349.
Coutu C, Brandle J, Brown D, Brown K, Miki B, Simmonds J, Hegedus D D. pORE: a modular binary vector series suited for both monocot and dicot plant transformation. Transgenic Res, 2007, 16: 771-781.
Curtis M D, Grossniklaus U. A Gateway cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol, 2003, 133:462-469.
Dafny-Yelin M, Tzfira T. Delivery of multiple transgenes to plant cells. Plant Physiol, 2007; 145: 1118-1128.
Datla R S, Hammerlindl J K, Panchuk B, Pelcher L E, Keller W. Modified binary plani transformation vectors with the wild-type gene encoding NPTI1. Gene, 1992, 122: 383-384.
de Buck S, Peck I, de Wilde C, Marjanac G, Nolf J, de Paepe A, Depicker A. Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system. Plant Physiol, 2007, 145: 1171-1182.
de Block, Debrouwer D. Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly intergrated at the ame locus. Theor Appl Genet, 1991, 82: 257-263.
Daley M, Knauf V C, Summerfelt K R. Co-transformtion with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep, 1998, 17: 489-496.
Ding D X H, Vera J C, Heaney M L, Golde D W. N-glycosylation of the human granulocyte-macrophage colony-stimulating factor receptor alpha subunit is essential for ligand binding and signal transduction. J Biol Chem, 1995, 270: 24580-24584.
Ebinuma H, Sugita K, Matsunaga E, Yamakado M. Selection of marker-free transgenic plants using the isopentenyl transferase gene. PNAS, 1997, 94: 2117-2121.
Ebinuma H. Sugita K, Matsunaga E, Endo S, Yamada K, Komamine A. System for the removal of a selection marker and their combination with Positive marker. Plant Cell ReP, 2001, 20: 383-392.
Escobar M A, Civerolo E L, Summerfelt K R, Dandekar A M. RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis. PNAS, 2001, 98: 13437-13442.
Fernandez B, Czech M P, Meisner H. Role of protein kinase C in signal attenuation following T cell receptor engagement. J Biol Chem, 1999, 274: 20244-20250.
Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins M R, Appel R D, Bairoch A. Protein identification and analysis tools on the ExPASy Server. (In) John M. Walker (ed): The Proteomics Protocols Handbook, 2005: 571-607.
Gilbert C, Gaudry M, Naccache P H. Rapid priming of calcium mobilization and superoxide anion production in human neutrophils by substimulatory concentrations of phorbol esters: a novel role for protein kinase C and tyrosine phosphorylation in the up-modulation of signal transduction. Cell Signal, 1992, 4: 511-523.
Gleba Y, Klimyuk V, Marillonnet S. Magnifection - a new platform for expressing recombinant vaccines in plants. Vaccine, 2005, 23- 2042-2048
Goodin M M, Chakrabarty R, Banerjee R, Yelton S, DeBolt S. New gateways to discovery. Plant Physiol, 2005, 145: 1100-1109.
Grobe K, Powell L D. Role of protein kinase C in the phosphorylation of CD33 (Siglec-3) and its effect on lectin activity. Blood, 2002, 99:3188-3196.
Guo H S, Fei J F, Xie Q, Chua N H. A chemical-regulated inducible RNAi system in plants. Plant J, 2003: 34(3):383-392.
Hajdukiewicz P, Svab Z, Maliga P. The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol, 1994, 25: 989-994.
Hartley J L, Temple G F, Brasch M A. DNA cloning using in vitro site-specific recombination. Genome Res, 2000, 10: 1788-1795.
Haldrup A, Petersen S G, Okkels F T. Positive selection: a plant selection principle based on xylose isomerase, an enzyme used in the food industry. Plant Cell Rep, 1998, 18:76-81.
Higo K, Ugawa Y, Iwamoto M, Korenaga T. Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Research, 1999, 27(1): 297-300.
Hofmann K, Stoffel W. TMbase - A database of membrane spanning proteins segments. Biol.Chem Hoppe-Seyler, 1993, 374, 166.
Hoekema A, Hirsch P R, Hooykaas P J J, Schilperoort R A. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature, 1983, 303: 179-180.
Huang L, Cao J S, Zhang Y C, Ye Y Q. Characterization of a novel gene, BcMF7 that is expressed preferentially in pollen of Brassica campestris L. ssp. chinensis Makino, Sci China C Life Sci, 2007a, 50(4): 497-504.
Huang L, Cao J S, Zhang A H, Ye Y Q. Characterization of a putative pollen-specific arabinogalactan protein gene, BcMF8, from Brassica campestris ssp. chinensis. Mol Biol Rep, 2007b, Sep 5.
Huang L, Cao J S, Ye W Z, Liu T T, Jiang L X, Ye Y Q. Transcriptional differences between the male sterile mutant bcms and wild type of Brassica campestris ssp. chinensis reveal genes related to pollen development. Plant Biol, 2008, 10 (3): 342-355.
Jannick D B, Henrik N, Gunnar V H, Soren B. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol, 2004, 340:783-795.
Jeanmougin F, Thompson J D, Gouy M, Higgins D G, Gibson T J. Multiple sequence alignment with Clustal X. Trends Biochem Sci, 1998, 23,403-405.
Jefferson R A. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep, 1987a, 5: 387-405 Jefferson R A, Kavanagh T A, Bevan M W. GUS fusions: P-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J, 1987b, 6 (13): 3901-3907.
Joersbo M, Okkels F T. A novel principle for selection of positive seletion of transgenic plant cells. Plant Cell Rep, 1996, 16: 219-221.
Joersbo M, Donaldson I, Kreiberg J, Petersen S G, Brunstedr (?) Okkels F T. Analysis of mannose selection used for transformation of sugar beet. Molecular Breeding, 1998,4: 111-117.
Jones J D G, Carland F, Lim E, Ralston E, Dooner H K. Preferential transposition of the maize element activator to linked chromosomal loctions in tobacco. Plant Cell, 1990, 2: 701-707.
Kai G Y, Zhang L, Zhang H Y, Xu T F, Tang K X, Zhang H M. Marker-free: a novel tendency of transgenic plants. Acta Botanica Sinica , 2002, 44: 883-888.
Kaneko T, Katoh T, Sato S, Nakamura Y, Asamizu E, Tabata S. Structural analysis of Arabidopsis thaliana chromosome 3. @@@. Sequence features of the 4251695 bp regions covered by 90 PI, TAC and BAC clones. DNA Res. 2000, 7: 217-221.
Karimi M, Bleys A, Vanderhaeghen R, Hilson P. Building blocks for plant gene assembly. Plant Physiol, 2007, 145: 1183-1191
Kawakami Y, Kitaura J, Hartman S E, Lowell C A, Siraganian R P, Kawakami T. Regulation of protein kinase C@@@ by two protein-tyrosine kinases, Btk and Syk. PNAS, 2000, 97: 7423-7428.
Kobe F, Yvan S, Svcn D, Pierre R, Yves V P. Large-scale structural analysis of the core promoter in mammalian and plant genomes. Nucleic Acids Research, 2005, 33: 4255-4264.
Komari T, Takakura Y, Ueki J, Kato N, Ishida Y, Hiei Y. Binary vectors and super-binary vectors. Methods Mol Biol, 2006, 343: 15-42.
Komori T, Imayama T, Kato N, Ishida Y, Ueki J, Komari T. Current status of binary vectors and superbinary vectors. Plant Physiol, 2007, 145: 1155-1160.
Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T. Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J, 1996, 10:165-174.
Kumar S, Tamura K, Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics, 2004, 5: 150-163.
Lam E, Chua N H. ASF-2: A Factor That Binds to the Cauliflower Mosaic Virus 35S Promoter and a Conserved GATA Motif in Cab Promoters. Plant Cell, 1989, 1: 1147-1156.
Lassmann T, Sonnhammer E L L. Automatic assessment of alignment quality. Nucleic Acids Res, 2005, 33: 7120-7128.
Lee L Y, Kononov M E, Bassuner B, Frame B R, Wang K, Gelvin S B. Novel plant transformation vectors containing the superpromoter. Plant Physiol, 2007, 145: 1294 -1300.
Li L B, Chen N, Ramamoorthy S, Chi L, Cui X N, Wang L C, Reith M E A. The Role of N-glycosylation in function and surface trafficking of the human dopamine transporter. J Biol Chem, 2004, 279: 21012-21020.
Liu L C, Cao J S, Yu X L, Xiang X, Pei Y J. Expression of an antisense BcMF3 affects microsporogenesis and pollen tube growth in Arabidopsis. Agricultural Sciences in China, 2006, 5(5): 339-345.
Liu L C, Wang Y Q, ZHang T, Huang L, Xiang X, Cao J S. Isolation and characterization of the microspore-related gene BcMF4 in Chinese cabbage-pak-choi and its functional identification in Arabidopsis. J Horticultural Sci Biotechnol, 2007, 82(1): 133-139.
Li Q L,Yin H,Li D,Zhn H F,Zhang Y,Zhu W Wi,Isolation and Characterization of GMO Gene Promoter from Halophyte Suaeda liaotungensis K. J Genet Genomics, 2007, 34: 355-361.
Li Y Y, Cao J S, Huang L, Yu X L, Xiang X. BcMF13, a new reproductive organ-specific gene from Brassica campestris ssp. chinensis, affects pollen development. Mol Biol Rep, Published online: 27, March, 2007.
Lin R, Hiscott J. A role for casein kinase @@@ phosphorylation in the regulation of IRF-1 transcriptional activity. Mol Cell Biochem, 1999, 191: 169-180.
Liu Y G, Whittier R F. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics, 1995, 25: 674 -681.
Lizbeth H, Lu G IMP dehydrogenase: structural schizophrenia and an unusual base. Current Opinion in Chemical Biology, 2006,10:520-525.
Louwerse J D, van Lier M C, van der Steen D M, de Vlaam C M, Hooykaas P J, Vergunst A C. Stable recombinase-mediated cassette exchange in Arabidopsis using Agrobacterium tumefaciens. Plant Physiol, 2007, 145: 1282-1293.
Lucca P, Ye X D, Potrykus I. Effective selection and regeneration of transgenic rice plants with mannose as selective agent. Molecular Breeding, 2001, 7: 43-49.
Lutcke H A, Chow K C, Mickel F S, Moss K A, Kern H F, Scheele G A. Selection of AUG initiation codons differs in plants and animals. EMBO J, 1987, 6: 43-48.
Lutz K A, Azhagiri A K, Tungsuchat-Huang T, Maliga P. A guide to choosing vectors for transformation of the plastid genome of higher plants. Plant Physiol, 2007, 145: 1201-1210.
Magali Lescot, Patrice DChais, Gert Thijs, Kathleen Marchal, Yves Moreau, Yves Van de Peer, Pierre Rouz and Stephane Rombauts. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res. 2002, 30(1): 325-327.
Marco Pagni, Vassilios Ioannidis, Lorenzo Cerutti, Monique Zahn-Zabal, C. Victor Jongeneel, Jorg Hau, Olivier Martin, Dmitri Kuznetsov and Laurent Falquet. MyHits: improvements to an interactive resource for analyzing protein sequences. Nucleic Acids Research, 2007, 35, suppl_2 W433-W437.
Marillonnet S, Thoeringer C, Kandzia R, Klimyuk V, Gleba Y. Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat Biotechnol, 2005, 23: 718-723.
Matthews P R, Wang M B, Waterhouse P M, Thornton S, Fieg S J, Gubler F, Jacobsen J V. Marker gene elimination from transgenic barley, using co-transformation with adjacent 'twin T-DNAs' on a standard agrobacterium transformation vector. Molecular Breeding, 2001, 7: 195-202.
McKnight S L, Kingsbury R. Transcriptional control signals of a eukaryotic protein-coding gene. Science, 1982, 217: 316-324.
Meyer S, Nowak K, Sharma V K, Schulze J, Mendel R R, Hansch R. Vectors for RNAi technology in poplar. Plant Biol, 2004, 6: 100-103.
Negrotto D, Jolley M, Beer S, Wenck A R, Hansen G The use of phosphomannose-isomerase as a selectable marker to recover transgenic maize plants(Zea mays L.) via Agrobacterium transformation. Plant Cell Rep, 2000, 19: 798-803.
Niu L, Hcancy M L, Vcra J C, Golde D W. High-affinity binding to the GM-CSF receptor requires intact N-glycosylatinn sites in the extracellular domain of the beta subunit. Blood, 2000, 95: 3357-3362.
Emanuelsson O, Nielsen H, Brunak S, von Heijne G Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol, 2000, 300: 1005-1016.
Ohad N, Shichrur K, Yalovsky S. The analysis of protein-protein interactions in plants by bimolecular fluorescence complementation. Plant Physiol, 2007, 145: 1090-1099.
Onouchi H, Nishihama R, Kudo M. Visualization of site-specific recombination catalyzed by a recombinase from Zygosacchacomyces rouxii in arabidopsis thaliana. Mol Gen Genet, 1995, 247: 653-660.
Park J, Hill M M, Hess D, Brazil D P, Hofsteenge J, Hemmings B A. Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 and their role in regulating kinase activity. J Biol Chem, 2001, 276: 37459-37471.
Morton P, Park K. J, Obayashi T, Fujila N, Harada H, Adams-Collier C J, Nakai K. WoLF PSORT: Protein Localizalion Predictor. Nucleic Acids Research, 2007, 1-3, doi:10.1093/nar/gkm259, 2007.
Stoutjesdijk P A, Singh S P, Liu Q, Hurlstone C J, Waterhouse P A, Green A G hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing . Plant Physio), 2002, 129(4): 1723-1731.
Prestridge, D S. SIGNAL SCAN: A computer program that scans DNA sequences for eukaryotic transcriptional elements. Compul Applic Biosci, 1991,7: 203-206.
Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, Lopez R InterProScan: protein domains identifier. Nucleic Acids Res, 2005, 33: 116-120.
Gupta R, Ting JTL, Sokolov L N, Johnson S A, Luan S. A Tumor suppressor homolog, AtPTEN1, is essential for pollen development in arabidopsis. Plant Cell, 2002,14: 2495-2507.
Reed J, Privallel M, Powell M L, Meghji M, Dawson J, Dunder E, Sutthe J, Wenck A, Launis K, Kramer C, Chang Y F, Hansen G, Wright M. Phosphomannose isomerase: an efficient selectable marker for plant transformation. In Vitro Cell Dev Boil-Plant, 2001, 37: 127-132.
Robertson M. Gene Regulation: specificity and flexibility. Nature, 1987, 327: 464-466.
Rothstein S J, DiMaio J, Strand M, Rice D. Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA. PNAS, 1987, 84: 8439-8443.
Salinas J, Oeda K, Chua N-H. Two G-Box-Related Sequences Confer Different Expression Patterns in Transgenic Tobacco.Plant Cell, 1992,4: 1485-1493.
Samalova M, Brzobohaty B, Moore I. pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco. Plant J, 2005, 41: 919-935.
Sato S, Nakamura Y, Kaneko T, Katoh T, Asamizu E, Tabata S. Structural analysis of Arabidopsis thaliana chromosome 3.1. Sequence features of the regions of 4, 504, 864 bp covered by sixty PI and TAC clones. DNA Res. 2000, 7: 131-135.
Sa to S, Kaneko T, Kotani H, Nakamura Y, Asamizu E. Structural analysis of Arabidopsis thaliana chromosome 5. @@@ Sequence features of the regions of 1,456.315 bp covered by nineteen physically assigned PI and TAC clones. DNA Res 1998,5:41-54.
Shahmuradov I A, Gammerman A J, Hancock J M, Bramley P M, Solovyev V V. PlantProm: a database of plant promoter sequences. Nucleic Acids Res, 2003, 31(1): 114-117.
Smithe C J S, Watson C f, Ray J, Bird C R, Morris P C, Schuch W, Grierson D. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature, 1988, 334: 724 -726.
Solovyev V V, Shahmuradov I A. PromH: Promoters identification using orthologous genomic sequences. Nucleic Acids Res, 2003,31:3540-3545.
Stephane R, Patrice D, Marc V M, Pierre R. PlantCARE, a plant cis-acting regulatory element database. Nucleic Acids Res. 1999, 27(1): 295-6.
Spitzer B, Ben Zvi MM, Ovadis M, Marhevka E, Barkai O, Edelbaum O, Marton I, Masci T, Alon M, Morin S. Reverse genetics of floral scent: application of tobacco rattle virus-based gene silencing in petunia. Plant Physiol, 2007, 145: 1241-1250.
Sugita K, Kasaharat T, Matsunagae E,. A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J, 2000. 22: 461-469
Tada Y, Sakamoto M, Matsuoka M, Fujimura T. Expression of a monocot LHCP promoter in transgenic rice. EMBO J, 1991, 10: 1803-1808.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. Nature, 2000, 408: 823-826.
Thompson, J D, Gibson,T J, Plewniak, F, Jeanmougin, F and Higgins DG The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 1997, 24: 4876-4882.
Todd R, Tague B W. Phosphomannose isomerase: A versatile selectable marker for arobidopsis thaliana germ-line transformation. Plant Molecular Biology Reporter, 2001, 19:307-319.
Ts uchida M, Manthei E R, Alam T, Knechtle S J, Hamamy M M. Regulation of T cell receptor and CD28-induced tyrosine phosphorylation of the focal adhesion tyrosine kinases Pyk2 and Fak by protein kinase C. J Biol Chem, 2000, 275: 1344-1350.
Twell D. Pollen developmental biology. In: O'Neill S D, Roberts J A, eds, Plant Reproduction. Annual Plant Reviews, Sheffield: Sheffield Academic Press, 2002, 6: 86-153.
Tzfira T, Tian G W, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, Krichevsky A, Taylor T, Vainstein A, Citovsky V. pSAT vectors: a modular series of plasmids for fluorescent protein tagging and expression of multiple genes in plants. Plant Mol Biol, 2005,57: 503-516.
Voinnet O, Rivas S, Mestre P, Baulcombe D C. An enhanced transient expression system in plants based on suppression of gene silencing by the pl9 protein of tomato bushy stunt virus. Plant J, 2003, 33: 949-956.
Walter M, Chaban C, Schtze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C. Visualization of protein interactions in living plant cells usine bimolecular fluorescence complementation. Plant J, 2004, 40: 428-438.
Wang E M, Wagner G J. Elucidation of the functions of genes central to diterpene metabolism in tobacco trichomes using posttranscriptional gene silencing. Planta, 2003, 216: 686-691.
Wang Y Q, Cao J S, Fu Q G, Yu X L, Ye W Z, Xiang X. Differential expression analysis of genie male sterility A/B lines in Chinese cabbage -pak-choi(Brassica campestris ssp. chinensis Makino). Agricultural Sciences in China, 2003, 2: 195-199.
Wang Y Q, Ye W Z, Cao J S, Yu X L, Xiang X, Lu G Cloning and characterization of the microspore development-related gene BcMF2 in Chinese cabbage pak-choi (Brassica campestris L. ssp. chinensis Makino). Journal of Integrative Plant Biology．2005， 47(7)： 863-872．
Wang N J， Cheng Q， Ren Y G， Zhang L J， Shen G F．Studies on tissue-specific expression direction by rice 4-coumarate CoA ligase(4CL) promoter．Science Agricultura Sinica， 1996，29： 73-77．
Wesley S V， Helliwell C A， Smith N A， Wang M G B， Rouse D T， Liu Q， Gooding P S， Singh S P， Abbott D， Stoutjesdijk P A．Robinson S P， Gleave A P， Green A G， Waterhouse P M．Construct design for effcient， effective and highthroughput gene silencing in plants．Plant J， 2001， 27： 581-590．
Weigel D， Ahn JH， Blazquez MA， Borevitz JO， Christensen SK， Fankhauser C， Ferrandiz C， Kardailsky 1， Malancharuvil EJ，Neff MM．Activation tagging in Arabidopsis．Plant Physiol， 2000， 122： 1003-1013．
Whitby FG， Luecke H， Kuhn P， Somoza JR， Huete-Perez JA， Phillips JD， Hill CP， Fletterick RJ， Wang CC．Crystal structure of Tritrichomonas foetus inosine-5'-monophosphate dehydrogenase and the enzyme-product complex．Biochemistry．1997，36： 10666-10674．
Wright M， Dawson J， Dunder E， Suttie J， Reed J， Kramer C， Chang Y， Novitzky R， Wang H， Artim-moore L．Efficient biolistic transformation of maize and wheat using the phosphomannose isomerase gene， pmi， as the selectable marker．Plant Cell Rep， 2001，20：429-436．
Xiang C， Han P， Lutziger i， Wang K， Oliver DJ．A mini binary vector series for plant transformation．Plant Mol Biol， 1999， 40 711-717
Xing A Q， Zhang Z Y， Sato S， Staswick P， Clemente T．The use of the two T-DNA binary system to derive marker-free transgenic soybeans．In vitro cellular and developmental biology-plant， 2000， 36： 456-463．
Yang T J， Kwon S J， Kim J A， Park J Y， Kim J S， Jin M， Lim K B， Lim M H， Choi B S， Lim Y P Park B S．Korea Brassica Genome Project (KBGP)．Submitted to the EMBL/GenBank/DDBJ databases， 28， JUL，2006．
Ye W Z， Cao J S， Xiang X，Zeng G W．Molecular cloning and characterization of the genic male sterility related gene CYP86MF in Chinese cabbage (Brassica campestris L．ssp．chinensis Makino var communis Tsen et Lee)．Journal of Horticultural Science & Biotechnology．2003， 78(3)： 319-323．
Yoder J i， Goldsbrorlgh A P．Transformation systems for generating marker-free transgenic plants．Biotechnology， 1994．， 12：263-267．
Yu X L， Cao J S， Ye W Z， Wang Y Q．Construction of antisense gene CYP86MF plasmid vector and obtaining male sterile transformant by pollen-tuhe-mediating methodJournal of Horticultural Science & Biotechnologv 2004， 79： 833-839．
Yuan Y， Liu Y J， Wang T．A new Cre/lox system for deletion of selectable marker gene．Acta Botanica Sinica， 2004， 46： 862-866．
Zaltsman A， Yi BY， Krichevsky A， Gafni Y， Citovsky V．Yeast-plant coupled vector system for identification of nuclear proteins．Plant Physiol， 2007， 145： 1264-1271．
Zhang Q， Cao J S， Huang L， Xiang X， Yu X L．Characterization and functional analysis of a novel PCP gene BcMF5 from Brassica campestris ssp chinensis．Journal of Plant Physiology， 2008， 165： 445-55．
Zhang Q， Cao J S， Huang L， Xiang X， Yu XL．BcMF5， a pollen coat protein gene (PCP)， from Brassica rapa ssp chinensis，involved in the transcription of different lengths of 3'-UTRs of PCPs．Molecular Biology Reports， Published online： 5．， August， 2007．
Zhang R， Evans G， Rotella FJ， Westbrook EM， Beno D， Huberman E， Joachimiak A， Collart FR．Characteristics and crystal structure of bacterial inosinc-5'-monophosphate dehydrogenase．Biochemistry．1999， 38： 4691-4700．
Zuo J， Niu Q W， Moller S G， Chua N H．Chemical-regulated， site-specific DNA excision in transgenic plants．Nature Biotechnology， 2001， 19： 157-161．
白新祥，戴思兰．反义RNA技术在花色育种中的应用．植物学通报，2005， 22 (3 )： 284 -291．
本杰明·卢因．基因Ⅷ．北京；科学出版社，2005．
曹家树，曹寿椿，易清明．白菜及其相邻类群基因组DNA的RAPD分析．园艺学报，1995， 22 (1)： 47-52．
曹家树．中国白菜起源、演化和分类研究进展．园艺学年评，北京：科学出版社，1996， 137-160．
曹家树，缪颖，卢钢，曹寿椿．中国白菜各类群的分支分析和演化关系研究．园艺学报，1997，24 (1)： 35-42．
曹家树，叶纨芝，张明，曾广文．白菜核雄性不育两用系花蕾的mRNA差别显示及其cDNA差异片段分析．浙江大学学报(农业与生命科学版)， 2001， 27 (6)： 596-600．
曹家树，余小林，叶纨芝，向珣，卢钢，王永勤，黄鹂，黄科，王玲平，张弢．白菜核雄性不育相关基因的研究．中国园艺文摘，2005， 21 (5)： 39-44．
曹寿椿，李式军．矮脚黄白菜雄性不育系两用系的选育与利用．南京农学院学报，1980(1)：59-67．
曹寿椿，李式军．火白菜“矮杂一号”及雄性不育两用系的选育．园艺学报，1981，8(3)：35-41．
曹寿椿，侯喜林，张蜀宁，朱月林，刘克均．不结球白菜抗病育种的研究Ⅶ．矮抗四号新品种的选育．南京农业大学学报，1998，21 (2)： 24-29．
陈云鹏，曹家树，缪颖，叶纨芝．芸薹类蔬菜DNA遗传多样性RAPD分析．浙江大学学报(农业与生命科学版)，2000， 26 (2)： 131-136．
杜娟，朱祯，李晚忱．植物逆境诱导启动子mwcsl20的克隆及表达特性研究．作物学报，2005，31 (10)： 1328-1332．
盖树鹏，孟祥栋．转基因植物的筛选与检测．山东农业大学学报[自然科学版]， 2000，31 (1)： 95-100．
顾雪松，陈章良、朱玉贤．光敏色素与光调控．植物学报(英文版)，1997，39 (7)： 675-681．
郭晶心，周乃元，马容才，曹鸣庆．白菜类蔬菜遗传多样性的AFLP分子标记研究．农业生物技术学报，2002， 10 (2)： 138-143．
韩建明，侯喜林，徐海明，史公军，耿建峰，邓晓辉．不结球白菜(Brassica campestris ssp．chinensis Makino)种质资源SRAP遗传分化分析．作物学报，2007， 33(11)：1862-1868．
何余堂，陈宝元，傅廷栋，李殿荣，涂金星．白菜型油菜在中国的起源与进化．遗传学报，2001，28 (4)： 331-335．
候爱菊，朱延明，张晶，李杰，张彬彬．转基因植物中筛选标记基因的利用及消除．遗传，2003，25 (4)： 466-470．
胡维，向华．用PCR法直接快速筛查重组阳性克隆．中国微生态学杂志，2000， 12 (1) ： 1-2
黄鹂，曹家树，张强．十字花科植物花粉发育相关基因的研究Ⅰ．自然科学进展，2005a， 15 (8)： 907-916．
黄鹂，曹家树．十字花科植物授粉过程中花粉表达基因的研究Ⅱ．自然科学进展，2005b， 15 (11)： 1287-1296．
黄鹂，曹家树，叶意群，张豫超．甘蓝花粉特异表达的多聚半乳糖醛酸酶基因BoMF9的克隆与特征分析．农业生物技术学报，2007a， 15 (2)： 268-273．
黄鹂，曹家树，张豫超，叶意群．一个白菜花粉发育相关的新基因BcMF7的鉴定．中国科学C， 2007b， 37 (4)：479-487．
黄鹂．白菜三种雄性不育系与保持系花蕾转录组差异分析及三个花粉发育相关基因功能鉴定浙江大学博士论文，2007c．
金松恒，翁晓燕，王妮妍，李雪芹，毛伟华，蒋德安．Rubisco活化酶基因反义表达载体的构建与水稻的遗传转化．遗传，2004， 26 (6)： 881-886．
康俊根，王晓武，张国裕，张延国，娄平，方智远．利用cDNA-AFLP检测甘蓝雄性不育相关基因的时序性表达．园艺学报，2006， 33 (3)： 544-548．
孔凡江，吕慧颖，杨庆凯，赵奎军，陈庆山，宁海龙．植物转基因中应用的筛选体系．东北农业大学学报，2002， 33(2)：191-197．
廖世奇，张春梅，张雪力，孙雨葳，王黎，王晓辉．一种PCR快速鉴定重组体DNA阳性克隆及插入方向的方法．科学技术与工程，2004， 4 (02)： 89-90， 96．
李德葆，周雪平，许建平，何祖华．基因工程操作技术．上海：上海科学技术出版社，1996．
李晶，李杰，关英芝，朱延明．逆境诱导型启动子rd29A的克隆及植物表达载体的构建．植物研究，2004， 24 (1)：111-114．
刘会超，李永春，巨关生，孙振元，柴德勇．香石竹ACC氧化酶基因克隆及其反义表达载体构建．核农学报，2005， 19(6)：461-464，473．
刘慧丽，李玲．脱落酸(ABA)诱导基因表达的调控元件植物学通报，2001， 18 (3)： 276-282．
刘乐承，向珣，曹家树．白菜雄性不育相关基因BcMF4基因功能的RNAi验证．遗传，2006， 28(11)： 1428-1434．
刘乐承，向珣，曹家树．RNAi沉默BcMF3基因对菜薹花粉发育的影响．园艺学报，2007， 34 (1 )： 125-130．
娄平，王晓武，Guusje Bonnema，方智远．利用cDNA-AFLP技术克隆鉴定甘蓝显性核不育相关基因表达序列园艺学报，2003， 30 (6)： 668-672．
卢钢，曹家树陈杭．芸薹属植物分子标记技术和基因组研究进展．园艺学报1999．26(6 )，384 -390
路静，赵华燕，何奕昆，宋艳茹高等植物启动子及其应用研究进展．自然科学进展，2004， 14 (8)： 856-862．
那杰，王关林，夏然，杨怀义．草莓annfaf基因反义融合表达载体构建及转基因植株的获得．中国农业科学，2006， 39(3)：582-586．
萨姆布鲁克J，拉赛尔D．W．分子克隆实验指南，第3版．北京：科学出版社，2002．
邵宏波，梁宗锁，邵明安．高等植物对环境胁迫的适应与其胁迫信号的转导．生态学报，2005， 25 (7)： 1772-1781．
宋江华．白菜花粉发育相关基因BcMF11和BcMF12的克隆、表达及其功能验证。浙江大学博士学位论文，2006．
帅琪峰，叶纨芝，金维正，曹家树，张明，甘德芳．白菜细胞核雄性不育两用系的性状比较分析，上海农业科学，2004，20 (3)：48-51．
孙德岭，赵前程，宋文芹，陈瑞阳．白菜类蔬菜亲缘关系的AFLP分析．园艺学报，2001， 28 (4)： 331-335．
孙日飞，吴飞燕，司家钢．大白菜核雄性不育两用系小孢子发生的细胞形态学研究．园艺学报，1995，22： 153-156．
孙日飞，方智远，张淑江．萝卜胞质大白菜雄性不育系的生化分析．园艺学报， 2000， 27 (2)： 187-192．
涂红艳，刘元风．反义RNA的作用机理及其在植物基因工程领域的应用．生物磁学．2005，5 (2)： 32-34．
王关林，方宏筠．植物基因工程，第2板北京：科学出版社，2002．
王关林，孙月剑，那杰，李洪艳，方宏筠．中国转基因植物产业化的研究进展及存在问题．中国农业科学，2006， 39 (7)：1328-1335．
王玲平，曹家树，叶纨芝，向珣，周生茂十字花科植物CYP86MF基因同源序列的克隆与进化分析．遗传，2005， 27(3)：385-402．
王铃平．十字花科植物CYP86MF同源基因的结构、功能及进化关系的研究．浙江大学博士学位论文，2004．
王钦丽，卢龙斗，吴小琴，陈祖铿，林金星．花粉的保存及其生活力测定．植物学通报，2002， 29 (3)： 365-373
王亚馥，胡昌勤，林志刚等．大白菜雄性不育两用系可育株不育株的比较分析．园艺学报，1984，11 (3)： 182-186．
王永勤，曹家树，虞慧芳，叶纨芝，余小林，向珣，卢钢．白菜核雄性不育两用系生理生化特性的分析，园艺学报，2003 a，30 (2)： 212-214．
王永勤，曹家树，付庆功，余小林，叶纨芝，向珣．利用cDNA-AFLP技术分析白菜核雄性不育两用系的表达差异．中国农业科学，2003b， 36 (5)： 557-560
王永勤．白菜核不育两用系育性基因表达特征分析及其小孢子发育相关基因的分离．浙江大学博士学位论文，2003c．
王永勤，余小林，曹家树．白菜小孢子发育相关基因BcMF3的分离及序列分析．遗传学报，2004， 31 (11 )：1302-1308．
吴建勇，沈俊儒，刘平武，杨光圣．应用cDNA-AFLP研究甘蓝型油菜显性细胞核雄性不育差异表达基因．中国农业科学，2006， 39 (9)： 1921-1926．
向旭，傅家瑞．脱落酸应答基因的表达调控及其与逆境胁迫的关系．植物学通报，1998， 15 (3)： 11-16．
向珣，曹家树，叶纨芝，崔辉梅，俞建浓．白菜OguCMS相关MYB家族新基因BcMYB的克隆与特征分析．遗传，2007，29 (5)：621-628
肖玲，卢长明．以油菜脂肪酸延长酶基因fael为靶标RNAi载体的构建．中国油料作物学报，2004， 26(4) ： 6-11．
萧灿，金振华．反义基因操作及反义分子的设计原则生物工程进展．1993， 14 (2)： 27-29
许德晖，黄辰，刘利英，宋土生．高效siRNA设计的研究进展．遗传，2006， 28 (11)： 1457-1461．
燕飞，成卓敏．RNA干扰机制研究进展．遗传，2005， 27(1)： 167-172．
杨小二，孔华，郭安平，贺立卡．RNAi技术及其在植物分子生物学中的应用．分子植物育种，2005，3 (4)： 571-74．
叶纨芝，曹家树．植物雄性不育的分子机理．植物生理学通讯，2000，36 (2)： 176-181
叶纨芝．白菜细胞核雄性不育相关基因mf-CYP450的分子鉴定、克隆和序列分析．浙江大学博士学位论文，2001．
叶纨芝，曹家树，余小林，崔辉梅，黄鹂，向珣，陶甦．白菜细胞核雄性不育两用系的细胞学观察．细胞生物学杂志，2004，26 (5)：516-522．
叶意群．白菜多聚半乳糖醛酸酶基因BcMF9的功能分析．浙江大学硕士学位论文，2006．
余小林，曹家树，徐淑英．改良菜心离体培养植株再生体系的研究．实验生物学报，2001， 34 (2)： 157-161．
余小林白菜雄性不育相关基因CYP86MF的功能验证及其人公布于细的创建．浙江大学博士学论文，2002．
余小林，曹家树，许立奎，齐文雯，王小静．优化白菜类蔬菜遗传转化体系的研究．浙江大学学报(农业与生命科学版)，2005， 31 (5)： 529-534．
袁定阳，段美娟，谭炎宁，易自力，袁隆平，辛世文．共转化法获得无筛选标记的转PEPC， PPDK基因水稻恢复系纯合体．杂交水稻(HYBRID RICE) 2007，22 (2)： 57-63．
张春晓，王文棋，蒋湘宁，陈雪梅．植物基因启动子研究进展．遗传学报，2004， 31 (12)： 1455-1464．
张国裕，康俊根，张延国，程智慧，王晓武．青花菜雄性不育相关基因BoDHAR的克隆与表达分析．生物工程学报，2006a，22 (5) ： 751-756．
张国裕，康俊根，张延国，娄平，程智慧，王晓武．青花菜快速碱化因子RALF的克隆与序列分析，园艺学报，2006b， 33(3)：561-565．
张明．白菜核雄性不育基因的分子标记及遗传分析．浙江大学硕士学位论文，2000．
张强，黄鹂，曹家树．白菜多聚半乳糖醛酸酶基因BcMF6的克隆、序列分析及其表达．园艺学报，2007， 34 (1)： 117-124．
张玉，许向阳，李景富．反义RNA技术调控番茄果实耐贮性研究进展分子植物育种，2003， 1 (5/6)： 707-711．
张豫超．白菜雄性不育相关基因BcMF10的功能验证．浙江大学硕士学位论文，2006．
张豫超，叶意群，黄鹂，曹家树花粉发育相关基因BjMF6的分子克隆及其生物信息学分析．细胞生物学杂志，2007， 29(1)：158-162．
张弢．十字花科植物BcMF2和BcMF4同源基因的克隆，表达及其进化关系研究．浙江大学博士论文，2005．
赵庆臻，赵双宜，夏光敏．植物RNA沉默机制的研究进展．遗传学报，2005 ， 32 (1)：104-110．
曾雅明，何艺宾，张红心，陈亮．OsICK1基因植物反义表达载体的构建及遗传转化．厦门大学学报(自然科学版)，2006， 45 (B05)：66-69
宗建超，倪爱国，刘福森．反义技术研究进展．生物化学与生物物理进展，1992， 19 (1)： 10-14．
周长久，王鸣，吴定华，李景富．现代蔬菜育种学北京：科学技术文献出版社，1996．
朱龙付，张献龙．RNA i及其在植物遗传改良中的应用华中农业大学学报，2004 ， 23 (3) ： 472-477．
Altschul S F， Madden T L， Schaffer A A， Zhang J H， Zhang Z， Miller W and Lipman D J．Gapped BLAST and PSI-RLAST： A new generation ofprotein database search programs Nucleic Acids Res199725： 3389-3402．
Ahmed K， Davis A T， Wang H， Faust R A， Yu S， Tawfic S．Significance of protein kinase CK2 nuclear signaling in neoplasia．J Cell Biochem Suppl， 2000， 35： 130-135．
Asano T，Katagiri H， Takata K， Lin J L， Ishihara H， Inukai K， Tsukuda K， Kikuchi M．The role of N-glycosylation of GLUT1 for glucose transport activity．J Biol Chem， 1991，266： 24632-24636．
Bachem C W B， van der Hoeven R S， de Bruijn S M， Vreugdenhil D， Zabeau M， Visser R G F．Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP： analysis of gene expression during potato tuber development．Plant J， 1996， 9： 745-753．
Becker D， Kemper E， Schell J， Masterson R．New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol Biol, 1992, 20: 1195 -1197.
Bcvan M W. Binary Agrobactcrium vectors for plant transformation. Nucleic Acids Res, 1984, 12: 1811-1821.
Block A, Dangl J L, Hahlbrock K, Schulze-Lefcrt P. Functional borders, genetic fine structure, and distance requirements of cis elements mediating light responsiveness of the parsley chalcone synthase promoter. PNAS, 1990, 87: 5387-5391.
Boisson B, Giglione C, Meinnel T. Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote. J Biol Chem, 2003, 278: 43418-43429.
Borgese N, Aggujaro D, Carrera P, Pietrini G, Bassetti M. A Role for N-myristoylation in protein targeting: NADH-cytochrome b5 reductase requires myristic acid for association with outer mitochondrial but not ER membranes. J Cell Biol, 1996, 135: 1501-1513.
Bracha-Drori K, Shichrur K, Katz A, Oliva M, Angelovici R, Yalovsky S, Ohad N. Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. Plant J, 2004, 40: 419-427.
Burch-Smith T M, Schiff M, Liu Y, Dinesh-Kumar SP. Efficient virus-induced gene silencing in Arabidopsis. Plant Physiol, 2006, 142: 21-27.
Buteau H, Pezet A, Ferrag F, Perrot-Applanat M, Kelly PA, Edery M. N-glycosylation of the prolactin receptor is not required for activation of gene transcription but is crucial for its cell surface targeting. Mol Endocrinol, 1998, 12: 544-555.
Cao J S, Yu X L, Ye W Z, Lu G, Xiang X. Functional analysis of a novel male fertility CYP86MF gene in Chinese cabbage (Brassica campestris L. ssp. chinensis Makino). Plant Cell Rep, 2006, 24(12): 715-723.
Chaman A K, Alan C, Hiroshi A. Inhibition of caffeine biosynthesis in tea (Camellia sinensis) and coffee (Coffa arabica) plants by ribavirin. FEBS Letters, 2003, 554: 473-477.
Chakrabarty R, Banerjee R, Chung S M, Farman M, Citovsky V, Hogenhout S A, Tzfira T, Goodin MM. pSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-vhus interactions. Mol Plant Microbe Interact, 2007, 20: 740-750.
Chen P Y, Wang C K, Soong S C, To KY. Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Mol Breeding, 2003, 11: 287-293.
Chuang C F, Meyerowitz E M. Specific and heritable genetic interference by double -stranded RNA in Arabidopsis thaliana. PNAS,2000,97(9):4985-4999.
Citovsky V, Lee LY, Vyas S, Glick E, Chen M H, Vainstein A, Gafni Y, Gelvin SB, Tzfira T (2006) Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planla. J Mol Biol, 362: 1120-1131.
Clement S A, Tan C C, Guo J. Roles of protein kinase C and alpha-tocopherol in regulation of signal transduction for GATA-4 phosphorylarion in HL-1 cardiac muscle cells. Free Radic Biol Med, 2002, 32: 341-349.
Coutu C, Brandle J, Brown D, Brown K, Miki B, Simmonds J, Hegedus D D. pORE: a modular binary vector series suited for both monocot and dicot plant transformation. Transgenic Res, 2007, 16: 771-781.
Curtis M D, Grossniklaus U. A Gateway cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol, 2003, 133:462-469.
Dafny-Yelin M, Tzfira T. Delivery of multiple transgenes to plant cells. Plant Physiol, 2007; 145: 1118-1128.
Datla R S, Hammerlindl J K, Panchuk B, Pelcher L E, Keller W. Modified binary plani transformation vectors with the wild-type gene encoding NPTI1. Gene, 1992, 122: 383-384.
de Buck S, Peck I, de Wilde C, Marjanac G, Nolf J, de Paepe A, Depicker A. Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system. Plant Physiol, 2007, 145: 1171-1182.
de Block, Debrouwer D. Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly intergrated at the ame locus. Theor Appl Genet, 1991, 82: 257-263.
Daley M, Knauf V C, Summerfelt K R. Co-transformtion with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep, 1998, 17: 489-496.
Ding D X H, Vera J C, Heaney M L, Golde D W. N-glycosylation of the human granulocyte-macrophage colony-stimulating factor receptor alpha subunit is essential for ligand binding and signal transduction. J Biol Chem, 1995, 270: 24580-24584.
Ebinuma H, Sugita K, Matsunaga E, Yamakado M. Selection of marker-free transgenic plants using the isopentenyl transferase gene. PNAS, 1997, 94: 2117-2121.
Ebinuma H. Sugita K, Matsunaga E, Endo S, Yamada K, Komamine A. System for the removal of a selection marker and their combination with Positive marker. Plant Cell ReP, 2001, 20: 383-392.
Escobar M A, Civerolo E L, Summerfelt K R, Dandekar A M. RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis. PNAS, 2001, 98: 13437-13442.
Fernandez B, Czech M P, Meisner H. Role of protein kinase C in signal attenuation following T cell receptor engagement. J Biol Chem, 1999, 274: 20244-20250.
Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins M R, Appel R D, Bairoch A. Protein identification and analysis tools on the ExPASy Server. (In) John M. Walker (ed): The Proteomics Protocols Handbook, 2005: 571-607.
Gilbert C, Gaudry M, Naccache P H. Rapid priming of calcium mobilization and superoxide anion production in human neutrophils by substimulatory concentrations of phorbol esters: a novel role for protein kinase C and tyrosine phosphorylation in the up-modulation of signal transduction. Cell Signal, 1992, 4: 511-523.
Gleba Y, Klimyuk V, Marillonnet S. Magnifection - a new platform for expressing recombinant vaccines in plants. Vaccine, 2005, 23- 2042-2048
Goodin M M, Chakrabarty R, Banerjee R, Yelton S, DeBolt S. New gateways to discovery. Plant Physiol, 2005, 145: 1100-1109.
Grobe K, Powell L D. Role of protein kinase C in the phosphorylation of CD33 (Siglec-3) and its effect on lectin activity. Blood, 2002, 99:3188-3196.
Guo H S, Fei J F, Xie Q, Chua N H. A chemical-regulated inducible RNAi system in plants. Plant J, 2003: 34(3):383-392.
Hajdukiewicz P, Svab Z, Maliga P. The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol, 1994, 25: 989-994.
Hartley J L, Temple G F, Brasch M A. DNA cloning using in vitro site-specific recombination. Genome Res, 2000, 10: 1788-1795.
Haldrup A, Petersen S G, Okkels F T. Positive selection: a plant selection principle based on xylose isomerase, an enzyme used in the food industry. Plant Cell Rep, 1998, 18:76-81.
Higo K, Ugawa Y, Iwamoto M, Korenaga T. Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Research, 1999, 27(1): 297-300.
Hofmann K, Stoffel W. TMbase - A database of membrane spanning proteins segments. Biol.Chem Hoppe-Seyler, 1993, 374, 166.
Hoekema A, Hirsch P R, Hooykaas P J J, Schilperoort R A. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature, 1983, 303: 179-180.
Huang L, Cao J S, Zhang Y C, Ye Y Q. Characterization of a novel gene, BcMF7 that is expressed preferentially in pollen of Brassica campestris L. ssp. chinensis Makino, Sci China C Life Sci, 2007a, 50(4): 497-504.
Huang L, Cao J S, Zhang A H, Ye Y Q. Characterization of a putative pollen-specific arabinogalactan protein gene, BcMF8, from Brassica campestris ssp. chinensis. Mol Biol Rep, 2007b, Sep 5.
Huang L, Cao J S, Ye W Z, Liu T T, Jiang L X, Ye Y Q. Transcriptional differences between the male sterile mutant bcms and wild type of Brassica campestris ssp. chinensis reveal genes related to pollen development. Plant Biol, 2008, 10 (3): 342-355.
Jannick D B, Henrik N, Gunnar V H, Soren B. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol, 2004, 340:783-795.
Jeanmougin F, Thompson J D, Gouy M, Higgins D G, Gibson T J. Multiple sequence alignment with Clustal X. Trends Biochem Sci, 1998, 23,403-405.
Jefferson R A. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep, 1987a, 5: 387-405 Jefferson R A, Kavanagh T A, Bevan M W. GUS fusions: P-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J, 1987b, 6 (13): 3901-3907.
Joersbo M, Okkels F T. A novel principle for selection of positive seletion of transgenic plant cells. Plant Cell Rep, 1996, 16: 219-221.
Joersbo M, Donaldson I, Kreiberg J, Petersen S G, Brunstedr (?) Okkels F T. Analysis of mannose selection used for transformation of sugar beet. Molecular Breeding, 1998,4: 111-117.
Jones J D G, Carland F, Lim E, Ralston E, Dooner H K. Preferential transposition of the maize element activator to linked chromosomal loctions in tobacco. Plant Cell, 1990, 2: 701-707.
Kai G Y, Zhang L, Zhang H Y, Xu T F, Tang K X, Zhang H M. Marker-free: a novel tendency of transgenic plants. Acta Botanica Sinica , 2002, 44: 883-888.
Kaneko T, Katoh T, Sato S, Nakamura Y, Asamizu E, Tabata S. Structural analysis of Arabidopsis thaliana chromosome 3. @@@. Sequence features of the 4251695 bp regions covered by 90 PI, TAC and BAC clones. DNA Res. 2000, 7: 217-221.
Karimi M, Bleys A, Vanderhaeghen R, Hilson P. Building blocks for plant gene assembly. Plant Physiol, 2007, 145: 1183-1191
Kawakami Y, Kitaura J, Hartman S E, Lowell C A, Siraganian R P, Kawakami T. Regulation of protein kinase C@@@ by two protein-tyrosine kinases, Btk and Syk. PNAS, 2000, 97: 7423-7428.
Kobe F, Yvan S, Svcn D, Pierre R, Yves V P. Large-scale structural analysis of the core promoter in mammalian and plant genomes. Nucleic Acids Research, 2005, 33: 4255-4264.
Komari T, Takakura Y, Ueki J, Kato N, Ishida Y, Hiei Y. Binary vectors and super-binary vectors. Methods Mol Biol, 2006, 343: 15-42.
Komori T, Imayama T, Kato N, Ishida Y, Ueki J, Komari T. Current status of binary vectors and superbinary vectors. Plant Physiol, 2007, 145: 1155-1160.
Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T. Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J, 1996, 10:165-174.
Kumar S, Tamura K, Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics, 2004, 5: 150-163.
Lam E, Chua N H. ASF-2: A Factor That Binds to the Cauliflower Mosaic Virus 35S Promoter and a Conserved GATA Motif in Cab Promoters. Plant Cell, 1989, 1: 1147-1156.
Lassmann T, Sonnhammer E L L. Automatic assessment of alignment quality. Nucleic Acids Res, 2005, 33: 7120-7128.
Lee L Y, Kononov M E, Bassuner B, Frame B R, Wang K, Gelvin S B. Novel plant transformation vectors containing the superpromoter. Plant Physiol, 2007, 145: 1294 -1300.
Li L B, Chen N, Ramamoorthy S, Chi L, Cui X N, Wang L C, Reith M E A. The Role of N-glycosylation in function and surface trafficking of the human dopamine transporter. J Biol Chem, 2004, 279: 21012-21020.
Liu L C, Cao J S, Yu X L, Xiang X, Pei Y J. Expression of an antisense BcMF3 affects microsporogenesis and pollen tube growth in Arabidopsis. Agricultural Sciences in China, 2006, 5(5): 339-345.
Liu L C, Wang Y Q, ZHang T, Huang L, Xiang X, Cao J S. Isolation and characterization of the microspore-related gene BcMF4 in Chinese cabbage-pak-choi and its functional identification in Arabidopsis. J Horticultural Sci Biotechnol, 2007, 82(1): 133-139.
Li Q L,Yin H,Li D,Zhn H F,Zhang Y,Zhu W Wi,Isolation and Characterization of GMO Gene Promoter from Halophyte Suaeda liaotungensis K. J Genet Genomics, 2007, 34: 355-361.
Li Y Y, Cao J S, Huang L, Yu X L, Xiang X. BcMF13, a new reproductive organ-specific gene from Brassica campestris ssp. chinensis, affects pollen development. Mol Biol Rep, Published online: 27, March, 2007.
Lin R, Hiscott J. A role for casein kinase @@@ phosphorylation in the regulation of IRF-1 transcriptional activity. Mol Cell Biochem, 1999, 191: 169-180.
Liu Y G, Whittier R F. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics, 1995, 25: 674 -681.
Lizbeth H, Lu G IMP dehydrogenase: structural schizophrenia and an unusual base. Current Opinion in Chemical Biology, 2006,10:520-525.
Louwerse J D, van Lier M C, van der Steen D M, de Vlaam C M, Hooykaas P J, Vergunst A C. Stable recombinase-mediated cassette exchange in Arabidopsis using Agrobacterium tumefaciens. Plant Physiol, 2007, 145: 1282-1293.
Lucca P, Ye X D, Potrykus I. Effective selection and regeneration of transgenic rice plants with mannose as selective agent. Molecular Breeding, 2001, 7: 43-49.
Lutcke H A, Chow K C, Mickel F S, Moss K A, Kern H F, Scheele G A. Selection of AUG initiation codons differs in plants and animals. EMBO J, 1987, 6: 43-48.
Lutz K A, Azhagiri A K, Tungsuchat-Huang T, Maliga P. A guide to choosing vectors for transformation of the plastid genome of higher plants. Plant Physiol, 2007, 145: 1201-1210.
Magali Lescot, Patrice DChais, Gert Thijs, Kathleen Marchal, Yves Moreau, Yves Van de Peer, Pierre Rouz and Stephane Rombauts. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res. 2002, 30(1): 325-327.
Marco Pagni, Vassilios Ioannidis, Lorenzo Cerutti, Monique Zahn-Zabal, C. Victor Jongeneel, Jorg Hau, Olivier Martin, Dmitri Kuznetsov and Laurent Falquet. MyHits: improvements to an interactive resource for analyzing protein sequences. Nucleic Acids Research, 2007, 35, suppl_2 W433-W437.
Marillonnet S, Thoeringer C, Kandzia R, Klimyuk V, Gleba Y. Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat Biotechnol, 2005, 23: 718-723.
Matthews P R, Wang M B, Waterhouse P M, Thornton S, Fieg S J, Gubler F, Jacobsen J V. Marker gene elimination from transgenic barley, using co-transformation with adjacent 'twin T-DNAs' on a standard agrobacterium transformation vector. Molecular Breeding, 2001, 7: 195-202.
McKnight S L, Kingsbury R. Transcriptional control signals of a eukaryotic protein-coding gene. Science, 1982, 217: 316-324.
Meyer S, Nowak K, Sharma V K, Schulze J, Mendel R R, Hansch R. Vectors for RNAi technology in poplar. Plant Biol, 2004, 6: 100-103.
Negrotto D, Jolley M, Beer S, Wenck A R, Hansen G The use of phosphomannose-isomerase as a selectable marker to recover transgenic maize plants(Zea mays L.) via Agrobacterium transformation. Plant Cell Rep, 2000, 19: 798-803.
Niu L, Hcancy M L, Vcra J C, Golde D W. High-affinity binding to the GM-CSF receptor requires intact N-glycosylatinn sites in the extracellular domain of the beta subunit. Blood, 2000, 95: 3357-3362.
Emanuelsson O, Nielsen H, Brunak S, von Heijne G Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol, 2000, 300: 1005-1016.
Ohad N, Shichrur K, Yalovsky S. The analysis of protein-protein interactions in plants by bimolecular fluorescence complementation. Plant Physiol, 2007, 145: 1090-1099.
Onouchi H, Nishihama R, Kudo M. Visualization of site-specific recombination catalyzed by a recombinase from Zygosacchacomyces rouxii in arabidopsis thaliana. Mol Gen Genet, 1995, 247: 653-660.
Park J, Hill M M, Hess D, Brazil D P, Hofsteenge J, Hemmings B A. Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 and their role in regulating kinase activity. J Biol Chem, 2001, 276: 37459-37471.
Morton P, Park K. J, Obayashi T, Fujila N, Harada H, Adams-Collier C J, Nakai K. WoLF PSORT: Protein Localizalion Predictor. Nucleic Acids Research, 2007, 1-3, doi:10.1093/nar/gkm259, 2007.
Stoutjesdijk P A, Singh S P, Liu Q, Hurlstone C J, Waterhouse P A, Green A G hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing . Plant Physio), 2002, 129(4): 1723-1731.
Prestridge, D S. SIGNAL SCAN: A computer program that scans DNA sequences for eukaryotic transcriptional elements. Compul Applic Biosci, 1991,7: 203-206.
Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, Lopez R InterProScan: protein domains identifier. Nucleic Acids Res, 2005, 33: 116-120.
Gupta R, Ting JTL, Sokolov L N, Johnson S A, Luan S. A Tumor suppressor homolog, AtPTEN1, is essential for pollen development in arabidopsis. Plant Cell, 2002,14: 2495-2507.
Reed J, Privallel M, Powell M L, Meghji M, Dawson J, Dunder E, Sutthe J, Wenck A, Launis K, Kramer C, Chang Y F, Hansen G, Wright M. Phosphomannose isomerase: an efficient selectable marker for plant transformation. In Vitro Cell Dev Boil-Plant, 2001, 37: 127-132.
Robertson M. Gene Regulation: specificity and flexibility. Nature, 1987, 327: 464-466.
Rothstein S J, DiMaio J, Strand M, Rice D. Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA. PNAS, 1987, 84: 8439-8443.
Salinas J, Oeda K, Chua N-H. Two G-Box-Related Sequences Confer Different Expression Patterns in Transgenic Tobacco.Plant Cell, 1992,4: 1485-1493.
Samalova M, Brzobohaty B, Moore I. pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco. Plant J, 2005, 41: 919-935.
Sato S, Nakamura Y, Kaneko T, Katoh T, Asamizu E, Tabata S. Structural analysis of Arabidopsis thaliana chromosome 3.1. Sequence features of the regions of 4, 504, 864 bp covered by sixty PI and TAC clones. DNA Res. 2000, 7: 131-135.
Sa to S, Kaneko T, Kotani H, Nakamura Y, Asamizu E. Structural analysis of Arabidopsis thaliana chromosome 5. @@@ Sequence features of the regions of 1,456.315 bp covered by nineteen physically assigned PI and TAC clones. DNA Res 1998,5:41-54.
Shahmuradov I A, Gammerman A J, Hancock J M, Bramley P M, Solovyev V V. PlantProm: a database of plant promoter sequences. Nucleic Acids Res, 2003, 31(1): 114-117.
Smithe C J S, Watson C f, Ray J, Bird C R, Morris P C, Schuch W, Grierson D. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature, 1988, 334: 724 -726.
Solovyev V V, Shahmuradov I A. PromH: Promoters identification using orthologous genomic sequences. Nucleic Acids Res, 2003,31:3540-3545.
Stephane R, Patrice D, Marc V M, Pierre R. PlantCARE, a plant cis-acting regulatory element database. Nucleic Acids Res. 1999, 27(1): 295-6.
Spitzer B, Ben Zvi MM, Ovadis M, Marhevka E, Barkai O, Edelbaum O, Marton I, Masci T, Alon M, Morin S. Reverse genetics of floral scent: application of tobacco rattle virus-based gene silencing in petunia. Plant Physiol, 2007, 145: 1241-1250.
Sugita K, Kasaharat T, Matsunagae E,. A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J, 2000. 22: 461-469
Tada Y, Sakamoto M, Matsuoka M, Fujimura T. Expression of a monocot LHCP promoter in transgenic rice. EMBO J, 1991, 10: 1803-1808.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. Nature, 2000, 408: 823-826.
Thompson, J D, Gibson,T J, Plewniak, F, Jeanmougin, F and Higgins DG The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 1997, 24: 4876-4882.
Todd R, Tague B W. Phosphomannose isomerase: A versatile selectable marker for arobidopsis thaliana germ-line transformation. Plant Molecular Biology Reporter, 2001, 19:307-319.
Ts uchida M, Manthei E R, Alam T, Knechtle S J, Hamamy M M. Regulation of T cell receptor and CD28-induced tyrosine phosphorylation of the focal adhesion tyrosine kinases Pyk2 and Fak by protein kinase C. J Biol Chem, 2000, 275: 1344-1350.
Twell D. Pollen developmental biology. In: O'Neill S D, Roberts J A, eds, Plant Reproduction. Annual Plant Reviews, Sheffield: Sheffield Academic Press, 2002, 6: 86-153.
Tzfira T, Tian G W, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, Krichevsky A, Taylor T, Vainstein A, Citovsky V. pSAT vectors: a modular series of plasmids for fluorescent protein tagging and expression of multiple genes in plants. Plant Mol Biol, 2005,57: 503-516.
Voinnet O, Rivas S, Mestre P, Baulcombe D C. An enhanced transient expression system in plants based on suppression of gene silencing by the pl9 protein of tomato bushy stunt virus. Plant J, 2003, 33: 949-956.
Walter M, Chaban C, Schtze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C. Visualization of protein interactions in living plant cells usine bimolecular fluorescence complementation. Plant J, 2004, 40: 428-438.
Wang E M, Wagner G J. Elucidation of the functions of genes central to diterpene metabolism in tobacco trichomes using posttranscriptional gene silencing. Planta, 2003, 216: 686-691.
Wang Y Q, Cao J S, Fu Q G, Yu X L, Ye W Z, Xiang X. Differential expression analysis of genie male sterility A/B lines in Chinese cabbage -pak-choi(Brassica campestris ssp. chinensis Makino). Agricultural Sciences in China, 2003, 2: 195-199.
Wang Y Q, Ye W Z, Cao J S, Yu X L, Xiang X, Lu G Cloning and characterization of the microspore development-related gene BcMF2 in Chinese cabbage pak-choi (Brassica campestris L. ssp. chinensis Makino). Journal of Integrative Plant Biology．2005， 47(7)： 863-872．
Wang N J， Cheng Q， Ren Y G， Zhang L J， Shen G F．Studies on tissue-specific expression direction by rice 4-coumarate CoA ligase(4CL) promoter．Science Agricultura Sinica， 1996，29： 73-77．
Wesley S V， Helliwell C A， Smith N A， Wang M G B， Rouse D T， Liu Q， Gooding P S， Singh S P， Abbott D， Stoutjesdijk P A．Robinson S P， Gleave A P， Green A G， Waterhouse P M．Construct design for effcient， effective and highthroughput gene silencing in plants．Plant J， 2001， 27： 581-590．
Weigel D， Ahn JH， Blazquez MA， Borevitz JO， Christensen SK， Fankhauser C， Ferrandiz C， Kardailsky 1， Malancharuvil EJ，Neff MM．Activation tagging in Arabidopsis．Plant Physiol， 2000， 122： 1003-1013．
Whitby FG， Luecke H， Kuhn P， Somoza JR， Huete-Perez JA， Phillips JD， Hill CP， Fletterick RJ， Wang CC．Crystal structure of Tritrichomonas foetus inosine-5'-monophosphate dehydrogenase and the enzyme-product complex．Biochemistry．1997，36： 10666-10674．
Wright M， Dawson J， Dunder E， Suttie J， Reed J， Kramer C， Chang Y， Novitzky R， Wang H， Artim-moore L．Efficient biolistic transformation of maize and wheat using the phosphomannose isomerase gene， pmi， as the selectable marker．Plant Cell Rep， 2001，20：429-436．
Xiang C， Han P， Lutziger i， Wang K， Oliver DJ．A mini binary vector series for plant transformation．Plant Mol Biol， 1999， 40 711-717
Xing A Q， Zhang Z Y， Sato S， Staswick P， Clemente T．The use of the two T-DNA binary system to derive marker-free transgenic soybeans．In vitro cellular and developmental biology-plant， 2000， 36： 456-463．
Yang T J， Kwon S J， Kim J A， Park J Y， Kim J S， Jin M， Lim K B， Lim M H， Choi B S， Lim Y P Park B S．Korea Brassica Genome Project (KBGP)．Submitted to the EMBL/GenBank/DDBJ databases， 28， JUL，2006．
Ye W Z， Cao J S， Xiang X，Zeng G W．Molecular cloning and characterization of the genic male sterility related gene CYP86MF in Chinese cabbage (Brassica campestris L．ssp．chinensis Makino var communis Tsen et Lee)．Journal of Horticultural Science & Biotechnology．2003， 78(3)： 319-323．
Yoder J i， Goldsbrorlgh A P．Transformation systems for generating marker-free transgenic plants．Biotechnology， 1994．， 12：263-267．
Yu X L， Cao J S， Ye W Z， Wang Y Q．Construction of antisense gene CYP86MF plasmid vector and obtaining male sterile transformant by pollen-tuhe-mediating methodJournal of Horticultural Science & Biotechnologv 2004， 79： 833-839．
Yuan Y， Liu Y J， Wang T．A new Cre/lox system for deletion of selectable marker gene．Acta Botanica Sinica， 2004， 46： 862-866．
Zaltsman A， Yi BY， Krichevsky A， Gafni Y， Citovsky V．Yeast-plant coupled vector system for identification of nuclear proteins．Plant Physiol， 2007， 145： 1264-1271．
Zhang Q， Cao J S， Huang L， Xiang X， Yu X L．Characterization and functional analysis of a novel PCP gene BcMF5 from Brassica campestris ssp chinensis．Journal of Plant Physiology， 2008， 165： 445-55．
Zhang Q， Cao J S， Huang L， Xiang X， Yu XL．BcMF5， a pollen coat protein gene (PCP)， from Brassica rapa ssp chinensis，involved in the transcription of different lengths of 3'-UTRs of PCPs．Molecular Biology Reports， Published online： 5．， August， 2007．
Zhang R， Evans G， Rotella FJ， Westbrook EM， Beno D， Huberman E， Joachimiak A， Collart FR．Characteristics and crystal structure of bacterial inosinc-5'-monophosphate dehydrogenase．Biochemistry．1999， 38： 4691-4700．
Zuo J， Niu Q W， Moller S G， Chua N H．Chemical-regulated， site-specific DNA excision in transgenic plants．Nature Biotechnology， 2001， 19： 157-161．